AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004647

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004647.t1 MTR_2g437700 98.681 379 4 1 1 379 12 389 0.0 737
MS.gene004647.t1 MTR_7g075453 32.759 348 214 11 16 361 23 352 6.52e-37 138
MS.gene004647.t1 MTR_2g018340 41.558 154 84 3 182 332 37 187 1.33e-31 119
MS.gene004647.t1 MTR_3g460780 28.840 319 213 10 16 333 31 336 9.25e-26 107
MS.gene004647.t1 MTR_5g098420 27.726 321 212 11 16 333 35 338 6.41e-22 97.1
MS.gene004647.t1 MTR_7g101080 31.677 161 101 4 179 335 35 190 4.45e-17 80.5
MS.gene004647.t1 MTR_5g098060 34.615 182 106 5 182 360 46 217 5.70e-17 80.5
MS.gene004647.t1 MTR_2g018380 36.066 122 72 3 230 345 53 174 1.40e-16 77.8
MS.gene004647.t1 MTR_4g059840 32.240 183 110 6 183 360 72 245 4.41e-15 75.5
MS.gene004647.t1 MTR_2g093980 29.221 154 102 3 184 334 69 218 2.36e-14 73.2
MS.gene004647.t1 MTR_2g017950 27.749 191 125 4 183 364 70 256 3.04e-14 73.2
MS.gene004647.t1 MTR_4g053380 31.667 180 110 6 183 355 76 249 3.92e-14 72.8
MS.gene004647.t1 MTR_4g059790 30.387 181 116 5 183 360 72 245 4.28e-14 72.4
MS.gene004647.t1 MTR_8g087460 30.769 156 97 4 183 332 92 242 4.97e-14 72.8
MS.gene004647.t1 MTR_8g087470 30.769 156 97 4 183 332 92 242 5.56e-14 72.4
MS.gene004647.t1 MTR_2g090765 29.730 185 121 5 153 334 8 186 9.41e-13 67.8
MS.gene004647.t1 MTR_8g087450 31.544 149 95 4 184 329 77 221 1.35e-12 68.2
MS.gene004647.t1 MTR_2g093970 26.943 193 120 5 184 359 69 257 2.99e-12 67.0
MS.gene004647.t1 MTR_4g059720 27.778 162 108 4 174 333 35 189 3.89e-12 66.2
MS.gene004647.t1 MTR_2g017970 29.213 178 117 6 184 358 68 239 5.83e-12 66.2
MS.gene004647.t1 MTR_2g017960 29.213 178 117 6 184 358 68 239 8.22e-12 65.5
MS.gene004647.t1 MTR_2g093990 28.947 152 102 3 184 333 41 188 1.18e-11 64.7
MS.gene004647.t1 MTR_1g009950 28.161 174 114 5 14 184 23 188 2.67e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004647.t1 AT3G46550 55.202 346 137 5 15 346 29 370 1.15e-119 353
MS.gene004647.t1 AT2G45470 33.333 324 202 10 14 336 25 335 1.91e-35 134
MS.gene004647.t1 AT3G60900 32.471 348 213 11 16 354 27 361 6.49e-31 122
MS.gene004647.t1 AT5G55730 29.538 325 213 11 10 333 20 329 9.71e-26 107
MS.gene004647.t1 AT5G55730 29.538 325 213 11 10 333 20 329 9.71e-26 107
MS.gene004647.t1 AT4G12730 28.358 335 223 12 1 333 13 332 6.35e-24 102
MS.gene004647.t1 AT5G44130 29.798 198 132 5 183 378 35 227 4.42e-18 83.2
MS.gene004647.t1 AT5G60490 31.579 152 99 3 184 333 39 187 3.09e-17 80.9
MS.gene004647.t1 AT1G03870 29.282 181 119 4 183 359 38 213 2.43e-15 75.5
MS.gene004647.t1 AT2G20520 31.646 158 97 5 183 334 37 189 3.55e-14 72.0
MS.gene004647.t1 AT5G03170 33.962 159 99 4 179 334 31 186 1.53e-13 70.1
MS.gene004647.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.43e-13 69.7
MS.gene004647.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.43e-13 69.7
MS.gene004647.t1 AT3G12660 31.210 157 103 3 10 166 19 170 4.42e-13 68.9

Find 147 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAATGGTGTTGGTGTTC+GGG 0.251448 2.2:+51514156 None:intergenic
GATTAACACTGGCATTGTTC+AGG 0.317584 2.2:-51513634 MS.gene004647:CDS
TTGTTGCCAAGAGAGATATT+TGG 0.326646 2.2:-51513549 MS.gene004647:CDS
GAGGTGAATGTTGATAATTC+AGG 0.334884 2.2:+51514455 None:intergenic
CTTTCTTCACCGCCAGGGTT+TGG 0.356296 2.2:-51513420 MS.gene004647:CDS
GCTTCAATGCTCTCCGCTTC+CGG 0.361932 2.2:-51513933 MS.gene004647:CDS
GTGAGGGTCGGTGGTGAGAT+AGG 0.366350 2.2:+51514355 None:intergenic
TGTTGCCAAGAGAGATATTT+GGG 0.370591 2.2:-51513548 MS.gene004647:CDS
ATGGTGTTGGGGATAGGATT+AGG 0.379163 2.2:+51514516 None:intergenic
GTGAGCATCGATGAGGGTTT+TGG 0.379755 2.2:+51513971 None:intergenic
GGTCGGACCAGGAGAGGAAC+TGG 0.383020 2.2:+51514276 None:intergenic
ATTGTAATGGTGTTGGTGTT+CGG 0.388000 2.2:+51514155 None:intergenic
TGGTGTTGGTGTTCGGGGAG+TGG 0.394617 2.2:+51514162 None:intergenic
GATGTCAGCGAGAGCGGAGG+GGG 0.395262 2.2:+51514319 None:intergenic
TGGTGGCCCGGCCGGTGGTT+TGG 0.397807 2.2:+51514210 None:intergenic
TCCTCCTCCAGTTCCTCTCC+TGG 0.402978 2.2:-51514283 MS.gene004647:CDS
GGTGTTGGTGTTCGGGGAGT+GGG 0.411168 2.2:+51514163 None:intergenic
TGAAAGAGAAAGATGGTGTT+GGG 0.411336 2.2:+51514504 None:intergenic
GGAGATTTCGCAGACACAAT+TGG 0.413692 2.2:+51513519 None:intergenic
CTTCGTGCTTATTATCCTCT+CGG 0.417892 2.2:-51513762 MS.gene004647:CDS
GATTCTCTCTTAATCCCTTA+CGG 0.421971 2.2:-51514044 MS.gene004647:CDS
GGCCGGGCCACCAACAACTT+TGG 0.425079 2.2:-51514200 MS.gene004647:CDS
GTCGGTGGTGAGATAGGTGT+TGG 0.428158 2.2:+51514361 None:intergenic
AAGAGTAAGGTGCTGGGGAA+TGG 0.428506 2.2:+51514132 None:intergenic
GGGGAGAGGTTATGGAGGTG+AGG 0.434679 2.2:+51514338 None:intergenic
TTCTTCCCAAATATCTCTCT+TGG 0.439416 2.2:+51513543 None:intergenic
TGAAAGAACGGTGGCGTTTG+AGG 0.443446 2.2:+51514109 None:intergenic
GAAGCCGGTGAAGGTGGTTC+CGG 0.446950 2.2:-51513894 MS.gene004647:CDS
GAGGTGTTGGAGGTCGGACC+AGG 0.448627 2.2:+51514265 None:intergenic
AGAAAGATGGTGTTGGGGAT+AGG 0.453694 2.2:+51514510 None:intergenic
GAGAGTGGTGACGAGTTTAC+CGG 0.456170 2.2:+51514235 None:intergenic
AGAGGAACTGGAGGAGGATG+TGG 0.458663 2.2:+51514288 None:intergenic
TCGGCGAAAGCATCGTCTAC+GGG 0.459267 2.2:+51513855 None:intergenic
CGGAGGGAGGGAGGTGTTGG+AGG 0.464496 2.2:+51514255 None:intergenic
CCCTTACGGTTTCGATCTCA+TGG 0.465462 2.2:-51514030 MS.gene004647:CDS
GATGCTGCTTCTTCGCCGGC+GGG 0.465731 2.2:-51513465 MS.gene004647:CDS
ATTAACACTGGCATTGTTCA+GGG 0.469145 2.2:-51513633 MS.gene004647:CDS
GGTGAAGATAGTGAGATTGT+AGG 0.469199 2.2:+51514070 None:intergenic
GACACTTGCGACGGAAGCTA+TGG 0.469462 2.2:-51513706 MS.gene004647:CDS
TCACATCTTTCTTCACCGCC+AGG 0.471478 2.2:-51513426 MS.gene004647:CDS
CTCTCTTCCAAACCACCGGC+CGG 0.475597 2.2:-51514217 MS.gene004647:CDS
TTCAGGGAAGGTTGAAAGGA+GGG 0.476637 2.2:+51514472 None:intergenic
AGGTGAATGTTGATAATTCA+GGG 0.477819 2.2:+51514456 None:intergenic
AGCCAAAGTTGTTGGTGGCC+CGG 0.478504 2.2:+51514198 None:intergenic
GGGAGAGATCAGCGGTGAGA+GGG 0.486859 2.2:+51514414 None:intergenic
GAAACACCGAAAATCGCAAT+AGG 0.488328 2.2:+51513579 None:intergenic
TAGTGCTCCTCCTCCTTATG+AGG 0.491845 2.2:-51513487 MS.gene004647:CDS
TCGGTGGTGAGATAGGTGTT+GGG 0.492560 2.2:+51514362 None:intergenic
TTTGAGGAAGAGTAAGGTGC+TGG 0.494680 2.2:+51514125 None:intergenic
AAAGTTGTTGGTGGCCCGGC+CGG 0.495578 2.2:+51514202 None:intergenic
TACCGGAGGGAGGGAGGTGT+TGG 0.497410 2.2:+51514252 None:intergenic
CGGGGAGTGGGTGATGTTGA+CGG 0.497837 2.2:+51514175 None:intergenic
TTCATTCACGACGCCGGAAG+CGG 0.499715 2.2:+51513920 None:intergenic
GAAGAAGCAGCATCCTCATA+AGG 0.500145 2.2:+51513474 None:intergenic
GTTGTTGGTGGCCCGGCCGG+TGG 0.501570 2.2:+51514205 None:intergenic
AGGATGTCAGCGAGAGCGGA+GGG 0.508490 2.2:+51514317 None:intergenic
GTCGCAAGTGTCGGTTGCAA+CGG 0.515529 2.2:+51513717 None:intergenic
AGGTTGAAAGGAGGGAGGTG+AGG 0.516160 2.2:+51514480 None:intergenic
ATAATTCAGGGAAGGTTGAA+AGG 0.519692 2.2:+51514468 None:intergenic
ATCGGCGAAAGCATCGTCTA+CGG 0.521274 2.2:+51513854 None:intergenic
GGGGATAAACAAGGTGATAC+CGG 0.523824 2.2:+51513875 None:intergenic
CTCCAACACCTCCCTCCCTC+CGG 0.523989 2.2:-51514254 MS.gene004647:CDS
TTGAAAGAGAAAGATGGTGT+TGG 0.524791 2.2:+51514503 None:intergenic
CACATCTTTCTTCACCGCCA+GGG 0.525421 2.2:-51513425 MS.gene004647:CDS
GCGACGGAAGCTATGGGTGC+TGG 0.525814 2.2:-51513699 MS.gene004647:CDS
GTTATGGAGGTGAGGGTCGG+TGG 0.527291 2.2:+51514346 None:intergenic
GATACCGGAACCACCTTCAC+CGG 0.527970 2.2:+51513890 None:intergenic
GTGAAGATAGTGAGATTGTA+GGG 0.528446 2.2:+51514071 None:intergenic
GGGAGGGAGGTGTTGGAGGT+CGG 0.529487 2.2:+51514259 None:intergenic
GGAGGAGGAGCACTACTCTC+CGG 0.529688 2.2:+51513495 None:intergenic
ATTCAGGGAAGGTTGAAAGG+AGG 0.530201 2.2:+51514471 None:intergenic
TGGGAGAGATCAGCGGTGAG+AGG 0.533298 2.2:+51514413 None:intergenic
TCTCTTCCAAACCACCGGCC+GGG 0.536880 2.2:-51514216 MS.gene004647:CDS
GCGGAGGATGTCAGCGAGAG+CGG 0.537341 2.2:+51514313 None:intergenic
AATGAATTCGAAGCCGGTGA+AGG 0.538116 2.2:-51513903 MS.gene004647:CDS
ACTGGAGGAGGATGTGGTAG+CGG 0.540375 2.2:+51514294 None:intergenic
GGAGAGAGAGTGAATTGCGG+TGG 0.541485 2.2:+51514393 None:intergenic
GGAATGGATTGTAATGGTGT+TGG 0.543280 2.2:+51514148 None:intergenic
GTTGACGGAGCCAAAGTTGT+TGG 0.548182 2.2:+51514190 None:intergenic
GAGGATGTCAGCGAGAGCGG+AGG 0.550692 2.2:+51514316 None:intergenic
GGGAGAGGTTATGGAGGTGA+GGG 0.552715 2.2:+51514339 None:intergenic
TGCTGGGGAATGGATTGTAA+TGG 0.553637 2.2:+51514142 None:intergenic
GGATGCTGCTTCTTCGCCGG+CGG 0.553952 2.2:-51513466 MS.gene004647:CDS
TGTAATGGTGTTGGTGTTCG+GGG 0.554234 2.2:+51514157 None:intergenic
ACCAGGAGAGGAACTGGAGG+AGG 0.555679 2.2:+51514282 None:intergenic
CGGTTTCGATCTCATGGCAT+CGG 0.555806 2.2:-51514024 MS.gene004647:CDS
TTCGAATTCATTCACGACGC+CGG 0.555834 2.2:+51513914 None:intergenic
GTGATGTTGAGAAGAATGCT+TGG 0.555902 2.2:+51513993 None:intergenic
GCCATGAGATCGAAACCGTA+AGG 0.558245 2.2:+51514029 None:intergenic
GTGGCGTTTGAGGAAGAGTA+AGG 0.559294 2.2:+51514119 None:intergenic
GAGGTTATGGAGGTGAGGGT+CGG 0.559779 2.2:+51514343 None:intergenic
GTGAGGTTGAAAGAGAAAGA+TGG 0.560128 2.2:+51514497 None:intergenic
TGAGGATGCTGCTTCTTCGC+CGG 0.566290 2.2:-51513469 MS.gene004647:CDS
CGAAACGGAAGGAGGTAGAT+CGG 0.567781 2.2:+51513836 None:intergenic
TTGAGGAAGAGTAAGGTGCT+GGG 0.571039 2.2:+51514126 None:intergenic
GAGAGATCAGCGGTGAGAGG+GGG 0.573409 2.2:+51514416 None:intergenic
GGAGAGATCAGCGGTGAGAG+GGG 0.577869 2.2:+51514415 None:intergenic
GAATTCGAAGCCGGTGAAGG+TGG 0.580678 2.2:-51513900 MS.gene004647:CDS
GACGGAGCCAAAGTTGTTGG+TGG 0.580994 2.2:+51514193 None:intergenic
GGCTCTGTTGCGATTAACAC+TGG 0.583502 2.2:-51513645 MS.gene004647:CDS
CGGACCAGGAGAGGAACTGG+AGG 0.586171 2.2:+51514279 None:intergenic
GAGAGAGAGTGAATTGCGGT+GGG 0.591977 2.2:+51514394 None:intergenic
ACACTTGCGACGGAAGCTAT+GGG 0.597246 2.2:-51513705 MS.gene004647:CDS
GGGAAGTGATTGAAGCGAAA+CGG 0.597351 2.2:+51513821 None:intergenic
ACCACTCTCTTCCAAACCAC+CGG 0.598222 2.2:-51514221 MS.gene004647:CDS
GAAATTGTGAGCATCGATGA+GGG 0.603493 2.2:+51513965 None:intergenic
GAATGTTGATAATTCAGGGA+AGG 0.603611 2.2:+51514460 None:intergenic
ATAGCTTCCGTCGCAAGTGT+CGG 0.604769 2.2:+51513708 None:intergenic
GGTGAGATAGGTGTTGGGGA+CGG 0.604958 2.2:+51514367 None:intergenic
TGACGAGTTTACCGGAGGGA+GGG 0.605757 2.2:+51514243 None:intergenic
GCAGCATCCTCATAAGGAGG+AGG 0.606713 2.2:+51513480 None:intergenic
TGAAATTGTGAGCATCGATG+AGG 0.610224 2.2:+51513964 None:intergenic
GGATGTCAGCGAGAGCGGAG+GGG 0.610527 2.2:+51514318 None:intergenic
CGAGTTTACCGGAGGGAGGG+AGG 0.611461 2.2:+51514246 None:intergenic
TGTGTCTGCGAAATCTCCAC+CGG 0.612137 2.2:-51513514 MS.gene004647:CDS
AGTGATTGAAGCGAAACGGA+AGG 0.612745 2.2:+51513825 None:intergenic
CGGTTGCAACGGATTCGCAG+TGG 0.617668 2.2:+51513728 None:intergenic
AGGGAAGGTTGAAAGGAGGG+AGG 0.618290 2.2:+51514475 None:intergenic
CGGTGGTGAGATAGGTGTTG+GGG 0.618735 2.2:+51514363 None:intergenic
GTCGTGAATGAATTCGAAGC+CGG 0.623460 2.2:-51513909 MS.gene004647:CDS
GGCTGTACATCAAAACCCGC+CGG 0.624496 2.2:+51513450 None:intergenic
GCCGGTGGTTTGGAAGAGAG+TGG 0.626248 2.2:+51514220 None:intergenic
CGGCGAAAGCATCGTCTACG+GGG 0.626762 2.2:+51513856 None:intergenic
AGGGATTAAGAGAGAATCAA+CGG 0.629656 2.2:+51514049 None:intergenic
TTAACACTGGCATTGTTCAG+GGG 0.631498 2.2:-51513632 MS.gene004647:CDS
GGAGGAGGATGTGGTAGCGG+AGG 0.631708 2.2:+51514297 None:intergenic
GTGACGAGTTTACCGGAGGG+AGG 0.632618 2.2:+51514242 None:intergenic
CCATGAGATCGAAACCGTAA+GGG 0.633243 2.2:+51514030 None:intergenic
GAAAGAGAAAGATGGTGTTG+GGG 0.638474 2.2:+51514505 None:intergenic
GGAGGAGCACTACTCTCCGG+TGG 0.642609 2.2:+51513498 None:intergenic
AGTGGTGACGAGTTTACCGG+AGG 0.642661 2.2:+51514238 None:intergenic
GGGTGTGGAAGAAAGTAGGG+AGG 0.644752 2.2:+51514436 None:intergenic
GTTGGAGGTCGGACCAGGAG+AGG 0.645093 2.2:+51514270 None:intergenic
ATCGTCTACGGGGATAAACA+AGG 0.645330 2.2:+51513866 None:intergenic
GTGGATTGAAGCGAACCGAG+AGG 0.652533 2.2:+51513747 None:intergenic
CTAACATCTTCACCAAACCC+TGG 0.655235 2.2:+51513408 None:intergenic
TGAGGAAGAGTAAGGTGCTG+GGG 0.655459 2.2:+51514127 None:intergenic
GGCGGTGAAGAAAGATGTGA+CGG 0.657561 2.2:+51513429 None:intergenic
GAAGCAGCATCCTCATAAGG+AGG 0.663333 2.2:+51513477 None:intergenic
GTGGTGACGAGTTTACCGGA+GGG 0.663417 2.2:+51514239 None:intergenic
GATAGGTGTTGGGGACGGCG+AGG 0.663521 2.2:+51514372 None:intergenic
GATTGAAGCGAAACGGAAGG+AGG 0.667934 2.2:+51513828 None:intergenic
GTTGCAACCGACACTTGCGA+CGG 0.682389 2.2:-51513715 MS.gene004647:CDS
CGAGGAGAGAGAGTGAATTG+CGG 0.703819 2.2:+51514390 None:intergenic
ATTGCGGTGGGAGAGATCAG+CGG 0.710115 2.2:+51514406 None:intergenic
TTTGAGCTGTGAAAGAACGG+TGG 0.719224 2.2:+51514100 None:intergenic
ACATCTTCACCAAACCCTGG+CGG 0.727699 2.2:+51513411 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATATTTCAACTTTTAA+TGG - chr2.2:51514183-51514202 MS.gene004647:CDS 15.0%
!! AGGTGAATGTTGATAATTCA+GGG + chr2.2:51513396-51513415 None:intergenic 30.0%
!!! AAGAACAACTGCTTTTTGAT+CGG + chr2.2:51514055-51514074 None:intergenic 30.0%
AGGGATTAAGAGAGAATCAA+CGG + chr2.2:51513803-51513822 None:intergenic 35.0%
ATAATTCAGGGAAGGTTGAA+AGG + chr2.2:51513384-51513403 None:intergenic 35.0%
ATTAACACTGGCATTGTTCA+GGG - chr2.2:51514216-51514235 MS.gene004647:CDS 35.0%
GATTCTCTCTTAATCCCTTA+CGG - chr2.2:51513805-51513824 MS.gene004647:CDS 35.0%
GTGAAGATAGTGAGATTGTA+GGG + chr2.2:51513781-51513800 None:intergenic 35.0%
TCAGATGTTGCAAGTGTTTA+TGG - chr2.2:51514465-51514484 MS.gene004647:CDS 35.0%
TGAAAGAGAAAGATGGTGTT+GGG + chr2.2:51513348-51513367 None:intergenic 35.0%
TGTTGCCAAGAGAGATATTT+GGG - chr2.2:51514301-51514320 MS.gene004647:CDS 35.0%
TTCTTCCCAAATATCTCTCT+TGG + chr2.2:51514309-51514328 None:intergenic 35.0%
TTGAAAGAGAAAGATGGTGT+TGG + chr2.2:51513349-51513368 None:intergenic 35.0%
TTGTTGCCAAGAGAGATATT+TGG - chr2.2:51514300-51514319 MS.gene004647:CDS 35.0%
! CAGAATCCTATTGCGATTTT+CGG - chr2.2:51514264-51514283 MS.gene004647:CDS 35.0%
! CATTTTGAGCTGTGAAAGAA+CGG + chr2.2:51513755-51513774 None:intergenic 35.0%
!! ATTGTAATGGTGTTGGTGTT+CGG + chr2.2:51513697-51513716 None:intergenic 35.0%
!! GAATGTTGATAATTCAGGGA+AGG + chr2.2:51513392-51513411 None:intergenic 35.0%
!! GAGGTGAATGTTGATAATTC+AGG + chr2.2:51513397-51513416 None:intergenic 35.0%
CTTCGTGCTTATTATCCTCT+CGG - chr2.2:51514087-51514106 MS.gene004647:CDS 40.0%
GAAACACCGAAAATCGCAAT+AGG + chr2.2:51514273-51514292 None:intergenic 40.0%
GATTAACACTGGCATTGTTC+AGG - chr2.2:51514215-51514234 MS.gene004647:CDS 40.0%
GGTGAAGATAGTGAGATTGT+AGG + chr2.2:51513782-51513801 None:intergenic 40.0%
GTGAGGTTGAAAGAGAAAGA+TGG + chr2.2:51513355-51513374 None:intergenic 40.0%
GTGATGTTGAGAAGAATGCT+TGG + chr2.2:51513859-51513878 None:intergenic 40.0%
TTAACACTGGCATTGTTCAG+GGG - chr2.2:51514217-51514236 MS.gene004647:CDS 40.0%
! GAAAGAGAAAGATGGTGTTG+GGG + chr2.2:51513347-51513366 None:intergenic 40.0%
!! GAAATTGTGAGCATCGATGA+GGG + chr2.2:51513887-51513906 None:intergenic 40.0%
!! GGAATGGATTGTAATGGTGT+TGG + chr2.2:51513704-51513723 None:intergenic 40.0%
!! TGAAATTGTGAGCATCGATG+AGG + chr2.2:51513888-51513907 None:intergenic 40.0%
!! TTGTAATGGTGTTGGTGTTC+GGG + chr2.2:51513696-51513715 None:intergenic 40.0%
!!! AACAACTGCTTTTTGATCGG+CGG + chr2.2:51514052-51514071 None:intergenic 40.0%
AATGAATTCGAAGCCGGTGA+AGG - chr2.2:51513946-51513965 MS.gene004647:CDS 45.0%
AGTGATTGAAGCGAAACGGA+AGG + chr2.2:51514027-51514046 None:intergenic 45.0%
ATCGTCTACGGGGATAAACA+AGG + chr2.2:51513986-51514005 None:intergenic 45.0%
ATTCAGGGAAGGTTGAAAGG+AGG + chr2.2:51513381-51513400 None:intergenic 45.0%
CCATGAGATCGAAACCGTAA+GGG + chr2.2:51513822-51513841 None:intergenic 45.0%
CTAACATCTTCACCAAACCC+TGG + chr2.2:51514444-51514463 None:intergenic 45.0%
GAAGAAGCAGCATCCTCATA+AGG + chr2.2:51514378-51514397 None:intergenic 45.0%
GGAGATTTCGCAGACACAAT+TGG + chr2.2:51514333-51514352 None:intergenic 45.0%
GGGAAGTGATTGAAGCGAAA+CGG + chr2.2:51514031-51514050 None:intergenic 45.0%
GGGGATAAACAAGGTGATAC+CGG + chr2.2:51513977-51513996 None:intergenic 45.0%
GTCGTGAATGAATTCGAAGC+CGG - chr2.2:51513940-51513959 MS.gene004647:CDS 45.0%
TTCAGGGAAGGTTGAAAGGA+GGG + chr2.2:51513380-51513399 None:intergenic 45.0%
TTCGAATTCATTCACGACGC+CGG + chr2.2:51513938-51513957 None:intergenic 45.0%
TTTGAGCTGTGAAAGAACGG+TGG + chr2.2:51513752-51513771 None:intergenic 45.0%
TTTGAGGAAGAGTAAGGTGC+TGG + chr2.2:51513727-51513746 None:intergenic 45.0%
! AGAAAGATGGTGTTGGGGAT+AGG + chr2.2:51513342-51513361 None:intergenic 45.0%
! ATGGTGTTGGGGATAGGATT+AGG + chr2.2:51513336-51513355 None:intergenic 45.0%
! TTGAGGAAGAGTAAGGTGCT+GGG + chr2.2:51513726-51513745 None:intergenic 45.0%
!! TGCTGGGGAATGGATTGTAA+TGG + chr2.2:51513710-51513729 None:intergenic 45.0%
!! TGTAATGGTGTTGGTGTTCG+GGG + chr2.2:51513695-51513714 None:intergenic 45.0%
!!! ACAACTGCTTTTTGATCGGC+GGG + chr2.2:51514051-51514070 None:intergenic 45.0%
!!! TGCGATTTTCGGTGTTTCGA+AGG - chr2.2:51514275-51514294 MS.gene004647:CDS 45.0%
ACACTTGCGACGGAAGCTAT+GGG - chr2.2:51514144-51514163 MS.gene004647:CDS 50.0%
ACATCTTCACCAAACCCTGG+CGG + chr2.2:51514441-51514460 None:intergenic 50.0%
ACCACTCTCTTCCAAACCAC+CGG - chr2.2:51513628-51513647 MS.gene004647:CDS 50.0%
ATAGCTTCCGTCGCAAGTGT+CGG + chr2.2:51514144-51514163 None:intergenic 50.0%
CACATCTTTCTTCACCGCCA+GGG - chr2.2:51514424-51514443 MS.gene004647:CDS 50.0%
CCCTTACGGTTTCGATCTCA+TGG - chr2.2:51513819-51513838 MS.gene004647:CDS 50.0%
CGAAACGGAAGGAGGTAGAT+CGG + chr2.2:51514016-51514035 None:intergenic 50.0%
CGAGGAGAGAGAGTGAATTG+CGG + chr2.2:51513462-51513481 None:intergenic 50.0%
CGGTTTCGATCTCATGGCAT+CGG - chr2.2:51513825-51513844 MS.gene004647:CDS 50.0%
GAAGCAGCATCCTCATAAGG+AGG + chr2.2:51514375-51514394 None:intergenic 50.0%
GAGAGAGAGTGAATTGCGGT+GGG + chr2.2:51513458-51513477 None:intergenic 50.0%
GATTGAAGCGAAACGGAAGG+AGG + chr2.2:51514024-51514043 None:intergenic 50.0%
GCCATGAGATCGAAACCGTA+AGG + chr2.2:51513823-51513842 None:intergenic 50.0%
GGCGGTGAAGAAAGATGTGA+CGG + chr2.2:51514423-51514442 None:intergenic 50.0%
GGCTCTGTTGCGATTAACAC+TGG - chr2.2:51514204-51514223 MS.gene004647:CDS 50.0%
GTGGCGTTTGAGGAAGAGTA+AGG + chr2.2:51513733-51513752 None:intergenic 50.0%
GTTGACGGAGCCAAAGTTGT+TGG + chr2.2:51513662-51513681 None:intergenic 50.0%
TAGTGCTCCTCCTCCTTATG+AGG - chr2.2:51514362-51514381 MS.gene004647:CDS 50.0%
TCACATCTTTCTTCACCGCC+AGG - chr2.2:51514423-51514442 MS.gene004647:CDS 50.0%
TCGGTGGTGAGATAGGTGTT+GGG + chr2.2:51513490-51513509 None:intergenic 50.0%
TGAAAGAACGGTGGCGTTTG+AGG + chr2.2:51513743-51513762 None:intergenic 50.0%
TGTGTCTGCGAAATCTCCAC+CGG - chr2.2:51514335-51514354 MS.gene004647:CDS 50.0%
! AGGGGGTGTGGAAGAAAGTA+GGG + chr2.2:51513419-51513438 None:intergenic 50.0%
! GAGAGTGGTGACGAGTTTAC+CGG + chr2.2:51513617-51513636 None:intergenic 50.0%
!! AAGAGTAAGGTGCTGGGGAA+TGG + chr2.2:51513720-51513739 None:intergenic 50.0%
!! ATCGGCGAAAGCATCGTCTA+CGG + chr2.2:51513998-51514017 None:intergenic 50.0%
!! TGAGGAAGAGTAAGGTGCTG+GGG + chr2.2:51513725-51513744 None:intergenic 50.0%
!!! GTGAGCATCGATGAGGGTTT+TGG + chr2.2:51513881-51513900 None:intergenic 50.0%
ACTGGAGGAGGATGTGGTAG+CGG + chr2.2:51513558-51513577 None:intergenic 55.0%
AGAGGAACTGGAGGAGGATG+TGG + chr2.2:51513564-51513583 None:intergenic 55.0%
AGGGAAGGTTGAAAGGAGGG+AGG + chr2.2:51513377-51513396 None:intergenic 55.0%
AGGTTGAAAGGAGGGAGGTG+AGG + chr2.2:51513372-51513391 None:intergenic 55.0%
ATTGCGGTGGGAGAGATCAG+CGG + chr2.2:51513446-51513465 None:intergenic 55.0%
CTTTCTTCACCGCCAGGGTT+TGG - chr2.2:51514429-51514448 MS.gene004647:CDS 55.0%
GAATTCGAAGCCGGTGAAGG+TGG - chr2.2:51513949-51513968 MS.gene004647:CDS 55.0%
GACACTTGCGACGGAAGCTA+TGG - chr2.2:51514143-51514162 MS.gene004647:CDS 55.0%
GACGGAGCCAAAGTTGTTGG+TGG + chr2.2:51513659-51513678 None:intergenic 55.0%
GAGGTTATGGAGGTGAGGGT+CGG + chr2.2:51513509-51513528 None:intergenic 55.0%
GATACCGGAACCACCTTCAC+CGG + chr2.2:51513962-51513981 None:intergenic 55.0%
GCAGCATCCTCATAAGGAGG+AGG + chr2.2:51514372-51514391 None:intergenic 55.0%
GCTTCAATGCTCTCCGCTTC+CGG - chr2.2:51513916-51513935 MS.gene004647:CDS 55.0%
GGAGAGAGAGTGAATTGCGG+TGG + chr2.2:51513459-51513478 None:intergenic 55.0%
GGCTGTACATCAAAACCCGC+CGG + chr2.2:51514402-51514421 None:intergenic 55.0%
GGGAGAGGTTATGGAGGTGA+GGG + chr2.2:51513513-51513532 None:intergenic 55.0%
GGGTGTGGAAGAAAGTAGGG+AGG + chr2.2:51513416-51513435 None:intergenic 55.0%
GTCGGTGGTGAGATAGGTGT+TGG + chr2.2:51513491-51513510 None:intergenic 55.0%
GTTGCAACCGACACTTGCGA+CGG - chr2.2:51514134-51514153 MS.gene004647:CDS 55.0%
TGACGAGTTTACCGGAGGGA+GGG + chr2.2:51513609-51513628 None:intergenic 55.0%
TTCATTCACGACGCCGGAAG+CGG + chr2.2:51513932-51513951 None:intergenic 55.0%
! AGTGGTGACGAGTTTACCGG+AGG + chr2.2:51513614-51513633 None:intergenic 55.0%
! CGGTGGTGAGATAGGTGTTG+GGG + chr2.2:51513489-51513508 None:intergenic 55.0%
! GAGGGGGTGTGGAAGAAAGT+AGG + chr2.2:51513420-51513439 None:intergenic 55.0%
! GTGGATTGAAGCGAACCGAG+AGG + chr2.2:51514105-51514124 None:intergenic 55.0%
! GTGGTGACGAGTTTACCGGA+GGG + chr2.2:51513613-51513632 None:intergenic 55.0%
! TGAGGATGCTGCTTCTTCGC+CGG - chr2.2:51514380-51514399 MS.gene004647:CDS 55.0%
!! AGCCAAAGTTGTTGGTGGCC+CGG + chr2.2:51513654-51513673 None:intergenic 55.0%
!! GGTGAGATAGGTGTTGGGGA+CGG + chr2.2:51513485-51513504 None:intergenic 55.0%
!! GTCGCAAGTGTCGGTTGCAA+CGG + chr2.2:51514135-51514154 None:intergenic 55.0%
!! TCGGCGAAAGCATCGTCTAC+GGG + chr2.2:51513997-51514016 None:intergenic 55.0%
ACCAGGAGAGGAACTGGAGG+AGG + chr2.2:51513570-51513589 None:intergenic 60.0%
AGGATGTCAGCGAGAGCGGA+GGG + chr2.2:51513535-51513554 None:intergenic 60.0%
CGGGGAGTGGGTGATGTTGA+CGG + chr2.2:51513677-51513696 None:intergenic 60.0%
CTCTCTTCCAAACCACCGGC+CGG - chr2.2:51513632-51513651 MS.gene004647:CDS 60.0%
GAAGCCGGTGAAGGTGGTTC+CGG - chr2.2:51513955-51513974 MS.gene004647:CDS 60.0%
GAGAGATCAGCGGTGAGAGG+GGG + chr2.2:51513436-51513455 None:intergenic 60.0%
GGAGAGATCAGCGGTGAGAG+GGG + chr2.2:51513437-51513456 None:intergenic 60.0%
GGGAGAGATCAGCGGTGAGA+GGG + chr2.2:51513438-51513457 None:intergenic 60.0%
GGGGAGAGGTTATGGAGGTG+AGG + chr2.2:51513514-51513533 None:intergenic 60.0%
GTGACGAGTTTACCGGAGGG+AGG + chr2.2:51513610-51513629 None:intergenic 60.0%
GTGAGGGTCGGTGGTGAGAT+AGG + chr2.2:51513497-51513516 None:intergenic 60.0%
GTTATGGAGGTGAGGGTCGG+TGG + chr2.2:51513506-51513525 None:intergenic 60.0%
TCCTCCTCCAGTTCCTCTCC+TGG - chr2.2:51513566-51513585 MS.gene004647:CDS 60.0%
TCTCTTCCAAACCACCGGCC+GGG - chr2.2:51513633-51513652 MS.gene004647:CDS 60.0%
TGGGAGAGATCAGCGGTGAG+AGG + chr2.2:51513439-51513458 None:intergenic 60.0%
! CGGTTGCAACGGATTCGCAG+TGG + chr2.2:51514124-51514143 None:intergenic 60.0%
! GCCGGTGGTTTGGAAGAGAG+TGG + chr2.2:51513632-51513651 None:intergenic 60.0%
!! AAAGTTGTTGGTGGCCCGGC+CGG + chr2.2:51513650-51513669 None:intergenic 60.0%
!! CGGCGAAAGCATCGTCTACG+GGG + chr2.2:51513996-51514015 None:intergenic 60.0%
!! GGAGGAGGAGCACTACTCTC+CGG + chr2.2:51514357-51514376 None:intergenic 60.0%
!! GGTGTTGGTGTTCGGGGAGT+GGG + chr2.2:51513689-51513708 None:intergenic 60.0%
!! TGGTGTTGGTGTTCGGGGAG+TGG + chr2.2:51513690-51513709 None:intergenic 60.0%
ATCAGCGGTGAGAGGGGGTG+TGG + chr2.2:51513431-51513450 None:intergenic 65.0%
CGAGTTTACCGGAGGGAGGG+AGG + chr2.2:51513606-51513625 None:intergenic 65.0%
CGGACCAGGAGAGGAACTGG+AGG + chr2.2:51513573-51513592 None:intergenic 65.0%
CGGAGGGGGAGAGGTTATGG+AGG + chr2.2:51513519-51513538 None:intergenic 65.0%
CTCCAACACCTCCCTCCCTC+CGG - chr2.2:51513595-51513614 MS.gene004647:CDS 65.0%
GAGCGGAGGGGGAGAGGTTA+TGG + chr2.2:51513522-51513541 None:intergenic 65.0%
GAGGATGTCAGCGAGAGCGG+AGG + chr2.2:51513536-51513555 None:intergenic 65.0%
GATGTCAGCGAGAGCGGAGG+GGG + chr2.2:51513533-51513552 None:intergenic 65.0%
GCGACGGAAGCTATGGGTGC+TGG - chr2.2:51514150-51514169 MS.gene004647:CDS 65.0%
GCGGAGGATGTCAGCGAGAG+CGG + chr2.2:51513539-51513558 None:intergenic 65.0%
GGATGTCAGCGAGAGCGGAG+GGG + chr2.2:51513534-51513553 None:intergenic 65.0%
GGCCGGGCCACCAACAACTT+TGG - chr2.2:51513649-51513668 MS.gene004647:CDS 65.0%
GGTCGGACCAGGAGAGGAAC+TGG + chr2.2:51513576-51513595 None:intergenic 65.0%
GTTGGAGGTCGGACCAGGAG+AGG + chr2.2:51513582-51513601 None:intergenic 65.0%
! GATGCTGCTTCTTCGCCGGC+GGG - chr2.2:51514384-51514403 MS.gene004647:CDS 65.0%
! GGATGCTGCTTCTTCGCCGG+CGG - chr2.2:51514383-51514402 MS.gene004647:CDS 65.0%
! TACCGGAGGGAGGGAGGTGT+TGG + chr2.2:51513600-51513619 None:intergenic 65.0%
!! GAGGTGTTGGAGGTCGGACC+AGG + chr2.2:51513587-51513606 None:intergenic 65.0%
!! GATAGGTGTTGGGGACGGCG+AGG + chr2.2:51513480-51513499 None:intergenic 65.0%
!! GGAGGAGCACTACTCTCCGG+TGG + chr2.2:51514354-51514373 None:intergenic 65.0%
!! GGAGGAGGATGTGGTAGCGG+AGG + chr2.2:51513555-51513574 None:intergenic 65.0%
!! GGGAGGGAGGTGTTGGAGGT+CGG + chr2.2:51513593-51513612 None:intergenic 65.0%
!! CGGAGGGAGGGAGGTGTTGG+AGG + chr2.2:51513597-51513616 None:intergenic 70.0%
CAGCGAGAGCGGAGGGGGAG+AGG + chr2.2:51513528-51513547 None:intergenic 75.0%
TGGTGGCCCGGCCGGTGGTT+TGG + chr2.2:51513642-51513661 None:intergenic 75.0%
!! GTTGTTGGTGGCCCGGCCGG+TGG + chr2.2:51513647-51513666 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 51513322 51514549 51513322 ID=MS.gene004647
chr2.2 mRNA 51513322 51514549 51513322 ID=MS.gene004647.t1;Parent=MS.gene004647
chr2.2 exon 51513413 51514549 51513413 ID=MS.gene004647.t1.exon1;Parent=MS.gene004647.t1
chr2.2 CDS 51513413 51514549 51513413 ID=cds.MS.gene004647.t1;Parent=MS.gene004647.t1
chr2.2 exon 51513322 51513333 51513322 ID=MS.gene004647.t1.exon2;Parent=MS.gene004647.t1
chr2.2 CDS 51513322 51513333 51513322 ID=cds.MS.gene004647.t1;Parent=MS.gene004647.t1
Gene Sequence

>MS.gene004647

ATGCTATATTTCCTAATCCTATCCCCAACACCATCTTTCTCTTTCAACCTCACCTCCCTCCTTTCAACCTTCCCTGAATTATCAACATTCACCTCCCTACTTTCTTCCACACCCCCTCTCACCGCTGATCTCTCCCACCGCAATTCACTCTCTCTCCTCGCCGTCCCCAACACCTATCTCACCACCGACCCTCACCTCCATAACCTCTCCCCCTCCGCTCTCGCTGACATCCTCCGCTACCACATCCTCCTCCAGTTCCTCTCCTGGTCCGACCTCCAACACCTCCCTCCCTCCGGTAAACTCGTCACCACTCTCTTCCAAACCACCGGCCGGGCCACCAACAACTTTGGCTCCGTCAACATCACCCACTCCCCGAACACCAACACCATTACAATCCATTCCCCAGCACCTTACTCTTCCTCAAACGCCACCGTTCTTTCACAGCTCAAAATGTTACCCTACAATCTCACTATCTTCACCGTTGATTCTCTCTTAATCCCTTACGGTTTCGATCTCATGGCATCGGAAACTCGTCCAAGCATTCTTCTCAACATCACCAAAACCCTCATCGATGCTCACAATTTCAACGTCGCTGCTTCAATGCTCTCCGCTTCCGGCGTCGTGAATGAATTCGAAGCCGGTGAAGGTGGTTCCGGTATCACCTTGTTTATCCCCGTAGACGATGCTTTCGCCGATCTACCTCCTTCCGTTTCGCTTCAATCACTTCCCGCCGATCAAAAAGCAGTTGTTCTTAAAGCGCATGTTCTTCGTGCTTATTATCCTCTCGGTTCGCTTCAATCCACTGCGAATCCGTTGCAACCGACACTTGCGACGGAAGCTATGGGTGCTGGAAGTTTCACGTTGAATATTTCAACTTTTAATGGCTCTGTTGCGATTAACACTGGCATTGTTCAGGGGATAATTACTCAGACAGTGTTTGATCAGAATCCTATTGCGATTTTCGGTGTTTCGAAGGTTTTGTTGCCAAGAGAGATATTTGGGAAGAATCCAATTGTGTCTGCGAAATCTCCACCGGAGAGTAGTGCTCCTCCTCCTTATGAGGATGCTGCTTCTTCGCCGGCGGGTTTTGATGTACAGCCGTCACATCTTTCTTCACCGCCAGGGTTTGGTGAAGATGTTAGCTCAGATGTTGCAAGTGTTTATGGTTCTGAGTTATTTGTTTTTCTACTTTGTTGTATAAATTTGTATTTAATAGTATAAAGAGTAA
Protein sequence

>MS.gene004647.t1

MLYFLILSPTPSFSFNLTSLLSTFPELSTFTSLLSSTPPLTADLSHRNSLSLLAVPNTYLTTDPHLHNLSPSALADILRYHILLQFLSWSDLQHLPPSGKLVTTLFQTTGRATNNFGSVNITHSPNTNTITIHSPAPYSSSNATVLSQLKMLPYNLTIFTVDSLLIPYGFDLMASETRPSILLNITKTLIDAHNFNVAASMLSASGVVNEFEAGEGGSGITLFIPVDDAFADLPPSVSLQSLPADQKAVVLKAHVLRAYYPLGSLQSTANPLQPTLATEAMGAGSFTLNISTFNGSVAINTGIVQGIITQTVFDQNPIAIFGVSKVLLPREIFGKNPIVSAKSPPESSAPPPYEDAASSPAGFDVQPSHLSSPPGFGEDYKE