AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00366

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00366.t1 MTR_2g437700 98.681 379 4 1 1 379 12 389 0.0 737
MS.gene00366.t1 MTR_7g075453 32.759 348 214 11 16 361 23 352 6.52e-37 138
MS.gene00366.t1 MTR_2g018340 41.558 154 84 3 182 332 37 187 1.33e-31 119
MS.gene00366.t1 MTR_3g460780 28.840 319 213 10 16 333 31 336 9.25e-26 107
MS.gene00366.t1 MTR_5g098420 27.726 321 212 11 16 333 35 338 6.41e-22 97.1
MS.gene00366.t1 MTR_7g101080 31.677 161 101 4 179 335 35 190 4.45e-17 80.5
MS.gene00366.t1 MTR_5g098060 34.615 182 106 5 182 360 46 217 5.70e-17 80.5
MS.gene00366.t1 MTR_2g018380 36.066 122 72 3 230 345 53 174 1.40e-16 77.8
MS.gene00366.t1 MTR_4g059840 32.240 183 110 6 183 360 72 245 4.41e-15 75.5
MS.gene00366.t1 MTR_2g093980 29.221 154 102 3 184 334 69 218 2.36e-14 73.2
MS.gene00366.t1 MTR_2g017950 27.749 191 125 4 183 364 70 256 3.04e-14 73.2
MS.gene00366.t1 MTR_4g053380 31.667 180 110 6 183 355 76 249 3.92e-14 72.8
MS.gene00366.t1 MTR_4g059790 30.387 181 116 5 183 360 72 245 4.28e-14 72.4
MS.gene00366.t1 MTR_8g087460 30.769 156 97 4 183 332 92 242 4.97e-14 72.8
MS.gene00366.t1 MTR_8g087470 30.769 156 97 4 183 332 92 242 5.56e-14 72.4
MS.gene00366.t1 MTR_2g090765 29.730 185 121 5 153 334 8 186 9.41e-13 67.8
MS.gene00366.t1 MTR_8g087450 31.544 149 95 4 184 329 77 221 1.35e-12 68.2
MS.gene00366.t1 MTR_2g093970 26.943 193 120 5 184 359 69 257 2.99e-12 67.0
MS.gene00366.t1 MTR_4g059720 27.778 162 108 4 174 333 35 189 3.89e-12 66.2
MS.gene00366.t1 MTR_2g017970 29.213 178 117 6 184 358 68 239 5.83e-12 66.2
MS.gene00366.t1 MTR_2g017960 29.213 178 117 6 184 358 68 239 8.22e-12 65.5
MS.gene00366.t1 MTR_2g093990 28.947 152 102 3 184 333 41 188 1.18e-11 64.7
MS.gene00366.t1 MTR_1g009950 28.161 174 114 5 14 184 23 188 2.67e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00366.t1 AT3G46550 55.202 346 137 5 15 346 29 370 1.15e-119 353
MS.gene00366.t1 AT2G45470 33.333 324 202 10 14 336 25 335 1.91e-35 134
MS.gene00366.t1 AT3G60900 32.471 348 213 11 16 354 27 361 6.49e-31 122
MS.gene00366.t1 AT5G55730 29.538 325 213 11 10 333 20 329 9.71e-26 107
MS.gene00366.t1 AT5G55730 29.538 325 213 11 10 333 20 329 9.71e-26 107
MS.gene00366.t1 AT4G12730 28.358 335 223 12 1 333 13 332 6.35e-24 102
MS.gene00366.t1 AT5G44130 29.798 198 132 5 183 378 35 227 4.42e-18 83.2
MS.gene00366.t1 AT5G60490 31.579 152 99 3 184 333 39 187 3.09e-17 80.9
MS.gene00366.t1 AT1G03870 29.282 181 119 4 183 359 38 213 2.43e-15 75.5
MS.gene00366.t1 AT2G20520 31.646 158 97 5 183 334 37 189 3.55e-14 72.0
MS.gene00366.t1 AT5G03170 33.962 159 99 4 179 334 31 186 1.53e-13 70.1
MS.gene00366.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.43e-13 69.7
MS.gene00366.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.43e-13 69.7
MS.gene00366.t1 AT3G12660 31.210 157 103 3 10 166 19 170 4.42e-13 68.9

Find 146 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAATGGTGTTGGTGTTC+GGG 0.251448 2.1:+55702480 None:intergenic
GTGAGCATCGATTAGGGTTT+TGG 0.254061 2.1:+55702295 None:intergenic
GATTAACACTGGCATTGTTC+AGG 0.317584 2.1:-55701958 MS.gene00366:CDS
TTGTTGCCAAGAGAGATATT+TGG 0.326646 2.1:-55701873 MS.gene00366:CDS
GAGGTGAATGTTGATAATTC+AGG 0.334884 2.1:+55702779 None:intergenic
GGTCGGACCAGGAGAGGAAT+TGG 0.344781 2.1:+55702600 None:intergenic
TGAAATTGTGAGCATCGATT+AGG 0.344995 2.1:+55702288 None:intergenic
CTTTCTTCACCGCCAGGGTT+TGG 0.356296 2.1:-55701744 MS.gene00366:CDS
GCTTCAATGCTCTCCGCTTC+CGG 0.361932 2.1:-55702257 MS.gene00366:CDS
GTGAGGGTCGGTGGTGAGAT+AGG 0.366350 2.1:+55702679 None:intergenic
TGTTGCCAAGAGAGATATTT+GGG 0.370591 2.1:-55701872 MS.gene00366:CDS
GATGTCAGCGAGGGCGGAGG+GGG 0.371105 2.1:+55702643 None:intergenic
ATGGTGTTGGGGATAGGATT+AGG 0.379163 2.1:+55702840 None:intergenic
ATTGTAATGGTGTTGGTGTT+CGG 0.388000 2.1:+55702479 None:intergenic
GAAATTGTGAGCATCGATTA+GGG 0.392929 2.1:+55702289 None:intergenic
AGAGGAATTGGAGGAGGATG+TGG 0.392932 2.1:+55702612 None:intergenic
TGGTGGCCCGGCCGGTGGTT+TGG 0.397807 2.1:+55702534 None:intergenic
TCCTCCTCCAATTCCTCTCC+TGG 0.408759 2.1:-55702607 MS.gene00366:CDS
TGAAAGAGAAAGATGGTGTT+GGG 0.411336 2.1:+55702828 None:intergenic
GGAGATTTCGCAGACACAAT+TGG 0.413692 2.1:+55701843 None:intergenic
CTTCGTGCTTATTATCCTCT+CGG 0.417892 2.1:-55702086 MS.gene00366:CDS
GGCCGGGCCACCAACAACTT+TGG 0.425079 2.1:-55702524 MS.gene00366:CDS
GTCGGTGGTGAGATAGGTGT+TGG 0.428158 2.1:+55702685 None:intergenic
AAGAGTAAGGTGCTGGGGAA+TGG 0.428506 2.1:+55702456 None:intergenic
GAGGATGTCAGCGAGGGCGG+AGG 0.433140 2.1:+55702640 None:intergenic
GGGGAGAGGTTATGGAGGTG+AGG 0.434679 2.1:+55702662 None:intergenic
TTCTTCCCAAATATCTCTCT+TGG 0.439416 2.1:+55701867 None:intergenic
TGAAAGAACGGTGGCGTTTG+AGG 0.443446 2.1:+55702433 None:intergenic
GAAGCCGGTGAAGGTGGTTC+CGG 0.446950 2.1:-55702218 MS.gene00366:CDS
GAGGTGTTGGAGGTCGGACC+AGG 0.448627 2.1:+55702589 None:intergenic
AGAAAGATGGTGTTGGGGAT+AGG 0.453694 2.1:+55702834 None:intergenic
GAGAGTGGTGACGAGTTTAC+CGG 0.456170 2.1:+55702559 None:intergenic
TCGGCGAAAGCATCGTCTAC+GGG 0.459267 2.1:+55702179 None:intergenic
GGATGTCAGCGAGGGCGGAG+GGG 0.461161 2.1:+55702642 None:intergenic
CGGAGGGAGGGAGGTGTTGG+AGG 0.464496 2.1:+55702579 None:intergenic
CCCTTACGGTTTCGATCTCA+TGG 0.465462 2.1:-55702354 MS.gene00366:CDS
GATGCTGCTTCTTCGCCGGC+GGG 0.465731 2.1:-55701789 MS.gene00366:CDS
ATTAACACTGGCATTGTTCA+GGG 0.469145 2.1:-55701957 MS.gene00366:CDS
GGTGAAGATAGTGAGATTGT+AGG 0.469199 2.1:+55702394 None:intergenic
GACACTTGCGACGGAAGCTA+TGG 0.469462 2.1:-55702030 MS.gene00366:CDS
TCACATCTTTCTTCACCGCC+AGG 0.471478 2.1:-55701750 MS.gene00366:CDS
CTCTCTTCCAAACCACCGGC+CGG 0.475597 2.1:-55702541 MS.gene00366:CDS
TTCAGGGAAGGTTGAAAGGA+GGG 0.476637 2.1:+55702796 None:intergenic
AGGTGAATGTTGATAATTCA+GGG 0.477819 2.1:+55702780 None:intergenic
AGCCAAAGTTGTTGGTGGCC+CGG 0.478504 2.1:+55702522 None:intergenic
ATTGGAGGAGGATGTGGTAG+CGG 0.481178 2.1:+55702618 None:intergenic
GGGAGAGATCAGCGGTGAGA+GGG 0.486859 2.1:+55702738 None:intergenic
GAAACACCGAAAATCGCAAT+AGG 0.488328 2.1:+55701903 None:intergenic
TAGTGCTCCTCCTCCTTATG+AGG 0.491845 2.1:-55701811 MS.gene00366:CDS
TCGGTGGTGAGATAGGTGTT+GGG 0.492560 2.1:+55702686 None:intergenic
TTTGAGGAAGAGTAAGGTGC+TGG 0.494680 2.1:+55702449 None:intergenic
AAAGTTGTTGGTGGCCCGGC+CGG 0.495578 2.1:+55702526 None:intergenic
TACCGGAGGGAGGGAGGTGT+TGG 0.497410 2.1:+55702576 None:intergenic
TTCATTCACGACGCCGGAAG+CGG 0.499715 2.1:+55702244 None:intergenic
GAAGAAGCAGCATCCTCATA+AGG 0.500145 2.1:+55701798 None:intergenic
GTTGTTGGTGGCCCGGCCGG+TGG 0.501570 2.1:+55702529 None:intergenic
AGGATGTCAGCGAGGGCGGA+GGG 0.513684 2.1:+55702641 None:intergenic
GTCGCAAGTGTCGGTTGCAA+CGG 0.515529 2.1:+55702041 None:intergenic
AGGTTGAAAGGAGGGAGGTG+AGG 0.516160 2.1:+55702804 None:intergenic
ATAATTCAGGGAAGGTTGAA+AGG 0.519692 2.1:+55702792 None:intergenic
ATCGGCGAAAGCATCGTCTA+CGG 0.521274 2.1:+55702178 None:intergenic
GGGGATAAACAAGGTGATAC+CGG 0.523824 2.1:+55702199 None:intergenic
CTCCAACACCTCCCTCCCTC+CGG 0.523989 2.1:-55702578 MS.gene00366:CDS
TTGAAAGAGAAAGATGGTGT+TGG 0.524791 2.1:+55702827 None:intergenic
GCGGAGGATGTCAGCGAGGG+CGG 0.525244 2.1:+55702637 None:intergenic
CACATCTTTCTTCACCGCCA+GGG 0.525421 2.1:-55701749 MS.gene00366:CDS
GCGACGGAAGCTATGGGTGC+TGG 0.525814 2.1:-55702023 MS.gene00366:CDS
GTTATGGAGGTGAGGGTCGG+TGG 0.527291 2.1:+55702670 None:intergenic
GATACCGGAACCACCTTCAC+CGG 0.527970 2.1:+55702214 None:intergenic
GTGAAGATAGTGAGATTGTA+GGG 0.528446 2.1:+55702395 None:intergenic
GGGAGGGAGGTGTTGGAGGT+CGG 0.529487 2.1:+55702583 None:intergenic
CGGACCAGGAGAGGAATTGG+AGG 0.529498 2.1:+55702603 None:intergenic
GGAGGAGGAGCACTACTCTC+CGG 0.529688 2.1:+55701819 None:intergenic
ATTCAGGGAAGGTTGAAAGG+AGG 0.530201 2.1:+55702795 None:intergenic
TGGGAGAGATCAGCGGTGAG+AGG 0.533298 2.1:+55702737 None:intergenic
TCTCTTCCAAACCACCGGCC+GGG 0.536880 2.1:-55702540 MS.gene00366:CDS
AATGAATTCGAAGCCGGTGA+AGG 0.538116 2.1:-55702227 MS.gene00366:CDS
GGAGAGAGAGTGAATTGCGG+TGG 0.541485 2.1:+55702717 None:intergenic
GGAATGGATTGTAATGGTGT+TGG 0.543280 2.1:+55702472 None:intergenic
GTTGACGGAGCCAAAGTTGT+TGG 0.548182 2.1:+55702514 None:intergenic
GGGAGAGGTTATGGAGGTGA+GGG 0.552715 2.1:+55702663 None:intergenic
TGCTGGGGAATGGATTGTAA+TGG 0.553637 2.1:+55702466 None:intergenic
GGATGCTGCTTCTTCGCCGG+CGG 0.553952 2.1:-55701790 MS.gene00366:CDS
TGTAATGGTGTTGGTGTTCG+GGG 0.554234 2.1:+55702481 None:intergenic
CGGTTTCGATCTCATGGCAT+CGG 0.555806 2.1:-55702348 MS.gene00366:CDS
TTCGAATTCATTCACGACGC+CGG 0.555834 2.1:+55702238 None:intergenic
GTGATGTTGAGAAGAATGCT+TGG 0.555902 2.1:+55702317 None:intergenic
GCCATGAGATCGAAACCGTA+AGG 0.558245 2.1:+55702353 None:intergenic
GTGGCGTTTGAGGAAGAGTA+AGG 0.559294 2.1:+55702443 None:intergenic
GAGGTTATGGAGGTGAGGGT+CGG 0.559779 2.1:+55702667 None:intergenic
GTGAGGTTGAAAGAGAAAGA+TGG 0.560128 2.1:+55702821 None:intergenic
TGAGGATGCTGCTTCTTCGC+CGG 0.566290 2.1:-55701793 MS.gene00366:CDS
ACCAGGAGAGGAATTGGAGG+AGG 0.566516 2.1:+55702606 None:intergenic
CGAAACGGAAGGAGGTAGAT+CGG 0.567781 2.1:+55702160 None:intergenic
TTGAGGAAGAGTAAGGTGCT+GGG 0.571039 2.1:+55702450 None:intergenic
GAGAGATCAGCGGTGAGAGG+GGG 0.573409 2.1:+55702740 None:intergenic
GGAGAGATCAGCGGTGAGAG+GGG 0.577869 2.1:+55702739 None:intergenic
GAATTCGAAGCCGGTGAAGG+TGG 0.580678 2.1:-55702224 MS.gene00366:CDS
GACGGAGCCAAAGTTGTTGG+TGG 0.580994 2.1:+55702517 None:intergenic
GGCTCTGTTGCGATTAACAC+TGG 0.583502 2.1:-55701969 MS.gene00366:CDS
GAGAGAGAGTGAATTGCGGT+GGG 0.591977 2.1:+55702718 None:intergenic
GGAGGAGGATGTGGTAGCGG+AGG 0.593191 2.1:+55702621 None:intergenic
ACACTTGCGACGGAAGCTAT+GGG 0.597246 2.1:-55702029 MS.gene00366:CDS
GGGAAGTGATTGAAGCGAAA+CGG 0.597351 2.1:+55702145 None:intergenic
ACCACTCTCTTCCAAACCAC+CGG 0.598222 2.1:-55702545 MS.gene00366:CDS
AGGGATTAAAAGAGAATCAA+CGG 0.602507 2.1:+55702373 None:intergenic
GAATGTTGATAATTCAGGGA+AGG 0.603611 2.1:+55702784 None:intergenic
ATAGCTTCCGTCGCAAGTGT+CGG 0.604769 2.1:+55702032 None:intergenic
GGTGAGATAGGTGTTGGGGA+CGG 0.604958 2.1:+55702691 None:intergenic
TGACGAGTTTACCGGAGGGA+GGG 0.605757 2.1:+55702567 None:intergenic
GCAGCATCCTCATAAGGAGG+AGG 0.606713 2.1:+55701804 None:intergenic
CGGGGAGTGAGTGATGTTGA+CGG 0.609852 2.1:+55702499 None:intergenic
CGAGTTTACCGGAGGGAGGG+AGG 0.611461 2.1:+55702570 None:intergenic
TGTGTCTGCGAAATCTCCAC+CGG 0.612137 2.1:-55701838 MS.gene00366:CDS
AGTGATTGAAGCGAAACGGA+AGG 0.612745 2.1:+55702149 None:intergenic
CGGTTGCAACGGATTCGCAG+TGG 0.617668 2.1:+55702052 None:intergenic
AGGGAAGGTTGAAAGGAGGG+AGG 0.618290 2.1:+55702799 None:intergenic
CGGTGGTGAGATAGGTGTTG+GGG 0.618735 2.1:+55702687 None:intergenic
GTCGTGAATGAATTCGAAGC+CGG 0.623460 2.1:-55702233 MS.gene00366:CDS
GGCTGTACATCAAAACCCGC+CGG 0.624496 2.1:+55701774 None:intergenic
GCCGGTGGTTTGGAAGAGAG+TGG 0.626248 2.1:+55702544 None:intergenic
CGGCGAAAGCATCGTCTACG+GGG 0.626762 2.1:+55702180 None:intergenic
TTAACACTGGCATTGTTCAG+GGG 0.631498 2.1:-55701956 MS.gene00366:CDS
GTGACGAGTTTACCGGAGGG+AGG 0.632618 2.1:+55702566 None:intergenic
CCATGAGATCGAAACCGTAA+GGG 0.633243 2.1:+55702354 None:intergenic
GAAAGAGAAAGATGGTGTTG+GGG 0.638474 2.1:+55702829 None:intergenic
GGAGGAGCACTACTCTCCGG+TGG 0.642609 2.1:+55701822 None:intergenic
AGTGGTGACGAGTTTACCGG+AGG 0.642661 2.1:+55702562 None:intergenic
GTTGGAGGTCGGACCAGGAG+AGG 0.643631 2.1:+55702594 None:intergenic
GGGTGTGGAAGAAAGTAGGG+AGG 0.644752 2.1:+55702760 None:intergenic
ATCGTCTACGGGGATAAACA+AGG 0.645330 2.1:+55702190 None:intergenic
GTAGCGGAGGATGTCAGCGA+GGG 0.649494 2.1:+55702634 None:intergenic
GTGGATTGAAGCGAACCGAG+AGG 0.652533 2.1:+55702071 None:intergenic
CTAACATCTTCACCAAACCC+TGG 0.655235 2.1:+55701732 None:intergenic
TGAGGAAGAGTAAGGTGCTG+GGG 0.655459 2.1:+55702451 None:intergenic
GGCGGTGAAGAAAGATGTGA+CGG 0.657561 2.1:+55701753 None:intergenic
GAAGCAGCATCCTCATAAGG+AGG 0.663333 2.1:+55701801 None:intergenic
GTGGTGACGAGTTTACCGGA+GGG 0.663417 2.1:+55702563 None:intergenic
GATAGGTGTTGGGGACGGCG+AGG 0.663521 2.1:+55702696 None:intergenic
GATTGAAGCGAAACGGAAGG+AGG 0.667934 2.1:+55702152 None:intergenic
GTTGCAACCGACACTTGCGA+CGG 0.682389 2.1:-55702039 MS.gene00366:CDS
CGAGGAGAGAGAGTGAATTG+CGG 0.703819 2.1:+55702714 None:intergenic
ATTGCGGTGGGAGAGATCAG+CGG 0.710115 2.1:+55702730 None:intergenic
GGTAGCGGAGGATGTCAGCG+AGG 0.710162 2.1:+55702633 None:intergenic
TTTGAGCTGTGAAAGAACGG+TGG 0.719224 2.1:+55702424 None:intergenic
ACATCTTCACCAAACCCTGG+CGG 0.727699 2.1:+55701735 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATATTTCAACTTTTAA+TGG - chr2.1:55702507-55702526 MS.gene00366:CDS 15.0%
AGGGATTAAAAGAGAATCAA+CGG + chr2.1:55702127-55702146 None:intergenic 30.0%
! GATTCTCTTTTAATCCCTTA+CGG - chr2.1:55702129-55702148 MS.gene00366:CDS 30.0%
!! AGGTGAATGTTGATAATTCA+GGG + chr2.1:55701720-55701739 None:intergenic 30.0%
!!! AAGAACAACTGCTTTTTGAT+CGG + chr2.1:55702379-55702398 None:intergenic 30.0%
ATAATTCAGGGAAGGTTGAA+AGG + chr2.1:55701708-55701727 None:intergenic 35.0%
ATTAACACTGGCATTGTTCA+GGG - chr2.1:55702540-55702559 MS.gene00366:CDS 35.0%
GTGAAGATAGTGAGATTGTA+GGG + chr2.1:55702105-55702124 None:intergenic 35.0%
TCAGATGTTGCAAGTGTTTA+TGG - chr2.1:55702789-55702808 MS.gene00366:CDS 35.0%
TGAAAGAGAAAGATGGTGTT+GGG + chr2.1:55701672-55701691 None:intergenic 35.0%
TGTTGCCAAGAGAGATATTT+GGG - chr2.1:55702625-55702644 MS.gene00366:CDS 35.0%
TTCTTCCCAAATATCTCTCT+TGG + chr2.1:55702633-55702652 None:intergenic 35.0%
TTGAAAGAGAAAGATGGTGT+TGG + chr2.1:55701673-55701692 None:intergenic 35.0%
TTGTTGCCAAGAGAGATATT+TGG - chr2.1:55702624-55702643 MS.gene00366:CDS 35.0%
! CAGAATCCTATTGCGATTTT+CGG - chr2.1:55702588-55702607 MS.gene00366:CDS 35.0%
! CATTTTGAGCTGTGAAAGAA+CGG + chr2.1:55702079-55702098 None:intergenic 35.0%
!! ATTGTAATGGTGTTGGTGTT+CGG + chr2.1:55702021-55702040 None:intergenic 35.0%
!! GAAATTGTGAGCATCGATTA+GGG + chr2.1:55702211-55702230 None:intergenic 35.0%
!! GAATGTTGATAATTCAGGGA+AGG + chr2.1:55701716-55701735 None:intergenic 35.0%
!! GAGGTGAATGTTGATAATTC+AGG + chr2.1:55701721-55701740 None:intergenic 35.0%
!! TGAAATTGTGAGCATCGATT+AGG + chr2.1:55702212-55702231 None:intergenic 35.0%
CTTCGTGCTTATTATCCTCT+CGG - chr2.1:55702411-55702430 MS.gene00366:CDS 40.0%
GAAACACCGAAAATCGCAAT+AGG + chr2.1:55702597-55702616 None:intergenic 40.0%
GATTAACACTGGCATTGTTC+AGG - chr2.1:55702539-55702558 MS.gene00366:CDS 40.0%
GGTGAAGATAGTGAGATTGT+AGG + chr2.1:55702106-55702125 None:intergenic 40.0%
GTGAGGTTGAAAGAGAAAGA+TGG + chr2.1:55701679-55701698 None:intergenic 40.0%
GTGATGTTGAGAAGAATGCT+TGG + chr2.1:55702183-55702202 None:intergenic 40.0%
TTAACACTGGCATTGTTCAG+GGG - chr2.1:55702541-55702560 MS.gene00366:CDS 40.0%
! GAAAGAGAAAGATGGTGTTG+GGG + chr2.1:55701671-55701690 None:intergenic 40.0%
!! GGAATGGATTGTAATGGTGT+TGG + chr2.1:55702028-55702047 None:intergenic 40.0%
!! TTGTAATGGTGTTGGTGTTC+GGG + chr2.1:55702020-55702039 None:intergenic 40.0%
!!! AACAACTGCTTTTTGATCGG+CGG + chr2.1:55702376-55702395 None:intergenic 40.0%
AATGAATTCGAAGCCGGTGA+AGG - chr2.1:55702270-55702289 MS.gene00366:CDS 45.0%
AGTGATTGAAGCGAAACGGA+AGG + chr2.1:55702351-55702370 None:intergenic 45.0%
ATCGTCTACGGGGATAAACA+AGG + chr2.1:55702310-55702329 None:intergenic 45.0%
ATTCAGGGAAGGTTGAAAGG+AGG + chr2.1:55701705-55701724 None:intergenic 45.0%
CCATGAGATCGAAACCGTAA+GGG + chr2.1:55702146-55702165 None:intergenic 45.0%
CTAACATCTTCACCAAACCC+TGG + chr2.1:55702768-55702787 None:intergenic 45.0%
GAAGAAGCAGCATCCTCATA+AGG + chr2.1:55702702-55702721 None:intergenic 45.0%
GGAGATTTCGCAGACACAAT+TGG + chr2.1:55702657-55702676 None:intergenic 45.0%
GGGAAGTGATTGAAGCGAAA+CGG + chr2.1:55702355-55702374 None:intergenic 45.0%
GGGGATAAACAAGGTGATAC+CGG + chr2.1:55702301-55702320 None:intergenic 45.0%
GTCGTGAATGAATTCGAAGC+CGG - chr2.1:55702264-55702283 MS.gene00366:CDS 45.0%
TTCAGGGAAGGTTGAAAGGA+GGG + chr2.1:55701704-55701723 None:intergenic 45.0%
TTCGAATTCATTCACGACGC+CGG + chr2.1:55702262-55702281 None:intergenic 45.0%
TTTGAGCTGTGAAAGAACGG+TGG + chr2.1:55702076-55702095 None:intergenic 45.0%
TTTGAGGAAGAGTAAGGTGC+TGG + chr2.1:55702051-55702070 None:intergenic 45.0%
! AGAAAGATGGTGTTGGGGAT+AGG + chr2.1:55701666-55701685 None:intergenic 45.0%
! ATGGTGTTGGGGATAGGATT+AGG + chr2.1:55701660-55701679 None:intergenic 45.0%
! TTGAGGAAGAGTAAGGTGCT+GGG + chr2.1:55702050-55702069 None:intergenic 45.0%
!! TGCTGGGGAATGGATTGTAA+TGG + chr2.1:55702034-55702053 None:intergenic 45.0%
!! TGTAATGGTGTTGGTGTTCG+GGG + chr2.1:55702019-55702038 None:intergenic 45.0%
!!! ACAACTGCTTTTTGATCGGC+GGG + chr2.1:55702375-55702394 None:intergenic 45.0%
!!! GTGAGCATCGATTAGGGTTT+TGG + chr2.1:55702205-55702224 None:intergenic 45.0%
!!! TGCGATTTTCGGTGTTTCGA+AGG - chr2.1:55702599-55702618 MS.gene00366:CDS 45.0%
ACACTTGCGACGGAAGCTAT+GGG - chr2.1:55702468-55702487 MS.gene00366:CDS 50.0%
ACATCTTCACCAAACCCTGG+CGG + chr2.1:55702765-55702784 None:intergenic 50.0%
ACCACTCTCTTCCAAACCAC+CGG - chr2.1:55701952-55701971 MS.gene00366:CDS 50.0%
AGAGGAATTGGAGGAGGATG+TGG + chr2.1:55701888-55701907 None:intergenic 50.0%
ATAGCTTCCGTCGCAAGTGT+CGG + chr2.1:55702468-55702487 None:intergenic 50.0%
ATTGGAGGAGGATGTGGTAG+CGG + chr2.1:55701882-55701901 None:intergenic 50.0%
CACATCTTTCTTCACCGCCA+GGG - chr2.1:55702748-55702767 MS.gene00366:CDS 50.0%
CCCTTACGGTTTCGATCTCA+TGG - chr2.1:55702143-55702162 MS.gene00366:CDS 50.0%
CGAAACGGAAGGAGGTAGAT+CGG + chr2.1:55702340-55702359 None:intergenic 50.0%
CGAGGAGAGAGAGTGAATTG+CGG + chr2.1:55701786-55701805 None:intergenic 50.0%
CGGTTTCGATCTCATGGCAT+CGG - chr2.1:55702149-55702168 MS.gene00366:CDS 50.0%
GAAGCAGCATCCTCATAAGG+AGG + chr2.1:55702699-55702718 None:intergenic 50.0%
GAGAGAGAGTGAATTGCGGT+GGG + chr2.1:55701782-55701801 None:intergenic 50.0%
GATTGAAGCGAAACGGAAGG+AGG + chr2.1:55702348-55702367 None:intergenic 50.0%
GCCATGAGATCGAAACCGTA+AGG + chr2.1:55702147-55702166 None:intergenic 50.0%
GGCGGTGAAGAAAGATGTGA+CGG + chr2.1:55702747-55702766 None:intergenic 50.0%
GGCTCTGTTGCGATTAACAC+TGG - chr2.1:55702528-55702547 MS.gene00366:CDS 50.0%
GTGGCGTTTGAGGAAGAGTA+AGG + chr2.1:55702057-55702076 None:intergenic 50.0%
GTTGACGGAGCCAAAGTTGT+TGG + chr2.1:55701986-55702005 None:intergenic 50.0%
TAGTGCTCCTCCTCCTTATG+AGG - chr2.1:55702686-55702705 MS.gene00366:CDS 50.0%
TCACATCTTTCTTCACCGCC+AGG - chr2.1:55702747-55702766 MS.gene00366:CDS 50.0%
TCGGTGGTGAGATAGGTGTT+GGG + chr2.1:55701814-55701833 None:intergenic 50.0%
TGAAAGAACGGTGGCGTTTG+AGG + chr2.1:55702067-55702086 None:intergenic 50.0%
TGTGTCTGCGAAATCTCCAC+CGG - chr2.1:55702659-55702678 MS.gene00366:CDS 50.0%
! AGGGGGTGTGGAAGAAAGTA+GGG + chr2.1:55701743-55701762 None:intergenic 50.0%
! GAGAGTGGTGACGAGTTTAC+CGG + chr2.1:55701941-55701960 None:intergenic 50.0%
!! AAGAGTAAGGTGCTGGGGAA+TGG + chr2.1:55702044-55702063 None:intergenic 50.0%
!! ATCGGCGAAAGCATCGTCTA+CGG + chr2.1:55702322-55702341 None:intergenic 50.0%
!! TGAGGAAGAGTAAGGTGCTG+GGG + chr2.1:55702049-55702068 None:intergenic 50.0%
ACCAGGAGAGGAATTGGAGG+AGG + chr2.1:55701894-55701913 None:intergenic 55.0%
AGGGAAGGTTGAAAGGAGGG+AGG + chr2.1:55701701-55701720 None:intergenic 55.0%
AGGTTGAAAGGAGGGAGGTG+AGG + chr2.1:55701696-55701715 None:intergenic 55.0%
ATTGCGGTGGGAGAGATCAG+CGG + chr2.1:55701770-55701789 None:intergenic 55.0%
CGGGGAGTGAGTGATGTTGA+CGG + chr2.1:55702001-55702020 None:intergenic 55.0%
CTTTCTTCACCGCCAGGGTT+TGG - chr2.1:55702753-55702772 MS.gene00366:CDS 55.0%
GAATTCGAAGCCGGTGAAGG+TGG - chr2.1:55702273-55702292 MS.gene00366:CDS 55.0%
GACACTTGCGACGGAAGCTA+TGG - chr2.1:55702467-55702486 MS.gene00366:CDS 55.0%
GACGGAGCCAAAGTTGTTGG+TGG + chr2.1:55701983-55702002 None:intergenic 55.0%
GAGGTTATGGAGGTGAGGGT+CGG + chr2.1:55701833-55701852 None:intergenic 55.0%
GATACCGGAACCACCTTCAC+CGG + chr2.1:55702286-55702305 None:intergenic 55.0%
GCAGCATCCTCATAAGGAGG+AGG + chr2.1:55702696-55702715 None:intergenic 55.0%
GCTTCAATGCTCTCCGCTTC+CGG - chr2.1:55702240-55702259 MS.gene00366:CDS 55.0%
GGAGAGAGAGTGAATTGCGG+TGG + chr2.1:55701783-55701802 None:intergenic 55.0%
GGCTGTACATCAAAACCCGC+CGG + chr2.1:55702726-55702745 None:intergenic 55.0%
GGGAGAGGTTATGGAGGTGA+GGG + chr2.1:55701837-55701856 None:intergenic 55.0%
GGGTGTGGAAGAAAGTAGGG+AGG + chr2.1:55701740-55701759 None:intergenic 55.0%
GTCGGTGGTGAGATAGGTGT+TGG + chr2.1:55701815-55701834 None:intergenic 55.0%
GTTGCAACCGACACTTGCGA+CGG - chr2.1:55702458-55702477 MS.gene00366:CDS 55.0%
TCCTCCTCCAATTCCTCTCC+TGG - chr2.1:55701890-55701909 MS.gene00366:CDS 55.0%
TGACGAGTTTACCGGAGGGA+GGG + chr2.1:55701933-55701952 None:intergenic 55.0%
TTCATTCACGACGCCGGAAG+CGG + chr2.1:55702256-55702275 None:intergenic 55.0%
! AGTGGTGACGAGTTTACCGG+AGG + chr2.1:55701938-55701957 None:intergenic 55.0%
! CGGTGGTGAGATAGGTGTTG+GGG + chr2.1:55701813-55701832 None:intergenic 55.0%
! GAGGGGGTGTGGAAGAAAGT+AGG + chr2.1:55701744-55701763 None:intergenic 55.0%
! GTGGATTGAAGCGAACCGAG+AGG + chr2.1:55702429-55702448 None:intergenic 55.0%
! GTGGTGACGAGTTTACCGGA+GGG + chr2.1:55701937-55701956 None:intergenic 55.0%
! TGAGGATGCTGCTTCTTCGC+CGG - chr2.1:55702704-55702723 MS.gene00366:CDS 55.0%
!! AGCCAAAGTTGTTGGTGGCC+CGG + chr2.1:55701978-55701997 None:intergenic 55.0%
!! GGTGAGATAGGTGTTGGGGA+CGG + chr2.1:55701809-55701828 None:intergenic 55.0%
!! GTCGCAAGTGTCGGTTGCAA+CGG + chr2.1:55702459-55702478 None:intergenic 55.0%
!! TCGGCGAAAGCATCGTCTAC+GGG + chr2.1:55702321-55702340 None:intergenic 55.0%
CGGACCAGGAGAGGAATTGG+AGG + chr2.1:55701897-55701916 None:intergenic 60.0%
CTCTCTTCCAAACCACCGGC+CGG - chr2.1:55701956-55701975 MS.gene00366:CDS 60.0%
GAAGCCGGTGAAGGTGGTTC+CGG - chr2.1:55702279-55702298 MS.gene00366:CDS 60.0%
GAGAGATCAGCGGTGAGAGG+GGG + chr2.1:55701760-55701779 None:intergenic 60.0%
GGAGAGATCAGCGGTGAGAG+GGG + chr2.1:55701761-55701780 None:intergenic 60.0%
GGGAGAGATCAGCGGTGAGA+GGG + chr2.1:55701762-55701781 None:intergenic 60.0%
GGGGAGAGGTTATGGAGGTG+AGG + chr2.1:55701838-55701857 None:intergenic 60.0%
GGTCGGACCAGGAGAGGAAT+TGG + chr2.1:55701900-55701919 None:intergenic 60.0%
GTGACGAGTTTACCGGAGGG+AGG + chr2.1:55701934-55701953 None:intergenic 60.0%
GTGAGGGTCGGTGGTGAGAT+AGG + chr2.1:55701821-55701840 None:intergenic 60.0%
GTTATGGAGGTGAGGGTCGG+TGG + chr2.1:55701830-55701849 None:intergenic 60.0%
TCTCTTCCAAACCACCGGCC+GGG - chr2.1:55701957-55701976 MS.gene00366:CDS 60.0%
TGGGAGAGATCAGCGGTGAG+AGG + chr2.1:55701763-55701782 None:intergenic 60.0%
! CGGTTGCAACGGATTCGCAG+TGG + chr2.1:55702448-55702467 None:intergenic 60.0%
! GCCGGTGGTTTGGAAGAGAG+TGG + chr2.1:55701956-55701975 None:intergenic 60.0%
! GTAGCGGAGGATGTCAGCGA+GGG + chr2.1:55701866-55701885 None:intergenic 60.0%
!! AAAGTTGTTGGTGGCCCGGC+CGG + chr2.1:55701974-55701993 None:intergenic 60.0%
!! CGGCGAAAGCATCGTCTACG+GGG + chr2.1:55702320-55702339 None:intergenic 60.0%
!! GGAGGAGGAGCACTACTCTC+CGG + chr2.1:55702681-55702700 None:intergenic 60.0%
AGGATGTCAGCGAGGGCGGA+GGG + chr2.1:55701859-55701878 None:intergenic 65.0%
ATCAGCGGTGAGAGGGGGTG+TGG + chr2.1:55701755-55701774 None:intergenic 65.0%
CGAGTTTACCGGAGGGAGGG+AGG + chr2.1:55701930-55701949 None:intergenic 65.0%
CGGAGGGGGAGAGGTTATGG+AGG + chr2.1:55701843-55701862 None:intergenic 65.0%
CTCCAACACCTCCCTCCCTC+CGG - chr2.1:55701919-55701938 MS.gene00366:CDS 65.0%
GCGACGGAAGCTATGGGTGC+TGG - chr2.1:55702474-55702493 MS.gene00366:CDS 65.0%
GGCCGGGCCACCAACAACTT+TGG - chr2.1:55701973-55701992 MS.gene00366:CDS 65.0%
GTTGGAGGTCGGACCAGGAG+AGG + chr2.1:55701906-55701925 None:intergenic 65.0%
! GATGCTGCTTCTTCGCCGGC+GGG - chr2.1:55702708-55702727 MS.gene00366:CDS 65.0%
! GGATGCTGCTTCTTCGCCGG+CGG - chr2.1:55702707-55702726 MS.gene00366:CDS 65.0%
! TACCGGAGGGAGGGAGGTGT+TGG + chr2.1:55701924-55701943 None:intergenic 65.0%
!! GAGGTGTTGGAGGTCGGACC+AGG + chr2.1:55701911-55701930 None:intergenic 65.0%
!! GATAGGTGTTGGGGACGGCG+AGG + chr2.1:55701804-55701823 None:intergenic 65.0%
!! GGAGGAGCACTACTCTCCGG+TGG + chr2.1:55702678-55702697 None:intergenic 65.0%
!! GGAGGAGGATGTGGTAGCGG+AGG + chr2.1:55701879-55701898 None:intergenic 65.0%
!! GGGAGGGAGGTGTTGGAGGT+CGG + chr2.1:55701917-55701936 None:intergenic 65.0%
!! GGTAGCGGAGGATGTCAGCG+AGG + chr2.1:55701867-55701886 None:intergenic 65.0%
GAGGATGTCAGCGAGGGCGG+AGG + chr2.1:55701860-55701879 None:intergenic 70.0%
GATGTCAGCGAGGGCGGAGG+GGG + chr2.1:55701857-55701876 None:intergenic 70.0%
GCGGAGGATGTCAGCGAGGG+CGG + chr2.1:55701863-55701882 None:intergenic 70.0%
GGATGTCAGCGAGGGCGGAG+GGG + chr2.1:55701858-55701877 None:intergenic 70.0%
GGGCGGAGGGGGAGAGGTTA+TGG + chr2.1:55701846-55701865 None:intergenic 70.0%
!! CGGAGGGAGGGAGGTGTTGG+AGG + chr2.1:55701921-55701940 None:intergenic 70.0%
TGGTGGCCCGGCCGGTGGTT+TGG + chr2.1:55701966-55701985 None:intergenic 75.0%
!! GTTGTTGGTGGCCCGGCCGG+TGG + chr2.1:55701971-55701990 None:intergenic 75.0%
! CAGCGAGGGCGGAGGGGGAG+AGG + chr2.1:55701852-55701871 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 55701646 55702873 55701646 ID=MS.gene00366
chr2.1 mRNA 55701646 55702873 55701646 ID=MS.gene00366.t1;Parent=MS.gene00366
chr2.1 exon 55701737 55702873 55701737 ID=MS.gene00366.t1.exon1;Parent=MS.gene00366.t1
chr2.1 CDS 55701737 55702873 55701737 ID=cds.MS.gene00366.t1;Parent=MS.gene00366.t1
chr2.1 exon 55701646 55701657 55701646 ID=MS.gene00366.t1.exon2;Parent=MS.gene00366.t1
chr2.1 CDS 55701646 55701657 55701646 ID=cds.MS.gene00366.t1;Parent=MS.gene00366.t1
Gene Sequence

>MS.gene00366

ATGCTATATTTCCTAATCCTATCCCCAACACCATCTTTCTCTTTCAACCTCACCTCCCTCCTTTCAACCTTCCCTGAATTATCAACATTCACCTCCCTACTTTCTTCCACACCCCCTCTCACCGCTGATCTCTCCCACCGCAATTCACTCTCTCTCCTCGCCGTCCCCAACACCTATCTCACCACCGACCCTCACCTCCATAACCTCTCCCCCTCCGCCCTCGCTGACATCCTCCGCTACCACATCCTCCTCCAATTCCTCTCCTGGTCCGACCTCCAACACCTCCCTCCCTCCGGTAAACTCGTCACCACTCTCTTCCAAACCACCGGCCGGGCCACCAACAACTTTGGCTCCGTCAACATCACTCACTCCCCGAACACCAACACCATTACAATCCATTCCCCAGCACCTTACTCTTCCTCAAACGCCACCGTTCTTTCACAGCTCAAAATGTTACCCTACAATCTCACTATCTTCACCGTTGATTCTCTTTTAATCCCTTACGGTTTCGATCTCATGGCATCGGAAACTCGTCCAAGCATTCTTCTCAACATCACCAAAACCCTAATCGATGCTCACAATTTCAACGTCGCTGCTTCAATGCTCTCCGCTTCCGGCGTCGTGAATGAATTCGAAGCCGGTGAAGGTGGTTCCGGTATCACCTTGTTTATCCCCGTAGACGATGCTTTCGCCGATCTACCTCCTTCCGTTTCGCTTCAATCACTTCCCGCCGATCAAAAAGCAGTTGTTCTTAAAGCGCATGTTCTTCGTGCTTATTATCCTCTCGGTTCGCTTCAATCCACTGCGAATCCGTTGCAACCGACACTTGCGACGGAAGCTATGGGTGCTGGAAGTTTCACGTTGAATATTTCAACTTTTAATGGCTCTGTTGCGATTAACACTGGCATTGTTCAGGGGATAATTACTCAGACAGTGTTTGATCAGAATCCTATTGCGATTTTCGGTGTTTCGAAGGTTTTGTTGCCAAGAGAGATATTTGGGAAGAATCCAATTGTGTCTGCGAAATCTCCACCGGAGAGTAGTGCTCCTCCTCCTTATGAGGATGCTGCTTCTTCGCCGGCGGGTTTTGATGTACAGCCGTCACATCTTTCTTCACCGCCAGGGTTTGGTGAAGATGTTAGCTCAGATGTTGCAAGTGTTTATGGTTCTGAGTTATTTGTTTTTCTACTTTGTTGTATAAATTTGTATTTAATAGTATAAAGAGTAA
Protein sequence

>MS.gene00366.t1

MLYFLILSPTPSFSFNLTSLLSTFPELSTFTSLLSSTPPLTADLSHRNSLSLLAVPNTYLTTDPHLHNLSPSALADILRYHILLQFLSWSDLQHLPPSGKLVTTLFQTTGRATNNFGSVNITHSPNTNTITIHSPAPYSSSNATVLSQLKMLPYNLTIFTVDSLLIPYGFDLMASETRPSILLNITKTLIDAHNFNVAASMLSASGVVNEFEAGEGGSGITLFIPVDDAFADLPPSVSLQSLPADQKAVVLKAHVLRAYYPLGSLQSTANPLQPTLATEAMGAGSFTLNISTFNGSVAINTGIVQGIITQTVFDQNPIAIFGVSKVLLPREIFGKNPIVSAKSPPESSAPPPYEDAASSPAGFDVQPSHLSSPPGFGEDYKE