AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00462

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00462.t1 MTR_1g018840 97.489 438 11 0 1 438 6 443 0.0 893
MS.gene00462.t1 MTR_1g018840 97.489 438 11 0 1 438 6 443 0.0 891
MS.gene00462.t1 MTR_3g102220 72.811 434 115 2 7 438 11 443 0.0 645
MS.gene00462.t1 MTR_1g107550 68.269 416 125 3 22 436 26 435 0.0 575
MS.gene00462.t1 MTR_4g094918 64.543 361 114 1 3 363 2 348 2.29e-177 500
MS.gene00462.t1 MTR_3g112430 51.020 392 181 6 49 438 35 417 4.57e-132 388
MS.gene00462.t1 MTR_3g116080 52.339 342 151 4 16 357 19 348 2.97e-127 372
MS.gene00462.t1 MTR_3g075390 51.261 357 164 7 11 365 6 354 9.47e-124 363
MS.gene00462.t1 MTR_4g104370 49.862 363 172 7 13 373 6 360 2.41e-118 350
MS.gene00462.t1 MTR_2g016180 45.388 412 200 8 42 438 43 444 7.57e-116 348
MS.gene00462.t1 MTR_2g016270 44.878 410 190 8 42 438 43 429 1.55e-113 342
MS.gene00462.t1 MTR_4g079470 51.227 326 142 7 37 355 28 343 1.11e-112 335
MS.gene00462.t1 MTR_4g079770 53.035 313 130 7 50 355 41 343 1.51e-112 334
MS.gene00462.t1 MTR_5g022560 48.864 352 151 8 16 355 8 342 4.18e-112 333
MS.gene00462.t1 MTR_4g079800 52.396 313 132 7 50 355 41 343 4.81e-111 331
MS.gene00462.t1 MTR_4g047610 50.462 325 145 6 38 355 26 341 5.42e-111 330
MS.gene00462.t1 MTR_2g075830 48.980 343 153 7 19 355 13 339 3.86e-110 328
MS.gene00462.t1 MTR_4g080730 52.597 308 135 6 50 355 40 338 2.03e-108 324
MS.gene00462.t1 MTR_4g080700 51.948 308 137 6 50 355 40 338 4.06e-107 320
MS.gene00462.t1 MTR_4g079440 45.559 349 179 6 10 356 5 344 8.69e-107 320
MS.gene00462.t1 MTR_2g090890 46.307 352 166 7 14 358 3 338 1.32e-106 319
MS.gene00462.t1 MTR_4g107930 50.467 321 140 8 45 355 32 343 1.78e-106 319
MS.gene00462.t1 MTR_4g080360 47.664 321 161 6 39 356 25 341 1.56e-100 304
MS.gene00462.t1 MTR_2g083930 45.342 322 172 4 38 356 23 343 2.47e-98 298
MS.gene00462.t1 MTR_7g078600 46.326 313 149 10 50 358 42 339 2.34e-86 267
MS.gene00462.t1 MTR_7g079230 43.077 325 166 8 37 355 23 334 1.55e-85 265
MS.gene00462.t1 MTR_2g015850 47.797 295 145 7 64 356 76 363 6.98e-81 254
MS.gene00462.t1 MTR_4g065077 40.872 367 183 14 5 357 11 357 1.91e-76 243
MS.gene00462.t1 MTR_2g015920 37.228 368 207 12 9 362 8 365 5.45e-71 229
MS.gene00462.t1 MTR_2g015930 38.950 362 195 13 11 357 9 359 1.81e-69 224
MS.gene00462.t1 MTR_4g125300 38.227 361 190 12 11 356 6 348 3.65e-67 218
MS.gene00462.t1 MTR_2g067900 42.902 317 165 11 50 356 16 326 1.50e-66 216
MS.gene00462.t1 MTR_8g086470 37.741 363 184 13 5 357 27 357 2.26e-66 216
MS.gene00462.t1 MTR_1g023210 41.611 298 148 8 56 336 57 345 1.63e-63 209
MS.gene00462.t1 MTR_4g071060 38.983 354 168 12 13 336 6 341 2.00e-63 209
MS.gene00462.t1 MTR_2g067560 39.255 349 186 15 48 380 14 352 6.92e-55 196
MS.gene00462.t1 MTR_1g094135 33.962 371 201 10 4 348 6 358 7.44e-55 187
MS.gene00462.t1 MTR_2g064475 37.101 345 169 14 48 354 14 348 1.78e-54 185
MS.gene00462.t1 MTR_1g023810 45.249 221 111 7 138 354 47 261 1.50e-52 177
MS.gene00462.t1 MTR_2g066980 32.861 353 183 12 19 355 3 317 8.37e-51 174
MS.gene00462.t1 MTR_7g078630 37.162 296 134 8 28 311 690 945 2.78e-48 177
MS.gene00462.t1 MTR_4g072790 35.714 294 146 8 50 336 48 305 3.69e-46 162
MS.gene00462.t1 MTR_4g072340 32.551 341 179 10 7 336 9 309 3.73e-44 157
MS.gene00462.t1 MTR_2g016380 41.148 209 111 4 240 438 18 224 5.32e-44 154
MS.gene00462.t1 MTR_4g073660 34.459 296 151 7 50 336 47 308 5.52e-44 157
MS.gene00462.t1 MTR_1g025450 43.103 174 77 5 138 308 53 207 5.59e-33 131
MS.gene00462.t1 MTR_7g078580 50.476 105 49 3 258 361 4 106 4.15e-29 110
MS.gene00462.t1 MTR_2g015840 39.259 135 78 2 42 175 25 156 1.18e-27 111
MS.gene00462.t1 MTR_2g015840 44.954 109 57 3 248 355 164 270 8.87e-25 103
MS.gene00462.t1 MTR_7g079130 50.538 93 44 2 265 356 6 97 8.04e-25 98.2
MS.gene00462.t1 MTR_3g029560 34.286 210 113 8 151 356 49 237 2.15e-21 93.2
MS.gene00462.t1 MTR_7g078310 47.917 96 47 2 140 232 152 247 8.27e-21 92.0
MS.gene00462.t1 MTR_7g111060 27.758 281 158 12 97 348 66 330 4.73e-20 91.7
MS.gene00462.t1 MTR_2g047960 48.101 79 41 0 149 227 7 85 2.44e-18 80.5
MS.gene00462.t1 MTR_7g111050 26.715 277 166 11 97 348 63 327 4.34e-17 82.8
MS.gene00462.t1 MTR_8g087510 33.588 131 79 7 181 308 23 148 5.40e-12 64.7
MS.gene00462.t1 MTR_7g079260 42.466 73 24 2 282 353 7 62 6.41e-11 59.7
MS.gene00462.t1 MTR_1g009480 27.451 102 66 1 5 106 1 94 7.24e-11 60.5
MS.gene00462.t1 MTR_7g111050 26.016 246 150 10 97 317 58 296 9.56e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00462.t1 AT1G47128 72.979 433 107 4 8 437 8 433 0.0 625
MS.gene00462.t1 AT5G43060 72.055 433 110 5 9 437 9 434 0.0 610
MS.gene00462.t1 AT3G19390 60.325 431 150 8 9 436 8 420 0.0 516
MS.gene00462.t1 AT4G36880 62.195 328 118 2 41 362 40 367 1.96e-151 435
MS.gene00462.t1 AT1G09850 52.645 397 173 5 44 438 26 409 3.20e-143 416
MS.gene00462.t1 AT3G19400 59.133 323 122 6 40 358 34 350 8.56e-137 397
MS.gene00462.t1 AT4G23520 55.028 358 142 8 5 358 9 351 4.74e-136 395
MS.gene00462.t1 AT4G11310 51.524 361 165 6 1 358 1 354 3.10e-127 373
MS.gene00462.t1 AT4G11320 51.801 361 164 6 3 358 6 361 5.00e-126 370
MS.gene00462.t1 AT4G11320 51.801 361 164 6 3 358 6 361 5.00e-126 370
MS.gene00462.t1 AT3G48340 51.831 355 164 5 12 365 4 352 5.86e-125 367
MS.gene00462.t1 AT1G20850 54.167 336 142 5 23 357 30 354 9.79e-123 361
MS.gene00462.t1 AT4G35350 50.142 351 164 3 7 357 14 353 1.42e-121 358
MS.gene00462.t1 AT5G50260 55.521 317 135 5 49 363 37 349 5.52e-119 352
MS.gene00462.t1 AT1G06260 47.714 350 164 6 11 357 10 343 1.91e-109 327
MS.gene00462.t1 AT3G48350 47.790 362 172 7 5 362 1 349 1.14e-108 325
MS.gene00462.t1 AT5G45890 45.042 353 173 6 7 355 9 344 1.23e-105 317
MS.gene00462.t1 AT3G43960 51.327 339 150 11 37 368 28 358 4.03e-103 311
MS.gene00462.t1 AT3G19400 58.964 251 94 5 40 286 34 279 2.76e-101 304
MS.gene00462.t1 AT2G34080 42.033 364 183 9 1 356 1 344 3.39e-91 280
MS.gene00462.t1 AT2G27420 42.936 361 183 10 5 356 1 347 8.56e-91 279
MS.gene00462.t1 AT3G49340 46.815 314 152 9 50 356 35 340 1.88e-90 278
MS.gene00462.t1 AT4G35350 47.535 284 138 3 7 290 14 286 4.70e-89 272
MS.gene00462.t1 AT1G29080 41.905 315 175 7 46 356 35 345 4.43e-82 256
MS.gene00462.t1 AT1G29090 40.000 365 179 11 9 356 13 354 1.10e-81 256
MS.gene00462.t1 AT3G48350 44.097 288 148 5 5 290 1 277 6.30e-74 234
MS.gene00462.t1 AT1G29110 35.673 342 202 6 19 356 6 333 6.87e-70 224
MS.gene00462.t1 AT5G60360 38.000 350 180 12 27 356 24 356 9.81e-66 214
MS.gene00462.t1 AT3G45310 36.000 375 202 12 1 356 1 356 1.00e-64 212
MS.gene00462.t1 AT4G39090 39.748 317 148 7 42 334 46 343 2.45e-64 211
MS.gene00462.t1 AT5G60360 37.429 350 181 12 27 356 24 355 1.82e-63 209
MS.gene00462.t1 AT2G21430 36.212 359 185 10 1 334 1 340 3.85e-63 208
MS.gene00462.t1 AT5G60360 38.226 327 174 10 27 335 24 340 5.24e-63 207
MS.gene00462.t1 AT3G45310 35.544 377 200 13 1 356 1 355 3.22e-62 206
MS.gene00462.t1 AT4G16190 35.574 357 195 11 6 336 3 350 1.98e-57 193
MS.gene00462.t1 AT3G54940 33.053 357 196 10 6 336 8 347 1.17e-51 178
MS.gene00462.t1 AT3G54940 32.042 284 161 7 6 272 8 276 4.30e-39 142
MS.gene00462.t1 AT4G01610 27.491 291 172 12 97 361 66 343 1.52e-20 93.2
MS.gene00462.t1 AT1G02305 27.698 278 162 11 97 348 69 333 5.73e-20 91.3
MS.gene00462.t1 AT4G01610 26.804 291 174 12 97 361 66 343 1.02e-18 87.8
MS.gene00462.t1 AT1G02300 28.095 210 122 8 161 348 148 350 3.35e-13 71.2

Find 117 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATGGGAAAGATTAT+TGG 0.227075 1.4:-4271348 MS.gene00462:CDS
GAACCGTAGGATGAAGAAAT+TGG 0.244550 1.4:-4273905 MS.gene00462:CDS
TATGTTCATCAATGAATTTA+AGG 0.255434 1.4:+4274025 None:intergenic
GTTGGATACGGTACAGAAAA+TGG 0.255932 1.4:-4271361 MS.gene00462:CDS
GGTGGTGATGGTCCAGGGTT+AGG 0.264790 1.4:+4271187 None:intergenic
GGGTTCTGTCCATTCTTAAT+TGG 0.270191 1.4:+4271211 None:intergenic
TTCCTCCAATCAACAGATTC+AGG 0.289567 1.4:+4273835 None:intergenic
TGTTGCTGTATTTACGAGTA+TGG 0.314590 1.4:-4271103 MS.gene00462:CDS
TTTCTCACCTAAACCGTTGT+AGG 0.323971 1.4:+4274077 None:intergenic
AACTTACATATTCATATAAC+TGG 0.326706 1.4:+4271718 None:intergenic
AGGATGCAATGGTGGACTTA+TGG 0.331252 1.4:-4272097 MS.gene00462:CDS
TGAATTCGACATATAGATTA+GGG 0.336748 1.4:-4273996 MS.gene00462:CDS
GTGGTGATGGTCCAGGGTTA+GGG 0.341154 1.4:+4271188 None:intergenic
GGAAAATCCTACAACGGTTT+AGG 0.345309 1.4:-4274084 MS.gene00462:CDS
ATGGTAGATGTGACCAGAAC+AGG 0.354867 1.4:-4272002 MS.gene00462:CDS
GAAATTCATGGGGTGGTAGC+TGG 0.356281 1.4:-4271318 MS.gene00462:CDS
TTGAATTCGACATATAGATT+AGG 0.369544 1.4:-4273997 MS.gene00462:CDS
CGTTAGTTAGATCCGCGAAC+CGG 0.375000 1.4:+4273968 None:intergenic
CAGACAGATGGTGGCTTGAT+AGG 0.385214 1.4:+4271160 None:intergenic
TGTTCCTGCTTATGATGAAT+TGG 0.389594 1.4:-4271805 MS.gene00462:CDS
TCTTGTCAGGGTGTATTTAC+CGG 0.390275 1.4:-4271421 MS.gene00462:intron
CCTATCGCCGTTGCTGTTGA+AGG 0.401421 1.4:-4271756 MS.gene00462:CDS
ATTGGATTGTGAGAAATTCA+TGG 0.410060 1.4:-4271330 MS.gene00462:CDS
CCAAGTAACTAACCCTGTTC+TGG 0.415431 1.4:+4271989 None:intergenic
TACCCTTACAAGGCTATTGA+TGG 0.421160 1.4:-4272021 MS.gene00462:CDS
CTACCATCAATAGCCTTGTA+AGG 0.422671 1.4:+4272018 None:intergenic
AATTGCCTGAATCTGTTGAT+TGG 0.426885 1.4:-4273840 MS.gene00462:CDS
CCATTCTTAATTGGATAAGA+TGG 0.437304 1.4:+4271220 None:intergenic
AAATTCATGGGGTGGTAGCT+GGG 0.440858 1.4:-4271317 MS.gene00462:CDS
TCAGCAATCGCAGCTGTTGA+AGG 0.443055 1.4:-4272198 MS.gene00462:CDS
TTTCAATGAAGGGAGTTGTT+GGG 0.446061 1.4:-4272226 MS.gene00462:intron
GGTGATGGTCCAGGGTTAGG+GGG 0.448618 1.4:+4271190 None:intergenic
TGGTGATGGTCCAGGGTTAG+GGG 0.449337 1.4:+4271189 None:intergenic
GTGATGGTCCAGGGTTAGGG+GGG 0.453507 1.4:+4271191 None:intergenic
CATAGTCAACACTTCTTCGT+TGG 0.459007 1.4:+4274131 None:intergenic
AGGAAGGAAGGTGCTGTTGT+TGG 0.459612 1.4:-4273817 MS.gene00462:CDS
TCGTGCTGGACTTTGTCTTA+AGG 0.462543 1.4:-4270981 None:intergenic
TTCACTATCAGAACAAGAAC+TGG 0.467996 1.4:-4272145 MS.gene00462:CDS
AATTTCTTCATCCTACGGTT+CGG 0.472057 1.4:+4273907 None:intergenic
GGTGAAGGTGGTGATGGTCC+AGG 0.475487 1.4:+4271181 None:intergenic
TGATGAATTGGCCTTGCAAA+AGG 0.476220 1.4:-4271793 MS.gene00462:CDS
GGAATAAATAAGATTGTGAC+AGG 0.481755 1.4:-4272177 MS.gene00462:CDS
AGGAGATGATCGACATGTCT+AGG 0.481788 1.4:+4274193 None:intergenic
TGAAGAAATTGGGTGGTGTA+AGG 0.482021 1.4:-4273894 MS.gene00462:CDS
CACCCAATTTCTTCATCCTA+CGG 0.483167 1.4:+4273902 None:intergenic
AGTGCTTACCACAACTTGCT+TGG 0.487527 1.4:+4273785 None:intergenic
GCTTGTTGATTTGTCTGGGT+GGG 0.490913 1.4:+4274161 None:intergenic
CAACAGCAACGGCGATAGGC+TGG 0.494154 1.4:+4271760 None:intergenic
ATTAGGGTTGACCCGGTTCG+CGG 0.494343 1.4:-4273980 MS.gene00462:CDS
CCTTCAACAGCAACGGCGAT+AGG 0.495351 1.4:+4271756 None:intergenic
AACCGTAGGATGAAGAAATT+GGG 0.496972 1.4:-4273904 MS.gene00462:CDS
TTGGATACGGTACAGAAAAT+GGG 0.502646 1.4:-4271360 MS.gene00462:CDS
CCTGTCTGTGACACTCGTGC+TGG 0.506173 1.4:-4270995 MS.gene00462:CDS
TGGAAGATCTTGCTTTGAGT+GGG 0.510888 1.4:-4271083 MS.gene00462:CDS
CCATCTTATCCAATTAAGAA+TGG 0.514473 1.4:-4271220 MS.gene00462:CDS
TGCTTGTTGATTTGTCTGGG+TGG 0.529438 1.4:+4274160 None:intergenic
AAATTCATTGATGAACATAA+CGG 0.530458 1.4:-4274021 MS.gene00462:CDS
TCAAGCAGAGCAGGAAAGTG+TGG 0.532736 1.4:-4271256 MS.gene00462:CDS
GGCTAGTGAAACTGTGAAAG+TGG 0.534429 1.4:+4274215 None:intergenic
GTTAGTTAGATCCGCGAACC+GGG 0.534519 1.4:+4273969 None:intergenic
AGCTACTACAGCTGTGCTGA+AGG 0.535391 1.4:-4271133 MS.gene00462:CDS
TTTGAGTTCATCATCAGTAA+TGG 0.537702 1.4:-4272066 MS.gene00462:CDS
AATCTAGCTTCAAGCAGAGC+AGG 0.546872 1.4:-4271265 MS.gene00462:CDS
TTGGATTGTGAGAAATTCAT+GGG 0.548066 1.4:-4271329 MS.gene00462:CDS
GACCATGGTGTTGCAGCTGT+TGG 0.548818 1.4:-4271379 MS.gene00462:CDS
TGCCTGAATCTGTTGATTGG+AGG 0.549740 1.4:-4273837 MS.gene00462:CDS
TGCTGTTGAAGGAGGCGGTA+GGG 0.550318 1.4:-4271745 MS.gene00462:CDS
GGTGGTAGCTGGGGTGAACA+AGG 0.554597 1.4:-4271307 MS.gene00462:CDS
TTGATAGGTGAAGGTGGTGA+TGG 0.555756 1.4:+4271175 None:intergenic
GCCGTTGCTGTTGAAGGAGG+CGG 0.557434 1.4:-4271750 MS.gene00462:CDS
GGTGTTGCAGCTGTTGGATA+CGG 0.561703 1.4:-4271373 MS.gene00462:CDS
CATATAGATTAGGGTTGACC+CGG 0.561757 1.4:-4273987 MS.gene00462:CDS
ATGGAAGATCTTGCTTTGAG+TGG 0.563923 1.4:-4271084 MS.gene00462:CDS
TAGTGCCGTTCCACATCTAC+CGG 0.569067 1.4:+4271402 None:intergenic
CCAGCACGAGTGTCACAGAC+AGG 0.571924 1.4:+4270995 None:intergenic
TGAAGAGGATTACCCTTACA+AGG 0.572769 1.4:-4272031 MS.gene00462:CDS
AATTCATGGGGTGGTAGCTG+GGG 0.574383 1.4:-4271316 MS.gene00462:CDS
GGAGATGATCGACATGTCTA+GGG 0.575045 1.4:+4274194 None:intergenic
AATCGTTATGCGCCACGTGT+CGG 0.576425 1.4:-4273868 MS.gene00462:CDS
GTTTAGGTGAGAAAGACAAA+AGG 0.579754 1.4:-4274068 MS.gene00462:CDS
GTCAAAGACCAAGCAAGTTG+TGG 0.584862 1.4:-4273793 MS.gene00462:intron
AAGGCCAATTCATCATAAGC+AGG 0.585009 1.4:+4271801 None:intergenic
GGTGTATTTACCGGTAGATG+TGG 0.590389 1.4:-4271412 MS.gene00462:CDS
TACCATCAATAGCCTTGTAA+GGG 0.599159 1.4:+4272019 None:intergenic
GAGTTCATCATCAGTAATGG+TGG 0.599792 1.4:-4272063 MS.gene00462:CDS
ACTATCAGAACAAGAACTGG+TGG 0.602270 1.4:-4272142 MS.gene00462:CDS
GTGAAGGTGGTGATGGTCCA+GGG 0.603987 1.4:+4271182 None:intergenic
ATCGCCGTTGCTGTTGAAGG+AGG 0.609464 1.4:-4271753 MS.gene00462:CDS
TTGCTGTTGAAGGAGGCGGT+AGG 0.609931 1.4:-4271746 MS.gene00462:CDS
GAAGAGTGGTTAGTGAAACA+TGG 0.615801 1.4:-4274105 MS.gene00462:CDS
CAAAGATCGATCCGAACCGT+AGG 0.618623 1.4:-4273918 MS.gene00462:CDS
CAGCAAGTAGCAGACTCAAG+AGG 0.620435 1.4:+4271052 None:intergenic
TAGTAGCTGTCACAGACAGA+TGG 0.620790 1.4:+4271148 None:intergenic
TGGTAGATGTGACCAGAACA+GGG 0.623188 1.4:-4272001 MS.gene00462:intron
TGGTGGCATTGACTCTGAAG+AGG 0.626193 1.4:-4272046 MS.gene00462:CDS
TCATACAACGAAGGATGCAA+TGG 0.627683 1.4:-4272108 MS.gene00462:CDS
TACAACGAAGGATGCAATGG+TGG 0.630463 1.4:-4272105 MS.gene00462:CDS
GGCAATTTATCACCGACACG+TGG 0.638153 1.4:+4273856 None:intergenic
TGTTGACTATGTACGAAGAG+TGG 0.640568 1.4:-4274119 MS.gene00462:CDS
TAGCTGTCACAGACAGATGG+TGG 0.645708 1.4:+4271151 None:intergenic
ATTACTGATGATGAACTCAA+AGG 0.646757 1.4:+4272068 None:intergenic
GGTGGCTTGATAGGTGAAGG+TGG 0.647988 1.4:+4271169 None:intergenic
ACCGCCTCCTTCAACAGCAA+CGG 0.651172 1.4:+4271749 None:intergenic
TCTGTTGATTGGAGGAAGGA+AGG 0.651628 1.4:-4273829 MS.gene00462:CDS
TGAATCTGTTGATTGGAGGA+AGG 0.656666 1.4:-4273833 MS.gene00462:CDS
CACAGACAGGATACTCATGA+GGG 0.662230 1.4:+4271008 None:intergenic
TCACAGACAGGATACTCATG+AGG 0.662392 1.4:+4271007 None:intergenic
ATTTACCGGTAGATGTGGAA+CGG 0.675984 1.4:-4271407 MS.gene00462:CDS
ATCCAACAGCTGCAACACCA+TGG 0.676166 1.4:+4271377 None:intergenic
TGTGGAACGGCACTAGACCA+TGG 0.678729 1.4:-4271394 MS.gene00462:CDS
CGTAGGATGAAGAAATTGGG+TGG 0.680087 1.4:-4273901 MS.gene00462:CDS
GATGGTGGCTTGATAGGTGA+AGG 0.689206 1.4:+4271166 None:intergenic
GGAAGATCTTGCTTTGAGTG+GGG 0.689687 1.4:-4271082 MS.gene00462:CDS
AAACATGGAAAATCCTACAA+CGG 0.700433 1.4:-4274090 MS.gene00462:CDS
ATTGTGAGAAATTCATGGGG+TGG 0.703535 1.4:-4271325 MS.gene00462:CDS
TGTGATACATCATACAACGA+AGG 0.706999 1.4:-4272117 MS.gene00462:CDS
TGGATTGTGAGAAATTCATG+GGG 0.737069 1.4:-4271328 MS.gene00462:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAATACAAAAAAAAAAA+AGG + chr1.4:4272340-4272359 None:intergenic 10.0%
!!! ACAAAATTTTAATTCATAAA+AGG - chr1.4:4272030-4272049 MS.gene00462:CDS 10.0%
!!! CAAAATTTTAATTCATAAAA+GGG - chr1.4:4272031-4272050 MS.gene00462:CDS 10.0%
!! GTCAAAAAAAATAAATTAAC+AGG + chr1.4:4273727-4273746 None:intergenic 15.0%
!! TTAAACTAAAAACAATTCTT+TGG - chr1.4:4272293-4272312 MS.gene00462:intron 15.0%
!!! AACAAAAAAGCATTATATTA+TGG + chr1.4:4272807-4272826 None:intergenic 15.0%
!!! ATAATATTAGCAATTTTTTG+TGG + chr1.4:4272983-4273002 None:intergenic 15.0%
!!! TTAGATTTTTCTTATTTTCT+TGG - chr1.4:4271840-4271859 MS.gene00462:CDS 15.0%
!! AAATTCATTGATGAACATAA+CGG - chr1.4:4271216-4271235 MS.gene00462:CDS 20.0%
!! AACTTACATATTCATATAAC+TGG + chr1.4:4273522-4273541 None:intergenic 20.0%
!! ACAATACACCAAAAAATTAT+GGG + chr1.4:4272104-4272123 None:intergenic 20.0%
!! ATTAATTCTACTATGAAACT+AGG - chr1.4:4273547-4273566 MS.gene00462:intron 20.0%
!! TACAATACACCAAAAAATTA+TGG + chr1.4:4272105-4272124 None:intergenic 20.0%
!! TATGTTCATCAATGAATTTA+AGG + chr1.4:4271215-4271234 None:intergenic 20.0%
!! TATTCAAAATCAATATGTGA+AGG + chr1.4:4273645-4273664 None:intergenic 20.0%
!!! ATCTTCTTTTTTTCAATGAA+GGG - chr1.4:4273001-4273020 MS.gene00462:intron 20.0%
!!! ATTTTCTTGGATATGTTATT+AGG - chr1.4:4271853-4271872 MS.gene00462:intron 20.0%
!!! TAATGATGTACTTTAATTTG+TGG - chr1.4:4271760-4271779 MS.gene00462:CDS 20.0%
!!! TATATTTCGTATGACTTTTA+AGG + chr1.4:4272581-4272600 None:intergenic 20.0%
!!! TATCTTCTTTTTTTCAATGA+AGG - chr1.4:4273000-4273019 MS.gene00462:intron 20.0%
! AATCAAACTCAGTTAATCAT+GGG + chr1.4:4272905-4272924 None:intergenic 25.0%
! ATGACAATAGTAAATGTTTG+TGG - chr1.4:4273285-4273304 MS.gene00462:intron 25.0%
! ATTGATATATTGAGTACTCT+TGG + chr1.4:4272755-4272774 None:intergenic 25.0%
! CAATCATTGGTGTAATAATA+TGG - chr1.4:4272771-4272790 MS.gene00462:intron 25.0%
! GTACTCAATATATCAATCAT+TGG - chr1.4:4272758-4272777 MS.gene00462:intron 25.0%
! TAACACTCTAATTCTTTCAT+TGG - chr1.4:4273337-4273356 MS.gene00462:intron 25.0%
! TAATCAAACTCAGTTAATCA+TGG + chr1.4:4272906-4272925 None:intergenic 25.0%
! TGAATTCGACATATAGATTA+GGG - chr1.4:4271241-4271260 MS.gene00462:CDS 25.0%
! TGATTAACTGAGTTTGATTA+AGG - chr1.4:4272906-4272925 MS.gene00462:intron 25.0%
! TGCATTTGTTTGTTCAATAT+GGG - chr1.4:4272482-4272501 MS.gene00462:intron 25.0%
! TTGAATAAAAATCATCTGTC+TGG - chr1.4:4273658-4273677 MS.gene00462:intron 25.0%
! TTGAATTCGACATATAGATT+AGG - chr1.4:4271240-4271259 MS.gene00462:CDS 25.0%
! TTGATATATTGAGTACTCTT+GGG + chr1.4:4272754-4272773 None:intergenic 25.0%
! TTGCATTTGTTTGTTCAATA+TGG - chr1.4:4272481-4272500 MS.gene00462:intron 25.0%
!! AATTTTATACTGTGTTGCAT+TGG - chr1.4:4273570-4273589 MS.gene00462:intron 25.0%
!! ATTTTATACTGTGTTGCATT+GGG - chr1.4:4273571-4273590 MS.gene00462:intron 25.0%
!! TTAAGGAACATGATAGTTTT+TGG - chr1.4:4272923-4272942 MS.gene00462:intron 25.0%
!! TTTAAGGTAGACATGATTAA+AGG + chr1.4:4272565-4272584 None:intergenic 25.0%
!!! ATTTTAATGATCTGAGAGTT+AGG + chr1.4:4272218-4272237 None:intergenic 25.0%
!!! CTTTGTTTTGTAGTCATATA+AGG - chr1.4:4272372-4272391 MS.gene00462:intron 25.0%
!!! TGATTTAAAATTTTGTGCAC+CGG + chr1.4:4272171-4272190 None:intergenic 25.0%
!!! TTCATTGGTTTTAAACTGTT+TGG - chr1.4:4273352-4273371 MS.gene00462:intron 25.0%
AAACATGGAAAATCCTACAA+CGG - chr1.4:4271147-4271166 MS.gene00462:CDS 30.0%
ACGTAGATAATCATGTAACA+TGG - chr1.4:4272693-4272712 MS.gene00462:intron 30.0%
ATACAATACACCAAGAAACT+GGG + chr1.4:4272138-4272157 None:intergenic 30.0%
ATCAAACTCAGTTAATCATG+GGG + chr1.4:4272904-4272923 None:intergenic 30.0%
ATCCATGTGTAACAATATCT+GGG - chr1.4:4271720-4271739 MS.gene00462:intron 30.0%
ATGAAATCCCAAAGCAAATA+AGG + chr1.4:4271959-4271978 None:intergenic 30.0%
ATTGGATTGTGAGAAATTCA+TGG - chr1.4:4273907-4273926 MS.gene00462:CDS 30.0%
CAGAAAATGGGAAAGATTAT+TGG - chr1.4:4273889-4273908 MS.gene00462:CDS 30.0%
CCATCTTATCCAATTAAGAA+TGG - chr1.4:4274017-4274036 MS.gene00462:CDS 30.0%
CCATGTTATGATTGATTGAT+AGG + chr1.4:4272529-4272548 None:intergenic 30.0%
CCATTCTTAATTGGATAAGA+TGG + chr1.4:4274020-4274039 None:intergenic 30.0%
CCTATCAATCAATCATAACA+TGG - chr1.4:4272526-4272545 MS.gene00462:intron 30.0%
GCAAATCAAAATCAATCGTA+TGG + chr1.4:4272265-4272284 None:intergenic 30.0%
GGAATAAATAAGATTGTGAC+AGG - chr1.4:4273060-4273079 MS.gene00462:intron 30.0%
TACTTCTGCTCAAGAAATAA+AGG + chr1.4:4272850-4272869 None:intergenic 30.0%
TTGGATTGTGAGAAATTCAT+GGG - chr1.4:4273908-4273927 MS.gene00462:CDS 30.0%
! AGAGTATAGAAGCAAGTTTT+TGG - chr1.4:4271293-4271312 MS.gene00462:CDS 30.0%
! ATTACTGATGATGAACTCAA+AGG + chr1.4:4273172-4273191 None:intergenic 30.0%
! GAGTATAGAAGCAAGTTTTT+GGG - chr1.4:4271294-4271313 MS.gene00462:CDS 30.0%
! TGGATTTCCTTATTTGCTTT+GGG - chr1.4:4271949-4271968 MS.gene00462:intron 30.0%
! TTTGAGTTCATCATCAGTAA+TGG - chr1.4:4273171-4273190 MS.gene00462:intron 30.0%
!! GATTTAAAATTTTGTGCACC+GGG + chr1.4:4272170-4272189 None:intergenic 30.0%
!!! TTTTGCTTGTTGATTTGTCT+GGG + chr1.4:4271083-4271102 None:intergenic 30.0%
AACCGTAGGATGAAGAAATT+GGG - chr1.4:4271333-4271352 MS.gene00462:CDS 35.0%
AATTGCCTGAATCTGTTGAT+TGG - chr1.4:4271397-4271416 MS.gene00462:CDS 35.0%
AATTTCTTCATCCTACGGTT+CGG + chr1.4:4271333-4271352 None:intergenic 35.0%
ATTCCATTTGTCTCTTGTCA+GGG - chr1.4:4273804-4273823 MS.gene00462:CDS 35.0%
CAAATGCAAACCAAGAATTC+TGG + chr1.4:4272470-4272489 None:intergenic 35.0%
CAGATATTGTTACACATGGA+TGG + chr1.4:4271721-4271740 None:intergenic 35.0%
CATACAATACACCAAGAAAC+TGG + chr1.4:4272139-4272158 None:intergenic 35.0%
CATCCATGTGTAACAATATC+TGG - chr1.4:4271719-4271738 MS.gene00462:intron 35.0%
CCTAAAATCACCAGAATTCT+TGG - chr1.4:4272457-4272476 MS.gene00462:intron 35.0%
CTTGATCCTGATAATTCATC+TGG - chr1.4:4271929-4271948 MS.gene00462:intron 35.0%
GGAAATCCAGATGAATTATC+AGG + chr1.4:4271938-4271957 None:intergenic 35.0%
GGCTATTGAAGATAATGTGA+AGG + chr1.4:4273706-4273725 None:intergenic 35.0%
GTAGTCATATAAGGTCTGTT+TGG - chr1.4:4272381-4272400 MS.gene00462:intron 35.0%
GTGGAAGATAGTTAATCAGT+AGG - chr1.4:4273304-4273323 MS.gene00462:intron 35.0%
GTTTAGGTGAGAAAGACAAA+AGG - chr1.4:4271169-4271188 MS.gene00462:CDS 35.0%
TATTCCATTTGTCTCTTGTC+AGG - chr1.4:4273803-4273822 MS.gene00462:CDS 35.0%
TGGATTGTGAGAAATTCATG+GGG - chr1.4:4273909-4273928 MS.gene00462:CDS 35.0%
TGTGATACATCATACAACGA+AGG - chr1.4:4273120-4273139 MS.gene00462:intron 35.0%
TGTTCCTGCTTATGATGAAT+TGG - chr1.4:4273432-4273451 MS.gene00462:intron 35.0%
TGTTGCTGTATTTACGAGTA+TGG - chr1.4:4274134-4274153 MS.gene00462:CDS 35.0%
TTAATGATCTGAGAGTTAGG+AGG + chr1.4:4272215-4272234 None:intergenic 35.0%
TTCACTATCAGAACAAGAAC+TGG - chr1.4:4273092-4273111 MS.gene00462:intron 35.0%
TTCCCAGATATTGTTACACA+TGG + chr1.4:4271725-4271744 None:intergenic 35.0%
TTGACTTTGCTTCACAAGAT+TGG - chr1.4:4273742-4273761 MS.gene00462:intron 35.0%
TTGGATACGGTACAGAAAAT+GGG - chr1.4:4273877-4273896 MS.gene00462:CDS 35.0%
TTTCAATGAAGGGAGTTGTT+GGG - chr1.4:4273011-4273030 MS.gene00462:intron 35.0%
TTTGGATTTCGTTTGCAGTA+TGG - chr1.4:4272311-4272330 MS.gene00462:intron 35.0%
! CCAAGAATTCTGGTGATTTT+AGG + chr1.4:4272460-4272479 None:intergenic 35.0%
! CCTTAAAAGTTTTACGACGA+CGG - chr1.4:4272622-4272641 MS.gene00462:intron 35.0%
! CTGGATTTCCTTATTTGCTT+TGG - chr1.4:4271948-4271967 MS.gene00462:intron 35.0%
! GTTTCTTGGTGTATTGTATG+TGG - chr1.4:4272139-4272158 MS.gene00462:CDS 35.0%
! TTTTCAATGAAGGGAGTTGT+TGG - chr1.4:4273010-4273029 MS.gene00462:intron 35.0%
!! TACCATCAATAGCCTTGTAA+GGG + chr1.4:4273221-4273240 None:intergenic 35.0%
!!! CTTTTGCTTGTTGATTTGTC+TGG + chr1.4:4271084-4271103 None:intergenic 35.0%
AAAAATTATGGGACCCTTCC+CGG + chr1.4:4272093-4272112 None:intergenic 40.0%
AAAATCATCTGTCTGGCAAG+TGG - chr1.4:4273665-4273684 MS.gene00462:intron 40.0%
AAGGCCAATTCATCATAAGC+AGG + chr1.4:4273439-4273458 None:intergenic 40.0%
ACTATCAGAACAAGAACTGG+TGG - chr1.4:4273095-4273114 MS.gene00462:intron 40.0%
ATTGTGAGAAATTCATGGGG+TGG - chr1.4:4273912-4273931 MS.gene00462:CDS 40.0%
ATTTACCGGTAGATGTGGAA+CGG - chr1.4:4273830-4273849 MS.gene00462:CDS 40.0%
CACCCAATTTCTTCATCCTA+CGG + chr1.4:4271338-4271357 None:intergenic 40.0%
CATAGTCAACACTTCTTCGT+TGG + chr1.4:4271109-4271128 None:intergenic 40.0%
CATATAGATTAGGGTTGACC+CGG - chr1.4:4271250-4271269 MS.gene00462:CDS 40.0%
GAACCGTAGGATGAAGAAAT+TGG - chr1.4:4271332-4271351 MS.gene00462:CDS 40.0%
GAAGAGTGGTTAGTGAAACA+TGG - chr1.4:4271132-4271151 MS.gene00462:CDS 40.0%
GAGTTCATCATCAGTAATGG+TGG - chr1.4:4273174-4273193 MS.gene00462:intron 40.0%
GGAAAATCCTACAACGGTTT+AGG - chr1.4:4271153-4271172 MS.gene00462:CDS 40.0%
GGGTTCTGTCCATTCTTAAT+TGG + chr1.4:4274029-4274048 None:intergenic 40.0%
GTTGGATACGGTACAGAAAA+TGG - chr1.4:4273876-4273895 MS.gene00462:CDS 40.0%
TAAAACTCCCCGCATAAGTA+GGG - chr1.4:4272066-4272085 MS.gene00462:CDS 40.0%
TACCCTTACAAGGCTATTGA+TGG - chr1.4:4273216-4273235 MS.gene00462:intron 40.0%
TCATACAACGAAGGATGCAA+TGG - chr1.4:4273129-4273148 MS.gene00462:intron 40.0%
TCTTGTCAGGGTGTATTTAC+CGG - chr1.4:4273816-4273835 MS.gene00462:CDS 40.0%
TGAAGAAATTGGGTGGTGTA+AGG - chr1.4:4271343-4271362 MS.gene00462:CDS 40.0%
TGAAGAGGATTACCCTTACA+AGG - chr1.4:4273206-4273225 MS.gene00462:intron 40.0%
TGTAAGATATCCATCAGCTG+TGG + chr1.4:4272437-4272456 None:intergenic 40.0%
TTCCTCCAATCAACAGATTC+AGG + chr1.4:4271405-4271424 None:intergenic 40.0%
TTTCTCACCTAAACCGTTGT+AGG + chr1.4:4271163-4271182 None:intergenic 40.0%
! AAAATTTTGTGCACCGGGTA+AGG + chr1.4:4272165-4272184 None:intergenic 40.0%
! ATGGAAGATCTTGCTTTGAG+TGG - chr1.4:4274153-4274172 MS.gene00462:CDS 40.0%
! CCGTCGTCGTAAAACTTTTA+AGG + chr1.4:4272625-4272644 None:intergenic 40.0%
! TGAATCTGTTGATTGGAGGA+AGG - chr1.4:4271404-4271423 MS.gene00462:CDS 40.0%
! TGGAAGATCTTGCTTTGAGT+GGG - chr1.4:4274154-4274173 MS.gene00462:CDS 40.0%
! TGTTGACTATGTACGAAGAG+TGG - chr1.4:4271118-4271137 MS.gene00462:CDS 40.0%
!! CTACCATCAATAGCCTTGTA+AGG + chr1.4:4273222-4273241 None:intergenic 40.0%
!! TGATGAATTGGCCTTGCAAA+AGG - chr1.4:4273444-4273463 MS.gene00462:intron 40.0%
!!! GGAAGGGTCCCATAATTTTT+TGG - chr1.4:4272093-4272112 MS.gene00462:CDS 40.0%
AAATTCATGGGGTGGTAGCT+GGG - chr1.4:4273920-4273939 MS.gene00462:CDS 45.0%
ACACCCTGACAAGAGACAAA+TGG + chr1.4:4273810-4273829 None:intergenic 45.0%
AGGAGATGATCGACATGTCT+AGG + chr1.4:4271047-4271066 None:intergenic 45.0%
AGTATCATCACCACAGCTGA+TGG - chr1.4:4272424-4272443 MS.gene00462:intron 45.0%
ATGGTAGATGTGACCAGAAC+AGG - chr1.4:4273235-4273254 MS.gene00462:intron 45.0%
CACAGACAGGATACTCATGA+GGG + chr1.4:4274232-4274251 None:intergenic 45.0%
CCAAGTAACTAACCCTGTTC+TGG + chr1.4:4273251-4273270 None:intergenic 45.0%
CGTAGGATGAAGAAATTGGG+TGG - chr1.4:4271336-4271355 MS.gene00462:CDS 45.0%
CTAAAACTCCCCGCATAAGT+AGG - chr1.4:4272065-4272084 MS.gene00462:CDS 45.0%
CTCAGTTAATCATGGGGTGA+AGG + chr1.4:4272898-4272917 None:intergenic 45.0%
GGAGATGATCGACATGTCTA+GGG + chr1.4:4271046-4271065 None:intergenic 45.0%
GGCTAGTGAAACTGTGAAAG+TGG + chr1.4:4271025-4271044 None:intergenic 45.0%
GGTGTATTTACCGGTAGATG+TGG - chr1.4:4273825-4273844 MS.gene00462:CDS 45.0%
GTCAAAGACCAAGCAAGTTG+TGG - chr1.4:4271444-4271463 MS.gene00462:intron 45.0%
TACAACGAAGGATGCAATGG+TGG - chr1.4:4273132-4273151 MS.gene00462:intron 45.0%
TAGTAGCTGTCACAGACAGA+TGG + chr1.4:4274092-4274111 None:intergenic 45.0%
TCACAGACAGGATACTCATG+AGG + chr1.4:4274233-4274252 None:intergenic 45.0%
TGGTAGATGTGACCAGAACA+GGG - chr1.4:4273236-4273255 MS.gene00462:intron 45.0%
TTGATAGGTGAAGGTGGTGA+TGG + chr1.4:4274065-4274084 None:intergenic 45.0%
! AATCTAGCTTCAAGCAGAGC+AGG - chr1.4:4273972-4273991 MS.gene00462:CDS 45.0%
! AGTGCTTACCACAACTTGCT+TGG + chr1.4:4271455-4271474 None:intergenic 45.0%
! GGAAGATCTTGCTTTGAGTG+GGG - chr1.4:4274155-4274174 MS.gene00462:CDS 45.0%
! GTATTGTATGTGGCCTTACC+CGG - chr1.4:4272149-4272168 MS.gene00462:CDS 45.0%
! GTTTGCAACAGCCTTTTGCA+AGG + chr1.4:4273458-4273477 None:intergenic 45.0%
! TCTGTTGATTGGAGGAAGGA+AGG - chr1.4:4271408-4271427 MS.gene00462:CDS 45.0%
! TGCCTGAATCTGTTGATTGG+AGG - chr1.4:4271400-4271419 MS.gene00462:CDS 45.0%
!! AGGATGCAATGGTGGACTTA+TGG - chr1.4:4273140-4273159 MS.gene00462:intron 45.0%
!! CCAGAACAGGGTTAGTTACT+TGG - chr1.4:4273248-4273267 MS.gene00462:intron 45.0%
!! GCTTGTTGATTTGTCTGGGT+GGG + chr1.4:4271079-4271098 None:intergenic 45.0%
!! TGCTTGTTGATTTGTCTGGG+TGG + chr1.4:4271080-4271099 None:intergenic 45.0%
!!! TAATTTTAAAATTAAATACA+AGG - chr1.4:4271808-4271827 MS.gene00462:CDS 5.0%
AATCGTTATGCGCCACGTGT+CGG - chr1.4:4271369-4271388 MS.gene00462:CDS 50.0%
AATTCATGGGGTGGTAGCTG+GGG - chr1.4:4273921-4273940 MS.gene00462:CDS 50.0%
AGCTACTACAGCTGTGCTGA+AGG - chr1.4:4274104-4274123 MS.gene00462:CDS 50.0%
ATCCAACAGCTGCAACACCA+TGG + chr1.4:4273863-4273882 None:intergenic 50.0%
CAAAGATCGATCCGAACCGT+AGG - chr1.4:4271319-4271338 MS.gene00462:CDS 50.0%
CAGCAAGTAGCAGACTCAAG+AGG + chr1.4:4274188-4274207 None:intergenic 50.0%
CGCAGTCTTACCCAGTTTCT+TGG - chr1.4:4272125-4272144 MS.gene00462:CDS 50.0%
CGTTAGTTAGATCCGCGAAC+CGG + chr1.4:4271272-4271291 None:intergenic 50.0%
GAAATTCATGGGGTGGTAGC+TGG - chr1.4:4273919-4273938 MS.gene00462:CDS 50.0%
GGCAATTTATCACCGACACG+TGG + chr1.4:4271384-4271403 None:intergenic 50.0%
GTTAGTTAGATCCGCGAACC+GGG + chr1.4:4271271-4271290 None:intergenic 50.0%
TAGCTGTCACAGACAGATGG+TGG + chr1.4:4274089-4274108 None:intergenic 50.0%
TCAAGCAGAGCAGGAAAGTG+TGG - chr1.4:4273981-4274000 MS.gene00462:CDS 50.0%
TCAGCAATCGCAGCTGTTGA+AGG - chr1.4:4273039-4273058 MS.gene00462:intron 50.0%
! CAGACAGATGGTGGCTTGAT+AGG + chr1.4:4274080-4274099 None:intergenic 50.0%
! GATGGTGGCTTGATAGGTGA+AGG + chr1.4:4274074-4274093 None:intergenic 50.0%
! GGTGTTGCAGCTGTTGGATA+CGG - chr1.4:4273864-4273883 MS.gene00462:CDS 50.0%
! TAGTGCCGTTCCACATCTAC+CGG + chr1.4:4273838-4273857 None:intergenic 50.0%
!! AGGAAGGAAGGTGCTGTTGT+TGG - chr1.4:4271420-4271439 MS.gene00462:intron 50.0%
!! TGGTGGCATTGACTCTGAAG+AGG - chr1.4:4273191-4273210 MS.gene00462:intron 50.0%
ACCGCCTCCTTCAACAGCAA+CGG + chr1.4:4273491-4273510 None:intergenic 55.0%
CCTTCAACAGCAACGGCGAT+AGG + chr1.4:4273484-4273503 None:intergenic 55.0%
CTTCCCGGACCCTACTTATG+CGG + chr1.4:4272078-4272097 None:intergenic 55.0%
GCATAAGTAGGGTCCGGGAA+GGG - chr1.4:4272077-4272096 MS.gene00462:CDS 55.0%
GGTGGCTTGATAGGTGAAGG+TGG + chr1.4:4274071-4274090 None:intergenic 55.0%
TGCTGTTGAAGGAGGCGGTA+GGG - chr1.4:4273492-4273511 MS.gene00462:intron 55.0%
TGTGGAACGGCACTAGACCA+TGG - chr1.4:4273843-4273862 MS.gene00462:CDS 55.0%
TTCCCGGACCCTACTTATGC+GGG + chr1.4:4272077-4272096 None:intergenic 55.0%
! ATCGCCGTTGCTGTTGAAGG+AGG - chr1.4:4273484-4273503 MS.gene00462:intron 55.0%
! ATTAGGGTTGACCCGGTTCG+CGG - chr1.4:4271257-4271276 MS.gene00462:CDS 55.0%
! CCTATCGCCGTTGCTGTTGA+AGG - chr1.4:4273481-4273500 MS.gene00462:intron 55.0%
! TTGCTGTTGAAGGAGGCGGT+AGG - chr1.4:4273491-4273510 MS.gene00462:intron 55.0%
!! GACCATGGTGTTGCAGCTGT+TGG - chr1.4:4273858-4273877 MS.gene00462:CDS 55.0%
!! GTCTGGGTGGGTTTTGTCGT+AGG + chr1.4:4271067-4271086 None:intergenic 55.0%
!! GTGAAGGTGGTGATGGTCCA+GGG + chr1.4:4274058-4274077 None:intergenic 55.0%
!! GTGGTGATGGTCCAGGGTTA+GGG + chr1.4:4274052-4274071 None:intergenic 55.0%
!! TGGTGATGGTCCAGGGTTAG+GGG + chr1.4:4274051-4274070 None:intergenic 55.0%
CAACAGCAACGGCGATAGGC+TGG + chr1.4:4273480-4273499 None:intergenic 60.0%
CCAGCACGAGTGTCACAGAC+AGG + chr1.4:4274245-4274264 None:intergenic 60.0%
CCTGTCTGTGACACTCGTGC+TGG - chr1.4:4274242-4274261 MS.gene00462:CDS 60.0%
CGCATAAGTAGGGTCCGGGA+AGG - chr1.4:4272076-4272095 MS.gene00462:CDS 60.0%
CTCCCCGCATAAGTAGGGTC+CGG - chr1.4:4272071-4272090 MS.gene00462:CDS 60.0%
GGTGGTAGCTGGGGTGAACA+AGG - chr1.4:4273930-4273949 MS.gene00462:CDS 60.0%
GTGATGGTCCAGGGTTAGGG+GGG + chr1.4:4274049-4274068 None:intergenic 60.0%
TCCCCGCATAAGTAGGGTCC+GGG - chr1.4:4272072-4272091 MS.gene00462:CDS 60.0%
TCCCGGACCCTACTTATGCG+GGG + chr1.4:4272076-4272095 None:intergenic 60.0%
! GCCGTTGCTGTTGAAGGAGG+CGG - chr1.4:4273487-4273506 MS.gene00462:intron 60.0%
! GGTGATGGTCCAGGGTTAGG+GGG + chr1.4:4274050-4274069 None:intergenic 60.0%
!! GGTGAAGGTGGTGATGGTCC+AGG + chr1.4:4274059-4274078 None:intergenic 60.0%
!! GGTGGTGATGGTCCAGGGTT+AGG + chr1.4:4274053-4274072 None:intergenic 60.0%
!! TGCGGGGAGTTTTAGTGCGC+AGG + chr1.4:4272060-4272079 None:intergenic 60.0%
GGACAGAACCCCCCTAACCC+TGG - chr1.4:4274038-4274057 MS.gene00462:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 4270982 4274277 4270982 ID=MS.gene00462
chr1.4 mRNA 4270982 4274277 4270982 ID=MS.gene00462.t1;Parent=MS.gene00462
chr1.4 exon 4273794 4274277 4273794 ID=MS.gene00462.t1.exon1;Parent=MS.gene00462.t1
chr1.4 CDS 4273794 4274277 4273794 ID=cds.MS.gene00462.t1;Parent=MS.gene00462.t1
chr1.4 exon 4272002 4272237 4272002 ID=MS.gene00462.t1.exon2;Parent=MS.gene00462.t1
chr1.4 CDS 4272002 4272237 4272002 ID=cds.MS.gene00462.t1;Parent=MS.gene00462.t1
chr1.4 exon 4271725 4271865 4271725 ID=MS.gene00462.t1.exon3;Parent=MS.gene00462.t1
chr1.4 CDS 4271725 4271865 4271725 ID=cds.MS.gene00462.t1;Parent=MS.gene00462.t1
chr1.4 exon 4270982 4271434 4270982 ID=MS.gene00462.t1.exon4;Parent=MS.gene00462.t1
chr1.4 CDS 4270982 4271434 4270982 ID=cds.MS.gene00462.t1;Parent=MS.gene00462.t1
Gene Sequence

>MS.gene00462

ATGGATTCAGCGATGAAACTCATGATCGTTCTCATCTTAGCCACTTTCACAGTTTCACTAGCCCTAGACATGTCGATCATCTCCTACGACAAAACCCACCCAGACAAATCAACAAGCAAAAGAACCAACGAAGAAGTGTTGACTATGTACGAAGAGTGGTTAGTGAAACATGGAAAATCCTACAACGGTTTAGGTGAGAAAGACAAAAGGTTCGAGATCTTCAAAGACAACCTTAAATTCATTGATGAACATAACGGTTTGAATTCGACATATAGATTAGGGTTGACCCGGTTCGCGGATCTAACTAACGAAGAGTATAGAAGCAAGTTTTTGGGAACAAAGATCGATCCGAACCGTAGGATGAAGAAATTGGGTGGTGTAAGGAGCAATCGTTATGCGCCACGTGTCGGTGATAAATTGCCTGAATCTGTTGATTGGAGGAAGGAAGGTGCTGTTGTTGGAGTCAAAGACCAAGCAAGTTGTGGTAAGCACTTCGATCACTTATCTTAAACACTTTCGATGTTTTTTACTTTTTTTTTCTTTTTCTTTTAAATAAAAAATAAATAAAGCTAAATTCTTTTCTTTGTTTCTAATCATTCTTTGATTTTTTTCTCAAAAAAAAAAATCATTCTTTGATTTTAAATTAATAATTAATAATATCTACTGTCTGTTACTATTGACTTATTGTGAATATTATTTTGTTTTTAAATTTTATGATCTTATCTTTGATTTACTGCCATCCATGTGTAACAATATCTGGGAAAAAATATTTCAGTGTTAATGATGTACTTTAATTTGTGGCTGAAAAATATTGATTTTATTTATTTAATTTTAAAATTAAATACAAGGCTTTTAATTTTAGATTTTTCTTATTTTCTTGGATATGTTATTAGGATTTCATGATTCTTATTTGTGATCTGACTATGATATGAATCAAAGTATCAACTCTTGATCCTGATAATTCATCTGGATTTCCTTATTTGCTTTGGGATTTCATGTTTTATAGTGAAATTTACAAAAATAAATAAATAAATTGAAGAAGAAACTTACAAAATTTTAATTCATAAAAGGGCAACCTGCGCACTAAAACTCCCCGCATAAGTAGGGTCCGGGAAGGGTCCCATAATTTTTTGGTGTATTGTACGCAGTCTTACCCAGTTTCTTGGTGTATTGTATGTGGCCTTACCCGGTGCACAAAATTTTAAATCATACACACTGATAATCAATTGTCCTCCTAACTCTCAGATCATTAAAATTGTTTGACTTTAATTATACATAGACCATACGATTGATTTTGATTTGCTGATAGTGTTAAACTAAAAACAATTCTTTGGATTTCGTTTGCAGTATGGATTCCTTTTTTTTTTTTGTATTACTTAATACTTAAGTTCTTTGTTTTGTAGTCATATAAGGTCTGTTTGGAAAGTAAATACAGTATAAAGAGTATCATCACCACAGCTGATGGATATCTTACACCTAAAATCACCAGAATTCTTGGTTTGCATTTGTTTGTTCAATATGGGTTTAATTGATATGCACTTTTACCTATCAATCAATCATAACATGGCTCGAAATATACGCCTTTAATCATGTCTACCTTAAAAGTCATACGAAATATATATTTTGTGACTGTTTAACTACCTTAAAAGTTTTACGACGACGGTGCATCAAAATTAATCTCTTTGAATATTCTCAAAATTGTTAAGAGAACACGTAGATAATCATGTAACATGGTTTGTGCTTGTCAATCAGACTCAGATTCTTCAGCTCCCAAGAGTACTCAATATATCAATCATTGGTGTAATAATATGGCTCTATCTTACCATAATATAATGCTTTTTTGTTTGTCAATAAATAATGAATATCCTTTATTTCTTGAGCAGAAGTATTAATCACTCTTCTAATGAAAAAGTCCTTCACCCCATGATTAACTGAGTTTGATTAAGGAACATGATAGTTTTTGGCTTCTTTTAATTGTATTCATACAATTTAATTTGTCCACAAAAAATTGCTAATATTATCTTCTTTTTTTCAATGAAGGGAGTTGTTGGGCATTCTCAGCAATCGCAGCTGTTGAAGGAATAAATAAGATTGTGACAGGAGATCTAATTTCACTATCAGAACAAGAACTGGTGGACTGTGATACATCATACAACGAAGGATGCAATGGTGGACTTATGGATTACGCCTTTGAGTTCATCATCAGTAATGGTGGCATTGACTCTGAAGAGGATTACCCTTACAAGGCTATTGATGGTAGATGTGACCAGAACAGGGTTAGTTACTTGGTCATTGATTTTTTAATGACAATAGTAAATGTTTGTGGAAGATAGTTAATCAGTAGGACTAGTTAGTTAACACTCTAATTCTTTCATTGGTTTTAAACTGTTTGGTTATTCTCTTTGTGCGCAGAAAAATGCTAAAGTTGTTACAATTGATGACTATGAAGATGTTCCTGCTTATGATGAATTGGCCTTGCAAAAGGCTGTTGCAAACCAGCCTATCGCCGTTGCTGTTGAAGGAGGCGGTAGGGAATTCCAGTTATATGAATATGTAAGTTTCTAAATTAATTCTACTATGAAACTAGGAATTTTATACTGTGTTGCATTGGGATAACGTCATAATCAATACTGCAAACGTGTATAAATTGTTATAACTATCCTTCACATATTGATTTTGAATAAAAATCATCTGTCTGGCAAGTGGTTTTGCTATAACTATCCTTCACATTATCTTCAATAGCCTGTTAATTTATTTTTTTTGACTTTGCTTCACAAGATTGGTACATACTGTTATATTTCATGAACTTTCTGTTTGAATGTATTCCATTTGTCTCTTGTCAGGGTGTATTTACCGGTAGATGTGGAACGGCACTAGACCATGGTGTTGCAGCTGTTGGATACGGTACAGAAAATGGGAAAGATTATTGGATTGTGAGAAATTCATGGGGTGGTAGCTGGGGTGAACAAGGTTACATCAGACTCGAACGTAATCTAGCTTCAAGCAGAGCAGGAAAGTGTGGAATTGCGATCGAGCCATCTTATCCAATTAAGAATGGACAGAACCCCCCTAACCCTGGACCATCACCACCTTCACCTATCAAGCCACCATCTGTCTGTGACAGCTACTACAGCTGTGCTGAAGGCTCTACTTGTTGCTGTATTTACGAGTATGGAAGATCTTGCTTTGAGTGGGGATGCTGTCCTCTTGAGTCTGCTACTTGCTGTGATGATCACTACAGTTGCTGCCCTCATGAGTATCCTGTCTGTGACACTCGTGCTGGACTTTGTCTTAAG
Protein sequence

>MS.gene00462.t1

MDSAMKLMIVLILATFTVSLALDMSIISYDKTHPDKSTSKRTNEEVLTMYEEWLVKHGKSYNGLGEKDKRFEIFKDNLKFIDEHNGLNSTYRLGLTRFADLTNEEYRSKFLGTKIDPNRRMKKLGGVRSNRYAPRVGDKLPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRCDQNRKNAKVVTIDDYEDVPAYDELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIVRNSWGGSWGEQGYIRLERNLASSRAGKCGIAIEPSYPIKNGQNPPNPGPSPPSPIKPPSVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESATCCDDHYSCCPHEYPVCDTRAGLCLK