Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51161.t1 | XP_003624176.1 | 100 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.80E-89 | 337.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51161.t1 | Q9FFE0 | 83.7 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 3.0e-79 | 295.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51161.t1 | B7FGQ6 | 100.0 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.0e-89 | 337.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene51161 | 0.827295 | 1.69E-54 | -1.69E-46 |
| MS.gene04917 | MS.gene51161 | 0.827464 | 1.54E-54 | -1.69E-46 |
| MS.gene04984 | MS.gene51161 | 0.818418 | 1.98E-52 | -1.69E-46 |
| MS.gene050114 | MS.gene51161 | 0.810259 | 1.26E-50 | -1.69E-46 |
| MS.gene050288 | MS.gene51161 | 0.829749 | 4.32E-55 | -1.69E-46 |
| MS.gene050317 | MS.gene51161 | 0.820383 | 7.06E-53 | -1.69E-46 |
| MS.gene050320 | MS.gene51161 | 0.80596 | 1.04E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene51161 | 0.807901 | 4.03E-50 | -1.69E-46 |
| MS.gene056752 | MS.gene51161 | 0.819859 | 9.31E-53 | -1.69E-46 |
| MS.gene056849 | MS.gene51161 | 0.806639 | 7.46E-50 | -1.69E-46 |
| MS.gene056975 | MS.gene51161 | 0.820876 | 5.44E-53 | -1.69E-46 |
| MS.gene058758 | MS.gene51161 | 0.804496 | 2.10E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene51161 | 0.840247 | 9.76E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51161 | MS.gene033474 | PPI |
| MS.gene51161 | MS.gene01288 | PPI |
| MS.gene51161 | MS.gene024038 | PPI |
| MS.gene51161 | MS.gene01699 | PPI |
| MS.gene51161 | MS.gene002309 | PPI |
| MS.gene51161 | MS.gene52579 | PPI |
| MS.gene51161 | MS.gene02320 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51161.t1 | MTR_7g080040 | 100.000 | 166 | 0 | 0 | 1 | 166 | 1 | 166 | 2.09e-120 | 337 |
| MS.gene51161.t1 | MTR_2g010815 | 66.265 | 166 | 56 | 0 | 1 | 166 | 1 | 166 | 1.22e-80 | 236 |
| MS.gene51161.t1 | MTR_2g010800 | 66.265 | 166 | 56 | 0 | 1 | 166 | 1 | 166 | 2.84e-79 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51161.t1 | AT5G16450 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 5.13e-103 | 293 |
| MS.gene51161.t1 | AT5G16450 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 5.13e-103 | 293 |
| MS.gene51161.t1 | AT3G02770 | 83.735 | 166 | 27 | 0 | 1 | 166 | 1 | 166 | 1.41e-102 | 291 |
| MS.gene51161.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene51161.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene51161.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 1 | 166 | 1.64e-85 | 248 |
| MS.gene51161.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 33 | 198 | 3.70e-85 | 249 |
| MS.gene51161.t1 | AT5G56260 | 71.687 | 166 | 47 | 0 | 1 | 166 | 33 | 198 | 3.70e-85 | 249 |
Find 54 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCCCATACCTTTCTTAT+TGG | 0.164687 | 7.3:-27388872 | None:intergenic |
| ACCTTTCTTATTGGCTTTCA+TGG | 0.257848 | 7.3:-27388863 | None:intergenic |
| GAGATCAATGGTTGTGATAT+TGG | 0.289628 | 7.3:+27388817 | MS.gene51161:CDS |
| AGATCAATGGTTGTGATATT+GGG | 0.305246 | 7.3:+27388818 | MS.gene51161:CDS |
| CAGTTTGCGCTGTGCAATAT+TGG | 0.315099 | 7.3:+27388717 | MS.gene51161:CDS |
| TTGTTCTGTGCTTGCACTAC+AGG | 0.344974 | 7.3:-27388748 | None:intergenic |
| CATGTTCCCATAACTATTGC+TGG | 0.375703 | 7.3:+27388898 | MS.gene51161:CDS |
| TTCCATTGCCTTTCTCTTCG+AGG | 0.375846 | 7.3:-27388669 | None:intergenic |
| TGAAGACAATGTCCTGGTTC+GGG | 0.379961 | 7.3:+27388642 | MS.gene51161:CDS |
| AGTTTGCGCTGTGCAATATT+GGG | 0.401915 | 7.3:+27388718 | MS.gene51161:CDS |
| CAATATTGCACAGCGCAAAC+TGG | 0.407075 | 7.3:-27388716 | None:intergenic |
| TTGAAGACAATGTCCTGGTT+CGG | 0.418613 | 7.3:+27388641 | MS.gene51161:CDS |
| CCTTGTCCCAGCAATAGTTA+TGG | 0.430126 | 7.3:-27388905 | None:intergenic |
| CTTGTCCCAGCAATAGTTAT+GGG | 0.433835 | 7.3:-27388904 | None:intergenic |
| CCTTTCTTATTGGCTTTCAT+GGG | 0.438890 | 7.3:-27388862 | None:intergenic |
| GATATTGGGGTGAGAGCTCT+TGG | 0.450174 | 7.3:+27388832 | MS.gene51161:CDS |
| CGGACCTATAGTTACGTTGA+AGG | 0.453624 | 7.3:+27388615 | MS.gene51161:CDS |
| GAAAGCCAATAAGAAAGGTA+TGG | 0.467226 | 7.3:+27388867 | MS.gene51161:CDS |
| CAAGGATCGGTGATGGGGAA+TGG | 0.483479 | 7.3:+27388923 | MS.gene51161:CDS |
| CTGGGACAAGGATCGGTGAT+GGG | 0.483554 | 7.3:+27388917 | MS.gene51161:CDS |
| AAGAGTGCTCGTTGTGGATG+GGG | 0.493016 | 7.3:+27388690 | MS.gene51161:CDS |
| TCCTTGAAATAAAATGGCTC+TGG | 0.495526 | 7.3:+27388486 | None:intergenic |
| GGAAGAGTGCTCGTTGTGGA+TGG | 0.499526 | 7.3:+27388688 | MS.gene51161:CDS |
| GCACAGAACAATGGATGGGC+AGG | 0.514058 | 7.3:+27388760 | MS.gene51161:CDS |
| TCAGATTTATGGTCGAAGAC+AGG | 0.518932 | 7.3:+27388585 | MS.gene51161:CDS |
| CCCATGAAAGCCAATAAGAA+AGG | 0.521845 | 7.3:+27388862 | MS.gene51161:CDS |
| CTTCAACGTAACTATAGGTC+CGG | 0.524498 | 7.3:-27388614 | None:intergenic |
| GCTGGGACAAGGATCGGTGA+TGG | 0.524649 | 7.3:+27388916 | MS.gene51161:CDS |
| AACACCTTCAACGTAACTAT+AGG | 0.525623 | 7.3:-27388619 | None:intergenic |
| GAAGAGTGCTCGTTGTGGAT+GGG | 0.527267 | 7.3:+27388689 | MS.gene51161:CDS |
| CGGGAGTTCCTCGAAGAGAA+AGG | 0.531311 | 7.3:+27388661 | MS.gene51161:CDS |
| ACTATTGCTGGGACAAGGAT+CGG | 0.546867 | 7.3:+27388910 | MS.gene51161:CDS |
| AAAGCCAATAAGAAAGGTAT+GGG | 0.553213 | 7.3:+27388868 | MS.gene51161:CDS |
| TGCAAGCACAGAACAATGGA+TGG | 0.553793 | 7.3:+27388755 | MS.gene51161:CDS |
| CCATAACTATTGCTGGGACA+AGG | 0.560406 | 7.3:+27388905 | MS.gene51161:CDS |
| CTTCGAGGAACTCCCGAACC+AGG | 0.562244 | 7.3:-27388654 | None:intergenic |
| TGGGCAGGTATCATTGTGAA+TGG | 0.567768 | 7.3:+27388775 | MS.gene51161:CDS |
| ATGTTCCCATAACTATTGCT+GGG | 0.588122 | 7.3:+27388899 | MS.gene51161:CDS |
| TGGCTTTATGCAGACACCGA+TGG | 0.594724 | 7.3:+27388943 | MS.gene51161:CDS |
| CCCCGCTCAAAATTAACTGA+GGG | 0.606816 | 7.3:-27388537 | None:intergenic |
| TTCCTCGAAGAGAAAGGCAA+TGG | 0.608108 | 7.3:+27388667 | MS.gene51161:CDS |
| TGGGACAAGGATCGGTGATG+GGG | 0.608225 | 7.3:+27388918 | MS.gene51161:CDS |
| AGAGTGCTCGTTGTGGATGG+GGG | 0.612554 | 7.3:+27388691 | MS.gene51161:CDS |
| GGTGTTTGAAGACAATGTCC+TGG | 0.615251 | 7.3:+27388636 | MS.gene51161:CDS |
| AGGGTTCGCATCACACACTT+CGG | 0.620165 | 7.3:-27388518 | None:intergenic |
| AGAGATGTGGATGAGATCAA+TGG | 0.633740 | 7.3:+27388805 | MS.gene51161:CDS |
| GATCAATGGTTGTGATATTG+GGG | 0.635484 | 7.3:+27388819 | MS.gene51161:CDS |
| TCGAGAGATCAGAATTCCAT+CGG | 0.636216 | 7.3:-27388959 | None:intergenic |
| GTAGTGCAAGCACAGAACAA+TGG | 0.640217 | 7.3:+27388751 | MS.gene51161:CDS |
| TCCCCGCTCAAAATTAACTG+AGG | 0.641261 | 7.3:-27388538 | None:intergenic |
| GCAAGCACAGAACAATGGAT+GGG | 0.641265 | 7.3:+27388756 | MS.gene51161:CDS |
| GAATGGATGTATAAGAGATG+TGG | 0.656133 | 7.3:+27388792 | MS.gene51161:CDS |
| CAATGGAAGAGTGCTCGTTG+TGG | 0.668797 | 7.3:+27388684 | MS.gene51161:CDS |
| TTGCGCTGTGCAATATTGGG+TGG | 0.699986 | 7.3:+27388721 | MS.gene51161:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCTGAAAAATTGGTTGA+AGG | - | chr7.3:27388573-27388592 | None:intergenic | 25.0% |
| ! | AATCTGAAAAATTGGTTGAA+GGG | - | chr7.3:27388572-27388591 | None:intergenic | 25.0% |
| !!! | CAACCAATTTTTCAGATTTA+TGG | + | chr7.3:27388574-27388593 | MS.gene51161:CDS | 25.0% |
| AGATCAATGGTTGTGATATT+GGG | + | chr7.3:27388818-27388837 | MS.gene51161:CDS | 30.0% | |
| CGACCATAAATCTGAAAAAT+TGG | - | chr7.3:27388580-27388599 | None:intergenic | 30.0% | |
| ! | AAAGCCAATAAGAAAGGTAT+GGG | + | chr7.3:27388868-27388887 | MS.gene51161:CDS | 30.0% |
| AACACCTTCAACGTAACTAT+AGG | - | chr7.3:27388622-27388641 | None:intergenic | 35.0% | |
| ATGTTCCCATAACTATTGCT+GGG | + | chr7.3:27388899-27388918 | MS.gene51161:CDS | 35.0% | |
| GAAAGCCAATAAGAAAGGTA+TGG | + | chr7.3:27388867-27388886 | MS.gene51161:CDS | 35.0% | |
| GAATGGATGTATAAGAGATG+TGG | + | chr7.3:27388792-27388811 | MS.gene51161:CDS | 35.0% | |
| GAGATCAATGGTTGTGATAT+TGG | + | chr7.3:27388817-27388836 | MS.gene51161:CDS | 35.0% | |
| GATCAATGGTTGTGATATTG+GGG | + | chr7.3:27388819-27388838 | MS.gene51161:CDS | 35.0% | |
| TTCTCCCATACCTTTCTTAT+TGG | - | chr7.3:27388875-27388894 | None:intergenic | 35.0% | |
| ! | AACCCTCAGTTAATTTTGAG+CGG | + | chr7.3:27388535-27388554 | MS.gene51161:CDS | 35.0% |
| !! | ACCTTTCTTATTGGCTTTCA+TGG | - | chr7.3:27388866-27388885 | None:intergenic | 35.0% |
| !! | CCTTTCTTATTGGCTTTCAT+GGG | - | chr7.3:27388865-27388884 | None:intergenic | 35.0% |
| AGAGATGTGGATGAGATCAA+TGG | + | chr7.3:27388805-27388824 | MS.gene51161:CDS | 40.0% | |
| AGTTTGCGCTGTGCAATATT+GGG | + | chr7.3:27388718-27388737 | MS.gene51161:CDS | 40.0% | |
| CATGTTCCCATAACTATTGC+TGG | + | chr7.3:27388898-27388917 | MS.gene51161:CDS | 40.0% | |
| CCCATGAAAGCCAATAAGAA+AGG | + | chr7.3:27388862-27388881 | MS.gene51161:CDS | 40.0% | |
| CTTCAACGTAACTATAGGTC+CGG | - | chr7.3:27388617-27388636 | None:intergenic | 40.0% | |
| CTTGTCCCAGCAATAGTTAT+GGG | - | chr7.3:27388907-27388926 | None:intergenic | 40.0% | |
| TCAGATTTATGGTCGAAGAC+AGG | + | chr7.3:27388585-27388604 | MS.gene51161:CDS | 40.0% | |
| TCGAGAGATCAGAATTCCAT+CGG | - | chr7.3:27388962-27388981 | None:intergenic | 40.0% | |
| TTGAAGACAATGTCCTGGTT+CGG | + | chr7.3:27388641-27388660 | MS.gene51161:CDS | 40.0% | |
| ! | ACCCTCAGTTAATTTTGAGC+GGG | + | chr7.3:27388536-27388555 | MS.gene51161:CDS | 40.0% |
| ACTATTGCTGGGACAAGGAT+CGG | + | chr7.3:27388910-27388929 | MS.gene51161:CDS | 45.0% | |
| CAATATTGCACAGCGCAAAC+TGG | - | chr7.3:27388719-27388738 | None:intergenic | 45.0% | |
| CAGTTTGCGCTGTGCAATAT+TGG | + | chr7.3:27388717-27388736 | MS.gene51161:CDS | 45.0% | |
| CCATAACTATTGCTGGGACA+AGG | + | chr7.3:27388905-27388924 | MS.gene51161:CDS | 45.0% | |
| CCCCGCTCAAAATTAACTGA+GGG | - | chr7.3:27388540-27388559 | None:intergenic | 45.0% | |
| CCTTGTCCCAGCAATAGTTA+TGG | - | chr7.3:27388908-27388927 | None:intergenic | 45.0% | |
| CGGACCTATAGTTACGTTGA+AGG | + | chr7.3:27388615-27388634 | MS.gene51161:CDS | 45.0% | |
| GCAAGCACAGAACAATGGAT+GGG | + | chr7.3:27388756-27388775 | MS.gene51161:CDS | 45.0% | |
| GGTGTTTGAAGACAATGTCC+TGG | + | chr7.3:27388636-27388655 | MS.gene51161:CDS | 45.0% | |
| GTAGTGCAAGCACAGAACAA+TGG | + | chr7.3:27388751-27388770 | MS.gene51161:CDS | 45.0% | |
| TCCCCGCTCAAAATTAACTG+AGG | - | chr7.3:27388541-27388560 | None:intergenic | 45.0% | |
| TGAAGACAATGTCCTGGTTC+GGG | + | chr7.3:27388642-27388661 | MS.gene51161:CDS | 45.0% | |
| TGCAAGCACAGAACAATGGA+TGG | + | chr7.3:27388755-27388774 | MS.gene51161:CDS | 45.0% | |
| TGGGCAGGTATCATTGTGAA+TGG | + | chr7.3:27388775-27388794 | MS.gene51161:CDS | 45.0% | |
| TTCCATTGCCTTTCTCTTCG+AGG | - | chr7.3:27388672-27388691 | None:intergenic | 45.0% | |
| TTCCTCGAAGAGAAAGGCAA+TGG | + | chr7.3:27388667-27388686 | MS.gene51161:CDS | 45.0% | |
| TTGTTCTGTGCTTGCACTAC+AGG | - | chr7.3:27388751-27388770 | None:intergenic | 45.0% | |
| ! | CCCTCAGTTAATTTTGAGCG+GGG | + | chr7.3:27388537-27388556 | MS.gene51161:CDS | 45.0% |
| AGGGTTCGCATCACACACTT+CGG | - | chr7.3:27388521-27388540 | None:intergenic | 50.0% | |
| GATATTGGGGTGAGAGCTCT+TGG | + | chr7.3:27388832-27388851 | MS.gene51161:CDS | 50.0% | |
| TTGCGCTGTGCAATATTGGG+TGG | + | chr7.3:27388721-27388740 | MS.gene51161:CDS | 50.0% | |
| ! | GGTCGAAGACAGGTCTTTTC+CGG | + | chr7.3:27388595-27388614 | MS.gene51161:CDS | 50.0% |
| !! | AAGAGTGCTCGTTGTGGATG+GGG | + | chr7.3:27388690-27388709 | MS.gene51161:CDS | 50.0% |
| !! | CAATGGAAGAGTGCTCGTTG+TGG | + | chr7.3:27388684-27388703 | MS.gene51161:CDS | 50.0% |
| !! | GAAGAGTGCTCGTTGTGGAT+GGG | + | chr7.3:27388689-27388708 | MS.gene51161:CDS | 50.0% |
| !! | TGGCTTTATGCAGACACCGA+TGG | + | chr7.3:27388943-27388962 | MS.gene51161:CDS | 50.0% |
| CGGGAGTTCCTCGAAGAGAA+AGG | + | chr7.3:27388661-27388680 | MS.gene51161:CDS | 55.0% | |
| CTGGGACAAGGATCGGTGAT+GGG | + | chr7.3:27388917-27388936 | MS.gene51161:CDS | 55.0% | |
| GCACAGAACAATGGATGGGC+AGG | + | chr7.3:27388760-27388779 | MS.gene51161:CDS | 55.0% | |
| TGGGACAAGGATCGGTGATG+GGG | + | chr7.3:27388918-27388937 | MS.gene51161:CDS | 55.0% | |
| ! | AGAGTGCTCGTTGTGGATGG+GGG | + | chr7.3:27388691-27388710 | MS.gene51161:CDS | 55.0% |
| !! | CAAGGATCGGTGATGGGGAA+TGG | + | chr7.3:27388923-27388942 | MS.gene51161:CDS | 55.0% |
| !! | GGAAGAGTGCTCGTTGTGGA+TGG | + | chr7.3:27388688-27388707 | MS.gene51161:CDS | 55.0% |
| CTTCGAGGAACTCCCGAACC+AGG | - | chr7.3:27388657-27388676 | None:intergenic | 60.0% | |
| GCTGGGACAAGGATCGGTGA+TGG | + | chr7.3:27388916-27388935 | MS.gene51161:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 27388499 | 27388999 | 27388499 | ID=MS.gene51161 |
| chr7.3 | mRNA | 27388499 | 27388999 | 27388499 | ID=MS.gene51161.t1;Parent=MS.gene51161 |
| chr7.3 | exon | 27388499 | 27388999 | 27388499 | ID=MS.gene51161.t1.exon1;Parent=MS.gene51161.t1 |
| chr7.3 | CDS | 27388499 | 27388999 | 27388499 | ID=cds.MS.gene51161.t1;Parent=MS.gene51161.t1 |
| Gene Sequence |
| Protein sequence |
