AlfalfaGEDB | MS.gene008401

M. sativa cultivar XinJiangDaYe / MS.gene008401

KEGG: K01520
GO: GO:0000287 GO:0003674 GO:0003824 GO:0004170 GO:0005488 GO:0006139 GO:0006220 GO:0006221 GO:0006226 GO:0006244 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009141 GO:0009143 GO:0009147 GO:0009149 GO:0009157 GO:0009162 GO:0009165 GO:0009166 GO:0009176 GO:0009177 GO:0009200 GO:0009204 GO:0009211 GO:0009213 GO:0009219 GO:0009221 GO:0009223 GO:0009262 GO:0009263 GO:0009264 GO:0009265 GO:0009394 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019692 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0043167 GO:0043169 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0046078 GO:0046080 GO:0046081 GO:0046385 GO:0046386 GO:0046434 GO:0046483 GO:0046700 GO:0046872 GO:0047429 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0072529 GO:0090407 GO:1901135 GO:1901136 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576
PFAM: PF00692.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008401.t1	RHN67997.1	98	150	3	0	1	150	38	187	1.00E-74	289.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008401.t1	Q9STG6	87.2	141	18	0	10	150	26	166	1.7e-65	250.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008401.t1	A0A072UXL1	98.0	150	3	0	1	150	1	150	7.5e-75	289.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene008401	MS.gene066960	PPI
MS.gene79799	MS.gene008401	PPI
MS.gene042871	MS.gene008401	PPI
MS.gene49838	MS.gene008401	PPI
MS.gene008401	MS.gene47284	PPI
MS.gene87898	MS.gene008401	PPI
MS.gene55626	MS.gene008401	PPI
MS.gene82109	MS.gene008401	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008401.t1	MTR_3g063110	98.000	150	3	0	1	150	1	150	1.92e-103	292

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008401.t1	AT3G46940	87.234	141	18	0	10	150	26	166	9.14e-87	251
MS.gene008401.t1	AT3G46940	87.234	141	18	0	10	150	67	207	9.43e-87	252

Find 45 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTGAAATTTCTGAGGTTT+TGG	0.210645	3.1:+53115039	MS.gene008401:CDS
GTTAGAGGTGAAGGTGGTTT+TGG	0.293840	3.1:+53115076	MS.gene008401:CDS
GATTTGGATTCTACTGTTAG+AGG	0.343621	3.1:+53115061	MS.gene008401:CDS
TTTCTTCACTCTGAGAAAAC+TGG	0.362791	3.1:-53114606	None:intergenic
ACACGAGCATAGGTTCCTTC+AGG	0.369361	3.1:-53114761	None:intergenic
TCCAAAACCTCAGAAATTTC+AGG	0.370463	3.1:-53115040	None:intergenic
AACCTTTGAATCGGCGGCGC+TGG	0.374420	3.1:-53114690	None:intergenic
TCAACAATGTCCAACTTAAA+CGG	0.388632	3.1:+53114572	None:intergenic
CTGATTACAGAGGACCTGTT+GGG	0.415838	3.1:+53114930	MS.gene008401:CDS
CATTCGATTGACGTTGGTGC+TGG	0.432456	3.1:+53114896	MS.gene008401:CDS
GGCGGAGAGAGGAGAGCCGC+GGG	0.455885	3.1:-53114654	None:intergenic
ATGAGAGCTTTGCCGCGAGC+AGG	0.461473	3.1:-53114713	None:intergenic
TGAAACGACGCCGTTTAAGT+TGG	0.465891	3.1:-53114582	None:intergenic
CGCCGAGATCGGGGTTGACA+TGG	0.473074	3.1:+53114870	MS.gene008401:CDS
TTCCATGTCAACCCCGATCT+CGG	0.481839	3.1:-53114872	None:intergenic
TTAAACAGTATAACCCCAAC+AGG	0.497604	3.1:-53114944	None:intergenic
CGCGGCTCTCCTCTCTCCGC+CGG	0.497937	3.1:+53114656	MS.gene008401:CDS
GGAAGGTATAACGGCTTTGT+CGG	0.505146	3.1:-53114633	None:intergenic
GATTCTACTGTTAGAGGTGA+AGG	0.505946	3.1:+53115067	MS.gene008401:CDS
CTCCAGCGCCGCCGATTCAA+AGG	0.509909	3.1:+53114688	MS.gene008401:CDS
CTCTCCATCGCCGTCCCTGA+AGG	0.513836	3.1:+53114746	MS.gene008401:CDS
GGCGCTGGAGAGATCGTATC+CGG	0.519854	3.1:-53114675	None:intergenic
CACGAGCATAGGTTCCTTCA+GGG	0.535140	3.1:-53114760	None:intergenic
GCGAGCAGGAACCTTTGAAT+CGG	0.540643	3.1:-53114699	None:intergenic
AGAGCCGCGGGAAGGTATAA+CGG	0.542038	3.1:-53114642	None:intergenic
ACGGCGATGGAGAGATCTGT+GGG	0.554559	3.1:-53114737	None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG	0.559917	3.1:-53114750	None:intergenic
TATTACTCCTGAAATTTCTG+AGG	0.568921	3.1:+53115033	MS.gene008401:CDS
TCTACTGTTAGAGGTGAAGG+TGG	0.578300	3.1:+53115070	MS.gene008401:CDS
GACGGCGATGGAGAGATCTG+TGG	0.578622	3.1:-53114738	None:intergenic
AGCAGGAACCTTTGAATCGG+CGG	0.581168	3.1:-53114696	None:intergenic
GTAATTACCAACACGAGCAT+AGG	0.588762	3.1:-53114771	None:intergenic
AGATCGTATCCGGCGGAGAG+AGG	0.592423	3.1:-53114665	None:intergenic
GAGAGAGGAGAGCCGCGGGA+AGG	0.593764	3.1:-53114650	None:intergenic
GAAGGAACCTATGCTCGTGT+TGG	0.606547	3.1:+53114764	MS.gene008401:CDS
CGGCGGAGAGAGGAGAGCCG+CGG	0.611864	3.1:-53114655	None:intergenic
GCTGATTACAGAGGACCTGT+TGG	0.614644	3.1:+53114929	MS.gene008401:CDS
TGGAAGCATTCGATTGACGT+TGG	0.614719	3.1:+53114890	MS.gene008401:CDS
AGCATAGGTTCCTTCAGGGA+CGG	0.629807	3.1:-53114756	None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG	0.632457	3.1:+53114701	MS.gene008401:CDS
TGATTACAGAGGACCTGTTG+GGG	0.654205	3.1:+53114931	MS.gene008401:CDS
AAAGCCGTTATACCTTCCCG+CGG	0.736048	3.1:+53114638	MS.gene008401:CDS
GTGATTGATGCTGATTACAG+AGG	0.740502	3.1:+53114920	MS.gene008401:CDS
GCTGGAGAGATCGTATCCGG+CGG	0.743244	3.1:-53114672	None:intergenic
CGGCGATGGAGAGATCTGTG+GGG	0.756952	3.1:-53114736	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAACCCTGAAATTCAAAAT+TGG	-	chr3.1:53114811-53114830	None:intergenic	25.0%
!	AAACCCTGAAATTCAAAATT+GGG	-	chr3.1:53114810-53114829	None:intergenic	25.0%
!	TGAAATTCAAAATTGGGAAA+GGG	-	chr3.1:53114804-53114823	None:intergenic	25.0%
!!	TTTCCCAATTTTGAATTTCA+GGG	+	chr3.1:53114804-53114823	MS.gene008401:intron	25.0%
!!!	AATTTCAGGGTTTTGAATTT+AGG	+	chr3.1:53114817-53114836	MS.gene008401:intron	25.0%
!!!	ATTTCAGGGTTTTGAATTTA+GGG	+	chr3.1:53114818-53114837	MS.gene008401:intron	25.0%
	CTGAAATTCAAAATTGGGAA+AGG	-	chr3.1:53114805-53114824	None:intergenic	30.0%
	GAAATTCAAAATTGGGAAAG+GGG	-	chr3.1:53114803-53114822	None:intergenic	30.0%
	TATTACTCCTGAAATTTCTG+AGG	+	chr3.1:53115033-53115052	MS.gene008401:CDS	30.0%
!	CTTTCCCAATTTTGAATTTC+AGG	+	chr3.1:53114803-53114822	MS.gene008401:intron	30.0%
!!!	AATTTCTGAGGTTTTGGATT+TGG	+	chr3.1:53115045-53115064	MS.gene008401:CDS	30.0%
!!!	GTTGATTTTGAAGTGAAAGT+TGG	+	chr3.1:53114977-53114996	MS.gene008401:CDS	30.0%
	GATTTGGATTCTACTGTTAG+AGG	+	chr3.1:53115061-53115080	MS.gene008401:CDS	35.0%
	TCCAAAACCTCAGAAATTTC+AGG	-	chr3.1:53115043-53115062	None:intergenic	35.0%
	TTAAACAGTATAACCCCAAC+AGG	-	chr3.1:53114947-53114966	None:intergenic	35.0%
!	TTTCTTCACTCTGAGAAAAC+TGG	-	chr3.1:53114609-53114628	None:intergenic	35.0%
!!!	TCCTGAAATTTCTGAGGTTT+TGG	+	chr3.1:53115039-53115058	MS.gene008401:CDS	35.0%
	GATTCTACTGTTAGAGGTGA+AGG	+	chr3.1:53115067-53115086	MS.gene008401:CDS	40.0%
	GTAATTACCAACACGAGCAT+AGG	-	chr3.1:53114774-53114793	None:intergenic	40.0%
!	GTGATTGATGCTGATTACAG+AGG	+	chr3.1:53114920-53114939	MS.gene008401:CDS	40.0%
!!!	AAGGTGGTTTTGGATCTACT+GGG	+	chr3.1:53115086-53115105	MS.gene008401:CDS	40.0%
	CTGATTACAGAGGACCTGTT+GGG	+	chr3.1:53114930-53114949	MS.gene008401:CDS	45.0%
	TCTACTGTTAGAGGTGAAGG+TGG	+	chr3.1:53115070-53115089	MS.gene008401:CDS	45.0%
	TGAAACGACGCCGTTTAAGT+TGG	-	chr3.1:53114585-53114604	None:intergenic	45.0%
	TGATTACAGAGGACCTGTTG+GGG	+	chr3.1:53114931-53114950	MS.gene008401:CDS	45.0%
!	GGAAGGTATAACGGCTTTGT+CGG	-	chr3.1:53114636-53114655	None:intergenic	45.0%
!	GTTAGAGGTGAAGGTGGTTT+TGG	+	chr3.1:53115076-53115095	MS.gene008401:CDS	45.0%
!!	TGGAAGCATTCGATTGACGT+TGG	+	chr3.1:53114890-53114909	MS.gene008401:CDS	45.0%
!!!	AGGTGGTTTTGGATCTACTG+GGG	+	chr3.1:53115087-53115106	MS.gene008401:CDS	45.0%
!!!	GAAGGTGGTTTTGGATCTAC+TGG	+	chr3.1:53115085-53115104	MS.gene008401:CDS	45.0%
	AAAGCCGTTATACCTTCCCG+CGG	+	chr3.1:53114638-53114657	MS.gene008401:CDS	50.0%
	ACACGAGCATAGGTTCCTTC+AGG	-	chr3.1:53114764-53114783	None:intergenic	50.0%
	AGCAGGAACCTTTGAATCGG+CGG	-	chr3.1:53114699-53114718	None:intergenic	50.0%
	AGCATAGGTTCCTTCAGGGA+CGG	-	chr3.1:53114759-53114778	None:intergenic	50.0%
	CACGAGCATAGGTTCCTTCA+GGG	-	chr3.1:53114763-53114782	None:intergenic	50.0%
	CATTCGATTGACGTTGGTGC+TGG	+	chr3.1:53114896-53114915	MS.gene008401:CDS	50.0%
	GAAGGAACCTATGCTCGTGT+TGG	+	chr3.1:53114764-53114783	MS.gene008401:CDS	50.0%
	GATTCAAAGGTTCCTGCTCG+CGG	+	chr3.1:53114701-53114720	MS.gene008401:CDS	50.0%
	GCGAGCAGGAACCTTTGAAT+CGG	-	chr3.1:53114702-53114721	None:intergenic	50.0%
	GCTGATTACAGAGGACCTGT+TGG	+	chr3.1:53114929-53114948	MS.gene008401:CDS	50.0%
	TTCCATGTCAACCCCGATCT+CGG	-	chr3.1:53114875-53114894	None:intergenic	50.0%
!!	ATTTTTGCAGCGCCGAGATC+GGG	+	chr3.1:53114860-53114879	MS.gene008401:intron	50.0%
!!	GATTTTTGCAGCGCCGAGAT+CGG	+	chr3.1:53114859-53114878	MS.gene008401:intron	50.0%
	ACGGCGATGGAGAGATCTGT+GGG	-	chr3.1:53114740-53114759	None:intergenic	55.0%
!	AGAGCCGCGGGAAGGTATAA+CGG	-	chr3.1:53114645-53114664	None:intergenic	55.0%
!!	TTTTTGCAGCGCCGAGATCG+GGG	+	chr3.1:53114861-53114880	MS.gene008401:intron	55.0%
	AACCTTTGAATCGGCGGCGC+TGG	-	chr3.1:53114693-53114712	None:intergenic	60.0%
	AGATCGTATCCGGCGGAGAG+AGG	-	chr3.1:53114668-53114687	None:intergenic	60.0%
	ATGAGAGCTTTGCCGCGAGC+AGG	-	chr3.1:53114716-53114735	None:intergenic	60.0%
	CGGCGATGGAGAGATCTGTG+GGG	-	chr3.1:53114739-53114758	None:intergenic	60.0%
	GACGGCGATGGAGAGATCTG+TGG	-	chr3.1:53114741-53114760	None:intergenic	60.0%
	GCTGGAGAGATCGTATCCGG+CGG	-	chr3.1:53114675-53114694	None:intergenic	60.0%
!	GGCGCTGGAGAGATCGTATC+CGG	-	chr3.1:53114678-53114697	None:intergenic	60.0%
	CGCCGAGATCGGGGTTGACA+TGG	+	chr3.1:53114870-53114889	MS.gene008401:CDS	65.0%
	CTCTCCATCGCCGTCCCTGA+AGG	+	chr3.1:53114746-53114765	MS.gene008401:CDS	65.0%
	GGTTCCTTCAGGGACGGCGA+TGG	-	chr3.1:53114753-53114772	None:intergenic	65.0%
!!	CTCCAGCGCCGCCGATTCAA+AGG	+	chr3.1:53114688-53114707	MS.gene008401:CDS	65.0%
	GAGAGAGGAGAGCCGCGGGA+AGG	-	chr3.1:53114653-53114672	None:intergenic	70.0%
	CGCGGCTCTCCTCTCTCCGC+CGG	+	chr3.1:53114656-53114675	MS.gene008401:CDS	75.0%
	CGGCGGAGAGAGGAGAGCCG+CGG	-	chr3.1:53114658-53114677	None:intergenic	75.0%
	GGCGGAGAGAGGAGAGCCGC+GGG	-	chr3.1:53114657-53114676	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	53114578	53115114	53114578	ID=MS.gene008401
chr3.1	mRNA	53114578	53115114	53114578	ID=MS.gene008401.t1;Parent=MS.gene008401
chr3.1	exon	53114578	53114785	53114578	ID=MS.gene008401.t1.exon1;Parent=MS.gene008401.t1
chr3.1	CDS	53114578	53114785	53114578	ID=cds.MS.gene008401.t1;Parent=MS.gene008401.t1
chr3.1	exon	53114870	53115114	53114870	ID=MS.gene008401.t1.exon2;Parent=MS.gene008401.t1
chr3.1	CDS	53114870	53115114	53114870	ID=cds.MS.gene008401.t1;Parent=MS.gene008401.t1

Gene Sequence

>MS.gene008401

ATGTCCAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCAGAGTGAAGAAACTCTCCGACAAAGCCGTTATACCTTCCCGCGGCTCTCCTCTCTCCGCCGGATACGATCTCTCCAGCGCCGCCGATTCAAAGGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGGTAATTACTGTTTTCCCCTTTCCCAATTTTGAATTTCAGGGTTTTGAATTTAGGGTTTTTGATTGAATTGAATGATTTTTGCAGCGCCGAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCAGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCTGAAATTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA

Protein sequence

>MS.gene008401.t1

MSNLNGVVSSSFLRVKKLSDKAVIPSRGSPLSAGYDLSSAADSKVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEISEVLDLDSTVRGEGGFGSTGV