AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028111

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028111.t1 MTR_5g026550 98.256 172 3 0 1 172 1 172 3.52e-126 352
MS.gene028111.t1 MTR_2g048680 30.872 149 94 3 11 156 80 222 4.16e-13 65.1
MS.gene028111.t1 MTR_2g048680 30.872 149 94 3 11 156 160 302 5.04e-13 65.9
MS.gene028111.t1 MTR_5g043640 37.000 100 57 2 26 125 87 180 5.66e-12 62.4
MS.gene028111.t1 MTR_2g096190 38.136 118 64 4 26 141 132 242 2.88e-11 61.2
MS.gene028111.t1 MTR_6g027840 37.000 100 57 2 26 125 394 487 5.25e-11 60.5
MS.gene028111.t1 MTR_3g491870 33.645 107 65 2 27 133 235 335 7.66e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028111.t1 AT5G07840 69.143 175 51 2 1 172 1 175 1.66e-84 246
MS.gene028111.t1 AT5G61230 71.519 158 41 2 1 156 1 156 3.03e-78 231
MS.gene028111.t1 AT2G03430 43.000 100 51 2 26 125 80 173 2.65e-13 65.9
MS.gene028111.t1 AT5G40160 30.556 144 90 4 18 157 171 308 2.55e-11 61.2
MS.gene028111.t1 AT5G53470 32.258 124 75 4 32 155 221 335 2.62e-11 61.2
MS.gene028111.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.06e-11 60.5
MS.gene028111.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.06e-11 60.5
MS.gene028111.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.21e-11 60.5
MS.gene028111.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.21e-11 60.5
MS.gene028111.t1 AT5G02620 35.043 117 69 4 26 141 122 232 5.21e-11 60.5
MS.gene028111.t1 AT2G43850 42.708 96 49 2 33 128 82 171 9.74e-11 59.7

Find 62 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACTTTGTCGCAACGAAAT+TGG 0.234333 5.3:-16465671 None:intergenic
CTTAGTTCTGGCATCAATAT+TGG 0.262291 5.3:-16467558 None:intergenic
TCCTCTCTTTAGCTGCAATA+TGG 0.279082 5.3:-16467709 None:intergenic
GGGGTCACGCCTTTAGATTT+AGG 0.293985 5.3:-16467473 None:intergenic
GGGTCACGCCTTTAGATTTA+GGG 0.308028 5.3:-16467472 None:intergenic
CGAAGGAGGTTGTTGAGGCC+TGG 0.313233 5.3:+16465698 MS.gene028111:CDS
TTGCAGCTAAAGAGAGGAAT+AGG 0.325717 5.3:+16467714 MS.gene028111:CDS
GAAGGAGGTTGTTGAGGCCT+GGG 0.337003 5.3:+16465699 MS.gene028111:CDS
ATTACTGCCCAGGACTTGAA+TGG 0.394427 5.3:+16467816 MS.gene028111:CDS
TAGGGATGCTGTGAAATTCT+TGG 0.430272 5.3:+16467733 MS.gene028111:CDS
TGTGAGAGAAGACATGCTTC+AGG 0.432571 5.3:+16465643 None:intergenic
GCCACAAGCACCCTTAGTTC+TGG 0.432585 5.3:-16467570 None:intergenic
TCATAGGCCCATTCAAGTCC+TGG 0.450896 5.3:-16467824 None:intergenic
AAGTCTCTTCATTTCCTCAT+AGG 0.467730 5.3:-16467840 None:intergenic
CAATATTGATGCCAGAACTA+AGG 0.481725 5.3:+16467559 MS.gene028111:CDS
CTCCACCTTGCTGCCGAAGG+TGG 0.490968 5.3:+16467497 MS.gene028111:CDS
CACTCCGAGATGACCACCTT+CGG 0.497622 5.3:-16467510 None:intergenic
GCTGCCGAAGGTGGTCATCT+CGG 0.507384 5.3:+16467506 MS.gene028111:CDS
TTACTGCCCAGGACTTGAAT+GGG 0.512016 5.3:+16467817 MS.gene028111:CDS
GTGAAATTCTTGGTAGAGAA+TGG 0.513179 5.3:+16467743 MS.gene028111:CDS
CTTCGGCAGCAAGGTGGAGA+GGG 0.515210 5.3:-16467493 None:intergenic
TGCAGCTAAAGAGAGGAATA+GGG 0.519744 5.3:+16467715 MS.gene028111:CDS
AGGACTTGAATGGGCCTATG+AGG 0.531270 5.3:+16467826 MS.gene028111:CDS
CCTCTCCACCTTGCTGCCGA+AGG 0.532516 5.3:+16467494 MS.gene028111:CDS
AATCTGCTATCATTGATATC+CGG 0.533897 5.3:-16467779 None:intergenic
AAAGCTTCTCAACGAAGGAA+TGG 0.535311 5.3:+16467427 MS.gene028111:CDS
TTGATACTGATGATAGAGGT+TGG 0.535680 5.3:+16465723 MS.gene028111:CDS
CCTCCTCTTCATTACTGCCC+AGG 0.539604 5.3:+16467806 MS.gene028111:CDS
AGGTGGTCATCTCGGAGTGA+TGG 0.540638 5.3:+16467514 MS.gene028111:CDS
AATATTGATGCCAGAACTAA+GGG 0.549077 5.3:+16467560 MS.gene028111:CDS
GCATGGGGCCCTAAATCTAA+AGG 0.550158 5.3:+16467464 MS.gene028111:CDS
AAGGAGGTTGTTGAGGCCTG+GGG 0.557016 5.3:+16465700 MS.gene028111:CDS
GAGCTGCAAGATGTGTCCTC+CGG 0.558005 5.3:-16467881 None:intergenic
CATAGGCCCATTCAAGTCCT+GGG 0.560704 5.3:-16467823 None:intergenic
CCTTCGGCAGCAAGGTGGAG+AGG 0.564920 5.3:-16467494 None:intergenic
CTTCATGTTTATGCAAGGAA+AGG 0.570970 5.3:+16465752 MS.gene028111:CDS
AAGGAAAGGTGATATTAAGC+TGG 0.572436 5.3:+16465766 MS.gene028111:CDS
GAATGGAGCATTCTTGCCAC+CGG 0.577208 5.3:+16467760 MS.gene028111:CDS
CCTCGCTTCATGTTTATGCA+AGG 0.580909 5.3:+16465747 MS.gene028111:CDS
TGGATGTTAATGTGACTGCA+TGG 0.583622 5.3:+16467447 MS.gene028111:CDS
CTTCGCGAAGGAGGTTGTTG+AGG 0.590897 5.3:+16465693 MS.gene028111:CDS
TCCATATTGCAGCTAAAGAG+AGG 0.596526 5.3:+16467708 MS.gene028111:CDS
CTATCATCAGTATCAACCCC+AGG 0.598054 5.3:-16465716 None:intergenic
TCGACAGCAAGAATTATCTC+CGG 0.599712 5.3:+16467862 MS.gene028111:CDS
GATGACCACCTTCGGCAGCA+AGG 0.610444 5.3:-16467502 None:intergenic
TTCGGCAGCAAGGTGGAGAG+GGG 0.614770 5.3:-16467492 None:intergenic
GCCAGAACTAAGGGTGCTTG+TGG 0.615169 5.3:+16467569 MS.gene028111:CDS
CTCAACAACCTCCTTCGCGA+AGG 0.617083 5.3:-16465692 None:intergenic
GACAAAGTCCTTCGCGAAGG+AGG 0.622537 5.3:+16465684 MS.gene028111:CDS
AGTCCTGGGCAGTAATGAAG+AGG 0.632969 5.3:-16467809 None:intergenic
GAGAAGACATGCTTCAGGAG+AGG 0.635852 5.3:+16465648 None:intergenic
CCTGGGCAGTAATGAAGAGG+AGG 0.640191 5.3:-16467806 None:intergenic
TCTTTAGCTGCAATATGGAG+CGG 0.641697 5.3:-16467704 None:intergenic
GGATGTTAATGTGACTGCAT+GGG 0.642233 5.3:+16467448 MS.gene028111:CDS
TGCGACAAAGTCCTTCGCGA+AGG 0.643930 5.3:+16465681 MS.gene028111:CDS
CTGCTATCATTGATATCCGG+TGG 0.644744 5.3:-16467776 None:intergenic
GGGGTTGATACTGATGATAG+AGG 0.648484 5.3:+16465719 MS.gene028111:CDS
GATGTTAATGTGACTGCATG+GGG 0.662007 5.3:+16467449 MS.gene028111:CDS
CCTTGCATAAACATGAAGCG+AGG 0.684018 5.3:-16465747 None:intergenic
GACCACCTTCGGCAGCAAGG+TGG 0.692091 5.3:-16467499 None:intergenic
ACAGCAAGAATTATCTCCGG+AGG 0.696504 5.3:+16467865 MS.gene028111:CDS
ATGGATGAACTGCTTGAGCG+TGG 0.700290 5.3:+16467533 MS.gene028111:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATAAAAAAAATCAAAATT+TGG - chr5.3:16467655-16467674 None:intergenic 10.0%
!!! TTTTTTACTATAATATCATT+AGG + chr5.3:16466175-16466194 MS.gene028111:intron 10.0%
!!! ATATGAATGTTTTATTATGA+GGG + chr5.3:16465877-16465896 MS.gene028111:intron 15.0%
!!! TATATGAATGTTTTATTATG+AGG + chr5.3:16465876-16465895 MS.gene028111:intron 15.0%
!! TATAATATCATTAGGAGATA+AGG + chr5.3:16466183-16466202 MS.gene028111:intron 20.0%
!! TTATACTTAAAAATCCTGTT+AGG - chr5.3:16466961-16466980 None:intergenic 20.0%
!!! AATTTTACAATGTGCTATAT+TGG + chr5.3:16466470-16466489 MS.gene028111:intron 20.0%
!!! ATTTTACAATGTGCTATATT+GGG + chr5.3:16466471-16466490 MS.gene028111:intron 20.0%
!!! TGATAATTTTCTACTCTTAA+AGG - chr5.3:16467347-16467366 None:intergenic 20.0%
! AACCCAATAAATCTAAGTAA+AGG - chr5.3:16465964-16465983 None:intergenic 25.0%
! AAGATAAATAATGTGCAAGA+AGG + chr5.3:16465923-16465942 MS.gene028111:intron 25.0%
! AAGTTATGACTAATGAGTTA+AGG + chr5.3:16466707-16466726 MS.gene028111:intron 25.0%
! ACCCAATAAATCTAAGTAAA+GGG - chr5.3:16465963-16465982 None:intergenic 25.0%
! ACTAATATGGAAGTCTATAT+GGG - chr5.3:16467613-16467632 None:intergenic 25.0%
! AGTTATGACTAATGAGTTAA+GGG + chr5.3:16466708-16466727 MS.gene028111:intron 25.0%
! ATGTATTCTCAAAAACATAG+AGG + chr5.3:16466342-16466361 MS.gene028111:intron 25.0%
! GACAGATAGATATACTAATA+TGG - chr5.3:16467626-16467645 None:intergenic 25.0%
! TACTAATATGGAAGTCTATA+TGG - chr5.3:16467614-16467633 None:intergenic 25.0%
! TCCATCTACAAAACAAAATA+TGG - chr5.3:16466091-16466110 None:intergenic 25.0%
! TTTGTCTAATATTCACTTCA+AGG + chr5.3:16466149-16466168 MS.gene028111:intron 25.0%
!! AATTGACTATGTTTTACTTC+TGG + chr5.3:16465822-16465841 MS.gene028111:intron 25.0%
!! TTTTACAATGTGCTATATTG+GGG + chr5.3:16466472-16466491 MS.gene028111:intron 25.0%
!!! ATATTTTGTTTTGTAGATGG+AGG + chr5.3:16466090-16466109 MS.gene028111:intron 25.0%
!!! TATTTTGTTTTGTAGATGGA+GGG + chr5.3:16466091-16466110 MS.gene028111:intron 25.0%
!!! TTATAATGTTGTTTCTGAAC+AGG + chr5.3:16467400-16467419 MS.gene028111:intron 25.0%
AACAAAATATGGCTTTCAGT+AGG - chr5.3:16466080-16466099 None:intergenic 30.0%
AATATTGATGCCAGAACTAA+GGG + chr5.3:16467560-16467579 MS.gene028111:CDS 30.0%
AATCTGCTATCATTGATATC+CGG - chr5.3:16467782-16467801 None:intergenic 30.0%
AGGTTGAATTTGAATAGTAC+AGG + chr5.3:16466406-16466425 MS.gene028111:intron 30.0%
ATTAACAAACAGCCGTTATT+GGG + chr5.3:16467093-16467112 MS.gene028111:intron 30.0%
ATTATCACACTTGAAGTGTA+AGG + chr5.3:16467360-16467379 MS.gene028111:intron 30.0%
GCCCTTTACTTAGATTTATT+GGG + chr5.3:16465959-16465978 MS.gene028111:intron 30.0%
GTACTATTCAAATTCAACCT+AGG - chr5.3:16466406-16466425 None:intergenic 30.0%
TAACTAATTGCAACCATCTA+AGG - chr5.3:16466910-16466929 None:intergenic 30.0%
TAATCCAGCTATATGAATTG+AGG + chr5.3:16466312-16466331 MS.gene028111:intron 30.0%
TGCCCTTTACTTAGATTTAT+TGG + chr5.3:16465958-16465977 MS.gene028111:intron 30.0%
TGGTGAATTTGTAGAAGTAA+CGG + chr5.3:16466742-16466761 MS.gene028111:intron 30.0%
TTAGATGGTTGCAATTAGTT+AGG + chr5.3:16466909-16466928 MS.gene028111:intron 30.0%
TTATCACACTTGAAGTGTAA+GGG + chr5.3:16467361-16467380 MS.gene028111:intron 30.0%
TTCTATACAGCTCTTGTAAA+AGG + chr5.3:16466984-16467003 MS.gene028111:intron 30.0%
! ATTTTGTTCTCCTTAAACCA+GGG + chr5.3:16467678-16467697 MS.gene028111:intron 30.0%
! GCTATCAATCTAGAGTTATT+TGG - chr5.3:16466649-16466668 None:intergenic 30.0%
! GGTCAATTTTCTTGAGATTT+AGG + chr5.3:16466225-16466244 MS.gene028111:intron 30.0%
! TTTTGTAAACACAGCAATGA+AGG - chr5.3:16467224-16467243 None:intergenic 30.0%
!!! GCCATATTTTGTTTTGTAGA+TGG + chr5.3:16466087-16466106 MS.gene028111:intron 30.0%
!!! GGTTAGTTTCTGCATTTTAT+TGG + chr5.3:16466204-16466223 MS.gene028111:intron 30.0%
!!! GTTTTACTTCTGGAATTTTG+TGG + chr5.3:16465832-16465851 MS.gene028111:intron 30.0%
AACTTGCAATTGAAAGTCGT+TGG + chr5.3:16466500-16466519 MS.gene028111:intron 35.0%
AAGGAAAGGTGATATTAAGC+TGG + chr5.3:16465766-16465785 MS.gene028111:CDS 35.0%
AAGTCTCTTCATTTCCTCAT+AGG - chr5.3:16467843-16467862 None:intergenic 35.0%
AATAGTACAGGACTAAAGAG+GGG + chr5.3:16466418-16466437 MS.gene028111:intron 35.0%
ATGAATCTACTGAGAAGGTA+AGG + chr5.3:16466375-16466394 MS.gene028111:intron 35.0%
CAATATTGATGCCAGAACTA+AGG + chr5.3:16467559-16467578 MS.gene028111:CDS 35.0%
CAATCAGTTGCACATTATGT+TGG + chr5.3:16466545-16466564 MS.gene028111:intron 35.0%
CATTAACAAACAGCCGTTAT+TGG + chr5.3:16467092-16467111 MS.gene028111:intron 35.0%
CTTCATGTTTATGCAAGGAA+AGG + chr5.3:16465752-16465771 MS.gene028111:CDS 35.0%
GAATAGTACAGGACTAAAGA+GGG + chr5.3:16466417-16466436 MS.gene028111:intron 35.0%
GGTGAATTTGTAGAAGTAAC+GGG + chr5.3:16466743-16466762 MS.gene028111:intron 35.0%
GTAAAAAAGCTTCTCAACGA+AGG + chr5.3:16467422-16467441 MS.gene028111:CDS 35.0%
GTCTAATATTCACTTCAAGG+TGG + chr5.3:16466152-16466171 MS.gene028111:intron 35.0%
GTGAAATTCTTGGTAGAGAA+TGG + chr5.3:16467743-16467762 MS.gene028111:CDS 35.0%
TACATTTGTAGAGTGTGAAG+TGG + chr5.3:16466816-16466835 MS.gene028111:intron 35.0%
TGAATAGTACAGGACTAAAG+AGG + chr5.3:16466416-16466435 MS.gene028111:intron 35.0%
TGTACCTCAATTCATATAGC+TGG - chr5.3:16466319-16466338 None:intergenic 35.0%
TGTAGATGAATCTACTGAGA+AGG + chr5.3:16466370-16466389 MS.gene028111:intron 35.0%
TGTTGTGTAGGAATTGTGTT+TGG + chr5.3:16466014-16466033 MS.gene028111:intron 35.0%
TTGATACTGATGATAGAGGT+TGG + chr5.3:16465723-16465742 MS.gene028111:CDS 35.0%
! ATCATCGACTAAAGTAAGCA+AGG - chr5.3:16467290-16467309 None:intergenic 35.0%
! ATTGATAGCATAGCATTGAG+AGG + chr5.3:16466660-16466679 MS.gene028111:intron 35.0%
! GATTTTGTTCTCCTTAAACC+AGG + chr5.3:16467677-16467696 MS.gene028111:intron 35.0%
! GCTTCAGTTATGAGATTTTG+GGG + chr5.3:16466045-16466064 MS.gene028111:intron 35.0%
! GGCTTCAGTTATGAGATTTT+GGG + chr5.3:16466044-16466063 MS.gene028111:intron 35.0%
! TGGCTTCAGTTATGAGATTT+TGG + chr5.3:16466043-16466062 MS.gene028111:intron 35.0%
! TTGATAGCATAGCATTGAGA+GGG + chr5.3:16466661-16466680 MS.gene028111:intron 35.0%
!! CTTAGTTCTGGCATCAATAT+TGG - chr5.3:16467561-16467580 None:intergenic 35.0%
!!! GGAGGGTTGTTTTTAACATT+TGG + chr5.3:16466108-16466127 MS.gene028111:intron 35.0%
AAAGCTTCTCAACGAAGGAA+TGG + chr5.3:16467427-16467446 MS.gene028111:CDS 40.0%
AGGGGTTGTTTGTAGATTCA+TGG + chr5.3:16466680-16466699 MS.gene028111:intron 40.0%
CAATTTACCGAAGTGATTGC+TGG + chr5.3:16467031-16467050 MS.gene028111:intron 40.0%
CATAAGACCAGCAATCACTT+CGG - chr5.3:16467041-16467060 None:intergenic 40.0%
CTAGTCTACACCTTCTATGT+TGG - chr5.3:16466843-16466862 None:intergenic 40.0%
GAGAAGGTAAGGTTATTCCT+AGG + chr5.3:16466386-16466405 MS.gene028111:intron 40.0%
GATGTTAATGTGACTGCATG+GGG + chr5.3:16467449-16467468 MS.gene028111:CDS 40.0%
GGATGTTAATGTGACTGCAT+GGG + chr5.3:16467448-16467467 MS.gene028111:CDS 40.0%
GGTACAGTGAAACATTGGAA+GGG + chr5.3:16466521-16466540 MS.gene028111:intron 40.0%
GTCGATGATTCATGAACTGT+TGG + chr5.3:16467301-16467320 MS.gene028111:intron 40.0%
GTTACGCAGTATTGTTCACA+CGG + chr5.3:16466934-16466953 MS.gene028111:intron 40.0%
TAATGAGTTAAGGGTGACCA+GGG + chr5.3:16466717-16466736 MS.gene028111:intron 40.0%
TAGGGATGCTGTGAAATTCT+TGG + chr5.3:16467733-16467752 MS.gene028111:CDS 40.0%
TCCATATTGCAGCTAAAGAG+AGG + chr5.3:16467708-16467727 MS.gene028111:CDS 40.0%
TCCTCTCTTTAGCTGCAATA+TGG - chr5.3:16467712-16467731 None:intergenic 40.0%
TCGACAGCAAGAATTATCTC+CGG + chr5.3:16467862-16467881 MS.gene028111:CDS 40.0%
TCGTACCTCATACCCAATAA+CGG - chr5.3:16467108-16467127 None:intergenic 40.0%
TCGTTGGTACAGTGAAACAT+TGG + chr5.3:16466516-16466535 MS.gene028111:intron 40.0%
TCTTTAGCTGCAATATGGAG+CGG - chr5.3:16467707-16467726 None:intergenic 40.0%
TGCAGCTAAAGAGAGGAATA+GGG + chr5.3:16467715-16467734 MS.gene028111:CDS 40.0%
TGGATGTTAATGTGACTGCA+TGG + chr5.3:16467447-16467466 MS.gene028111:CDS 40.0%
TGGTACAGTGAAACATTGGA+AGG + chr5.3:16466520-16466539 MS.gene028111:intron 40.0%
TTGCAGCTAAAGAGAGGAAT+AGG + chr5.3:16467714-16467733 MS.gene028111:CDS 40.0%
! AAAGGTGCAAGGAATGGTTA+TGG + chr5.3:16467002-16467021 MS.gene028111:intron 40.0%
! CATAGAAGGTGTAGACTAGA+TGG + chr5.3:16466844-16466863 MS.gene028111:intron 40.0%
! CTTGTAAAAGGTGCAAGGAA+TGG + chr5.3:16466996-16467015 MS.gene028111:intron 40.0%
! GATGACAAGTTTGTTTGACG+AGG + chr5.3:16466866-16466885 MS.gene028111:intron 40.0%
! TGATAGCATAGCATTGAGAG+GGG + chr5.3:16466662-16466681 MS.gene028111:intron 40.0%
!! GGAATTGTGTTTGGTTTGAG+TGG + chr5.3:16466023-16466042 MS.gene028111:intron 40.0%
!!! TTTTAGCTCCCAACATGCAT+CGG - chr5.3:16467176-16467195 None:intergenic 40.0%
AACAGCCGTTATTGGGTATG+AGG + chr5.3:16467100-16467119 MS.gene028111:intron 45.0%
AACATGCATCGGGGAATTTG+GGG - chr5.3:16467165-16467184 None:intergenic 45.0%
AAGTCGGTTGCAAGCATCAA+CGG - chr5.3:16467141-16467160 None:intergenic 45.0%
AATGAGTTAAGGGTGACCAG+GGG + chr5.3:16466718-16466737 MS.gene028111:intron 45.0%
ACAACCAGAACTGTGTCAAC+TGG - chr5.3:16467067-16467086 None:intergenic 45.0%
ACAGCAAGAATTATCTCCGG+AGG + chr5.3:16467865-16467884 MS.gene028111:CDS 45.0%
ATTACTGCCCAGGACTTGAA+TGG + chr5.3:16467816-16467835 MS.gene028111:CDS 45.0%
ATTGTTCACACGGACCTAAC+AGG + chr5.3:16466944-16466963 MS.gene028111:intron 45.0%
CAAATTCCCCGATGCATGTT+GGG + chr5.3:16467165-16467184 MS.gene028111:intron 45.0%
CAACATGCATCGGGGAATTT+GGG - chr5.3:16467166-16467185 None:intergenic 45.0%
CCTCGCTTCATGTTTATGCA+AGG + chr5.3:16465747-16465766 MS.gene028111:CDS 45.0%
CCTTGCATAAACATGAAGCG+AGG - chr5.3:16465750-16465769 None:intergenic 45.0%
CTAATGAGTTAAGGGTGACC+AGG + chr5.3:16466716-16466735 MS.gene028111:intron 45.0%
CTATCATCAGTATCAACCCC+AGG - chr5.3:16465719-16465738 None:intergenic 45.0%
CTGCTATCATTGATATCCGG+TGG - chr5.3:16467779-16467798 None:intergenic 45.0%
GACAGTTATGACCAGAAGAG+GGG + chr5.3:16466567-16466586 MS.gene028111:intron 45.0%
GAGATTCGTGTGTGTTGTGT+AGG + chr5.3:16466002-16466021 MS.gene028111:intron 45.0%
GGACAGTTATGACCAGAAGA+GGG + chr5.3:16466566-16466585 MS.gene028111:intron 45.0%
GGACTTTGTCGCAACGAAAT+TGG - chr5.3:16465674-16465693 None:intergenic 45.0%
GGGTCACGCCTTTAGATTTA+GGG - chr5.3:16467475-16467494 None:intergenic 45.0%
GTGAAGTGGTCCAACATAGA+AGG + chr5.3:16466830-16466849 MS.gene028111:intron 45.0%
TAAAGGGTGACTAGTGCACA+AGG - chr5.3:16467199-16467218 None:intergenic 45.0%
TCAGAGTCACACTTCTAGAC+TGG - chr5.3:16466775-16466794 None:intergenic 45.0%
TCTACAAATTCACCAACCCC+TGG - chr5.3:16466737-16466756 None:intergenic 45.0%
TGACTGGCAAGATCCTTAGA+TGG + chr5.3:16466894-16466913 MS.gene028111:intron 45.0%
TGGACAGTTATGACCAGAAG+AGG + chr5.3:16466565-16466584 MS.gene028111:intron 45.0%
TTACTGCCCAGGACTTGAAT+GGG + chr5.3:16467817-16467836 MS.gene028111:CDS 45.0%
! ACACAGCAATGAAGGCGTAA+AGG - chr5.3:16467216-16467235 None:intergenic 45.0%
! CACAGCAATGAAGGCGTAAA+GGG - chr5.3:16467215-16467234 None:intergenic 45.0%
! CAGCTCTTGTAAAAGGTGCA+AGG + chr5.3:16466991-16467010 MS.gene028111:intron 45.0%
! TTTAGCTCCCAACATGCATC+GGG - chr5.3:16467175-16467194 None:intergenic 45.0%
!! GGGGTTGATACTGATGATAG+AGG + chr5.3:16465719-16465738 MS.gene028111:CDS 45.0%
AGGACTTGAATGGGCCTATG+AGG + chr5.3:16467826-16467845 MS.gene028111:CDS 50.0%
AGTCCTGGGCAGTAATGAAG+AGG - chr5.3:16467812-16467831 None:intergenic 50.0%
ATGGATGAACTGCTTGAGCG+TGG + chr5.3:16467533-16467552 MS.gene028111:CDS 50.0%
CATAGGCCCATTCAAGTCCT+GGG - chr5.3:16467826-16467845 None:intergenic 50.0%
CCAAATTCCCCGATGCATGT+TGG + chr5.3:16467164-16467183 MS.gene028111:intron 50.0%
CCAACATGCATCGGGGAATT+TGG - chr5.3:16467167-16467186 None:intergenic 50.0%
GAGACCAGTTGACACAGTTC+TGG + chr5.3:16467060-16467079 MS.gene028111:intron 50.0%
GCATGGGGCCCTAAATCTAA+AGG + chr5.3:16467464-16467483 MS.gene028111:CDS 50.0%
GGGGTCACGCCTTTAGATTT+AGG - chr5.3:16467476-16467495 None:intergenic 50.0%
TCATAGGCCCATTCAAGTCC+TGG - chr5.3:16467827-16467846 None:intergenic 50.0%
TGGGTATGAGGTACGACTGT+AGG + chr5.3:16467112-16467131 MS.gene028111:intron 50.0%
TTAGCTCCCAACATGCATCG+GGG - chr5.3:16467174-16467193 None:intergenic 50.0%
TTATGACCAGAAGAGGGGCT+TGG + chr5.3:16466572-16466591 MS.gene028111:intron 50.0%
! GAATGGAGCATTCTTGCCAC+CGG + chr5.3:16467760-16467779 MS.gene028111:CDS 50.0%
!! ATGTGCAGAGCAGCTCTAGA+TGG + chr5.3:16466609-16466628 MS.gene028111:intron 50.0%
!! TGTGCAGAGCAGCTCTAGAT+GGG + chr5.3:16466610-16466629 MS.gene028111:intron 50.0%
AAGGAGGTTGTTGAGGCCTG+GGG + chr5.3:16465700-16465719 MS.gene028111:CDS 55.0%
AGGTGGTCATCTCGGAGTGA+TGG + chr5.3:16467514-16467533 MS.gene028111:CDS 55.0%
AGTTAAGGGTGACCAGGGGT+TGG + chr5.3:16466722-16466741 MS.gene028111:intron 55.0%
ATACTGCCAAGCCCCTCTTC+TGG - chr5.3:16466581-16466600 None:intergenic 55.0%
CACTCCGAGATGACCACCTT+CGG - chr5.3:16467513-16467532 None:intergenic 55.0%
CCTCCTCTTCATTACTGCCC+AGG + chr5.3:16467806-16467825 MS.gene028111:CDS 55.0%
CCTGGGCAGTAATGAAGAGG+AGG - chr5.3:16467809-16467828 None:intergenic 55.0%
CTCAACAACCTCCTTCGCGA+AGG - chr5.3:16465695-16465714 None:intergenic 55.0%
CTTCGCGAAGGAGGTTGTTG+AGG + chr5.3:16465693-16465712 MS.gene028111:CDS 55.0%
GAAGGAGGTTGTTGAGGCCT+GGG + chr5.3:16465699-16465718 MS.gene028111:CDS 55.0%
GACAAAGTCCTTCGCGAAGG+AGG + chr5.3:16465684-16465703 MS.gene028111:CDS 55.0%
GAGCTGCAAGATGTGTCCTC+CGG - chr5.3:16467884-16467903 None:intergenic 55.0%
GCCACAAGCACCCTTAGTTC+TGG - chr5.3:16467573-16467592 None:intergenic 55.0%
GTACAGGACTAAAGAGGGGC+AGG + chr5.3:16466422-16466441 MS.gene028111:intron 55.0%
GTTTGACGAGGTTGCGTGAC+TGG + chr5.3:16466878-16466897 MS.gene028111:intron 55.0%
TGCGACAAAGTCCTTCGCGA+AGG + chr5.3:16465681-16465700 MS.gene028111:CDS 55.0%
! TCGGGGAATTTGGGGCAAGT+CGG - chr5.3:16467157-16467176 None:intergenic 55.0%
!! GCCAGAACTAAGGGTGCTTG+TGG + chr5.3:16467569-16467588 MS.gene028111:CDS 55.0%
!! GTGCAGAGCAGCTCTAGATG+GGG + chr5.3:16466611-16466630 MS.gene028111:intron 55.0%
CGAAGGAGGTTGTTGAGGCC+TGG + chr5.3:16465698-16465717 MS.gene028111:CDS 60.0%
CTTCGGCAGCAAGGTGGAGA+GGG - chr5.3:16467496-16467515 None:intergenic 60.0%
GATGACCACCTTCGGCAGCA+AGG - chr5.3:16467505-16467524 None:intergenic 60.0%
GCAATATGGAGCGGTGTCCC+TGG - chr5.3:16467698-16467717 None:intergenic 60.0%
GCTGCCGAAGGTGGTCATCT+CGG + chr5.3:16467506-16467525 MS.gene028111:CDS 60.0%
GGAGCGGTGTCCCTGGTTTA+AGG - chr5.3:16467691-16467710 None:intergenic 60.0%
TTCGGCAGCAAGGTGGAGAG+GGG - chr5.3:16467495-16467514 None:intergenic 60.0%
CCTCTCCACCTTGCTGCCGA+AGG + chr5.3:16467494-16467513 MS.gene028111:CDS 65.0%
CCTTCGGCAGCAAGGTGGAG+AGG - chr5.3:16467497-16467516 None:intergenic 65.0%
CTCCACCTTGCTGCCGAAGG+TGG + chr5.3:16467497-16467516 MS.gene028111:CDS 65.0%
GACCACCTTCGGCAGCAAGG+TGG - chr5.3:16467502-16467521 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 16465656 16467913 16465656 ID=MS.gene028111
chr5.3 mRNA 16465656 16467913 16465656 ID=MS.gene028111.t1;Parent=MS.gene028111
chr5.3 exon 16465656 16465787 16465656 ID=MS.gene028111.t1.exon1;Parent=MS.gene028111.t1
chr5.3 CDS 16465656 16465787 16465656 ID=cds.MS.gene028111.t1;Parent=MS.gene028111.t1
chr5.3 exon 16467422 16467593 16467422 ID=MS.gene028111.t1.exon2;Parent=MS.gene028111.t1
chr5.3 CDS 16467422 16467593 16467422 ID=cds.MS.gene028111.t1;Parent=MS.gene028111.t1
chr5.3 exon 16467699 16467913 16467699 ID=MS.gene028111.t1.exon3;Parent=MS.gene028111.t1
chr5.3 CDS 16467699 16467913 16467699 ID=cds.MS.gene028111.t1;Parent=MS.gene028111.t1
Gene Sequence

>MS.gene028111

ATGCTTCAGGAGAGGCCAATTTCGTTGCGACAAAGTCCTTCGCGAAGGAGGTTGTTGAGGCCTGGGGTTGATACTGATGATAGAGGTTGGACCTCGCTTCATGTTTATGCAAGGAAAGGTGATATTAAGCTGGTAAGTTCTTTTCTTTTGTTCATTTTTTTATGCAAATTGACTATGTTTTACTTCTGGAATTTTGTGGATAAAATTCGAGAAATGTATGTATATGAATGTTTTATTATGAGGGTAAGTTTGATTTTGACGTGCACAAAGATAAATAATGTGCAAGAAGGAAAACTCAAGTGTGCCCTTTACTTAGATTTATTGGGTTGATTGAATTTGAGATATAGAGATTCGTGTGTGTTGTGTAGGAATTGTGTTTGGTTTGAGTGGCTTCAGTTATGAGATTTTGGGGCTTTTGTTTCCTACTGAAAGCCATATTTTGTTTTGTAGATGGAGGGTTGTTTTTAACATTTGGTTACATCTCTATCATATATTTGTCTAATATTCACTTCAAGGTGGTTTTTTACTATAATATCATTAGGAGATAAGGTTAGTTTCTGCATTTTATTGGTCAATTTTCTTGAGATTTAGGTGTGTAGCATATCTCTCAACTTTTTTATCACATAATCGAGCTATATGAAATGAGTTTTGTCACATAATCCAGCTATATGAATTGAGGTACACATATGTATTCTCAAAAACATAGAGGCTCAATGTAGATGAATCTACTGAGAAGGTAAGGTTATTCCTAGGTTGAATTTGAATAGTACAGGACTAAAGAGGGGCAGGACAGTATGCAAACAATGTATAGAACAATTTTACAATGTGCTATATTGGGGCAAATAACTTGCAATTGAAAGTCGTTGGTACAGTGAAACATTGGAAGGGACAATCAGTTGCACATTATGTTGGACAGTTATGACCAGAAGAGGGGCTTGGCAGTATGTCAAACAATGTGCAGAGCAGCTCTAGATGGGGCTATGTGTATTGCCAAATAACTCTAGATTGATAGCATAGCATTGAGAGGGGTTGTTTGTAGATTCATGGTCAGAAGTTATGACTAATGAGTTAAGGGTGACCAGGGGTTGGTGAATTTGTAGAAGTAACGGGTATTCTCCAGTCTAGAAGTGTGACTCTGATTCTGTCAGACATGTAGTATATACATTTGTAGAGTGTGAAGTGGTCCAACATAGAAGGTGTAGACTAGATGGATGACAAGTTTGTTTGACGAGGTTGCGTGACTGGCAAGATCCTTAGATGGTTGCAATTAGTTAGGAAGTTACGCAGTATTGTTCACACGGACCTAACAGGATTTTTAAGTATAAATGTTCTATACAGCTCTTGTAAAAGGTGCAAGGAATGGTTATGGTTACTGCAATTTACCGAAGTGATTGCTGGTCTTATGAGACCAGTTGACACAGTTCTGGTTGTGTAAACATTAACAAACAGCCGTTATTGGGTATGAGGTACGACTGTAGGCAACCGTTGATGCTTGCAACCGACTTGCCCCAAATTCCCCGATGCATGTTGGGAGCTAAAACCTTGTGCACTAGTCACCCTTTACGCCTTCATTGCTGTGTTTACAAAATATTCAAACTTTTTGCTACTAATTTCTGTTGTTTAATCTTTTTCCTTGCTTACTTTAGTCGATGATTCATGAACTGTTGGATTATATACGATTTATTATTCCTTTAAGAGTAGAAAATTATCACACTTGAAGTGTAAGGGAAGCATCTGCTTATATTTATAATGTTGTTTCTGAACAGGTAAAAAAGCTTCTCAACGAAGGAATGGATGTTAATGTGACTGCATGGGGCCCTAAATCTAAAGGCGTGACCCCTCTCCACCTTGCTGCCGAAGGTGGTCATCTCGGAGTGATGGATGAACTGCTTGAGCGTGGTGCCAATATTGATGCCAGAACTAAGGGTGCTTGTGGCTGTAAGTTTTTATGCTTCCCATATAGACTTCCATATTAGTATATCTATCTGTCTAAATTCCAAATTTTGATTTTTTTTATTCATGATTTTGTTCTCCTTAAACCAGGGACACCGCTCCATATTGCAGCTAAAGAGAGGAATAGGGATGCTGTGAAATTCTTGGTAGAGAATGGAGCATTCTTGCCACCGGATATCAATGATAGCAGATTTAATCCTCCTCTTCATTACTGCCCAGGACTTGAATGGGCCTATGAGGAAATGAAGAGACTTCGACAGCAAGAATTATCTCCGGAGGACACATCTTGCAGCTCTGAAAATTAA
Protein sequence

>MS.gene028111.t1

MLQERPISLRQSPSRRRLLRPGVDTDDRGWTSLHVYARKGDIKLVKKLLNEGMDVNVTAWGPKSKGVTPLHLAAEGGHLGVMDELLERGANIDARTKGACGWTPLHIAAKERNRDAVKFLVENGAFLPPDINDSRFNPPLHYCPGLEWAYEEMKRLRQQELSPEDTSCSSEN