Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056230.t1 | AET02323.2 | 99 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 1.60E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056230.t1 | Q3SZE2 | 38.1 | 84 | 52 | 0 | 6 | 89 | 33 | 116 | 7.7e-09 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056230.t1 | G7LG18 | 99.0 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 1.1e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene062939 | MS.gene056230 | PPI |
| MS.gene23366 | MS.gene056230 | PPI |
| MS.gene003631 | MS.gene056230 | PPI |
| MS.gene50691 | MS.gene056230 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056230.t1 | MTR_8g039140 | 99.038 | 104 | 1 | 0 | 1 | 104 | 18 | 121 | 5.03e-71 | 207 |
| MS.gene056230.t1 | MTR_8g039140 | 99.029 | 103 | 1 | 0 | 2 | 104 | 10 | 112 | 2.46e-70 | 205 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056230.t1 | AT2G07340 | 68.932 | 103 | 32 | 0 | 2 | 104 | 26 | 128 | 2.00e-49 | 153 |
| MS.gene056230.t1 | AT2G07340 | 68.932 | 103 | 31 | 1 | 2 | 104 | 26 | 127 | 3.54e-47 | 147 |
Find 15 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTTCTTTGGAGCGTATC+TGG | 0.131625 | 8.1:-65093707 | None:intergenic |
| ACGCTTCTTGTCTGTTTCTT+TGG | 0.240976 | 8.1:-65093718 | None:intergenic |
| AATTGTGGCCAGGTTTGTAT+TGG | 0.395225 | 8.1:+65094038 | MS.gene056230:intron |
| CATAATTTGACGAGCAAGAC+CGG | 0.429125 | 8.1:-65094419 | None:intergenic |
| TGTTTCTTTGGAGCGTATCT+GGG | 0.433824 | 8.1:-65093706 | None:intergenic |
| AGCAACTTTAATGAACGAAC+AGG | 0.462452 | 8.1:+65094068 | MS.gene056230:CDS |
| TTGTAGACATTAGTATCGTC+AGG | 0.474681 | 8.1:-65093774 | None:intergenic |
| GACAATGGAGGAGCTGAAGC+AGG | 0.474978 | 8.1:+65093749 | MS.gene056230:CDS |
| ACAGAGCTCAAAAGAATACT+TGG | 0.492644 | 8.1:+65094342 | MS.gene056230:intron |
| CGAACAGGAGAACAAGTTCA+AGG | 0.550157 | 8.1:+65094083 | MS.gene056230:CDS |
| ACTAATGTCTACAAATCCAT+TGG | 0.553731 | 8.1:+65093783 | MS.gene056230:CDS |
| GGAGAAGCAGATTGCAGAGG+TGG | 0.574472 | 8.1:+65094363 | MS.gene056230:CDS |
| ATAATTTGACGAGCAAGACC+GGG | 0.620149 | 8.1:-65094418 | None:intergenic |
| GAGCTGTTACAACAAGATCC+CGG | 0.623013 | 8.1:+65094400 | MS.gene056230:CDS |
| CTTGGAGAAGCAGATTGCAG+AGG | 0.694862 | 8.1:+65094360 | MS.gene056230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAATTAAACAGTTTATTT+TGG | + | chr8.1:65093896-65093915 | MS.gene056230:intron | 10.0% |
| !!! | TATATTTCTGAAGAAAAATA+GGG | - | chr8.1:65093880-65093899 | None:intergenic | 15.0% |
| !!! | TTATATTTCTGAAGAAAAAT+AGG | - | chr8.1:65093881-65093900 | None:intergenic | 15.0% |
| !!! | ATTTTGTAGCATGTTTAAAT+AGG | - | chr8.1:65093952-65093971 | None:intergenic | 20.0% |
| ! | ATCTGAGTGAACAAATAATA+TGG | + | chr8.1:65094313-65094332 | MS.gene056230:intron | 25.0% |
| ! | TCTAATACACTCTTAAATTG+TGG | + | chr8.1:65094023-65094042 | MS.gene056230:intron | 25.0% |
| ! | TGGTATGTAAAAGATATCTT+AGG | + | chr8.1:65093803-65093822 | MS.gene056230:intron | 25.0% |
| !! | AGATATCTTTTACATACCAA+TGG | - | chr8.1:65093802-65093821 | None:intergenic | 25.0% |
| !!! | AGTTTATTTTGGTTTTTGCA+GGG | + | chr8.1:65093907-65093926 | MS.gene056230:intron | 25.0% |
| ACTAATGTCTACAAATCCAT+TGG | + | chr8.1:65093783-65093802 | MS.gene056230:CDS | 30.0% | |
| AGAACAAAAGCTTCATTGTT+GGG | + | chr8.1:65093974-65093993 | MS.gene056230:intron | 30.0% | |
| AGAAGTTTCCATAAAAGTGA+AGG | - | chr8.1:65094150-65094169 | None:intergenic | 30.0% | |
| GTGAACAAATAATATGGCTT+TGG | + | chr8.1:65094319-65094338 | MS.gene056230:intron | 30.0% | |
| !! | ATGTCATTCCTTCACTTTTA+TGG | + | chr8.1:65094139-65094158 | MS.gene056230:intron | 30.0% |
| !!! | CAGTTTATTTTGGTTTTTGC+AGG | + | chr8.1:65093906-65093925 | MS.gene056230:intron | 30.0% |
| ACAGAGCTCAAAAGAATACT+TGG | + | chr8.1:65094342-65094361 | MS.gene056230:intron | 35.0% | |
| AGCAACTTTAATGAACGAAC+AGG | + | chr8.1:65094068-65094087 | MS.gene056230:CDS | 35.0% | |
| GAGAACAAAAGCTTCATTGT+TGG | + | chr8.1:65093973-65093992 | MS.gene056230:intron | 35.0% | |
| TTGTAGACATTAGTATCGTC+AGG | - | chr8.1:65093777-65093796 | None:intergenic | 35.0% | |
| AATTGTGGCCAGGTTTGTAT+TGG | + | chr8.1:65094038-65094057 | MS.gene056230:intron | 40.0% | |
| ATAATTTGACGAGCAAGACC+GGG | - | chr8.1:65094421-65094440 | None:intergenic | 40.0% | |
| CATAATTTGACGAGCAAGAC+CGG | - | chr8.1:65094422-65094441 | None:intergenic | 40.0% | |
| TACACTCTTAAATTGTGGCC+AGG | + | chr8.1:65094028-65094047 | MS.gene056230:intron | 40.0% | |
| TTTCGTCTCCAATACAAACC+TGG | - | chr8.1:65094049-65094068 | None:intergenic | 40.0% | |
| ! | ACGCTTCTTGTCTGTTTCTT+TGG | - | chr8.1:65093721-65093740 | None:intergenic | 40.0% |
| !! | TGTTTCTTTGGAGCGTATCT+GGG | - | chr8.1:65093709-65093728 | None:intergenic | 40.0% |
| !!! | GAAGCGTGCTTTTTTGACAA+TGG | + | chr8.1:65093734-65093753 | MS.gene056230:CDS | 40.0% |
| CGAACAGGAGAACAAGTTCA+AGG | + | chr8.1:65094083-65094102 | MS.gene056230:CDS | 45.0% | |
| GAGCTGTTACAACAAGATCC+CGG | + | chr8.1:65094400-65094419 | MS.gene056230:CDS | 45.0% | |
| !! | CTGTTTCTTTGGAGCGTATC+TGG | - | chr8.1:65093710-65093729 | None:intergenic | 45.0% |
| !!! | GCGTGCTTTTTTGACAATGG+AGG | + | chr8.1:65093737-65093756 | MS.gene056230:CDS | 45.0% |
| CTTGGAGAAGCAGATTGCAG+AGG | + | chr8.1:65094360-65094379 | MS.gene056230:CDS | 50.0% | |
| TGCAGGGAAAACGTAAGTCG+TGG | + | chr8.1:65093923-65093942 | MS.gene056230:intron | 50.0% | |
| GACAATGGAGGAGCTGAAGC+AGG | + | chr8.1:65093749-65093768 | MS.gene056230:CDS | 55.0% | |
| GGAGAAGCAGATTGCAGAGG+TGG | + | chr8.1:65094363-65094382 | MS.gene056230:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 65093693 | 65094456 | 65093693 | ID=MS.gene056230 |
| chr8.1 | mRNA | 65093693 | 65094456 | 65093693 | ID=MS.gene056230.t1;Parent=MS.gene056230 |
| chr8.1 | exon | 65093693 | 65093804 | 65093693 | ID=MS.gene056230.t1.exon1;Parent=MS.gene056230.t1 |
| chr8.1 | CDS | 65093693 | 65093804 | 65093693 | ID=cds.MS.gene056230.t1;Parent=MS.gene056230.t1 |
| chr8.1 | exon | 65093928 | 65093934 | 65093928 | ID=MS.gene056230.t1.exon2;Parent=MS.gene056230.t1 |
| chr8.1 | CDS | 65093928 | 65093934 | 65093928 | ID=cds.MS.gene056230.t1;Parent=MS.gene056230.t1 |
| chr8.1 | exon | 65094050 | 65094134 | 65094050 | ID=MS.gene056230.t1.exon3;Parent=MS.gene056230.t1 |
| chr8.1 | CDS | 65094050 | 65094134 | 65094050 | ID=cds.MS.gene056230.t1;Parent=MS.gene056230.t1 |
| chr8.1 | exon | 65094346 | 65094456 | 65094346 | ID=MS.gene056230.t1.exon4;Parent=MS.gene056230.t1 |
| chr8.1 | CDS | 65094346 | 65094456 | 65094346 | ID=cds.MS.gene056230.t1;Parent=MS.gene056230.t1 |
| Gene Sequence |
| Protein sequence |
