AlfalfaGEDB | MS.gene00474

M. sativa cultivar XinJiangDaYe / MS.gene00474

KEGG: K06627
GO: GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0110165
PFAM: PF00134.26 PF02984.22

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00474.t1	XP_024636078.1	93.4	364	20	1	1	360	1	364	5.40E-186	660.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00474.t1	Q9FMH5	59.0	356	139	4	1	354	1	351	2.1e-109	397.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00474.t1	A0A396JGA0	93.4	364	20	1	1	360	1	364	3.9e-186	660.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene001968	MS.gene00474	PPI
MS.gene00474	MS.gene88440	PPI
MS.gene00474	MS.gene021632	PPI
MS.gene00474	MS.gene063627	PPI
MS.gene00474	MS.gene014786	PPI
MS.gene00474	MS.gene015942	PPI
MS.gene00474	MS.gene009530	PPI
MS.gene00474	MS.gene044085	PPI
MS.gene00474	MS.gene021870	PPI
MS.gene00474	MS.gene072954	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00474.t1	MTR_1g018680	94.536	366	11	3	1	360	1	363	0.0	675
MS.gene00474.t1	MTR_3g102520	55.917	338	133	5	30	354	23	357	2.94e-126	367
MS.gene00474.t1	MTR_3g102530	54.755	347	145	4	16	353	6	349	6.16e-123	358
MS.gene00474.t1	MTR_3g102510	53.988	326	139	5	30	354	23	338	2.79e-116	341
MS.gene00474.t1	MTR_3g102560	53.824	340	137	5	14	353	5	324	2.71e-115	338
MS.gene00474.t1	MTR_3g088415	53.597	278	124	3	78	354	231	504	2.12e-104	316
MS.gene00474.t1	MTR_3g102540	55.039	258	109	2	91	348	13	263	6.67e-97	289
MS.gene00474.t1	MTR_3g088405	49.281	278	136	3	79	354	235	509	2.47e-94	290
MS.gene00474.t1	MTR_3g107970	51.273	275	131	2	76	350	202	473	1.32e-92	285
MS.gene00474.t1	MTR_2g102520	51.111	270	127	3	78	345	206	472	3.43e-92	284

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00474.t1	AT5G43080	59.714	350	134	4	7	354	7	351	2.44e-146	418
MS.gene00474.t1	AT1G47230	58.172	361	133	6	7	354	6	361	7.57e-144	412
MS.gene00474.t1	AT5G43080	58.857	350	132	5	7	354	7	346	2.75e-142	407
MS.gene00474.t1	AT1G47230	58.011	362	133	7	7	354	6	362	4.12e-142	407
MS.gene00474.t1	AT1G47210	56.303	357	135	5	16	354	15	368	1.83e-137	396
MS.gene00474.t1	AT1G47220	56.711	298	120	2	57	354	34	322	1.87e-116	341
MS.gene00474.t1	AT1G44110	54.545	275	120	3	80	353	187	457	3.21e-102	309
MS.gene00474.t1	AT5G25380	50.719	278	126	3	79	352	165	435	7.36e-97	295
MS.gene00474.t1	AT5G25380	50.719	278	126	3	79	352	165	435	7.36e-97	295
MS.gene00474.t1	AT5G25380	50.719	278	126	3	79	352	171	441	1.27e-96	294

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Find 70 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAGAGATATCTCCG+GGG	0.745816	1.4:+4568950	None:intergenic
AAACGAAAACGTGTTCCTCT+CGG	0.594016	1.4:-4571442	MS.gene00474:CDS
AAAGAAAGAGATATCTCCGG+GGG	0.686776	1.4:+4568951	None:intergenic
AACTCCGGTACCGGAGAAAT+TGG	0.300734	1.4:-4571326	MS.gene00474:CDS
AAGAGCCGCCTTTCCTACCA+AGG	0.647189	1.4:+4569194	None:intergenic
AAGCCCGAAGACTCATCCTT+GGG	0.535304	1.4:-4569373	MS.gene00474:intron
AAGTCATTGAAGTTTGAATT+GGG	0.468127	1.4:-4570366	MS.gene00474:CDS
AATATACATGACTTGTACCT+TGG	0.507114	1.4:-4569211	MS.gene00474:CDS
ACACATTCCTTCAAGTCTGC+AGG	0.460260	1.4:+4569238	None:intergenic
ACATAAGTTTCACATAACTG+AGG	0.720028	1.4:+4571289	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGAAAGAGATATCTCC+GGG	+	chr1.4:4571506-4571525	None:intergenic	30.0%
AAAAAGTTAGTAAGGTTGCA+TGG	+	chr1.4:4570655-4570674	None:intergenic	30.0%
AAAAGTTAGTAAGGTTGCAT+GGG	+	chr1.4:4570654-4570673	None:intergenic	30.0%
AACCGAAAAATAAAGCGTAT+CGG	+	chr1.4:4569462-4569481	None:intergenic	30.0%
AACCTATAACTACTATGAGA+TGG	+	chr1.4:4570293-4570312	None:intergenic	30.0%
AATTTGGGTGCAAACAAATT+GGG	+	chr1.4:4569642-4569661	None:intergenic	30.0%
ACATAAGTTTCACATAACTG+AGG	+	chr1.4:4569166-4569185	None:intergenic	30.0%
ACCTATAACTACTATGAGAT+GGG	+	chr1.4:4570292-4570311	None:intergenic	30.0%
ACGAATTAAAACATTACCCA+AGG	+	chr1.4:4571098-4571117	None:intergenic	30.0%
AGAAGATTCATCACTAAAGT+TGG	-	chr1.4:4570229-4570248	MS.gene00474:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	4568923	4571551	4568923	ID=MS.gene00474
chr1.4	mRNA	4568923	4571551	4568923	ID=MS.gene00474.t1;Parent=MS.gene00474
chr1.4	exon	4571255	4571551	4571255	ID=MS.gene00474.t1.exon1;Parent=MS.gene00474.t1
chr1.4	CDS	4571255	4571551	4571255	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4570907	4571139	4570907	ID=MS.gene00474.t1.exon2;Parent=MS.gene00474.t1
chr1.4	CDS	4570907	4571139	4570907	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4570522	4570597	4570522	ID=MS.gene00474.t1.exon3;Parent=MS.gene00474.t1
chr1.4	CDS	4570522	4570597	4570522	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4570339	4570415	4570339	ID=MS.gene00474.t1.exon4;Parent=MS.gene00474.t1
chr1.4	CDS	4570339	4570415	4570339	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4570207	4570243	4570207	ID=MS.gene00474.t1.exon5;Parent=MS.gene00474.t1
chr1.4	CDS	4570207	4570243	4570207	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4569374	4569523	4569374	ID=MS.gene00474.t1.exon6;Parent=MS.gene00474.t1
chr1.4	CDS	4569374	4569523	4569374	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4569159	4569293	4569159	ID=MS.gene00474.t1.exon7;Parent=MS.gene00474.t1
chr1.4	CDS	4569159	4569293	4569159	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1
chr1.4	exon	4568923	4569000	4568923	ID=MS.gene00474.t1.exon8;Parent=MS.gene00474.t1
chr1.4	CDS	4568923	4569000	4568923	ID=cds.MS.gene00474.t1;Parent=MS.gene00474.t1

Gene Sequence

>MS.gene00474

ATGGAACCAAAGAGCGTTAACCCCACGCGCCGCCCTCAACGTGAAGCAAAGAAAAAAGCCGTAGCTGCACTATGTGAACAACAACGCAAACGAAAACGTGTTCCTCTCGGTGAAATAACAAACGACGTAATCACAAACGCAAACGATGTCGTTTTACCAACGCAGAAAAAACGAAACGTTGCTACTTCATCAAACTCCGATGAAACTCCGGTACCGGAGAAATTGGAGAAATTCGAAGATCCTCAGTTATGTGAAACTTATGTTTCTGATATTTACGATTATCTTCGTAACATGGAGGTAATTTGATTTACTTAATTTAACTTCAAATTTCAATTAGGGTTTTGGTTTTCCCTAATTTGGGGCTTTTTGCTTAATTTAGGGTTTTTTTGTTTTTGTTTTTGTTTGTTTTTAGGTTGATTCTAGTAAGAGGCCATTGTGTGATTATATACAGAAAGTTCAAAGGGATGTTAACGCGAGTATGAGGGGTGTTCTTGTGGATTGGTTGGTTGAAGTTGCTGAAGAATACAAGCTTGTTTCCGATACGCTTTATTTTTCGGTTTCTTATATTGATCGGTTTTTGTCGTTGAATGATTTAACTAGACAGAAGTTACAATTACTCGGTGTTGCTTCGATGTTGGTTGCTTCGTAAGTTTGTTTTTGTTTGTTGTTTTGATTTGTACTTTTGTATTGATGAATAAATACCTAATTTGGTCCCTCCCAATTTGTTTGCACCCAAATTAAAATAACACTCGCATTGGTTTGTTGCATTCTATAATGGTGGTTAAATTAGTTCCTCTATTAATTTTGACCCATTTTAACAGAGATTACTCTTATGAAAATGTCAGGAATTAATCTGGTATATTGGACTATAAACAAATTGTGAGGAGCCAAGATGTGATTTTATTGTTTATTTATTATTTATGCTTATTGATTTTTTATTTGTTTTGGATGTAGAAAATATGAAGAGATTAAGCCACCTGAGGTTGAGGACTTTTGTTACATTACGGACAATACATACTCCAAGGAAGAGGTGAATCTTGTAGTTTAAGTTTTTTGTTTGTGTTGTTGAATGTTTGTTTGGTTTTGGATCTTGCTGATTTTGGATGGTATTGTTTTGGTATTTTGTTTGATTAAAGGTTTTGACCATGGAGGCTGATATTTTGAAGTCATTGAAGTTTGAATTGGGTGGTCCTACAATAAAGACATTCCTAAGGCATGTGTGTTTTATTGATTATGTTTCTTTGTATGTTGAATGGAATTGTTTTTGAATGTTAGCTCACTTTTCATTTTTTCTTTTTCTGTTTGCAGAAGATTCATCACTAAAGTTGGTCTAGAGGGTGTTGATGTAAGTTATCTCATTCTTCAACCCATCTCATAGTAGTTATAGGTTATGACATGGATTCGGTGATTATGTGTGCTTATGTTCAACTTTTAGTAGTGTGGGACTTTTGGTTACTTTTGCTATTGTGTGTGCTTATGTCTTTATGACATTGATTATTCTCTCTTATCAAATAGTATCATCTAAGATAGGGAGATTGTTTGTGAAAATAAGAGATGTTGTATATAAATAGAAAGAGAATTAGAGTGGGTTTTGTCATGTCATTTGGAGAAATAGTTGAACTTTGAGCTTTTGGGAGGGTCTGAGATGGGAATTGAACCTTCTGGATAGTTCATTCAATACTAATTCATGAAAATCAAATTCCTATTTTGTTCTGCAGACTAACAAAACCCATGCAACCTTACTAACTTTTTTAAGAATACCTCTGCTGCATCTCTGGTGGCAAAGAGATGTTTCAACCCTAAATTTTTTGTCAAATGTGGCCAATCTAGCTAGTAGCCACTTTCACAAAGAAAGTATCTTTGGTTTGTGCTATAGGAAGTTTCAAATCTAGTTATCCAGAAGTTTGAAATCAATTTTACTTGGATTATTATTTATTACTCAAAATGTTGCAGGCAAACCCATTTCCGTTTATGTGTGCCAAATCTGGAGACTTTGGTTTGTTTAATACTTTTATCTCTTTTGTGCAGGCTTCTGAGTTGCAGTTTGAGTTCCTTTGCTCTTACCTTGCTGAGCTCAGTTTGTTGGATTATAATTGTGTGAAGTTCTTGCCTTCTATGGTGGCTGCATCAGTTGTATTTCTTGCTCGTTTTATGTTAAGCCCGAAGACTCATCCTTGGGTAATGTTTTAATTCGTGTACAATCTATATTTGGTATTTGACTTTGACACAGTTACTAAAATATTTCCATTTGATTACAGAATTCAGCCATCTATGAATTCACCAGATATAAACCTGCAGACTTGAAGGAATGTGTTCTAAATATACATGACTTGTACCTTGGTAGGAAAGGCGGCTCTTTGCAAGCTGTGAGAGATAAATACAAACAGCATAAGGTGATATTCCTGCACGCTTATTGTCAATCCTTTTTTACCAAACAAACTTAATTTGTCCTACCTTGGCCCAAGCTTTTTAAACAATTGAGTTATTTATTTCATTACACTTGTCACATCTGATGCATTTATGCTGATTCCATTTGTTGTTTTGGTCACAGTTCAAATGCGTGGCAACAACACCCTCTCCCCCGGAGATATCTCTTTCTTTTTTTGAATTTAGAGGGGCTGATCCTTAG

Protein sequence

>MS.gene00474.t1

MEPKSVNPTRRPQREAKKKAVAALCEQQRKRKRVPLGEITNDVITNANDVVLPTQKKRNVATSSNSDETPVPEKLEKFEDPQLCETYVSDIYDYLRNMEVDSSKRPLCDYIQKVQRDVNASMRGVLVDWLVEVAEEYKLVSDTLYFSVSYIDRFLSLNDLTRQKLQLLGVASMLVASKYEEIKPPEVEDFCYITDNTYSKEEVLTMEADILKSLKFELGGPTIKTFLRRFITKVGLEGVDASELQFEFLCSYLAELSLLDYNCVKFLPSMVAASVVFLARFMLSPKTHPWNSAIYEFTRYKPADLKECVLNIHDLYLGRKGGSLQAVRDKYKQHKFKCVATTPSPPEISLSFFEFRGADP