AlfalfaGEDB | MS.gene033474

M. sativa cultivar XinJiangDaYe / MS.gene033474

KEGG: -
GO: GO:0003674 GO:0004857 GO:0008150 GO:0008428 GO:0019219 GO:0019222 GO:0030234 GO:0031323 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0098772
PFAM: PF03737.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033474.t1	XP_003624176.1	100	166	0	0	1	166	1	166	2.80E-89	337.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033474.t1	Q9FFE0	83.7	166	27	0	1	166	1	166	3.0e-79	295.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033474.t1	B7FGQ6	100.0	166	0	0	1	166	1	166	2.0e-89	337.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene51161	MS.gene033474	PPI
MS.gene02320	MS.gene033474	PPI
MS.gene033474	MS.gene002309	PPI
MS.gene033474	MS.gene01288	PPI
MS.gene024038	MS.gene033474	PPI
MS.gene033474	MS.gene01699	PPI
MS.gene033474	MS.gene52579	PPI
MS.gene033474	MS.gene024038	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033474.t1	MTR_7g080040	100.000	166	0	1	166	1	166	2.09e-120	337
MS.gene033474.t1	MTR_2g010815	66.265	166	56	1	166	1	166	1.22e-80	236
MS.gene033474.t1	MTR_2g010800	66.265	166	56	1	166	1	166	2.84e-79	233

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033474.t1	AT5G16450	83.735	166	27	1	166	1	166	5.13e-103	293
MS.gene033474.t1	AT5G16450	83.735	166	27	1	166	1	166	5.13e-103	293
MS.gene033474.t1	AT3G02770	83.735	166	27	1	166	1	166	1.41e-102	291
MS.gene033474.t1	AT5G56260	71.687	166	47	1	166	1	166	1.64e-85	248
MS.gene033474.t1	AT5G56260	71.687	166	47	1	166	1	166	1.64e-85	248
MS.gene033474.t1	AT5G56260	71.687	166	47	1	166	1	166	1.64e-85	248
MS.gene033474.t1	AT5G56260	71.687	166	47	1	166	33	198	3.70e-85	249
MS.gene033474.t1	AT5G56260	71.687	166	47	1	166	33	198	3.70e-85	249

Find 56 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTCTTGTTGGCTTTCA+TGG	0.170552	7.2:-25739318	None:intergenic
GAGATCAATGGCTGTGATAT+TGG	0.294430	7.2:+25739272	MS.gene033474:CDS
TTCTCCCATACCTTTCTTGT+TGG	0.301730	7.2:-25739327	None:intergenic
CAGTTTGCGCTGTGCAATAT+TGG	0.315099	7.2:+25739172	MS.gene033474:CDS
TTCCATTGCCTTTCTCTTCA+AGG	0.326061	7.2:-25739124	None:intergenic
TTGTTCTGTGCTTGCACTAC+AGG	0.344974	7.2:-25739203	None:intergenic
AGATCAATGGCTGTGATATT+GGG	0.360638	7.2:+25739273	MS.gene033474:CDS
CATGTTCCCATAACTATTGC+TGG	0.375703	7.2:+25739353	MS.gene033474:CDS
TGAAGACAATGTCCTGGTTC+GGG	0.379961	7.2:+25739097	MS.gene033474:CDS
AGTTTGCGCTGTGCAATATT+GGG	0.401915	7.2:+25739173	MS.gene033474:CDS
CAATATTGCACAGCGCAAAC+TGG	0.407075	7.2:-25739171	None:intergenic
CCTTTCTTGTTGGCTTTCAT+GGG	0.409967	7.2:-25739317	None:intergenic
TTGAAGACAATGTCCTGGTT+CGG	0.418613	7.2:+25739096	MS.gene033474:CDS
TCCTTGAAGTAAAATGGCCT+TGG	0.424619	7.2:+25738941	None:intergenic
CCTTGTCCCAGCAATAGTTA+TGG	0.430126	7.2:-25739360	None:intergenic
GATATTGGGGTGAGAGCTCT+TGG	0.432029	7.2:+25739287	MS.gene033474:CDS
CTTGTCCCAGCAATAGTTAT+GGG	0.433835	7.2:-25739359	None:intergenic
CGGACCTATAGTTACGTTGA+AGG	0.453624	7.2:+25739070	MS.gene033474:CDS
TCCCCACTCAAAATTAATTG+AGG	0.483439	7.2:-25738993	None:intergenic
CAAGGATCGGTGATGGGGAA+TGG	0.483479	7.2:+25739378	MS.gene033474:CDS
CTGGGACAAGGATCGGTGAT+GGG	0.483554	7.2:+25739372	MS.gene033474:CDS
CGGGAGTTCCTTGAAGAGAA+AGG	0.485386	7.2:+25739116	MS.gene033474:CDS
CCCCACTCAAAATTAATTGA+GGG	0.489898	7.2:-25738992	None:intergenic
AAGAGTGCTCGTTGTGGATG+GGG	0.493016	7.2:+25739145	MS.gene033474:CDS
GGAAGAGTGCTCGTTGTGGA+TGG	0.499526	7.2:+25739143	MS.gene033474:CDS
GAAAGCCAACAAGAAAGGTA+TGG	0.500298	7.2:+25739322	MS.gene033474:CDS
GCACAGAACAATGGATGGGC+AGG	0.514058	7.2:+25739215	MS.gene033474:CDS
TCAGATTTATGGTCGAAGAC+AGG	0.518932	7.2:+25739040	MS.gene033474:CDS
CCCATGAAAGCCAACAAGAA+AGG	0.519736	7.2:+25739317	MS.gene033474:CDS
CTTCAACGTAACTATAGGTC+CGG	0.524498	7.2:-25739069	None:intergenic
GCTGGGACAAGGATCGGTGA+TGG	0.524649	7.2:+25739371	MS.gene033474:CDS
AACACCTTCAACGTAACTAT+AGG	0.525623	7.2:-25739074	None:intergenic
GAAGAGTGCTCGTTGTGGAT+GGG	0.527267	7.2:+25739144	MS.gene033474:CDS
CGCATCACACACTTCGGCAG+TGG	0.539341	7.2:-25738967	None:intergenic
ACTATTGCTGGGACAAGGAT+CGG	0.546867	7.2:+25739365	MS.gene033474:CDS
TGCAAGCACAGAACAATGGA+TGG	0.553793	7.2:+25739210	MS.gene033474:CDS
CCATAACTATTGCTGGGACA+AGG	0.560406	7.2:+25739360	MS.gene033474:CDS
CTTCAAGGAACTCCCGAACC+AGG	0.562244	7.2:-25739109	None:intergenic
TGGGCAGGTATCATTGTGAA+TGG	0.567768	7.2:+25739230	MS.gene033474:CDS
AAAGCCAACAAGAAAGGTAT+GGG	0.570606	7.2:+25739323	MS.gene033474:CDS
TTCCTTGAAGAGAAAGGCAA+TGG	0.581451	7.2:+25739122	MS.gene033474:CDS
ATGTTCCCATAACTATTGCT+GGG	0.588122	7.2:+25739354	MS.gene033474:CDS
AGGGTTCGCATCACACACTT+CGG	0.594074	7.2:-25738973	None:intergenic
TGGCTTTATGCAGACACCGA+TGG	0.594724	7.2:+25739398	MS.gene033474:CDS
TGGGACAAGGATCGGTGATG+GGG	0.608225	7.2:+25739373	MS.gene033474:CDS
AGAGTGCTCGTTGTGGATGG+GGG	0.612554	7.2:+25739146	MS.gene033474:CDS
GGTGTTTGAAGACAATGTCC+TGG	0.615251	7.2:+25739091	MS.gene033474:CDS
AGAGATGTGGATGAGATCAA+TGG	0.626789	7.2:+25739260	MS.gene033474:CDS
TCGAGAGATCAGAATTCCAT+CGG	0.636216	7.2:-25739414	None:intergenic
GTAGTGCAAGCACAGAACAA+TGG	0.640217	7.2:+25739206	MS.gene033474:CDS
GCAAGCACAGAACAATGGAT+GGG	0.641265	7.2:+25739211	MS.gene033474:CDS
CACTTCGGCAGTGGTAACCA+AGG	0.648262	7.2:-25738958	None:intergenic
GAATGGATGTATAAGAGATG+TGG	0.656133	7.2:+25739247	MS.gene033474:CDS
GATCAATGGCTGTGATATTG+GGG	0.663718	7.2:+25739274	MS.gene033474:CDS
CAATGGAAGAGTGCTCGTTG+TGG	0.668797	7.2:+25739139	MS.gene033474:CDS
TTGCGCTGTGCAATATTGGG+TGG	0.699986	7.2:+25739176	MS.gene033474:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATCTGAAAAATTGGCTGA+AGG	-	chr7.2:25739028-25739047	None:intergenic	30.0%
	AATCTGAAAAATTGGCTGAA+GGG	-	chr7.2:25739027-25739046	None:intergenic	30.0%
	CGACCATAAATCTGAAAAAT+TGG	-	chr7.2:25739035-25739054	None:intergenic	30.0%
!	AACCCTCAATTAATTTTGAG+TGG	+	chr7.2:25738990-25739009	MS.gene033474:CDS	30.0%
!!	ACCCTCAATTAATTTTGAGT+GGG	+	chr7.2:25738991-25739010	MS.gene033474:CDS	30.0%
!!	CAGCCAATTTTTCAGATTTA+TGG	+	chr7.2:25739029-25739048	MS.gene033474:CDS	30.0%
	AACACCTTCAACGTAACTAT+AGG	-	chr7.2:25739077-25739096	None:intergenic	35.0%
	AGATCAATGGCTGTGATATT+GGG	+	chr7.2:25739273-25739292	MS.gene033474:CDS	35.0%
	ATGTTCCCATAACTATTGCT+GGG	+	chr7.2:25739354-25739373	MS.gene033474:CDS	35.0%
	CCCCACTCAAAATTAATTGA+GGG	-	chr7.2:25738995-25739014	None:intergenic	35.0%
	GAATGGATGTATAAGAGATG+TGG	+	chr7.2:25739247-25739266	MS.gene033474:CDS	35.0%
	TCCCCACTCAAAATTAATTG+AGG	-	chr7.2:25738996-25739015	None:intergenic	35.0%
!	AAAGCCAACAAGAAAGGTAT+GGG	+	chr7.2:25739323-25739342	MS.gene033474:CDS	35.0%
!!	CCCTCAATTAATTTTGAGTG+GGG	+	chr7.2:25738992-25739011	MS.gene033474:CDS	35.0%
	AGAGATGTGGATGAGATCAA+TGG	+	chr7.2:25739260-25739279	MS.gene033474:CDS	40.0%
	AGTTTGCGCTGTGCAATATT+GGG	+	chr7.2:25739173-25739192	MS.gene033474:CDS	40.0%
	CATGTTCCCATAACTATTGC+TGG	+	chr7.2:25739353-25739372	MS.gene033474:CDS	40.0%
	CTTCAACGTAACTATAGGTC+CGG	-	chr7.2:25739072-25739091	None:intergenic	40.0%
	CTTGTCCCAGCAATAGTTAT+GGG	-	chr7.2:25739362-25739381	None:intergenic	40.0%
	GAAAGCCAACAAGAAAGGTA+TGG	+	chr7.2:25739322-25739341	MS.gene033474:CDS	40.0%
	GAGATCAATGGCTGTGATAT+TGG	+	chr7.2:25739272-25739291	MS.gene033474:CDS	40.0%
	GATCAATGGCTGTGATATTG+GGG	+	chr7.2:25739274-25739293	MS.gene033474:CDS	40.0%
	TCAGATTTATGGTCGAAGAC+AGG	+	chr7.2:25739040-25739059	MS.gene033474:CDS	40.0%
	TCGAGAGATCAGAATTCCAT+CGG	-	chr7.2:25739417-25739436	None:intergenic	40.0%
	TTCCATTGCCTTTCTCTTCA+AGG	-	chr7.2:25739127-25739146	None:intergenic	40.0%
	TTCCTTGAAGAGAAAGGCAA+TGG	+	chr7.2:25739122-25739141	MS.gene033474:CDS	40.0%
	TTCTCCCATACCTTTCTTGT+TGG	-	chr7.2:25739330-25739349	None:intergenic	40.0%
	TTGAAGACAATGTCCTGGTT+CGG	+	chr7.2:25739096-25739115	MS.gene033474:CDS	40.0%
!!	ACCTTTCTTGTTGGCTTTCA+TGG	-	chr7.2:25739321-25739340	None:intergenic	40.0%
!!	CCTTTCTTGTTGGCTTTCAT+GGG	-	chr7.2:25739320-25739339	None:intergenic	40.0%
	ACTATTGCTGGGACAAGGAT+CGG	+	chr7.2:25739365-25739384	MS.gene033474:CDS	45.0%
	CAATATTGCACAGCGCAAAC+TGG	-	chr7.2:25739174-25739193	None:intergenic	45.0%
	CAGTTTGCGCTGTGCAATAT+TGG	+	chr7.2:25739172-25739191	MS.gene033474:CDS	45.0%
	CCATAACTATTGCTGGGACA+AGG	+	chr7.2:25739360-25739379	MS.gene033474:CDS	45.0%
	CCCATGAAAGCCAACAAGAA+AGG	+	chr7.2:25739317-25739336	MS.gene033474:CDS	45.0%
	CCTTGTCCCAGCAATAGTTA+TGG	-	chr7.2:25739363-25739382	None:intergenic	45.0%
	CGGACCTATAGTTACGTTGA+AGG	+	chr7.2:25739070-25739089	MS.gene033474:CDS	45.0%
	GCAAGCACAGAACAATGGAT+GGG	+	chr7.2:25739211-25739230	MS.gene033474:CDS	45.0%
	GGTGTTTGAAGACAATGTCC+TGG	+	chr7.2:25739091-25739110	MS.gene033474:CDS	45.0%
	GTAGTGCAAGCACAGAACAA+TGG	+	chr7.2:25739206-25739225	MS.gene033474:CDS	45.0%
	TGAAGACAATGTCCTGGTTC+GGG	+	chr7.2:25739097-25739116	MS.gene033474:CDS	45.0%
	TGCAAGCACAGAACAATGGA+TGG	+	chr7.2:25739210-25739229	MS.gene033474:CDS	45.0%
	TGGGCAGGTATCATTGTGAA+TGG	+	chr7.2:25739230-25739249	MS.gene033474:CDS	45.0%
	TTGTTCTGTGCTTGCACTAC+AGG	-	chr7.2:25739206-25739225	None:intergenic	45.0%
	AGGGTTCGCATCACACACTT+CGG	-	chr7.2:25738976-25738995	None:intergenic	50.0%
	CGGGAGTTCCTTGAAGAGAA+AGG	+	chr7.2:25739116-25739135	MS.gene033474:CDS	50.0%
	GATATTGGGGTGAGAGCTCT+TGG	+	chr7.2:25739287-25739306	MS.gene033474:CDS	50.0%
	TTGCGCTGTGCAATATTGGG+TGG	+	chr7.2:25739176-25739195	MS.gene033474:CDS	50.0%
!	GGTCGAAGACAGGTCTTTTC+CGG	+	chr7.2:25739050-25739069	MS.gene033474:CDS	50.0%
!!	AAGAGTGCTCGTTGTGGATG+GGG	+	chr7.2:25739145-25739164	MS.gene033474:CDS	50.0%
!!	CAATGGAAGAGTGCTCGTTG+TGG	+	chr7.2:25739139-25739158	MS.gene033474:CDS	50.0%
!!	GAAGAGTGCTCGTTGTGGAT+GGG	+	chr7.2:25739144-25739163	MS.gene033474:CDS	50.0%
!!	TGGCTTTATGCAGACACCGA+TGG	+	chr7.2:25739398-25739417	MS.gene033474:CDS	50.0%
	CACTTCGGCAGTGGTAACCA+AGG	-	chr7.2:25738961-25738980	None:intergenic	55.0%
	CTGGGACAAGGATCGGTGAT+GGG	+	chr7.2:25739372-25739391	MS.gene033474:CDS	55.0%
	CTTCAAGGAACTCCCGAACC+AGG	-	chr7.2:25739112-25739131	None:intergenic	55.0%
	GCACAGAACAATGGATGGGC+AGG	+	chr7.2:25739215-25739234	MS.gene033474:CDS	55.0%
	TGGGACAAGGATCGGTGATG+GGG	+	chr7.2:25739373-25739392	MS.gene033474:CDS	55.0%
!	AGAGTGCTCGTTGTGGATGG+GGG	+	chr7.2:25739146-25739165	MS.gene033474:CDS	55.0%
!!	CAAGGATCGGTGATGGGGAA+TGG	+	chr7.2:25739378-25739397	MS.gene033474:CDS	55.0%
!!	GGAAGAGTGCTCGTTGTGGA+TGG	+	chr7.2:25739143-25739162	MS.gene033474:CDS	55.0%
	CGCATCACACACTTCGGCAG+TGG	-	chr7.2:25738970-25738989	None:intergenic	60.0%
	GCTGGGACAAGGATCGGTGA+TGG	+	chr7.2:25739371-25739390	MS.gene033474:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	25738954	25739454	25738954	ID=MS.gene033474
chr7.2	mRNA	25738954	25739454	25738954	ID=MS.gene033474.t1;Parent=MS.gene033474
chr7.2	exon	25738954	25739454	25738954	ID=MS.gene033474.t1.exon1;Parent=MS.gene033474.t1
chr7.2	CDS	25738954	25739454	25738954	ID=cds.MS.gene033474.t1;Parent=MS.gene033474.t1

Gene Sequence

>MS.gene033474

ATGGCCTTGGTTACCACTGCCGAAGTGTGTGATGCGAACCCTCAATTAATTTTGAGTGGGGAGCTTCGTGCCCTTCAGCCAATTTTTCAGATTTATGGTCGAAGACAGGTCTTTTCCGGACCTATAGTTACGTTGAAGGTGTTTGAAGACAATGTCCTGGTTCGGGAGTTCCTTGAAGAGAAAGGCAATGGAAGAGTGCTCGTTGTGGATGGGGGTGCCAGTTTGCGCTGTGCAATATTGGGTGGTAATCCTGTAGTGCAAGCACAGAACAATGGATGGGCAGGTATCATTGTGAATGGATGTATAAGAGATGTGGATGAGATCAATGGCTGTGATATTGGGGTGAGAGCTCTTGGTTCTCATCCCATGAAAGCCAACAAGAAAGGTATGGGAGAAAAACATGTTCCCATAACTATTGCTGGGACAAGGATCGGTGATGGGGAATGGCTTTATGCAGACACCGATGGAATTCTGATCTCTCGAACAGAGCTTTCTGTTTGA

Protein sequence

>MS.gene033474.t1

MALVTTAEVCDANPQLILSGELRALQPIFQIYGRRQVFSGPIVTLKVFEDNVLVREFLEEKGNGRVLVVDGGASLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALGSHPMKANKKGMGEKHVPITIAGTRIGDGEWLYADTDGILISRTELSV