AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000616

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000616.t1 MTR_4g006940 98.810 336 4 0 1 336 1 336 0.0 689
MS.gene000616.t1 MTR_4g006940 99.286 280 2 0 1 280 1 280 0.0 574
MS.gene000616.t1 MTR_4g006940 99.286 280 2 0 1 280 1 280 0.0 574
MS.gene000616.t1 MTR_2g104960 81.155 329 59 2 7 335 12 337 0.0 536
MS.gene000616.t1 MTR_3g005170 51.242 322 148 6 9 323 2 321 2.76e-105 311
MS.gene000616.t1 MTR_3g005210 50.159 315 148 6 16 323 5 317 6.79e-104 308
MS.gene000616.t1 MTR_4g077100 48.125 320 157 6 11 323 5 322 1.23e-96 289
MS.gene000616.t1 MTR_5g072620 46.519 316 162 3 15 324 3 317 2.45e-96 288
MS.gene000616.t1 MTR_8g062440 47.531 324 160 6 10 326 1 321 1.52e-95 286
MS.gene000616.t1 MTR_4g077130 47.500 320 159 7 11 323 3 320 8.83e-95 285
MS.gene000616.t1 MTR_4g009040 46.296 324 162 4 11 326 4 323 1.35e-94 284
MS.gene000616.t1 MTR_4g009040 45.732 328 162 4 11 326 4 327 5.64e-94 283
MS.gene000616.t1 MTR_8g062440 47.385 325 160 7 10 326 1 322 1.49e-93 281
MS.gene000616.t1 MTR_8g062110 47.352 321 159 6 10 323 11 328 4.63e-93 280
MS.gene000616.t1 MTR_8g062110 47.352 321 159 6 10 323 2 319 4.66e-93 280
MS.gene000616.t1 MTR_3g005170 51.254 279 128 5 9 280 2 279 7.23e-91 273
MS.gene000616.t1 MTR_4g009690 46.914 324 160 4 11 326 4 323 1.26e-88 269
MS.gene000616.t1 MTR_3g005170 46.541 318 138 6 9 323 2 290 2.02e-88 267
MS.gene000616.t1 MTR_4g009690 47.222 324 158 5 11 326 4 322 3.79e-88 268
MS.gene000616.t1 MTR_4g077100 49.458 277 132 5 11 280 5 280 1.62e-84 257
MS.gene000616.t1 MTR_3g031650 42.089 316 176 3 15 324 3 317 1.65e-83 256
MS.gene000616.t1 MTR_8g062440 48.571 280 135 5 10 282 1 278 4.50e-82 252
MS.gene000616.t1 MTR_8g062110 47.842 278 136 5 10 280 2 277 6.41e-81 248
MS.gene000616.t1 MTR_8g062110 47.842 278 136 5 10 280 2 277 7.25e-81 248
MS.gene000616.t1 MTR_5g056600 41.456 316 177 5 18 326 5 319 4.04e-80 247
MS.gene000616.t1 MTR_1g022440 41.411 326 173 11 15 327 6 326 1.87e-76 238
MS.gene000616.t1 MTR_3g031650 44.569 267 142 2 15 275 3 269 3.64e-76 235
MS.gene000616.t1 MTR_2g028620 41.176 323 177 6 14 326 7 326 3.59e-75 234
MS.gene000616.t1 MTR_2g101330 39.506 324 185 5 17 332 5 325 1.02e-74 233
MS.gene000616.t1 MTR_1g022445 41.159 328 171 12 15 327 6 326 2.37e-73 230
MS.gene000616.t1 MTR_2g028620 42.336 274 146 5 14 277 7 278 4.96e-64 206
MS.gene000616.t1 MTR_4g092080 35.385 325 189 9 18 324 15 336 5.32e-54 180
MS.gene000616.t1 MTR_7g074880 33.333 327 201 9 13 326 4 326 5.52e-54 180
MS.gene000616.t1 MTR_8g062110 41.964 224 125 4 102 323 13 233 1.20e-52 174
MS.gene000616.t1 MTR_7g074820 33.837 331 197 12 13 326 4 329 1.61e-51 174
MS.gene000616.t1 MTR_7g074730 33.435 329 200 10 13 326 4 328 2.77e-51 173
MS.gene000616.t1 MTR_4g081440 34.328 335 190 10 18 327 21 350 1.28e-49 169
MS.gene000616.t1 MTR_4g081440 34.821 336 188 11 18 327 25 355 1.66e-49 169
MS.gene000616.t1 MTR_7g074850 32.432 333 199 12 13 326 4 329 6.53e-48 164
MS.gene000616.t1 MTR_4g023730 33.846 325 197 10 17 326 8 329 2.45e-44 155
MS.gene000616.t1 MTR_7g074710 32.622 328 202 12 13 326 4 326 4.53e-44 154
MS.gene000616.t1 MTR_8g062110 41.436 181 102 3 102 280 13 191 8.35e-41 141
MS.gene000616.t1 MTR_6g406250 34.333 300 178 8 13 299 51 344 4.14e-40 144
MS.gene000616.t1 MTR_0685s0030 41.875 160 91 2 164 323 2 159 1.84e-37 132
MS.gene000616.t1 MTR_7g074870 30.000 290 186 10 50 326 3 288 7.33e-34 126
MS.gene000616.t1 MTR_5g029990 30.449 312 192 10 2 306 3 296 1.25e-33 125
MS.gene000616.t1 MTR_2g013250 27.219 338 217 10 17 328 7 341 2.40e-28 112
MS.gene000616.t1 MTR_6g406250 33.491 212 128 5 13 213 51 260 2.17e-26 105
MS.gene000616.t1 MTR_7g117190 27.059 340 212 13 17 326 7 340 2.87e-26 107
MS.gene000616.t1 MTR_0164s0070 43.262 141 61 4 17 138 5 145 3.68e-26 103
MS.gene000616.t1 MTR_2g013230 27.874 348 202 13 17 328 8 342 3.75e-26 107
MS.gene000616.t1 MTR_0685s0030 42.735 117 66 1 164 280 2 117 1.00e-25 99.8
MS.gene000616.t1 MTR_2g013260 26.393 341 208 11 17 324 8 338 2.61e-25 104
MS.gene000616.t1 MTR_7g117190 28.620 297 180 12 17 284 7 300 5.65e-25 103
MS.gene000616.t1 MTR_2g013260 26.689 296 178 9 17 280 8 296 2.48e-23 98.2
MS.gene000616.t1 MTR_3g011130 41.667 108 60 3 108 212 35 142 2.19e-18 80.9
MS.gene000616.t1 MTR_8g062710 48.750 80 31 1 17 96 20 89 1.54e-17 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000616.t1 AT1G80820 73.968 315 82 0 15 329 6 320 2.96e-171 479
MS.gene000616.t1 AT1G15950 72.871 317 86 0 10 326 6 322 3.26e-167 469
MS.gene000616.t1 AT1G15950 72.871 317 86 0 10 326 6 322 3.26e-167 469
MS.gene000616.t1 AT1G15950 72.168 309 86 0 10 318 6 314 1.18e-159 450
MS.gene000616.t1 AT1G80820 73.308 266 71 0 15 280 6 271 3.98e-138 393
MS.gene000616.t1 AT1G15950 72.222 270 75 0 11 280 7 276 1.57e-136 389
MS.gene000616.t1 AT1G15950 71.533 274 78 0 11 284 7 280 1.83e-136 389
MS.gene000616.t1 AT1G15950 72.119 269 75 0 11 279 7 275 9.31e-136 387
MS.gene000616.t1 AT1G15950 75.893 224 54 0 103 326 1 224 7.11e-115 333
MS.gene000616.t1 AT5G19440 51.090 321 148 6 11 324 4 322 3.45e-105 311
MS.gene000616.t1 AT1G51410 48.457 324 156 7 11 326 3 323 3.23e-98 293
MS.gene000616.t1 AT5G58490 44.025 318 171 3 15 326 7 323 3.44e-87 265
MS.gene000616.t1 AT1G66800 47.385 325 150 8 10 323 1 315 1.05e-86 264
MS.gene000616.t1 AT1G09480 44.037 327 168 7 10 326 1 322 4.66e-86 262
MS.gene000616.t1 AT2G33590 47.436 312 154 5 17 322 9 316 1.21e-85 261
MS.gene000616.t1 AT1G09480 44.037 327 168 7 10 326 48 369 1.28e-85 263
MS.gene000616.t1 AT1G09510 45.031 322 166 7 10 323 1 319 3.15e-85 260
MS.gene000616.t1 AT1G09490 44.410 322 168 7 13 326 4 322 9.41e-85 259
MS.gene000616.t1 AT2G02400 41.509 318 177 4 15 326 4 318 9.18e-83 254
MS.gene000616.t1 AT1G09500 44.000 325 168 8 10 323 1 322 2.88e-81 250
MS.gene000616.t1 AT1G76470 43.614 321 171 3 15 327 5 323 4.05e-80 247
MS.gene000616.t1 AT4G35420 42.587 317 172 7 17 326 8 321 9.72e-80 246
MS.gene000616.t1 AT1G68540 40.379 317 179 5 19 326 6 321 7.01e-75 234
MS.gene000616.t1 AT1G09500 46.182 275 135 7 10 274 1 272 1.34e-74 231
MS.gene000616.t1 AT2G33600 45.513 312 160 5 17 322 9 316 1.20e-73 230
MS.gene000616.t1 AT1G25460 36.825 315 191 4 19 326 6 319 1.10e-66 213
MS.gene000616.t1 AT5G42800 39.264 326 182 10 15 328 6 327 7.08e-66 213
MS.gene000616.t1 AT1G09490 41.135 282 156 6 52 326 13 291 2.89e-64 205
MS.gene000616.t1 AT1G09500 41.667 288 154 8 47 323 4 288 2.38e-61 198
MS.gene000616.t1 AT2G45400 35.976 328 191 10 16 326 39 364 5.05e-53 179
MS.gene000616.t1 AT1G61720 34.084 311 185 9 13 306 9 316 6.78e-51 172
MS.gene000616.t1 AT2G45400 37.276 279 160 7 16 279 39 317 4.97e-49 167
MS.gene000616.t1 AT4G27250 35.647 317 171 12 18 306 14 325 1.31e-47 164
MS.gene000616.t1 AT4G27250 35.922 309 171 12 18 306 14 315 5.56e-47 162
MS.gene000616.t1 AT1G68540 37.441 211 130 2 117 326 5 214 3.26e-46 156
MS.gene000616.t1 AT2G23910 32.441 299 176 8 18 302 11 297 6.25e-44 153
MS.gene000616.t1 AT2G23910 32.971 276 160 7 18 280 11 274 7.22e-44 152
MS.gene000616.t1 AT2G23910 33.212 274 158 7 18 278 11 272 1.26e-43 151
MS.gene000616.t1 AT4G30470 30.952 294 186 6 18 302 11 296 1.21e-38 139
MS.gene000616.t1 AT2G45400 32.653 294 180 9 49 326 16 307 1.39e-35 131
MS.gene000616.t1 AT5G14700 33.213 277 161 8 12 270 50 320 1.70e-35 132
MS.gene000616.t1 AT2G23910 31.679 262 154 7 54 302 14 263 1.14e-34 128
MS.gene000616.t1 AT2G45400 37.037 216 122 6 16 217 39 254 5.29e-34 125
MS.gene000616.t1 AT5G14700 32.444 225 132 6 12 221 50 269 9.07e-27 107
MS.gene000616.t1 AT4G33360 28.279 244 152 8 17 253 15 242 1.10e-17 82.8
MS.gene000616.t1 AT4G33360 28.279 244 152 8 17 253 3 230 1.37e-17 82.8
MS.gene000616.t1 AT4G33360 28.279 244 152 8 17 253 15 242 1.78e-17 82.4

Find 77 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACATCTCTACTCGTATT+TGG 0.148678 4.3:+88473029 None:intergenic
CTGGCGGGTTCATAGCTTCT+TGG 0.234598 4.3:-88473724 MS.gene000616:CDS
CACATCCTCAAGTACCTTAA+TGG 0.244324 4.3:-88472683 MS.gene000616:CDS
GTGAACCCAGTTGTGGTTCT+TGG 0.275623 4.3:-88472743 MS.gene000616:CDS
TCTTTCAAGTGATCATTCTT+TGG 0.278143 4.3:+88473405 None:intergenic
TGGTGAGGTTGTTGAAATTT+TGG 0.282212 4.3:-88472528 MS.gene000616:CDS
AAAAGAAAATCAAGTTGATT+TGG 0.293241 4.3:-88472771 MS.gene000616:CDS
ATCGATTGGAACGGTCTATA+TGG 0.308610 4.3:-88473054 MS.gene000616:CDS
GAATGATCACTTGAAAGAAT+TGG 0.322640 4.3:-88473400 MS.gene000616:CDS
TTGGCTTGTCAAACTTCTCT+TGG 0.330178 4.3:-88473705 MS.gene000616:CDS
AATATGTATGGTTTCACTCT+TGG 0.331350 4.3:+88471299 None:intergenic
TCTATCCAATCTGTTGTTCA+TGG 0.337063 4.3:-88473324 MS.gene000616:CDS
TATGAGACAAATTCTGCTTC+TGG 0.341001 4.3:-88472584 MS.gene000616:CDS
TTCTTTGTTCCATTCACTGC+TGG 0.346517 4.3:+88473128 None:intergenic
AATGTTTATATGAAACTGTT+AGG 0.352716 4.3:-88471226 MS.gene000616:CDS
TGTTGCATTCACATAAGTTT+TGG 0.352789 4.3:+88472655 None:intergenic
GAAAGAGGTTACACTGTTAG+AGG 0.360156 4.3:-88473683 MS.gene000616:CDS
AAATTTGCATACCTGGGTTT+CGG 0.363831 4.3:+88473654 None:intergenic
GTGCAGAACTGGTATTGTTA+TGG 0.378304 4.3:-88472830 MS.gene000616:intron
CAACATCTCTACTCGTATTT+GGG 0.380185 4.3:+88473030 None:intergenic
TCTTCTTGCATGGGAGGAAT+AGG 0.388850 4.3:+88471179 None:intergenic
ACAATCTTATGTTCATGTTA+AGG 0.400887 4.3:-88472633 MS.gene000616:CDS
ATCGTGTGTGTCACCGGTGC+TGG 0.407046 4.3:-88473743 MS.gene000616:CDS
CACCGGTGACACACACGATT+TGG 0.409017 4.3:+88473747 None:intergenic
TGCAGAACTGGTATTGTTAT+GGG 0.425363 4.3:-88472829 MS.gene000616:intron
TTAATTGTTGGTTGAAGTAA+TGG 0.425863 4.3:+88472719 None:intergenic
TCATATAAACATTGCTTCAC+AGG 0.433601 4.3:+88471236 None:intergenic
AATGGTCCAAGAACCACAAC+TGG 0.433883 4.3:+88472737 None:intergenic
ATTGTACTAGCATTAATTGT+TGG 0.463159 4.3:+88472707 None:intergenic
GTCAAACTTCTCTTGGAAAG+AGG 0.467940 4.3:-88473698 MS.gene000616:CDS
AAAATAACTCACAGGGTTGT+CGG 0.470932 4.3:+88473266 None:intergenic
AGAGAGGCTAACTTTGCATA+AGG 0.471199 4.3:-88473367 MS.gene000616:CDS
AAATCAAAAGCTGAAAGACT+TGG 0.471600 4.3:-88471273 MS.gene000616:CDS
GATAACACTTCATCAGTCTC+TGG 0.493312 4.3:-88473776 MS.gene000616:CDS
GACTGATGAAGTGTTATCAT+AGG 0.499014 4.3:+88473781 None:intergenic
CATATAATAGGATGAGATGT+TGG 0.502572 4.3:-88473153 MS.gene000616:intron
AACACTTACTTGGTAGGAAG+TGG 0.504048 4.3:+88472485 None:intergenic
TTATGTTCATGTTAAGGATG+TGG 0.505241 4.3:-88472627 MS.gene000616:CDS
GTTGTGTTCACTTCATCGAT+TGG 0.505971 4.3:-88473068 MS.gene000616:CDS
AAAGCTGAAAGACTTGGGAT+TGG 0.510048 4.3:-88471267 MS.gene000616:CDS
CTCACAGGGTTGTCGGTGAC+AGG 0.510560 4.3:+88473273 None:intergenic
GACCAAATCGTGTGTGTCAC+CGG 0.524328 4.3:-88473749 MS.gene000616:CDS
AGCTATGAACCCGCCAGCAC+CGG 0.525430 4.3:+88473730 None:intergenic
TTGTAGTTGATGAATCGTAT+TGG 0.525667 4.3:-88473010 MS.gene000616:CDS
GGCAACCATGAACAACAGAT+TGG 0.526807 4.3:+88473319 None:intergenic
TTAAGCAGAGTCTTCTTGCA+TGG 0.532426 4.3:+88471169 None:intergenic
ATGTTGGAGCCAGCAGTGAA+TGG 0.533675 4.3:-88473137 MS.gene000616:CDS
TGTGTGTCACCGGTGCTGGC+GGG 0.540247 4.3:-88473739 MS.gene000616:CDS
GATGAAGTGTTATCATAGGC+AGG 0.541028 4.3:+88473785 None:intergenic
GTGTGTGTCACCGGTGCTGG+CGG 0.544302 4.3:-88473740 MS.gene000616:CDS
AATCAAAAGCTGAAAGACTT+GGG 0.547219 4.3:-88471272 MS.gene000616:CDS
TTGGTAGGAAGTGGGTACTC+AGG 0.551005 4.3:+88472494 None:intergenic
TCTGTTGTTCATGGTTGCCA+TGG 0.552494 4.3:-88473315 MS.gene000616:CDS
ATATGAAACACTTACTTGGT+AGG 0.553678 4.3:+88472479 None:intergenic
TAAGCAGAGTCTTCTTGCAT+GGG 0.557099 4.3:+88471170 None:intergenic
AGAGGAACCGTCCGAAACCC+AGG 0.563011 4.3:-88473665 MS.gene000616:intron
GTTAGGAGTCTACAAGAGAA+AGG 0.565801 4.3:-88471209 MS.gene000616:CDS
GGTTGTTGTGAACCCAGTTG+TGG 0.566597 4.3:-88472750 MS.gene000616:CDS
TGGGTACTCAGGAAAATACT+TGG 0.568031 4.3:+88472505 None:intergenic
GCAGAGTCTTCTTGCATGGG+AGG 0.572180 4.3:+88471173 None:intergenic
GATCACTTGAAAGAATTGGA+AGG 0.576295 4.3:-88473396 MS.gene000616:CDS
CAGCACCATTAAGGTACTTG+AGG 0.581125 4.3:+88472678 None:intergenic
TTGCATACCTGGGTTTCGGA+CGG 0.587038 4.3:+88473658 None:intergenic
GTTCACTTCATCGATTGGAA+CGG 0.592538 4.3:-88473063 MS.gene000616:CDS
AACTGCTTTGCATCGTGGTG+AGG 0.603860 4.3:-88472543 MS.gene000616:CDS
CAGTGGCTGAACAATCAGCA+TGG 0.627131 4.3:-88472802 MS.gene000616:CDS
AGTGGCTGAACAATCAGCAT+GGG 0.640259 4.3:-88472801 MS.gene000616:CDS
AACACTTCATCAGTCTCTGG+TGG 0.644313 4.3:-88473773 MS.gene000616:CDS
CTTGCATGGGAGGAATAGGA+AGG 0.645331 4.3:+88471183 None:intergenic
AATTGGAAGGAGCAAGAGAG+AGG 0.645670 4.3:-88473383 MS.gene000616:CDS
ACACTTACTTGGTAGGAAGT+GGG 0.647055 4.3:+88472486 None:intergenic
AAGCTGTGTGGAACACACCA+TGG 0.653770 4.3:+88473298 None:intergenic
ATGGTCCAAGAACCACAACT+GGG 0.677277 4.3:+88472738 None:intergenic
TGTGAAACTGCTTTGCATCG+TGG 0.688310 4.3:-88472548 MS.gene000616:CDS
AGAGCATTGCAAGAACACCA+AGG 0.704040 4.3:-88472979 MS.gene000616:intron
CGGTGACAGGAGAAGCTGTG+TGG 0.708743 4.3:+88473286 None:intergenic
GTATTGTTATGGGAAGACAG+TGG 0.831616 4.3:-88472819 MS.gene000616:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTATGATATATTCT+TGG + chr4.3:88473272-88473291 None:intergenic 10.0%
!! ATTAAGAAAAATTTATTGAT+TGG - chr4.3:88473178-88473197 MS.gene000616:intron 10.0%
!! CATTTAGTTAATTAATATAA+GGG - chr4.3:88473115-88473134 MS.gene000616:CDS 10.0%
!! GTTAAGTTAAATAAATAATT+TGG - chr4.3:88471432-88471451 MS.gene000616:intron 10.0%
!!! TTAAGAAAAATTTATTGATT+GGG - chr4.3:88473179-88473198 MS.gene000616:intron 10.0%
!!! TTTTATTTGTATATAATTAG+AGG - chr4.3:88471527-88471546 MS.gene000616:intron 10.0%
!!! TTTTTAAGTTAATTATGTTA+TGG - chr4.3:88472514-88472533 MS.gene000616:CDS 10.0%
!! CATAATTTAACAAATACTTT+GGG - chr4.3:88473549-88473568 MS.gene000616:intron 15.0%
!! GCATTTAGTTAATTAATATA+AGG - chr4.3:88473114-88473133 MS.gene000616:CDS 15.0%
!! TATTAATTAACTAAATGCTT+AGG + chr4.3:88473112-88473131 None:intergenic 15.0%
!! TGACTTAATTATAAATTCTA+AGG + chr4.3:88472946-88472965 None:intergenic 15.0%
!! TGTGAAATTAAATTTACAAA+TGG + chr4.3:88471726-88471745 None:intergenic 15.0%
!! TTTATAAAGAGTAACTTATA+AGG - chr4.3:88471760-88471779 MS.gene000616:intron 15.0%
!!! AGTATATTAGAACTTTTAAT+TGG + chr4.3:88473154-88473173 None:intergenic 15.0%
!!! ATATATCATAAATAATTTGC+TGG - chr4.3:88473275-88473294 MS.gene000616:intron 15.0%
!!! CTTATTTAATCTTTTATTAC+TGG + chr4.3:88473348-88473367 None:intergenic 15.0%
!! AAATAATTGCTATGTTTATG+TGG - chr4.3:88471370-88471389 MS.gene000616:intron 20.0%
!! AATGTTTATATGAAACTGTT+AGG - chr4.3:88473731-88473750 MS.gene000616:CDS 20.0%
!! AGATGAACATATCATATATT+GGG - chr4.3:88473235-88473254 MS.gene000616:intron 20.0%
!! CTTATGAATTATCTAGATTA+GGG - chr4.3:88472554-88472573 MS.gene000616:CDS 20.0%
!! GCATAATTTAACAAATACTT+TGG - chr4.3:88473548-88473567 MS.gene000616:intron 20.0%
!! TAGATGAACATATCATATAT+TGG - chr4.3:88473234-88473253 MS.gene000616:intron 20.0%
!!! AAAAGAAAATCAAGTTGATT+TGG - chr4.3:88472186-88472205 MS.gene000616:intron 20.0%
!!! AAGGTGAATTAATCATTTTA+TGG - chr4.3:88471779-88471798 MS.gene000616:intron 20.0%
!!! AAGTCTTAAATTAAGTTGTT+TGG - chr4.3:88472725-88472744 MS.gene000616:CDS 20.0%
!!! AATTATAAATTCTAAGGAGT+AGG + chr4.3:88472940-88472959 None:intergenic 20.0%
!!! ACTTTTGATCTCAAAATTTT+GGG + chr4.3:88472709-88472728 None:intergenic 20.0%
!!! ATTGAGTTTTTTTGTTTGTT+AGG + chr4.3:88472848-88472867 None:intergenic 20.0%
!!! CATTTTATGGAACATATAAT+AGG - chr4.3:88471792-88471811 MS.gene000616:intron 20.0%
!!! TATTAGAACTTTTAATTGGA+TGG + chr4.3:88473150-88473169 None:intergenic 20.0%
!!! TTGAGTTTTTTTGTTTGTTA+GGG + chr4.3:88472847-88472866 None:intergenic 20.0%
!!! TTTTGATTTGAGAATATGTA+TGG + chr4.3:88473673-88473692 None:intergenic 20.0%
! AAACATAGAAAATAACTCAC+AGG + chr4.3:88471702-88471721 None:intergenic 25.0%
! AACATAGAAAATAACTCACA+GGG + chr4.3:88471701-88471720 None:intergenic 25.0%
! ACAATCTTATGTTCATGTTA+AGG - chr4.3:88472324-88472343 MS.gene000616:intron 25.0%
! ATGATATATTCTTGGTTAGT+AGG + chr4.3:88473264-88473283 None:intergenic 25.0%
! ATTACATTAAGAGTCAAAGT+TGG + chr4.3:88472913-88472932 None:intergenic 25.0%
! ATTCATCATAGCAATAGAAA+CGG + chr4.3:88473463-88473482 None:intergenic 25.0%
! ATTGTACTAGCATTAATTGT+TGG + chr4.3:88472253-88472272 None:intergenic 25.0%
! GCTTATGAATTATCTAGATT+AGG - chr4.3:88472553-88472572 MS.gene000616:CDS 25.0%
! TAGATGTACACATTGAATAA+GGG + chr4.3:88472751-88472770 None:intergenic 25.0%
! TCTATTGCTATGATGAATTA+GGG - chr4.3:88473465-88473484 MS.gene000616:intron 25.0%
! TTAGATGTACACATTGAATA+AGG + chr4.3:88472752-88472771 None:intergenic 25.0%
! TTCTATTGCTATGATGAATT+AGG - chr4.3:88473464-88473483 MS.gene000616:intron 25.0%
! TTTAAAGAAACATGCACTTA+AGG + chr4.3:88473303-88473322 None:intergenic 25.0%
!! ACTTAAGTCAATGATTTTAC+AGG - chr4.3:88472986-88473005 MS.gene000616:CDS 25.0%
!! CTTTTGATCTCAAAATTTTG+GGG + chr4.3:88472708-88472727 None:intergenic 25.0%
!! GACTTTTGATCTCAAAATTT+TGG + chr4.3:88472710-88472729 None:intergenic 25.0%
!! TTAATTGTTGGTTGAAGTAA+TGG + chr4.3:88472241-88472260 None:intergenic 25.0%
!!! AATTTTTTTTTGTGCAGAAC+TGG - chr4.3:88472116-88472135 MS.gene000616:intron 25.0%
!!! GCTAAAATTTTGAAAGTGAA+AGG + chr4.3:88473592-88473611 None:intergenic 25.0%
AAATCAAAAGCTGAAAGACT+TGG - chr4.3:88473684-88473703 MS.gene000616:CDS 30.0%
AATATGTATGGTTTCACTCT+TGG + chr4.3:88473661-88473680 None:intergenic 30.0%
AATCAAAAGCTGAAAGACTT+GGG - chr4.3:88473685-88473704 MS.gene000616:CDS 30.0%
ATATGAAACACTTACTTGGT+AGG + chr4.3:88472481-88472500 None:intergenic 30.0%
ATGACTTCTCCTAAAAATAG+TGG + chr4.3:88473054-88473073 None:intergenic 30.0%
CATATAATAGGATGAGATGT+TGG - chr4.3:88471804-88471823 MS.gene000616:intron 30.0%
GAAAAAAACTTAGTTGAGAG+AGG + chr4.3:88472599-88472618 None:intergenic 30.0%
GAATGATCACTTGAAAGAAT+TGG - chr4.3:88471557-88471576 MS.gene000616:intron 30.0%
GACTATATGAAACACTTACT+TGG + chr4.3:88472485-88472504 None:intergenic 30.0%
GTAGTGAATTAATCAAACCT+TGG + chr4.3:88471998-88472017 None:intergenic 30.0%
TCATATAAACATTGCTTCAC+AGG + chr4.3:88473724-88473743 None:intergenic 30.0%
TGTACATCTAACTTGATCTT+GGG - chr4.3:88472761-88472780 MS.gene000616:CDS 30.0%
TTAAGTGTCTTGCATATATG+AGG - chr4.3:88473526-88473545 MS.gene000616:intron 30.0%
TTATGTTCATGTTAAGGATG+TGG - chr4.3:88472330-88472349 MS.gene000616:intron 30.0%
! ATTTTACAGGTAAAAACGAG+TGG - chr4.3:88472999-88473018 MS.gene000616:CDS 30.0%
! TCTTTCAAGTGATCATTCTT+TGG + chr4.3:88471555-88471574 None:intergenic 30.0%
! TGTTGCATTCACATAAGTTT+TGG + chr4.3:88472305-88472324 None:intergenic 30.0%
!! TGACTTGTTCATTCTAAACA+GGG + chr4.3:88472807-88472826 None:intergenic 30.0%
!! TTGACTTGTTCATTCTAAAC+AGG + chr4.3:88472808-88472827 None:intergenic 30.0%
!! TTGTAGTTGATGAATCGTAT+TGG - chr4.3:88471947-88471966 MS.gene000616:intron 30.0%
AAAATAACTCACAGGGTTGT+CGG + chr4.3:88471694-88471713 None:intergenic 35.0%
AAACACCAATTGATGCAGAA+AGG - chr4.3:88472871-88472890 MS.gene000616:intron 35.0%
AAATTTGCATACCTGGGTTT+CGG + chr4.3:88471306-88471325 None:intergenic 35.0%
ACAACATCTCTACTCGTATT+TGG + chr4.3:88471931-88471950 None:intergenic 35.0%
CAACATCTCTACTCGTATTT+GGG + chr4.3:88471930-88471949 None:intergenic 35.0%
CGTGATAAATTTGCATACCT+GGG + chr4.3:88471312-88471331 None:intergenic 35.0%
GATCACTTGAAAGAATTGGA+AGG - chr4.3:88471561-88471580 MS.gene000616:intron 35.0%
GTACATCTAACTTGATCTTG+GGG - chr4.3:88472762-88472781 MS.gene000616:CDS 35.0%
GTGTACATCTAACTTGATCT+TGG - chr4.3:88472760-88472779 MS.gene000616:CDS 35.0%
TAATCTATAGTCGCAGAGAT+AGG + chr4.3:88473431-88473450 None:intergenic 35.0%
TATGAGACAAATTCTGCTTC+TGG - chr4.3:88472373-88472392 MS.gene000616:intron 35.0%
TCGTGATAAATTTGCATACC+TGG + chr4.3:88471313-88471332 None:intergenic 35.0%
TCTATCCAATCTGTTGTTCA+TGG - chr4.3:88471633-88471652 MS.gene000616:intron 35.0%
TGCAGAACTGGTATTGTTAT+GGG - chr4.3:88472128-88472147 MS.gene000616:intron 35.0%
! ACACACGATATTTGTTAACG+CGG - chr4.3:88473391-88473410 MS.gene000616:CDS 35.0%
! GACTGATGAAGTGTTATCAT+AGG + chr4.3:88471179-88471198 None:intergenic 35.0%
! TGGTGAGGTTGTTGAAATTT+TGG - chr4.3:88472429-88472448 MS.gene000616:intron 35.0%
!! CATTACCAGCCACTATTTTT+AGG - chr4.3:88473042-88473061 MS.gene000616:CDS 35.0%
!! GAACTCGAGATTTTGTTTAC+AGG - chr4.3:88472632-88472651 MS.gene000616:CDS 35.0%
AAAGCTGAAAGACTTGGGAT+TGG - chr4.3:88473690-88473709 MS.gene000616:CDS 40.0%
AACACTTACTTGGTAGGAAG+TGG + chr4.3:88472475-88472494 None:intergenic 40.0%
ACACTTACTTGGTAGGAAGT+GGG + chr4.3:88472474-88472493 None:intergenic 40.0%
ACCAATTGATGCAGAAAGGT+GGG - chr4.3:88472875-88472894 MS.gene000616:intron 40.0%
AGAGAGGCTAACTTTGCATA+AGG - chr4.3:88471590-88471609 MS.gene000616:intron 40.0%
ATCGATTGGAACGGTCTATA+TGG - chr4.3:88471903-88471922 MS.gene000616:intron 40.0%
ATCTAACTTGATCTTGGGGT+GGG - chr4.3:88472766-88472785 MS.gene000616:CDS 40.0%
CACATCCTCAAGTACCTTAA+TGG - chr4.3:88472274-88472293 MS.gene000616:intron 40.0%
CTTCTCCTAAAAATAGTGGC+TGG + chr4.3:88473050-88473069 None:intergenic 40.0%
GAAAGAGGTTACACTGTTAG+AGG - chr4.3:88471274-88471293 MS.gene000616:CDS 40.0%
GATAACACTTCATCAGTCTC+TGG - chr4.3:88471181-88471200 MS.gene000616:CDS 40.0%
GTATTGTTATGGGAAGACAG+TGG - chr4.3:88472138-88472157 MS.gene000616:intron 40.0%
GTCAAACTTCTCTTGGAAAG+AGG - chr4.3:88471259-88471278 MS.gene000616:CDS 40.0%
GTGCAGAACTGGTATTGTTA+TGG - chr4.3:88472127-88472146 MS.gene000616:intron 40.0%
GTGTGTGACAATACAATGGT+TGG + chr4.3:88473079-88473098 None:intergenic 40.0%
GTGTGTGTGTGACAATACAA+TGG + chr4.3:88473083-88473102 None:intergenic 40.0%
GTTAGGAGTCTACAAGAGAA+AGG - chr4.3:88473748-88473767 MS.gene000616:CDS 40.0%
GTTGTGTTCACTTCATCGAT+TGG - chr4.3:88471889-88471908 MS.gene000616:intron 40.0%
TAAGCAGAGTCTTCTTGCAT+GGG + chr4.3:88473790-88473809 None:intergenic 40.0%
TGGGTACTCAGGAAAATACT+TGG + chr4.3:88472455-88472474 None:intergenic 40.0%
TTAAGCAGAGTCTTCTTGCA+TGG + chr4.3:88473791-88473810 None:intergenic 40.0%
TTCTTTGTTCCATTCACTGC+TGG + chr4.3:88471832-88471851 None:intergenic 40.0%
! CGATATTTGTTAACGCGGTT+TGG - chr4.3:88473396-88473415 MS.gene000616:CDS 40.0%
! GATGAAGTGTTATCATAGGC+AGG + chr4.3:88471175-88471194 None:intergenic 40.0%
! GTTCACTTCATCGATTGGAA+CGG - chr4.3:88471894-88471913 MS.gene000616:intron 40.0%
!! AAGTTTTGGCAGCACCATTA+AGG + chr4.3:88472291-88472310 None:intergenic 40.0%
!! GTCAAAGTTGGTATACCTAG+AGG + chr4.3:88472901-88472920 None:intergenic 40.0%
!! TTGGCTTGTCAAACTTCTCT+TGG - chr4.3:88471252-88471271 MS.gene000616:CDS 40.0%
AACACTTCATCAGTCTCTGG+TGG - chr4.3:88471184-88471203 MS.gene000616:CDS 45.0%
AATGGTCCAAGAACCACAAC+TGG + chr4.3:88472223-88472242 None:intergenic 45.0%
AATTGGAAGGAGCAAGAGAG+AGG - chr4.3:88471574-88471593 MS.gene000616:intron 45.0%
AGTGGCTGAACAATCAGCAT+GGG - chr4.3:88472156-88472175 MS.gene000616:intron 45.0%
ATGGTCCAAGAACCACAACT+GGG + chr4.3:88472222-88472241 None:intergenic 45.0%
CACCAATTGATGCAGAAAGG+TGG - chr4.3:88472874-88472893 MS.gene000616:intron 45.0%
CATCTAACTTGATCTTGGGG+TGG - chr4.3:88472765-88472784 MS.gene000616:CDS 45.0%
CTTAGTTGAGAGAGGAGAGT+TGG + chr4.3:88472591-88472610 None:intergenic 45.0%
GGCAACCATGAACAACAGAT+TGG + chr4.3:88471641-88471660 None:intergenic 45.0%
GTCGCAGAGATAGGTACAAT+TGG + chr4.3:88473422-88473441 None:intergenic 45.0%
TCTAACTTGATCTTGGGGTG+GGG - chr4.3:88472767-88472786 MS.gene000616:CDS 45.0%
TCTGTTGTTCATGGTTGCCA+TGG - chr4.3:88471642-88471661 MS.gene000616:intron 45.0%
TCTTCTTGCATGGGAGGAAT+AGG + chr4.3:88473781-88473800 None:intergenic 45.0%
TGTGAAACTGCTTTGCATCG+TGG - chr4.3:88472409-88472428 MS.gene000616:intron 45.0%
! CAGCACCATTAAGGTACTTG+AGG + chr4.3:88472282-88472301 None:intergenic 45.0%
!! AGAGCATTGCAAGAACACCA+AGG - chr4.3:88471978-88471997 MS.gene000616:intron 45.0%
!! AATATTAAGATTAATTAATT+AGG - chr4.3:88472079-88472098 MS.gene000616:intron 5.0%
AACTGCTTTGCATCGTGGTG+AGG - chr4.3:88472414-88472433 MS.gene000616:intron 50.0%
AAGCTGTGTGGAACACACCA+TGG + chr4.3:88471662-88471681 None:intergenic 50.0%
ATGTTGGAGCCAGCAGTGAA+TGG - chr4.3:88471820-88471839 MS.gene000616:intron 50.0%
CAGTGGCTGAACAATCAGCA+TGG - chr4.3:88472155-88472174 MS.gene000616:intron 50.0%
CTTGCATGGGAGGAATAGGA+AGG + chr4.3:88473777-88473796 None:intergenic 50.0%
GACCAAATCGTGTGTGTCAC+CGG - chr4.3:88471208-88471227 MS.gene000616:CDS 50.0%
GCCCACCTTTCTGCATCAAT+TGG + chr4.3:88472879-88472898 None:intergenic 50.0%
GGTTGTTGTGAACCCAGTTG+TGG - chr4.3:88472207-88472226 MS.gene000616:intron 50.0%
GTGAACCCAGTTGTGGTTCT+TGG - chr4.3:88472214-88472233 MS.gene000616:intron 50.0%
TTGCATACCTGGGTTTCGGA+CGG + chr4.3:88471302-88471321 None:intergenic 50.0%
TTGGTAGGAAGTGGGTACTC+AGG + chr4.3:88472466-88472485 None:intergenic 50.0%
AACTTGATCTTGGGGTGGGG+TGG - chr4.3:88472770-88472789 MS.gene000616:CDS 55.0%
ACTTGATCTTGGGGTGGGGT+GGG - chr4.3:88472771-88472790 MS.gene000616:CDS 55.0%
ATGCAGAAAGGTGGGCCTCT+AGG - chr4.3:88472883-88472902 MS.gene000616:intron 55.0%
CACCGGTGACACACACGATT+TGG + chr4.3:88471213-88471232 None:intergenic 55.0%
GCAGAGTCTTCTTGCATGGG+AGG + chr4.3:88473787-88473806 None:intergenic 55.0%
!! CTGGCGGGTTCATAGCTTCT+TGG - chr4.3:88471233-88471252 MS.gene000616:CDS 55.0%
AGAGGAACCGTCCGAAACCC+AGG - chr4.3:88471292-88471311 MS.gene000616:CDS 60.0%
AGCTATGAACCCGCCAGCAC+CGG + chr4.3:88471230-88471249 None:intergenic 60.0%
ATCGTGTGTGTCACCGGTGC+TGG - chr4.3:88471214-88471233 MS.gene000616:CDS 60.0%
CGGTGACAGGAGAAGCTGTG+TGG + chr4.3:88471674-88471693 None:intergenic 60.0%
CTCACAGGGTTGTCGGTGAC+AGG + chr4.3:88471687-88471706 None:intergenic 60.0%
! GTGTGTGTCACCGGTGCTGG+CGG - chr4.3:88471217-88471236 MS.gene000616:CDS 65.0%
!! TGTGTGTCACCGGTGCTGGC+GGG - chr4.3:88471218-88471237 MS.gene000616:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 88471169 88473810 88471169 ID=MS.gene000616
chr4.3 mRNA 88471169 88473810 88471169 ID=MS.gene000616.t1;Parent=MS.gene000616
chr4.3 exon 88473666 88473810 88473666 ID=MS.gene000616.t1.exon1;Parent=MS.gene000616.t1
chr4.3 CDS 88473666 88473810 88473666 ID=cds.MS.gene000616.t1;Parent=MS.gene000616.t1
chr4.3 exon 88473278 88473432 88473278 ID=MS.gene000616.t1.exon2;Parent=MS.gene000616.t1
chr4.3 CDS 88473278 88473432 88473278 ID=cds.MS.gene000616.t1;Parent=MS.gene000616.t1
chr4.3 exon 88472980 88473165 88472980 ID=MS.gene000616.t1.exon3;Parent=MS.gene000616.t1
chr4.3 CDS 88472980 88473165 88472980 ID=cds.MS.gene000616.t1;Parent=MS.gene000616.t1
chr4.3 exon 88472494 88472846 88472494 ID=MS.gene000616.t1.exon4;Parent=MS.gene000616.t1
chr4.3 CDS 88472494 88472846 88472494 ID=cds.MS.gene000616.t1;Parent=MS.gene000616.t1
chr4.3 exon 88471169 88471340 88471169 ID=MS.gene000616.t1.exon5;Parent=MS.gene000616.t1
chr4.3 CDS 88471169 88471340 88471169 ID=cds.MS.gene000616.t1;Parent=MS.gene000616.t1
Gene Sequence

>MS.gene000616

ATGCCTGCCTATGATAACACTTCATCAGTCTCTGGTGGCGACCAAATCGTGTGTGTCACCGGTGCTGGCGGGTTCATAGCTTCTTGGCTTGTCAAACTTCTCTTGGAAAGAGGTTACACTGTTAGAGGAACCGTCCGAAACCCAGGTATGCAAATTTATCACGATAAGTAGCATTACTCTAAAATTAAGTATAAGAAAATGAAATAATTGCTATGTTTATGTGGTTACATAACATAACATGACATAATATCTATTTCTTGCATGTTAAGTTAAATAAATAATTTGGTAGCTTTATTAATTTCAATTTTAGTTGAAATTTATGTTATTTCTGACTTGAGTATTGATGTGTGAATTTTTGTTTTATTTGTATATAATTAGAGGATCCAAAGAATGATCACTTGAAAGAATTGGAAGGAGCAAGAGAGAGGCTAACTTTGCATAAGGTTGATCTTCTTGATCTTCAATCTATCCAATCTGTTGTTCATGGTTGCCATGGTGTGTTCCACACAGCTTCTCCTGTCACCGACAACCCTGTGAGTTATTTTCTATGTTTTCCATTTGTAAATTTAATTTCACATAGAAAATGTAACATTTATAAAGAGTAACTTATAAGGTGAATTAATCATTTTATGGAACATATAATAGGATGAGATGTTGGAGCCAGCAGTGAATGGAACAAAGAATGTGATTATAGCGTCTGCTGAAGCAAAAGTGCGACGTGTTGTGTTCACTTCATCGATTGGAACGGTCTATATGGACCCAAATACGAGTAGAGATGTTGTAGTTGATGAATCGTATTGGAGTGATTTAGAGCATTGCAAGAACACCAAGGTTTGATTAATTCACTACTTTAATAGTATTTACTATAATAGAAAATAATAGTGTGATATCATAACTTAATGAGAATATTAATATTAAGATTAATTAATTAGGTGATTAATAATTTTAATTTTTTTTTGTGCAGAACTGGTATTGTTATGGGAAGACAGTGGCTGAACAATCAGCATGGGACATAGCAAAAGAAAATCAAGTTGATTTGGTTGTTGTGAACCCAGTTGTGGTTCTTGGACCATTACTTCAACCAACAATTAATGCTAGTACAATTCACATCCTCAAGTACCTTAATGGTGCTGCCAAAACTTATGTGAATGCAACACAATCTTATGTTCATGTTAAGGATGTGGCATTAGCACATCTTCTTGTTTATGAGACAAATTCTGCTTCTGGTAGATACATATGTTGTGAAACTGCTTTGCATCGTGGTGAGGTTGTTGAAATTTTGGCCAAGTATTTTCCTGAGTACCCACTTCCTACCAAGTAAGTGTTTCATATAGTCAAATTGTCTTTTTTAAGTTAATTATGTTATGGTGCGAAATATATATATGCTTATGAATTATCTAGATTAGGGTATCTGTCAGACCAACTCTCCTCTCTCAACTAAGTTTTTTTCATCGACAAAATTTGAACTCGAGATTTTGTTTACAGGATCAAAGTGAAGTTTTACTCAGACTATTATTATACATAGTTAAAAAATTTCCCCAAAATTTTGAGATCAAAAGTCTTAAATTAAGTTGTTTGGCCCTTATTCAATGTGTACATCTAACTTGATCTTGGGGTGGGGTGGGAAAACTAGTTCCCTGTTTAGAATGAACAAGTCAAGTATTTTCAATGTGTTCCCTAACAAACAAAAAAACTCAATGTGAAACACCAATTGATGCAGAAAGGTGGGCCTCTAGGTATACCAACTTTGACTCTTAATGTAATATTTCCTACTCCTTAGAATTTATAATTAAGTCAATTTAAATTCAAGCTTCTCAACTTAAGTCAATGATTTTACAGGTAAAAACGAGTGGAGAGTACAAGTTGTATAAGTCATTACCAGCCACTATTTTTAGGAGAAGTCATTACCAACCATTGTATTGTCACACACACACACTTTACCTAAGCATTTAGTTAATTAATATAAGGGAGTGCTTAACCATCCAATTAAAAGTTCTAATATACTTTATATTAAGAAAAATTTATTGATTGGGTAAAGTAAAGTGACTTGCTTTGAAAACTTGTTTAGATGAACATATCATATATTGGGAAACCTACTAACCAAGAATATATCATAAATAATTTGCTGGTGCCTTAAGTGCATGTTTCTTTAAATAAAATATTTTTAGAACAACAACCAGTAATAAAAGATTAAATAAGAGAACAAAAGTAAAAGATAATGAACACACGATATTTGTTAACGCGGTTTGGCCAATTGTACCTATCTCTGCGACTATAGATTAGCTATAGTTCCGTTTCTATTGCTATGATGAATTAGGGTTTTAATGTTACGAGCAATATATATAGTACTACTACGATTAAGTGTCTTGCATATATGAGGCATAATTTAACAAATACTTTGGGTTTGAGAAAAGAATATGCCTTTCACTTTCAAAATTTTAGCTAAACATAATTTTTTTTTTCTTTGCAGATGTTCAGATGAAAAGAATCCAAGAGTGAAACCATACATATTCTCAAATCAAAAGCTGAAAGACTTGGGATTGGAATTCACACCTGTGAAGCAATGTTTATATGAAACTGTTAGGAGTCTACAAGAGAAAGGACACCTTCCTATTCCTCCCATGCAAGAAGACTCTGCTTAA
Protein sequence

>MS.gene000616.t1

MPAYDNTSSVSGGDQIVCVTGAGGFIASWLVKLLLERGYTVRGTVRNPEDPKNDHLKELEGARERLTLHKVDLLDLQSIQSVVHGCHGVFHTASPVTDNPDEMLEPAVNGTKNVIIASAEAKVRRVVFTSSIGTVYMDPNTSRDVVVDESYWSDLEHCKNTKNWYCYGKTVAEQSAWDIAKENQVDLVVVNPVVVLGPLLQPTINASTIHILKYLNGAAKTYVNATQSYVHVKDVALAHLLVYETNSASGRYICCETALHRGEVVEILAKYFPEYPLPTKCSDEKNPRVKPYIFSNQKLKDLGLEFTPVKQCLYETVRSLQEKGHLPIPPMQEDSA