AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004517.t1 MTR_2g021130 100.000 257 0 0 1 257 1 257 0.0 522
MS.gene004517.t1 MTR_1g071430 51.709 234 108 3 28 257 12 244 2.93e-78 236
MS.gene004517.t1 MTR_7g095640 53.586 237 105 3 24 256 2 237 7.02e-78 235
MS.gene004517.t1 MTR_1g110390 45.299 234 124 2 24 254 7 239 1.24e-65 204
MS.gene004517.t1 MTR_7g095530 44.206 233 121 4 28 254 6 235 1.76e-52 171
MS.gene004517.t1 MTR_7g095510 46.491 228 117 4 28 251 6 232 2.91e-52 170
MS.gene004517.t1 MTR_7g095600 43.750 224 120 5 28 246 6 228 5.88e-51 167
MS.gene004517.t1 MTR_7g095550 47.368 76 40 0 137 212 20 95 1.29e-16 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004517.t1 AT4G15470 65.339 251 78 2 16 257 6 256 2.60e-111 321
MS.gene004517.t1 AT1G03070 52.500 240 109 3 21 256 9 247 8.29e-84 251
MS.gene004517.t1 AT1G03070 52.500 240 109 3 21 256 9 247 8.29e-84 251
MS.gene004517.t1 AT1G03070 52.500 240 109 3 21 256 9 247 8.29e-84 251
MS.gene004517.t1 AT4G02690 51.883 239 108 4 21 254 9 245 6.54e-77 233
MS.gene004517.t1 AT3G63310 51.261 238 103 5 28 257 7 239 4.68e-74 226
MS.gene004517.t1 AT4G14730 45.923 233 120 4 25 252 2 233 5.84e-65 202
MS.gene004517.t1 AT4G02690 44.068 236 91 6 21 253 9 206 1.80e-51 167

Find 72 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAACTGCTTATGCTTTC+TGG -0.049762 2.4:+66087079 MS.gene004517:CDS
GCTAAAATCCTTGCCCTTCT+TGG 0.234291 2.4:-66087106 None:intergenic
TCTAACTGCTTATGCTTTCT+GGG 0.272407 2.4:+66087080 MS.gene004517:CDS
GTGCAGATGTTCTTCCCTCT+TGG 0.303829 2.4:+66087905 MS.gene004517:intron
ATTGAAAGCGTACAGATAAA+TGG 0.313565 2.4:-66086544 None:intergenic
CTCCTCTTAATCTTCTCCTT+AGG 0.318409 2.4:+66086485 MS.gene004517:CDS
GAAAAGAGATGAAGCTGAAT+TGG 0.341580 2.4:+66086318 MS.gene004517:CDS
CAATGTATTCATCATAAGTA+TGG 0.358120 2.4:-66088015 None:intergenic
CCATAGATAGCATGTGATAC+TGG 0.365656 2.4:-66087929 None:intergenic
GCTGAATTGGACATCGAATC+TGG 0.367286 2.4:+66086331 MS.gene004517:CDS
TCCTCTTAATCTTCTCCTTA+GGG 0.369097 2.4:+66086486 MS.gene004517:CDS
CGGTGAAAATCAACTCCGTT+GGG 0.369557 2.4:+66086381 MS.gene004517:CDS
TGCAGATGTTCTTCCCTCTT+GGG 0.369643 2.4:+66087906 MS.gene004517:intron
CCAGTATCACATGCTATCTA+TGG 0.386108 2.4:+66087929 MS.gene004517:CDS
CCTCATAATTATATCTTGCT+TGG 0.408556 2.4:+66086694 MS.gene004517:CDS
ACCTGTTCCTTTCCATCTTG+AGG 0.417636 2.4:+66088071 MS.gene004517:CDS
TCCTCAAGATGGAAAGGAAC+AGG 0.423761 2.4:-66088072 None:intergenic
AACTCCAACGGTGAAGCTAA+TGG 0.425315 2.4:-66086731 None:intergenic
ATACTTATGATGAATACATT+GGG 0.443963 2.4:+66088017 MS.gene004517:CDS
CGGAGACGATGGTGGTTAAG+AGG 0.453233 2.4:-66086444 None:intergenic
TGCTTTCTGGGCTTCCAAGA+AGG 0.469986 2.4:+66087092 MS.gene004517:CDS
CCCCTAAGGAGAAGATTAAG+AGG 0.472124 2.4:-66086487 None:intergenic
CGAGAGAGAGACCTGGGTAG+AGG 0.472666 2.4:-66086360 None:intergenic
CTGGGCTTCCAAGAAGGGCA+AGG 0.482805 2.4:+66087098 MS.gene004517:CDS
ATTAAGAGGAGTATAGAAAA+CGG 0.483866 2.4:-66086473 None:intergenic
TTTCCATCTTGAGGATTCTG+AGG 0.486855 2.4:+66088080 MS.gene004517:CDS
TGTACTCACCGGCATGATGC+AGG 0.493957 2.4:+66087167 MS.gene004517:CDS
CTCTTTACTCTTGTACTCAC+CGG 0.497829 2.4:+66087156 MS.gene004517:CDS
TCGGTGAAAATCAACTCCGT+TGG 0.502314 2.4:+66086380 MS.gene004517:CDS
AGACGAGGAACAAGAGAAGA+GGG 0.503884 2.4:-66086516 None:intergenic
CCAAGCAAGATATAATTATG+AGG 0.505817 2.4:-66086694 None:intergenic
TTCCATCTTGAGGATTCTGA+GGG 0.509793 2.4:+66088081 MS.gene004517:CDS
GAAGAGGGGAATTGCCCCTA+AGG 0.512979 2.4:-66086501 None:intergenic
CATACTTATGATGAATACAT+TGG 0.514094 2.4:+66088016 MS.gene004517:CDS
GCTTTCTGGGCTTCCAAGAA+GGG 0.517151 2.4:+66087093 MS.gene004517:CDS
GGTTTCATTCGCAAAGTCTA+CGG 0.522355 2.4:+66086403 MS.gene004517:CDS
CTCTACCCAGGTCTCTCTCT+CGG 0.533320 2.4:+66086361 MS.gene004517:CDS
CATCTTGAGGATTCTGAGGG+AGG 0.543704 2.4:+66088084 MS.gene004517:CDS
GAGAGAGAGACCTGGGTAGA+GGG 0.551968 2.4:-66086359 None:intergenic
CATGCTATCTATGGTGGAGT+TGG 0.558076 2.4:+66087938 MS.gene004517:CDS
TTGTCCATTAGCTTCACCGT+TGG 0.565046 2.4:+66086727 MS.gene004517:CDS
AAGAGGCAGGTAAACAAAAG+AGG 0.565428 2.4:-66087138 None:intergenic
CTCCCTCAGAATCCTCAAGA+TGG 0.569106 2.4:-66088083 None:intergenic
AGTATAGAAAACGGTTATGA+CGG 0.569627 2.4:-66086464 None:intergenic
CCTCGTTTATCTTGTTGATG+CGG 0.569798 2.4:-66086283 None:intergenic
GAGTACAAGAGTAAAGAGGC+AGG 0.577257 2.4:-66087151 None:intergenic
TCAGAATCCTCAAGATGGAA+AGG 0.577543 2.4:-66088078 None:intergenic
AACGGTTATGACGGAGACGA+TGG 0.586691 2.4:-66086455 None:intergenic
CGTTTATCTTGTTGATGCGG+TGG 0.590169 2.4:-66086280 None:intergenic
GCAACCAACCTGCATCATGC+CGG 0.592343 2.4:-66087175 None:intergenic
CCTCTTAATCTTCTCCTTAG+GGG 0.595131 2.4:+66086487 MS.gene004517:CDS
CGGTGAGTACAAGAGTAAAG+AGG 0.595420 2.4:-66087155 None:intergenic
AAGACGAGGAACAAGAGAAG+AGG 0.601856 2.4:-66086517 None:intergenic
GTATCACATGCTATCTATGG+TGG 0.627746 2.4:+66087932 MS.gene004517:CDS
TTTCACCGAGAGAGAGACCT+GGG 0.628985 2.4:-66086366 None:intergenic
GCATGTGATACTGGCCCAAG+AGG 0.640221 2.4:-66087920 None:intergenic
GGTTATGACGGAGACGATGG+TGG 0.641554 2.4:-66086452 None:intergenic
AACGGTGAAGCTAATGGACA+AGG 0.644202 2.4:-66086725 None:intergenic
TCTGGAGAAACCCTCTACCC+AGG 0.652055 2.4:+66086349 MS.gene004517:CDS
GTTACTTGTGCAAATACTGA+TGG 0.653520 2.4:+66086751 MS.gene004517:CDS
CATGTGATACTGGCCCAAGA+GGG 0.654330 2.4:-66087919 None:intergenic
GGTGAAAATCAACTCCGTTG+GGG 0.660285 2.4:+66086382 MS.gene004517:CDS
GTCGAGATAAAGAGTGACAG+AGG 0.674076 2.4:-66088041 None:intergenic
TACTTATGATGAATACATTG+GGG 0.675391 2.4:+66088018 MS.gene004517:CDS
GACGAGGAACAAGAGAAGAG+GGG 0.683500 2.4:-66086515 None:intergenic
AGGTAAACAAAAGAGGACCA+AGG 0.683783 2.4:-66087131 None:intergenic
ATTTGCACAAGTAACTCCAA+CGG 0.706602 2.4:-66086743 None:intergenic
TTAAGAGGATCTGAGCGGAG+AGG 0.713415 2.4:-66086429 None:intergenic
CCGCATCAACAAGATAAACG+AGG 0.719389 2.4:+66086283 MS.gene004517:CDS
AGATAAATGGAAGAAAGACG+AGG 0.735810 2.4:-66086531 None:intergenic
CTTTGCGAATGAAACCCCAA+CGG 0.748117 2.4:-66086396 None:intergenic
GGTGGTTAAGAGGATCTGAG+CGG 0.764189 2.4:-66086434 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATTAAAAAAAAATCAA+AGG - chr2.4:66086577-66086596 None:intergenic 10.0%
!!! AATTCAATATTTTGAATTAA+AGG + chr2.4:66087611-66087630 MS.gene004517:intron 10.0%
!!! ATTCAATATTTTGAATTAAA+GGG + chr2.4:66087612-66087631 MS.gene004517:intron 10.0%
!! CTTTCTATTATTTATCTATT+TGG + chr2.4:66086805-66086824 MS.gene004517:intron 15.0%
!! GAATGTAAGTAAATTTATTT+AGG + chr2.4:66086865-66086884 MS.gene004517:intron 15.0%
!! TAAACTATTATTACTTTGAT+TGG + chr2.4:66086980-66086999 MS.gene004517:intron 15.0%
!!! AATTGTTTTATGAATTGATT+AGG + chr2.4:66086590-66086609 MS.gene004517:intron 15.0%
!!! AATTTAGAAGAATCAATTTT+TGG + chr2.4:66086934-66086953 MS.gene004517:intron 15.0%
!!! AGAGTTTTGAATTTTAAAAT+TGG + chr2.4:66087711-66087730 MS.gene004517:intron 15.0%
!!! ATTGTTTTATGAATTGATTA+GGG + chr2.4:66086591-66086610 MS.gene004517:intron 15.0%
!! AAAAAAAAATCAAAGGATGA+AGG - chr2.4:66086570-66086589 None:intergenic 20.0%
!! AAGTAATAATAGTTTATGCT+CGG - chr2.4:66086976-66086995 None:intergenic 20.0%
!! AATAGAAAGAGAATAACAAA+TGG - chr2.4:66086794-66086813 None:intergenic 20.0%
!! ATACTTATGATGAATACATT+GGG + chr2.4:66088017-66088036 MS.gene004517:CDS 20.0%
!! ATGATACAAAAATAAATGAC+AGG - chr2.4:66087210-66087229 None:intergenic 20.0%
!! GATTAAGAATGGAATTATTA+GGG + chr2.4:66087380-66087399 MS.gene004517:intron 20.0%
!!! TATTTATCTATTTGGTTTTG+CGG + chr2.4:66086813-66086832 MS.gene004517:intron 20.0%
! CAATGTATTCATCATAAGTA+TGG - chr2.4:66088018-66088037 None:intergenic 25.0%
! CATACTTATGATGAATACAT+TGG + chr2.4:66088016-66088035 MS.gene004517:CDS 25.0%
! GGATTAAGAATGGAATTATT+AGG + chr2.4:66087379-66087398 MS.gene004517:intron 25.0%
! TACTTATGATGAATACATTG+GGG + chr2.4:66088018-66088037 MS.gene004517:CDS 25.0%
!! ATTAAGAGGAGTATAGAAAA+CGG - chr2.4:66086476-66086495 None:intergenic 25.0%
!! TTCTACCATATTTTCTACAA+TGG - chr2.4:66087422-66087441 None:intergenic 25.0%
!! TTGTATCATTGATTGATAAC+TGG + chr2.4:66087221-66087240 MS.gene004517:intron 25.0%
!!! GTTAAACCTTTTTTCCTTAT+AGG - chr2.4:66087806-66087825 None:intergenic 25.0%
ACACGGTGAATGATAATATT+GGG + chr2.4:66087322-66087341 MS.gene004517:intron 30.0%
ACATTGGATAGGATTAAGAA+TGG + chr2.4:66087369-66087388 MS.gene004517:intron 30.0%
AGTATAGAAAACGGTTATGA+CGG - chr2.4:66086467-66086486 None:intergenic 30.0%
ATAATATTGGGATGGATAAG+TGG + chr2.4:66087334-66087353 MS.gene004517:intron 30.0%
CCAAGCAAGATATAATTATG+AGG - chr2.4:66086697-66086716 None:intergenic 30.0%
CCTCATAATTATATCTTGCT+TGG + chr2.4:66086694-66086713 MS.gene004517:CDS 30.0%
GAAAATATGGTAGAAATCAC+TGG + chr2.4:66087427-66087446 MS.gene004517:intron 30.0%
TACACGGTGAATGATAATAT+TGG + chr2.4:66087321-66087340 MS.gene004517:intron 30.0%
TTATAGTGAATCACACCTAA+TGG - chr2.4:66087747-66087766 None:intergenic 30.0%
TTTCAATATGAACGAATGTG+AGG + chr2.4:66087278-66087297 MS.gene004517:intron 30.0%
! ATTGAAAGCGTACAGATAAA+TGG - chr2.4:66086547-66086566 None:intergenic 30.0%
! ATTGATTGATAACTGGTTGT+TGG + chr2.4:66087228-66087247 MS.gene004517:intron 30.0%
AAATGACCTAGTCTAAGTCT+AGG + chr2.4:66087522-66087541 MS.gene004517:intron 35.0%
AGATAAATGGAAGAAAGACG+AGG - chr2.4:66086534-66086553 None:intergenic 35.0%
ATTTGCACAAGTAACTCCAA+CGG - chr2.4:66086746-66086765 None:intergenic 35.0%
GAAAAGAGATGAAGCTGAAT+TGG + chr2.4:66086318-66086337 MS.gene004517:CDS 35.0%
GATAATGAAAAGCTGTCTCT+AGG - chr2.4:66087661-66087680 None:intergenic 35.0%
GCATCACCTATAAGGAAAAA+AGG + chr2.4:66087797-66087816 MS.gene004517:intron 35.0%
GGTGAATGATAATATTGGGA+TGG + chr2.4:66087326-66087345 MS.gene004517:intron 35.0%
GTTACTTGTGCAAATACTGA+TGG + chr2.4:66086751-66086770 MS.gene004517:CDS 35.0%
TAAAAGGAGGATAGATGAGA+TGG + chr2.4:66087583-66087602 MS.gene004517:intron 35.0%
TATCATATGCTTTGACACCA+CGG + chr2.4:66087677-66087696 MS.gene004517:intron 35.0%
TATGTCTGTTGTTGTAATGC+AGG + chr2.4:66087009-66087028 MS.gene004517:intron 35.0%
TCCTCTTAATCTTCTCCTTA+GGG + chr2.4:66086486-66086505 MS.gene004517:CDS 35.0%
TCGCACCATTGTAGAAAATA+TGG + chr2.4:66087414-66087433 MS.gene004517:intron 35.0%
TCTAACTGCTTATGCTTTCT+GGG + chr2.4:66087080-66087099 MS.gene004517:CDS 35.0%
TGAATCACACCTAATGGAAA+AGG - chr2.4:66087741-66087760 None:intergenic 35.0%
TTGTGCAAATACTGATGGTA+GGG + chr2.4:66086756-66086775 MS.gene004517:intron 35.0%
! AATTATGAGGATGCTTTTGC+TGG - chr2.4:66086684-66086703 None:intergenic 35.0%
! GATTAACAGAAGCACGTAAA+AGG + chr2.4:66087567-66087586 MS.gene004517:intron 35.0%
! GCTCAAGACTATATTTTCTC+TGG + chr2.4:66087883-66087902 MS.gene004517:intron 35.0%
!! GACTTAGACTAGGTCATTTT+TGG - chr2.4:66087521-66087540 None:intergenic 35.0%
!! TTTATGCTCGGTTCGAATTT+TGG - chr2.4:66086964-66086983 None:intergenic 35.0%
!!! TAGTTTTGATTCCGTTGTTG+AGG + chr2.4:66086656-66086675 MS.gene004517:intron 35.0%
!!! TTGATTAGGGTTTTATTGCG+TGG + chr2.4:66086604-66086623 MS.gene004517:intron 35.0%
AAAAATTGTGCTTGAGGCGT+TGG + chr2.4:66087032-66087051 MS.gene004517:CDS 40.0%
AAGAGGCAGGTAAACAAAAG+AGG - chr2.4:66087141-66087160 None:intergenic 40.0%
AGGAGTTTCGGAATCAACAA+CGG - chr2.4:66087641-66087660 None:intergenic 40.0%
AGGTAAACAAAAGAGGACCA+AGG - chr2.4:66087134-66087153 None:intergenic 40.0%
ATATGGTAGAAATCACTGGC+TGG + chr2.4:66087431-66087450 MS.gene004517:intron 40.0%
CATGTGTTGCATCACCTATA+AGG + chr2.4:66087789-66087808 MS.gene004517:intron 40.0%
CCAGTATCACATGCTATCTA+TGG + chr2.4:66087929-66087948 MS.gene004517:CDS 40.0%
CCATAGATAGCATGTGATAC+TGG - chr2.4:66087932-66087951 None:intergenic 40.0%
CCTCGTTTATCTTGTTGATG+CGG - chr2.4:66086286-66086305 None:intergenic 40.0%
CCTCTTAATCTTCTCCTTAG+GGG + chr2.4:66086487-66086506 MS.gene004517:CDS 40.0%
CTCCTCTTAATCTTCTCCTT+AGG + chr2.4:66086485-66086504 MS.gene004517:CDS 40.0%
CTCTAACTGCTTATGCTTTC+TGG + chr2.4:66087079-66087098 MS.gene004517:CDS 40.0%
CTCTTTACTCTTGTACTCAC+CGG + chr2.4:66087156-66087175 MS.gene004517:CDS 40.0%
CTTGTGCAAATACTGATGGT+AGG + chr2.4:66086755-66086774 MS.gene004517:intron 40.0%
GGTTTCATTCGCAAAGTCTA+CGG + chr2.4:66086403-66086422 MS.gene004517:CDS 40.0%
GTATCACATGCTATCTATGG+TGG + chr2.4:66087932-66087951 MS.gene004517:CDS 40.0%
TCAGAATCCTCAAGATGGAA+AGG - chr2.4:66088081-66088100 None:intergenic 40.0%
TGCAGGAAAAATTGTGCTTG+AGG + chr2.4:66087026-66087045 MS.gene004517:intron 40.0%
TTCCATCTTGAGGATTCTGA+GGG + chr2.4:66088081-66088100 MS.gene004517:CDS 40.0%
TTTCCATCTTGAGGATTCTG+AGG + chr2.4:66088080-66088099 MS.gene004517:CDS 40.0%
! AAAGCTGTCTCTAGGAGTTT+CGG - chr2.4:66087653-66087672 None:intergenic 40.0%
! AACTCTTGTGTTTGAAACCG+TGG - chr2.4:66087697-66087716 None:intergenic 40.0%
! GGCGACACAAAAATTGATTC+TGG + chr2.4:66086834-66086853 MS.gene004517:intron 40.0%
! TAACAGAAGCACGTAAAAGG+AGG + chr2.4:66087570-66087589 MS.gene004517:intron 40.0%
! TTTTGCTGGTACCTCAACAA+CGG - chr2.4:66086670-66086689 None:intergenic 40.0%
!! AACTGGTTGTTGGTGTTTTC+TGG + chr2.4:66087238-66087257 MS.gene004517:intron 40.0%
!! ATTGGCTCTCCTTTTCCATT+AGG + chr2.4:66087729-66087748 MS.gene004517:intron 40.0%
!! CTCTGTGTGTTGTTTCTACA+CGG + chr2.4:66087305-66087324 MS.gene004517:intron 40.0%
AACTCCAACGGTGAAGCTAA+TGG - chr2.4:66086734-66086753 None:intergenic 45.0%
AAGACGAGGAACAAGAGAAG+AGG - chr2.4:66086520-66086539 None:intergenic 45.0%
ACCTGTTCCTTTCCATCTTG+AGG + chr2.4:66088071-66088090 MS.gene004517:CDS 45.0%
AGACCACCTAGACTTAGACT+AGG - chr2.4:66087531-66087550 None:intergenic 45.0%
AGACGAGGAACAAGAGAAGA+GGG - chr2.4:66086519-66086538 None:intergenic 45.0%
AGTCTAAGTCTAGGTGGTCT+AGG + chr2.4:66087531-66087550 MS.gene004517:intron 45.0%
CACGCAGGATAACATTGGAT+AGG + chr2.4:66087358-66087377 MS.gene004517:intron 45.0%
CCCCTAAGGAGAAGATTAAG+AGG - chr2.4:66086490-66086509 None:intergenic 45.0%
CCGCATCAACAAGATAAACG+AGG + chr2.4:66086283-66086302 MS.gene004517:CDS 45.0%
CGGTGAAAATCAACTCCGTT+GGG + chr2.4:66086381-66086400 MS.gene004517:CDS 45.0%
CGGTGAGTACAAGAGTAAAG+AGG - chr2.4:66087158-66087177 None:intergenic 45.0%
CGTGTAGAAACAACACACAG+AGG - chr2.4:66087307-66087326 None:intergenic 45.0%
CGTTTATCTTGTTGATGCGG+TGG - chr2.4:66086283-66086302 None:intergenic 45.0%
CTTTGCGAATGAAACCCCAA+CGG - chr2.4:66086399-66086418 None:intergenic 45.0%
GAGTACAAGAGTAAAGAGGC+AGG - chr2.4:66087154-66087173 None:intergenic 45.0%
GCTAAAATCCTTGCCCTTCT+TGG - chr2.4:66087109-66087128 None:intergenic 45.0%
GCTGAATTGGACATCGAATC+TGG + chr2.4:66086331-66086350 MS.gene004517:CDS 45.0%
GGTGAAAATCAACTCCGTTG+GGG + chr2.4:66086382-66086401 MS.gene004517:CDS 45.0%
GTCGAGATAAAGAGTGACAG+AGG - chr2.4:66088044-66088063 None:intergenic 45.0%
TCCTCAAGATGGAAAGGAAC+AGG - chr2.4:66088075-66088094 None:intergenic 45.0%
TCGGTGAAAATCAACTCCGT+TGG + chr2.4:66086380-66086399 MS.gene004517:CDS 45.0%
TGACCTAGTCTAAGTCTAGG+TGG + chr2.4:66087525-66087544 MS.gene004517:intron 45.0%
TGCAGATGTTCTTCCCTCTT+GGG + chr2.4:66087906-66087925 MS.gene004517:intron 45.0%
! AACGGTGAAGCTAATGGACA+AGG - chr2.4:66086728-66086747 None:intergenic 45.0%
! CATGCTATCTATGGTGGAGT+TGG + chr2.4:66087938-66087957 MS.gene004517:CDS 45.0%
! TTGTCCATTAGCTTCACCGT+TGG + chr2.4:66086727-66086746 MS.gene004517:CDS 45.0%
!!! GGCAAGGATTTTAGCTACCT+TGG + chr2.4:66087114-66087133 MS.gene004517:CDS 45.0%
CATGTGATACTGGCCCAAGA+GGG - chr2.4:66087922-66087941 None:intergenic 50.0%
CTAGGTGGTCTAGGAATGTG+TGG + chr2.4:66087540-66087559 MS.gene004517:intron 50.0%
CTCCCTCAGAATCCTCAAGA+TGG - chr2.4:66088086-66088105 None:intergenic 50.0%
GACGAGGAACAAGAGAAGAG+GGG - chr2.4:66086518-66086537 None:intergenic 50.0%
GCTTTCTGGGCTTCCAAGAA+GGG + chr2.4:66087093-66087112 MS.gene004517:CDS 50.0%
GGTGGTTAAGAGGATCTGAG+CGG - chr2.4:66086437-66086456 None:intergenic 50.0%
GTGCAGATGTTCTTCCCTCT+TGG + chr2.4:66087905-66087924 MS.gene004517:intron 50.0%
GTGGTCACGCAGGATAACAT+TGG + chr2.4:66087353-66087372 MS.gene004517:intron 50.0%
TGCTTTCTGGGCTTCCAAGA+AGG + chr2.4:66087092-66087111 MS.gene004517:CDS 50.0%
TTAAGAGGATCTGAGCGGAG+AGG - chr2.4:66086432-66086451 None:intergenic 50.0%
TTTCACCGAGAGAGAGACCT+GGG - chr2.4:66086369-66086388 None:intergenic 50.0%
! AACGGTTATGACGGAGACGA+TGG - chr2.4:66086458-66086477 None:intergenic 50.0%
! CATCTTGAGGATTCTGAGGG+AGG + chr2.4:66088084-66088103 MS.gene004517:CDS 50.0%
! TTTTCACCGAGAGAGAGACC+TGG - chr2.4:66086370-66086389 None:intergenic 50.0%
!!! GGCGTTGGTTTTGACATCTG+CGG + chr2.4:66087047-66087066 MS.gene004517:CDS 50.0%
!!! GTTGGTTTTGACATCTGCGG+TGG + chr2.4:66087050-66087069 MS.gene004517:CDS 50.0%
CTCTACCCAGGTCTCTCTCT+CGG + chr2.4:66086361-66086380 MS.gene004517:CDS 55.0%
GAAGAGGGGAATTGCCCCTA+AGG - chr2.4:66086504-66086523 None:intergenic 55.0%
GAGAGAGAGACCTGGGTAGA+GGG - chr2.4:66086362-66086381 None:intergenic 55.0%
GCAACCAACCTGCATCATGC+CGG - chr2.4:66087178-66087197 None:intergenic 55.0%
GCATGTGATACTGGCCCAAG+AGG - chr2.4:66087923-66087942 None:intergenic 55.0%
GGATGGATAAGTGGTCACGC+AGG + chr2.4:66087343-66087362 MS.gene004517:intron 55.0%
TCTGGAGAAACCCTCTACCC+AGG + chr2.4:66086349-66086368 MS.gene004517:CDS 55.0%
TGTACTCACCGGCATGATGC+AGG + chr2.4:66087167-66087186 MS.gene004517:CDS 55.0%
! CGGAGACGATGGTGGTTAAG+AGG - chr2.4:66086447-66086466 None:intergenic 55.0%
! GGTTATGACGGAGACGATGG+TGG - chr2.4:66086455-66086474 None:intergenic 55.0%
CGAGAGAGAGACCTGGGTAG+AGG - chr2.4:66086363-66086382 None:intergenic 60.0%
CTCACCGGCATGATGCAGGT+TGG + chr2.4:66087171-66087190 MS.gene004517:intron 60.0%
CTGGGCTTCCAAGAAGGGCA+AGG + chr2.4:66087098-66087117 MS.gene004517:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 66086259 66088117 66086259 ID=MS.gene004517
chr2.4 mRNA 66086259 66088117 66086259 ID=MS.gene004517.t1;Parent=MS.gene004517
chr2.4 exon 66086259 66086553 66086259 ID=MS.gene004517.t1.exon1;Parent=MS.gene004517.t1
chr2.4 CDS 66086259 66086553 66086259 ID=cds.MS.gene004517.t1;Parent=MS.gene004517.t1
chr2.4 exon 66086659 66086772 66086659 ID=MS.gene004517.t1.exon2;Parent=MS.gene004517.t1
chr2.4 CDS 66086659 66086772 66086659 ID=cds.MS.gene004517.t1;Parent=MS.gene004517.t1
chr2.4 exon 66087031 66087188 66087031 ID=MS.gene004517.t1.exon3;Parent=MS.gene004517.t1
chr2.4 CDS 66087031 66087188 66087031 ID=cds.MS.gene004517.t1;Parent=MS.gene004517.t1
chr2.4 exon 66087911 66088117 66087911 ID=MS.gene004517.t1.exon4;Parent=MS.gene004517.t1
chr2.4 CDS 66087911 66088117 66087911 ID=cds.MS.gene004517.t1;Parent=MS.gene004517.t1
Gene Sequence

>MS.gene004517

ATGATGATGTTCGATCAGCAACCACCGCATCAACAAGATAAACGAGGCTACACAAGCGTGAAAAGAGATGAAGCTGAATTGGACATCGAATCTGGAGAAACCCTCTACCCAGGTCTCTCTCTCGGTGAAAATCAACTCCGTTGGGGTTTCATTCGCAAAGTCTACGGAATCCTCTCCGCTCAGATCCTCTTAACCACCATCGTCTCCGTCATAACCGTTTTCTATACTCCTCTTAATCTTCTCCTTAGGGGCAATTCCCCTCTTCTCTTGTTCCTCGTCTTTCTTCCATTTATCTGTACGCTTTCAATCCTTCATCCTTTGATTTTTTTTTAATTGTTTTATGAATTGATTAGGGTTTTATTGCGTGGATCTCATTTTGATTTTGAAATTTTTGATGTAGTTTTGATTCCGTTGTTGAGGTACCAGCAAAAGCATCCTCATAATTATATCTTGCTTGGACTTTTTACCTTGTCCATTAGCTTCACCGTTGGAGTTACTTGTGCAAATACTGATGGTAGGGTTTCGTTCTCATCCATTTGTTATTCTCTTTCTATTATTTATCTATTTGGTTTTGCGGCGACACAAAAATTGATTCTGGTTAAATTTGAATGTAAGTAAATTTATTTAGGTTTATCTAAGTAAATTTGAATGTAAGTAAATCTGATCAAAAGCTACAATTTAGAAGAATCAATTTTTGGTGCTCCAAAATTCGAACCGAGCATAAACTATTATTACTTTGATTGGTTGTAATATGTCTGTTGTTGTAATGCAGGAAAAATTGTGCTTGAGGCGTTGGTTTTGACATCTGCGGTGGTTTCTTCTCTAACTGCTTATGCTTTCTGGGCTTCCAAGAAGGGCAAGGATTTTAGCTACCTTGGTCCTCTTTTGTTTACCTGCCTCTTTACTCTTGTACTCACCGGCATGATGCAGGTTGGTTGCATTACATTTCCTGTCATTTATTTTTGTATCATTGATTGATAACTGGTTGTTGGTGTTTTCTGGCTTTAGTTTTTTATACGTTTCAATATGAACGAATGTGAGGTTTCCTCTGTGTGTTGTTTCTACACGGTGAATGATAATATTGGGATGGATAAGTGGTCACGCAGGATAACATTGGATAGGATTAAGAATGGAATTATTAGGGAAAAAAATGAGTCGCACCATTGTAGAAAATATGGTAGAAATCACTGGCTGGAATCAGTGACATTAGAAGATATGTGATCTGATCAGAAGTAAGTTTGAATCTTTATGATGTGAAACCAAAAATGACCTAGTCTAAGTCTAGGTGGTCTAGGAATGTGTGGTGAAGATTAACAGAAGCACGTAAAAGGAGGATAGATGAGATGGAATTCAATTCAATATTTTGAATTAAAGGGAGACCGTTGTTGATTCCGAAACTCCTAGAGACAGCTTTTCATTATCATATGCTTTGACACCACGGTTTCAAACACAAGAGTTTTGAATTTTAAAATTGGCTCTCCTTTTCCATTAGGTGTGATTCACTATAAGATATTATGAAATTGTTGTATTCATGTGTTGCATCACCTATAAGGAAAAAAGGTTTAACTATAGATGCACACATATGAAATTGTTGTACATAGTTTATGCTTGCATCTTTTCTCTTGCTCAAGACTATATTTTCTCTGGTGCAGATGTTCTTCCCTCTTGGGCCAGTATCACATGCTATCTATGGTGGAGTTGGTGCTATGATTTTCTCTGCTTATATTGTCTATGACACTGACAATCTGATCAAGCGCCATACTTATGATGAATACATTGGGGCCTCTGTCACTCTTTATCTCGACATTCTGAACCTGTTCCTTTCCATCTTGAGGATTCTGAGGGAGGCAAACAATTAA
Protein sequence

>MS.gene004517.t1

MMMFDQQPPHQQDKRGYTSVKRDEAELDIESGETLYPGLSLGENQLRWGFIRKVYGILSAQILLTTIVSVITVFYTPLNLLLRGNSPLLLFLVFLPFIFLIPLLRYQQKHPHNYILLGLFTLSISFTVGVTCANTDGKIVLEALVLTSAVVSSLTAYAFWASKKGKDFSYLGPLLFTCLFTLVLTGMMQMFFPLGPVSHAIYGGVGAMIFSAYIVYDTDNLIKRHTYDEYIGASVTLYLDILNLFLSILRILREANN