Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016608.t1 | AET02323.2 | 99 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 1.60E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016608.t1 | Q3SZE2 | 38.1 | 84 | 52 | 0 | 6 | 89 | 33 | 116 | 7.7e-09 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016608.t1 | G7LG18 | 99.0 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 1.1e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene062939 | MS.gene016608 | PPI |
| MS.gene23366 | MS.gene016608 | PPI |
| MS.gene003631 | MS.gene016608 | PPI |
| MS.gene50691 | MS.gene016608 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016608.t1 | MTR_8g039140 | 99.038 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 5.03e-71 | 207 |
| MS.gene016608.t1 | MTR_8g039140 | 99.029 | 103 | 1 | 0 | 2 | 104 | 10 | 112 | 2.46e-70 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016608.t1 | AT2G07340 | 68.932 | 103 | 32 | 0 | 2 | 104 | 26 | 128 | 2.00e-49 | 153 |
| MS.gene016608.t1 | AT2G07340 | 68.932 | 103 | 31 | 1 | 2 | 104 | 26 | 127 | 3.54e-47 | 147 |
Find 14 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTTCTTTGGAGCGTATC+TGG | 0.131625 | 8.2:-61231665 | None:intergenic |
| ACGCTTCTTGTCTGTTTCTT+TGG | 0.240976 | 8.2:-61231676 | None:intergenic |
| AATTGTGGCCAGGTTTGTAT+TGG | 0.395225 | 8.2:+61231996 | MS.gene016608:intron |
| CATAATTTGACGAGCAAGAC+CGG | 0.429125 | 8.2:-61232377 | None:intergenic |
| TGTTTCTTTGGAGCGTATCT+GGG | 0.433824 | 8.2:-61231664 | None:intergenic |
| AGCAACTTTAATGAACGAAC+AGG | 0.462452 | 8.2:+61232026 | MS.gene016608:CDS |
| TTGTAGACATTAGTATCGTC+AGG | 0.474681 | 8.2:-61231732 | None:intergenic |
| GACAATGGAGGAGCTGAAGC+AGG | 0.474978 | 8.2:+61231707 | MS.gene016608:CDS |
| ACAGAGCTCAAAAGAATACT+TGG | 0.492644 | 8.2:+61232300 | MS.gene016608:intron |
| ACTAATGTCTACAAATCCAT+TGG | 0.553731 | 8.2:+61231741 | MS.gene016608:CDS |
| GGAGAAGCAGATTGCAGAGG+TGG | 0.574472 | 8.2:+61232321 | MS.gene016608:CDS |
| ATAATTTGACGAGCAAGACC+GGG | 0.620149 | 8.2:-61232376 | None:intergenic |
| GAGCTGTTACAACAAGATCC+CGG | 0.623013 | 8.2:+61232358 | MS.gene016608:CDS |
| CTTGGAGAAGCAGATTGCAG+AGG | 0.694862 | 8.2:+61232318 | MS.gene016608:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAATTAATCAGTTTATTT+TGG | + | chr8.2:61231854-61231873 | MS.gene016608:intron | 10.0% |
| !!! | TATATTTCTGAAGAAAAATA+GGG | - | chr8.2:61231838-61231857 | None:intergenic | 15.0% |
| !!! | TTATATTTCTGAAGAAAAAT+AGG | - | chr8.2:61231839-61231858 | None:intergenic | 15.0% |
| !!! | ATTTTGTAGCATGTTTAAAT+AGG | - | chr8.2:61231910-61231929 | None:intergenic | 20.0% |
| ! | ATCTGAGTGAACAAATAATA+TGG | + | chr8.2:61232271-61232290 | MS.gene016608:intron | 25.0% |
| ! | TCTAATACACTCTTAAATTG+TGG | + | chr8.2:61231981-61232000 | MS.gene016608:intron | 25.0% |
| ! | TGGTATGTAAAAGATATCTT+AGG | + | chr8.2:61231761-61231780 | MS.gene016608:intron | 25.0% |
| !! | AGATATCTTTTACATACCAA+TGG | - | chr8.2:61231760-61231779 | None:intergenic | 25.0% |
| !!! | AGTTTATTTTGGTTTTTGCA+GGG | + | chr8.2:61231865-61231884 | MS.gene016608:intron | 25.0% |
| ACTAATGTCTACAAATCCAT+TGG | + | chr8.2:61231741-61231760 | MS.gene016608:CDS | 30.0% | |
| AGAACAAAAGCTTCATTGTT+GGG | + | chr8.2:61231932-61231951 | MS.gene016608:intron | 30.0% | |
| AGAAGTTTCCATAAAAGTGA+AGG | - | chr8.2:61232108-61232127 | None:intergenic | 30.0% | |
| GTGAACAAATAATATGGCTT+TGG | + | chr8.2:61232277-61232296 | MS.gene016608:intron | 30.0% | |
| !! | ATGTCATTCCTTCACTTTTA+TGG | + | chr8.2:61232097-61232116 | MS.gene016608:intron | 30.0% |
| !!! | CAGTTTATTTTGGTTTTTGC+AGG | + | chr8.2:61231864-61231883 | MS.gene016608:intron | 30.0% |
| ACAGAGCTCAAAAGAATACT+TGG | + | chr8.2:61232300-61232319 | MS.gene016608:intron | 35.0% | |
| AGCAACTTTAATGAACGAAC+AGG | + | chr8.2:61232026-61232045 | MS.gene016608:CDS | 35.0% | |
| GAGAACAAAAGCTTCATTGT+TGG | + | chr8.2:61231931-61231950 | MS.gene016608:intron | 35.0% | |
| TTGTAGACATTAGTATCGTC+AGG | - | chr8.2:61231735-61231754 | None:intergenic | 35.0% | |
| AATTGTGGCCAGGTTTGTAT+TGG | + | chr8.2:61231996-61232015 | MS.gene016608:intron | 40.0% | |
| ATAATTTGACGAGCAAGACC+GGG | - | chr8.2:61232379-61232398 | None:intergenic | 40.0% | |
| CATAATTTGACGAGCAAGAC+CGG | - | chr8.2:61232380-61232399 | None:intergenic | 40.0% | |
| TACACTCTTAAATTGTGGCC+AGG | + | chr8.2:61231986-61232005 | MS.gene016608:intron | 40.0% | |
| TTTCGTCTCCAATACAAACC+TGG | - | chr8.2:61232007-61232026 | None:intergenic | 40.0% | |
| ! | ACGCTTCTTGTCTGTTTCTT+TGG | - | chr8.2:61231679-61231698 | None:intergenic | 40.0% |
| !! | TGTTTCTTTGGAGCGTATCT+GGG | - | chr8.2:61231667-61231686 | None:intergenic | 40.0% |
| !!! | GAAGCGTGCTTTTTTGACAA+TGG | + | chr8.2:61231692-61231711 | MS.gene016608:CDS | 40.0% |
| GAGCTGTTACAACAAGATCC+CGG | + | chr8.2:61232358-61232377 | MS.gene016608:CDS | 45.0% | |
| !! | CTGTTTCTTTGGAGCGTATC+TGG | - | chr8.2:61231668-61231687 | None:intergenic | 45.0% |
| !!! | GCGTGCTTTTTTGACAATGG+AGG | + | chr8.2:61231695-61231714 | MS.gene016608:CDS | 45.0% |
| CTTGGAGAAGCAGATTGCAG+AGG | + | chr8.2:61232318-61232337 | MS.gene016608:CDS | 50.0% | |
| TGCAGGGAAAACGTAAGTCG+TGG | + | chr8.2:61231881-61231900 | MS.gene016608:intron | 50.0% | |
| GACAATGGAGGAGCTGAAGC+AGG | + | chr8.2:61231707-61231726 | MS.gene016608:CDS | 55.0% | |
| GGAGAAGCAGATTGCAGAGG+TGG | + | chr8.2:61232321-61232340 | MS.gene016608:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 61231651 | 61232414 | 61231651 | ID=MS.gene016608 |
| chr8.2 | mRNA | 61231651 | 61232414 | 61231651 | ID=MS.gene016608.t1;Parent=MS.gene016608 |
| chr8.2 | exon | 61231651 | 61231762 | 61231651 | ID=MS.gene016608.t1.exon1;Parent=MS.gene016608.t1 |
| chr8.2 | CDS | 61231651 | 61231762 | 61231651 | ID=cds.MS.gene016608.t1;Parent=MS.gene016608.t1 |
| chr8.2 | exon | 61231886 | 61231892 | 61231886 | ID=MS.gene016608.t1.exon2;Parent=MS.gene016608.t1 |
| chr8.2 | CDS | 61231886 | 61231892 | 61231886 | ID=cds.MS.gene016608.t1;Parent=MS.gene016608.t1 |
| chr8.2 | exon | 61232008 | 61232092 | 61232008 | ID=MS.gene016608.t1.exon3;Parent=MS.gene016608.t1 |
| chr8.2 | CDS | 61232008 | 61232092 | 61232008 | ID=cds.MS.gene016608.t1;Parent=MS.gene016608.t1 |
| chr8.2 | exon | 61232304 | 61232414 | 61232304 | ID=MS.gene016608.t1.exon4;Parent=MS.gene016608.t1 |
| chr8.2 | CDS | 61232304 | 61232414 | 61232304 | ID=cds.MS.gene016608.t1;Parent=MS.gene016608.t1 |
| Gene Sequence |
| Protein sequence |
