Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000289.t1 | XP_003624512.1 | 97.2 | 213 | 6 | 0 | 52 | 264 | 61 | 273 | 9.60E-116 | 426.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000289.t1 | P51132 | 85.4 | 213 | 31 | 0 | 52 | 264 | 60 | 272 | 2.2e-108 | 393.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000289.t1 | G7KXA6 | 97.2 | 213 | 6 | 0 | 52 | 264 | 61 | 273 | 7.0e-116 | 426.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47123 | MS.gene000289 | PPI |
| MS.gene55486 | MS.gene000289 | PPI |
| MS.gene55480 | MS.gene000289 | PPI |
| MS.gene47121 | MS.gene000289 | PPI |
| MS.gene074110 | MS.gene000289 | PPI |
| MS.gene074111 | MS.gene000289 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000289.t1 | MTR_7g084150 | 97.183 | 213 | 6 | 0 | 52 | 264 | 61 | 273 | 4.32e-154 | 430 |
| MS.gene000289.t1 | MTR_3g082230 | 76.562 | 64 | 15 | 0 | 145 | 208 | 68 | 131 | 2.96e-26 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000289.t1 | AT5G13430 | 81.567 | 217 | 40 | 0 | 48 | 264 | 56 | 272 | 3.51e-134 | 380 |
| MS.gene000289.t1 | AT5G13440 | 82.160 | 213 | 38 | 0 | 52 | 264 | 62 | 274 | 6.17e-134 | 379 |
Find 0 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTAGATAAAAATATAAAT+TGG | + | 43389:107083-107102 | None:intergenic | 10.0% |
| !! | GTTAGATAAAAATATAAATT+GGG | + | 43389:107082-107101 | None:intergenic | 10.0% |
| !!! | ATTTATATTTTTATCTAACT+AGG | - | 43389:107083-107102 | MS.gene000289:CDS | 10.0% |
| !! | AATCAAATTAAATCAATCAA+CGG | + | 43389:107116-107135 | None:intergenic | 15.0% |
| !! | AATTATTCTATGTGTAGTTA+AGG | - | 43389:106884-106903 | MS.gene000289:CDS | 20.0% |
| !! | CAAACATTCTAATTTACAAA+CGG | + | 43389:106662-106681 | None:intergenic | 20.0% |
| ! | AACAGAGGTAAATCATAAAA+TGG | + | 43389:106820-106839 | None:intergenic | 25.0% |
| ! | AGTTGCATATTATCTTACTT+TGG | - | 43389:106973-106992 | MS.gene000289:CDS | 25.0% |
| ! | GTTGCATATTATCTTACTTT+GGG | - | 43389:106974-106993 | MS.gene000289:CDS | 25.0% |
| ! | TAAAAAACTGCATGTTTAGT+TGG | - | 43389:106712-106731 | MS.gene000289:CDS | 25.0% |
| ! | TGGTTATTGAATTGTTTAGT+TGG | - | 43389:106732-106751 | MS.gene000289:CDS | 25.0% |
| ! | TTGCATATTATCTTACTTTG+GGG | - | 43389:106975-106994 | MS.gene000289:CDS | 25.0% |
| CAAACTTCAGAACAAGTAAA+CGG | + | 43389:107352-107371 | None:intergenic | 30.0% | |
| GAAAGCTCAAAAGAATAGAT+CGG | + | 43389:106910-106929 | None:intergenic | 30.0% | |
| GGATCTCATTATGATATTTC+TGG | - | 43389:107680-107699 | MS.gene000289:CDS | 30.0% | |
| TCATATACAATCTTCGAAGA+TGG | + | 43389:107230-107249 | None:intergenic | 30.0% | |
| TCTTAAAGATTGATGAACCT+CGG | + | 43389:106592-106611 | None:intergenic | 30.0% | |
| TGCATATTATCTTACTTTGG+GGG | - | 43389:106976-106995 | MS.gene000289:CDS | 30.0% | |
| !! | ATGATATTTCTGGCAGAATT+AGG | - | 43389:107690-107709 | MS.gene000289:CDS | 30.0% |
| !! | GATTTAATTTGATTGCATCC+AGG | - | 43389:107122-107141 | MS.gene000289:intron | 30.0% |
| !!! | TAATGCTGTTTTCTTTTGTG+TGG | + | 43389:107169-107188 | None:intergenic | 30.0% |
| AAATAGTCAAGAACCCAGAA+TGG | - | 43389:107570-107589 | MS.gene000289:intron | 35.0% | |
| AACAGATGACGATGTAAAGT+TGG | - | 43389:107499-107518 | MS.gene000289:intron | 35.0% | |
| AAGTAAGGATGTTCTTGCTA+TGG | - | 43389:107388-107407 | MS.gene000289:intron | 35.0% | |
| ACCAACTTATTCATTCTTGG+AGG | - | 43389:107742-107761 | MS.gene000289:CDS | 35.0% | |
| ATGATGAACACAACCATGAA+CGG | - | 43389:107246-107265 | MS.gene000289:intron | 35.0% | |
| ATTGTGTGTATGCTATTCGT+TGG | - | 43389:107018-107037 | MS.gene000289:CDS | 35.0% | |
| CATAATGAGATCCATGACAT+GGG | + | 43389:107673-107692 | None:intergenic | 35.0% | |
| CTTAAAGATTGATGAACCTC+GGG | + | 43389:106591-106610 | None:intergenic | 35.0% | |
| GAGGAAAACAAGTTGTTGAT+CGG | - | 43389:107761-107780 | MS.gene000289:CDS | 35.0% | |
| GATCAAATGATACAGAACAG+AGG | + | 43389:106835-106854 | None:intergenic | 35.0% | |
| GATTAGAAATCATGAAGCAC+GGG | - | 43389:106550-106569 | MS.gene000289:CDS | 35.0% | |
| GGATTAGAAATCATGAAGCA+CGG | - | 43389:106549-106568 | MS.gene000289:CDS | 35.0% | |
| GGTACCAACTTATTCATTCT+TGG | - | 43389:107739-107758 | MS.gene000289:CDS | 35.0% | |
| TCATAATGAGATCCATGACA+TGG | + | 43389:107674-107693 | None:intergenic | 35.0% | |
| TCCTCCAAGAATGAATAAGT+TGG | + | 43389:107746-107765 | None:intergenic | 35.0% | |
| TTGTGTGTATGCTATTCGTT+GGG | - | 43389:107019-107038 | MS.gene000289:CDS | 35.0% | |
| TTTGCATACTTTGTCCTAAC+AGG | - | 43389:107299-107318 | MS.gene000289:intron | 35.0% | |
| ! | ATTATCTTACTTTGGGGGAT+TGG | - | 43389:106981-107000 | MS.gene000289:CDS | 35.0% |
| !! | ATTTCTGGCAGAATTAGGAA+GGG | - | 43389:107695-107714 | MS.gene000289:CDS | 35.0% |
| !! | CGAAGATGGATTTTTAACAG+CGG | + | 43389:107216-107235 | None:intergenic | 35.0% |
| !! | TATTTCTGGCAGAATTAGGA+AGG | - | 43389:107694-107713 | MS.gene000289:CDS | 35.0% |
| AACAAGTAAACGGATCAGAG+AGG | + | 43389:107342-107361 | None:intergenic | 40.0% | |
| ACTATTTCAGAATCGTGCTG+AGG | + | 43389:107557-107576 | None:intergenic | 40.0% | |
| AGCAAGAACATCCTTACTTG+CGG | + | 43389:107387-107406 | None:intergenic | 40.0% | |
| CTATTTCAGAATCGTGCTGA+GGG | + | 43389:107556-107575 | None:intergenic | 40.0% | |
| TTTAACAGCGGAAACAGTTG+CGG | + | 43389:107204-107223 | None:intergenic | 40.0% | |
| !! | TTGGCAAACAGTGTTGATGT+TGG | - | 43389:107518-107537 | MS.gene000289:intron | 40.0% |
| AAGCAAACCTTCCACCTGTT+AGG | + | 43389:107316-107335 | None:intergenic | 45.0% | |
| ACTTTGTCCTAACAGGTGGA+AGG | - | 43389:107306-107325 | MS.gene000289:intron | 45.0% | |
| AGTGACTCAGTAGCAAAACC+TGG | + | 43389:107143-107162 | None:intergenic | 45.0% | |
| GCACTACTGTCACTGTTAAG+TGG | - | 43389:107447-107466 | MS.gene000289:intron | 45.0% | |
| GCATACTTTGTCCTAACAGG+TGG | - | 43389:107302-107321 | MS.gene000289:intron | 45.0% | |
| GTGGAAAGCCAGTATTCATC+AGG | - | 43389:107471-107490 | MS.gene000289:intron | 45.0% | |
| GTTGGTACCTCCAGATTGTA+TGG | + | 43389:107728-107747 | None:intergenic | 45.0% | |
| TAATCCTGAGATCCTGTAGC+TGG | + | 43389:106535-106554 | None:intergenic | 45.0% | |
| TATGCAAATGCCCTCTTGCT+TGG | + | 43389:107287-107306 | None:intergenic | 45.0% | |
| TTAACAGCGGAAACAGTTGC+GGG | + | 43389:107203-107222 | None:intergenic | 45.0% | |
| TTAACAGTGACAGTAGTGCC+TGG | + | 43389:107446-107465 | None:intergenic | 45.0% | |
| ! | ATCGGTGTTTGCACACATCT+GGG | - | 43389:107602-107621 | MS.gene000289:intron | 45.0% |
| AAAGTCTCCAGCAGTTGGCA+AGG | + | 43389:107636-107655 | None:intergenic | 50.0% | |
| AACCATGAACGGTTTCCTCC+TGG | - | 43389:107257-107276 | MS.gene000289:intron | 50.0% | |
| AAGTCTCCAGCAGTTGGCAA+GGG | + | 43389:107635-107654 | None:intergenic | 50.0% | |
| ACCTCCAGATTGTATGGTGC+AGG | + | 43389:107722-107741 | None:intergenic | 50.0% | |
| ACCTGCACCATACAATCTGG+AGG | - | 43389:107718-107737 | MS.gene000289:CDS | 50.0% | |
| ACTGTCACTGTTAAGTGGCG+TGG | - | 43389:107452-107471 | MS.gene000289:intron | 50.0% | |
| CCAGAATGGCTCGTTGTGAT+CGG | - | 43389:107584-107603 | MS.gene000289:intron | 50.0% | |
| CGATCACAACGAGCCATTCT+GGG | + | 43389:107586-107605 | None:intergenic | 50.0% | |
| GAAACAGTTGCGGGAATGTC+AGG | + | 43389:107194-107213 | None:intergenic | 50.0% | |
| GTTCTGCGCCTGATGAATAC+TGG | + | 43389:107482-107501 | None:intergenic | 50.0% | |
| TCCTGGTGATCCAAGCAAGA+GGG | - | 43389:107274-107293 | MS.gene000289:intron | 50.0% | |
| TTGCCAACTGCTGGAGACTT+TGG | - | 43389:107635-107654 | MS.gene000289:intron | 50.0% | |
| ! | GATCGGTGTTTGCACACATC+TGG | - | 43389:107601-107620 | MS.gene000289:intron | 50.0% |
| !! | GGATGGTTTTGCCCATGTCA+TGG | - | 43389:107659-107678 | MS.gene000289:intron | 50.0% |
| AGTAGTGCCTGGCTCAATGC+TGG | + | 43389:107435-107454 | None:intergenic | 55.0% | |
| ATGAACCTCGGGAGAACCGA+CGG | + | 43389:106580-106599 | None:intergenic | 55.0% | |
| ATGAAGCACGGGACATCCGT+CGG | - | 43389:106561-106580 | MS.gene000289:CDS | 55.0% | |
| ATGCAGTGCACTCAGTCCAC+CGG | - | 43389:106506-106525 | MS.gene000289:CDS | 55.0% | |
| CACCAGGAGGAAACCGTTCA+TGG | + | 43389:107262-107281 | None:intergenic | 55.0% | |
| CCAACTGCTGGAGACTTTGG+TGG | - | 43389:107638-107657 | MS.gene000289:intron | 55.0% | |
| CCACCAAAGTCTCCAGCAGT+TGG | + | 43389:107641-107660 | None:intergenic | 55.0% | |
| CCGATCACAACGAGCCATTC+TGG | + | 43389:107587-107606 | None:intergenic | 55.0% | |
| CTCCTGGTGATCCAAGCAAG+AGG | - | 43389:107273-107292 | MS.gene000289:intron | 55.0% | |
| CTCTTGCTTGGATCACCAGG+AGG | + | 43389:107275-107294 | None:intergenic | 55.0% | |
| CTGCTGGAGACTTTGGTGGA+TGG | - | 43389:107642-107661 | MS.gene000289:intron | 55.0% | |
| GCTCAGCATGTCCGCAAGTA+AGG | - | 43389:107373-107392 | MS.gene000289:intron | 55.0% | |
| GGGACCTGCACCATACAATC+TGG | - | 43389:107715-107734 | MS.gene000289:CDS | 55.0% | |
| TCCTGAGATCCTGTAGCTGG+TGG | + | 43389:106532-106551 | None:intergenic | 55.0% | |
| TGCATTCCCTTGCCAACTGC+TGG | - | 43389:107626-107645 | MS.gene000289:intron | 55.0% | |
| ! | GATCTGTCCAGCATTGAGCC+AGG | - | 43389:107425-107444 | MS.gene000289:intron | 55.0% |
| GAGATCCTGTAGCTGGTGGC+CGG | + | 43389:106528-106547 | None:intergenic | 60.0% | |
| GCCACCAGCTACAGGATCTC+AGG | - | 43389:106528-106547 | MS.gene000289:CDS | 60.0% | |
| GCCCTCTTGCTTGGATCACC+AGG | + | 43389:107278-107297 | None:intergenic | 60.0% | |
| ATCCTGTAGCTGGTGGCCGG+TGG | + | 43389:106525-106544 | None:intergenic | 65.0% | |
| !! | GACATCCGTCGGTTCTCCCG+AGG | - | 43389:106572-106591 | MS.gene000289:CDS | 65.0% |
| GTCCACCGGCCACCAGCTAC+AGG | - | 43389:106520-106539 | MS.gene000289:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43389 | gene | 106483 | 107787 | 106483 | ID=MS.gene000289 |
| 43389 | mRNA | 106483 | 107787 | 106483 | ID=MS.gene000289.t1;Parent=MS.gene000289 |
| 43389 | exon | 107677 | 107787 | 107677 | ID=MS.gene000289.t1.exon1;Parent=MS.gene000289.t1 |
| 43389 | CDS | 107677 | 107787 | 107677 | ID=cds.MS.gene000289.t1;Parent=MS.gene000289.t1 |
| 43389 | exon | 107219 | 107258 | 107219 | ID=MS.gene000289.t1.exon2;Parent=MS.gene000289.t1 |
| 43389 | CDS | 107219 | 107258 | 107219 | ID=cds.MS.gene000289.t1;Parent=MS.gene000289.t1 |
| 43389 | exon | 106483 | 107126 | 106483 | ID=MS.gene000289.t1.exon3;Parent=MS.gene000289.t1 |
| 43389 | CDS | 106483 | 107126 | 106483 | ID=cds.MS.gene000289.t1;Parent=MS.gene000289.t1 |
| Gene Sequence |
| Protein sequence |
