AlfalfaGEDB | MS.gene88495

M. sativa cultivar XinJiangDaYe / MS.gene88495

KEGG: K09548
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0006457 GO:0008150 GO:0009987 GO:0016272 GO:0032991 GO:0051082
PFAM: PF01920.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88495.t1	AET02323.2	99	104	1	0	1	104	18	121	1.60E-45	191.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88495.t1	Q3SZE2	38.1	84	52	0	6	89	33	116	7.7e-09	61.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88495.t1	G7LG18	99.0	104	1	0	1	104	18	121	1.1e-45	191.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene88495	0.811614	6.41E-51	-1.69E-46
MS.gene049178	MS.gene88495	0.807477	4.96E-50	-1.69E-46
MS.gene049756	MS.gene88495	0.800509	1.39E-48	-1.69E-46
MS.gene04984	MS.gene88495	0.804009	2.65E-49	-1.69E-46
MS.gene050719	MS.gene88495	0.82436	8.42E-54	-1.69E-46
MS.gene050721	MS.gene88495	0.829127	6.12E-55	-1.69E-46
MS.gene050724	MS.gene88495	0.826575	2.52E-54	-1.69E-46
MS.gene050844	MS.gene88495	0.807924	3.99E-50	-1.69E-46
MS.gene051247	MS.gene88495	0.807984	3.87E-50	-1.69E-46
MS.gene051452	MS.gene88495	0.813275	2.78E-51	-1.69E-46
MS.gene051757	MS.gene88495	0.853186	2.82E-61	-1.69E-46
MS.gene05188	MS.gene88495	0.819227	1.30E-52	-1.69E-46
MS.gene051975	MS.gene88495	0.810678	1.02E-50	-1.69E-46
MS.gene052896	MS.gene88495	0.802406	5.70E-49	-1.69E-46
MS.gene055603	MS.gene88495	0.829324	5.49E-55	-1.69E-46
MS.gene055623	MS.gene88495	0.801466	8.89E-49	-1.69E-46
MS.gene056373	MS.gene88495	0.802348	5.85E-49	-1.69E-46
MS.gene056631	MS.gene88495	0.812912	3.34E-51	-1.69E-46
MS.gene056986	MS.gene88495	0.829407	5.24E-55	-1.69E-46
MS.gene058906	MS.gene88495	0.833799	4.33E-56	-1.69E-46
MS.gene058908	MS.gene88495	0.800113	1.68E-48	-1.69E-46
MS.gene058911	MS.gene88495	0.808429	3.11E-50	-1.69E-46
MS.gene059249	MS.gene88495	0.812516	4.08E-51	-1.69E-46
MS.gene06001	MS.gene88495	0.802117	6.53E-49	-1.69E-46
MS.gene06011	MS.gene88495	0.823244	1.54E-53	-1.69E-46
MS.gene060429	MS.gene88495	0.816434	5.54E-52	-1.69E-46
MS.gene060572	MS.gene88495	0.81674	4.73E-52	-1.69E-46
MS.gene060586	MS.gene88495	0.818299	2.11E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene88495	MS.gene062939	PPI
MS.gene88495	MS.gene50691	PPI
MS.gene88495	MS.gene003631	PPI
MS.gene88495	MS.gene23366	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88495.t1	MTR_8g039140	99.038	104	1	0	1	104	18	121	5.03e-71	207
MS.gene88495.t1	MTR_8g039140	99.029	103	1	0	2	104	10	112	2.46e-70	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88495.t1	AT2G07340	68.932	103	32	0	2	104	26	128	2.00e-49	153
MS.gene88495.t1	AT2G07340	68.932	103	31	1	2	104	26	127	3.54e-47	147

Find 17 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTTTCTTTGGAACGTATC+TGG	0.128232	8.3:+58252893	None:intergenic
ACGCTTCTTGTCTGTTTCTT+TGG	0.277466	8.3:+58252882	None:intergenic
AATTGTGGCCAGATTTGTAT+TGG	0.376369	8.3:-58252562	MS.gene88495:intron
TGTTTCTTTGGAACGTATCT+GGG	0.410800	8.3:+58252894	None:intergenic
CATAATTTGACGAGCAAGAC+CGG	0.429125	8.3:+58252181	None:intergenic
TTGTAGACATTAGTATCGTC+AGG	0.474681	8.3:+58252826	None:intergenic
GACAATGGAGGAGCTGAAGC+AGG	0.474978	8.3:-58252851	MS.gene88495:CDS
ACAGAGCTCAAAAGAATACT+TGG	0.492644	8.3:-58252258	MS.gene88495:intron
GGCAACGTTAATGAACGAAC+AGG	0.526861	8.3:-58252532	MS.gene88495:CDS
CGAACAGGAGAACAAGTTCA+AGG	0.550157	8.3:-58252517	MS.gene88495:CDS
ACTAATGTCTACAAATCCAT+TGG	0.553731	8.3:-58252817	MS.gene88495:intron
GGAGAAGCAGATTGCAGAGG+TGG	0.574472	8.3:-58252237	MS.gene88495:CDS
ATAATTTGACGAGCAAGACC+GGG	0.620149	8.3:+58252182	None:intergenic
CAGATTTGTATTGGAGACGA+AGG	0.620517	8.3:-58252553	MS.gene88495:CDS
GAGCTGTTACAACAAGATCC+CGG	0.623013	8.3:-58252200	MS.gene88495:CDS
AAGGACATTTACTTGTAATG+CGG	0.654969	8.3:+58252476	None:intergenic
CTTGGAGAAGCAGATTGCAG+AGG	0.694862	8.3:-58252240	MS.gene88495:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATAATTAATCAGTTTATTT+TGG	-	chr8.3:58252369-58252388	MS.gene88495:intron	10.0%
!!!	TATATTTCTGAAGAAAAATA+GGG	+	chr8.3:58252353-58252372	None:intergenic	15.0%
!!!	TTATATTTCTGAAGAAAAAT+AGG	+	chr8.3:58252354-58252373	None:intergenic	15.0%
!!	TCTAATACAATCTTAAATTG+TGG	-	chr8.3:58252496-58252515	MS.gene88495:CDS	20.0%
!!!	ATTTTGTAGCATGTTTAAAT+AGG	+	chr8.3:58252425-58252444	None:intergenic	20.0%
!	ATCTGAGTGAACAAATAATA+TGG	-	chr8.3:58252786-58252805	MS.gene88495:intron	25.0%
!	TGGTATGTAAAAGATATCTT+AGG	-	chr8.3:58252276-58252295	MS.gene88495:intron	25.0%
!!	AGATATCTTTTACATACCAA+TGG	+	chr8.3:58252275-58252294	None:intergenic	25.0%
!!!	AGTTTATTTTGGTTTTTGCA+GGG	-	chr8.3:58252380-58252399	MS.gene88495:intron	25.0%
	AAGGACATTTACTTGTAATG+CGG	+	chr8.3:58252600-58252619	None:intergenic	30.0%
	ACTAATGTCTACAAATCCAT+TGG	-	chr8.3:58252256-58252275	MS.gene88495:CDS	30.0%
	AGAACAAAAGCTTCATTGTT+GGG	-	chr8.3:58252447-58252466	MS.gene88495:intron	30.0%
	AGAAGTTTCCATAAAAGTGA+AGG	+	chr8.3:58252623-58252642	None:intergenic	30.0%
	GTGAACAAATAATATGGCTT+TGG	-	chr8.3:58252792-58252811	MS.gene88495:intron	30.0%
!!!	CAGTTTATTTTGGTTTTTGC+AGG	-	chr8.3:58252379-58252398	MS.gene88495:intron	30.0%
	AATTGTGGCCAGATTTGTAT+TGG	-	chr8.3:58252511-58252530	MS.gene88495:CDS	35.0%
	ACAGAGCTCAAAAGAATACT+TGG	-	chr8.3:58252815-58252834	MS.gene88495:intron	35.0%
	GAGAACAAAAGCTTCATTGT+TGG	-	chr8.3:58252446-58252465	MS.gene88495:intron	35.0%
	GTTTCCATAAAAGTGAAGGA+AGG	+	chr8.3:58252619-58252638	None:intergenic	35.0%
	TTGTAGACATTAGTATCGTC+AGG	+	chr8.3:58252250-58252269	None:intergenic	35.0%
!!	ATGTCCTTCCTTCACTTTTA+TGG	-	chr8.3:58252612-58252631	MS.gene88495:intron	35.0%
!!	TGTTTCTTTGGAACGTATCT+GGG	+	chr8.3:58252182-58252201	None:intergenic	35.0%
	ATAATTTGACGAGCAAGACC+GGG	+	chr8.3:58252894-58252913	None:intergenic	40.0%
	CAGATTTGTATTGGAGACGA+AGG	-	chr8.3:58252520-58252539	MS.gene88495:CDS	40.0%
	CATAATTTGACGAGCAAGAC+CGG	+	chr8.3:58252895-58252914	None:intergenic	40.0%
	CTTCGTCTCCAATACAAATC+TGG	+	chr8.3:58252522-58252541	None:intergenic	40.0%
!	ACGCTTCTTGTCTGTTTCTT+TGG	+	chr8.3:58252194-58252213	None:intergenic	40.0%
!!	CTGTTTCTTTGGAACGTATC+TGG	+	chr8.3:58252183-58252202	None:intergenic	40.0%
!!!	GAAGCGTGCTTTTTTGACAA+TGG	-	chr8.3:58252207-58252226	MS.gene88495:CDS	40.0%
	CGAACAGGAGAACAAGTTCA+AGG	-	chr8.3:58252556-58252575	MS.gene88495:intron	45.0%
	GAGCTGTTACAACAAGATCC+CGG	-	chr8.3:58252873-58252892	MS.gene88495:CDS	45.0%
	GGCAACGTTAATGAACGAAC+AGG	-	chr8.3:58252541-58252560	MS.gene88495:CDS	45.0%
!!!	GCGTGCTTTTTTGACAATGG+AGG	-	chr8.3:58252210-58252229	MS.gene88495:CDS	45.0%
	CTTGGAGAAGCAGATTGCAG+AGG	-	chr8.3:58252833-58252852	MS.gene88495:CDS	50.0%
	TGCAGGGAAAACGTAAGTCG+TGG	-	chr8.3:58252396-58252415	MS.gene88495:intron	50.0%
	GACAATGGAGGAGCTGAAGC+AGG	-	chr8.3:58252222-58252241	MS.gene88495:CDS	55.0%
	GGAGAAGCAGATTGCAGAGG+TGG	-	chr8.3:58252836-58252855	MS.gene88495:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	58252166	58252929	58252166	ID=MS.gene88495
chr8.3	mRNA	58252166	58252929	58252166	ID=MS.gene88495.t1;Parent=MS.gene88495
chr8.3	exon	58252818	58252929	58252818	ID=MS.gene88495.t1.exon1;Parent=MS.gene88495.t1
chr8.3	CDS	58252818	58252929	58252818	ID=cds.MS.gene88495.t1;Parent=MS.gene88495.t1
chr8.3	exon	58252688	58252694	58252688	ID=MS.gene88495.t1.exon2;Parent=MS.gene88495.t1
chr8.3	CDS	58252688	58252694	58252688	ID=cds.MS.gene88495.t1;Parent=MS.gene88495.t1
chr8.3	exon	58252488	58252572	58252488	ID=MS.gene88495.t1.exon3;Parent=MS.gene88495.t1
chr8.3	CDS	58252488	58252572	58252488	ID=cds.MS.gene88495.t1;Parent=MS.gene88495.t1
chr8.3	exon	58252166	58252276	58252166	ID=MS.gene88495.t1.exon4;Parent=MS.gene88495.t1
chr8.3	CDS	58252166	58252276	58252166	ID=cds.MS.gene88495.t1;Parent=MS.gene88495.t1

Gene Sequence

>MS.gene88495

ATGCAGGTGCAAACCCAGATACGTTCCAAAGAAACAGACAAGAAGCGTGCTTTTTTGACAATGGAGGAGCTGAAGCAGGTTCCTGACGATACTAATGTCTACAAATCCATTGGTATGTAAAAGATATCTTAGGAATAATCTTACAAGTATTTAGAATTGTGTTGCATATGTATTTTATTAGATGCCCTATTTTTCTTCAGAAATATAATTAATCAGTTTATTTTGGTTTTTGCAGGGAAAACGTAAGTCGTGGTTTCCTATTTAAACATGCTACAAAATAGAGAACAAAAGCTTCATTGTTGGGTTGATTAGAAAATATAAATGACATACTCTAATACAATCTTAAATTGTGGCCAGATTTGTATTGGAGACGAAGGCAACGTTAATGAACGAACAGGAGAACAAGTTCAAGGATAGTGAAACTTCAATTACCGCATTACAAGTAAATGTCCTTCCTTCACTTTTATGGAAACTTCTATTCTTTCATCATTTATTATCTTATAACTACATTGTTCTTGAATTTCTAAAGTTGCATGAAAGAAAATTATATTCAAGAATGAGACAAAAATATAACAGTTTTAGTTAAATTTCTGAATAGTAAATGAGTTTCATGATGTCTGATCTGAGTGAACAAATAATATGGCTTTGGACAGAGCTCAAAAGAATACTTGGAGAAGCAGATTGCAGAGGTGGAAAACAATTTGAGAGAGCTGTTACAACAAGATCCCGGTCTTGCTCGTCAAATTATGTCAATGAATGTGTAA

Protein sequence

>MS.gene88495.t1

MQVQTQIRSKETDKKRAFLTMEELKQVPDDTNVYKSIGKTFVLETKATLMNEQENKFKDSETSITALQSSKEYLEKQIAEVENNLRELLQQDPGLARQIMSMNV