AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83474

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83474.t1 MTR_5g026550 97.674 172 4 0 1 172 1 172 8.69e-125 348
MS.gene83474.t1 MTR_2g048680 30.872 149 94 3 11 156 80 222 4.21e-13 65.1
MS.gene83474.t1 MTR_2g048680 30.872 149 94 3 11 156 160 302 5.40e-13 65.9
MS.gene83474.t1 MTR_5g043640 37.000 100 57 2 26 125 87 180 5.60e-12 62.4
MS.gene83474.t1 MTR_2g096190 38.136 118 64 4 26 141 132 242 2.88e-11 61.2
MS.gene83474.t1 MTR_6g027840 37.000 100 57 2 26 125 394 487 6.74e-11 60.1
MS.gene83474.t1 MTR_3g491870 33.645 107 65 2 27 133 235 335 8.27e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83474.t1 AT5G07840 69.143 175 51 2 1 172 1 175 2.16e-84 246
MS.gene83474.t1 AT5G61230 71.519 158 41 2 1 156 1 156 3.06e-78 230
MS.gene83474.t1 AT2G03430 43.000 100 51 2 26 125 80 173 2.52e-13 65.9
MS.gene83474.t1 AT5G53470 32.258 124 75 4 32 155 221 335 2.86e-11 61.2
MS.gene83474.t1 AT5G40160 30.556 144 90 4 18 157 171 308 3.16e-11 60.8
MS.gene83474.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.06e-11 60.5
MS.gene83474.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.06e-11 60.5
MS.gene83474.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.51e-11 60.5
MS.gene83474.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.51e-11 60.5
MS.gene83474.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.51e-11 60.5

Find 61 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACTTTGTCGCAACGAAAT+TGG 0.234333 5.4:-17415107 None:intergenic
CTTAGTTCTGGCATCAATAT+TGG 0.262291 5.4:-17416944 None:intergenic
TCCTCTCTTTAGCTGCAATA+TGG 0.279082 5.4:-17417096 None:intergenic
GGGGTCACGCCTTTAGATTT+TGG 0.294756 5.4:-17416859 None:intergenic
CGAAGGAGGTTGTTGAGGCC+TGG 0.313233 5.4:+17415134 MS.gene83474:CDS
TTGCAGCTAAAGAGAGGAAT+AGG 0.325717 5.4:+17417101 MS.gene83474:CDS
GAAGGAGGTTGTTGAGGCCT+GGG 0.337003 5.4:+17415135 MS.gene83474:CDS
ATTACTGCCCAGGACTTGAA+TGG 0.394427 5.4:+17417203 MS.gene83474:CDS
TAGGGATGCTGTGAAATTCT+TGG 0.430272 5.4:+17417120 MS.gene83474:CDS
TGTGAGAGAAGACATGCTTC+AGG 0.432571 5.4:+17415079 None:intergenic
GCCACAAGCACCCTTAGTTC+TGG 0.432585 5.4:-17416956 None:intergenic
TCATAGGCCCATTCAAGTCC+TGG 0.450896 5.4:-17417211 None:intergenic
AAGTCTCTTCATTTCCTCAT+AGG 0.467730 5.4:-17417227 None:intergenic
CAATATTGATGCCAGAACTA+AGG 0.481725 5.4:+17416945 MS.gene83474:CDS
TTCGACAGCAAGAATTATCT+CGG 0.489219 5.4:+17417248 MS.gene83474:CDS
CTCCACCTTGCTGCCGAAGG+TGG 0.490968 5.4:+17416883 MS.gene83474:CDS
CACTCCGAGATGACCACCTT+CGG 0.497622 5.4:-17416896 None:intergenic
GCTGCCGAAGGTGGTCATCT+CGG 0.507384 5.4:+17416892 MS.gene83474:CDS
TTACTGCCCAGGACTTGAAT+GGG 0.512016 5.4:+17417204 MS.gene83474:CDS
GTGAAATTCTTGGTAGAGAA+TGG 0.513179 5.4:+17417130 MS.gene83474:CDS
CTTCGGCAGCAAGGTGGAGA+GGG 0.515210 5.4:-17416879 None:intergenic
TGCAGCTAAAGAGAGGAATA+GGG 0.519744 5.4:+17417102 MS.gene83474:CDS
AGGACTTGAATGGGCCTATG+AGG 0.531270 5.4:+17417213 MS.gene83474:CDS
CCTCTCCACCTTGCTGCCGA+AGG 0.532516 5.4:+17416880 MS.gene83474:CDS
AATCTGCTATCATTGATATC+CGG 0.533897 5.4:-17417166 None:intergenic
AAAGCTTCTCAACGAAGGAA+TGG 0.535311 5.4:+17416813 MS.gene83474:CDS
TTGATACTGATGATAGAGGT+TGG 0.535680 5.4:+17415159 MS.gene83474:CDS
CCTCCTCTTCATTACTGCCC+AGG 0.539604 5.4:+17417193 MS.gene83474:CDS
AGGTGGTCATCTCGGAGTGA+TGG 0.540638 5.4:+17416900 MS.gene83474:CDS
CTTCATGTTTATGCAAGGAA+AGG 0.547906 5.4:+17415188 MS.gene83474:CDS
AATATTGATGCCAGAACTAA+GGG 0.549077 5.4:+17416946 MS.gene83474:CDS
AAGGAGGTTGTTGAGGCCTG+GGG 0.557016 5.4:+17415136 MS.gene83474:CDS
GCATGGGGCCCAAAATCTAA+AGG 0.559905 5.4:+17416850 MS.gene83474:CDS
CATAGGCCCATTCAAGTCCT+GGG 0.560704 5.4:-17417210 None:intergenic
CCTTCGGCAGCAAGGTGGAG+AGG 0.564920 5.4:-17416880 None:intergenic
AAGGAAAGGTGATATTAAGC+TGG 0.572436 5.4:+17415202 MS.gene83474:CDS
GAATGGAGCATTCTTGCCAC+CGG 0.577208 5.4:+17417147 MS.gene83474:CDS
TGGATGTTAATGTGACTGCA+TGG 0.583622 5.4:+17416833 MS.gene83474:CDS
CCTCACTTCATGTTTATGCA+AGG 0.587941 5.4:+17415183 MS.gene83474:CDS
CTTCGCGAAGGAGGTTGTTG+AGG 0.590897 5.4:+17415129 MS.gene83474:CDS
TCGACAGCAAGAATTATCTC+GGG 0.593381 5.4:+17417249 MS.gene83474:CDS
TCCATATTGCAGCTAAAGAG+AGG 0.596526 5.4:+17417095 MS.gene83474:CDS
CTATCATCAGTATCAACCCC+AGG 0.598054 5.4:-17415152 None:intergenic
GATGACCACCTTCGGCAGCA+AGG 0.610444 5.4:-17416888 None:intergenic
TTCGGCAGCAAGGTGGAGAG+GGG 0.614770 5.4:-17416878 None:intergenic
GCCAGAACTAAGGGTGCTTG+TGG 0.615169 5.4:+17416955 MS.gene83474:CDS
CTCAACAACCTCCTTCGCGA+AGG 0.617083 5.4:-17415128 None:intergenic
GACAAAGTCCTTCGCGAAGG+AGG 0.622537 5.4:+17415120 MS.gene83474:CDS
AGTCCTGGGCAGTAATGAAG+AGG 0.632969 5.4:-17417196 None:intergenic
GAGAAGACATGCTTCAGGAG+AGG 0.635852 5.4:+17415084 None:intergenic
CCTGGGCAGTAATGAAGAGG+AGG 0.640191 5.4:-17417193 None:intergenic
TCTTTAGCTGCAATATGGAG+CGG 0.641697 5.4:-17417091 None:intergenic
GGATGTTAATGTGACTGCAT+GGG 0.642233 5.4:+17416834 MS.gene83474:CDS
TGCGACAAAGTCCTTCGCGA+AGG 0.643930 5.4:+17415117 MS.gene83474:CDS
CTGCTATCATTGATATCCGG+TGG 0.644744 5.4:-17417163 None:intergenic
CCTTGCATAAACATGAAGTG+AGG 0.647744 5.4:-17415183 None:intergenic
GGGGTTGATACTGATGATAG+AGG 0.648484 5.4:+17415155 MS.gene83474:CDS
GATGTTAATGTGACTGCATG+GGG 0.662007 5.4:+17416835 MS.gene83474:CDS
ACAGCAAGAATTATCTCGGG+AGG 0.678526 5.4:+17417252 MS.gene83474:CDS
GACCACCTTCGGCAGCAAGG+TGG 0.692091 5.4:-17416885 None:intergenic
ATGGATGAACTGCTTGAGCG+TGG 0.700290 5.4:+17416919 MS.gene83474:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGAATGTTTTATTATGA+GGG + chr5.4:17415312-17415331 MS.gene83474:intron 15.0%
!!! TATATGAATGTTTTATTATG+AGG + chr5.4:17415311-17415330 MS.gene83474:intron 15.0%
!! AACCAAATAAATCTAAGTAA+AGG - chr5.4:17415399-17415418 None:intergenic 20.0%
!! ACCAAATAAATCTAAGTAAA+GGG - chr5.4:17415398-17415417 None:intergenic 20.0%
!! ATTTGTCTAATATTCACATA+AGG + chr5.4:17415529-17415548 MS.gene83474:intron 20.0%
!! TATAATATCATTAGGAGATA+AGG + chr5.4:17415567-17415586 MS.gene83474:intron 20.0%
!! TTATACTTAAAAATCCTGTT+AGG - chr5.4:17416345-17416364 None:intergenic 20.0%
!!! AAATTGACTATGTTTTACTT+CGG + chr5.4:17415257-17415276 MS.gene83474:intron 20.0%
!!! CCATATTTTATTTTGTAGAT+GGG + chr5.4:17415468-17415487 MS.gene83474:intron 20.0%
!!! TGATAATTTTCTACTCTTAA+AGG - chr5.4:17416733-17416752 None:intergenic 20.0%
! AAGATAAATAATGTGCAAGA+AGG + chr5.4:17415358-17415377 MS.gene83474:intron 25.0%
! AAGTTATGACTAATGAGTTA+AGG + chr5.4:17416091-17416110 MS.gene83474:intron 25.0%
! AATAAAATATGGCTTTCAGT+AGG - chr5.4:17415460-17415479 None:intergenic 25.0%
! ACTAATATGGAAGTCTATAT+GGG - chr5.4:17416999-17417018 None:intergenic 25.0%
! AGTTATGACTAATGAGTTAA+GGG + chr5.4:17416092-17416111 MS.gene83474:intron 25.0%
! ATGTATTCTCAAAAACATAG+AGG + chr5.4:17415726-17415745 MS.gene83474:intron 25.0%
! ATTAACAAACAGTCGTTATT+GGG + chr5.4:17416477-17416496 MS.gene83474:intron 25.0%
! CCCATCTACAAAATAAAATA+TGG - chr5.4:17415471-17415490 None:intergenic 25.0%
! GACAGATAGATATACTAATA+TGG - chr5.4:17417012-17417031 None:intergenic 25.0%
! TACTAATATGGAAGTCTATA+TGG - chr5.4:17417000-17417019 None:intergenic 25.0%
!! AATTTTACAGTGTGCTATAT+TGG + chr5.4:17415854-17415873 MS.gene83474:intron 25.0%
!!! ATTTTATTTTGTAGATGGGA+GGG + chr5.4:17415472-17415491 MS.gene83474:intron 25.0%
!!! GCCATATTTTATTTTGTAGA+TGG + chr5.4:17415467-17415486 MS.gene83474:intron 25.0%
!!! TATTTTATTTTGTAGATGGG+AGG + chr5.4:17415471-17415490 MS.gene83474:intron 25.0%
!!! TTAGATTTATTTGGTTTGAG+TGG + chr5.4:17415403-17415422 MS.gene83474:intron 25.0%
!!! TTATAATGTTGTTTCTGAAC+AGG + chr5.4:17416786-17416805 MS.gene83474:intron 25.0%
AATATTGATGCCAGAACTAA+GGG + chr5.4:17416946-17416965 MS.gene83474:CDS 30.0%
AATCTGCTATCATTGATATC+CGG - chr5.4:17417169-17417188 None:intergenic 30.0%
AGGTTGAATTTGAATAGTAC+AGG + chr5.4:17415790-17415809 MS.gene83474:intron 30.0%
ATTATCACACTTGAAGTGTA+AGG + chr5.4:17416746-17416765 MS.gene83474:intron 30.0%
CATTAACAAACAGTCGTTAT+TGG + chr5.4:17416476-17416495 MS.gene83474:intron 30.0%
GCCCTTTACTTAGATTTATT+TGG + chr5.4:17415394-17415413 MS.gene83474:intron 30.0%
GGTTGAATTTGAATAGTACA+GGG + chr5.4:17415791-17415810 MS.gene83474:intron 30.0%
TAACTAATTGCAACCATCTA+AGG - chr5.4:17416294-17416313 None:intergenic 30.0%
TAATCCAGCTATATGAATTG+AGG + chr5.4:17415696-17415715 MS.gene83474:intron 30.0%
TGGTGAATTTGTAGAAGTAA+CGG + chr5.4:17416126-17416145 MS.gene83474:intron 30.0%
TGTCTAATATTCACATAAGG+TGG + chr5.4:17415532-17415551 MS.gene83474:intron 30.0%
TTAGATGGTTGCAATTAGTT+AGG + chr5.4:17416293-17416312 MS.gene83474:intron 30.0%
TTATCACACTTGAAGTGTAA+GGG + chr5.4:17416747-17416766 MS.gene83474:intron 30.0%
TTCTATACAGCTCTTGTAAA+AGG + chr5.4:17416368-17416387 MS.gene83474:intron 30.0%
! ATTTTGTTCTCCTTAAACCA+GGG + chr5.4:17417065-17417084 MS.gene83474:intron 30.0%
! GCTATCAATCTAGAGTTATT+TGG - chr5.4:17416033-17416052 None:intergenic 30.0%
! GGTCAATTTTCTTGAGATTT+AGG + chr5.4:17415609-17415628 MS.gene83474:intron 30.0%
! TGTTTTACTTCGGAATTTTG+TGG + chr5.4:17415267-17415286 MS.gene83474:intron 30.0%
! TTTTGTAAACACAGCAATGA+AGG - chr5.4:17416608-17416627 None:intergenic 30.0%
!!! GGAGGGTTTTTTTTAACATT+TGG + chr5.4:17415489-17415508 MS.gene83474:intron 30.0%
!!! GGTTAGTTTCTGCATTTTAT+TGG + chr5.4:17415588-17415607 MS.gene83474:intron 30.0%
AACTTGCAATTGAAAGTCGT+TGG + chr5.4:17415884-17415903 MS.gene83474:intron 35.0%
AAGGAAAGGTGATATTAAGC+TGG + chr5.4:17415202-17415221 MS.gene83474:CDS 35.0%
AAGTCTCTTCATTTCCTCAT+AGG - chr5.4:17417230-17417249 None:intergenic 35.0%
CAATATTGATGCCAGAACTA+AGG + chr5.4:17416945-17416964 MS.gene83474:CDS 35.0%
CAATCAGTTGCACATTATGT+TGG + chr5.4:17415929-17415948 MS.gene83474:intron 35.0%
CTTCATGTTTATGCAAGGAA+AGG + chr5.4:17415188-17415207 MS.gene83474:CDS 35.0%
GGTACAGTTAAACATTGGAA+GGG + chr5.4:17415905-17415924 MS.gene83474:intron 35.0%
GGTGAATTTGTAGAAGTAAC+GGG + chr5.4:17416127-17416146 MS.gene83474:intron 35.0%
GTAAAAAAGCTTCTCAACGA+AGG + chr5.4:17416808-17416827 MS.gene83474:CDS 35.0%
GTAGATGAATCTACTGAGAA+GGG + chr5.4:17415755-17415774 MS.gene83474:intron 35.0%
GTGAAATTCTTGGTAGAGAA+TGG + chr5.4:17417130-17417149 MS.gene83474:CDS 35.0%
TACATTTGTAGAGTGTGAAG+TGG + chr5.4:17416200-17416219 MS.gene83474:intron 35.0%
TCGTTGGTACAGTTAAACAT+TGG + chr5.4:17415900-17415919 MS.gene83474:intron 35.0%
TGGTACAGTTAAACATTGGA+AGG + chr5.4:17415904-17415923 MS.gene83474:intron 35.0%
TGTACCTCAATTCATATAGC+TGG - chr5.4:17415703-17415722 None:intergenic 35.0%
TTCGACAGCAAGAATTATCT+CGG + chr5.4:17417248-17417267 MS.gene83474:CDS 35.0%
TTGATACTGATGATAGAGGT+TGG + chr5.4:17415159-17415178 MS.gene83474:CDS 35.0%
! ATCATCGACTAAAGTAAGCA+AGG - chr5.4:17416676-17416695 None:intergenic 35.0%
! ATTGATAGCATAGCATTGAG+AGG + chr5.4:17416044-17416063 MS.gene83474:intron 35.0%
! GATTTTGTTCTCCTTAAACC+AGG + chr5.4:17417064-17417083 MS.gene83474:intron 35.0%
! GCTTCAGTTATGAGATTTTG+GGG + chr5.4:17415425-17415444 MS.gene83474:intron 35.0%
! GGCTTCAGTTATGAGATTTT+GGG + chr5.4:17415424-17415443 MS.gene83474:intron 35.0%
! TGGCTTCAGTTATGAGATTT+TGG + chr5.4:17415423-17415442 MS.gene83474:intron 35.0%
! TTGATAGCATAGCATTGAGA+GGG + chr5.4:17416045-17416064 MS.gene83474:intron 35.0%
!! CTTAGTTCTGGCATCAATAT+TGG - chr5.4:17416947-17416966 None:intergenic 35.0%
AAAGCTTCTCAACGAAGGAA+TGG + chr5.4:17416813-17416832 MS.gene83474:CDS 40.0%
AATAGTACAGGGCTAAAGAG+GGG + chr5.4:17415802-17415821 MS.gene83474:intron 40.0%
AGGGGTTGTTTGTAGATTCA+TGG + chr5.4:17416064-17416083 MS.gene83474:intron 40.0%
ATGAATCTACTGAGAAGGGA+AGG + chr5.4:17415759-17415778 MS.gene83474:intron 40.0%
CAATTTACCGAAGTGATTGC+TGG + chr5.4:17416415-17416434 MS.gene83474:intron 40.0%
CATAAGACCAGCAATCACTT+CGG - chr5.4:17416425-17416444 None:intergenic 40.0%
CCTCACTTCATGTTTATGCA+AGG + chr5.4:17415183-17415202 MS.gene83474:CDS 40.0%
CCTTGCATAAACATGAAGTG+AGG - chr5.4:17415186-17415205 None:intergenic 40.0%
CGTAGATGAATCTACTGAGA+AGG + chr5.4:17415754-17415773 MS.gene83474:intron 40.0%
CTAGTCTACACCTTCTATGT+TGG - chr5.4:17416227-17416246 None:intergenic 40.0%
GAATAGTACAGGGCTAAAGA+GGG + chr5.4:17415801-17415820 MS.gene83474:intron 40.0%
GAGAAGGGAAGGTTATTCTT+AGG + chr5.4:17415770-17415789 MS.gene83474:intron 40.0%
GATGTTAATGTGACTGCATG+GGG + chr5.4:17416835-17416854 MS.gene83474:CDS 40.0%
GGATGTTAATGTGACTGCAT+GGG + chr5.4:17416834-17416853 MS.gene83474:CDS 40.0%
GTCGATGATTCATGAACTGT+TGG + chr5.4:17416687-17416706 MS.gene83474:intron 40.0%
GTTACGCAGTATTGTTCACA+CGG + chr5.4:17416318-17416337 MS.gene83474:intron 40.0%
TAATGAGTTAAGGGTGACCA+GGG + chr5.4:17416101-17416120 MS.gene83474:intron 40.0%
TAGGGATGCTGTGAAATTCT+TGG + chr5.4:17417120-17417139 MS.gene83474:CDS 40.0%
TCCATATTGCAGCTAAAGAG+AGG + chr5.4:17417095-17417114 MS.gene83474:CDS 40.0%
TCCTCTCTTTAGCTGCAATA+TGG - chr5.4:17417099-17417118 None:intergenic 40.0%
TCGACAGCAAGAATTATCTC+GGG + chr5.4:17417249-17417268 MS.gene83474:CDS 40.0%
TCTTTAGCTGCAATATGGAG+CGG - chr5.4:17417094-17417113 None:intergenic 40.0%
TGAATAGTACAGGGCTAAAG+AGG + chr5.4:17415800-17415819 MS.gene83474:intron 40.0%
TGCAGCTAAAGAGAGGAATA+GGG + chr5.4:17417102-17417121 MS.gene83474:CDS 40.0%
TGGATGTTAATGTGACTGCA+TGG + chr5.4:17416833-17416852 MS.gene83474:CDS 40.0%
TTGCAGCTAAAGAGAGGAAT+AGG + chr5.4:17417101-17417120 MS.gene83474:CDS 40.0%
! AAAGGTGCAAGGAATGGTTA+TGG + chr5.4:17416386-17416405 MS.gene83474:intron 40.0%
! AAGTTGGTTGCAAGCATCAA+CGG - chr5.4:17416525-17416544 None:intergenic 40.0%
! ACACAGCAATGAAGGTGTAA+AGG - chr5.4:17416600-17416619 None:intergenic 40.0%
! CACAGCAATGAAGGTGTAAA+GGG - chr5.4:17416599-17416618 None:intergenic 40.0%
! CATAGAAGGTGTAGACTAGA+TGG + chr5.4:17416228-17416247 MS.gene83474:intron 40.0%
! CTTGTAAAAGGTGCAAGGAA+TGG + chr5.4:17416380-17416399 MS.gene83474:intron 40.0%
! GATGACAAGTTTGTTTGACG+AGG + chr5.4:17416250-17416269 MS.gene83474:intron 40.0%
! TGACTTGCAAGATCCTTAGA+TGG + chr5.4:17416278-17416297 MS.gene83474:intron 40.0%
! TGATAGCATAGCATTGAGAG+GGG + chr5.4:17416046-17416065 MS.gene83474:intron 40.0%
!!! TTTTAGCTCCCAACATGCAT+CGG - chr5.4:17416560-17416579 None:intergenic 40.0%
AACATGCATCGGGGAATTTG+GGG - chr5.4:17416549-17416568 None:intergenic 45.0%
AATGAGTTAAGGGTGACCAG+GGG + chr5.4:17416102-17416121 MS.gene83474:intron 45.0%
ACAACCAGAACTGTGTCAAC+TGG - chr5.4:17416451-17416470 None:intergenic 45.0%
ACAGCAAGAATTATCTCGGG+AGG + chr5.4:17417252-17417271 MS.gene83474:CDS 45.0%
ATTACTGCCCAGGACTTGAA+TGG + chr5.4:17417203-17417222 MS.gene83474:CDS 45.0%
ATTGTTCACACGGACCTAAC+AGG + chr5.4:17416328-17416347 MS.gene83474:intron 45.0%
CAAATTCCCCGATGCATGTT+GGG + chr5.4:17416549-17416568 MS.gene83474:intron 45.0%
CAACATGCATCGGGGAATTT+GGG - chr5.4:17416550-17416569 None:intergenic 45.0%
CTAATGAGTTAAGGGTGACC+AGG + chr5.4:17416100-17416119 MS.gene83474:intron 45.0%
CTATCATCAGTATCAACCCC+AGG - chr5.4:17415155-17415174 None:intergenic 45.0%
CTGCTATCATTGATATCCGG+TGG - chr5.4:17417166-17417185 None:intergenic 45.0%
GACAGTTATGACCAGAAGAG+GGG + chr5.4:17415951-17415970 MS.gene83474:intron 45.0%
GGACAGTTATGACCAGAAGA+GGG + chr5.4:17415950-17415969 MS.gene83474:intron 45.0%
GGACTTTGTCGCAACGAAAT+TGG - chr5.4:17415110-17415129 None:intergenic 45.0%
GTGAAGTGGTCCAACATAGA+AGG + chr5.4:17416214-17416233 MS.gene83474:intron 45.0%
TAAAGGGTGACTAGTGCACA+AGG - chr5.4:17416583-17416602 None:intergenic 45.0%
TCAGAGTCACACTTCTAGAC+TGG - chr5.4:17416159-17416178 None:intergenic 45.0%
TCTACAAATTCACCAACCCC+TGG - chr5.4:17416121-17416140 None:intergenic 45.0%
TGGACAGTTATGACCAGAAG+AGG + chr5.4:17415949-17415968 MS.gene83474:intron 45.0%
TGGGTATGAGATACGACTGT+AGG + chr5.4:17416496-17416515 MS.gene83474:intron 45.0%
TTACTGCCCAGGACTTGAAT+GGG + chr5.4:17417204-17417223 MS.gene83474:CDS 45.0%
! CAGCTCTTGTAAAAGGTGCA+AGG + chr5.4:17416375-17416394 MS.gene83474:intron 45.0%
! GGGTCACGCCTTTAGATTTT+GGG - chr5.4:17416861-17416880 None:intergenic 45.0%
! TTTAGCTCCCAACATGCATC+GGG - chr5.4:17416559-17416578 None:intergenic 45.0%
!! GGGGTTGATACTGATGATAG+AGG + chr5.4:17415155-17415174 MS.gene83474:CDS 45.0%
!! AATAAAAAAAAATCAAAATT+TGG - chr5.4:17417041-17417060 None:intergenic 5.0%
!!! TTTTTTAATATAATATCATT+AGG + chr5.4:17415559-17415578 MS.gene83474:intron 5.0%
AGGACTTGAATGGGCCTATG+AGG + chr5.4:17417213-17417232 MS.gene83474:CDS 50.0%
AGTCCTGGGCAGTAATGAAG+AGG - chr5.4:17417199-17417218 None:intergenic 50.0%
ATGGATGAACTGCTTGAGCG+TGG + chr5.4:17416919-17416938 MS.gene83474:CDS 50.0%
ATGTGCAGAGCAGCTGTAGA+TGG + chr5.4:17415993-17416012 MS.gene83474:intron 50.0%
CATAGGCCCATTCAAGTCCT+GGG - chr5.4:17417213-17417232 None:intergenic 50.0%
CCAAATTCCCCGATGCATGT+TGG + chr5.4:17416548-17416567 MS.gene83474:intron 50.0%
CCAACATGCATCGGGGAATT+TGG - chr5.4:17416551-17416570 None:intergenic 50.0%
GAGACCAGTTGACACAGTTC+TGG + chr5.4:17416444-17416463 MS.gene83474:intron 50.0%
GCATGGGGCCCAAAATCTAA+AGG + chr5.4:17416850-17416869 MS.gene83474:CDS 50.0%
TCATAGGCCCATTCAAGTCC+TGG - chr5.4:17417214-17417233 None:intergenic 50.0%
TGTGCAGAGCAGCTGTAGAT+GGG + chr5.4:17415994-17416013 MS.gene83474:intron 50.0%
TTAGCTCCCAACATGCATCG+GGG - chr5.4:17416558-17416577 None:intergenic 50.0%
TTATGACCAGAAGAGGGGCT+TGG + chr5.4:17415956-17415975 MS.gene83474:intron 50.0%
! GAATGGAGCATTCTTGCCAC+CGG + chr5.4:17417147-17417166 MS.gene83474:CDS 50.0%
! GGGGTCACGCCTTTAGATTT+TGG - chr5.4:17416862-17416881 None:intergenic 50.0%
AAGGAGGTTGTTGAGGCCTG+GGG + chr5.4:17415136-17415155 MS.gene83474:CDS 55.0%
AGGTGGTCATCTCGGAGTGA+TGG + chr5.4:17416900-17416919 MS.gene83474:CDS 55.0%
AGTTAAGGGTGACCAGGGGT+TGG + chr5.4:17416106-17416125 MS.gene83474:intron 55.0%
ATACTGCCAAGCCCCTCTTC+TGG - chr5.4:17415965-17415984 None:intergenic 55.0%
CACTCCGAGATGACCACCTT+CGG - chr5.4:17416899-17416918 None:intergenic 55.0%
CCTCCTCTTCATTACTGCCC+AGG + chr5.4:17417193-17417212 MS.gene83474:CDS 55.0%
CCTGGGCAGTAATGAAGAGG+AGG - chr5.4:17417196-17417215 None:intergenic 55.0%
CTCAACAACCTCCTTCGCGA+AGG - chr5.4:17415131-17415150 None:intergenic 55.0%
CTTCGCGAAGGAGGTTGTTG+AGG + chr5.4:17415129-17415148 MS.gene83474:CDS 55.0%
GAAGGAGGTTGTTGAGGCCT+GGG + chr5.4:17415135-17415154 MS.gene83474:CDS 55.0%
GACAAAGTCCTTCGCGAAGG+AGG + chr5.4:17415120-17415139 MS.gene83474:CDS 55.0%
GCCACAAGCACCCTTAGTTC+TGG - chr5.4:17416959-17416978 None:intergenic 55.0%
GTGCAGAGCAGCTGTAGATG+GGG + chr5.4:17415995-17416014 MS.gene83474:intron 55.0%
TGCGACAAAGTCCTTCGCGA+AGG + chr5.4:17415117-17415136 MS.gene83474:CDS 55.0%
! TCGGGGAATTTGGGGCAAGT+TGG - chr5.4:17416541-17416560 None:intergenic 55.0%
!! GCCAGAACTAAGGGTGCTTG+TGG + chr5.4:17416955-17416974 MS.gene83474:CDS 55.0%
CGAAGGAGGTTGTTGAGGCC+TGG + chr5.4:17415134-17415153 MS.gene83474:CDS 60.0%
CTTCGGCAGCAAGGTGGAGA+GGG - chr5.4:17416882-17416901 None:intergenic 60.0%
GATGACCACCTTCGGCAGCA+AGG - chr5.4:17416891-17416910 None:intergenic 60.0%
GCAATATGGAGCGGTGTCCC+TGG - chr5.4:17417085-17417104 None:intergenic 60.0%
GCTGCCGAAGGTGGTCATCT+CGG + chr5.4:17416892-17416911 MS.gene83474:CDS 60.0%
GGAGCGGTGTCCCTGGTTTA+AGG - chr5.4:17417078-17417097 None:intergenic 60.0%
GTACAGGGCTAAAGAGGGGC+AGG + chr5.4:17415806-17415825 MS.gene83474:intron 60.0%
TTCGGCAGCAAGGTGGAGAG+GGG - chr5.4:17416881-17416900 None:intergenic 60.0%
CCTCTCCACCTTGCTGCCGA+AGG + chr5.4:17416880-17416899 MS.gene83474:CDS 65.0%
CCTTCGGCAGCAAGGTGGAG+AGG - chr5.4:17416883-17416902 None:intergenic 65.0%
CTCCACCTTGCTGCCGAAGG+TGG + chr5.4:17416883-17416902 MS.gene83474:CDS 65.0%
GACCACCTTCGGCAGCAAGG+TGG - chr5.4:17416888-17416907 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 17415092 17417300 17415092 ID=MS.gene83474
chr5.4 mRNA 17415092 17417300 17415092 ID=MS.gene83474.t1;Parent=MS.gene83474
chr5.4 exon 17415092 17415223 17415092 ID=MS.gene83474.t1.exon1;Parent=MS.gene83474.t1
chr5.4 CDS 17415092 17415223 17415092 ID=cds.MS.gene83474.t1;Parent=MS.gene83474.t1
chr5.4 exon 17416808 17416979 17416808 ID=MS.gene83474.t1.exon2;Parent=MS.gene83474.t1
chr5.4 CDS 17416808 17416979 17416808 ID=cds.MS.gene83474.t1;Parent=MS.gene83474.t1
chr5.4 exon 17417086 17417300 17417086 ID=MS.gene83474.t1.exon3;Parent=MS.gene83474.t1
chr5.4 CDS 17417086 17417300 17417086 ID=cds.MS.gene83474.t1;Parent=MS.gene83474.t1
Gene Sequence

>MS.gene83474

ATGCTTCAGGAGAGGCCAATTTCGTTGCGACAAAGTCCTTCGCGAAGGAGGTTGTTGAGGCCTGGGGTTGATACTGATGATAGAGGTTGGACCTCACTTCATGTTTATGCAAGGAAAGGTGATATTAAGCTGGTAAGTTATTTTCTTTTGTTCATTTTTTTATGCAAATTGACTATGTTTTACTTCGGAATTTTGTGGATAAAATTCGAGAAATGTATGTATATGAATGTTTTATTATGAGGGTAAGTTTCATTTTGACGTGCACAAAGATAAATAATGTGCAAGAAGGAAAACTCAAGTGTGCCCTTTACTTAGATTTATTTGGTTTGAGTGGCTTCAGTTATGAGATTTTGGGGCTTTTGTTTCCTACTGAAAGCCATATTTTATTTTGTAGATGGGAGGGTTTTTTTTAACATTTGGTTACATATCTATCATATATTTGTCTAATATTCACATAAGGTGGTTTTTTTTTTAATATAATATCATTAGGAGATAAGGTTAGTTTCTGCATTTTATTGGTCAATTTTCTTGAGATTTAGGTGTGTAGCATATCTCTCAACTTTTTTATCACATAATCGAGCTATATGAAACGAGTTTTGTCACATAATCCAGCTATATGAATTGAGGTACACATATGTATTCTCAAAAACATAGAGGCTCAACGTAGATGAATCTACTGAGAAGGGAAGGTTATTCTTAGGTTGAATTTGAATAGTACAGGGCTAAAGAGGGGCAGGACAGTATGCAAACAATGTATAGAGCAATTTTACAGTGTGCTATATTGGAGCAAATAACTTGCAATTGAAAGTCGTTGGTACAGTTAAACATTGGAAGGGACAATCAGTTGCACATTATGTTGGACAGTTATGACCAGAAGAGGGGCTTGGCAGTATGTCAAACAATGTGCAGAGCAGCTGTAGATGGGGCTATGTGTATTGCCAAATAACTCTAGATTGATAGCATAGCATTGAGAGGGGTTGTTTGTAGATTCATGGTCAGAAGTTATGACTAATGAGTTAAGGGTGACCAGGGGTTGGTGAATTTGTAGAAGTAACGGGTATTCTCCAGTCTAGAAGTGTGACTCTGATTCTGTCAGACATGTAGTATATACATTTGTAGAGTGTGAAGTGGTCCAACATAGAAGGTGTAGACTAGATGGATGACAAGTTTGTTTGACGAGGTTGCGTGACTTGCAAGATCCTTAGATGGTTGCAATTAGTTAGGAAGTTACGCAGTATTGTTCACACGGACCTAACAGGATTTTTAAGTATAAATGTTCTATACAGCTCTTGTAAAAGGTGCAAGGAATGGTTATGGTTACTGCAATTTACCGAAGTGATTGCTGGTCTTATGAGACCAGTTGACACAGTTCTGGTTGTGTAAACATTAACAAACAGTCGTTATTGGGTATGAGATACGACTGTAGGCAACCGTTGATGCTTGCAACCAACTTGCCCCAAATTCCCCGATGCATGTTGGGAGCTAAAACCTTGTGCACTAGTCACCCTTTACACCTTCATTGCTGTGTTTACAAAATATTCAAACTTTTTGCTACTAATTTCTGTTGTGTTTAATCTTTTTCCTTGCTTACTTTAGTCGATGATTCATGAACTGTTGGATTATATATGATTTATTATTCCTTTAAGAGTAGAAAATTATCACACTTGAAGTGTAAGGGAAGCATCTGCTTATATTTATAATGTTGTTTCTGAACAGGTAAAAAAGCTTCTCAACGAAGGAATGGATGTTAATGTGACTGCATGGGGCCCAAAATCTAAAGGCGTGACCCCTCTCCACCTTGCTGCCGAAGGTGGTCATCTCGGAGTGATGGATGAACTGCTTGAGCGTGGTGCCAATATTGATGCCAGAACTAAGGGTGCTTGTGGCTGTAAGTTTTTATGCTTCCCATATAGACTTCCATATTAGTATATCTATCTGTCTAAATTCCAAATTTTGATTTTTTTTTATTCATGATTTTGTTCTCCTTAAACCAGGGACACCGCTCCATATTGCAGCTAAAGAGAGGAATAGGGATGCTGTGAAATTCTTGGTAGAGAATGGAGCATTCTTGCCACCGGATATCAATGATAGCAGATTTAATCCTCCTCTTCATTACTGCCCAGGACTTGAATGGGCCTATGAGGAAATGAAGAGACTTCGACAGCAAGAATTATCTCGGGAGGACACATCTTGCAGCTCTGAAAATTAA
Protein sequence

>MS.gene83474.t1

MLQERPISLRQSPSRRRLLRPGVDTDDRGWTSLHVYARKGDIKLVKKLLNEGMDVNVTAWGPKSKGVTPLHLAAEGGHLGVMDELLERGANIDARTKGACGWTPLHIAAKERNRDAVKFLVENGAFLPPDINDSRFNPPLHYCPGLEWAYEEMKRLRQQELSREDTSCSSEN