Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008720.t1 | XP_003612356.1 | 99.7 | 393 | 1 | 0 | 1 | 393 | 1 | 393 | 5.80E-226 | 793.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008720.t1 | Q42807 | 91.5 | 390 | 31 | 1 | 1 | 390 | 1 | 388 | 2.5e-209 | 729.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008720.t1 | Q4U601 | 99.7 | 393 | 1 | 0 | 1 | 393 | 1 | 393 | 4.2e-226 | 793.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31844 | MS.gene008720 | PPI |
| MS.gene054155 | MS.gene008720 | PPI |
| MS.gene32324 | MS.gene008720 | PPI |
| MS.gene008720 | MS.gene026689 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008720.t1 | MTR_5g024130 | 99.746 | 393 | 1 | 0 | 1 | 393 | 1 | 393 | 0.0 | 817 |
| MS.gene008720.t1 | MTR_4g087440 | 98.728 | 393 | 5 | 0 | 1 | 393 | 1 | 393 | 0.0 | 808 |
| MS.gene008720.t1 | MTR_1g029150 | 72.271 | 339 | 90 | 2 | 59 | 393 | 52 | 390 | 0.0 | 526 |
| MS.gene008720.t1 | MTR_3g090100 | 64.489 | 352 | 113 | 4 | 46 | 393 | 23 | 366 | 5.54e-169 | 477 |
| MS.gene008720.t1 | MTR_3g089025 | 65.782 | 339 | 113 | 2 | 57 | 393 | 32 | 369 | 1.50e-168 | 476 |
| MS.gene008720.t1 | MTR_3g090020 | 64.000 | 350 | 118 | 3 | 46 | 393 | 23 | 366 | 2.37e-166 | 470 |
| MS.gene008720.t1 | MTR_3g090010 | 65.970 | 335 | 111 | 2 | 59 | 391 | 35 | 368 | 5.22e-165 | 467 |
| MS.gene008720.t1 | MTR_3g090110 | 40.171 | 117 | 47 | 1 | 237 | 353 | 2 | 95 | 7.04e-22 | 90.1 |
| MS.gene008720.t1 | MTR_3g089980 | 47.423 | 97 | 38 | 1 | 257 | 353 | 7 | 90 | 1.20e-21 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008720.t1 | AT2G43710 | 80.299 | 401 | 71 | 2 | 1 | 393 | 1 | 401 | 0.0 | 674 |
| MS.gene008720.t1 | AT2G43710 | 80.050 | 401 | 72 | 2 | 1 | 393 | 1 | 401 | 0.0 | 672 |
| MS.gene008720.t1 | AT3G02630 | 76.786 | 392 | 90 | 1 | 1 | 391 | 3 | 394 | 0.0 | 639 |
| MS.gene008720.t1 | AT5G16240 | 77.778 | 360 | 77 | 2 | 33 | 391 | 35 | 392 | 0.0 | 597 |
| MS.gene008720.t1 | AT3G02610 | 68.750 | 400 | 110 | 6 | 3 | 393 | 20 | 413 | 0.0 | 551 |
| MS.gene008720.t1 | AT3G02610 | 68.750 | 400 | 110 | 6 | 3 | 393 | 18 | 411 | 0.0 | 551 |
| MS.gene008720.t1 | AT5G16230 | 67.895 | 380 | 118 | 3 | 18 | 393 | 22 | 401 | 0.0 | 538 |
| MS.gene008720.t1 | AT3G02620 | 68.338 | 379 | 108 | 4 | 21 | 393 | 34 | 406 | 0.0 | 536 |
| MS.gene008720.t1 | AT3G02620 | 68.338 | 379 | 108 | 4 | 21 | 393 | 31 | 403 | 0.0 | 536 |
| MS.gene008720.t1 | AT1G43800 | 65.274 | 383 | 126 | 4 | 14 | 391 | 9 | 389 | 0.0 | 518 |
| MS.gene008720.t1 | AT5G16230 | 74.154 | 325 | 80 | 3 | 73 | 393 | 1 | 325 | 2.78e-180 | 504 |
| MS.gene008720.t1 | AT3G02620 | 73.394 | 327 | 81 | 3 | 73 | 393 | 1 | 327 | 3.47e-179 | 501 |
Find 129 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGATATACTTGAGTTTC+TGG | 0.154020 | 4.4:-25279660 | MS.gene008720:CDS |
| ATTTGAAAATTTCAATCTTC+TGG | 0.154979 | 4.4:+25285930 | None:intergenic |
| GCCCTACTCCTTGGGCTATT+TGG | 0.176215 | 4.4:-25285649 | MS.gene008720:CDS |
| TCCTCCAGCCTTCTGATTCT+TGG | 0.181095 | 4.4:+25279557 | None:intergenic |
| AACACTTCTCTACAGGTTTA+AGG | 0.197232 | 4.4:+25285870 | None:intergenic |
| GGTGGAAGCCATGACGAATT+TGG | 0.227957 | 4.4:+25287243 | None:intergenic |
| GCCGACATAACCGGACTTTC+AGG | 0.282345 | 4.4:-25279620 | MS.gene008720:CDS |
| CATTCAGTTGGATTCATGAT+AGG | 0.310680 | 4.4:-25279496 | MS.gene008720:CDS |
| TACTTGAACATGCACTTCTC+TGG | 0.313192 | 4.4:+25285970 | None:intergenic |
| CCAAACTATGCTTAATACTT+TGG | 0.323055 | 4.4:-25285699 | MS.gene008720:CDS |
| AGGTTTAAGGTGAGTCAATA+AGG | 0.334045 | 4.4:+25285883 | None:intergenic |
| AGATGATTACTTTGTTGTTC+TGG | 0.344575 | 4.4:-25285759 | MS.gene008720:CDS |
| TTAAGCATAGTTTGGTAAGT+AGG | 0.345744 | 4.4:+25285707 | None:intergenic |
| TTTCAAATCGTTGGAGGGTT+GGG | 0.350638 | 4.4:-25285915 | MS.gene008720:CDS |
| CCAAAGTATTAAGCATAGTT+TGG | 0.352890 | 4.4:+25285699 | None:intergenic |
| AATCCAACTGAATGCAAGTT+TGG | 0.355409 | 4.4:+25279505 | None:intergenic |
| TTTGAAAATTTCAATCTTCT+GGG | 0.358302 | 4.4:+25285931 | None:intergenic |
| GTGGAAGCCATGACGAATTT+GGG | 0.375597 | 4.4:+25287244 | None:intergenic |
| TGTTGTTCTGGTTGGAGATA+TGG | 0.377751 | 4.4:-25285747 | MS.gene008720:CDS |
| CTGGTGCCAGCCCTACTCCT+TGG | 0.386884 | 4.4:-25285658 | MS.gene008720:CDS |
| AAAGATAGTGGAAAAGCTAT+TGG | 0.403395 | 4.4:-25279840 | MS.gene008720:CDS |
| CTCCTTCTCTCTCCCTCAAA+TGG | 0.406762 | 4.4:-25287281 | MS.gene008720:CDS |
| CTGCCCGATCCTTCCTCAGA+TGG | 0.416238 | 4.4:-25285830 | MS.gene008720:CDS |
| ATGGCTTCCACCCTCCGTTC+TGG | 0.424747 | 4.4:-25287232 | MS.gene008720:CDS |
| CATCCAAACTTGCATTCAGT+TGG | 0.428488 | 4.4:-25279508 | MS.gene008720:CDS |
| AGAACAACAAAGTAATCATC+TGG | 0.438790 | 4.4:+25285761 | None:intergenic |
| GATATACTTGAGTTTCTGGT+TGG | 0.441237 | 4.4:-25279656 | MS.gene008720:CDS |
| TGGAAGGTGGCCGACATAAC+CGG | 0.441892 | 4.4:-25279629 | MS.gene008720:CDS |
| GGTTGCCAACACTTCTCTAC+AGG | 0.448685 | 4.4:+25285863 | None:intergenic |
| TGGTGCCAGCCCTACTCCTT+GGG | 0.450462 | 4.4:-25285657 | MS.gene008720:CDS |
| TTACCATTCCAGAGCCAATC+AGG | 0.453929 | 4.4:+25285519 | None:intergenic |
| AATAAGTATCTCTACTTGTC+CGG | 0.454382 | 4.4:-25285581 | MS.gene008720:CDS |
| TGTGTTTCCATGCGATATAA+AGG | 0.456327 | 4.4:+25279955 | None:intergenic |
| CAGCAGAATAGTTATCAAAC+AGG | 0.457192 | 4.4:+25279720 | None:intergenic |
| ATATACTTGAGTTTCTGGTT+GGG | 0.460993 | 4.4:-25279655 | MS.gene008720:CDS |
| GCCGCGTTCCTTAGCAAGCC+TGG | 0.462444 | 4.4:+25279931 | None:intergenic |
| AGAAGGAGGATGTTTGTGTA+GGG | 0.463532 | 4.4:+25287296 | None:intergenic |
| TCCAGCCTTCTGATTCTTGG+TGG | 0.465016 | 4.4:+25279560 | None:intergenic |
| TCGTCCCTCACCTGAAAGTC+CGG | 0.465890 | 4.4:+25279610 | None:intergenic |
| GATGGAGTTCGTGATGAAAC+TGG | 0.466056 | 4.4:-25285677 | MS.gene008720:CDS |
| TCTGCTGTTGCCCAGCGCAT+TGG | 0.472536 | 4.4:-25279704 | MS.gene008720:CDS |
| GGAACGCGGCGATTTGAAGT+TGG | 0.473114 | 4.4:-25279918 | MS.gene008720:CDS |
| TCCAAATAGCCCAAGGAGTA+GGG | 0.477229 | 4.4:+25285648 | None:intergenic |
| CAGTGTAGACTCCAATGCGC+TGG | 0.478141 | 4.4:+25279693 | None:intergenic |
| GTGTAGATAAAGCCAAGGTA+AGG | 0.480370 | 4.4:+25279998 | None:intergenic |
| GTTTCTGGTTGGGAGGTGGA+AGG | 0.480811 | 4.4:-25279645 | MS.gene008720:CDS |
| TACTTGAGTTTCTGGTTGGG+AGG | 0.483685 | 4.4:-25279652 | MS.gene008720:CDS |
| TATCGCATGGAAACACAGCC+AGG | 0.484282 | 4.4:-25279949 | MS.gene008720:CDS |
| TATCAAACAGGTTGTCATCT+CGG | 0.485495 | 4.4:+25279732 | None:intergenic |
| AGAGGTTGAAAATATTAAGA+AGG | 0.489403 | 4.4:-25286005 | MS.gene008720:CDS |
| AAGGCACAGGAGTATGTTTG+TGG | 0.492271 | 4.4:-25279587 | MS.gene008720:CDS |
| GAGAAGGAGGATGTTTGTGT+AGG | 0.492943 | 4.4:+25287295 | None:intergenic |
| ATTCAGTACCTGATTGGCTC+TGG | 0.492964 | 4.4:-25285527 | MS.gene008720:CDS |
| ATTTGAGACTGGCCATTTGA+GGG | 0.496342 | 4.4:+25287269 | None:intergenic |
| CTCCTTGGGCTATTTGGACA+AGG | 0.500836 | 4.4:-25285643 | MS.gene008720:CDS |
| GATTTGAGACTGGCCATTTG+AGG | 0.502339 | 4.4:+25287268 | None:intergenic |
| AAAGCAATAACTGTTCCATC+AGG | 0.503808 | 4.4:+25279809 | None:intergenic |
| TTGAGTTTCTGGTTGGGAGG+TGG | 0.503890 | 4.4:-25279649 | MS.gene008720:CDS |
| AAGACAATTCAGTACCTGAT+TGG | 0.503895 | 4.4:-25285533 | MS.gene008720:CDS |
| GACTGCTTACACAAAGATAG+TGG | 0.506240 | 4.4:-25279852 | MS.gene008720:CDS |
| CCATCATACATCAGGTGTGC+TGG | 0.507354 | 4.4:+25279755 | None:intergenic |
| ATTCAGTTGGATTCATGATA+GGG | 0.509293 | 4.4:-25279495 | MS.gene008720:CDS |
| ATCGTTGGAGGGTTGGGCTG+AGG | 0.511095 | 4.4:-25285909 | MS.gene008720:CDS |
| CATCTCGGCCATCATACATC+AGG | 0.513791 | 4.4:+25279747 | None:intergenic |
| ACTATGCTTAATACTTTGGA+TGG | 0.522020 | 4.4:-25285695 | MS.gene008720:CDS |
| GGGCTATTTGGACAAGGGCA+TGG | 0.524428 | 4.4:-25285637 | MS.gene008720:CDS |
| CTACACTTCATTTCAAGAGA+GGG | 0.526696 | 4.4:-25279981 | MS.gene008720:CDS |
| AATCCATCTGAGGAAGGATC+GGG | 0.533112 | 4.4:+25285827 | None:intergenic |
| ACTTGAACATGCACTTCTCT+GGG | 0.540588 | 4.4:+25285971 | None:intergenic |
| GCCACCAAGAATCAGAAGGC+TGG | 0.541195 | 4.4:-25279561 | MS.gene008720:CDS |
| GATTACTTTGTTGTTCTGGT+TGG | 0.542691 | 4.4:-25285755 | MS.gene008720:CDS |
| AATAGCCCAAGGAGTAGGGC+TGG | 0.546034 | 4.4:+25285652 | None:intergenic |
| AGGTGAGGGACGAAAGGCAC+AGG | 0.547789 | 4.4:-25279600 | MS.gene008720:CDS |
| GAATTTGGGAGATTTGAGAC+TGG | 0.549768 | 4.4:+25287258 | None:intergenic |
| GAATCCATCTGAGGAAGGAT+CGG | 0.550340 | 4.4:+25285826 | None:intergenic |
| TTGAAAATTTCAATCTTCTG+GGG | 0.552491 | 4.4:+25285932 | None:intergenic |
| ACCTGAAAGTCCGGTTATGT+CGG | 0.554587 | 4.4:+25279619 | None:intergenic |
| GTCCAAATAGCCCAAGGAGT+AGG | 0.558680 | 4.4:+25285647 | None:intergenic |
| TCTGGTTGGGAGGTGGAAGG+TGG | 0.560034 | 4.4:-25279642 | MS.gene008720:CDS |
| AGGGCGACCTTTATATCGCA+TGG | 0.560815 | 4.4:-25279962 | MS.gene008720:CDS |
| AAAATTTCAATCTTCTGGGG+AGG | 0.561159 | 4.4:+25285935 | None:intergenic |
| AGAGAAGTGTTGGCAACCAC+AGG | 0.562702 | 4.4:-25285858 | MS.gene008720:CDS |
| TTGAAGTTGGCTCAGATCTG+TGG | 0.563389 | 4.4:-25279905 | MS.gene008720:CDS |
| GTACCTGATTGGCTCTGGAA+TGG | 0.564475 | 4.4:-25285522 | MS.gene008720:intron |
| AGTGTAGACTCCAATGCGCT+GGG | 0.573443 | 4.4:+25279694 | None:intergenic |
| AAGTGAAGGAACTGAGAGAG+AGG | 0.573671 | 4.4:-25285796 | MS.gene008720:CDS |
| AGGCACAGGAGTATGTTTGT+GGG | 0.574102 | 4.4:-25279586 | MS.gene008720:CDS |
| AAGTATATCAGCATAGTCCT+TGG | 0.575108 | 4.4:+25279670 | None:intergenic |
| TTAAACCTGTAGAGAAGTGT+TGG | 0.579690 | 4.4:-25285868 | MS.gene008720:CDS |
| TCTACACTTCATTTCAAGAG+AGG | 0.581234 | 4.4:-25279982 | MS.gene008720:CDS |
| TTGTCTCATATCAACACGGC+CGG | 0.584046 | 4.4:+25285562 | None:intergenic |
| CTCATGGCGTTTCTCATCAG+AGG | 0.584747 | 4.4:+25279874 | None:intergenic |
| GAGGATGTTTGTGTAGGGAA+AGG | 0.586561 | 4.4:+25287301 | None:intergenic |
| GGCCATTTGAGGGAGAGAGA+AGG | 0.589016 | 4.4:+25287279 | None:intergenic |
| CATTTGAGGGAGAGAGAAGG+AGG | 0.590783 | 4.4:+25287282 | None:intergenic |
| TCTTCTGGGGAGGCATAGAG+TGG | 0.595890 | 4.4:+25285945 | None:intergenic |
| GAGAGCTTCAGCAAGAGCAA+AGG | 0.595934 | 4.4:-25279534 | MS.gene008720:CDS |
| AAACACAGCCAGGCTTGCTA+AGG | 0.597013 | 4.4:-25279939 | MS.gene008720:CDS |
| TGTAGATAAAGCCAAGGTAA+GGG | 0.598444 | 4.4:+25279999 | None:intergenic |
| CAAATCTCCCAAATTCGTCA+TGG | 0.598894 | 4.4:-25287251 | MS.gene008720:CDS |
| AATTTGATGGATAGGATCCT+CGG | 0.609645 | 4.4:-25280033 | MS.gene008720:intron |
| ACTTTCAGGTGAGGGACGAA+AGG | 0.610348 | 4.4:-25279606 | MS.gene008720:CDS |
| CCAGCACACCTGATGTATGA+TGG | 0.611997 | 4.4:-25279755 | MS.gene008720:CDS |
| CTATTGGAGATTGATCCTGA+TGG | 0.612852 | 4.4:-25279824 | MS.gene008720:CDS |
| GCCCTTGTCCAAATAGCCCA+AGG | 0.616169 | 4.4:+25285641 | None:intergenic |
| TCCTTGGGCTATTTGGACAA+GGG | 0.616511 | 4.4:-25285642 | MS.gene008720:CDS |
| TCTTCGAATCCATCTGAGGA+AGG | 0.617698 | 4.4:+25285821 | None:intergenic |
| GCCAGGCTTGCTAAGGAACG+CGG | 0.621905 | 4.4:-25279932 | MS.gene008720:CDS |
| AGTGAAGGAACTGAGAGAGA+GGG | 0.623958 | 4.4:-25285795 | MS.gene008720:CDS |
| ACTGAGAATAGCCCTTACCT+TGG | 0.627826 | 4.4:-25280010 | MS.gene008720:CDS |
| TGACTTGGAACCAGAACGGA+GGG | 0.629360 | 4.4:+25287222 | None:intergenic |
| ATGAAGTGTAGATAAAGCCA+AGG | 0.630791 | 4.4:+25279993 | None:intergenic |
| TTGCTCTTCGAATCCATCTG+AGG | 0.631251 | 4.4:+25285817 | None:intergenic |
| CAATTTGTCTCATATCAACA+CGG | 0.633578 | 4.4:+25285558 | None:intergenic |
| TCTTTGTGTAAGCAGTCTCA+TGG | 0.635579 | 4.4:+25279858 | None:intergenic |
| CTTGGAACCAGAACGGAGGG+TGG | 0.638218 | 4.4:+25287225 | None:intergenic |
| TGGATTCGAAGAGCAAGTGA+AGG | 0.638840 | 4.4:-25285810 | MS.gene008720:CDS |
| CATTGGAGTCTACACTGCCA+AGG | 0.641019 | 4.4:-25279687 | MS.gene008720:CDS |
| GGTTGCCACCAAGAATCAGA+AGG | 0.645168 | 4.4:-25279565 | MS.gene008720:CDS |
| ATAACCGGACTTTCAGGTGA+GGG | 0.664241 | 4.4:-25279614 | MS.gene008720:CDS |
| ACCAAGAATCAGAAGGCTGG+AGG | 0.675099 | 4.4:-25279558 | MS.gene008720:CDS |
| ATACTGACTTGGAACCAGAA+CGG | 0.677723 | 4.4:+25287218 | None:intergenic |
| TAAGGGCTATTCTCAGTCCG+AGG | 0.684437 | 4.4:+25280016 | None:intergenic |
| CATAACCGGACTTTCAGGTG+AGG | 0.689606 | 4.4:-25279615 | MS.gene008720:CDS |
| CTTCTGGGGAGGCATAGAGT+GGG | 0.692583 | 4.4:+25285946 | None:intergenic |
| TGAACATGCACTTCTCTGGG+AGG | 0.699562 | 4.4:+25285974 | None:intergenic |
| ACAGCTGAAGAAAATAGACA+TGG | 0.709949 | 4.4:-25285614 | MS.gene008720:CDS |
| GGATCGGGCAGAAAATCCTG+TGG | 0.717346 | 4.4:+25285842 | None:intergenic |
| CTGACTTGGAACCAGAACGG+AGG | 0.718153 | 4.4:+25287221 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 25279481 | 25287341 | 25279481 | ID=MS.gene008720 |
| chr4.4 | mRNA | 25279481 | 25287341 | 25279481 | ID=MS.gene008720.t1;Parent=MS.gene008720 |
| chr4.4 | exon | 25287226 | 25287341 | 25287226 | ID=MS.gene008720.t1.exon1;Parent=MS.gene008720.t1 |
| chr4.4 | CDS | 25287226 | 25287341 | 25287226 | ID=cds.MS.gene008720.t1;Parent=MS.gene008720.t1 |
| chr4.4 | exon | 25285523 | 25286027 | 25285523 | ID=MS.gene008720.t1.exon2;Parent=MS.gene008720.t1 |
| chr4.4 | CDS | 25285523 | 25286027 | 25285523 | ID=cds.MS.gene008720.t1;Parent=MS.gene008720.t1 |
| chr4.4 | exon | 25279481 | 25280041 | 25279481 | ID=MS.gene008720.t1.exon3;Parent=MS.gene008720.t1 |
| chr4.4 | CDS | 25279481 | 25280041 | 25279481 | ID=cds.MS.gene008720.t1;Parent=MS.gene008720.t1 |
| Gene Sequence |
| Protein sequence |
