Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005977.t1 | XP_003591924.2 | 97.9 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 9.30E-241 | 842.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005977.t1 | Q42884 | 77.9 | 438 | 93 | 2 | 1 | 434 | 1 | 438 | 4.0e-192 | 672.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005977.t1 | G7I3B2 | 97.9 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 6.7e-241 | 842.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22482 | MS.gene005977 | PPI |
| MS.gene005977 | MS.gene09785 | PPI |
| MS.gene061932 | MS.gene005977 | PPI |
| MS.gene005977 | MS.gene007485 | PPI |
| MS.gene007486 | MS.gene005977 | PPI |
| MS.gene007487 | MS.gene005977 | PPI |
| MS.gene018518 | MS.gene005977 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005977.t1 | MTR_1g095250 | 97.936 | 436 | 9 | 0 | 1 | 436 | 1 | 436 | 0.0 | 886 |
| MS.gene005977.t1 | MTR_0368s0020 | 91.837 | 49 | 4 | 0 | 224 | 272 | 83 | 131 | 1.06e-24 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005977.t1 | AT1G48850 | 79.206 | 428 | 80 | 3 | 2 | 425 | 3 | 425 | 0.0 | 703 |
| MS.gene005977.t1 | AT1G48850 | 78.453 | 362 | 69 | 3 | 2 | 359 | 3 | 359 | 0.0 | 588 |
| MS.gene005977.t1 | AT1G48850 | 78.453 | 362 | 69 | 3 | 2 | 359 | 3 | 359 | 0.0 | 588 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATGTATCAGTCTCCTTTC+TGG | 0.119746 | 1.3:+66360653 | None:intergenic |
| CACTTCTTCTGTTTCTAATT+TGG | 0.135458 | 1.3:+66353628 | None:intergenic |
| ATCATCTTCTCTGCATATTC+AGG | 0.226636 | 1.3:+66357034 | None:intergenic |
| GCGAGATGTCATTAGCTTAT+AGG | 0.246076 | 1.3:-66360357 | MS.gene005977:CDS |
| GAGGGATCGGAGATTGGAAT+TGG | 0.269061 | 1.3:+66361803 | None:intergenic |
| TACATATGGGAACCACTTTC+GGG | 0.279701 | 1.3:-66361018 | MS.gene005977:CDS |
| CAATTTGGTAGTGGGTTTGC+AGG | 0.291322 | 1.3:-66355289 | MS.gene005977:intron |
| CACTTTCGGGTTACAACATA+TGG | 0.302913 | 1.3:-66361005 | MS.gene005977:CDS |
| CATGTATCAGTCTCCTTTCT+GGG | 0.318592 | 1.3:+66360654 | None:intergenic |
| TTCATTATTTCTCAGGGTCT+TGG | 0.321630 | 1.3:-66355385 | MS.gene005977:intron |
| GTACATATGGGAACCACTTT+CGG | 0.342196 | 1.3:-66361019 | MS.gene005977:CDS |
| TAAAATATTCTCAGGAGTTT+CGG | 0.342404 | 1.3:-66360631 | MS.gene005977:CDS |
| TTTCCTCTTTGATCAGTATT+GGG | 0.342486 | 1.3:+66360489 | None:intergenic |
| GCTTCAAGTTTGTCAAATAC+TGG | 0.357310 | 1.3:+66355358 | None:intergenic |
| ATTTCCTCTTTGATCAGTAT+TGG | 0.358775 | 1.3:+66360488 | None:intergenic |
| GTGTTCGCTCCCAGTCAAAA+AGG | 0.368874 | 1.3:+66355137 | None:intergenic |
| CCTTTCTGGGAGTTGTAATT+CGG | 0.369236 | 1.3:+66360667 | None:intergenic |
| CGGGCAGCTGGGAGTACATA+TGG | 0.371354 | 1.3:-66361032 | MS.gene005977:CDS |
| ACCATTGGAAGGGTTGCTTC+TGG | 0.385048 | 1.3:-66360167 | MS.gene005977:CDS |
| GAAATAATTAACATGAGAAT+AGG | 0.385678 | 1.3:-66354918 | MS.gene005977:CDS |
| AATTGAAAACCCTTAGTTGC+GGG | 0.393669 | 1.3:+66355307 | None:intergenic |
| TTCTCTGCATATTCAGGGTC+TGG | 0.396812 | 1.3:+66357040 | None:intergenic |
| TTTACAGAGATTCGGGCAGC+TGG | 0.397683 | 1.3:-66361044 | MS.gene005977:intron |
| CTCATGGTCAATTAAGTCTT+CGG | 0.397981 | 1.3:+66358282 | None:intergenic |
| CATGTTAATTATTTCTCCAT+TGG | 0.412777 | 1.3:+66354926 | None:intergenic |
| TATGTCATTGCCCGCAACTA+AGG | 0.414702 | 1.3:-66355317 | MS.gene005977:CDS |
| GGAGGAGAGGGATCGGAGAT+TGG | 0.418731 | 1.3:+66361797 | None:intergenic |
| GGTACTTGTGGACCAATTGA+TGG | 0.418936 | 1.3:-66353711 | MS.gene005977:CDS |
| GGAAGGAGTGAGGAAGGGTT+TGG | 0.419419 | 1.3:+66361857 | None:intergenic |
| AGGGATCGGAGATTGGAATT+GGG | 0.422790 | 1.3:+66361804 | None:intergenic |
| TCATGGTCAATTAAGTCTTC+GGG | 0.427778 | 1.3:+66358283 | None:intergenic |
| GTATGGTGTCAGATCAGTTC+AGG | 0.431140 | 1.3:-66360302 | MS.gene005977:intron |
| AAACCGCTCTGGTGGGATAC+AGG | 0.446252 | 1.3:-66355067 | MS.gene005977:intron |
| GGTAGAGGCCATGGTAGCTT+TGG | 0.452564 | 1.3:-66353732 | MS.gene005977:CDS |
| TGACAAACTTGAAGCTGAGT+TGG | 0.456051 | 1.3:-66355350 | MS.gene005977:CDS |
| AGGAAGAGAAAGAATCGGTT+CGG | 0.456406 | 1.3:+66361835 | None:intergenic |
| GCAACCTATGACATGAAGTA+TGG | 0.467320 | 1.3:-66360319 | MS.gene005977:CDS |
| GTGCATGTTGTGCCATCAAT+TGG | 0.469901 | 1.3:+66353699 | None:intergenic |
| GTTGTAATTCGGCTTTGACC+TGG | 0.476712 | 1.3:+66360678 | None:intergenic |
| CAGTATTGGGCACAAATACA+TGG | 0.482313 | 1.3:+66360502 | None:intergenic |
| TTGGGCACAAATACATGGAT+TGG | 0.483473 | 1.3:+66360507 | None:intergenic |
| GGTAGACGGAGGAGAGGGAT+CGG | 0.486339 | 1.3:+66361790 | None:intergenic |
| TGTTTCTAATTTGGCTGACA+AGG | 0.491775 | 1.3:+66353637 | None:intergenic |
| GGAAGAGAAAGAATCGGTTC+GGG | 0.494003 | 1.3:+66361836 | None:intergenic |
| GGTGTTGGATGTGTCATTGA+TGG | 0.495772 | 1.3:-66360966 | MS.gene005977:CDS |
| GATACATGTAAAATATTCTC+AGG | 0.496194 | 1.3:-66360639 | MS.gene005977:CDS |
| CCGAATTACAACTCCCAGAA+AGG | 0.500551 | 1.3:-66360667 | MS.gene005977:CDS |
| ATTGGAATTGGGAGAAAGGA+AGG | 0.503710 | 1.3:+66361815 | None:intergenic |
| AGGAGTGAGGAAGGGTTTGG+TGG | 0.507069 | 1.3:+66361860 | None:intergenic |
| GAATCTCATGGAGGAGGTGT+TGG | 0.513832 | 1.3:-66360981 | MS.gene005977:CDS |
| TGAGACACTGACACTTGATC+AGG | 0.515093 | 1.3:-66358263 | MS.gene005977:intron |
| AACTCCTATATTTAGGGTGG+TGG | 0.515942 | 1.3:-66360209 | MS.gene005977:intron |
| GGAGATTGGAATTGGGAGAA+AGG | 0.519246 | 1.3:+66361811 | None:intergenic |
| GGGCAGCTGGGAGTACATAT+GGG | 0.522096 | 1.3:-66361031 | MS.gene005977:CDS |
| CATGGTAGCTTTGGTACTTG+TGG | 0.522487 | 1.3:-66353723 | MS.gene005977:CDS |
| TCATCTTCTCTGCATATTCA+GGG | 0.523950 | 1.3:+66357035 | None:intergenic |
| GGCTGAGATACCAACCTCTC+GGG | 0.524544 | 1.3:+66354127 | None:intergenic |
| ACGGAGGTAGACGGAGGAGA+GGG | 0.527565 | 1.3:+66361785 | None:intergenic |
| TTCTTGTAAATCAAAGTTCG+CGG | 0.527748 | 1.3:+66353661 | None:intergenic |
| AAGGAAGGAAGAGAAAGAAT+CGG | 0.529630 | 1.3:+66361830 | None:intergenic |
| GACGGAGGTAGACGGAGGAG+AGG | 0.531416 | 1.3:+66361784 | None:intergenic |
| TTAATTGCAGGAGTTACTAC+AGG | 0.532875 | 1.3:-66360534 | MS.gene005977:intron |
| GTTCTTGTGTATAGGAGGCC+AGG | 0.534576 | 1.3:-66360696 | MS.gene005977:intron |
| TGTCATAGGTTGCATCCGCA+TGG | 0.536508 | 1.3:+66360329 | None:intergenic |
| GACACCATACTTCATGTCAT+AGG | 0.537696 | 1.3:+66360315 | None:intergenic |
| TCTTGTAAATCAAAGTTCGC+GGG | 0.540183 | 1.3:+66353662 | None:intergenic |
| ATGTCATTGCCCGCAACTAA+GGG | 0.549586 | 1.3:-66355316 | MS.gene005977:CDS |
| TCTTCTGCTAGGGAAACCAT+TGG | 0.551202 | 1.3:-66360182 | MS.gene005977:CDS |
| GGTTCGGGAAGGAGTGAGGA+AGG | 0.551542 | 1.3:+66361851 | None:intergenic |
| GTGGTGGTAGATCTTCTGCT+AGG | 0.562776 | 1.3:-66360193 | MS.gene005977:CDS |
| GAAACGAGGACGGAGGTAGA+CGG | 0.563398 | 1.3:+66361776 | None:intergenic |
| CCTGTGCACTTGAGAGACAT+AGG | 0.564384 | 1.3:+66358315 | None:intergenic |
| TGCAGCTGTACCCATGGTAG+AGG | 0.565756 | 1.3:-66353747 | MS.gene005977:intron |
| CTCTGAAGCTGATATGCAAG+AGG | 0.568295 | 1.3:-66360922 | MS.gene005977:CDS |
| AGCTACCATGGCCTCTACCA+TGG | 0.568585 | 1.3:+66353736 | None:intergenic |
| ACCAGAAGCAACCCTTCCAA+TGG | 0.569596 | 1.3:+66360166 | None:intergenic |
| CCTATGTCTCTCAAGTGCAC+AGG | 0.572163 | 1.3:-66358315 | MS.gene005977:CDS |
| CAACCTCTCGGGACAACACA+GGG | 0.572864 | 1.3:+66354138 | None:intergenic |
| CATATGTTGTAACCCGAAAG+TGG | 0.573615 | 1.3:+66361006 | None:intergenic |
| ACAAGAACAAACCGCTCTGG+TGG | 0.573699 | 1.3:-66355075 | MS.gene005977:CDS |
| ACAGGGATCATGACGACCAC+GGG | 0.574091 | 1.3:+66354155 | None:intergenic |
| TGCTAGGGAAACCATTGGAA+GGG | 0.574957 | 1.3:-66360177 | MS.gene005977:CDS |
| TGTACCCATGGTAGAGGCCA+TGG | 0.577435 | 1.3:-66353741 | MS.gene005977:CDS |
| GTCATAGGTTGCATCCGCAT+GGG | 0.577755 | 1.3:+66360330 | None:intergenic |
| CTGCTAGGGAAACCATTGGA+AGG | 0.577836 | 1.3:-66360178 | MS.gene005977:CDS |
| GTGAAAGGTGATTCTGTTGG+TGG | 0.578147 | 1.3:-66356989 | MS.gene005977:CDS |
| CAACAGAATCACCTTTCACT+CGG | 0.578783 | 1.3:+66356993 | None:intergenic |
| AGGACAAGAACAAACCGCTC+TGG | 0.581332 | 1.3:-66355078 | MS.gene005977:CDS |
| TGGTGGTAGATCTTCTGCTA+GGG | 0.582067 | 1.3:-66360192 | MS.gene005977:CDS |
| ACAACATATGGAGAATCTCA+TGG | 0.584104 | 1.3:-66360993 | MS.gene005977:CDS |
| TTATATAGATCAAAATGGAA+AGG | 0.585581 | 1.3:-66355103 | MS.gene005977:CDS |
| GATCAAGTGTCAGTGTCTCA+TGG | 0.586576 | 1.3:+66358266 | None:intergenic |
| ATTGATGCTGTCCGAGTGAA+AGG | 0.587265 | 1.3:-66357004 | MS.gene005977:CDS |
| GCTACCATGGCCTCTACCAT+GGG | 0.587349 | 1.3:+66353737 | None:intergenic |
| AATCAGGGTGGAATTACCAA+TGG | 0.587531 | 1.3:-66354942 | MS.gene005977:intron |
| TGCATATCAGCTTCAGAGAG+AGG | 0.589694 | 1.3:+66360927 | None:intergenic |
| TTACAGAGATTCGGGCAGCT+GGG | 0.592616 | 1.3:-66361043 | MS.gene005977:intron |
| GCTTCAGAGAGAGGGAGGCG+AGG | 0.596196 | 1.3:+66360936 | None:intergenic |
| GCATATCAGCTTCAGAGAGA+GGG | 0.596658 | 1.3:+66360928 | None:intergenic |
| GAAGCAATCTACAGTGACTC+GGG | 0.598566 | 1.3:-66354202 | MS.gene005977:CDS |
| GTGCCCAATACTGATCAAAG+AGG | 0.603337 | 1.3:-66360492 | MS.gene005977:CDS |
| CACAAGTACCAAAGCTACCA+TGG | 0.605561 | 1.3:+66353724 | None:intergenic |
| CACAGGGATCATGACGACCA+CGG | 0.605920 | 1.3:+66354154 | None:intergenic |
| ATATTCTCAGGAGTTTCGGA+AGG | 0.606596 | 1.3:-66360627 | MS.gene005977:intron |
| TGAACTTGCAGCTGTACCCA+TGG | 0.607274 | 1.3:-66353753 | MS.gene005977:intron |
| TATGGAGAATCTCATGGAGG+AGG | 0.607485 | 1.3:-66360987 | MS.gene005977:CDS |
| TAGGTTGCATCCGCATGGGA+AGG | 0.607769 | 1.3:+66360334 | None:intergenic |
| AGAAGCAATCTACAGTGACT+CGG | 0.610730 | 1.3:-66354203 | MS.gene005977:CDS |
| CCAACCTCTCGGGACAACAC+AGG | 0.611393 | 1.3:+66354137 | None:intergenic |
| AGCTTATAGGCCTTCCCATG+CGG | 0.616697 | 1.3:-66360344 | MS.gene005977:CDS |
| AATCGGTTCGGGAAGGAGTG+AGG | 0.619223 | 1.3:+66361847 | None:intergenic |
| GGTGGAGATCGAAGAAGCCA+TGG | 0.622096 | 1.3:+66361878 | None:intergenic |
| GTTCGGGAAGGAGTGAGGAA+GGG | 0.624145 | 1.3:+66361852 | None:intergenic |
| AAATTGAAAACCCTTAGTTG+CGG | 0.624146 | 1.3:+66355306 | None:intergenic |
| TCAGAGAGAGGGAGGCGAGG+AGG | 0.627324 | 1.3:+66360939 | None:intergenic |
| GAGAAAGAATCGGTTCGGGA+AGG | 0.628737 | 1.3:+66361840 | None:intergenic |
| TGCAAGAGGATCTTGACAGA+AGG | 0.632234 | 1.3:-66360908 | MS.gene005977:intron |
| CAAGAACAAACCGCTCTGGT+GGG | 0.632701 | 1.3:-66355074 | MS.gene005977:CDS |
| CTTACTGTAATAGTCGATGT+CGG | 0.643905 | 1.3:+66354888 | None:intergenic |
| TATCAGCTTCAGAGAGAGGG+AGG | 0.644707 | 1.3:+66360931 | None:intergenic |
| TAGATCAAAATGGAAAGGTG+AGG | 0.648351 | 1.3:-66355098 | MS.gene005977:CDS |
| CGAGTGAAAGGTGATTCTGT+TGG | 0.682023 | 1.3:-66356992 | MS.gene005977:CDS |
| GAAACAGAGCTCATAGCCCG+TGG | 0.694287 | 1.3:-66354171 | MS.gene005977:CDS |
| ACGAGGACGGAGGTAGACGG+AGG | 0.699102 | 1.3:+66361779 | None:intergenic |
| GTGTTGTAACATGCATTGTG+AGG | 0.723456 | 1.3:-66356967 | MS.gene005977:CDS |
| GATCCCTGTGTTGTCCCGAG+AGG | 0.731256 | 1.3:-66354141 | MS.gene005977:intron |
| ACATATGGAGAATCTCATGG+AGG | 0.763629 | 1.3:-66360990 | MS.gene005977:CDS |
| ATACCTGTATCCCACCAGAG+CGG | 0.816288 | 1.3:+66355064 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 66353619 | 66361898 | 66353619 | ID=MS.gene005977 |
| chr1.3 | mRNA | 66353619 | 66361898 | 66353619 | ID=MS.gene005977.t1;Parent=MS.gene005977 |
| chr1.3 | exon | 66361760 | 66361898 | 66361760 | ID=MS.gene005977.t1.exon1;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66361760 | 66361898 | 66361760 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66360909 | 66361059 | 66360909 | ID=MS.gene005977.t1.exon2;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66360909 | 66361059 | 66360909 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66360628 | 66360704 | 66360628 | ID=MS.gene005977.t1.exon3;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66360628 | 66360704 | 66360628 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66360488 | 66360546 | 66360488 | ID=MS.gene005977.t1.exon4;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66360488 | 66360546 | 66360488 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66360303 | 66360386 | 66360303 | ID=MS.gene005977.t1.exon5;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66360303 | 66360386 | 66360303 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66360124 | 66360216 | 66360124 | ID=MS.gene005977.t1.exon6;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66360124 | 66360216 | 66360124 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66358264 | 66358344 | 66358264 | ID=MS.gene005977.t1.exon7;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66358264 | 66358344 | 66358264 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66356955 | 66357086 | 66356955 | ID=MS.gene005977.t1.exon8;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66356955 | 66357086 | 66356955 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66355290 | 66355392 | 66355290 | ID=MS.gene005977.t1.exon9;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66355290 | 66355392 | 66355290 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66355068 | 66355162 | 66355068 | ID=MS.gene005977.t1.exon10;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66355068 | 66355162 | 66355068 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66354893 | 66354958 | 66354893 | ID=MS.gene005977.t1.exon11;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66354893 | 66354958 | 66354893 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66354142 | 66354226 | 66354142 | ID=MS.gene005977.t1.exon12;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66354142 | 66354226 | 66354142 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| chr1.3 | exon | 66353619 | 66353764 | 66353619 | ID=MS.gene005977.t1.exon13;Parent=MS.gene005977.t1 |
| chr1.3 | CDS | 66353619 | 66353764 | 66353619 | ID=cds.MS.gene005977.t1;Parent=MS.gene005977.t1 |
| Gene Sequence |
| Protein sequence |
