AlfalfaGEDB | MS.gene002309

M. sativa cultivar XinJiangDaYe / MS.gene002309

KEGG: -
GO: GO:0003674 GO:0004857 GO:0008150 GO:0008428 GO:0019219 GO:0019222 GO:0030234 GO:0031323 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0098772
PFAM: PF03737.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002309.t1	XP_013462438.1	97	166	5	0	1	166	1	166	4.10E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002309.t1	Q9FH13	75.3	166	41	0	1	166	1	166	4.3e-70	265.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002309.t1	A0A072V558	97.0	166	5	0	1	166	1	166	3.0e-88	334.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene52579	MS.gene002309	PPI
MS.gene033474	MS.gene002309	PPI
MS.gene51161	MS.gene002309	PPI
MS.gene024038	MS.gene002309	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002309.t1	MTR_2g010815	96.988	166	5	1	166	1	166	2.20e-118	331
MS.gene002309.t1	MTR_2g010800	86.747	166	22	1	166	1	166	8.59e-105	297
MS.gene002309.t1	MTR_7g080040	65.060	166	58	1	166	1	166	1.59e-80	236

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002309.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene002309.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene002309.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene002309.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene002309.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene002309.t1	AT3G02770	65.060	166	58	1	166	1	166	9.27e-78	229
MS.gene002309.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228
MS.gene002309.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228

Find 37 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCTTGTTGGAGGGAATT+TGG	0.199853	2.3:+72654662	MS.gene002309:CDS
TGGCCAGTCTCGGGCATTCT+CGG	0.281731	2.3:+72654527	MS.gene002309:CDS
GCACAAAACATGGGGTGGTC+TGG	0.304278	2.3:+72654693	MS.gene002309:CDS
GGCCAGTCTCGGGCATTCTC+GGG	0.351904	2.3:+72654528	MS.gene002309:CDS
TGGTCTGGGATTGTTGTGAA+TGG	0.381293	2.3:+72654708	MS.gene002309:CDS
AAAAGGAGAAGGAAGAGTTT+TGG	0.387441	2.3:+72654611	MS.gene002309:CDS
CACAAAACATGGGGTGGTCT+GGG	0.391877	2.3:+72654694	MS.gene002309:CDS
CCCGAGACTGGCCATACATC+TGG	0.415145	2.3:-72654518	None:intergenic
GCCCCGAGAATGCCCGAGAC+TGG	0.420007	2.3:-72654530	None:intergenic
TCAAGTTGGTGTTAGAGCTT+TGG	0.422544	2.3:+72654764	MS.gene002309:CDS
AACGCAACACATTTAACAAA+TGG	0.449158	2.3:+72654468	MS.gene002309:CDS
TCCAGATGTATGGCCAGTCT+CGG	0.458278	2.3:+72654517	MS.gene002309:CDS
GGTGCAGTTGGCACAAAACA+TGG	0.464361	2.3:+72654683	MS.gene002309:CDS
CATCCAGTGTTCCAGATGTA+TGG	0.472302	2.3:+72654507	MS.gene002309:CDS
CAGTCTAGTACTATTTCAAA+TGG	0.479945	2.3:-72654925	None:intergenic
TCAAATGGACAACTCAAACT+TGG	0.482072	2.3:-72654910	None:intergenic
TGGAGGGAATTTGGTGCAGT+TGG	0.484334	2.3:+72654671	MS.gene002309:CDS
AGAGAACTTCTTGAAACAAA+AGG	0.491488	2.3:+72654594	MS.gene002309:CDS
GAGATTAATTTGTGTCAAGT+TGG	0.491931	2.3:+72654750	MS.gene002309:CDS
CATGTTGCTGTTTATGTTGG+AGG	0.502059	2.3:+72654831	MS.gene002309:CDS
AAACATGTTGCTGTTTATGT+TGG	0.508506	2.3:+72654828	MS.gene002309:CDS
TGGCCATACATCTGGAACAC+TGG	0.512769	2.3:-72654510	None:intergenic
CCAGATGTATGGCCAGTCTC+GGG	0.532810	2.3:+72654518	MS.gene002309:CDS
GTGTCACATATCTCTGCTGT+TGG	0.545822	2.3:-72654444	None:intergenic
GCCAGTCTCGGGCATTCTCG+GGG	0.553955	2.3:+72654529	MS.gene002309:CDS
AGCATGAGGTGTGCTCTTGT+TGG	0.557997	2.3:+72654651	MS.gene002309:CDS
ATGAGGTGTGCTCTTGTTGG+AGG	0.569564	2.3:+72654654	MS.gene002309:CDS
CTTCTTGAAACAAAAGGAGA+AGG	0.585218	2.3:+72654600	MS.gene002309:CDS
TGGCTCTATGCAGATAATGA+TGG	0.590987	2.3:+72654876	MS.gene002309:CDS
ATTTAACAAATGGTGACTTG+AGG	0.616446	2.3:+72654478	MS.gene002309:CDS
AGTGTTTGAAGACAATGTGT+TGG	0.617013	2.3:+72654569	MS.gene002309:CDS
GTGCAGTTGGCACAAAACAT+GGG	0.623381	2.3:+72654684	MS.gene002309:CDS
GAATGGTTGTATTAGAGATG+TGG	0.623790	2.3:+72654725	MS.gene002309:CDS
TGAGGTGTGCTCTTGTTGGA+GGG	0.651891	2.3:+72654655	MS.gene002309:CDS
AGTTGGCACAAAACATGGGG+TGG	0.672135	2.3:+72654688	MS.gene002309:CDS
TGCAGTTGGCACAAAACATG+GGG	0.672279	2.3:+72654685	MS.gene002309:CDS
TTGATGGAGGAGGAAGCATG+AGG	0.673507	2.3:+72654637	MS.gene002309:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CCACTTAAATCCAATAAAAA+CGG	+	chr2.3:72654795-72654814	MS.gene002309:CDS	25.0%
!!	ATTGTCTTCAAACACTTTTA+CGG	-	chr2.3:72654565-72654584	None:intergenic	25.0%
	AAATCCAATAAAAACGGTAC+TGG	+	chr2.3:72654801-72654820	MS.gene002309:CDS	30.0%
	AACGCAACACATTTAACAAA+TGG	+	chr2.3:72654468-72654487	MS.gene002309:CDS	30.0%
	AGAGAACTTCTTGAAACAAA+AGG	+	chr2.3:72654594-72654613	MS.gene002309:CDS	30.0%
	ATTTAACAAATGGTGACTTG+AGG	+	chr2.3:72654478-72654497	MS.gene002309:CDS	30.0%
!!	AAACATGTTGCTGTTTATGT+TGG	+	chr2.3:72654828-72654847	MS.gene002309:CDS	30.0%
!!	CCGTTTTTATTGGATTTAAG+TGG	-	chr2.3:72654798-72654817	None:intergenic	30.0%
!!	GAGATTAATTTGTGTCAAGT+TGG	+	chr2.3:72654750-72654769	MS.gene002309:CDS	30.0%
	AGTGTTTGAAGACAATGTGT+TGG	+	chr2.3:72654569-72654588	MS.gene002309:CDS	35.0%
	CTTCTTGAAACAAAAGGAGA+AGG	+	chr2.3:72654600-72654619	MS.gene002309:CDS	35.0%
	GAATGGTTGTATTAGAGATG+TGG	+	chr2.3:72654725-72654744	MS.gene002309:CDS	35.0%
	TCAAATGGACAACTCAAACT+TGG	-	chr2.3:72654913-72654932	None:intergenic	35.0%
!	AAAAGGAGAAGGAAGAGTTT+TGG	+	chr2.3:72654611-72654630	MS.gene002309:CDS	35.0%
!	AACACTTTTACGGTAACAAC+AGG	-	chr2.3:72654555-72654574	None:intergenic	35.0%
!!	CTTTTATTCGTGATGGAGAA+TGG	+	chr2.3:72654856-72654875	MS.gene002309:CDS	35.0%
!!	TTCACCAGTACCGTTTTTAT+TGG	-	chr2.3:72654808-72654827	None:intergenic	35.0%
!!!	AGAGTTTTGGTTATTGATGG+AGG	+	chr2.3:72654624-72654643	MS.gene002309:CDS	35.0%
!!!	GGAAGAGTTTTGGTTATTGA+TGG	+	chr2.3:72654621-72654640	MS.gene002309:CDS	35.0%
!	GGAGGAACTTTTATTCGTGA+TGG	+	chr2.3:72654849-72654868	MS.gene002309:CDS	40.0%
!	TGGCTCTATGCAGATAATGA+TGG	+	chr2.3:72654876-72654895	MS.gene002309:CDS	40.0%
!!	CATGTTGCTGTTTATGTTGG+AGG	+	chr2.3:72654831-72654850	MS.gene002309:CDS	40.0%
!!	TCAAGTTGGTGTTAGAGCTT+TGG	+	chr2.3:72654764-72654783	MS.gene002309:CDS	40.0%
!!!	GTTTTGGTTATTGATGGAGG+AGG	+	chr2.3:72654627-72654646	MS.gene002309:CDS	40.0%
	CATCCAGTGTTCCAGATGTA+TGG	+	chr2.3:72654507-72654526	MS.gene002309:CDS	45.0%
	GTGCAGTTGGCACAAAACAT+GGG	+	chr2.3:72654684-72654703	MS.gene002309:CDS	45.0%
	GTGTCACATATCTCTGCTGT+TGG	-	chr2.3:72654447-72654466	None:intergenic	45.0%
	TGCAGTTGGCACAAAACATG+GGG	+	chr2.3:72654685-72654704	MS.gene002309:CDS	45.0%
	TGCTCTTGTTGGAGGGAATT+TGG	+	chr2.3:72654662-72654681	MS.gene002309:CDS	45.0%
!	TGGTCTGGGATTGTTGTGAA+TGG	+	chr2.3:72654708-72654727	MS.gene002309:CDS	45.0%
	AGCATGAGGTGTGCTCTTGT+TGG	+	chr2.3:72654651-72654670	MS.gene002309:CDS	50.0%
	AGTTGGCACAAAACATGGGG+TGG	+	chr2.3:72654688-72654707	MS.gene002309:CDS	50.0%
	ATGAGGTGTGCTCTTGTTGG+AGG	+	chr2.3:72654654-72654673	MS.gene002309:CDS	50.0%
	CACAAAACATGGGGTGGTCT+GGG	+	chr2.3:72654694-72654713	MS.gene002309:CDS	50.0%
	GGTGCAGTTGGCACAAAACA+TGG	+	chr2.3:72654683-72654702	MS.gene002309:CDS	50.0%
	TCCAGATGTATGGCCAGTCT+CGG	+	chr2.3:72654517-72654536	MS.gene002309:CDS	50.0%
	TGAGGTGTGCTCTTGTTGGA+GGG	+	chr2.3:72654655-72654674	MS.gene002309:CDS	50.0%
!	TGGCCATACATCTGGAACAC+TGG	-	chr2.3:72654513-72654532	None:intergenic	50.0%
!	TTGATGGAGGAGGAAGCATG+AGG	+	chr2.3:72654637-72654656	MS.gene002309:CDS	50.0%
!!	TGGAGGGAATTTGGTGCAGT+TGG	+	chr2.3:72654671-72654690	MS.gene002309:CDS	50.0%
	CCAGATGTATGGCCAGTCTC+GGG	+	chr2.3:72654518-72654537	MS.gene002309:CDS	55.0%
	GCACAAAACATGGGGTGGTC+TGG	+	chr2.3:72654693-72654712	MS.gene002309:CDS	55.0%
	TGGCCAGTCTCGGGCATTCT+CGG	+	chr2.3:72654527-72654546	MS.gene002309:CDS	60.0%
!	CCCGAGACTGGCCATACATC+TGG	-	chr2.3:72654521-72654540	None:intergenic	60.0%
	GCCAGTCTCGGGCATTCTCG+GGG	+	chr2.3:72654529-72654548	MS.gene002309:CDS	65.0%
	GGCCAGTCTCGGGCATTCTC+GGG	+	chr2.3:72654528-72654547	MS.gene002309:CDS	65.0%
	GCCCCGAGAATGCCCGAGAC+TGG	-	chr2.3:72654533-72654552	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	72654432	72654932	72654432	ID=MS.gene002309
chr2.3	mRNA	72654432	72654932	72654432	ID=MS.gene002309.t1;Parent=MS.gene002309
chr2.3	exon	72654432	72654932	72654432	ID=MS.gene002309.t1.exon1;Parent=MS.gene002309.t1
chr2.3	CDS	72654432	72654932	72654432	ID=cds.MS.gene002309.t1;Parent=MS.gene002309.t1

Gene Sequence

>MS.gene002309

ATGGATTCTCTACCAACAGCAGAGATATGTGACACAAACGCAACACATTTAACAAATGGTGACTTGAGGATACTTCATCCAGTGTTCCAGATGTATGGCCAGTCTCGGGCATTCTCGGGGCCTGTTGTTACCGTAAAAGTGTTTGAAGACAATGTGTTGGTAAGAGAACTTCTTGAAACAAAAGGAGAAGGAAGAGTTTTGGTTATTGATGGAGGAGGAAGCATGAGGTGTGCTCTTGTTGGAGGGAATTTGGTGCAGTTGGCACAAAACATGGGGTGGTCTGGGATTGTTGTGAATGGTTGTATTAGAGATGTGGATGAGATTAATTTGTGTCAAGTTGGTGTTAGAGCTTTGGCTTCTCATCCACTTAAATCCAATAAAAACGGTACTGGTGAAAAACATGTTGCTGTTTATGTTGGAGGAACTTTTATTCGTGATGGAGAATGGCTCTATGCAGATAATGATGGTATCATTGTATCCAAGTTTGAGTTGTCCATTTGA

Protein sequence

>MS.gene002309.t1

MDSLPTAEICDTNATHLTNGDLRILHPVFQMYGQSRAFSGPVVTVKVFEDNVLVRELLETKGEGRVLVIDGGGSMRCALVGGNLVQLAQNMGWSGIVVNGCIRDVDEINLCQVGVRALASHPLKSNKNGTGEKHVAVYVGGTFIRDGEWLYADNDGIIVSKFELSI