AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00463

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00463.t1 MTR_1g018840 97.655 469 11 0 1 469 6 474 0.0 959
MS.gene00463.t1 MTR_1g018840 97.489 438 11 0 1 438 6 443 0.0 892
MS.gene00463.t1 MTR_3g102220 72.043 465 127 2 7 469 11 474 0.0 687
MS.gene00463.t1 MTR_1g107550 67.972 434 132 3 22 454 26 453 0.0 598
MS.gene00463.t1 MTR_4g094918 64.543 361 114 1 3 363 2 348 6.68e-177 500
MS.gene00463.t1 MTR_3g112430 51.020 392 181 6 49 438 35 417 6.68e-132 389
MS.gene00463.t1 MTR_3g116080 52.339 342 151 4 16 357 19 348 7.97e-127 372
MS.gene00463.t1 MTR_3g075390 50.411 365 171 7 11 373 6 362 3.61e-123 363
MS.gene00463.t1 MTR_4g104370 49.862 363 172 7 13 373 6 360 1.03e-117 349
MS.gene00463.t1 MTR_2g016180 44.787 422 208 8 42 448 43 454 4.05e-117 352
MS.gene00463.t1 MTR_2g016270 44.286 420 198 8 42 448 43 439 7.46e-115 346
MS.gene00463.t1 MTR_4g079470 51.227 326 142 7 37 355 28 343 2.31e-112 335
MS.gene00463.t1 MTR_4g079770 53.035 313 130 7 50 355 41 343 4.63e-112 334
MS.gene00463.t1 MTR_5g022560 48.864 352 151 8 16 355 8 342 7.79e-112 334
MS.gene00463.t1 MTR_4g047610 50.462 325 145 6 38 355 26 341 1.50e-110 330
MS.gene00463.t1 MTR_4g079800 52.396 313 132 7 50 355 41 343 1.62e-110 330
MS.gene00463.t1 MTR_2g075830 48.980 343 153 7 19 355 13 339 1.13e-109 328
MS.gene00463.t1 MTR_4g080730 52.597 308 135 6 50 355 40 338 3.91e-108 324
MS.gene00463.t1 MTR_4g080700 51.948 308 137 6 50 355 40 338 9.58e-107 320
MS.gene00463.t1 MTR_4g079440 45.559 349 179 6 10 356 5 344 2.46e-106 320
MS.gene00463.t1 MTR_4g107930 50.467 321 140 8 45 355 32 343 4.87e-106 319
MS.gene00463.t1 MTR_2g090890 46.307 352 166 7 14 358 3 338 5.42e-106 318
MS.gene00463.t1 MTR_4g080360 47.664 321 161 6 39 356 25 341 6.38e-100 303
MS.gene00463.t1 MTR_2g083930 45.342 322 172 4 38 356 23 343 9.43e-98 298
MS.gene00463.t1 MTR_7g078600 46.326 313 149 10 50 358 42 339 6.43e-86 267
MS.gene00463.t1 MTR_7g079230 43.077 325 166 8 37 355 23 334 5.73e-85 265
MS.gene00463.t1 MTR_2g015850 47.797 295 145 7 64 356 76 363 1.33e-80 254
MS.gene00463.t1 MTR_4g065077 40.872 367 183 14 5 357 11 357 7.52e-76 242
MS.gene00463.t1 MTR_2g015920 37.228 368 207 12 9 362 8 365 9.25e-71 229
MS.gene00463.t1 MTR_2g015930 38.950 362 195 13 11 357 9 359 3.42e-69 224
MS.gene00463.t1 MTR_4g125300 38.227 361 190 12 11 356 6 348 9.65e-67 218
MS.gene00463.t1 MTR_2g067900 42.902 317 165 11 50 356 16 326 2.59e-66 216
MS.gene00463.t1 MTR_8g086470 37.741 363 184 13 5 357 27 357 6.98e-66 216
MS.gene00463.t1 MTR_4g071060 38.983 354 168 12 13 336 6 341 3.74e-63 209
MS.gene00463.t1 MTR_1g023210 41.611 298 148 8 56 336 57 345 4.63e-63 209
MS.gene00463.t1 MTR_2g067560 39.255 349 186 15 48 380 14 352 1.34e-54 196
MS.gene00463.t1 MTR_1g094135 33.962 371 201 10 4 348 6 358 1.50e-54 187
MS.gene00463.t1 MTR_2g064475 37.101 345 169 14 48 354 14 348 3.31e-54 185
MS.gene00463.t1 MTR_1g023810 45.249 221 111 7 138 354 47 261 4.85e-52 177
MS.gene00463.t1 MTR_2g066980 32.861 353 183 12 19 355 3 317 1.44e-50 175
MS.gene00463.t1 MTR_7g078630 37.162 296 134 8 28 311 690 945 1.69e-47 176
MS.gene00463.t1 MTR_4g072790 35.714 294 146 8 50 336 48 305 9.23e-46 162
MS.gene00463.t1 MTR_2g016380 40.553 217 117 4 240 446 18 232 2.58e-45 159
MS.gene00463.t1 MTR_4g072340 32.551 341 179 10 7 336 9 309 6.55e-44 157
MS.gene00463.t1 MTR_4g073660 34.459 296 151 7 50 336 47 308 1.56e-43 156
MS.gene00463.t1 MTR_1g025450 43.103 174 77 5 138 308 53 207 1.06e-32 130
MS.gene00463.t1 MTR_7g078580 50.476 105 49 3 258 361 4 106 6.47e-29 110
MS.gene00463.t1 MTR_2g015840 39.259 135 78 2 42 175 25 156 1.63e-27 111
MS.gene00463.t1 MTR_2g015840 44.954 109 57 3 248 355 164 270 1.36e-24 103
MS.gene00463.t1 MTR_7g079130 50.538 93 44 2 265 356 6 97 1.02e-24 98.2
MS.gene00463.t1 MTR_3g029560 33.962 212 111 8 151 356 49 237 2.67e-21 93.2
MS.gene00463.t1 MTR_7g078310 47.917 96 47 2 140 232 152 247 1.30e-20 91.7
MS.gene00463.t1 MTR_7g111060 27.758 281 158 12 97 348 66 330 7.07e-20 91.3
MS.gene00463.t1 MTR_2g047960 48.101 79 41 0 149 227 7 85 1.84e-18 80.9
MS.gene00463.t1 MTR_7g111050 26.715 277 166 11 97 348 63 327 5.09e-17 82.8
MS.gene00463.t1 MTR_8g087510 33.588 131 79 7 181 308 23 148 4.23e-12 65.1
MS.gene00463.t1 MTR_7g079260 42.466 73 24 2 282 353 7 62 7.43e-11 59.7
MS.gene00463.t1 MTR_1g009480 27.451 102 66 1 5 106 1 94 7.88e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00463.t1 AT1G47128 71.739 460 120 4 8 464 8 460 0.0 656
MS.gene00463.t1 AT5G43060 71.429 455 119 5 9 459 9 456 0.0 640
MS.gene00463.t1 AT3G19390 59.465 449 161 8 9 454 8 438 0.0 530
MS.gene00463.t1 AT4G36880 62.195 328 118 2 41 362 40 367 6.71e-151 434
MS.gene00463.t1 AT1G09850 51.551 419 179 7 44 455 26 427 8.81e-145 421
MS.gene00463.t1 AT3G19400 59.133 323 122 6 40 358 34 350 2.22e-136 397
MS.gene00463.t1 AT4G23520 55.028 358 142 8 5 358 9 351 1.54e-135 395
MS.gene00463.t1 AT4G11310 51.524 361 165 6 1 358 1 354 7.38e-127 373
MS.gene00463.t1 AT4G11320 51.801 361 164 6 3 358 6 361 1.40e-125 370
MS.gene00463.t1 AT4G11320 51.801 361 164 6 3 358 6 361 1.40e-125 370
MS.gene00463.t1 AT3G48340 51.831 355 164 5 12 365 4 352 2.81e-124 366
MS.gene00463.t1 AT1G20850 54.167 336 142 5 23 357 30 354 2.09e-122 361
MS.gene00463.t1 AT4G35350 50.142 351 164 3 7 357 14 353 4.24e-121 358
MS.gene00463.t1 AT5G50260 55.346 318 136 5 49 364 37 350 2.42e-118 351
MS.gene00463.t1 AT1G06260 47.714 350 164 6 11 357 10 343 4.54e-109 327
MS.gene00463.t1 AT3G48350 47.790 362 172 7 5 362 1 349 3.25e-108 325
MS.gene00463.t1 AT5G45890 45.042 353 173 6 7 355 9 344 5.13e-105 317
MS.gene00463.t1 AT3G43960 51.327 339 150 11 37 368 28 358 1.74e-102 311
MS.gene00463.t1 AT3G19400 58.964 251 94 5 40 286 34 279 8.09e-101 303
MS.gene00463.t1 AT2G34080 42.033 364 183 9 1 356 1 344 8.91e-91 280
MS.gene00463.t1 AT2G27420 42.936 361 183 10 5 356 1 347 2.22e-90 279
MS.gene00463.t1 AT3G49340 46.815 314 152 9 50 356 35 340 4.27e-90 278
MS.gene00463.t1 AT4G35350 47.535 284 138 3 7 290 14 286 1.16e-88 272
MS.gene00463.t1 AT1G29080 41.905 315 175 7 46 356 35 345 1.86e-81 256
MS.gene00463.t1 AT1G29090 40.000 365 179 11 9 356 13 354 3.29e-81 256
MS.gene00463.t1 AT3G48350 44.097 288 148 5 5 290 1 277 1.99e-73 233
MS.gene00463.t1 AT1G29110 35.673 342 202 6 19 356 6 333 1.33e-69 225
MS.gene00463.t1 AT5G60360 38.000 350 180 12 27 356 24 356 1.77e-65 215
MS.gene00463.t1 AT3G45310 36.000 375 202 12 1 356 1 356 1.82e-64 212
MS.gene00463.t1 AT4G39090 39.748 317 148 7 42 334 46 343 7.77e-64 211
MS.gene00463.t1 AT5G60360 37.429 350 181 12 27 356 24 355 3.53e-63 209
MS.gene00463.t1 AT5G60360 37.681 345 184 12 27 352 24 356 1.04e-62 208
MS.gene00463.t1 AT2G21430 36.212 359 185 10 1 334 1 340 1.17e-62 207
MS.gene00463.t1 AT3G45310 35.544 377 200 13 1 356 1 355 7.17e-62 206
MS.gene00463.t1 AT4G16190 35.574 357 195 11 6 336 3 350 3.71e-57 194
MS.gene00463.t1 AT3G54940 33.053 357 196 10 6 336 8 347 2.38e-51 178
MS.gene00463.t1 AT3G54940 32.042 284 161 7 6 272 8 276 7.44e-39 142
MS.gene00463.t1 AT4G01610 27.491 291 172 12 97 361 66 343 1.84e-20 93.2
MS.gene00463.t1 AT1G02305 27.698 278 162 11 97 348 69 333 7.37e-20 91.3
MS.gene00463.t1 AT4G01610 26.804 291 174 12 97 361 66 343 1.18e-18 87.8
MS.gene00463.t1 AT1G02300 28.095 210 122 8 161 348 148 350 4.49e-13 70.9

Find 129 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATGGGAAAGATTAT+TGG 0.227075 1.3:-4478203 MS.gene00463:CDS
GAACCGTAGGATGAAGAAAT+TGG 0.244550 1.3:-4480760 MS.gene00463:CDS
TATGTTCATCAATGAATTTA+AGG 0.255434 1.3:+4480880 None:intergenic
GTTGGATACGGTACAGAAAA+TGG 0.255932 1.3:-4478216 MS.gene00463:CDS
GGTGGTGATGGTCCAGGGTT+AGG 0.264790 1.3:+4478042 None:intergenic
GGGTTCTGTCCATTCTTAAT+TGG 0.270191 1.3:+4478066 None:intergenic
TTCCTCCAATCAACAGATTC+AGG 0.289567 1.3:+4480690 None:intergenic
GCCAAGCCTCACTGGGCCTT+TGG 0.294833 1.3:-4476604 MS.gene00463:CDS
TGTTGCTGTATTTACGAGTA+TGG 0.314590 1.3:-4477958 MS.gene00463:CDS
TTTCTCACCTAAACCGTTGT+AGG 0.323971 1.3:+4480932 None:intergenic
AACTTACATATTCATATAAC+TGG 0.326706 1.3:+4478573 None:intergenic
AGGATGCAATGGTGGACTTA+TGG 0.331252 1.3:-4478952 MS.gene00463:CDS
TGAATTCGACATATAGATTA+GGG 0.336748 1.3:-4480851 MS.gene00463:CDS
GTGGTGATGGTCCAGGGTTA+GGG 0.341154 1.3:+4478043 None:intergenic
GGAAAATCCTACAACGGTTT+AGG 0.345309 1.3:-4480939 MS.gene00463:CDS
ATGGTAGATGTGACCAGAAC+AGG 0.354867 1.3:-4478857 MS.gene00463:CDS
GAAATTCATGGGGTGGTAGC+TGG 0.356281 1.3:-4478173 MS.gene00463:CDS
TTGAATTCGACATATAGATT+AGG 0.369544 1.3:-4480852 MS.gene00463:CDS
CGTTAGTTAGATCCGCGAAC+CGG 0.375000 1.3:+4480823 None:intergenic
CAGACAGATGGTGGCTTGAT+AGG 0.385214 1.3:+4478015 None:intergenic
GCACTCCTGCCAAGCCTCAC+TGG 0.389486 1.3:-4476612 MS.gene00463:CDS
TGTTCCTGCTTATGATGAAT+TGG 0.389594 1.3:-4478660 MS.gene00463:CDS
TCTTGTCAGGGTGTATTTAC+CGG 0.390275 1.3:-4478276 MS.gene00463:intron
CCTATCGCCGTTGCTGTTGA+AGG 0.401421 1.3:-4478611 MS.gene00463:CDS
ATTGGATTGTGAGAAATTCA+TGG 0.410060 1.3:-4478185 MS.gene00463:CDS
CCAAGTAACTAACCCTGTTC+TGG 0.415431 1.3:+4478844 None:intergenic
TACCCTTACAAGGCTATTGA+TGG 0.421160 1.3:-4478876 MS.gene00463:CDS
CTACCATCAATAGCCTTGTA+AGG 0.422671 1.3:+4478873 None:intergenic
AATTGCCTGAATCTGTTGAT+TGG 0.426885 1.3:-4480695 MS.gene00463:CDS
AAGGCCCAGTGAGGCTTGGC+AGG 0.429824 1.3:+4476607 None:intergenic
CACTCCTGCCAAGCCTCACT+GGG 0.436398 1.3:-4476611 MS.gene00463:CDS
CCATTCTTAATTGGATAAGA+TGG 0.437304 1.3:+4478075 None:intergenic
AAATTCATGGGGTGGTAGCT+GGG 0.440858 1.3:-4478172 MS.gene00463:CDS
TCAGCAATCGCAGCTGTTGA+AGG 0.443055 1.3:-4479053 MS.gene00463:CDS
TTTCAATGAAGGGAGTTGTT+GGG 0.446061 1.3:-4479081 MS.gene00463:intron
GGTGATGGTCCAGGGTTAGG+GGG 0.448618 1.3:+4478045 None:intergenic
TGGTGATGGTCCAGGGTTAG+GGG 0.449337 1.3:+4478044 None:intergenic
AAGCCTCACTGGGCCTTTGG+TGG 0.453398 1.3:-4476601 MS.gene00463:CDS
GTGATGGTCCAGGGTTAGGG+GGG 0.453507 1.3:+4478046 None:intergenic
CATAGTCAACACTTCTTCGT+TGG 0.459007 1.3:+4480986 None:intergenic
AGGAAGGAAGGTGCTGTTGT+TGG 0.459612 1.3:-4480672 MS.gene00463:CDS
TCGTGCTGGACTTTGTCTTA+AGG 0.462543 1.3:-4477836 MS.gene00463:intron
TTCACTATCAGAACAAGAAC+TGG 0.467996 1.3:-4479000 MS.gene00463:CDS
AATTTCTTCATCCTACGGTT+CGG 0.472057 1.3:+4480762 None:intergenic
GGTGAAGGTGGTGATGGTCC+AGG 0.475487 1.3:+4478036 None:intergenic
TGATGAATTGGCCTTGCAAA+AGG 0.476220 1.3:-4478648 MS.gene00463:CDS
GGAATAAATAAGATTGTGAC+AGG 0.481755 1.3:-4479032 MS.gene00463:CDS
AGGAGATGATCGACATGTCT+AGG 0.481788 1.3:+4481048 None:intergenic
TGAAGAAATTGGGTGGTGTA+AGG 0.482021 1.3:-4480749 MS.gene00463:CDS
CACCCAATTTCTTCATCCTA+CGG 0.483167 1.3:+4480757 None:intergenic
TTGAATGACTTCACTCCCAA+TGG 0.485328 1.3:+4476637 None:intergenic
AGTGCTTACCACAACTTGCT+TGG 0.487527 1.3:+4480640 None:intergenic
GCTTGTTGATTTGTCTGGGT+GGG 0.490913 1.3:+4481016 None:intergenic
CAACAGCAACGGCGATAGGC+TGG 0.494154 1.3:+4478615 None:intergenic
ATTAGGGTTGACCCGGTTCG+CGG 0.494343 1.3:-4480835 MS.gene00463:CDS
CCTTCAACAGCAACGGCGAT+AGG 0.495351 1.3:+4478611 None:intergenic
ACCAAAGGCCCAGTGAGGCT+TGG 0.495376 1.3:+4476603 None:intergenic
AACCGTAGGATGAAGAAATT+GGG 0.496972 1.3:-4480759 MS.gene00463:CDS
TTGGATACGGTACAGAAAAT+GGG 0.502646 1.3:-4478215 MS.gene00463:CDS
CCTGTCTGTGACACTCGTGC+TGG 0.506173 1.3:-4477850 MS.gene00463:CDS
TGGAAGATCTTGCTTTGAGT+GGG 0.510888 1.3:-4477938 MS.gene00463:CDS
CCATCTTATCCAATTAAGAA+TGG 0.514473 1.3:-4478075 MS.gene00463:CDS
TGAATGACTTCACTCCCAAT+GGG 0.526918 1.3:+4476638 None:intergenic
TGCTTGTTGATTTGTCTGGG+TGG 0.529438 1.3:+4481015 None:intergenic
AAATTCATTGATGAACATAA+CGG 0.530458 1.3:-4480876 MS.gene00463:CDS
TCAAGCAGAGCAGGAAAGTG+TGG 0.532736 1.3:-4478111 MS.gene00463:CDS
GGCTAGTGAAACTGTGAAAG+TGG 0.534429 1.3:+4481070 None:intergenic
GTTAGTTAGATCCGCGAACC+GGG 0.534519 1.3:+4480824 None:intergenic
AGCTACTACAGCTGTGCTGA+AGG 0.535391 1.3:-4477988 MS.gene00463:CDS
TTTGAGTTCATCATCAGTAA+TGG 0.537702 1.3:-4478921 MS.gene00463:CDS
AATCTAGCTTCAAGCAGAGC+AGG 0.546872 1.3:-4478120 MS.gene00463:CDS
TTGGATTGTGAGAAATTCAT+GGG 0.548066 1.3:-4478184 MS.gene00463:CDS
GACCATGGTGTTGCAGCTGT+TGG 0.548818 1.3:-4478234 MS.gene00463:CDS
TGCCTGAATCTGTTGATTGG+AGG 0.549740 1.3:-4480692 MS.gene00463:CDS
TGCTGTTGAAGGAGGCGGTA+GGG 0.550318 1.3:-4478600 MS.gene00463:CDS
CAGGGCAAGAATAACCCATT+GGG 0.551019 1.3:-4476652 MS.gene00463:CDS
CATCTTGTTCTTACCACCAA+AGG 0.551674 1.3:+4476588 None:intergenic
GGTGGTAGCTGGGGTGAACA+AGG 0.554597 1.3:-4478162 MS.gene00463:CDS
TTGATAGGTGAAGGTGGTGA+TGG 0.555756 1.3:+4478030 None:intergenic
GCCGTTGCTGTTGAAGGAGG+CGG 0.557434 1.3:-4478605 MS.gene00463:CDS
GGTGTTGCAGCTGTTGGATA+CGG 0.561703 1.3:-4478228 MS.gene00463:CDS
CATATAGATTAGGGTTGACC+CGG 0.561757 1.3:-4480842 MS.gene00463:CDS
ATGGAAGATCTTGCTTTGAG+TGG 0.563923 1.3:-4477939 MS.gene00463:CDS
TAGTGCCGTTCCACATCTAC+CGG 0.569067 1.3:+4478257 None:intergenic
CCAGCACGAGTGTCACAGAC+AGG 0.571924 1.3:+4477850 None:intergenic
TGAAGAGGATTACCCTTACA+AGG 0.572769 1.3:-4478886 MS.gene00463:CDS
AATTCATGGGGTGGTAGCTG+GGG 0.574383 1.3:-4478171 MS.gene00463:CDS
GGAGATGATCGACATGTCTA+GGG 0.575045 1.3:+4481049 None:intergenic
ACAGGGCAAGAATAACCCAT+TGG 0.575173 1.3:-4476653 MS.gene00463:intron
AATCGTTATGCGCCACGTGT+CGG 0.576425 1.3:-4480723 MS.gene00463:CDS
GTTTAGGTGAGAAAGACAAA+AGG 0.579754 1.3:-4480923 MS.gene00463:CDS
GTCAAAGACCAAGCAAGTTG+TGG 0.584862 1.3:-4480648 MS.gene00463:intron
AAGGCCAATTCATCATAAGC+AGG 0.585009 1.3:+4478656 None:intergenic
GGTGTATTTACCGGTAGATG+TGG 0.590389 1.3:-4478267 MS.gene00463:CDS
TACCATCAATAGCCTTGTAA+GGG 0.599159 1.3:+4478874 None:intergenic
GAGTTCATCATCAGTAATGG+TGG 0.599792 1.3:-4478918 MS.gene00463:CDS
ACTATCAGAACAAGAACTGG+TGG 0.602270 1.3:-4478997 MS.gene00463:CDS
GTGAAGGTGGTGATGGTCCA+GGG 0.603987 1.3:+4478037 None:intergenic
ATCGCCGTTGCTGTTGAAGG+AGG 0.609464 1.3:-4478608 MS.gene00463:CDS
TTGCTGTTGAAGGAGGCGGT+AGG 0.609931 1.3:-4478601 MS.gene00463:CDS
GAAGAGTGGTTAGTGAAACA+TGG 0.615801 1.3:-4480960 MS.gene00463:CDS
CAAAGATCGATCCGAACCGT+AGG 0.618623 1.3:-4480773 MS.gene00463:CDS
CAGCAAGTAGCAGACTCAAG+AGG 0.620435 1.3:+4477907 None:intergenic
TAGTAGCTGTCACAGACAGA+TGG 0.620790 1.3:+4478003 None:intergenic
TGGTAGATGTGACCAGAACA+GGG 0.623188 1.3:-4478856 MS.gene00463:intron
TGGTGGCATTGACTCTGAAG+AGG 0.626193 1.3:-4478901 MS.gene00463:CDS
TCATACAACGAAGGATGCAA+TGG 0.627683 1.3:-4478963 MS.gene00463:CDS
TACAACGAAGGATGCAATGG+TGG 0.630463 1.3:-4478960 MS.gene00463:CDS
GGCAATTTATCACCGACACG+TGG 0.638153 1.3:+4480711 None:intergenic
TGTTGACTATGTACGAAGAG+TGG 0.640568 1.3:-4480974 MS.gene00463:CDS
TAGCTGTCACAGACAGATGG+TGG 0.645708 1.3:+4478006 None:intergenic
ATTACTGATGATGAACTCAA+AGG 0.646757 1.3:+4478923 None:intergenic
GGTGGCTTGATAGGTGAAGG+TGG 0.647988 1.3:+4478024 None:intergenic
ACCGCCTCCTTCAACAGCAA+CGG 0.651172 1.3:+4478604 None:intergenic
TCTGTTGATTGGAGGAAGGA+AGG 0.651628 1.3:-4480684 MS.gene00463:CDS
TGAATCTGTTGATTGGAGGA+AGG 0.656666 1.3:-4480688 MS.gene00463:CDS
CACAGACAGGATACTCATGA+GGG 0.662230 1.3:+4477863 None:intergenic
TCACAGACAGGATACTCATG+AGG 0.662392 1.3:+4477862 None:intergenic
ATTTACCGGTAGATGTGGAA+CGG 0.675984 1.3:-4478262 MS.gene00463:CDS
ATCCAACAGCTGCAACACCA+TGG 0.676166 1.3:+4478232 None:intergenic
TGTGGAACGGCACTAGACCA+TGG 0.678729 1.3:-4478249 MS.gene00463:CDS
CGTAGGATGAAGAAATTGGG+TGG 0.680087 1.3:-4480756 MS.gene00463:CDS
GATGGTGGCTTGATAGGTGA+AGG 0.689206 1.3:+4478021 None:intergenic
GGAAGATCTTGCTTTGAGTG+GGG 0.689687 1.3:-4477937 MS.gene00463:CDS
AAACATGGAAAATCCTACAA+CGG 0.700433 1.3:-4480945 MS.gene00463:CDS
ATTGTGAGAAATTCATGGGG+TGG 0.703535 1.3:-4478180 MS.gene00463:CDS
TGTGATACATCATACAACGA+AGG 0.706999 1.3:-4478972 MS.gene00463:CDS
TTACCACCAAAGGCCCAGTG+AGG 0.714697 1.3:+4476598 None:intergenic
TGGATTGTGAGAAATTCATG+GGG 0.737069 1.3:-4478183 MS.gene00463:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAATACAAAAAAAAAAA+AGG + chr1.3:4477934-4477953 None:intergenic 10.0%
!!! ACAAAATTTTAATTCATAAA+AGG - chr1.3:4477624-4477643 MS.gene00463:intron 10.0%
!!! CAAAATTTTAATTCATAAAA+GGG - chr1.3:4477625-4477644 MS.gene00463:intron 10.0%
!!! CCTATAAATTTTTTATAAAA+AGG - chr1.3:4480410-4480429 MS.gene00463:intron 10.0%
!!! CCTTTTTATAAAAAATTTAT+AGG + chr1.3:4480413-4480432 None:intergenic 10.0%
!! AAATTATCCTTATTAACTAT+TGG + chr1.3:4480624-4480643 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr1.3:4480623-4480642 MS.gene00463:intron 15.0%
!! GTCAAAAAAAATAAATTAAC+AGG + chr1.3:4479321-4479340 None:intergenic 15.0%
!! TTAAACTAAAAACAATTCTT+TGG - chr1.3:4477887-4477906 MS.gene00463:CDS 15.0%
!!! AACAAAAAAGCATTATATTA+TGG + chr1.3:4478401-4478420 None:intergenic 15.0%
!!! ATAATATTAGCAATTTTTTG+TGG + chr1.3:4478577-4478596 None:intergenic 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG + chr1.3:4480497-4480516 None:intergenic 15.0%
!!! TCTTAATTTAGTTGTTTTAT+CGG - chr1.3:4480946-4480965 MS.gene00463:CDS 15.0%
!!! TTAGATTTTTCTTATTTTCT+TGG - chr1.3:4477434-4477453 MS.gene00463:intron 15.0%
!! AAACAAAGTCAAATTCTTAT+GGG - chr1.3:4480902-4480921 MS.gene00463:CDS 20.0%
!! AAACTAACCAATAGTTAATA+AGG - chr1.3:4480614-4480633 MS.gene00463:intron 20.0%
!! AAATTCATTGATGAACATAA+CGG - chr1.3:4476810-4476829 MS.gene00463:intron 20.0%
!! AACTTACATATTCATATAAC+TGG + chr1.3:4479116-4479135 None:intergenic 20.0%
!! ACAATACACCAAAAAATTAT+GGG + chr1.3:4477698-4477717 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr1.3:4480537-4480556 None:intergenic 20.0%
!! ATTAATTCTACTATGAAACT+AGG - chr1.3:4479141-4479160 MS.gene00463:intron 20.0%
!! TACAATACACCAAAAAATTA+TGG + chr1.3:4477699-4477718 None:intergenic 20.0%
!! TATGTTCATCAATGAATTTA+AGG + chr1.3:4476809-4476828 None:intergenic 20.0%
!! TATTCAAAATCAATATGTGA+AGG + chr1.3:4479239-4479258 None:intergenic 20.0%
!!! ATCTTCTTTTTTTCAATGAA+GGG - chr1.3:4478595-4478614 MS.gene00463:CDS 20.0%
!!! ATTTTCTTGGATATGTTATT+AGG - chr1.3:4477447-4477466 MS.gene00463:intron 20.0%
!!! CTTAATTTAGTTGTTTTATC+GGG - chr1.3:4480947-4480966 MS.gene00463:CDS 20.0%
!!! TAATGATGTACTTTAATTTG+TGG - chr1.3:4477354-4477373 MS.gene00463:intron 20.0%
!!! TAGTTTTAAAAGTAGTACTT+TGG + chr1.3:4480600-4480619 None:intergenic 20.0%
!!! TATATTTCGTATGACTTTTA+AGG + chr1.3:4478175-4478194 None:intergenic 20.0%
!!! TATCTTCTTTTTTTCAATGA+AGG - chr1.3:4478594-4478613 MS.gene00463:CDS 20.0%
!!! TCCAAGTAATGTTTTAAAAA+TGG + chr1.3:4480106-4480125 None:intergenic 20.0%
!!! TCCATTTTTAAAACATTACT+TGG - chr1.3:4480102-4480121 MS.gene00463:intron 20.0%
! AATCAAACTCAGTTAATCAT+GGG + chr1.3:4478499-4478518 None:intergenic 25.0%
! ACGAATAATAGACATGTTAT+AGG + chr1.3:4480805-4480824 None:intergenic 25.0%
! ATGACAATAGTAAATGTTTG+TGG - chr1.3:4478879-4478898 MS.gene00463:CDS 25.0%
! ATTGATATATTGAGTACTCT+TGG + chr1.3:4478349-4478368 None:intergenic 25.0%
! CAAACAAAGTCAAATTCTTA+TGG - chr1.3:4480901-4480920 MS.gene00463:CDS 25.0%
! CAATCATTGGTGTAATAATA+TGG - chr1.3:4478365-4478384 MS.gene00463:intron 25.0%
! CTTATAATATATCCATAAGC+TGG - chr1.3:4480354-4480373 MS.gene00463:intron 25.0%
! GTAAACAATCCATACAAATT+TGG + chr1.3:4480979-4480998 None:intergenic 25.0%
! GTACTCAATATATCAATCAT+TGG - chr1.3:4478352-4478371 MS.gene00463:intron 25.0%
! TAACACTCTAATTCTTTCAT+TGG - chr1.3:4478931-4478950 MS.gene00463:CDS 25.0%
! TAATCAAACTCAGTTAATCA+TGG + chr1.3:4478500-4478519 None:intergenic 25.0%
! TGAATTCGACATATAGATTA+GGG - chr1.3:4476835-4476854 MS.gene00463:intron 25.0%
! TGATCAAGCACATAAAAATT+TGG + chr1.3:4481014-4481033 None:intergenic 25.0%
! TGATTAACTGAGTTTGATTA+AGG - chr1.3:4478500-4478519 MS.gene00463:intron 25.0%
! TGCATTTGTTTGTTCAATAT+GGG - chr1.3:4478076-4478095 MS.gene00463:CDS 25.0%
! TTATTTAAACTGTTTGATCG+TGG + chr1.3:4480026-4480045 None:intergenic 25.0%
! TTGAATAAAAATCATCTGTC+TGG - chr1.3:4479252-4479271 MS.gene00463:intron 25.0%
! TTGAATTCGACATATAGATT+AGG - chr1.3:4476834-4476853 MS.gene00463:intron 25.0%
! TTGATATATTGAGTACTCTT+GGG + chr1.3:4478348-4478367 None:intergenic 25.0%
! TTGCATTTGTTTGTTCAATA+TGG - chr1.3:4478075-4478094 MS.gene00463:CDS 25.0%
!! AATTTTATACTGTGTTGCAT+TGG - chr1.3:4479164-4479183 MS.gene00463:intron 25.0%
!! ATTTTATACTGTGTTGCATT+GGG - chr1.3:4479165-4479184 MS.gene00463:intron 25.0%
!! CATAAGAATTTGACTTTGTT+TGG + chr1.3:4480903-4480922 None:intergenic 25.0%
!! TTAAGGAACATGATAGTTTT+TGG - chr1.3:4478517-4478536 MS.gene00463:intron 25.0%
!! TTTAAGGTAGACATGATTAA+AGG + chr1.3:4478159-4478178 None:intergenic 25.0%
!!! ATTTTAATGATCTGAGAGTT+AGG + chr1.3:4477812-4477831 None:intergenic 25.0%
!!! CTTTGTTTTGTAGTCATATA+AGG - chr1.3:4477966-4477985 MS.gene00463:CDS 25.0%
!!! TGATTTAAAATTTTGTGCAC+CGG + chr1.3:4477765-4477784 None:intergenic 25.0%
!!! TTCATTGGTTTTAAACTGTT+TGG - chr1.3:4478946-4478965 MS.gene00463:CDS 25.0%
!!! TTTTTGATTGGATTTTCACT+TGG - chr1.3:4479910-4479929 MS.gene00463:intron 25.0%
AAACATGGAAAATCCTACAA+CGG - chr1.3:4476741-4476760 MS.gene00463:intron 30.0%
ACGTAGATAATCATGTAACA+TGG - chr1.3:4478287-4478306 MS.gene00463:intron 30.0%
AGTAAAAGATAAGGTTGACT+TGG + chr1.3:4479881-4479900 None:intergenic 30.0%
ATACAATACACCAAGAAACT+GGG + chr1.3:4477732-4477751 None:intergenic 30.0%
ATCAAACTCAGTTAATCATG+GGG + chr1.3:4478498-4478517 None:intergenic 30.0%
ATCCATGTGTAACAATATCT+GGG - chr1.3:4477314-4477333 MS.gene00463:intron 30.0%
ATGAAATCCCAAAGCAAATA+AGG + chr1.3:4477553-4477572 None:intergenic 30.0%
ATTGGATTGTGAGAAATTCA+TGG - chr1.3:4479501-4479520 MS.gene00463:intron 30.0%
CAGAAAATGGGAAAGATTAT+TGG - chr1.3:4479483-4479502 MS.gene00463:intron 30.0%
CATTACTTGGAACTAATTGT+TGG - chr1.3:4480115-4480134 MS.gene00463:intron 30.0%
CCAATTTGAGCTGTTTAAAA+CGG + chr1.3:4479996-4480015 None:intergenic 30.0%
CCATCTTATCCAATTAAGAA+TGG - chr1.3:4479611-4479630 MS.gene00463:intron 30.0%
CCATGTTATGATTGATTGAT+AGG + chr1.3:4478123-4478142 None:intergenic 30.0%
CCATTCTTAATTGGATAAGA+TGG + chr1.3:4479614-4479633 None:intergenic 30.0%
CCTATCAATCAATCATAACA+TGG - chr1.3:4478120-4478139 MS.gene00463:CDS 30.0%
GCAAATCAAAATCAATCGTA+TGG + chr1.3:4477859-4477878 None:intergenic 30.0%
GGAATAAATAAGATTGTGAC+AGG - chr1.3:4478654-4478673 MS.gene00463:CDS 30.0%
GGGAATTATCCAAATTTGTA+TGG - chr1.3:4480967-4480986 MS.gene00463:CDS 30.0%
TACTTCTGCTCAAGAAATAA+AGG + chr1.3:4478444-4478463 None:intergenic 30.0%
TTGGATTGTGAGAAATTCAT+GGG - chr1.3:4479502-4479521 MS.gene00463:intron 30.0%
! AACCTTATCTTTTACTTGCT+TGG - chr1.3:4479885-4479904 MS.gene00463:intron 30.0%
! AGAGTATAGAAGCAAGTTTT+TGG - chr1.3:4476887-4476906 MS.gene00463:intron 30.0%
! ATTACTGATGATGAACTCAA+AGG + chr1.3:4478766-4478785 None:intergenic 30.0%
! ATTTTTATGTGCTTGATCAC+AGG - chr1.3:4481015-4481034 MS.gene00463:CDS 30.0%
! GAGTATAGAAGCAAGTTTTT+GGG - chr1.3:4476888-4476907 MS.gene00463:intron 30.0%
! TGGATTTCCTTATTTGCTTT+GGG - chr1.3:4477543-4477562 MS.gene00463:intron 30.0%
! TTTGAGTTCATCATCAGTAA+TGG - chr1.3:4478765-4478784 MS.gene00463:intron 30.0%
! TTTTTATGTGCTTGATCACA+GGG - chr1.3:4481016-4481035 MS.gene00463:CDS 30.0%
!! CTAATTGTTGGCTACTATTT+TGG - chr1.3:4480127-4480146 MS.gene00463:intron 30.0%
!! GATTTAAAATTTTGTGCACC+GGG + chr1.3:4477764-4477783 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG + chr1.3:4480705-4480724 None:intergenic 30.0%
!!! TTTTGCTTGTTGATTTGTCT+GGG + chr1.3:4476677-4476696 None:intergenic 30.0%
AAACGTAAAGACTTCAGTCA+TGG - chr1.3:4479965-4479984 MS.gene00463:intron 35.0%
AAAGTCCAAATGAACGCAAA+AGG + chr1.3:4480175-4480194 None:intergenic 35.0%
AACCGTAGGATGAAGAAATT+GGG - chr1.3:4476927-4476946 MS.gene00463:intron 35.0%
AAGTCCAAATGAACGCAAAA+GGG + chr1.3:4480174-4480193 None:intergenic 35.0%
AATTGCCTGAATCTGTTGAT+TGG - chr1.3:4476991-4477010 MS.gene00463:intron 35.0%
AATTTCTTCATCCTACGGTT+CGG + chr1.3:4476927-4476946 None:intergenic 35.0%
AGTCTTTACGTTTCAATGTC+CGG + chr1.3:4479958-4479977 None:intergenic 35.0%
ATTCCATTTGTCTCTTGTCA+GGG - chr1.3:4479398-4479417 MS.gene00463:intron 35.0%
CAAATGCAAACCAAGAATTC+TGG + chr1.3:4478064-4478083 None:intergenic 35.0%
CAATGAACAGACACTATCTA+CGG + chr1.3:4480054-4480073 None:intergenic 35.0%
CAGATATTGTTACACATGGA+TGG + chr1.3:4477315-4477334 None:intergenic 35.0%
CATACAATACACCAAGAAAC+TGG + chr1.3:4477733-4477752 None:intergenic 35.0%
CATCCATGTGTAACAATATC+TGG - chr1.3:4477313-4477332 MS.gene00463:intron 35.0%
CCTAAAATCACCAGAATTCT+TGG - chr1.3:4478051-4478070 MS.gene00463:CDS 35.0%
CTTGATCCTGATAATTCATC+TGG - chr1.3:4477523-4477542 MS.gene00463:intron 35.0%
GGAAATCCAGATGAATTATC+AGG + chr1.3:4477532-4477551 None:intergenic 35.0%
GGCTATTGAAGATAATGTGA+AGG + chr1.3:4479300-4479319 None:intergenic 35.0%
GTAGTCATATAAGGTCTGTT+TGG - chr1.3:4477975-4477994 MS.gene00463:CDS 35.0%
GTGGAAGATAGTTAATCAGT+AGG - chr1.3:4478898-4478917 MS.gene00463:CDS 35.0%
GTTTAGGTGAGAAAGACAAA+AGG - chr1.3:4476763-4476782 MS.gene00463:intron 35.0%
TATTCCATTTGTCTCTTGTC+AGG - chr1.3:4479397-4479416 MS.gene00463:intron 35.0%
TGCCAAGCAAGTAAAAGATA+AGG + chr1.3:4479890-4479909 None:intergenic 35.0%
TGGATTGTGAGAAATTCATG+GGG - chr1.3:4479503-4479522 MS.gene00463:intron 35.0%
TGTGATACATCATACAACGA+AGG - chr1.3:4478714-4478733 MS.gene00463:intron 35.0%
TGTTCCTGCTTATGATGAAT+TGG - chr1.3:4479026-4479045 MS.gene00463:CDS 35.0%
TGTTGCTGTATTTACGAGTA+TGG - chr1.3:4479728-4479747 MS.gene00463:intron 35.0%
TTAATGATCTGAGAGTTAGG+AGG + chr1.3:4477809-4477828 None:intergenic 35.0%
TTCACTATCAGAACAAGAAC+TGG - chr1.3:4478686-4478705 MS.gene00463:CDS 35.0%
TTCCCAGATATTGTTACACA+TGG + chr1.3:4477319-4477338 None:intergenic 35.0%
TTGACTTTGCTTCACAAGAT+TGG - chr1.3:4479336-4479355 MS.gene00463:intron 35.0%
TTGGATACGGTACAGAAAAT+GGG - chr1.3:4479471-4479490 MS.gene00463:intron 35.0%
TTTATCTGCTGAGACTGTTT+GGG - chr1.3:4480762-4480781 MS.gene00463:CDS 35.0%
TTTCAATGAAGGGAGTTGTT+GGG - chr1.3:4478605-4478624 MS.gene00463:CDS 35.0%
TTTGGATTTCGTTTGCAGTA+TGG - chr1.3:4477905-4477924 MS.gene00463:CDS 35.0%
! ACTTGCTTGGCATTTTTGAT+TGG - chr1.3:4479898-4479917 MS.gene00463:intron 35.0%
! CCAAGAATTCTGGTGATTTT+AGG + chr1.3:4478054-4478073 None:intergenic 35.0%
! CCTTAAAAGTTTTACGACGA+CGG - chr1.3:4478216-4478235 MS.gene00463:CDS 35.0%
! CTGGATTTCCTTATTTGCTT+TGG - chr1.3:4477542-4477561 MS.gene00463:intron 35.0%
! GATAATCTGAAACCAGCTTA+TGG + chr1.3:4480369-4480388 None:intergenic 35.0%
! GTTTCTTGGTGTATTGTATG+TGG - chr1.3:4477733-4477752 MS.gene00463:intron 35.0%
! TTTTCAATGAAGGGAGTTGT+TGG - chr1.3:4478604-4478623 MS.gene00463:CDS 35.0%
!! AAGCGACACTCATTTTGAAA+CGG + chr1.3:4480471-4480490 None:intergenic 35.0%
!! CAATGCTTTAAAAGCCAAAC+CGG - chr1.3:4479936-4479955 MS.gene00463:intron 35.0%
!! CCGTTTTAAACAGCTCAAAT+TGG - chr1.3:4479993-4480012 MS.gene00463:intron 35.0%
!! TACCATCAATAGCCTTGTAA+GGG + chr1.3:4478815-4478834 None:intergenic 35.0%
!!! CTTTTGCTTGTTGATTTGTC+TGG + chr1.3:4476678-4476697 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG - chr1.3:4480710-4480729 MS.gene00463:CDS 35.0%
AAAAATTATGGGACCCTTCC+CGG + chr1.3:4477687-4477706 None:intergenic 40.0%
AAAATCATCTGTCTGGCAAG+TGG - chr1.3:4479259-4479278 MS.gene00463:intron 40.0%
AACAGACACTATCTACGGTT+CGG + chr1.3:4480049-4480068 None:intergenic 40.0%
AAGGCCAATTCATCATAAGC+AGG + chr1.3:4479033-4479052 None:intergenic 40.0%
ACTATCAGAACAAGAACTGG+TGG - chr1.3:4478689-4478708 MS.gene00463:CDS 40.0%
ATTGTGAGAAATTCATGGGG+TGG - chr1.3:4479506-4479525 MS.gene00463:intron 40.0%
ATTTACCGGTAGATGTGGAA+CGG - chr1.3:4479424-4479443 MS.gene00463:intron 40.0%
CACCCAATTTCTTCATCCTA+CGG + chr1.3:4476932-4476951 None:intergenic 40.0%
CATAGTCAACACTTCTTCGT+TGG + chr1.3:4476703-4476722 None:intergenic 40.0%
CATATAGATTAGGGTTGACC+CGG - chr1.3:4476844-4476863 MS.gene00463:intron 40.0%
CATCTTGTTCTTACCACCAA+AGG + chr1.3:4481101-4481120 None:intergenic 40.0%
GAACCGTAGGATGAAGAAAT+TGG - chr1.3:4476926-4476945 MS.gene00463:intron 40.0%
GAAGAGTGGTTAGTGAAACA+TGG - chr1.3:4476726-4476745 MS.gene00463:intron 40.0%
GAGTTCATCATCAGTAATGG+TGG - chr1.3:4478768-4478787 MS.gene00463:intron 40.0%
GGAAAATCCTACAACGGTTT+AGG - chr1.3:4476747-4476766 MS.gene00463:intron 40.0%
GGGTTCTGTCCATTCTTAAT+TGG + chr1.3:4479623-4479642 None:intergenic 40.0%
GTTGGATACGGTACAGAAAA+TGG - chr1.3:4479470-4479489 MS.gene00463:intron 40.0%
GTTTATCTGCTGAGACTGTT+TGG - chr1.3:4480761-4480780 MS.gene00463:CDS 40.0%
TAAAACTCCCCGCATAAGTA+GGG - chr1.3:4477660-4477679 MS.gene00463:intron 40.0%
TACCCTTACAAGGCTATTGA+TGG - chr1.3:4478810-4478829 MS.gene00463:intron 40.0%
TCATACAACGAAGGATGCAA+TGG - chr1.3:4478723-4478742 MS.gene00463:intron 40.0%
TCTTGTCAGGGTGTATTTAC+CGG - chr1.3:4479410-4479429 MS.gene00463:intron 40.0%
TGAAGAAATTGGGTGGTGTA+AGG - chr1.3:4476937-4476956 MS.gene00463:intron 40.0%
TGAAGAGGATTACCCTTACA+AGG - chr1.3:4478800-4478819 MS.gene00463:intron 40.0%
TGAATGACTTCACTCCCAAT+GGG + chr1.3:4481051-4481070 None:intergenic 40.0%
TGTAAGATATCCATCAGCTG+TGG + chr1.3:4478031-4478050 None:intergenic 40.0%
TTACGTTTCAATGTCCGGTT+TGG + chr1.3:4479953-4479972 None:intergenic 40.0%
TTCCTCCAATCAACAGATTC+AGG + chr1.3:4476999-4477018 None:intergenic 40.0%
TTGAATGACTTCACTCCCAA+TGG + chr1.3:4481052-4481071 None:intergenic 40.0%
TTTCTCACCTAAACCGTTGT+AGG + chr1.3:4476757-4476776 None:intergenic 40.0%
! AAAATTTTGTGCACCGGGTA+AGG + chr1.3:4477759-4477778 None:intergenic 40.0%
! ATGGAAGATCTTGCTTTGAG+TGG - chr1.3:4479747-4479766 MS.gene00463:intron 40.0%
! ATGTCCCTTTTGCGTTCATT+TGG - chr1.3:4480167-4480186 MS.gene00463:intron 40.0%
! CCGTCGTCGTAAAACTTTTA+AGG + chr1.3:4478219-4478238 None:intergenic 40.0%
! TGAATCTGTTGATTGGAGGA+AGG - chr1.3:4476998-4477017 MS.gene00463:intron 40.0%
! TGGAAGATCTTGCTTTGAGT+GGG - chr1.3:4479748-4479767 MS.gene00463:intron 40.0%
! TGTTGACTATGTACGAAGAG+TGG - chr1.3:4476712-4476731 MS.gene00463:intron 40.0%
!! CTACCATCAATAGCCTTGTA+AGG + chr1.3:4478816-4478835 None:intergenic 40.0%
!! TGATGAATTGGCCTTGCAAA+AGG - chr1.3:4479038-4479057 MS.gene00463:CDS 40.0%
!!! CGACACTCATTTTGAAACAG+AGG - chr1.3:4480709-4480728 MS.gene00463:CDS 40.0%
!!! CGGTTTGGCTTTTAAAGCAT+TGG + chr1.3:4479938-4479957 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr1.3:4480467-4480486 None:intergenic 40.0%
!!! GGAAGGGTCCCATAATTTTT+TGG - chr1.3:4477687-4477706 MS.gene00463:intron 40.0%
AAATTCATGGGGTGGTAGCT+GGG - chr1.3:4479514-4479533 MS.gene00463:intron 45.0%
ACACCCTGACAAGAGACAAA+TGG + chr1.3:4479404-4479423 None:intergenic 45.0%
ACAGGGCAAGAATAACCCAT+TGG - chr1.3:4481033-4481052 MS.gene00463:CDS 45.0%
ACTGGTTGAACTGTCTTGTC+TGG - chr1.3:4480078-4480097 MS.gene00463:intron 45.0%
AGGAGATGATCGACATGTCT+AGG + chr1.3:4476641-4476660 None:intergenic 45.0%
AGTATCATCACCACAGCTGA+TGG - chr1.3:4478018-4478037 MS.gene00463:CDS 45.0%
ATGGTAGATGTGACCAGAAC+AGG - chr1.3:4478829-4478848 MS.gene00463:intron 45.0%
CACAGACAGGATACTCATGA+GGG + chr1.3:4479826-4479845 None:intergenic 45.0%
CAGGGCAAGAATAACCCATT+GGG - chr1.3:4481034-4481053 MS.gene00463:CDS 45.0%
CCAAGTAACTAACCCTGTTC+TGG + chr1.3:4478845-4478864 None:intergenic 45.0%
CGCAAAAGGGACATATCAAC+AGG + chr1.3:4480161-4480180 None:intergenic 45.0%
CGTAGGATGAAGAAATTGGG+TGG - chr1.3:4476930-4476949 MS.gene00463:intron 45.0%
CTAAAACTCCCCGCATAAGT+AGG - chr1.3:4477659-4477678 MS.gene00463:intron 45.0%
CTCAGTTAATCATGGGGTGA+AGG + chr1.3:4478492-4478511 None:intergenic 45.0%
GGAGATGATCGACATGTCTA+GGG + chr1.3:4476640-4476659 None:intergenic 45.0%
GGCTAGTGAAACTGTGAAAG+TGG + chr1.3:4476619-4476638 None:intergenic 45.0%
GGTGTATTTACCGGTAGATG+TGG - chr1.3:4479419-4479438 MS.gene00463:intron 45.0%
GTCAAAGACCAAGCAAGTTG+TGG - chr1.3:4477038-4477057 MS.gene00463:intron 45.0%
GTGTCTGTTCATTGCTCAAC+TGG - chr1.3:4480060-4480079 MS.gene00463:intron 45.0%
TACAACGAAGGATGCAATGG+TGG - chr1.3:4478726-4478745 MS.gene00463:intron 45.0%
TAGTAGCTGTCACAGACAGA+TGG + chr1.3:4479686-4479705 None:intergenic 45.0%
TCACAGACAGGATACTCATG+AGG + chr1.3:4479827-4479846 None:intergenic 45.0%
TCGTGCTGGACTTTGTCTTA+AGG - chr1.3:4479850-4479869 MS.gene00463:intron 45.0%
TGAGACTGTTTGGGAGAACA+TGG - chr1.3:4480771-4480790 MS.gene00463:CDS 45.0%
TGGTAGATGTGACCAGAACA+GGG - chr1.3:4478830-4478849 MS.gene00463:intron 45.0%
TTGATAGGTGAAGGTGGTGA+TGG + chr1.3:4479659-4479678 None:intergenic 45.0%
! AATCTAGCTTCAAGCAGAGC+AGG - chr1.3:4479566-4479585 MS.gene00463:intron 45.0%
! AGTGCTTACCACAACTTGCT+TGG + chr1.3:4477049-4477068 None:intergenic 45.0%
! GGAAGATCTTGCTTTGAGTG+GGG - chr1.3:4479749-4479768 MS.gene00463:intron 45.0%
! GTATTGTATGTGGCCTTACC+CGG - chr1.3:4477743-4477762 MS.gene00463:intron 45.0%
! GTTTGCAACAGCCTTTTGCA+AGG + chr1.3:4479052-4479071 None:intergenic 45.0%
! TCTGTTGATTGGAGGAAGGA+AGG - chr1.3:4477002-4477021 MS.gene00463:intron 45.0%
! TGCCTGAATCTGTTGATTGG+AGG - chr1.3:4476994-4477013 MS.gene00463:intron 45.0%
!! AGGATGCAATGGTGGACTTA+TGG - chr1.3:4478734-4478753 MS.gene00463:intron 45.0%
!! CCAGAACAGGGTTAGTTACT+TGG - chr1.3:4478842-4478861 MS.gene00463:intron 45.0%
!! GCTTGTTGATTTGTCTGGGT+GGG + chr1.3:4476673-4476692 None:intergenic 45.0%
!! TGCTTGTTGATTTGTCTGGG+TGG + chr1.3:4476674-4476693 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr1.3:4480468-4480487 None:intergenic 45.0%
!!! TAATTTTAAAATTAAATACA+AGG - chr1.3:4477402-4477421 MS.gene00463:intron 5.0%
AATCGTTATGCGCCACGTGT+CGG - chr1.3:4476963-4476982 MS.gene00463:intron 50.0%
AATTCATGGGGTGGTAGCTG+GGG - chr1.3:4479515-4479534 MS.gene00463:intron 50.0%
AGCTACTACAGCTGTGCTGA+AGG - chr1.3:4479698-4479717 MS.gene00463:intron 50.0%
ATCCAACAGCTGCAACACCA+TGG + chr1.3:4479457-4479476 None:intergenic 50.0%
CAAAGATCGATCCGAACCGT+AGG - chr1.3:4476913-4476932 MS.gene00463:intron 50.0%
CAGCAAGTAGCAGACTCAAG+AGG + chr1.3:4479782-4479801 None:intergenic 50.0%
CGCAGTCTTACCCAGTTTCT+TGG - chr1.3:4477719-4477738 MS.gene00463:intron 50.0%
CGTTAGTTAGATCCGCGAAC+CGG + chr1.3:4476866-4476885 None:intergenic 50.0%
GAAATTCATGGGGTGGTAGC+TGG - chr1.3:4479513-4479532 MS.gene00463:intron 50.0%
GGCAATTTATCACCGACACG+TGG + chr1.3:4476978-4476997 None:intergenic 50.0%
GTTAGTTAGATCCGCGAACC+GGG + chr1.3:4476865-4476884 None:intergenic 50.0%
TAGCTGTCACAGACAGATGG+TGG + chr1.3:4479683-4479702 None:intergenic 50.0%
TCAAGCAGAGCAGGAAAGTG+TGG - chr1.3:4479575-4479594 MS.gene00463:intron 50.0%
TCAGCAATCGCAGCTGTTGA+AGG - chr1.3:4478633-4478652 MS.gene00463:CDS 50.0%
! CAGACAGATGGTGGCTTGAT+AGG + chr1.3:4479674-4479693 None:intergenic 50.0%
! GATGGTGGCTTGATAGGTGA+AGG + chr1.3:4479668-4479687 None:intergenic 50.0%
! GGTGTTGCAGCTGTTGGATA+CGG - chr1.3:4479458-4479477 MS.gene00463:intron 50.0%
! TAGTGCCGTTCCACATCTAC+CGG + chr1.3:4479432-4479451 None:intergenic 50.0%
!! AGGAAGGAAGGTGCTGTTGT+TGG - chr1.3:4477014-4477033 MS.gene00463:intron 50.0%
!! TGGTGGCATTGACTCTGAAG+AGG - chr1.3:4478785-4478804 MS.gene00463:intron 50.0%
ACCGCCTCCTTCAACAGCAA+CGG + chr1.3:4479085-4479104 None:intergenic 55.0%
CCTTCAACAGCAACGGCGAT+AGG + chr1.3:4479078-4479097 None:intergenic 55.0%
CTTCCCGGACCCTACTTATG+CGG + chr1.3:4477672-4477691 None:intergenic 55.0%
GCATAAGTAGGGTCCGGGAA+GGG - chr1.3:4477671-4477690 MS.gene00463:intron 55.0%
GGTGGCTTGATAGGTGAAGG+TGG + chr1.3:4479665-4479684 None:intergenic 55.0%
TGCTGTTGAAGGAGGCGGTA+GGG - chr1.3:4479086-4479105 MS.gene00463:intron 55.0%
TGTGGAACGGCACTAGACCA+TGG - chr1.3:4479437-4479456 MS.gene00463:intron 55.0%
TTACCACCAAAGGCCCAGTG+AGG + chr1.3:4481091-4481110 None:intergenic 55.0%
TTCCCGGACCCTACTTATGC+GGG + chr1.3:4477671-4477690 None:intergenic 55.0%
! ATCGCCGTTGCTGTTGAAGG+AGG - chr1.3:4479078-4479097 MS.gene00463:intron 55.0%
! ATTAGGGTTGACCCGGTTCG+CGG - chr1.3:4476851-4476870 MS.gene00463:intron 55.0%
! CCTATCGCCGTTGCTGTTGA+AGG - chr1.3:4479075-4479094 MS.gene00463:intron 55.0%
! TTGCTGTTGAAGGAGGCGGT+AGG - chr1.3:4479085-4479104 MS.gene00463:intron 55.0%
!! GACCATGGTGTTGCAGCTGT+TGG - chr1.3:4479452-4479471 MS.gene00463:intron 55.0%
!! GTCTGGGTGGGTTTTGTCGT+AGG + chr1.3:4476661-4476680 None:intergenic 55.0%
!! GTGAAGGTGGTGATGGTCCA+GGG + chr1.3:4479652-4479671 None:intergenic 55.0%
!! GTGGTGATGGTCCAGGGTTA+GGG + chr1.3:4479646-4479665 None:intergenic 55.0%
!! TGGTGATGGTCCAGGGTTAG+GGG + chr1.3:4479645-4479664 None:intergenic 55.0%
AAGCCTCACTGGGCCTTTGG+TGG - chr1.3:4481085-4481104 MS.gene00463:CDS 60.0%
ACCAAAGGCCCAGTGAGGCT+TGG + chr1.3:4481086-4481105 None:intergenic 60.0%
CAACAGCAACGGCGATAGGC+TGG + chr1.3:4479074-4479093 None:intergenic 60.0%
CACTCCTGCCAAGCCTCACT+GGG - chr1.3:4481075-4481094 MS.gene00463:CDS 60.0%
CCAGCACGAGTGTCACAGAC+AGG + chr1.3:4479839-4479858 None:intergenic 60.0%
CCTGTCTGTGACACTCGTGC+TGG - chr1.3:4479836-4479855 MS.gene00463:intron 60.0%
CGCATAAGTAGGGTCCGGGA+AGG - chr1.3:4477670-4477689 MS.gene00463:intron 60.0%
CTCCCCGCATAAGTAGGGTC+CGG - chr1.3:4477665-4477684 MS.gene00463:intron 60.0%
GGTGGTAGCTGGGGTGAACA+AGG - chr1.3:4479524-4479543 MS.gene00463:intron 60.0%
GTGATGGTCCAGGGTTAGGG+GGG + chr1.3:4479643-4479662 None:intergenic 60.0%
TCCCCGCATAAGTAGGGTCC+GGG - chr1.3:4477666-4477685 MS.gene00463:intron 60.0%
TCCCGGACCCTACTTATGCG+GGG + chr1.3:4477670-4477689 None:intergenic 60.0%
! GCCGTTGCTGTTGAAGGAGG+CGG - chr1.3:4479081-4479100 MS.gene00463:intron 60.0%
! GGTGATGGTCCAGGGTTAGG+GGG + chr1.3:4479644-4479663 None:intergenic 60.0%
!! GGTGAAGGTGGTGATGGTCC+AGG + chr1.3:4479653-4479672 None:intergenic 60.0%
!! GGTGGTGATGGTCCAGGGTT+AGG + chr1.3:4479647-4479666 None:intergenic 60.0%
!! TGCGGGGAGTTTTAGTGCGC+AGG + chr1.3:4477654-4477673 None:intergenic 60.0%
AAGGCCCAGTGAGGCTTGGC+AGG + chr1.3:4481082-4481101 None:intergenic 65.0%
GCACTCCTGCCAAGCCTCAC+TGG - chr1.3:4481074-4481093 MS.gene00463:CDS 65.0%
GCCAAGCCTCACTGGGCCTT+TGG - chr1.3:4481082-4481101 MS.gene00463:CDS 65.0%
GGACAGAACCCCCCTAACCC+TGG - chr1.3:4479632-4479651 MS.gene00463:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 4476576 4481132 4476576 ID=MS.gene00463
chr1.3 mRNA 4476576 4481132 4476576 ID=MS.gene00463.t1;Parent=MS.gene00463
chr1.3 exon 4480649 4481132 4480649 ID=MS.gene00463.t1.exon1;Parent=MS.gene00463.t1
chr1.3 CDS 4480649 4481132 4480649 ID=cds.MS.gene00463.t1;Parent=MS.gene00463.t1
chr1.3 exon 4478857 4479092 4478857 ID=MS.gene00463.t1.exon2;Parent=MS.gene00463.t1
chr1.3 CDS 4478857 4479092 4478857 ID=cds.MS.gene00463.t1;Parent=MS.gene00463.t1
chr1.3 exon 4478580 4478720 4478580 ID=MS.gene00463.t1.exon3;Parent=MS.gene00463.t1
chr1.3 CDS 4478580 4478720 4478580 ID=cds.MS.gene00463.t1;Parent=MS.gene00463.t1
chr1.3 exon 4477837 4478289 4477837 ID=MS.gene00463.t1.exon4;Parent=MS.gene00463.t1
chr1.3 CDS 4477837 4478289 4477837 ID=cds.MS.gene00463.t1;Parent=MS.gene00463.t1
chr1.3 exon 4476576 4476671 4476576 ID=MS.gene00463.t1.exon5;Parent=MS.gene00463.t1
chr1.3 CDS 4476576 4476671 4476576 ID=cds.MS.gene00463.t1;Parent=MS.gene00463.t1
Gene Sequence

>MS.gene00463

ATGGATTCAGCGATGAAACTCATGATCGTTCTCATCTTAGCCACTTTCACAGTTTCACTAGCCCTAGACATGTCGATCATCTCCTACGACAAAACCCACCCAGACAAATCAACAAGCAAAAGAACCAACGAAGAAGTGTTGACTATGTACGAAGAGTGGTTAGTGAAACATGGAAAATCCTACAACGGTTTAGGTGAGAAAGACAAAAGGTTCGAGATCTTCAAAGACAACCTTAAATTCATTGATGAACATAACGGTTTGAATTCGACATATAGATTAGGGTTGACCCGGTTCGCGGATCTAACTAACGAAGAGTATAGAAGCAAGTTTTTGGGAACAAAGATCGATCCGAACCGTAGGATGAAGAAATTGGGTGGTGTAAGGAGCAATCGTTATGCGCCACGTGTCGGTGATAAATTGCCTGAATCTGTTGATTGGAGGAAGGAAGGTGCTGTTGTTGGAGTCAAAGACCAAGCAAGTTGTGGTAAGCACTTCGATCACTTATCTTAAACACTTTCGATGTTTTTTACTTTTTTTTTCTTTTTCTTTTAAATAAAAAATAAATAAAGCTAAATTCTTTTCTTTGTTTCTAATCATTCTTTGATTTTTTTCTCAAAAAAAAAAATCATTCTTTGATTTTAAATTAATAATTAATAATATCTACTGTCTGTTACTATTGACTTATTGTGAATATTATTTTGTTTTTAAATTTTATGATCTTATCTTTGATTTACTGCCATCCATGTGTAACAATATCTGGGAAAAAATATTTCAGTGTTAATGATGTACTTTAATTTGTGGCTGAAAAATATTGATTTTATTTATTTAATTTTAAAATTAAATACAAGGCTTTTAATTTTAGATTTTTCTTATTTTCTTGGATATGTTATTAGGATTTCATGATTCTTATTTGTGATCTGACTATGATATGAATCAAAGTATCAACTCTTGATCCTGATAATTCATCTGGATTTCCTTATTTGCTTTGGGATTTCATGTTTTATAGTGAAATTTACAAAAATAAATAAATAAATTGAAGAAGAAACTTACAAAATTTTAATTCATAAAAGGGCAACCTGCGCACTAAAACTCCCCGCATAAGTAGGGTCCGGGAAGGGTCCCATAATTTTTTGGTGTATTGTACGCAGTCTTACCCAGTTTCTTGGTGTATTGTATGTGGCCTTACCCGGTGCACAAAATTTTAAATCATACACACTGATAATCAATTGTCCTCCTAACTCTCAGATCATTAAAATTGTTTGACTTTAATTATACATAGACCATACGATTGATTTTGATTTGCTGATAGTGTTAAACTAAAAACAATTCTTTGGATTTCGTTTGCAGTATGGATTCCTTTTTTTTTTTTGTATTACTTAATACTTAAGTTCTTTGTTTTGTAGTCATATAAGGTCTGTTTGGAAAGTAAATACAGTATAAAGAGTATCATCACCACAGCTGATGGATATCTTACACCTAAAATCACCAGAATTCTTGGTTTGCATTTGTTTGTTCAATATGGGTTTAATTGATATGCACTTTTACCTATCAATCAATCATAACATGGCTCGAAATATACGCCTTTAATCATGTCTACCTTAAAAGTCATACGAAATATATATTTTGTGACTGTTTAACTACCTTAAAAGTTTTACGACGACGGTGCATCAAAATTAATCTCTTTGAATATTCTCAAAATTGTTAAGAGAACACGTAGATAATCATGTAACATGGTTTGTGCTTGTCAATCAGACTCAGATTCTTCAGCTCCCAAGAGTACTCAATATATCAATCATTGGTGTAATAATATGGCTCTATCTTACCATAATATAATGCTTTTTTGTTTGTCAATAAATAATGAATATCCTTTATTTCTTGAGCAGAAGTATTAATCACTCTTCTAATGAAAAAGTCCTTCACCCCATGATTAACTGAGTTTGATTAAGGAACATGATAGTTTTTGGCTTCTTTTAATTGTATTCATACAATTTAATTTGTCCACAAAAAATTGCTAATATTATCTTCTTTTTTTCAATGAAGGGAGTTGTTGGGCATTCTCAGCAATCGCAGCTGTTGAAGGAATAAATAAGATTGTGACAGGAGATCTAATTTCACTATCAGAACAAGAACTGGTGGACTGTGATACATCATACAACGAAGGATGCAATGGTGGACTTATGGATTACGCCTTTGAGTTCATCATCAGTAATGGTGGCATTGACTCTGAAGAGGATTACCCTTACAAGGCTATTGATGGTAGATGTGACCAGAACAGGGTTAGTTACTTGGTCATTGATTTTTTAATGACAATAGTAAATGTTTGTGGAAGATAGTTAATCAGTAGGACTAGTTAGTTAACACTCTAATTCTTTCATTGGTTTTAAACTGTTTGGTTATTCTCTTTGTGCGCAGAAAAATGCTAAAGTTGTTACAATTGATGACTATGAAGATGTTCCTGCTTATGATGAATTGGCCTTGCAAAAGGCTGTTGCAAACCAGCCTATCGCCGTTGCTGTTGAAGGAGGCGGTAGGGAATTCCAGTTATATGAATATGTAAGTTTCTAAATTAATTCTACTATGAAACTAGGAATTTTATACTGTGTTGCATTGGGATAACGTCATAATCAATACTGCAAACGTGTATAAATTGTTATAACTATCCTTCACATATTGATTTTGAATAAAAATCATCTGTCTGGCAAGTGGTTTTGCTATAACTATCCTTCACATTATCTTCAATAGCCTGTTAATTTATTTTTTTTGACTTTGCTTCACAAGATTGGTACATACTGTTATATTTCATGAACTTTCTGTTTGAATGTATTCCATTTGTCTCTTGTCAGGGTGTATTTACCGGTAGATGTGGAACGGCACTAGACCATGGTGTTGCAGCTGTTGGATACGGTACAGAAAATGGGAAAGATTATTGGATTGTGAGAAATTCATGGGGTGGTAGCTGGGGTGAACAAGGTTACATCAGACTCGAACGTAATCTAGCTTCAAGCAGAGCAGGAAAGTGTGGAATTGCGATCGAGCCATCTTATCCAATTAAGAATGGACAGAACCCCCCTAACCCTGGACCATCACCACCTTCACCTATCAAGCCACCATCTGTCTGTGACAGCTACTACAGCTGTGCTGAAGGCTCTACTTGTTGCTGTATTTACGAGTATGGAAGATCTTGCTTTGAGTGGGGATGCTGTCCTCTTGAGTCTGCTACTTGCTGTGATGATCACTACAGTTGCTGCCCTCATGAGTATCCTGTCTGTGACACTCGTGCTGGACTTTGTCTTAAGGTATGTCCAAGTCAACCTTATCTTTTACTTGCTTGGCATTTTTGATTGGATTTTCACTTGGAACCAATGCTTTAAAAGCCAAACCGGACATTGAAACGTAAAGACTTCAGTCATGGTTCAACCGTTTTAAACAGCTCAAATTGGAATGAAACCACGATCAAACAGTTTAAATAACCGAACCGTAGATAGTGTCTGTTCATTGCTCAACTGGTTGAACTGTCTTGTCTGGTTCCATTTTTAAAACATTACTTGGAACTAATTGTTGGCTACTATTTTGGATTTTTTTCCTGTTGATATGTCCCTTTTGCGTTCATTTGGACTTTTTGAATTATATTTTATGTTTTCAGTTAAAATATGATTAGTTTTAGCAGAAAAAACAATTTCTCAATGTTAAGTGTTTGTAAGTGAGTTAAATGAGATAGTTTTCTTAAAAAATTGAGATGCATTATGCATATTATGTTTAGAAGAGCTTATAAAGATAGCTTATAATATATCCATAAGCTGGTTTCAGATTATCGTCATAAGTTTTATGTGATAGCCTATAAATTTTTTATAAAAAGGCTTATGCACTATGCAGAGTTAAACTAATACTCCCTCCGTTTCAAAATGAGTGTCGCTTTAGCCAAAAAAATTTGTTTCAAAATGAATGTCACTTTCAGTTTCCAATGCAATAATAACTTTTTCTTTTCAATTGTACCCTCCAATTAATGCTTTGCACACTACTTCCAAAGTACTACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTGGTAAAATAATCATTTTCTTTCTTTTAATCATTGCATTTCTTAATATGTGTGTAAACACCTAAAACGACACTCATTTTGAAACAGAGGGAGTAAGCTCTAAGTATTATATTATTTGAGTTTATCTGCTGAGACTGTTTGGGAGAACATGGAAAAATAGCCTATAACATGTCTATTATTCGTTTTCTTAATATTTTCATAATTCAGAATAACTTAAGAATACAACTTATAGCTTGAATAAAAACAATTCGACTTTATCCAAACAAAGTCAAATTCTTATGGGAAAAGAACTTATAGAACAAGCTCTTAATTTAGTTGTTTTATCGGGAATTATCCAAATTTGTATGGATTGTTTACACTTTTGCTTATCCAAATTTTTATGTGCTTGATCACAGGGCAAGAATAACCCATTGGGAGTGAAGTCATTCAAACGCACTCCTGCCAAGCCTCACTGGGCCTTTGGTGGTAAGAACAAGATGAGCAATGCTTAA
Protein sequence

>MS.gene00463.t1

MDSAMKLMIVLILATFTVSLALDMSIISYDKTHPDKSTSKRTNEEVLTMYEEWLVKHGKSYNGLGEKDKRFEIFKDNLKFIDEHNGLNSTYRLGLTRFADLTNEEYRSKFLGTKIDPNRRMKKLGGVRSNRYAPRVGDKLPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRCDQNRKNAKVVTIDDYEDVPAYDELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIVRNSWGGSWGEQGYIRLERNLASSRAGKCGIAIEPSYPIKNGQNPPNPGPSPPSPIKPPSVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESATCCDDHYSCCPHEYPVCDTRAGLCLKGKNNPLGVKSFKRTPAKPHWAFGGKNKMSNA