AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003631

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003631.t1 MTR_4g072210 97.959 245 5 0 1 245 29 273 0.0 498
MS.gene003631.t1 MTR_5g021980 71.308 237 66 1 4 238 36 272 1.50e-127 362
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003631.t1 AT5G45600 71.610 236 67 0 9 244 31 266 3.26e-136 384
MS.gene003631.t1 AT5G45600 70.082 244 68 1 1 244 29 267 6.66e-136 383
MS.gene003631.t1 AT2G18000 58.333 240 98 1 5 242 24 263 3.58e-104 303
MS.gene003631.t1 AT2G18000 60.345 174 67 1 5 176 24 197 3.79e-78 234

Find 54 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTGCTTCTGAGAAATTC+AGG 0.264995 4.1:-31080790 None:intergenic
TATACGGGAACGTAGCATTT+TGG 0.267574 4.1:+31079559 MS.gene003631:CDS
TTCAGGAACCATTGGCTTAA+GGG 0.287646 4.1:-31080773 None:intergenic
ATTAGTGTTCCAATAGTATA+CGG 0.306773 4.1:+31079543 MS.gene003631:CDS
ATTCAGGAACCATTGGCTTA+AGG 0.353599 4.1:-31080774 None:intergenic
GCTACGTTCCCGTATACTAT+TGG 0.356357 4.1:-31079552 None:intergenic
GTCCATCTATCAAACTTATC+TGG 0.361292 4.1:-31082993 None:intergenic
AAGGAACTGCCTTCATCATC+TGG 0.371847 4.1:-31080467 None:intergenic
GCTGGCAGCAGCTCGTCAGC+AGG 0.387535 4.1:+31080823 MS.gene003631:CDS
AGTCTTGGCATGTTCACAAC+TGG 0.396171 4.1:-31080581 None:intergenic
TACCTTCTTATCATTGTCTT+CGG 0.402788 4.1:-31078670 None:intergenic
AACAGGCTTCTTTGTGGAAA+AGG 0.415370 4.1:-31080486 None:intergenic
CACAACAACAGGCTTCTTTG+TGG 0.416649 4.1:-31080492 None:intergenic
CTGAGAAATTCAGGAACCAT+TGG 0.419880 4.1:-31080781 None:intergenic
TCATAAAATTCCACAACAAC+AGG 0.422701 4.1:-31080503 None:intergenic
GGAGGCAAGGTAAGCCCAGC+AGG 0.425533 4.1:-31080605 None:intergenic
TAATTATATGTCTATTGGTC+AGG 0.426268 4.1:-31083039 None:intergenic
CGCCAGATAAGTTTGATAGA+TGG 0.434906 4.1:+31082991 MS.gene003631:CDS
CACAAAGAAGCCTGTTGTTG+TGG 0.444280 4.1:+31080493 MS.gene003631:CDS
TGGAGCATCCAACGAGGACC+TGG 0.447185 4.1:+31079757 MS.gene003631:CDS
CGTTTACTAGCATCTTCGAT+TGG 0.501880 4.1:-31080725 None:intergenic
GTAAGCCCAGCAGGTAGTCT+TGG 0.502144 4.1:-31080596 None:intergenic
CAATCGAAGATGCTAGTAAA+CGG 0.502979 4.1:+31080726 MS.gene003631:CDS
TGAGTTATCAGAGGCAGGAT+GGG 0.507588 4.1:+31079859 MS.gene003631:CDS
AGCAGATGAACTCTTGCAGC+TGG 0.510733 4.1:+31080805 MS.gene003631:CDS
CAAGGTATCAATCACATAAG+TGG 0.515392 4.1:+31079720 MS.gene003631:intron
TTGAGTTATCAGAGGCAGGA+TGG 0.518416 4.1:+31079858 MS.gene003631:CDS
CCTGCCTCTGATAACTCAAA+AGG 0.519422 4.1:-31079854 None:intergenic
ATCCGAAGACAATGATAAGA+AGG 0.520538 4.1:+31078668 MS.gene003631:CDS
CAATAATCCCACAAGGATTG+TGG 0.525358 4.1:+31079823 MS.gene003631:CDS
GAGTTATCAGAGGCAGGATG+GGG 0.541531 4.1:+31079860 MS.gene003631:CDS
GGAGCATCCAACGAGGACCT+GGG 0.547877 4.1:+31079758 MS.gene003631:CDS
GTCTTGGCATGTTCACAACT+GGG 0.560043 4.1:-31080580 None:intergenic
GGTGCATCCACAATCCTTGT+GGG 0.565259 4.1:-31079830 None:intergenic
AAGATAATCCCTTAAGCCAA+TGG 0.568475 4.1:+31080765 MS.gene003631:CDS
AAGTGGACTATATATGTTCG+TGG 0.581227 4.1:+31079737 MS.gene003631:CDS
CAAAGTAACATACTAGGAGG+AGG 0.586112 4.1:-31080623 None:intergenic
GAGCATCCAACGAGGACCTG+GGG 0.590570 4.1:+31079759 MS.gene003631:CDS
AACATGCCAAGACTACCTGC+TGG 0.595578 4.1:+31080590 MS.gene003631:CDS
TTAGTGTTCCAATAGTATAC+GGG 0.596441 4.1:+31079544 MS.gene003631:CDS
TAACATACTAGGAGGAGGCA+AGG 0.606191 4.1:-31080618 None:intergenic
AGGTGCATCCACAATCCTTG+TGG 0.606632 4.1:-31079831 None:intergenic
CAAACGACTCACAAGTTCAC+AGG 0.610052 4.1:-31079935 None:intergenic
TATACAGATATCATCAGTTG+AGG 0.617329 4.1:+31080438 MS.gene003631:intron
ACATGCCAAGACTACCTGCT+GGG 0.629730 4.1:+31080591 MS.gene003631:CDS
AGGTTGTATCCAGATGATGA+AGG 0.632066 4.1:+31080458 MS.gene003631:CDS
GCCTCTGATAACTCAAAAGG+AGG 0.653413 4.1:-31079851 None:intergenic
CTAGTTTCAATAATCCCACA+AGG 0.653695 4.1:+31079816 MS.gene003631:CDS
GATGCTAGTAAACGGAGGAG+AGG 0.657501 4.1:+31080734 MS.gene003631:CDS
ACTGTGGAAATAAAGTGTGA+TGG 0.663057 4.1:-31079897 None:intergenic
AGCATCCAACGAGGACCTGG+GGG 0.700369 4.1:+31079760 MS.gene003631:CDS
TCGAAGATGCTAGTAAACGG+AGG 0.723176 4.1:+31080729 MS.gene003631:CDS
TGTTCGTGGAGCATCCAACG+AGG 0.768144 4.1:+31079751 MS.gene003631:CDS
GCTTGTCGCAAACATCACTG+TGG 0.771336 4.1:-31079913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGTAAAAGAAAAAATAA+AGG - chr4.1:31079116-31079135 None:intergenic 10.0%
!! TTATCATTATTAATAAAATG+CGG - chr4.1:31080207-31080226 None:intergenic 10.0%
!!! ATTTAATCTTTTAGTTATTT+AGG + chr4.1:31081596-31081615 MS.gene003631:intron 10.0%
!!! TTTTTCTTAAATAAGATTAT+TGG + chr4.1:31081261-31081280 MS.gene003631:intron 10.0%
!! AATAAATGTATTGCTTAAAT+GGG + chr4.1:31079647-31079666 MS.gene003631:intron 15.0%
!! AATAATAAAATACAAGGAAA+AGG - chr4.1:31082482-31082501 None:intergenic 15.0%
!! AATATGCTTAAATAAATTTC+TGG + chr4.1:31081507-31081526 MS.gene003631:intron 15.0%
!! ATCTATTCTAATTAATACAA+AGG - chr4.1:31081232-31081251 None:intergenic 15.0%
!! CAACTTAATAATAAAATACA+AGG - chr4.1:31082488-31082507 None:intergenic 15.0%
!! TAATAAATGTATTGCTTAAA+TGG + chr4.1:31079646-31079665 MS.gene003631:intron 15.0%
!! TTAATTAAAACAACAATTGA+AGG - chr4.1:31079625-31079644 None:intergenic 15.0%
!! TTCAAATAAATATATCATCA+TGG - chr4.1:31080914-31080933 None:intergenic 15.0%
!!! ATATTTTGAATAAAACCATT+TGG - chr4.1:31081326-31081345 None:intergenic 15.0%
!!! ATTAAAAAGTTAGTTTAACT+AGG + chr4.1:31081742-31081761 MS.gene003631:intron 15.0%
!!! TATTATTTTTTTGTAGAACT+CGG + chr4.1:31079497-31079516 MS.gene003631:intron 15.0%
!!! TTAAAAAGTTAGTTTAACTA+GGG + chr4.1:31081743-31081762 MS.gene003631:intron 15.0%
!! AAAGAAAATATTTAGGAAAG+AGG - chr4.1:31081807-31081826 None:intergenic 20.0%
!! AAAGCTTCAAATTTGAATAA+AGG - chr4.1:31078782-31078801 None:intergenic 20.0%
!! AAATTGATGAAAGATAATAG+AGG - chr4.1:31082096-31082115 None:intergenic 20.0%
!! AACATATAATCAAGAATCTT+CGG + chr4.1:31081105-31081124 MS.gene003631:intron 20.0%
!! AAGTTAATTCTATCATCAAA+AGG + chr4.1:31082588-31082607 MS.gene003631:intron 20.0%
!! AATTTGCACTGTATATTTAA+TGG + chr4.1:31081777-31081796 MS.gene003631:intron 20.0%
!! AGTTAATTCTATCATCAAAA+GGG + chr4.1:31082589-31082608 MS.gene003631:intron 20.0%
!! ATAAATGTATTGCTTAAATG+GGG + chr4.1:31079648-31079667 MS.gene003631:intron 20.0%
!! ATTATCTTTCATCAATTTAC+AGG + chr4.1:31082098-31082117 MS.gene003631:intron 20.0%
!! CAGAAATAAAAAGTAAATCA+TGG - chr4.1:31082383-31082402 None:intergenic 20.0%
!! GAAGAACAAAGAAAATATTT+AGG - chr4.1:31081814-31081833 None:intergenic 20.0%
!! TAAATCATTTAGTTACAAAC+AGG - chr4.1:31082019-31082038 None:intergenic 20.0%
!! TAATTCAATTCAATTCAATC+AGG - chr4.1:31079272-31079291 None:intergenic 20.0%
!! TGTTCTAATAATTTCTATGT+GGG + chr4.1:31081438-31081457 MS.gene003631:intron 20.0%
!! TTGTTCTAATAATTTCTATG+TGG + chr4.1:31081437-31081456 MS.gene003631:intron 20.0%
!!! AATGAATAATTTTCAAGCAT+TGG - chr4.1:31080300-31080319 None:intergenic 20.0%
!!! ATTATTTTTTTGTAGAACTC+GGG + chr4.1:31079498-31079517 MS.gene003631:intron 20.0%
!!! ATTTTGACATTCACAAAATA+TGG - chr4.1:31080381-31080400 None:intergenic 20.0%
!!! GAATTGTTCAAACAATTTTT+TGG + chr4.1:31079225-31079244 MS.gene003631:intron 20.0%
!!! GTTTTAAAAACCGTTATTTT+AGG - chr4.1:31078727-31078746 None:intergenic 20.0%
!!! TGATGTTGTTTTTGATTTAT+GGG + chr4.1:31079956-31079975 MS.gene003631:intron 20.0%
!!! TTGATGTTGTTTTTGATTTA+TGG + chr4.1:31079955-31079974 MS.gene003631:intron 20.0%
!!! TTTTACTCAAATAAGATTGT+TGG + chr4.1:31081965-31081984 MS.gene003631:intron 20.0%
! AAACATTTGTGATACTATCT+GGG + chr4.1:31078984-31079003 MS.gene003631:intron 25.0%
! AAATTGCAAAGTAACATACT+AGG - chr4.1:31080632-31080651 None:intergenic 25.0%
! AACAATTTACAGCAAGTATA+TGG + chr4.1:31080942-31080961 MS.gene003631:intron 25.0%
! AAGATGTAATAAGTATCAGT+TGG - chr4.1:31082931-31082950 None:intergenic 25.0%
! AAGTACCATATGTAAAAGTA+AGG - chr4.1:31081667-31081686 None:intergenic 25.0%
! AATAAGCTAAAAACATCGTA+AGG - chr4.1:31079040-31079059 None:intergenic 25.0%
! ACATCCATTATTATCAATTG+TGG + chr4.1:31082235-31082254 MS.gene003631:intron 25.0%
! ACGTTAATTAAACTCAAATG+TGG - chr4.1:31080683-31080702 None:intergenic 25.0%
! ATTAGTGTTCCAATAGTATA+CGG + chr4.1:31079543-31079562 MS.gene003631:CDS 25.0%
! CAATGAATAATCACAAAAGT+GGG - chr4.1:31081193-31081212 None:intergenic 25.0%
! CATCCATTATTATCAATTGT+GGG + chr4.1:31082236-31082255 MS.gene003631:intron 25.0%
! CCATTATCATCAATATATTG+TGG + chr4.1:31081408-31081427 MS.gene003631:intron 25.0%
! CGGTTAAAATCCTAAAATAA+CGG + chr4.1:31078714-31078733 MS.gene003631:intron 25.0%
! GACAATGTTTATGTTATACA+AGG + chr4.1:31079702-31079721 MS.gene003631:intron 25.0%
! GTTAATGATATGTAACAAAC+AGG - chr4.1:31080090-31080109 None:intergenic 25.0%
! GTTCTAATAATTTCTATGTG+GGG + chr4.1:31081439-31081458 MS.gene003631:intron 25.0%
! TACAGACTTCATTATTAGTA+AGG - chr4.1:31080974-31080993 None:intergenic 25.0%
! TCAATGAATAATCACAAAAG+TGG - chr4.1:31081194-31081213 None:intergenic 25.0%
! TCATCAATATATTGTGGATT+AGG + chr4.1:31081414-31081433 MS.gene003631:intron 25.0%
! TGATTATATGTTTAGAAGTG+TGG - chr4.1:31081097-31081116 None:intergenic 25.0%
! TTATTTGAGCTTATCTATTG+AGG + chr4.1:31079289-31079308 MS.gene003631:intron 25.0%
!! AGCATTTTGAATATTAAGCT+AGG - chr4.1:31082288-31082307 None:intergenic 25.0%
!! ATCATTTAAGGATTTGTTTC+TGG + chr4.1:31081382-31081401 MS.gene003631:intron 25.0%
!! CCACAATATATTGATGATAA+TGG - chr4.1:31081411-31081430 None:intergenic 25.0%
!! TCAAATTTGAAGCTTTGATT+TGG + chr4.1:31078786-31078805 MS.gene003631:intron 25.0%
!!! CTTAAATAAATTTCTGGTCT+TGG + chr4.1:31081513-31081532 MS.gene003631:intron 25.0%
!!! GAAGTACACATTTTTTTGTA+AGG - chr4.1:31081555-31081574 None:intergenic 25.0%
!!! GATGTTGTTTTTGATTTATG+GGG + chr4.1:31079957-31079976 MS.gene003631:intron 25.0%
!!! TCTTTTATTGATAATGAGCT+TGG + chr4.1:31078927-31078946 MS.gene003631:intron 25.0%
!!! TTTAAAACCATACCCTTTTT+TGG + chr4.1:31078739-31078758 MS.gene003631:intron 25.0%
AACAGTGAATAATAGACCTA+TGG - chr4.1:31080337-31080356 None:intergenic 30.0%
AACTCTACTAATCCCAAAAT+TGG - chr4.1:31081914-31081933 None:intergenic 30.0%
AACTTTATGCACTCCAAAAA+AGG - chr4.1:31078755-31078774 None:intergenic 30.0%
ACTTTATGCACTCCAAAAAA+GGG - chr4.1:31078754-31078773 None:intergenic 30.0%
ATCATACATGTACACATGTT+TGG + chr4.1:31080993-31081012 MS.gene003631:intron 30.0%
ATGTACAATCGACATTTCTA+GGG - chr4.1:31081029-31081048 None:intergenic 30.0%
CAAACATTTGTGATACTATC+TGG + chr4.1:31078983-31079002 MS.gene003631:intron 30.0%
CACACAAAAAAAATGTGCTA+AGG + chr4.1:31079678-31079697 MS.gene003631:intron 30.0%
GGTTAAACTACTTAGATACA+AGG + chr4.1:31082618-31082637 MS.gene003631:intron 30.0%
GTTAGATTGAGTTAGTAGTT+TGG + chr4.1:31080169-31080188 MS.gene003631:intron 30.0%
TACCATATGTAAAAGTAAGG+TGG - chr4.1:31081664-31081683 None:intergenic 30.0%
TACCTTCTTATCATTGTCTT+CGG - chr4.1:31078673-31078692 None:intergenic 30.0%
TATACAGATATCATCAGTTG+AGG + chr4.1:31080438-31080457 MS.gene003631:intron 30.0%
TCAAAGTATGCTCAAAAGTA+GGG + chr4.1:31080040-31080059 MS.gene003631:intron 30.0%
TCATAAAATTCCACAACAAC+AGG - chr4.1:31080506-31080525 None:intergenic 30.0%
TCATGGAAACAGTATATCTT+TGG - chr4.1:31082366-31082385 None:intergenic 30.0%
TTAGTGTTCCAATAGTATAC+GGG + chr4.1:31079544-31079563 MS.gene003631:CDS 30.0%
TTCAAAGTATGCTCAAAAGT+AGG + chr4.1:31080039-31080058 MS.gene003631:intron 30.0%
TTGATATACTACATGACCAT+AGG + chr4.1:31080318-31080337 MS.gene003631:intron 30.0%
TTGTGAGTGTTCATCATTTA+AGG + chr4.1:31081370-31081389 MS.gene003631:intron 30.0%
! AACTACTTTGTTTACCCAAA+TGG - chr4.1:31082539-31082558 None:intergenic 30.0%
! CATTCTACAGATTGATTTCA+AGG - chr4.1:31078836-31078855 None:intergenic 30.0%
! CTATTGAGGTAAACACTTTT+GGG + chr4.1:31079303-31079322 MS.gene003631:intron 30.0%
! CTGTTTTCATTAAGCTATCA+TGG - chr4.1:31079398-31079417 None:intergenic 30.0%
! TCTATTGAGGTAAACACTTT+TGG + chr4.1:31079302-31079321 MS.gene003631:intron 30.0%
! TGACCCACAATTGATAATAA+TGG - chr4.1:31082242-31082261 None:intergenic 30.0%
! TGTTGTGGAATTTTATGATG+AGG + chr4.1:31080508-31080527 MS.gene003631:CDS 30.0%
!! CCTTTCTTTTGATTCAGATT+CGG + chr4.1:31078694-31078713 MS.gene003631:intron 30.0%
!! TTCCACCTTACTTTTACATA+TGG + chr4.1:31081659-31081678 MS.gene003631:intron 30.0%
!! TTTTGTTTACGTGATGCTTA+TGG + chr4.1:31081167-31081186 MS.gene003631:intron 30.0%
!!! AGCACATTTTTTTTGTGTGA+AGG - chr4.1:31079677-31079696 None:intergenic 30.0%
!!! GCTTCAACTCTATTTTTGTT+TGG + chr4.1:31081875-31081894 MS.gene003631:intron 30.0%
AAGATAATCCCTTAAGCCAA+TGG + chr4.1:31080765-31080784 MS.gene003631:CDS 35.0%
AAGTCGACGAAAATAGTGTA+TGG + chr4.1:31082688-31082707 MS.gene003631:intron 35.0%
ACTGAACATGGTCAATGATT+TGG - chr4.1:31082437-31082456 None:intergenic 35.0%
ACTGTGGAAATAAAGTGTGA+TGG - chr4.1:31079900-31079919 None:intergenic 35.0%
AGAGTTTAGCATACTGAACA+TGG - chr4.1:31082449-31082468 None:intergenic 35.0%
AGATCACAGTATCACACAAA+AGG - chr4.1:31080001-31080020 None:intergenic 35.0%
ATCCGAAGACAATGATAAGA+AGG + chr4.1:31078668-31078687 MS.gene003631:CDS 35.0%
ATGTATCTACTTCCGCAAAT+GGG - chr4.1:31082810-31082829 None:intergenic 35.0%
CAATCGAAGATGCTAGTAAA+CGG + chr4.1:31080726-31080745 MS.gene003631:CDS 35.0%
CAATCTAACTGAACTTAGCA+AGG - chr4.1:31080158-31080177 None:intergenic 35.0%
CAATTCAATCAGGCCAATAA+GGG - chr4.1:31079262-31079281 None:intergenic 35.0%
CATGTACAATCGACATTTCT+AGG - chr4.1:31081030-31081049 None:intergenic 35.0%
CCGAATCTGAATCAAAAGAA+AGG - chr4.1:31078697-31078716 None:intergenic 35.0%
CTAGTTTCAATAATCCCACA+AGG + chr4.1:31079816-31079835 MS.gene003631:CDS 35.0%
CTATCATCAAAAGGGAAATG+TGG + chr4.1:31082597-31082616 MS.gene003631:intron 35.0%
GATCACAGTATCACACAAAA+GGG - chr4.1:31080000-31080019 None:intergenic 35.0%
GTCCATCTATCAAACTTATC+TGG - chr4.1:31082996-31083015 None:intergenic 35.0%
TAAAAAAGCTTCAGAGGGAT+CGG - chr4.1:31080543-31080562 None:intergenic 35.0%
TCAATTCAATCAGGCCAATA+AGG - chr4.1:31079263-31079282 None:intergenic 35.0%
TTGCAAAGTAACATACTAGG+AGG - chr4.1:31080629-31080648 None:intergenic 35.0%
! ATGATGCTTACGTCCAATTT+TGG + chr4.1:31081898-31081917 MS.gene003631:intron 35.0%
! CAAGGTATCAATCACATAAG+TGG + chr4.1:31079720-31079739 MS.gene003631:intron 35.0%
! GATTCTTGTTTGTGACCAAA+TGG + chr4.1:31081308-31081327 MS.gene003631:intron 35.0%
! TGATGCTTACGTCCAATTTT+GGG + chr4.1:31081899-31081918 MS.gene003631:intron 35.0%
! TTACATCTTTTCCAATGCGT+AGG + chr4.1:31082942-31082961 MS.gene003631:intron 35.0%
!! AAGTGGACTATATATGTTCG+TGG + chr4.1:31079737-31079756 MS.gene003631:CDS 35.0%
!!! AGGTAAACACTTTTGGGTTT+GGG + chr4.1:31079309-31079328 MS.gene003631:intron 35.0%
AAAAAAGCTTCAGAGGGATC+GGG - chr4.1:31080542-31080561 None:intergenic 40.0%
AACAGGCTTCTTTGTGGAAA+AGG - chr4.1:31080489-31080508 None:intergenic 40.0%
ACGAGCTAAAAAAGCTTCAG+AGG - chr4.1:31080549-31080568 None:intergenic 40.0%
AGGTTGTATCCAGATGATGA+AGG + chr4.1:31080458-31080477 MS.gene003631:CDS 40.0%
ATGCACTCCAAAAAAGGGTA+TGG - chr4.1:31078749-31078768 None:intergenic 40.0%
CAAAGTAACATACTAGGAGG+AGG - chr4.1:31080626-31080645 None:intergenic 40.0%
CAAGAATCCTTTACATGCAC+AGG - chr4.1:31081296-31081315 None:intergenic 40.0%
CAATAATCCCACAAGGATTG+TGG + chr4.1:31079823-31079842 MS.gene003631:CDS 40.0%
CATGTATCTACTTCCGCAAA+TGG - chr4.1:31082811-31082830 None:intergenic 40.0%
CGAGCTAAAAAAGCTTCAGA+GGG - chr4.1:31080548-31080567 None:intergenic 40.0%
CGCCAGATAAGTTTGATAGA+TGG + chr4.1:31082991-31083010 MS.gene003631:CDS 40.0%
CGTTTACTAGCATCTTCGAT+TGG - chr4.1:31080728-31080747 None:intergenic 40.0%
CTGAGAAATTCAGGAACCAT+TGG - chr4.1:31080784-31080803 None:intergenic 40.0%
GTAGTTACCTGTGCATGTAA+AGG + chr4.1:31081286-31081305 MS.gene003631:intron 40.0%
GTATGCTAAACTCTGTGTGA+TGG + chr4.1:31082455-31082474 MS.gene003631:intron 40.0%
TCTGTGATCATGCTCTATAG+AGG + chr4.1:31082399-31082418 MS.gene003631:intron 40.0%
TGTAAAGTCTTGCAGTACTG+TGG + chr4.1:31080125-31080144 MS.gene003631:intron 40.0%
TGTATCTACTTCCGCAAATG+GGG - chr4.1:31082809-31082828 None:intergenic 40.0%
! ATCCTTTAACGAAGAAGCAC+AGG - chr4.1:31082206-31082225 None:intergenic 40.0%
! CATCTGCTTCTGAGAAATTC+AGG - chr4.1:31080793-31080812 None:intergenic 40.0%
! TATACGGGAACGTAGCATTT+TGG + chr4.1:31079559-31079578 MS.gene003631:CDS 40.0%
! TTTAGGATCCCTCCATTTCA+TGG + chr4.1:31081613-31081632 MS.gene003631:intron 40.0%
!! ATTCAGGAACCATTGGCTTA+AGG - chr4.1:31080777-31080796 None:intergenic 40.0%
!! TTCAGGAACCATTGGCTTAA+GGG - chr4.1:31080776-31080795 None:intergenic 40.0%
!!! ACCTCCTTTTGAGTTATCAG+AGG + chr4.1:31079850-31079869 MS.gene003631:CDS 40.0%
!!! GAGGTAAACACTTTTGGGTT+TGG + chr4.1:31079308-31079327 MS.gene003631:intron 40.0%
AAAAAGCTTCAGAGGGATCG+GGG - chr4.1:31080541-31080560 None:intergenic 45.0%
AAGGAACTGCCTTCATCATC+TGG - chr4.1:31080470-31080489 None:intergenic 45.0%
AGTCTTGGCATGTTCACAAC+TGG - chr4.1:31080584-31080603 None:intergenic 45.0%
ATGAGCTTGAACCTACGCAT+TGG - chr4.1:31082956-31082975 None:intergenic 45.0%
CAAACGACTCACAAGTTCAC+AGG - chr4.1:31079938-31079957 None:intergenic 45.0%
CAATTTGCCATAGTCACCTG+CGG + chr4.1:31080646-31080665 MS.gene003631:intron 45.0%
CACAAAGAAGCCTGTTGTTG+TGG + chr4.1:31080493-31080512 MS.gene003631:CDS 45.0%
CACAACAACAGGCTTCTTTG+TGG - chr4.1:31080495-31080514 None:intergenic 45.0%
CACCTGTGCTTCTTCGTTAA+AGG + chr4.1:31082201-31082220 MS.gene003631:intron 45.0%
CCTGCCTCTGATAACTCAAA+AGG - chr4.1:31079857-31079876 None:intergenic 45.0%
GATCTCTTGCTTCAGTGTAG+AGG + chr4.1:31080016-31080035 MS.gene003631:intron 45.0%
GCCTCTGATAACTCAAAAGG+AGG - chr4.1:31079854-31079873 None:intergenic 45.0%
GCTACGTTCCCGTATACTAT+TGG - chr4.1:31079555-31079574 None:intergenic 45.0%
GTCTTGGCATGTTCACAACT+GGG - chr4.1:31080583-31080602 None:intergenic 45.0%
TAACATACTAGGAGGAGGCA+AGG - chr4.1:31080621-31080640 None:intergenic 45.0%
TCGAAGATGCTAGTAAACGG+AGG + chr4.1:31080729-31080748 MS.gene003631:CDS 45.0%
TGAGTTATCAGAGGCAGGAT+GGG + chr4.1:31079859-31079878 MS.gene003631:CDS 45.0%
TGCTTATGTGTCCCCATTTG+CGG + chr4.1:31082795-31082814 MS.gene003631:intron 45.0%
TGTGGAAAAATGAAAGCCGC+AGG - chr4.1:31080665-31080684 None:intergenic 45.0%
TTGAGTTATCAGAGGCAGGA+TGG + chr4.1:31079858-31079877 MS.gene003631:CDS 45.0%
! CATGTTGATTGCTCGAGTTG+TGG + chr4.1:31082512-31082531 MS.gene003631:intron 45.0%
! GGCAACACAGTTTTCTCACA+AGG - chr4.1:31082910-31082929 None:intergenic 45.0%
! TGTACTGACATGTGCATGAG+AGG + chr4.1:31081989-31082008 MS.gene003631:intron 45.0%
!!! CCTTTTGAGTTATCAGAGGC+AGG + chr4.1:31079854-31079873 MS.gene003631:CDS 45.0%
!!! TTTAAAATATATAATATTTG+TGG + chr4.1:31080867-31080886 MS.gene003631:intron 5.0%
AACATGCCAAGACTACCTGC+TGG + chr4.1:31080590-31080609 MS.gene003631:CDS 50.0%
ACATGCCAAGACTACCTGCT+GGG + chr4.1:31080591-31080610 MS.gene003631:CDS 50.0%
AGCAGATGAACTCTTGCAGC+TGG + chr4.1:31080805-31080824 MS.gene003631:CDS 50.0%
AGGTGCATCCACAATCCTTG+TGG - chr4.1:31079834-31079853 None:intergenic 50.0%
ATGAAAGCCGCAGGTGACTA+TGG - chr4.1:31080656-31080675 None:intergenic 50.0%
CACGCTTTACAATAACCCCC+AGG - chr4.1:31079778-31079797 None:intergenic 50.0%
CCACCTCACCATGAAATGGA+GGG - chr4.1:31081624-31081643 None:intergenic 50.0%
GAGTTATCAGAGGCAGGATG+GGG + chr4.1:31079860-31079879 MS.gene003631:CDS 50.0%
GATCCCTCCATTTCATGGTG+AGG + chr4.1:31081618-31081637 MS.gene003631:intron 50.0%
GATGCTAGTAAACGGAGGAG+AGG + chr4.1:31080734-31080753 MS.gene003631:CDS 50.0%
GCTTGTCGCAAACATCACTG+TGG - chr4.1:31079916-31079935 None:intergenic 50.0%
GGTGCATCCACAATCCTTGT+GGG - chr4.1:31079833-31079852 None:intergenic 50.0%
TCAGCCACCTCACCATGAAA+TGG - chr4.1:31081628-31081647 None:intergenic 50.0%
! GCTCGAGTTGTGGTCCATTT+GGG + chr4.1:31082522-31082541 MS.gene003631:intron 50.0%
! GGTCTTGTGTCGACCCTTAT+TGG + chr4.1:31079246-31079265 MS.gene003631:intron 50.0%
! TGCTCGAGTTGTGGTCCATT+TGG + chr4.1:31082521-31082540 MS.gene003631:intron 50.0%
!!! GGGAACGTAGCATTTTGGCT+TGG + chr4.1:31079564-31079583 MS.gene003631:CDS 50.0%
AATAACCCCCAGGTCCTCGT+TGG - chr4.1:31079768-31079787 None:intergenic 55.0%
CCCTCCATTTCATGGTGAGG+TGG + chr4.1:31081621-31081640 MS.gene003631:intron 55.0%
GCCACCTCACCATGAAATGG+AGG - chr4.1:31081625-31081644 None:intergenic 55.0%
GTAAGCCCAGCAGGTAGTCT+TGG - chr4.1:31080599-31080618 None:intergenic 55.0%
! TGTTCGTGGAGCATCCAACG+AGG + chr4.1:31079751-31079770 MS.gene003631:CDS 55.0%
AGCATCCAACGAGGACCTGG+GGG + chr4.1:31079760-31079779 MS.gene003631:CDS 60.0%
GAGCATCCAACGAGGACCTG+GGG + chr4.1:31079759-31079778 MS.gene003631:CDS 60.0%
! GGAGCATCCAACGAGGACCT+GGG + chr4.1:31079758-31079777 MS.gene003631:CDS 60.0%
! TGGAGCATCCAACGAGGACC+TGG + chr4.1:31079757-31079776 MS.gene003631:CDS 60.0%
GGAGGCAAGGTAAGCCCAGC+AGG - chr4.1:31080608-31080627 None:intergenic 65.0%
GCTGGCAGCAGCTCGTCAGC+AGG + chr4.1:31080823-31080842 MS.gene003631:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31078660 31083050 31078660 ID=MS.gene003631
chr4.1 mRNA 31078660 31083050 31078660 ID=MS.gene003631.t1;Parent=MS.gene003631
chr4.1 exon 31078660 31078689 31078660 ID=MS.gene003631.t1.exon1;Parent=MS.gene003631.t1
chr4.1 CDS 31078660 31078689 31078660 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
chr4.1 exon 31079513 31079601 31079513 ID=MS.gene003631.t1.exon2;Parent=MS.gene003631.t1
chr4.1 CDS 31079513 31079601 31079513 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
chr4.1 exon 31079724 31079945 31079724 ID=MS.gene003631.t1.exon3;Parent=MS.gene003631.t1
chr4.1 CDS 31079724 31079945 31079724 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
chr4.1 exon 31080445 31080632 31080445 ID=MS.gene003631.t1.exon4;Parent=MS.gene003631.t1
chr4.1 CDS 31080445 31080632 31080445 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
chr4.1 exon 31080723 31080844 31080723 ID=MS.gene003631.t1.exon5;Parent=MS.gene003631.t1
chr4.1 CDS 31080723 31080844 31080723 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
chr4.1 exon 31082964 31083050 31082964 ID=MS.gene003631.t1.exon6;Parent=MS.gene003631.t1
chr4.1 CDS 31082964 31083050 31082964 ID=cds.MS.gene003631.t1;Parent=MS.gene003631.t1
Gene Sequence

>MS.gene003631

ATGGTCAAATCCGAAGACAATGATAAGAAGGTAACCTTTCTTTTGATTCAGATTCGGTTAAAATCCTAAAATAACGGTTTTTAAAACCATACCCTTTTTTGGAGTGCATAAAGTTTGCACCTTTATTCAAATTTGAAGCTTTGATTTGGTACAAACTTATCACACTGCAATAACCTTGAAATCAATCTGTAGAATGTTGAATTTGAAAATGATTTTTGAATGTAAATGCAATGTGAAGAGAAATTTGTCTATTTTAGTGAAATAGAGTCTTTTATTGATAATGAGCTTGGTTGAATTGAATTATTTAAGCTTATTTACTGATACAAACATTTGTGATACTATCTGGGAAGAGCTTATAGAAATAGCTTATAACATTTCCTTACGATGTTTTTAGCTTATTTCAATAAGCTTTACTGAATAGTTTATGAAAACAACTTATATGCAAATAGTTTGCCTTTATTTTTTCTTTTACTATAGAAATAGATTATGAATATGCACTTACATTACTATAAGTGTTAAATTAATTTGTTTATTCAAATATGTCATATATTATTTGAGCTTGATTGAATTGTTCAAACAATTTTTTGGTCTTGTGTCGACCCTTATTGGCCTGATTGAATTGAATTGAATTATTTGAGCTTATCTATTGAGGTAAACACTTTTGGGTTTGGGAGAACTAGTCGAAACAACTTATAACATTTTCTTAAGCTATTTTTAGCTTTTTATATAAATTTTCCATGATAGCTTAATGAAAACAGCTTATAGTTTGTAGCTTATATGCAAACAATTTGTCTTTATTTTATCTTTTGTTACATAAAAAGCATAATTAAGTGCTTATATTATTTTTTTGTAGAACTCGGGTAAGAAACTCAAAGATGTAGAAATTAGTGTTCCAATAGTATACGGGAACGTAGCATTTTGGCTTGGCAAAAAAGCTAGTGAGTAAGTAACTTACATTGCATCCTTCAATTGTTGTTTTAATTAATTAATAAATGTATTGCTTAAATGGGGTTTCCTTCACACAAAAAAAATGTGCTAAGGTGACAATGTTTATGTTATACAAGGTATCAATCACATAAGTGGACTATATATGTTCGTGGAGCATCCAACGAGGACCTGGGGGTTATTGTAAAGCGTGTTGTTTTTCAGTTGCATACTAGTTTCAATAATCCCACAAGGATTGTGGATGCACCTCCTTTTGAGTTATCAGAGGCAGGATGGGGAGAATTTGAAATTGCCATCACACTTTATTTCCACAGTGATGTTTGCGACAAGCCTGTGAACTTGTGAGTCGTTTGATGTTGTTTTTGATTTATGGGGAAAATAAAGTTTCTTTACCCTTTTGTGTGATACTGTGATCTCTTGCTTCAGTGTAGAGGTTCAAAGTATGCTCAAAAGTAGGGAAAATCTCAAATAGTGTTTTAAGTCCTGTTTGTTACATATCATTAACAATGTGCTTTTGTTTTGTAAAGTCTTGCAGTACTGTGGCTCTATGCCTTGCTAAGTTCAGTTAGATTGAGTTAGTAGTTTGGTTTGCTTTTGTGCCGCATTTTATTAATAATGATAAGCTTAAATGATTATAAAACAACTAATATCTATCTTGTTATGTTTTTATTAGAGCATGCAGCTATACGATTCCAATGCTTGAAAATTATTCATTGATATACTACATGACCATAGGTCTATTATTCACTGTTATAAATCATGCAGATTGCAAACCATATTTTGTGAATGTCAAAATAATTTCTGAATCTTGTTTTTTATTGAAGTGTAATGTATATACAGATATCATCAGTTGAGGTTGTATCCAGATGATGAAGGCAGTTCCTTTTCCACAAAGAAGCCTGTTGTTGTGGAATTTTATGATGAGGTAGTTTTCCCCGATCCCTCTGAAGCTTTTTTAGCTCGTGTGCAGAATCACCCAGTTGTGAACATGCCAAGACTACCTGCTGGGCTTACCTTGCCTCCTCCTAGTATGTTACTTTGCAATTTGCCATAGTCACCTGCGGCTTTCATTTTTCCACATTTGAGTTTAATTAACGTTACATTATCTATAAATGCAGTTCCAATCGAAGATGCTAGTAAACGGAGGAGAGGTGATACTAAAGATAATCCCTTAAGCCAATGGTTCCTGAATTTCTCAGAAGCAGATGAACTCTTGCAGCTGGCAGCAGCTCGTCAGCAGGTATGATTGACATAATATCATATTTAAAATATATAATATTTGTGGAAGTCGAAGTAGTTGTTGTTGCCATGATGATATATTTATTTGAATTTGAATCAACAATTTACAGCAAGTATATGGTTGCATCCTTACTAATAATGAAGTCTGTATCATACATGTACACATGTTTGGCTGTTTATTACCCTAGAAATGTCGATTGTACATGAGTGTGATAGCATCAAGTTTTATGATTTTGTTCTAAATTTCTCTCCACACTTCTAAACATATAATCAAGAATCTTCGGTTAAAGATTCAAAACTTCTGTTCTATACTTGAAGTTTATTTTTGTTTACGTGATGCTTATGGCCCACTTTTGTGATTATTCATTGACTCAAAATGATTATGCCTTTGTATTAATTAGAATAGATTACATCTCATTTTTCTTAAATAAGATTATTGGTTGTAGTTACCTGTGCATGTAAAGGATTCTTGTTTGTGACCAAATGGTTTTATTCAAAATATGTCATAGTTATCTTGTACTATCTCTTGTGAGTGTTCATCATTTAAGGATTTGTTTCTGGCATCCATTATCATCAATATATTGTGGATTAGGTTGTTCTAATAATTTCTATGTGGGGCATGAATGTTCAGAGTGTATATCATAATGCTATTTTATACTAATAAATATGCTTAAATAAATTTCTGGTCTTGGTAATAGAAGCTTTGTTCCTTACAAAAAAATGTGTACTTCTATATGAAGAAAGAAAAATCTATTTAATCTTTTAGTTATTTAGGATCCCTCCATTTCATGGTGAGGTGGCTGACAGTGACATGATTCCACCTTACTTTTACATATGGTACTTATTTGTTCTTTATTTAAAATTTATATAATATTTGTATAAACAAACAAAAATAGAAATTAAAAAGTTAGTTTAACTAGGGTTAAAAGTTAGAATTTGCACTGTATATTTAATGGAGAACCTCTTTCCTAAATATTTTCTTTGTTCTTCGTTCATAACATATAATGATGACTTATCAGTTTTTTTTCTATGCTTCAACTCTATTTTTGTTTGGATGATGCTTACGTCCAATTTTGGGATTAGTAGAGTTAAAATAATTGTCTTCGAATTATTAGTACAAATTTTACTCAAATAAGATTGTTGGTTGTACTGACATGTGCATGAGAGGAATTCCTGTTTGTAACTAAATGATTTAATTCAACTTCTAAAGAAAGAGCTTGTTCTTCATTGAAGAGTTTGCTTCTGTCATCCTCTATTATCTTTCATCAATTTACAGGTCAGAGAGAAAAGACAGAAAAGATATTGTACATGCAAATTAACATTATTTCAATTTTATGTAATAGCTATCTCGTACTATCACCTGTGCTTCTTCGTTAAAGGATTTGCTTCTGACATCCATTATTATCAATTGTGGGTCATGTGTGTTCAAATAATTTCTATGCCTAGCTTAATATTCAAAATGCTTATCATAATGCACTTCTAAATAATAAATACTAGTAAGTCATTCTATGTCATAAGACCAAAGATATACTGTTTCCATGATTTACTTTTTATTTCTGTGATCATGCTCTATAGAGGAATTTATTTTCTCCAAATCATTGACCATGTTCAGTATGCTAAACTCTGTGTGATGGTCCTTTTCCTTGTATTTTATTATTAAGTTGTTGTCATGTTGATTGCTCGAGTTGTGGTCCATTTGGGTAAACAAAGTAGTTAAGTGCTTATCATATAAGCGTTTATGAATAAGTTAATTCTATCATCAAAAGGGAAATGTGGTTAAACTACTTAGATACAAGGTGTGTGTTTTCAAAAATCACAGGCTATTGTGTATAATTTACTGAAACAAGTCGACGAAAATAGTGTATGGACAAGTCATAAACTGTTTCATGAGTTAACTCAAACTACATGAGTGCTTATATTATAAGATTATCTTAAATAAGCTAATTATTGATGCTTATGTGTCCCCATTTGCGGAAGTAGATACATGCTGAATAATACTTGTTGCACAAATTATAATATGCTTTACTCTTTGTTGAAGACTTTGAATGACTCTATCATCTTAACCTTGTGAGAAAACTGTGTTGCCAACTGATACTTATTACATCTTTTCCAATGCGTAGGTTCAAGCTCATATTTCGAAACTCAAACGCCAGATAAGTTTGATAGATGGACAGCATAAGCAGCTTAAATCTTCTCCTGACCAATAG
Protein sequence

>MS.gene003631.t1

MVKSEDNDKKNSGKKLKDVEISVPIVYGNVAFWLGKKASEYQSHKWTIYVRGASNEDLGVIVKRVVFQLHTSFNNPTRIVDAPPFELSEAGWGEFEIAITLYFHSDVCDKPVNLYHQLRLYPDDEGSSFSTKKPVVVEFYDEVVFPDPSEAFLARVQNHPVVNMPRLPAGLTLPPPIPIEDASKRRRGDTKDNPLSQWFLNFSEADELLQLAAARQQVQAHISKLKRQISLIDGQHKQLKSSPDQ