AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene024038


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024038.t1 MTR_7g080040 100.000 166 0 0 1 166 1 166 2.09e-120 337
MS.gene024038.t1 MTR_2g010815 66.265 166 56 0 1 166 1 166 1.22e-80 236
MS.gene024038.t1 MTR_2g010800 66.265 166 56 0 1 166 1 166 2.84e-79 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene024038.t1 AT5G16450 83.735 166 27 0 1 166 1 166 5.13e-103 293
MS.gene024038.t1 AT5G16450 83.735 166 27 0 1 166 1 166 5.13e-103 293
MS.gene024038.t1 AT3G02770 83.735 166 27 0 1 166 1 166 1.41e-102 291
MS.gene024038.t1 AT5G56260 71.687 166 47 0 1 166 1 166 1.64e-85 248
MS.gene024038.t1 AT5G56260 71.687 166 47 0 1 166 1 166 1.64e-85 248
MS.gene024038.t1 AT5G56260 71.687 166 47 0 1 166 1 166 1.64e-85 248
MS.gene024038.t1 AT5G56260 71.687 166 47 0 1 166 33 198 3.70e-85 249
MS.gene024038.t1 AT5G56260 71.687 166 47 0 1 166 33 198 3.70e-85 249

Find 56 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCCATACCTTTCTTAT+TGG 0.164687 7.1:-24268849 None:intergenic
ACCTTTCTTATTGGCTTTCA+TGG 0.257848 7.1:-24268840 None:intergenic
GAGATCAATGGTTGTGATAT+TGG 0.289628 7.1:+24268794 MS.gene024038:CDS
AGATCAATGGTTGTGATATT+GGG 0.305246 7.1:+24268795 MS.gene024038:CDS
CAGTTTGCGCTGTGCAATAT+TGG 0.315099 7.1:+24268694 MS.gene024038:CDS
TTCCATTGCCTTTCTCTTCA+AGG 0.326061 7.1:-24268646 None:intergenic
TTGTTCTGTGCTTGCACTAC+AGG 0.344974 7.1:-24268725 None:intergenic
CATGTTCCCATAACTATTGC+TGG 0.375703 7.1:+24268875 MS.gene024038:CDS
TGAAGACAATGTCCTGGTTC+GGG 0.379961 7.1:+24268619 MS.gene024038:CDS
AGTTTGCGCTGTGCAATATT+GGG 0.401915 7.1:+24268695 MS.gene024038:CDS
CAATATTGCACAGCGCAAAC+TGG 0.407075 7.1:-24268693 None:intergenic
TTGAAGACAATGTCCTGGTT+CGG 0.418613 7.1:+24268618 MS.gene024038:CDS
TCCTTGAAGTAAAATGGCCT+TGG 0.424619 7.1:+24268463 None:intergenic
CCTTGTCCCAGCAATAGTTA+TGG 0.430126 7.1:-24268882 None:intergenic
CTTGTCCCAGCAATAGTTAT+GGG 0.433835 7.1:-24268881 None:intergenic
CCTTTCTTATTGGCTTTCAT+GGG 0.438890 7.1:-24268839 None:intergenic
GATATTGGGGTGAGAGCTCT+TGG 0.450174 7.1:+24268809 MS.gene024038:CDS
CGGACCTATAGTTACGTTGA+AGG 0.453624 7.1:+24268592 MS.gene024038:CDS
GAAAGCCAATAAGAAAGGTA+TGG 0.467226 7.1:+24268844 MS.gene024038:CDS
CAAGGATCGGTGATGGGGAA+TGG 0.483479 7.1:+24268900 MS.gene024038:CDS
CTGGGACAAGGATCGGTGAT+GGG 0.483554 7.1:+24268894 MS.gene024038:CDS
CGGGAGTTCCTTGAAGAGAA+AGG 0.485386 7.1:+24268638 MS.gene024038:CDS
AAGAGTGCTCGTTGTGGATG+GGG 0.493016 7.1:+24268667 MS.gene024038:CDS
GGAAGAGTGCTCGTTGTGGA+TGG 0.499526 7.1:+24268665 MS.gene024038:CDS
TCCCCGCTCAAAATCAATTG+AGG 0.512971 7.1:-24268515 None:intergenic
GCACAGAACAATGGATGGGC+AGG 0.514058 7.1:+24268737 MS.gene024038:CDS
TCAGATTTATGGTCGAAGAC+AGG 0.518932 7.1:+24268562 MS.gene024038:CDS
CCCATGAAAGCCAATAAGAA+AGG 0.521845 7.1:+24268839 MS.gene024038:CDS
CTTCAACGTAACTATAGGTC+CGG 0.524498 7.1:-24268591 None:intergenic
GCTGGGACAAGGATCGGTGA+TGG 0.524649 7.1:+24268893 MS.gene024038:CDS
AACACCTTCAACGTAACTAT+AGG 0.525623 7.1:-24268596 None:intergenic
CCCCGCTCAAAATCAATTGA+GGG 0.526263 7.1:-24268514 None:intergenic
GAAGAGTGCTCGTTGTGGAT+GGG 0.527267 7.1:+24268666 MS.gene024038:CDS
CGCATCACACACTTCGGCAG+TGG 0.539341 7.1:-24268489 None:intergenic
ACTATTGCTGGGACAAGGAT+CGG 0.546867 7.1:+24268887 MS.gene024038:CDS
AAAGCCAATAAGAAAGGTAT+GGG 0.553213 7.1:+24268845 MS.gene024038:CDS
TGCAAGCACAGAACAATGGA+TGG 0.553793 7.1:+24268732 MS.gene024038:CDS
CCATAACTATTGCTGGGACA+AGG 0.560406 7.1:+24268882 MS.gene024038:CDS
CTTCAAGGAACTCCCGAACC+AGG 0.562244 7.1:-24268631 None:intergenic
TGGGCAGGTATCATTGTGAA+TGG 0.567768 7.1:+24268752 MS.gene024038:CDS
TTCCTTGAAGAGAAAGGCAA+TGG 0.581451 7.1:+24268644 MS.gene024038:CDS
ATGTTCCCATAACTATTGCT+GGG 0.588122 7.1:+24268876 MS.gene024038:CDS
AGGGTTCGCATCACACACTT+CGG 0.594074 7.1:-24268495 None:intergenic
TGGCTTTATGCAGACACCGA+TGG 0.594724 7.1:+24268920 MS.gene024038:CDS
TGGGACAAGGATCGGTGATG+GGG 0.608225 7.1:+24268895 MS.gene024038:CDS
AGAGTGCTCGTTGTGGATGG+GGG 0.612554 7.1:+24268668 MS.gene024038:CDS
GGTGTTTGAAGACAATGTCC+TGG 0.615251 7.1:+24268613 MS.gene024038:CDS
AGAGATGTGGATGAGATCAA+TGG 0.633740 7.1:+24268782 MS.gene024038:CDS
GATCAATGGTTGTGATATTG+GGG 0.635484 7.1:+24268796 MS.gene024038:CDS
TCGAGAGATCAGAATTCCAT+CGG 0.636216 7.1:-24268936 None:intergenic
GTAGTGCAAGCACAGAACAA+TGG 0.640217 7.1:+24268728 MS.gene024038:CDS
GCAAGCACAGAACAATGGAT+GGG 0.641265 7.1:+24268733 MS.gene024038:CDS
CACTTCGGCAGTGGTAACCA+AGG 0.648262 7.1:-24268480 None:intergenic
GAATGGATGTATAAGAGATG+TGG 0.656133 7.1:+24268769 MS.gene024038:CDS
CAATGGAAGAGTGCTCGTTG+TGG 0.668797 7.1:+24268661 MS.gene024038:CDS
TTGCGCTGTGCAATATTGGG+TGG 0.699986 7.1:+24268698 MS.gene024038:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATCTGAAAAATTGGTTGA+AGG - chr7.1:24268550-24268569 None:intergenic 25.0%
! AATCTGAAAAATTGGTTGAA+GGG - chr7.1:24268549-24268568 None:intergenic 25.0%
!!! CAACCAATTTTTCAGATTTA+TGG + chr7.1:24268551-24268570 MS.gene024038:CDS 25.0%
AGATCAATGGTTGTGATATT+GGG + chr7.1:24268795-24268814 MS.gene024038:CDS 30.0%
CGACCATAAATCTGAAAAAT+TGG - chr7.1:24268557-24268576 None:intergenic 30.0%
! AAAGCCAATAAGAAAGGTAT+GGG + chr7.1:24268845-24268864 MS.gene024038:CDS 30.0%
AACACCTTCAACGTAACTAT+AGG - chr7.1:24268599-24268618 None:intergenic 35.0%
ATGTTCCCATAACTATTGCT+GGG + chr7.1:24268876-24268895 MS.gene024038:CDS 35.0%
GAAAGCCAATAAGAAAGGTA+TGG + chr7.1:24268844-24268863 MS.gene024038:CDS 35.0%
GAATGGATGTATAAGAGATG+TGG + chr7.1:24268769-24268788 MS.gene024038:CDS 35.0%
GAGATCAATGGTTGTGATAT+TGG + chr7.1:24268794-24268813 MS.gene024038:CDS 35.0%
GATCAATGGTTGTGATATTG+GGG + chr7.1:24268796-24268815 MS.gene024038:CDS 35.0%
TTCTCCCATACCTTTCTTAT+TGG - chr7.1:24268852-24268871 None:intergenic 35.0%
!! AACCCTCAATTGATTTTGAG+CGG + chr7.1:24268512-24268531 MS.gene024038:CDS 35.0%
!! ACCTTTCTTATTGGCTTTCA+TGG - chr7.1:24268843-24268862 None:intergenic 35.0%
!! CCTTTCTTATTGGCTTTCAT+GGG - chr7.1:24268842-24268861 None:intergenic 35.0%
AGAGATGTGGATGAGATCAA+TGG + chr7.1:24268782-24268801 MS.gene024038:CDS 40.0%
AGTTTGCGCTGTGCAATATT+GGG + chr7.1:24268695-24268714 MS.gene024038:CDS 40.0%
CATGTTCCCATAACTATTGC+TGG + chr7.1:24268875-24268894 MS.gene024038:CDS 40.0%
CCCATGAAAGCCAATAAGAA+AGG + chr7.1:24268839-24268858 MS.gene024038:CDS 40.0%
CTTCAACGTAACTATAGGTC+CGG - chr7.1:24268594-24268613 None:intergenic 40.0%
CTTGTCCCAGCAATAGTTAT+GGG - chr7.1:24268884-24268903 None:intergenic 40.0%
TCAGATTTATGGTCGAAGAC+AGG + chr7.1:24268562-24268581 MS.gene024038:CDS 40.0%
TCGAGAGATCAGAATTCCAT+CGG - chr7.1:24268939-24268958 None:intergenic 40.0%
TTCCATTGCCTTTCTCTTCA+AGG - chr7.1:24268649-24268668 None:intergenic 40.0%
TTCCTTGAAGAGAAAGGCAA+TGG + chr7.1:24268644-24268663 MS.gene024038:CDS 40.0%
TTGAAGACAATGTCCTGGTT+CGG + chr7.1:24268618-24268637 MS.gene024038:CDS 40.0%
!! ACCCTCAATTGATTTTGAGC+GGG + chr7.1:24268513-24268532 MS.gene024038:CDS 40.0%
ACTATTGCTGGGACAAGGAT+CGG + chr7.1:24268887-24268906 MS.gene024038:CDS 45.0%
CAATATTGCACAGCGCAAAC+TGG - chr7.1:24268696-24268715 None:intergenic 45.0%
CAGTTTGCGCTGTGCAATAT+TGG + chr7.1:24268694-24268713 MS.gene024038:CDS 45.0%
CCATAACTATTGCTGGGACA+AGG + chr7.1:24268882-24268901 MS.gene024038:CDS 45.0%
CCCCGCTCAAAATCAATTGA+GGG - chr7.1:24268517-24268536 None:intergenic 45.0%
CCTTGTCCCAGCAATAGTTA+TGG - chr7.1:24268885-24268904 None:intergenic 45.0%
CGGACCTATAGTTACGTTGA+AGG + chr7.1:24268592-24268611 MS.gene024038:CDS 45.0%
GCAAGCACAGAACAATGGAT+GGG + chr7.1:24268733-24268752 MS.gene024038:CDS 45.0%
GGTGTTTGAAGACAATGTCC+TGG + chr7.1:24268613-24268632 MS.gene024038:CDS 45.0%
GTAGTGCAAGCACAGAACAA+TGG + chr7.1:24268728-24268747 MS.gene024038:CDS 45.0%
TCCCCGCTCAAAATCAATTG+AGG - chr7.1:24268518-24268537 None:intergenic 45.0%
TGAAGACAATGTCCTGGTTC+GGG + chr7.1:24268619-24268638 MS.gene024038:CDS 45.0%
TGCAAGCACAGAACAATGGA+TGG + chr7.1:24268732-24268751 MS.gene024038:CDS 45.0%
TGGGCAGGTATCATTGTGAA+TGG + chr7.1:24268752-24268771 MS.gene024038:CDS 45.0%
TTGTTCTGTGCTTGCACTAC+AGG - chr7.1:24268728-24268747 None:intergenic 45.0%
!! CCCTCAATTGATTTTGAGCG+GGG + chr7.1:24268514-24268533 MS.gene024038:CDS 45.0%
AGGGTTCGCATCACACACTT+CGG - chr7.1:24268498-24268517 None:intergenic 50.0%
CGGGAGTTCCTTGAAGAGAA+AGG + chr7.1:24268638-24268657 MS.gene024038:CDS 50.0%
GATATTGGGGTGAGAGCTCT+TGG + chr7.1:24268809-24268828 MS.gene024038:CDS 50.0%
TTGCGCTGTGCAATATTGGG+TGG + chr7.1:24268698-24268717 MS.gene024038:CDS 50.0%
! GGTCGAAGACAGGTCTTTTC+CGG + chr7.1:24268572-24268591 MS.gene024038:CDS 50.0%
!! AAGAGTGCTCGTTGTGGATG+GGG + chr7.1:24268667-24268686 MS.gene024038:CDS 50.0%
!! CAATGGAAGAGTGCTCGTTG+TGG + chr7.1:24268661-24268680 MS.gene024038:CDS 50.0%
!! GAAGAGTGCTCGTTGTGGAT+GGG + chr7.1:24268666-24268685 MS.gene024038:CDS 50.0%
!! TGGCTTTATGCAGACACCGA+TGG + chr7.1:24268920-24268939 MS.gene024038:CDS 50.0%
CACTTCGGCAGTGGTAACCA+AGG - chr7.1:24268483-24268502 None:intergenic 55.0%
CTGGGACAAGGATCGGTGAT+GGG + chr7.1:24268894-24268913 MS.gene024038:CDS 55.0%
CTTCAAGGAACTCCCGAACC+AGG - chr7.1:24268634-24268653 None:intergenic 55.0%
GCACAGAACAATGGATGGGC+AGG + chr7.1:24268737-24268756 MS.gene024038:CDS 55.0%
TGGGACAAGGATCGGTGATG+GGG + chr7.1:24268895-24268914 MS.gene024038:CDS 55.0%
! AGAGTGCTCGTTGTGGATGG+GGG + chr7.1:24268668-24268687 MS.gene024038:CDS 55.0%
!! CAAGGATCGGTGATGGGGAA+TGG + chr7.1:24268900-24268919 MS.gene024038:CDS 55.0%
!! GGAAGAGTGCTCGTTGTGGA+TGG + chr7.1:24268665-24268684 MS.gene024038:CDS 55.0%
CGCATCACACACTTCGGCAG+TGG - chr7.1:24268492-24268511 None:intergenic 60.0%
GCTGGGACAAGGATCGGTGA+TGG + chr7.1:24268893-24268912 MS.gene024038:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 24268476 24268976 24268476 ID=MS.gene024038
chr7.1 mRNA 24268476 24268976 24268476 ID=MS.gene024038.t1;Parent=MS.gene024038
chr7.1 exon 24268476 24268976 24268476 ID=MS.gene024038.t1.exon1;Parent=MS.gene024038.t1
chr7.1 CDS 24268476 24268976 24268476 ID=cds.MS.gene024038.t1;Parent=MS.gene024038.t1
Gene Sequence

>MS.gene024038

ATGGCCTTGGTTACCACTGCCGAAGTGTGTGATGCGAACCCTCAATTGATTTTGAGCGGGGAGCTTCGTGCCCTTCAACCAATTTTTCAGATTTATGGTCGAAGACAGGTCTTTTCCGGACCTATAGTTACGTTGAAGGTGTTTGAAGACAATGTCCTGGTTCGGGAGTTCCTTGAAGAGAAAGGCAATGGAAGAGTGCTCGTTGTGGATGGGGGTGCCAGTTTGCGCTGTGCAATATTGGGTGGTAATCCTGTAGTGCAAGCACAGAACAATGGATGGGCAGGTATCATTGTGAATGGATGTATAAGAGATGTGGATGAGATCAATGGTTGTGATATTGGGGTGAGAGCTCTTGGTTCTCATCCCATGAAAGCCAATAAGAAAGGTATGGGAGAAAAACATGTTCCCATAACTATTGCTGGGACAAGGATCGGTGATGGGGAATGGCTTTATGCAGACACCGATGGAATTCTGATCTCTCGAACAGAGCTTTCTGTTTGA

Protein sequence

>MS.gene024038.t1

MALVTTAEVCDANPQLILSGELRALQPIFQIYGRRQVFSGPIVTLKVFEDNVLVREFLEEKGNGRVLVVDGGASLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALGSHPMKANKKGMGEKHVPITIAGTRIGDGEWLYADTDGILISRTELSV