AlfalfaGEDB | MS.gene60120

M. sativa cultivar XinJiangDaYe / MS.gene60120

KEGG: K09548
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF01920.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60120.t1	AET02323.2	99	104	1	0	1	104	18	121	1.60E-45	191.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60120.t1	Q3SZE2	38.1	84	52	0	6	89	33	116	7.7e-09	61.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60120.t1	G7LG18	99.0	104	1	0	1	104	18	121	1.1e-45	191.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene60120	0.822936	1.81E-53	-1.69E-46
MS.gene049786	MS.gene60120	0.810604	1.06E-50	-1.69E-46
MS.gene050114	MS.gene60120	0.841808	3.80E-58	-1.69E-46
MS.gene050317	MS.gene60120	0.813841	2.09E-51	-1.69E-46
MS.gene050320	MS.gene60120	0.827315	1.67E-54	-1.69E-46
MS.gene050439	MS.gene60120	0.805715	1.17E-49	-1.69E-46
MS.gene051003	MS.gene60120	0.811822	5.78E-51	-1.69E-46
MS.gene051063	MS.gene60120	0.813896	2.03E-51	-1.69E-46
MS.gene051245	MS.gene60120	0.817266	3.60E-52	-1.69E-46
MS.gene051452	MS.gene60120	0.805728	1.16E-49	-1.69E-46
MS.gene051655	MS.gene60120	0.809271	2.06E-50	-1.69E-46
MS.gene051746	MS.gene60120	0.803559	3.29E-49	-1.69E-46
MS.gene051748	MS.gene60120	0.819769	9.76E-53	-1.69E-46
MS.gene051756	MS.gene60120	0.804118	2.52E-49	-1.69E-46
MS.gene051757	MS.gene60120	0.820282	7.45E-53	-1.69E-46
MS.gene05188	MS.gene60120	0.827422	1.58E-54	-1.69E-46
MS.gene051903	MS.gene60120	0.821854	3.24E-53	-1.69E-46
MS.gene051975	MS.gene60120	0.831478	1.63E-55	-1.69E-46
MS.gene052434	MS.gene60120	0.819792	9.64E-53	-1.69E-46
MS.gene053109	MS.gene60120	0.833957	3.96E-56	-1.69E-46
MS.gene053298	MS.gene60120	0.806673	7.34E-50	-1.69E-46
MS.gene053340	MS.gene60120	0.806438	8.23E-50	-1.69E-46
MS.gene053381	MS.gene60120	0.805288	1.44E-49	-1.69E-46
MS.gene053952	MS.gene60120	0.801204	1.01E-48	-1.69E-46
MS.gene05469	MS.gene60120	0.809652	1.70E-50	-1.69E-46
MS.gene055603	MS.gene60120	0.854091	1.55E-61	-1.69E-46
MS.gene056403	MS.gene60120	0.828201	1.03E-54	-1.69E-46
MS.gene056605	MS.gene60120	0.828516	8.61E-55	-1.69E-46
MS.gene056631	MS.gene60120	0.824333	8.54E-54	-1.69E-46
MS.gene056638	MS.gene60120	0.845596	3.68E-59	-1.69E-46
MS.gene056752	MS.gene60120	0.813904	2.02E-51	-1.69E-46
MS.gene056787	MS.gene60120	0.800987	1.11E-48	-1.69E-46
MS.gene056986	MS.gene60120	0.862424	5.12E-64	-1.69E-46
MS.gene057094	MS.gene60120	0.82472	6.93E-54	-1.69E-46
MS.gene057140	MS.gene60120	0.806191	9.27E-50	-1.69E-46
MS.gene057234	MS.gene60120	0.800667	1.29E-48	-1.69E-46
MS.gene05781	MS.gene60120	0.803429	3.50E-49	-1.69E-46
MS.gene057999	MS.gene60120	0.832944	7.08E-56	-1.69E-46
MS.gene058238	MS.gene60120	0.841071	5.93E-58	-1.69E-46
MS.gene058654	MS.gene60120	0.80056	1.36E-48	-1.69E-46
MS.gene058906	MS.gene60120	0.812196	4.79E-51	-1.69E-46
MS.gene058911	MS.gene60120	0.803311	3.70E-49	-1.69E-46
MS.gene059360	MS.gene60120	0.818279	2.13E-52	-1.69E-46
MS.gene059830	MS.gene60120	0.811163	8.03E-51	-1.69E-46
MS.gene06001	MS.gene60120	0.824966	6.06E-54	-1.69E-46
MS.gene06010	MS.gene60120	0.819913	9.05E-53	-1.69E-46
MS.gene06011	MS.gene60120	0.866806	2.18E-65	-1.69E-46
MS.gene060586	MS.gene60120	0.847878	8.75E-60	-1.69E-46
MS.gene060830	MS.gene60120	0.813445	2.55E-51	-1.69E-46
MS.gene060869	MS.gene60120	0.843284	1.54E-58	-1.69E-46
MS.gene060870	MS.gene60120	0.81265	3.81E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene60120	MS.gene50691	PPI
MS.gene60120	MS.gene23366	PPI
MS.gene60120	MS.gene062939	PPI
MS.gene60120	MS.gene003631	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60120.t1	MTR_8g039140	99.038	104	1	0	1	104	18	121	5.03e-71	207
MS.gene60120.t1	MTR_8g039140	99.029	103	1	0	2	104	10	112	2.46e-70	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60120.t1	AT2G07340	68.932	103	32	0	2	104	26	128	2.00e-49	153
MS.gene60120.t1	AT2G07340	68.932	103	31	1	2	104	26	127	3.54e-47	147

Find 16 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTTTCTTTGGAACGTATC+TGG	0.128232	8.4:-59271969	None:intergenic
ACGCTTCTTGTCTGTTTCTT+TGG	0.277466	8.4:-59271980	None:intergenic
AATTGTGGCCAGGTTTGTAT+TGG	0.395225	8.4:+59272300	MS.gene60120:intron
TGTTTCTTTGGAACGTATCT+GGG	0.410800	8.4:-59271968	None:intergenic
GACAATGGAGGAACTGAAGC+AGG	0.447626	8.4:+59272011	MS.gene60120:CDS
TTGTAGACATTAGTATCGTC+AGG	0.474681	8.4:-59272036	None:intergenic
ACAGAGCTCAAAAGAATACT+TGG	0.492644	8.4:+59272599	MS.gene60120:intron
GGCAACGTTAATGAACGAAC+AGG	0.526861	8.4:+59272330	MS.gene60120:CDS
CGAACAGGAGAACAAGTTCA+AGG	0.550157	8.4:+59272345	MS.gene60120:CDS
ACTAATGTCTACAAATCCAT+TGG	0.553731	8.4:+59272045	MS.gene60120:CDS
GAGCTGTTACAACAAGATCC+TGG	0.568394	8.4:+59272657	MS.gene60120:CDS
GGAGAAGCAGATTGCAGAGG+TGG	0.574472	8.4:+59272620	MS.gene60120:CDS
CAGGTTTGTATTGGAGACGA+AGG	0.626778	8.4:+59272309	MS.gene60120:intron
AATGACATTTACTTGTAATG+CGG	0.628391	8.4:-59272386	None:intergenic
ATAATTTGACGAGCAAGACC+AGG	0.645415	8.4:-59272675	None:intergenic
CTTGGAGAAGCAGATTGCAG+AGG	0.694862	8.4:+59272617	MS.gene60120:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTAAATTTTTAAAGTTGTA+AGG	+	chr8.4:59272462-59272481	MS.gene60120:intron	10.0%
!!!	TATAATTAATCAGTCTATTT+TGG	+	chr8.4:59272157-59272176	MS.gene60120:intron	15.0%
!!!	TATATTTCTGAAGAAAAATA+GGG	-	chr8.4:59272141-59272160	None:intergenic	15.0%
!!!	TTATATTTCTGAAGAAAAAT+AGG	-	chr8.4:59272142-59272161	None:intergenic	15.0%
!!	ATACAATTCTAAATACTTGT+AGG	-	chr8.4:59272097-59272116	None:intergenic	20.0%
!!!	ATTTTGTAGCATGTTTAAAT+AGG	-	chr8.4:59272213-59272232	None:intergenic	20.0%
!	AATGACATTTACTTGTAATG+CGG	-	chr8.4:59272389-59272408	None:intergenic	25.0%
!	AGAAGTTTCAATAAAAGTGA+AGG	-	chr8.4:59272412-59272431	None:intergenic	25.0%
!	TGGTATGTAAAAGATATCTT+AGG	+	chr8.4:59272065-59272084	MS.gene60120:intron	25.0%
!!	AGATATCTTTTACATACCAA+TGG	-	chr8.4:59272064-59272083	None:intergenic	25.0%
	ACTAATGTCTACAAATCCAT+TGG	+	chr8.4:59272045-59272064	MS.gene60120:CDS	30.0%
	CTAATACACCTCTTAAATTG+TGG	+	chr8.4:59272285-59272304	MS.gene60120:intron	30.0%
	GTCTGAGTGAGAAAATAATA+TGG	+	chr8.4:59272570-59272589	MS.gene60120:intron	30.0%
	GTGAGAAAATAATATGGCTT+TGG	+	chr8.4:59272576-59272595	MS.gene60120:intron	30.0%
!!!	AGTCTATTTTGGTTTTTGCA+GGG	+	chr8.4:59272168-59272187	MS.gene60120:intron	30.0%
	ACAGAGCTCAAAAGAATACT+TGG	+	chr8.4:59272599-59272618	MS.gene60120:intron	35.0%
	AGAACAAAAGCTTCGTTGTT+GGG	+	chr8.4:59272235-59272254	MS.gene60120:intron	35.0%
	TTGTAGACATTAGTATCGTC+AGG	-	chr8.4:59272039-59272058	None:intergenic	35.0%
!!	TGTTTCTTTGGAACGTATCT+GGG	-	chr8.4:59271971-59271990	None:intergenic	35.0%
!!!	CAGTCTATTTTGGTTTTTGC+AGG	+	chr8.4:59272167-59272186	MS.gene60120:intron	35.0%
	AAACCTGGCCACAATTTAAG+AGG	-	chr8.4:59272296-59272315	None:intergenic	40.0%
	AATTGTGGCCAGGTTTGTAT+TGG	+	chr8.4:59272300-59272319	MS.gene60120:intron	40.0%
	ATAATTTGACGAGCAAGACC+AGG	-	chr8.4:59272678-59272697	None:intergenic	40.0%
	GAGAACAAAAGCTTCGTTGT+TGG	+	chr8.4:59272234-59272253	MS.gene60120:intron	40.0%
!	ACGCTTCTTGTCTGTTTCTT+TGG	-	chr8.4:59271983-59272002	None:intergenic	40.0%
!!	CTGTTTCTTTGGAACGTATC+TGG	-	chr8.4:59271972-59271991	None:intergenic	40.0%
!!!	GAAGCGTGCTTTTTTGACAA+TGG	+	chr8.4:59271996-59272015	MS.gene60120:CDS	40.0%
	ACACCTCTTAAATTGTGGCC+AGG	+	chr8.4:59272290-59272309	MS.gene60120:intron	45.0%
	CAGGTTTGTATTGGAGACGA+AGG	+	chr8.4:59272309-59272328	MS.gene60120:intron	45.0%
	CGAACAGGAGAACAAGTTCA+AGG	+	chr8.4:59272345-59272364	MS.gene60120:CDS	45.0%
	CTTCGTCTCCAATACAAACC+TGG	-	chr8.4:59272311-59272330	None:intergenic	45.0%
	GAGCTGTTACAACAAGATCC+TGG	+	chr8.4:59272657-59272676	MS.gene60120:CDS	45.0%
	GGCAACGTTAATGAACGAAC+AGG	+	chr8.4:59272330-59272349	MS.gene60120:CDS	45.0%
!!!	GCGTGCTTTTTTGACAATGG+AGG	+	chr8.4:59271999-59272018	MS.gene60120:CDS	45.0%
	CTTGGAGAAGCAGATTGCAG+AGG	+	chr8.4:59272617-59272636	MS.gene60120:CDS	50.0%
	GACAATGGAGGAACTGAAGC+AGG	+	chr8.4:59272011-59272030	MS.gene60120:CDS	50.0%
	TGCAGGGAAAACGTAAGTCG+TGG	+	chr8.4:59272184-59272203	MS.gene60120:intron	50.0%
	GGAGAAGCAGATTGCAGAGG+TGG	+	chr8.4:59272620-59272639	MS.gene60120:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	59271955	59272713	59271955	ID=MS.gene60120
chr8.4	mRNA	59271955	59272713	59271955	ID=MS.gene60120.t1;Parent=MS.gene60120
chr8.4	exon	59271955	59272066	59271955	ID=MS.gene60120.t1.exon1;Parent=MS.gene60120.t1
chr8.4	CDS	59271955	59272066	59271955	ID=cds.MS.gene60120.t1;Parent=MS.gene60120.t1
chr8.4	exon	59272189	59272195	59272189	ID=MS.gene60120.t1.exon2;Parent=MS.gene60120.t1
chr8.4	CDS	59272189	59272195	59272189	ID=cds.MS.gene60120.t1;Parent=MS.gene60120.t1
chr8.4	exon	59272312	59272396	59272312	ID=MS.gene60120.t1.exon3;Parent=MS.gene60120.t1
chr8.4	CDS	59272312	59272396	59272312	ID=cds.MS.gene60120.t1;Parent=MS.gene60120.t1
chr8.4	exon	59272603	59272713	59272603	ID=MS.gene60120.t1.exon4;Parent=MS.gene60120.t1
chr8.4	CDS	59272603	59272713	59272603	ID=cds.MS.gene60120.t1;Parent=MS.gene60120.t1

Gene Sequence

>MS.gene60120

ATGCAGGTGCAAACCCAGATACGTTCCAAAGAAACAGACAAGAAGCGTGCTTTTTTGACAATGGAGGAACTGAAGCAGGTTCCTGACGATACTAATGTCTACAAATCCATTGGTATGTAAAAGATATCTTAGGAATAATCCTACAAGTATTTAGAATTGTATTGCATATTTATTTATTAGATGCCCTATTTTTCTTCAGAAATATAATTAATCAGTCTATTTTGGTTTTTGCAGGGAAAACGTAAGTCGTGGTTGCCTATTTAAACATGCTACAAAATAGAGAACAAAAGCTTCGTTGTTGGGTTGATTAGAAAATATAAATGACATACTCTAATACACCTCTTAAATTGTGGCCAGGTTTGTATTGGAGACGAAGGCAACGTTAATGAACGAACAGGAGAACAAGTTCAAGGATAGTGAAACTTCAATTACCGCATTACAAGTAAATGTCATTCCTTCACTTTTATTGAAACTTCTATTCTTTCATCATTTTATGACTACATTGTTTTTAAATTTTTAAAGTTGTAAGGAAGAAAATTATAATTTCAAAAATGAGACAAAAATAGCAGTTTAACTTTGTAGTTAAATTTCTGAATAGTAAATGAGTTTCATGATGTCTGAGTGAGAAAATAATATGGCTTTGGACAGAGCTCAAAAGAATACTTGGAGAAGCAGATTGCAGAGGTGGAAAACAATTTGAGAGAGCTGTTACAACAAGATCCTGGTCTTGCTCGTCAAATTATGTCAATGAATGTGTAA

Protein sequence

>MS.gene60120.t1

MQVQTQIRSKETDKKRAFLTMEELKQVPDDTNVYKSIGKTFVLETKATLMNEQENKFKDSETSITALQSSKEYLEKQIAEVENNLRELLQQDPGLARQIMSMNV