Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009530.t1 | RHN60700.1 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 4.30E-51 | 211.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009530.t1 | Q9H6L4 | 64.3 | 56 | 20 | 0 | 19 | 74 | 13 | 68 | 4.0e-13 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009530.t1 | A0A396I501 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 3.1e-51 | 211.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00474 | MS.gene009530 | PPI |
| MS.gene009530 | MS.gene24450 | PPI |
| MS.gene009530 | MS.gene044085 | PPI |
| MS.gene009530 | MS.gene00478 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009530.t1 | MTR_5g077040 | 98.315 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 2.97e-128 | 357 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009530.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 4.60e-99 | 283 |
| MS.gene009530.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 4.60e-99 | 283 |
| MS.gene009530.t1 | AT5G37290 | 71.311 | 122 | 35 | 0 | 1 | 122 | 1 | 122 | 1.30e-65 | 197 |
Find 42 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTGCCTTTGGTTAT+TGG | 0.174361 | 5.3:-62596679 | None:intergenic |
| AATGAAAAGATTATAGAATT+TGG | 0.211644 | 5.3:+62597214 | MS.gene009530:CDS |
| AGAAATTAAAGAGAAGATTT+TGG | 0.229064 | 5.3:+62596989 | MS.gene009530:intron |
| ATAATTGCAGCTAAATGTTT+TGG | 0.267268 | 5.3:+62597162 | MS.gene009530:intron |
| ATCCTTACAGTGTTCCTAAC+TGG | 0.299126 | 5.3:-62597427 | None:intergenic |
| CGGTCCGCTCTTGTTGCCTT+TGG | 0.333981 | 5.3:-62596686 | None:intergenic |
| GTTAAAATTGTAAGGATCAT+AGG | 0.342362 | 5.3:-62597030 | None:intergenic |
| TAGTGTGAGCTTCAGTAATC+TGG | 0.391697 | 5.3:+62597806 | MS.gene009530:CDS |
| CAAGAGCGGACCGGAAGATA+TGG | 0.397319 | 5.3:+62596696 | MS.gene009530:CDS |
| CACCTGAAGGTATTGCAATC+TGG | 0.406154 | 5.3:-62596724 | None:intergenic |
| AATGTTTATCAAGCCCAGTT+AGG | 0.407380 | 5.3:+62597413 | MS.gene009530:CDS |
| CGCAAGAAGTTAAAATTGTA+AGG | 0.419936 | 5.3:-62597038 | None:intergenic |
| CCTTTGGTTATTGGTGAACA+TGG | 0.442442 | 5.3:-62596670 | None:intergenic |
| TTGATGACATCAATAACTTC+AGG | 0.448267 | 5.3:-62597759 | None:intergenic |
| TTCCAGATTGCAATACCTTC+AGG | 0.449232 | 5.3:+62596722 | MS.gene009530:CDS |
| GATAAACATTGAATGATAAG+AGG | 0.452418 | 5.3:-62597400 | None:intergenic |
| ATTATAGAATTTGGTGTTGG+AGG | 0.454107 | 5.3:+62597223 | MS.gene009530:CDS |
| GCTACAATTGTAGCGTTGGC+TGG | 0.465004 | 5.3:-62597361 | None:intergenic |
| TCCTTACAGTGTTCCTAACT+GGG | 0.468459 | 5.3:-62597426 | None:intergenic |
| AAGATTATAGAATTTGGTGT+TGG | 0.481352 | 5.3:+62597220 | MS.gene009530:CDS |
| TTCTTGACAAACATCTATCA+AGG | 0.487090 | 5.3:+62597838 | MS.gene009530:CDS |
| TTATAGAATTTGGTGTTGGA+GGG | 0.493970 | 5.3:+62597224 | MS.gene009530:CDS |
| ACAATTGTAGCTGGAGTTGG+TGG | 0.514415 | 5.3:+62597373 | MS.gene009530:CDS |
| TCTGGAAGTACCATATCTTC+CGG | 0.518139 | 5.3:-62596706 | None:intergenic |
| GCCAACGCTACAATTGTAGC+TGG | 0.526233 | 5.3:+62597364 | MS.gene009530:CDS |
| TCCAGCTACAATTGTAGCGT+TGG | 0.532247 | 5.3:-62597365 | None:intergenic |
| GTAAGGATCATAGGCAAAGT+TGG | 0.532617 | 5.3:-62597021 | None:intergenic |
| ATCAGGTAAATTATGCACTC+GGG | 0.532999 | 5.3:+62597688 | MS.gene009530:intron |
| CAAAGGCAACAAGAGCGGAC+CGG | 0.544877 | 5.3:+62596687 | MS.gene009530:CDS |
| GATCAGGTAAATTATGCACT+CGG | 0.549275 | 5.3:+62597687 | MS.gene009530:intron |
| GCTACAATTGTAGCTGGAGT+TGG | 0.558809 | 5.3:+62597370 | MS.gene009530:CDS |
| TAATGAATCTAACAAGGAAG+AGG | 0.565289 | 5.3:+62597728 | MS.gene009530:CDS |
| TGGGTTCAGTTAAGCAGTCA+AGG | 0.565826 | 5.3:-62597192 | None:intergenic |
| GGGATCTGCAATTGTTGTGT+TGG | 0.566984 | 5.3:+62597244 | MS.gene009530:CDS |
| TTTCAGAATTCATCAGAGGA+TGG | 0.593173 | 5.3:+62596849 | MS.gene009530:CDS |
| CAATTGTAGCTGGAGTTGGT+GGG | 0.606878 | 5.3:+62597374 | MS.gene009530:CDS |
| CATCTGTAATGAATCTAACA+AGG | 0.607086 | 5.3:+62597722 | MS.gene009530:CDS |
| AAGTTATTGATGTCATCAAG+AGG | 0.636725 | 5.3:+62597763 | MS.gene009530:CDS |
| TCAGTTTCAGAATTCATCAG+AGG | 0.641335 | 5.3:+62596845 | MS.gene009530:CDS |
| CCATGTTCACCAATAACCAA+AGG | 0.659452 | 5.3:+62596670 | None:intergenic |
| ATAACCAAAGGCAACAAGAG+CGG | 0.667897 | 5.3:+62596682 | MS.gene009530:CDS |
| TCAGGTAAATTATGCACTCG+GGG | 0.677160 | 5.3:+62597689 | MS.gene009530:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAAGATTATAGAATT+TGG | + | chr5.3:62597214-62597233 | MS.gene009530:CDS | 15.0% |
| !!! | AAATTCTATAATCTTTTCAT+TGG | - | chr5.3:62597215-62597234 | None:intergenic | 15.0% |
| !!! | AATTCTATAATCTTTTCATT+GGG | - | chr5.3:62597214-62597233 | None:intergenic | 15.0% |
| !!! | AGTTGTTTATCAAATTTTTA+GGG | - | chr5.3:62597654-62597673 | None:intergenic | 15.0% |
| !!! | TAGTTGTTTATCAAATTTTT+AGG | - | chr5.3:62597655-62597674 | None:intergenic | 15.0% |
| !! | AAACAAACGTTATTCATATA+AGG | - | chr5.3:62596811-62596830 | None:intergenic | 20.0% |
| !! | ATGCAAATTAAGTAGTATAA+GGG | - | chr5.3:62597526-62597545 | None:intergenic | 20.0% |
| !!! | AGAAATTAAAGAGAAGATTT+TGG | + | chr5.3:62596989-62597008 | MS.gene009530:intron | 20.0% |
| !!! | GTTGTTTATCAAATTTTTAG+GGG | - | chr5.3:62597653-62597672 | None:intergenic | 20.0% |
| ! | ACTAATGTATTTATCTGATC+AGG | + | chr5.3:62597671-62597690 | MS.gene009530:intron | 25.0% |
| ! | ATATGAATAACGTTTGTTTC+AGG | + | chr5.3:62596812-62596831 | MS.gene009530:intron | 25.0% |
| ! | GATAAACATTGAATGATAAG+AGG | - | chr5.3:62597403-62597422 | None:intergenic | 25.0% |
| ! | GATGCAAATTAAGTAGTATA+AGG | - | chr5.3:62597527-62597546 | None:intergenic | 25.0% |
| ! | GTTAAAATTGTAAGGATCAT+AGG | - | chr5.3:62597033-62597052 | None:intergenic | 25.0% |
| ! | TTATTGCAAAGACAATTAGT+GGG | - | chr5.3:62597075-62597094 | None:intergenic | 25.0% |
| !! | ATAATTGCAGCTAAATGTTT+TGG | + | chr5.3:62597162-62597181 | MS.gene009530:intron | 25.0% |
| !!! | AAGATTATAGAATTTGGTGT+TGG | + | chr5.3:62597220-62597239 | MS.gene009530:CDS | 25.0% |
| AAGTTATTGATGTCATCAAG+AGG | + | chr5.3:62597763-62597782 | MS.gene009530:CDS | 30.0% | |
| AGATAGAAATCACAATACTG+TGG | - | chr5.3:62597291-62597310 | None:intergenic | 30.0% | |
| ATAAAGAATCCTACCAACAA+AGG | - | chr5.3:62596924-62596943 | None:intergenic | 30.0% | |
| ATAACGTTTGTTTCAGGAAT+TGG | + | chr5.3:62596818-62596837 | MS.gene009530:intron | 30.0% | |
| CATCTGTAATGAATCTAACA+AGG | + | chr5.3:62597722-62597741 | MS.gene009530:CDS | 30.0% | |
| CGCAAGAAGTTAAAATTGTA+AGG | - | chr5.3:62597041-62597060 | None:intergenic | 30.0% | |
| CTTATTGCAAAGACAATTAG+TGG | - | chr5.3:62597076-62597095 | None:intergenic | 30.0% | |
| TAATGAATCTAACAAGGAAG+AGG | + | chr5.3:62597728-62597747 | MS.gene009530:CDS | 30.0% | |
| TTCTTGACAAACATCTATCA+AGG | + | chr5.3:62597838-62597857 | MS.gene009530:CDS | 30.0% | |
| ! | TTGATGACATCAATAACTTC+AGG | - | chr5.3:62597762-62597781 | None:intergenic | 30.0% |
| ! | TTTACCATCTCCTTATTTGT+TGG | + | chr5.3:62596945-62596964 | MS.gene009530:intron | 30.0% |
| !! | AATTTTTAGGGGATCTTTTG+TGG | - | chr5.3:62597642-62597661 | None:intergenic | 30.0% |
| !! | AGAGAAGATTTTGGCAAATT+TGG | + | chr5.3:62596998-62597017 | MS.gene009530:CDS | 30.0% |
| !! | ATTATAGAATTTGGTGTTGG+AGG | + | chr5.3:62597223-62597242 | MS.gene009530:CDS | 30.0% |
| !! | TTATAGAATTTGGTGTTGGA+GGG | + | chr5.3:62597224-62597243 | MS.gene009530:CDS | 30.0% |
| !!! | GTTTGCTTGTTGATTTTGTA+AGG | + | chr5.3:62597454-62597473 | MS.gene009530:intron | 30.0% |
| AACACCAACAAATAAGGAGA+TGG | - | chr5.3:62596952-62596971 | None:intergenic | 35.0% | |
| AATCTTTCAAGCACCTTTGT+TGG | + | chr5.3:62596908-62596927 | MS.gene009530:intron | 35.0% | |
| AATGTTTATCAAGCCCAGTT+AGG | + | chr5.3:62597413-62597432 | MS.gene009530:CDS | 35.0% | |
| ACGAGAAACACCAACAAATA+AGG | - | chr5.3:62596958-62596977 | None:intergenic | 35.0% | |
| AGAAATCACAATACTGTGGA+AGG | - | chr5.3:62597287-62597306 | None:intergenic | 35.0% | |
| ATCAGGTAAATTATGCACTC+GGG | + | chr5.3:62597688-62597707 | MS.gene009530:intron | 35.0% | |
| GAAGAAGAAAAAACACCTGA+AGG | - | chr5.3:62596740-62596759 | None:intergenic | 35.0% | |
| GATCAGGTAAATTATGCACT+CGG | + | chr5.3:62597687-62597706 | MS.gene009530:intron | 35.0% | |
| TCAGTTTCAGAATTCATCAG+AGG | + | chr5.3:62596845-62596864 | MS.gene009530:CDS | 35.0% | |
| TTTCAGAATTCATCAGAGGA+TGG | + | chr5.3:62596849-62596868 | MS.gene009530:CDS | 35.0% | |
| !! | ACAAGCGATTTTGAGAAATC+AGG | - | chr5.3:62597557-62597576 | None:intergenic | 35.0% |
| ATAACCAAAGGCAACAAGAG+CGG | + | chr5.3:62596682-62596701 | MS.gene009530:CDS | 40.0% | |
| ATCCTTACAGTGTTCCTAAC+TGG | - | chr5.3:62597430-62597449 | None:intergenic | 40.0% | |
| CTCTTGTTGCCTTTGGTTAT+TGG | - | chr5.3:62596682-62596701 | None:intergenic | 40.0% | |
| GTAAGGATCATAGGCAAAGT+TGG | - | chr5.3:62597024-62597043 | None:intergenic | 40.0% | |
| TAGTGTGAGCTTCAGTAATC+TGG | + | chr5.3:62597806-62597825 | MS.gene009530:CDS | 40.0% | |
| TCAGGTAAATTATGCACTCG+GGG | + | chr5.3:62597689-62597708 | MS.gene009530:intron | 40.0% | |
| TCCTTACAGTGTTCCTAACT+GGG | - | chr5.3:62597429-62597448 | None:intergenic | 40.0% | |
| TTCCAGATTGCAATACCTTC+AGG | + | chr5.3:62596722-62596741 | MS.gene009530:CDS | 40.0% | |
| TTTCAAGCACCTTTGTTGGT+AGG | + | chr5.3:62596912-62596931 | MS.gene009530:intron | 40.0% | |
| !! | TCTGGAAGTACCATATCTTC+CGG | - | chr5.3:62596709-62596728 | None:intergenic | 40.0% |
| !!! | CAATTTTAACTTCTTGCGCC+AGG | + | chr5.3:62597043-62597062 | MS.gene009530:CDS | 40.0% |
| ACAATTGTAGCTGGAGTTGG+TGG | + | chr5.3:62597373-62597392 | MS.gene009530:CDS | 45.0% | |
| GCTACAATTGTAGCTGGAGT+TGG | + | chr5.3:62597370-62597389 | MS.gene009530:CDS | 45.0% | |
| GGGATCTGCAATTGTTGTGT+TGG | + | chr5.3:62597244-62597263 | MS.gene009530:CDS | 45.0% | |
| TCCAGCTACAATTGTAGCGT+TGG | - | chr5.3:62597368-62597387 | None:intergenic | 45.0% | |
| TGGGTTCAGTTAAGCAGTCA+AGG | - | chr5.3:62597195-62597214 | None:intergenic | 45.0% | |
| TTAACTTCTTGCGCCAGGTA+TGG | + | chr5.3:62597048-62597067 | MS.gene009530:intron | 45.0% | |
| ! | ACAATTAGTGGGTCCATACC+TGG | - | chr5.3:62597064-62597083 | None:intergenic | 45.0% |
| ! | CAATTGTAGCTGGAGTTGGT+GGG | + | chr5.3:62597374-62597393 | MS.gene009530:CDS | 45.0% |
| !! | CACCTGAAGGTATTGCAATC+TGG | - | chr5.3:62596727-62596746 | None:intergenic | 45.0% |
| GCCAACGCTACAATTGTAGC+TGG | + | chr5.3:62597364-62597383 | MS.gene009530:CDS | 50.0% | |
| GCCCAGTTAGGAACACTGTA+AGG | + | chr5.3:62597425-62597444 | MS.gene009530:intron | 50.0% | |
| !! | GCTACAATTGTAGCGTTGGC+TGG | - | chr5.3:62597364-62597383 | None:intergenic | 50.0% |
| CAAAGGCAACAAGAGCGGAC+CGG | + | chr5.3:62596687-62596706 | MS.gene009530:CDS | 55.0% | |
| CAAGAGCGGACCGGAAGATA+TGG | + | chr5.3:62596696-62596715 | MS.gene009530:CDS | 55.0% | |
| CGGTCCGCTCTTGTTGCCTT+TGG | - | chr5.3:62596689-62596708 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 62596672 | 62597869 | 62596672 | ID=MS.gene009530 |
| chr5.3 | mRNA | 62596672 | 62597869 | 62596672 | ID=MS.gene009530.t1;Parent=MS.gene009530 |
| chr5.3 | exon | 62596672 | 62596743 | 62596672 | ID=MS.gene009530.t1.exon1;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62596672 | 62596743 | 62596672 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| chr5.3 | exon | 62596834 | 62596870 | 62596834 | ID=MS.gene009530.t1.exon2;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62596834 | 62596870 | 62596834 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| chr5.3 | exon | 62596991 | 62597064 | 62596991 | ID=MS.gene009530.t1.exon3;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62596991 | 62597064 | 62596991 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| chr5.3 | exon | 62597172 | 62597265 | 62597172 | ID=MS.gene009530.t1.exon4;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62597172 | 62597265 | 62597172 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| chr5.3 | exon | 62597359 | 62597441 | 62597359 | ID=MS.gene009530.t1.exon5;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62597359 | 62597441 | 62597359 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| chr5.3 | exon | 62597693 | 62597869 | 62597693 | ID=MS.gene009530.t1.exon6;Parent=MS.gene009530.t1 |
| chr5.3 | CDS | 62597693 | 62597869 | 62597693 | ID=cds.MS.gene009530.t1;Parent=MS.gene009530.t1 |
| Gene Sequence |
| Protein sequence |
