AlfalfaGEDB | MS.gene009347

M. sativa cultivar XinJiangDaYe / MS.gene009347

KEGG: K17867
GO: -
PFAM: PF00226.34 PF05207.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009347.t1	XP_013458798.1	91.6	178	15	0	1	178	1	178	4.30E-83	317.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009347.t1	Q54CI5	28.6	182	90	4	10	176	14	170	3.2e-10	66.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009347.t1	A0A072V3W3	91.6	178	15	0	1	178	1	178	3.1e-83	317.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene009347	MS.gene028111	PPI
MS.gene009347	MS.gene83474	PPI
MS.gene009347	MS.gene72809	PPI
MS.gene009347	MS.gene82121	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009347.t1	MTR_3g006810	91.573	178	15	0	1	178	1	178	4.01e-118	332
MS.gene009347.t1	MTR_6g003930	90.449	178	17	0	1	178	1	178	1.36e-116	328
MS.gene009347.t1	MTR_6g003930	90.449	178	17	0	1	178	73	250	3.80e-115	327
MS.gene009347.t1	MTR_4g074580	78.947	133	27	1	1	132	1	133	1.08e-69	207

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009347.t1	AT4G10130	50.000	176	81	2	1	176	1	169	1.58e-57	178
MS.gene009347.t1	AT4G10130	50.000	176	81	2	1	176	1	169	1.58e-57	178
MS.gene009347.t1	AT4G10130	50.000	176	81	2	1	176	1	169	1.58e-57	178
MS.gene009347.t1	AT4G10130	50.000	176	81	2	1	176	1	169	1.58e-57	178
MS.gene009347.t1	AT4G10130	50.000	176	81	2	1	176	1	169	1.58e-57	178

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAGAAGATTTAAGCTTAC+AGG	0.214140	3.1:+2245428	MS.gene009347:CDS
ACTATTTCCATTTCTCAATA+AGG	0.240811	3.1:-2245556	None:intergenic
CTCGTTGGAATTGCTGAAAA+TGG	0.258071	3.1:+2245527	MS.gene009347:CDS
AGATTTAAGCTTACAGGATA+TGG	0.297921	3.1:+2245434	MS.gene009347:CDS
GCTCCTTATCATATAATAAA+CGG	0.369390	3.1:-2245368	None:intergenic
AAGAAATTCGTGCTAGTTAC+CGG	0.375273	3.1:+2245217	MS.gene009347:CDS
ATCCCGTTTATTATATGATA+AGG	0.385875	3.1:+2245365	MS.gene009347:CDS
GGGTATTCCTTATTGAGAAA+TGG	0.387591	3.1:+2245549	MS.gene009347:CDS
TCGTTGGAATTGCTGAAAAT+GGG	0.404006	3.1:+2245528	MS.gene009347:CDS
TTATGAGGTTCTCAATGTTA+AGG	0.412571	3.1:+2245179	MS.gene009347:CDS
CTCAATGGTGATACTTTACC+AGG	0.416326	3.1:+2245588	MS.gene009347:CDS
AGCTCGTCTGATACTCAGTA+TGG	0.427425	3.1:+2245647	MS.gene009347:CDS
CTCCTTATCATATAATAAAC+GGG	0.433842	3.1:-2245367	None:intergenic
GAGATTTCTCAAAGTTCAAA+AGG	0.446313	3.1:+2245317	MS.gene009347:CDS
TTCTCAAAGTTCAAAAGGCA+TGG	0.453193	3.1:+2245322	MS.gene009347:CDS
CGTACTTGTTTGATCACTGT+TGG	0.461429	3.1:-2245292	None:intergenic
CATTGAGGAAACTCATTATG+AGG	0.462336	3.1:+2245164	MS.gene009347:CDS
AAGATCAAGGCGGGATGCCT+TGG	0.472867	3.1:+2245395	MS.gene009347:CDS
CAAGGAAGAATCACGGATCC+TGG	0.489467	3.1:-2245606	None:intergenic
TTCTGCAATTTCAGCAGCCA+AGG	0.518010	3.1:-2245412	None:intergenic
AATAGTATATCTATACTCAA+TGG	0.536490	3.1:+2245573	MS.gene009347:CDS
TCTTGACAAGCATGACATTG+AGG	0.539456	3.1:+2245149	MS.gene009347:CDS
TCTCAAAGTTCAAAAGGCAT+GGG	0.541609	3.1:+2245323	MS.gene009347:CDS
TTACTTCTCTCTCGACTCGT+TGG	0.542824	3.1:+2245512	MS.gene009347:CDS
ATAAGGAGCTGCAAAGATCA+AGG	0.544696	3.1:+2245382	MS.gene009347:CDS
GGAGCTGCAAAGATCAAGGC+GGG	0.575943	3.1:+2245386	MS.gene009347:CDS
GGATCCGTGATTCTTCCTTG+TGG	0.587718	3.1:+2245609	MS.gene009347:CDS
CAACAGTGATCAAACAAGTA+CGG	0.595965	3.1:+2245293	MS.gene009347:CDS
CGTTGGAATTGCTGAAAATG+GGG	0.627347	3.1:+2245529	MS.gene009347:CDS
AGATCCACAAGGAAGAATCA+CGG	0.662581	3.1:-2245613	None:intergenic
GAGAGAAGTAATCACCACAT+CGG	0.695257	3.1:-2245500	None:intergenic
GTAAACAGAGTACAGCACTC+CGG	0.713129	3.1:-2245236	None:intergenic
TTTAACGAACAAGATCCACA+AGG	0.727941	3.1:-2245624	None:intergenic
AGGAGCTGCAAAGATCAAGG+CGG	0.734461	3.1:+2245385	MS.gene009347:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGTATATCTATACTCAA+TGG	+	chr3.1:2245573-2245592	MS.gene009347:CDS	20.0%
!	ACTATTTCCATTTCTCAATA+AGG	-	chr3.1:2245559-2245578	None:intergenic	25.0%
!	ATCCCGTTTATTATATGATA+AGG	+	chr3.1:2245365-2245384	MS.gene009347:CDS	25.0%
!	CTCCTTATCATATAATAAAC+GGG	-	chr3.1:2245370-2245389	None:intergenic	25.0%
!	GCTCCTTATCATATAATAAA+CGG	-	chr3.1:2245371-2245390	None:intergenic	25.0%
	AGATTTAAGCTTACAGGATA+TGG	+	chr3.1:2245434-2245453	MS.gene009347:CDS	30.0%
	GAGATTTCTCAAAGTTCAAA+AGG	+	chr3.1:2245317-2245336	MS.gene009347:CDS	30.0%
	TTATGAGGTTCTCAATGTTA+AGG	+	chr3.1:2245179-2245198	MS.gene009347:CDS	30.0%
	AAGAAATTCGTGCTAGTTAC+CGG	+	chr3.1:2245217-2245236	MS.gene009347:CDS	35.0%
	CAACAGTGATCAAACAAGTA+CGG	+	chr3.1:2245293-2245312	MS.gene009347:CDS	35.0%
	CATTGAGGAAACTCATTATG+AGG	+	chr3.1:2245164-2245183	MS.gene009347:CDS	35.0%
	GGGTATTCCTTATTGAGAAA+TGG	+	chr3.1:2245549-2245568	MS.gene009347:CDS	35.0%
	TCGTTGGAATTGCTGAAAAT+GGG	+	chr3.1:2245528-2245547	MS.gene009347:CDS	35.0%
	TGCAGAAGATTTAAGCTTAC+AGG	+	chr3.1:2245428-2245447	MS.gene009347:CDS	35.0%
	TTCTCAAAGTTCAAAAGGCA+TGG	+	chr3.1:2245322-2245341	MS.gene009347:CDS	35.0%
	TTTAACGAACAAGATCCACA+AGG	-	chr3.1:2245627-2245646	None:intergenic	35.0%
!	TCTCAAAGTTCAAAAGGCAT+GGG	+	chr3.1:2245323-2245342	MS.gene009347:CDS	35.0%
	AGATCCACAAGGAAGAATCA+CGG	-	chr3.1:2245616-2245635	None:intergenic	40.0%
	ATAAGGAGCTGCAAAGATCA+AGG	+	chr3.1:2245382-2245401	MS.gene009347:CDS	40.0%
	CGTACTTGTTTGATCACTGT+TGG	-	chr3.1:2245295-2245314	None:intergenic	40.0%
	CGTTGGAATTGCTGAAAATG+GGG	+	chr3.1:2245529-2245548	MS.gene009347:CDS	40.0%
	CTCAATGGTGATACTTTACC+AGG	+	chr3.1:2245588-2245607	MS.gene009347:CDS	40.0%
	CTCGTTGGAATTGCTGAAAA+TGG	+	chr3.1:2245527-2245546	MS.gene009347:CDS	40.0%
	GAGAGAAGTAATCACCACAT+CGG	-	chr3.1:2245503-2245522	None:intergenic	40.0%
	TCTTGACAAGCATGACATTG+AGG	+	chr3.1:2245149-2245168	MS.gene009347:CDS	40.0%
!	CTGTTTTATCAATGCCGATG+TGG	+	chr3.1:2245486-2245505	MS.gene009347:CDS	40.0%
	AGCTCGTCTGATACTCAGTA+TGG	+	chr3.1:2245647-2245666	MS.gene009347:CDS	45.0%
	GTAAACAGAGTACAGCACTC+CGG	-	chr3.1:2245239-2245258	None:intergenic	45.0%
	TTCTGCAATTTCAGCAGCCA+AGG	-	chr3.1:2245415-2245434	None:intergenic	45.0%
!	TTACTTCTCTCTCGACTCGT+TGG	+	chr3.1:2245512-2245531	MS.gene009347:CDS	45.0%
	AGGAGCTGCAAAGATCAAGG+CGG	+	chr3.1:2245385-2245404	MS.gene009347:CDS	50.0%
	CAAGGAAGAATCACGGATCC+TGG	-	chr3.1:2245609-2245628	None:intergenic	50.0%
	GGATCCGTGATTCTTCCTTG+TGG	+	chr3.1:2245609-2245628	MS.gene009347:CDS	50.0%
	AAGATCAAGGCGGGATGCCT+TGG	+	chr3.1:2245395-2245414	MS.gene009347:CDS	55.0%
	GGAGCTGCAAAGATCAAGGC+GGG	+	chr3.1:2245386-2245405	MS.gene009347:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	2245144	2245680	2245144	ID=MS.gene009347
chr3.1	mRNA	2245144	2245680	2245144	ID=MS.gene009347.t1;Parent=MS.gene009347
chr3.1	exon	2245144	2245680	2245144	ID=MS.gene009347.t1.exon1;Parent=MS.gene009347.t1
chr3.1	CDS	2245144	2245680	2245144	ID=cds.MS.gene009347.t1;Parent=MS.gene009347.t1

Gene Sequence

>MS.gene009347

ATGATTCTTGACAAGCATGACATTGAGGAAACTCATTATGAGGTTCTCAATGTTAAGGAAGATGCAGATTATGAAGAAATTCGTGCTAGTTACCGGAGTGCTGTACTCTGTTTACATCTTGATAAGCTATTGAAGACATTTGATACATCCAACAGTGATCAAACAAGTACGGAGAGATTTCTCAAAGTTCAAAAGGCATGGGAAATTCTAAGCGATTCAAGATCCCGTTTATTATATGATAAGGAGCTGCAAAGATCAAGGCGGGATGCCTTGGCTGCTGAAATTGCAGAAGATTTAAGCTTACAGGATATGGCTGTTGAAGATGCTGATGAAGCGTTAGAACTGTTTTATCAATGCCGATGTGGTGATTACTTCTCTCTCGACTCGTTGGAATTGCTGAAAATGGGGTATTCCTTATTGAGAAATGGAAATAGTATATCTATACTCAATGGTGATACTTTACCAGGATCCGTGATTCTTCCTTGTGGATCTTGTTCGTTAAAAGCTCGTCTGATACTCAGTATGGATAACAATTGA

Protein sequence

>MS.gene009347.t1

MILDKHDIEETHYEVLNVKEDADYEEIRASYRSAVLCLHLDKLLKTFDTSNSDQTSTERFLKVQKAWEILSDSRSRLLYDKELQRSRRDALAAEIAEDLSLQDMAVEDADEALELFYQCRCGDYFSLDSLELLKMGYSLLRNGNSISILNGDTLPGSVILPCGSCSLKARLILSMDNN