Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52579.t1 | XP_003624176.1 | 100 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.80E-89 | 337.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52579.t1 | Q9FFE0 | 83.7 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 3.0e-79 | 295.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52579.t1 | B7FGQ6 | 100.0 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.0e-89 | 337.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049585 | MS.gene52579 | 0.809918 | 1.49E-50 | -1.69E-46 |
| MS.gene04984 | MS.gene52579 | 0.823773 | 1.16E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene52579 | 0.817557 | 3.10E-52 | -1.69E-46 |
| MS.gene050826 | MS.gene52579 | 0.820232 | 7.65E-53 | -1.69E-46 |
| MS.gene051490 | MS.gene52579 | 0.830334 | 3.11E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene52579 | 0.817057 | 4.02E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene52579 | 0.804314 | 2.29E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene52579 | 0.812791 | 3.55E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene52579 | 0.813796 | 2.14E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene52579 | 0.814014 | 1.91E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene52579 | 0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene52579 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene059439 | MS.gene52579 | 0.806055 | 9.91E-50 | -1.69E-46 |
| MS.gene059713 | MS.gene52579 | 0.817029 | 4.08E-52 | -1.69E-46 |
| MS.gene060167 | MS.gene52579 | 0.823894 | 1.08E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene52579 | 0.808708 | 2.71E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene52579 | 0.836714 | 7.96E-57 | -1.69E-46 |
| MS.gene060998 | MS.gene52579 | 0.842393 | 2.66E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene52579 | MS.gene002309 | PPI |
| MS.gene024038 | MS.gene52579 | PPI |
| MS.gene52579 | MS.gene01288 | PPI |
| MS.gene51161 | MS.gene52579 | PPI |
| MS.gene02320 | MS.gene52579 | PPI |
| MS.gene033474 | MS.gene52579 | PPI |
| MS.gene52579 | MS.gene01699 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52579.t1 | MTR_7g080040 | 100.000 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.09e-120 | 337 |
| MS.gene52579.t1 | MTR_2g010815 | 66.265 | 166 | 56 | 0 | 1 | 166 | 1 | 166 | 1.22e-80 | 236 |
| MS.gene52579.t1 | MTR_2g010800 | 66.265 | 166 | 56 | 0 | 1 | 166 | 1 | 166 | 2.84e-79 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52579.t1 | AT5G16450 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 5.13e-103 | 293 |
| MS.gene52579.t1 | AT5G16450 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 5.13e-103 | 293 |
| MS.gene52579.t1 | AT3G02770 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 1.41e-102 | 291 |
| MS.gene52579.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene52579.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene52579.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene52579.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 33 | 198 | 3.70e-85 | 249 |
| MS.gene52579.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 33 | 198 | 3.70e-85 | 249 |
Find 53 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCCCATACCTTTCTTAT+TGG | 0.164687 | 7.4:-26091906 | None:intergenic |
| ACCTTTCTTATTGGCTTTCA+TGG | 0.257848 | 7.4:-26091897 | None:intergenic |
| GAGATCAATGGTTGTGATAT+TGG | 0.289628 | 7.4:+26091851 | MS.gene52579:CDS |
| AGATCAATGGTTGTGATATT+GGG | 0.305246 | 7.4:+26091852 | MS.gene52579:CDS |
| CAGTTTGCGCTGTGCAATAT+TGG | 0.315099 | 7.4:+26091751 | MS.gene52579:CDS |
| TTCCATTGCCTTTCTCTTCA+AGG | 0.326061 | 7.4:-26091703 | None:intergenic |
| TTGTTCTGTGCTTGCACTAC+AGG | 0.344974 | 7.4:-26091782 | None:intergenic |
| CATGTTCCCATAACTATTGC+TGG | 0.375703 | 7.4:+26091932 | MS.gene52579:CDS |
| TGAAGACAATGTCCTGGTTC+GGG | 0.379961 | 7.4:+26091676 | MS.gene52579:CDS |
| AGTTTGCGCTGTGCAATATT+GGG | 0.401915 | 7.4:+26091752 | MS.gene52579:CDS |
| CAATATTGCACAGCGCAAAC+TGG | 0.407075 | 7.4:-26091750 | None:intergenic |
| GATATTGGGGTGAGAGCTCT+TGG | 0.417624 | 7.4:+26091866 | MS.gene52579:CDS |
| TTGAAGACAATGTCCTGGTT+CGG | 0.418613 | 7.4:+26091675 | MS.gene52579:CDS |
| CCTTGTCCCAGCAATAGTTA+TGG | 0.430126 | 7.4:-26091939 | None:intergenic |
| CTTGTCCCAGCAATAGTTAT+GGG | 0.433835 | 7.4:-26091938 | None:intergenic |
| CCTTTCTTATTGGCTTTCAT+GGG | 0.438890 | 7.4:-26091896 | None:intergenic |
| CGGACCTATAGTTACGTTGA+AGG | 0.453624 | 7.4:+26091649 | MS.gene52579:CDS |
| GAAAGCCAATAAGAAAGGTA+TGG | 0.467226 | 7.4:+26091901 | MS.gene52579:CDS |
| CAAGGATCGGTGATGGGGAA+TGG | 0.483479 | 7.4:+26091957 | MS.gene52579:CDS |
| CTGGGACAAGGATCGGTGAT+GGG | 0.483554 | 7.4:+26091951 | MS.gene52579:CDS |
| CGGGAGTTCCTTGAAGAGAA+AGG | 0.485386 | 7.4:+26091695 | MS.gene52579:CDS |
| AAGAGTGCTCGTTGTGGATG+GGG | 0.493016 | 7.4:+26091724 | MS.gene52579:CDS |
| TCCTTGAAATAAAATGGCTC+TGG | 0.495526 | 7.4:+26091520 | None:intergenic |
| GGAAGAGTGCTCGTTGTGGA+TGG | 0.499526 | 7.4:+26091722 | MS.gene52579:CDS |
| GCACAGAACAATGGATGGGC+AGG | 0.514058 | 7.4:+26091794 | MS.gene52579:CDS |
| CCCATGAAAGCCAATAAGAA+AGG | 0.521845 | 7.4:+26091896 | MS.gene52579:CDS |
| CTTCAACGTAACTATAGGTC+CGG | 0.524498 | 7.4:-26091648 | None:intergenic |
| GCTGGGACAAGGATCGGTGA+TGG | 0.524649 | 7.4:+26091950 | MS.gene52579:CDS |
| AACACCTTCAACGTAACTAT+AGG | 0.525623 | 7.4:-26091653 | None:intergenic |
| GAAGAGTGCTCGTTGTGGAT+GGG | 0.527267 | 7.4:+26091723 | MS.gene52579:CDS |
| ACTATTGCTGGGACAAGGAT+CGG | 0.546867 | 7.4:+26091944 | MS.gene52579:CDS |
| AAAGCCAATAAGAAAGGTAT+GGG | 0.553213 | 7.4:+26091902 | MS.gene52579:CDS |
| TGCAAGCACAGAACAATGGA+TGG | 0.553793 | 7.4:+26091789 | MS.gene52579:CDS |
| CCATAACTATTGCTGGGACA+AGG | 0.560406 | 7.4:+26091939 | MS.gene52579:CDS |
| CTTCAAGGAACTCCCGAACC+AGG | 0.562244 | 7.4:-26091688 | None:intergenic |
| TGGGCAGGTATCATTGTGAA+TGG | 0.567768 | 7.4:+26091809 | MS.gene52579:CDS |
| TTCCTTGAAGAGAAAGGCAA+TGG | 0.581451 | 7.4:+26091701 | MS.gene52579:CDS |
| ATGTTCCCATAACTATTGCT+GGG | 0.588122 | 7.4:+26091933 | MS.gene52579:CDS |
| TGGCTTTATGCAGACACCGA+TGG | 0.594724 | 7.4:+26091977 | MS.gene52579:CDS |
| CCCCGCTCAAAATTAACTGA+GGG | 0.606816 | 7.4:-26091571 | None:intergenic |
| TGGGACAAGGATCGGTGATG+GGG | 0.608225 | 7.4:+26091952 | MS.gene52579:CDS |
| AGAGTGCTCGTTGTGGATGG+GGG | 0.612554 | 7.4:+26091725 | MS.gene52579:CDS |
| GGTGTTTGAAGACAATGTCC+TGG | 0.615251 | 7.4:+26091670 | MS.gene52579:CDS |
| AGGGTTCGCATCACACACTT+CGG | 0.620165 | 7.4:-26091552 | None:intergenic |
| AGAGATGTGGATGAGATCAA+TGG | 0.633740 | 7.4:+26091839 | MS.gene52579:CDS |
| GATCAATGGTTGTGATATTG+GGG | 0.635484 | 7.4:+26091853 | MS.gene52579:CDS |
| TCGAGAGATCAGAATTCCAT+CGG | 0.636216 | 7.4:-26091993 | None:intergenic |
| GTAGTGCAAGCACAGAACAA+TGG | 0.640217 | 7.4:+26091785 | MS.gene52579:CDS |
| TCCCCGCTCAAAATTAACTG+AGG | 0.641261 | 7.4:-26091572 | None:intergenic |
| GCAAGCACAGAACAATGGAT+GGG | 0.641265 | 7.4:+26091790 | MS.gene52579:CDS |
| GAATGGATGTATAAGAGATG+TGG | 0.656133 | 7.4:+26091826 | MS.gene52579:CDS |
| CAATGGAAGAGTGCTCGTTG+TGG | 0.668797 | 7.4:+26091718 | MS.gene52579:CDS |
| TTGCGCTGTGCAATATTGGG+TGG | 0.699986 | 7.4:+26091755 | MS.gene52579:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCTGAAAAATTGGTTGA+AGG | - | chr7.4:26091607-26091626 | None:intergenic | 25.0% |
| ! | AATCTGAAAAATTGGTTGAA+GGG | - | chr7.4:26091606-26091625 | None:intergenic | 25.0% |
| !!! | CAACCAATTTTTCAGATTTA+TGG | + | chr7.4:26091608-26091627 | MS.gene52579:CDS | 25.0% |
| AGATCAATGGTTGTGATATT+GGG | + | chr7.4:26091852-26091871 | MS.gene52579:CDS | 30.0% | |
| CGACCATAAATCTGAAAAAT+TGG | - | chr7.4:26091614-26091633 | None:intergenic | 30.0% | |
| ! | AAAGCCAATAAGAAAGGTAT+GGG | + | chr7.4:26091902-26091921 | MS.gene52579:CDS | 30.0% |
| AACACCTTCAACGTAACTAT+AGG | - | chr7.4:26091656-26091675 | None:intergenic | 35.0% | |
| ATGTTCCCATAACTATTGCT+GGG | + | chr7.4:26091933-26091952 | MS.gene52579:CDS | 35.0% | |
| GAAAGCCAATAAGAAAGGTA+TGG | + | chr7.4:26091901-26091920 | MS.gene52579:CDS | 35.0% | |
| GAATGGATGTATAAGAGATG+TGG | + | chr7.4:26091826-26091845 | MS.gene52579:CDS | 35.0% | |
| GAGATCAATGGTTGTGATAT+TGG | + | chr7.4:26091851-26091870 | MS.gene52579:CDS | 35.0% | |
| GATCAATGGTTGTGATATTG+GGG | + | chr7.4:26091853-26091872 | MS.gene52579:CDS | 35.0% | |
| TTCTCCCATACCTTTCTTAT+TGG | - | chr7.4:26091909-26091928 | None:intergenic | 35.0% | |
| ! | AACCCTCAGTTAATTTTGAG+CGG | + | chr7.4:26091569-26091588 | MS.gene52579:CDS | 35.0% |
| !! | ACCTTTCTTATTGGCTTTCA+TGG | - | chr7.4:26091900-26091919 | None:intergenic | 35.0% |
| !! | CCTTTCTTATTGGCTTTCAT+GGG | - | chr7.4:26091899-26091918 | None:intergenic | 35.0% |
| AGAGATGTGGATGAGATCAA+TGG | + | chr7.4:26091839-26091858 | MS.gene52579:CDS | 40.0% | |
| AGTTTGCGCTGTGCAATATT+GGG | + | chr7.4:26091752-26091771 | MS.gene52579:CDS | 40.0% | |
| CATGTTCCCATAACTATTGC+TGG | + | chr7.4:26091932-26091951 | MS.gene52579:CDS | 40.0% | |
| CCCATGAAAGCCAATAAGAA+AGG | + | chr7.4:26091896-26091915 | MS.gene52579:CDS | 40.0% | |
| CTTCAACGTAACTATAGGTC+CGG | - | chr7.4:26091651-26091670 | None:intergenic | 40.0% | |
| CTTGTCCCAGCAATAGTTAT+GGG | - | chr7.4:26091941-26091960 | None:intergenic | 40.0% | |
| TCGAGAGATCAGAATTCCAT+CGG | - | chr7.4:26091996-26092015 | None:intergenic | 40.0% | |
| TTCCATTGCCTTTCTCTTCA+AGG | - | chr7.4:26091706-26091725 | None:intergenic | 40.0% | |
| TTCCTTGAAGAGAAAGGCAA+TGG | + | chr7.4:26091701-26091720 | MS.gene52579:CDS | 40.0% | |
| TTGAAGACAATGTCCTGGTT+CGG | + | chr7.4:26091675-26091694 | MS.gene52579:CDS | 40.0% | |
| ! | ACCCTCAGTTAATTTTGAGC+GGG | + | chr7.4:26091570-26091589 | MS.gene52579:CDS | 40.0% |
| ACTATTGCTGGGACAAGGAT+CGG | + | chr7.4:26091944-26091963 | MS.gene52579:CDS | 45.0% | |
| CAATATTGCACAGCGCAAAC+TGG | - | chr7.4:26091753-26091772 | None:intergenic | 45.0% | |
| CAGTTTGCGCTGTGCAATAT+TGG | + | chr7.4:26091751-26091770 | MS.gene52579:CDS | 45.0% | |
| CCATAACTATTGCTGGGACA+AGG | + | chr7.4:26091939-26091958 | MS.gene52579:CDS | 45.0% | |
| CCCCGCTCAAAATTAACTGA+GGG | - | chr7.4:26091574-26091593 | None:intergenic | 45.0% | |
| CCTTGTCCCAGCAATAGTTA+TGG | - | chr7.4:26091942-26091961 | None:intergenic | 45.0% | |
| CGGACCTATAGTTACGTTGA+AGG | + | chr7.4:26091649-26091668 | MS.gene52579:CDS | 45.0% | |
| GCAAGCACAGAACAATGGAT+GGG | + | chr7.4:26091790-26091809 | MS.gene52579:CDS | 45.0% | |
| GGTGTTTGAAGACAATGTCC+TGG | + | chr7.4:26091670-26091689 | MS.gene52579:CDS | 45.0% | |
| GTAGTGCAAGCACAGAACAA+TGG | + | chr7.4:26091785-26091804 | MS.gene52579:CDS | 45.0% | |
| TCCCCGCTCAAAATTAACTG+AGG | - | chr7.4:26091575-26091594 | None:intergenic | 45.0% | |
| TGAAGACAATGTCCTGGTTC+GGG | + | chr7.4:26091676-26091695 | MS.gene52579:CDS | 45.0% | |
| TGCAAGCACAGAACAATGGA+TGG | + | chr7.4:26091789-26091808 | MS.gene52579:CDS | 45.0% | |
| TGGGCAGGTATCATTGTGAA+TGG | + | chr7.4:26091809-26091828 | MS.gene52579:CDS | 45.0% | |
| TTGTTCTGTGCTTGCACTAC+AGG | - | chr7.4:26091785-26091804 | None:intergenic | 45.0% | |
| ! | CCCTCAGTTAATTTTGAGCG+GGG | + | chr7.4:26091571-26091590 | MS.gene52579:CDS | 45.0% |
| ! | GGTCGAAGACAAGTCTTTTC+CGG | + | chr7.4:26091629-26091648 | MS.gene52579:CDS | 45.0% |
| AGGGTTCGCATCACACACTT+CGG | - | chr7.4:26091555-26091574 | None:intergenic | 50.0% | |
| CGGGAGTTCCTTGAAGAGAA+AGG | + | chr7.4:26091695-26091714 | MS.gene52579:CDS | 50.0% | |
| GATATTGGGGTGAGAGCTCT+TGG | + | chr7.4:26091866-26091885 | MS.gene52579:CDS | 50.0% | |
| TTGCGCTGTGCAATATTGGG+TGG | + | chr7.4:26091755-26091774 | MS.gene52579:CDS | 50.0% | |
| !! | AAGAGTGCTCGTTGTGGATG+GGG | + | chr7.4:26091724-26091743 | MS.gene52579:CDS | 50.0% |
| !! | CAATGGAAGAGTGCTCGTTG+TGG | + | chr7.4:26091718-26091737 | MS.gene52579:CDS | 50.0% |
| !! | GAAGAGTGCTCGTTGTGGAT+GGG | + | chr7.4:26091723-26091742 | MS.gene52579:CDS | 50.0% |
| !! | TGGCTTTATGCAGACACCGA+TGG | + | chr7.4:26091977-26091996 | MS.gene52579:CDS | 50.0% |
| CTGGGACAAGGATCGGTGAT+GGG | + | chr7.4:26091951-26091970 | MS.gene52579:CDS | 55.0% | |
| CTTCAAGGAACTCCCGAACC+AGG | - | chr7.4:26091691-26091710 | None:intergenic | 55.0% | |
| GCACAGAACAATGGATGGGC+AGG | + | chr7.4:26091794-26091813 | MS.gene52579:CDS | 55.0% | |
| TGGGACAAGGATCGGTGATG+GGG | + | chr7.4:26091952-26091971 | MS.gene52579:CDS | 55.0% | |
| ! | AGAGTGCTCGTTGTGGATGG+GGG | + | chr7.4:26091725-26091744 | MS.gene52579:CDS | 55.0% |
| !! | CAAGGATCGGTGATGGGGAA+TGG | + | chr7.4:26091957-26091976 | MS.gene52579:CDS | 55.0% |
| !! | GGAAGAGTGCTCGTTGTGGA+TGG | + | chr7.4:26091722-26091741 | MS.gene52579:CDS | 55.0% |
| GCTGGGACAAGGATCGGTGA+TGG | + | chr7.4:26091950-26091969 | MS.gene52579:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26091533 | 26092033 | 26091533 | ID=MS.gene52579 |
| chr7.4 | mRNA | 26091533 | 26092033 | 26091533 | ID=MS.gene52579.t1;Parent=MS.gene52579 |
| chr7.4 | exon | 26091533 | 26092033 | 26091533 | ID=MS.gene52579.t1.exon1;Parent=MS.gene52579.t1 |
| chr7.4 | CDS | 26091533 | 26092033 | 26091533 | ID=cds.MS.gene52579.t1;Parent=MS.gene52579.t1 |
| Gene Sequence |
| Protein sequence |
