AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044085

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044085.t1 MTR_1g018680 94.262 366 12 3 1 360 1 363 0.0 674
MS.gene044085.t1 MTR_3g102520 55.621 338 134 5 30 354 23 357 8.48e-126 366
MS.gene044085.t1 MTR_3g102530 54.467 347 146 4 16 353 6 349 2.21e-122 357
MS.gene044085.t1 MTR_3g102510 53.681 326 140 5 30 354 23 338 8.65e-116 340
MS.gene044085.t1 MTR_3g102560 53.529 340 138 5 14 353 5 324 1.07e-114 336
MS.gene044085.t1 MTR_3g088415 53.237 278 125 3 78 354 231 504 6.96e-104 315
MS.gene044085.t1 MTR_3g102540 54.651 258 110 2 91 348 13 263 2.33e-96 288
MS.gene044085.t1 MTR_3g088405 49.281 278 136 3 79 354 235 509 4.01e-94 290
MS.gene044085.t1 MTR_3g107970 50.909 275 132 2 76 350 202 473 4.41e-92 284
MS.gene044085.t1 MTR_2g102520 50.741 270 128 3 78 345 206 472 1.09e-91 283
MS.gene044085.t1 MTR_1g011470 50.000 272 132 3 77 347 151 419 1.45e-88 273
MS.gene044085.t1 MTR_2g102550 46.909 275 143 2 71 345 151 422 6.38e-87 269
MS.gene044085.t1 MTR_3g088415 57.944 214 85 3 78 290 231 440 3.27e-84 262
MS.gene044085.t1 MTR_3g088415 59.064 171 66 2 78 248 231 397 5.69e-67 216
MS.gene044085.t1 MTR_7g089080 39.300 257 151 4 87 343 199 450 2.96e-53 189
MS.gene044085.t1 MTR_5g088980 38.211 246 141 5 87 331 165 400 1.92e-49 171
MS.gene044085.t1 MTR_8g074000 35.827 254 153 4 87 340 176 419 7.99e-49 170
MS.gene044085.t1 MTR_5g023790 36.015 261 157 4 80 340 165 415 9.46e-48 167
MS.gene044085.t1 MTR_5g023790 39.801 201 112 3 80 280 165 356 1.19e-43 155
MS.gene044085.t1 MTR_5g023790 42.105 152 85 2 80 231 165 313 3.57e-35 132
MS.gene044085.t1 MTR_3g060410 36.224 196 115 5 118 308 131 321 5.82e-25 104
MS.gene044085.t1 MTR_3g060770 33.937 221 129 6 118 333 56 264 9.08e-23 97.1
MS.gene044085.t1 MTR_3g061680 36.224 196 115 5 118 308 46 236 4.96e-22 94.7
MS.gene044085.t1 MTR_3g060820 35.714 196 116 5 118 308 46 236 5.17e-22 94.7
MS.gene044085.t1 MTR_3g060400 34.184 196 119 5 118 308 52 242 2.33e-21 92.8
MS.gene044085.t1 MTR_3g460990 34.184 196 119 5 118 308 52 242 2.33e-21 92.8
MS.gene044085.t1 MTR_5g044040 46.667 75 40 0 144 218 7 81 8.43e-19 82.0
MS.gene044085.t1 MTR_1g032850 30.366 191 120 6 123 309 372 553 3.95e-15 77.0
MS.gene044085.t1 MTR_1g107535 29.412 204 105 8 85 277 88 263 7.01e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044085.t1 AT5G43080 59.429 350 135 4 7 354 7 351 8.11e-146 416
MS.gene044085.t1 AT1G47230 57.895 361 134 6 7 354 6 361 3.10e-143 410
MS.gene044085.t1 AT5G43080 58.571 350 133 5 7 354 7 346 8.75e-142 406
MS.gene044085.t1 AT1G47230 57.735 362 134 7 7 354 6 362 1.48e-141 406
MS.gene044085.t1 AT1G47210 56.022 357 136 5 16 354 15 368 8.34e-137 394
MS.gene044085.t1 AT1G47220 56.376 298 121 2 57 354 34 322 4.82e-116 340
MS.gene044085.t1 AT1G44110 54.182 275 121 3 80 353 187 457 9.78e-102 308
MS.gene044085.t1 AT5G25380 50.360 278 127 3 79 352 165 435 2.10e-96 293
MS.gene044085.t1 AT5G25380 50.360 278 127 3 79 352 165 435 2.10e-96 293
MS.gene044085.t1 AT5G25380 50.360 278 127 3 79 352 171 441 4.22e-96 293
MS.gene044085.t1 AT5G11300 44.318 352 182 6 10 352 88 434 1.43e-94 289
MS.gene044085.t1 AT1G80370 52.672 262 121 2 79 340 189 447 4.84e-92 283
MS.gene044085.t1 AT5G25380 50.958 261 117 3 79 335 165 418 1.28e-91 281
MS.gene044085.t1 AT1G15570 51.685 267 126 2 79 345 180 443 1.96e-91 281
MS.gene044085.t1 AT1G77390 48.175 274 139 2 80 353 171 441 3.11e-88 273
MS.gene044085.t1 AT5G11300 44.407 295 150 6 10 295 88 377 1.48e-76 242
MS.gene044085.t1 AT3G11520 36.308 325 187 7 17 333 82 394 3.24e-51 176
MS.gene044085.t1 AT3G11520 38.047 297 164 7 17 305 82 366 7.07e-50 172
MS.gene044085.t1 AT5G06150 37.594 266 152 6 87 351 182 434 1.19e-48 170
MS.gene044085.t1 AT1G20610 34.211 266 164 5 80 345 170 424 7.40e-46 162
MS.gene044085.t1 AT1G47210 49.444 180 71 4 16 177 15 192 1.41e-45 154
MS.gene044085.t1 AT2G26760 37.594 266 155 5 87 351 130 385 1.51e-45 160
MS.gene044085.t1 AT4G37490 36.882 263 149 8 87 343 165 416 5.18e-44 157
MS.gene044085.t1 AT1G76310 35.060 251 152 5 87 336 177 417 3.11e-43 155
MS.gene044085.t1 AT2G17620 37.354 257 145 5 87 340 171 414 4.20e-42 152
MS.gene044085.t1 AT1G16330 33.457 269 166 5 66 331 371 629 9.89e-42 154
MS.gene044085.t1 AT4G35620 38.039 255 148 5 87 341 172 416 2.70e-41 150
MS.gene044085.t1 AT1G76310 37.879 198 113 4 87 283 177 365 1.33e-39 144
MS.gene044085.t1 AT1G76310 31.900 279 151 6 87 336 177 445 1.12e-37 140
MS.gene044085.t1 AT4G35620 40.580 207 113 5 87 293 172 368 1.10e-36 137
MS.gene044085.t1 AT5G06150 43.448 145 78 1 87 231 182 322 1.11e-35 133
MS.gene044085.t1 AT1G76310 41.781 146 81 3 87 231 177 319 1.76e-32 124
MS.gene044085.t1 AT1G20590 34.463 177 105 3 151 323 66 235 8.14e-25 102
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.16e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 269 389 2.28e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 269 389 2.28e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.29e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.29e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.29e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.29e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.32e-17 83.6
MS.gene044085.t1 AT1G34460 33.103 145 73 2 87 231 258 378 2.39e-17 83.6
MS.gene044085.t1 AT1G14750 28.736 261 166 9 65 319 163 409 1.13e-14 75.1
MS.gene044085.t1 AT1G14750 27.969 261 169 8 65 319 163 410 1.24e-14 75.1
MS.gene044085.t1 AT1G14750 30.348 201 129 6 122 319 386 578 1.48e-14 75.5
MS.gene044085.t1 AT1G14750 30.366 191 123 5 122 309 386 569 6.35e-14 73.6
MS.gene044085.t1 AT1G70210 30.726 179 111 5 106 276 64 237 7.74e-11 63.2

Find 70 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAAGTTCTTGCCTTCTA+TGG 0.257368 1.2:-4713430 MS.gene044085:CDS
CGCGTGGGGTTAACGCTCTT+TGG 0.262421 1.2:+4715519 None:intergenic
CTTAGGAATGTCTTTATTGT+AGG 0.290255 1.2:+4714334 None:intergenic
CCTTGCTGAGCTCAGTTTGT+TGG 0.292382 1.2:-4713463 MS.gene044085:CDS
AACTCCGGTACCGGAGAAAT+TGG 0.300734 1.2:-4715322 MS.gene044085:CDS
CAAGTCTGCAGGTTTATATC+TGG 0.333404 1.2:+4713246 None:intergenic
GAAGTCATTGAAGTTTGAAT+TGG 0.360491 1.2:-4714362 MS.gene044085:CDS
GGGATGTTAACGCGAGTATG+AGG 0.393481 1.2:-4715070 MS.gene044085:CDS
ATATAAACCTGCAGACTTGA+AGG 0.399847 1.2:-4713242 MS.gene044085:CDS
GGGTGTTCTTGTGGATTGGT+TGG 0.400845 1.2:-4715048 MS.gene044085:CDS
ACTGATGCAGCCACCATAGA+AGG 0.402548 1.2:+4713417 None:intergenic
TTCTCCAATTTCTCCGGTAC+CGG 0.411453 1.2:+4715318 None:intergenic
ATATCTGGTGAATTCATAGA+TGG 0.420121 1.2:+4713261 None:intergenic
TTACCCAAGGATGAGTCTTC+GGG 0.425884 1.2:+4713367 None:intergenic
ATTACCCAAGGATGAGTCTT+CGG 0.430153 1.2:+4713366 None:intergenic
TTTAGGTTGATTCTAGTAAG+AGG 0.436695 1.2:-4715124 MS.gene044085:intron
TCACGTTGAGGGCGGCGCGT+GGG 0.441478 1.2:+4715504 None:intergenic
TACAAGATTCACCTCTTCCT+TGG 0.443619 1.2:+4714505 None:intergenic
CTCCGGTACCGGAGTTTCAT+CGG 0.445846 1.2:+4715329 None:intergenic
TCGAATTTCTCCAATTTCTC+CGG 0.446675 1.2:+4715312 None:intergenic
TGAGGGGTGTTCTTGTGGAT+TGG 0.457804 1.2:-4715052 MS.gene044085:CDS
AGAAGATTCATCACTAAAGT+TGG 0.459150 1.2:-4714218 MS.gene044085:CDS
ACACATTCCTTCAAGTCTGC+AGG 0.460260 1.2:+4713235 None:intergenic
CTCCGATGAAACTCCGGTAC+CGG 0.462507 1.2:-4715331 MS.gene044085:CDS
TTTCGGTTTCTTATATTGAT+CGG 0.465083 1.2:-4714980 MS.gene044085:CDS
AAGTCATTGAAGTTTGAATT+GGG 0.468127 1.2:-4714361 MS.gene044085:CDS
CACGTTGAGGGCGGCGCGTG+GGG 0.493799 1.2:+4715505 None:intergenic
GATTAAGCCACCTGAGGTTG+AGG 0.495688 1.2:-4714558 MS.gene044085:CDS
AGATAAATACAAACAGCATA+AGG 0.498310 1.2:-4713155 MS.gene044085:intron
GGCAACAACACCCTCTCCCC+CGG 0.503387 1.2:-4712964 MS.gene044085:CDS
AATATACATGACTTGTACCT+TGG 0.507114 1.2:-4713208 MS.gene044085:CDS
TTGTTCACATAGTGCAGCTA+CGG 0.511501 1.2:+4715467 None:intergenic
TTCACGTTGAGGGCGGCGCG+TGG 0.513771 1.2:+4715503 None:intergenic
TTGGTCACAGTTCAAATGCA+TGG 0.517807 1.2:-4712985 MS.gene044085:intron
CTTCAAAATATCAGCCTCCA+TGG 0.525850 1.2:+4714381 None:intergenic
GAGTATGAGGGGTGTTCTTG+TGG 0.531293 1.2:-4715057 MS.gene044085:CDS
ATCACTAAAGTTGGTCTAGA+GGG 0.532834 1.2:-4714209 MS.gene044085:CDS
AAGCCCGAAGACTCATCCTT+GGG 0.535304 1.2:-4713370 MS.gene044085:intron
TAACAAAAGTCCTCAACCTC+AGG 0.540151 1.2:+4714548 None:intergenic
GAAGTTCTTGCCTTCTATGG+TGG 0.548750 1.2:-4713427 MS.gene044085:CDS
TAAGCCCGAAGACTCATCCT+TGG 0.550073 1.2:-4713371 MS.gene044085:CDS
GGATGTTAACGCGAGTATGA+GGG 0.553144 1.2:-4715069 MS.gene044085:CDS
ACTCGGTGTTGCTTCGATGT+TGG 0.553532 1.2:-4714916 MS.gene044085:CDS
ATCAAACTCCGATGAAACTC+CGG 0.553690 1.2:-4715337 MS.gene044085:CDS
ATTATATACAGAAAGTTCAA+AGG 0.558828 1.2:-4715091 MS.gene044085:CDS
TTATATACAGAAAGTTCAAA+GGG 0.568073 1.2:-4715090 MS.gene044085:CDS
TTACGATTATCTTCGTAACA+TGG 0.581267 1.2:-4715253 MS.gene044085:CDS
GACTTGTACCTTGGTAGGAA+AGG 0.582896 1.2:-4713199 MS.gene044085:CDS
AGACAGAAGTTGCAATTACT+CGG 0.583486 1.2:-4714933 MS.gene044085:CDS
CAATACATACTCCAAGGAAG+AGG 0.589667 1.2:-4714516 MS.gene044085:intron
AAACGAAAACGTGTTCCTCT+CGG 0.594016 1.2:-4715438 MS.gene044085:CDS
CATCACTAAAGTTGGTCTAG+AGG 0.599941 1.2:-4714210 MS.gene044085:CDS
GCTCAGCAAGGTAAGAGCAA+AGG 0.603798 1.2:+4713475 None:intergenic
TACATGACTTGTACCTTGGT+AGG 0.612523 1.2:-4713204 MS.gene044085:CDS
CTACAATAAAGACATTCCTA+AGG 0.613211 1.2:-4714333 MS.gene044085:intron
CAAAAGTCCTCAACCTCAGG+TGG 0.618973 1.2:+4714551 None:intergenic
TCATTGAAGTTTGAATTGGG+TGG 0.631240 1.2:-4714358 MS.gene044085:CDS
GATGTTAACGCGAGTATGAG+GGG 0.631509 1.2:-4715068 MS.gene044085:CDS
TCTTTGCTTCACGTTGAGGG+CGG 0.632157 1.2:+4715496 None:intergenic
TACGGACAATACATACTCCA+AGG 0.633308 1.2:-4714522 MS.gene044085:CDS
TTGTACCTTGGTAGGAAAGG+CGG 0.635313 1.2:-4713196 MS.gene044085:CDS
CGATTATCTTCGTAACATGG+AGG 0.645441 1.2:-4715250 MS.gene044085:intron
AAGAGCCGCCTTTCCTACCA+AGG 0.647189 1.2:+4713191 None:intergenic
CCAACAAACTGAGCTCAGCA+AGG 0.668066 1.2:+4713463 None:intergenic
TCGTTTGTTATTTCACCGAG+AGG 0.670866 1.2:+4715423 None:intergenic
TTCTGTATATAATCACACAA+TGG 0.674639 1.2:+4715101 None:intergenic
AAAGAAAGAGATATCTCCGG+GGG 0.686776 1.2:+4712948 None:intergenic
TGAAGAGATTAAGCCACCTG+AGG 0.697941 1.2:-4714564 MS.gene044085:CDS
ACATAAGTTTCACATAACTG+AGG 0.720028 1.2:+4715285 None:intergenic
AAAAGAAAGAGATATCTCCG+GGG 0.745816 1.2:+4712947 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGATTTTTTATTTGTTT+TGG - chr1.2:4713846-4713865 MS.gene044085:intron 10.0%
!! TATTCTTAAAAAAGTTAGTA+AGG + chr1.2:4714661-4714680 None:intergenic 15.0%
!! TTAACTTCAAATTTCAATTA+GGG - chr1.2:4713236-4713255 MS.gene044085:CDS 15.0%
!!! TTTAACTTCAAATTTCAATT+AGG - chr1.2:4713235-4713254 MS.gene044085:CDS 15.0%
!!! TTTCTTTTTTTGAATTTAGA+GGG - chr1.2:4715513-4715532 MS.gene044085:CDS 15.0%
!!! TTTTGTTTTTGTTTGTTTTT+AGG - chr1.2:4713304-4713323 MS.gene044085:intron 15.0%
!! AATTATAATAACACTCACAT+TGG - chr1.2:4713647-4713666 MS.gene044085:intron 20.0%
!! AGTAATCTCTTTGTTAAAAT+GGG + chr1.2:4713723-4713742 None:intergenic 20.0%
!! ATTATATACAGAAAGTTCAA+AGG - chr1.2:4713354-4713373 MS.gene044085:intron 20.0%
!! CACATTTGACAAAAAATTTA+GGG + chr1.2:4714721-4714740 None:intergenic 20.0%
!! TGATGAATAAATACCTAATT+TGG - chr1.2:4713602-4713621 MS.gene044085:intron 20.0%
!! TTATATACAGAAAGTTCAAA+GGG - chr1.2:4713355-4713374 MS.gene044085:intron 20.0%
!!! AAATAGTATCATCTAAGATA+GGG - chr1.2:4714430-4714449 MS.gene044085:intron 20.0%
!!! AGTTTGAAATCAATTTTACT+TGG - chr1.2:4714822-4714841 MS.gene044085:intron 20.0%
!!! ATGTGAGTGTTATTATAATT+TGG + chr1.2:4713647-4713666 None:intergenic 20.0%
!!! CTTTCTTTTTTTGAATTTAG+AGG - chr1.2:4715512-4715531 MS.gene044085:CDS 20.0%
!!! TAAAATTGATTTCAAACTTC+TGG + chr1.2:4714820-4714839 None:intergenic 20.0%
!!! TGTGAGTGTTATTATAATTT+GGG + chr1.2:4713646-4713665 None:intergenic 20.0%
!!! TTAAGTTTGTTTGGTAAAAA+AGG + chr1.2:4715343-4715362 None:intergenic 20.0%
!!! TTCTTTTTTTGAATTTAGAG+GGG - chr1.2:4715514-4715533 MS.gene044085:CDS 20.0%
!!! TTGGTATTTTGTTTGATTAA+AGG - chr1.2:4714035-4714054 MS.gene044085:intron 20.0%
! AAAAAAGAAAGAGATATCTC+CGG + chr1.2:4715503-4715522 None:intergenic 25.0%
! AAATAGAAAGAGAATTAGAG+TGG - chr1.2:4714486-4714505 MS.gene044085:intron 25.0%
! AAATGGGTCAAAATTAATAG+AGG + chr1.2:4713707-4713726 None:intergenic 25.0%
! AAGTCATTGAAGTTTGAATT+GGG - chr1.2:4714084-4714103 MS.gene044085:intron 25.0%
! AATAGAAAGAGAATTAGAGT+GGG - chr1.2:4714487-4714506 MS.gene044085:intron 25.0%
! ACTCAATTGTTTAAAAAGCT+TGG + chr1.2:4715382-4715401 None:intergenic 25.0%
! AGATAAATACAAACAGCATA+AGG - chr1.2:4715290-4715309 MS.gene044085:CDS 25.0%
! ATGTTTCTTTGTATGTTGAA+TGG - chr1.2:4714154-4714173 MS.gene044085:intron 25.0%
! ATTACTCTTATGAAAATGTC+AGG - chr1.2:4713737-4713756 MS.gene044085:intron 25.0%
! ATTCGTGTACAATCTATATT+TGG - chr1.2:4715108-4715127 MS.gene044085:CDS 25.0%
! CCACATTTGACAAAAAATTT+AGG + chr1.2:4714722-4714741 None:intergenic 25.0%
! CTCAATTGTTTAAAAAGCTT+GGG + chr1.2:4715381-4715400 None:intergenic 25.0%
! GAGTAATCTCTTTGTTAAAA+TGG + chr1.2:4713724-4713743 None:intergenic 25.0%
! TATAGACTAAAATCACATCT+TGG + chr1.2:4713804-4713823 None:intergenic 25.0%
! TTCTGTATATAATCACACAA+TGG + chr1.2:4713347-4713366 None:intergenic 25.0%
! TTTATTACTCAAAATGTTGC+AGG - chr1.2:4714852-4714871 MS.gene044085:intron 25.0%
! TTTCGGTTTCTTATATTGAT+CGG - chr1.2:4713465-4713484 MS.gene044085:CDS 25.0%
!! CAAATAGTATCATCTAAGAT+AGG - chr1.2:4714429-4714448 MS.gene044085:intron 25.0%
!! TAGGACAAATTAAGTTTGTT+TGG + chr1.2:4715352-4715371 None:intergenic 25.0%
!! TCAAATTTCAATTAGGGTTT+TGG - chr1.2:4713242-4713261 MS.gene044085:CDS 25.0%
!!! CCTAAATTTTTTGTCAAATG+TGG - chr1.2:4714719-4714738 MS.gene044085:intron 25.0%
!!! TTTCACAAAGAAAGTATCTT+TGG - chr1.2:4714762-4714781 MS.gene044085:intron 25.0%
AAAAAGAAAGAGATATCTCC+GGG + chr1.2:4715502-4715521 None:intergenic 30.0%
AAAAAGTTAGTAAGGTTGCA+TGG + chr1.2:4714653-4714672 None:intergenic 30.0%
AAAAGTTAGTAAGGTTGCAT+GGG + chr1.2:4714652-4714671 None:intergenic 30.0%
AACCGAAAAATAAAGCGTAT+CGG + chr1.2:4713453-4713472 None:intergenic 30.0%
AACCTATAACTACTATGAGA+TGG + chr1.2:4714291-4714310 None:intergenic 30.0%
AATTTGGGTGCAAACAAATT+GGG + chr1.2:4713631-4713650 None:intergenic 30.0%
ACATAAGTTTCACATAACTG+AGG + chr1.2:4713163-4713182 None:intergenic 30.0%
ACCTATAACTACTATGAGAT+GGG + chr1.2:4714290-4714309 None:intergenic 30.0%
ACGAATTAAAACATTACCCA+AGG + chr1.2:4715094-4715113 None:intergenic 30.0%
AGAAGATTCATCACTAAAGT+TGG - chr1.2:4714227-4714246 MS.gene044085:intron 30.0%
AGATACTTTCTTTGTGAAAG+TGG + chr1.2:4714761-4714780 None:intergenic 30.0%
AGTAGTTATAGGTTATGACA+TGG - chr1.2:4714297-4714316 MS.gene044085:intron 30.0%
ATATCTGGTGAATTCATAGA+TGG + chr1.2:4715187-4715206 None:intergenic 30.0%
CAGGAATTAATCTGGTATAT+TGG - chr1.2:4713756-4713775 MS.gene044085:intron 30.0%
CTACAATAAAGACATTCCTA+AGG - chr1.2:4714112-4714131 MS.gene044085:intron 30.0%
CTTAGGAATGTCTTTATTGT+AGG + chr1.2:4714114-4714133 None:intergenic 30.0%
GAAAATGTCAGGAATTAATC+TGG - chr1.2:4713748-4713767 MS.gene044085:intron 30.0%
GAAGTCATTGAAGTTTGAAT+TGG - chr1.2:4714083-4714102 MS.gene044085:intron 30.0%
TAATTTGGGTGCAAACAAAT+TGG + chr1.2:4713632-4713651 None:intergenic 30.0%
TATAGGTTATGACATGGATT+CGG - chr1.2:4714303-4714322 MS.gene044085:intron 30.0%
TATTGAATGAACTATCCAGA+AGG + chr1.2:4714581-4714600 None:intergenic 30.0%
TGGACTATAAACAAATTGTG+AGG - chr1.2:4713776-4713795 MS.gene044085:intron 30.0%
TTACGATTATCTTCGTAACA+TGG - chr1.2:4713192-4713211 MS.gene044085:CDS 30.0%
TTTGTTGCATTCTATAATGG+TGG - chr1.2:4713670-4713689 MS.gene044085:intron 30.0%
! AATATACATGACTTGTACCT+TGG - chr1.2:4715237-4715256 MS.gene044085:intron 30.0%
! ATAGTTGAACTTTGAGCTTT+TGG - chr1.2:4714532-4714551 MS.gene044085:CDS 30.0%
! ATGTTCAACTTTTAGTAGTG+TGG - chr1.2:4714341-4714360 MS.gene044085:CDS 30.0%
! GATTTTGGATGGTATTGTTT+TGG - chr1.2:4714016-4714035 MS.gene044085:intron 30.0%
! TACTTTTATCTCTTTTGTGC+AGG - chr1.2:4714925-4714944 MS.gene044085:CDS 30.0%
! TAGTTGAACTTTGAGCTTTT+GGG - chr1.2:4714533-4714552 MS.gene044085:CDS 30.0%
! TGAGGACTTTTGTTACATTA+CGG - chr1.2:4713905-4713924 MS.gene044085:intron 30.0%
! TGTGTTGTTGAATGTTTGTT+TGG - chr1.2:4713979-4713998 MS.gene044085:intron 30.0%
! TGTTCAACTTTTAGTAGTGT+GGG - chr1.2:4714342-4714361 MS.gene044085:CDS 30.0%
!! AAACTTAATTTGTCCTACCT+TGG - chr1.2:4715355-4715374 MS.gene044085:CDS 30.0%
!! GTTGAATGTTTGTTTGGTTT+TGG - chr1.2:4713985-4714004 MS.gene044085:intron 30.0%
!! TGGTTTGTTGCATTCTATAA+TGG - chr1.2:4713667-4713686 MS.gene044085:intron 30.0%
!! TTGATTAAAGGTTTTGACCA+TGG - chr1.2:4714047-4714066 MS.gene044085:intron 30.0%
!! TTTAGGTTGATTCTAGTAAG+AGG - chr1.2:4713321-4713340 MS.gene044085:intron 30.0%
!!! GTTTTGGTTTTCCCTAATTT+GGG - chr1.2:4713258-4713277 MS.gene044085:CDS 30.0%
!!! TTCCGATACGCTTTATTTTT+CGG - chr1.2:4713448-4713467 MS.gene044085:CDS 30.0%
!!! TTTTGGTTTTCCCTAATTTG+GGG - chr1.2:4713259-4713278 MS.gene044085:CDS 30.0%
AAAAGAAAGAGATATCTCCG+GGG + chr1.2:4715501-4715520 None:intergenic 35.0%
AAGCAAAAAGCCCCAAATTA+GGG + chr1.2:4713272-4713291 None:intergenic 35.0%
ACAAATTGGGAGACCAAATT+AGG + chr1.2:4713618-4713637 None:intergenic 35.0%
ACCCATCTCATAGTAGTTAT+AGG - chr1.2:4714286-4714305 MS.gene044085:intron 35.0%
ACTGTGACCAAAACAACAAA+TGG + chr1.2:4715451-4715470 None:intergenic 35.0%
AGACAGAAGTTGCAATTACT+CGG - chr1.2:4713512-4713531 MS.gene044085:intron 35.0%
ATATAAACCTGCAGACTTGA+AGG - chr1.2:4715203-4715222 MS.gene044085:intron 35.0%
CAATAAAACACACATGCCTT+AGG + chr1.2:4714131-4714150 None:intergenic 35.0%
GGCTGAATTCTGTAATCAAA+TGG + chr1.2:4715166-4715185 None:intergenic 35.0%
TAAGCAAAAAGCCCCAAATT+AGG + chr1.2:4713273-4713292 None:intergenic 35.0%
TCATTGAAGTTTGAATTGGG+TGG - chr1.2:4714087-4714106 MS.gene044085:intron 35.0%
TCGAATTTCTCCAATTTCTC+CGG + chr1.2:4713136-4713155 None:intergenic 35.0%
! ATCACTAAAGTTGGTCTAGA+GGG - chr1.2:4714236-4714255 MS.gene044085:intron 35.0%
! GGGGCTTTTTGCTTAATTTA+GGG - chr1.2:4713278-4713297 MS.gene044085:intron 35.0%
! TGGGGCTTTTTGCTTAATTT+AGG - chr1.2:4713277-4713296 MS.gene044085:intron 35.0%
! TTTTAGTAGTGTGGGACTTT+TGG - chr1.2:4714350-4714369 MS.gene044085:CDS 35.0%
! TTTTCTTTGCTTCACGTTGA+GGG + chr1.2:4712955-4712974 None:intergenic 35.0%
! TTTTTCTTTGCTTCACGTTG+AGG + chr1.2:4712956-4712975 None:intergenic 35.0%
!! ATTAAAGGTTTTGACCATGG+AGG - chr1.2:4714050-4714069 MS.gene044085:intron 35.0%
!! GTATCTTTGGTTTGTGCTAT+AGG - chr1.2:4714775-4714794 MS.gene044085:intron 35.0%
!!! GCTGATTCCATTTGTTGTTT+TGG - chr1.2:4715441-4715460 MS.gene044085:CDS 35.0%
!!! GGTTTTGGTTTTCCCTAATT+TGG - chr1.2:4713257-4713276 MS.gene044085:CDS 35.0%
!!! GTTTTGGATCTTGCTGATTT+TGG - chr1.2:4714001-4714020 MS.gene044085:intron 35.0%
AAACGAAAACGTGTTCCTCT+CGG - chr1.2:4713007-4713026 MS.gene044085:intron 40.0%
AAAGAAAGAGATATCTCCGG+GGG + chr1.2:4715500-4715519 None:intergenic 40.0%
AAAGTGGCTACTAGCTAGAT+TGG + chr1.2:4714745-4714764 None:intergenic 40.0%
ATCAAACTCCGATGAAACTC+CGG - chr1.2:4713108-4713127 MS.gene044085:intron 40.0%
ATTACCCAAGGATGAGTCTT+CGG + chr1.2:4715082-4715101 None:intergenic 40.0%
ATTTGGCACACAAACGGAAA+TGG + chr1.2:4714883-4714902 None:intergenic 40.0%
CAAACCAAAGTCTCCAGATT+TGG + chr1.2:4714900-4714919 None:intergenic 40.0%
CAAGTCTGCAGGTTTATATC+TGG + chr1.2:4715202-4715221 None:intergenic 40.0%
CAATACATACTCCAAGGAAG+AGG - chr1.2:4713929-4713948 MS.gene044085:intron 40.0%
CGATTATCTTCGTAACATGG+AGG - chr1.2:4713195-4713214 MS.gene044085:CDS 40.0%
CTTCAAAATATCAGCCTCCA+TGG + chr1.2:4714067-4714086 None:intergenic 40.0%
TAACAAAAGTCCTCAACCTC+AGG + chr1.2:4713900-4713919 None:intergenic 40.0%
TACAAGATTCACCTCTTCCT+TGG + chr1.2:4713943-4713962 None:intergenic 40.0%
TACGGACAATACATACTCCA+AGG - chr1.2:4713923-4713942 MS.gene044085:intron 40.0%
TGTGAAGTTCTTGCCTTCTA+TGG - chr1.2:4715015-4715034 MS.gene044085:CDS 40.0%
TGTTTAAAAAGCTTGGGCCA+AGG + chr1.2:4715375-4715394 None:intergenic 40.0%
TTGGTCACAGTTCAAATGCA+TGG - chr1.2:4715460-4715479 MS.gene044085:CDS 40.0%
TTGTTCACATAGTGCAGCTA+CGG + chr1.2:4712981-4713000 None:intergenic 40.0%
TTTGGCACACAAACGGAAAT+GGG + chr1.2:4714882-4714901 None:intergenic 40.0%
! CATCACTAAAGTTGGTCTAG+AGG - chr1.2:4714235-4714254 MS.gene044085:intron 40.0%
! GTTAGTCTGCAGAACAAGAT+AGG + chr1.2:4714625-4714644 None:intergenic 40.0%
! TACATGACTTGTACCTTGGT+AGG - chr1.2:4715241-4715260 MS.gene044085:intron 40.0%
! TTGAACTTTGAGCTTTTGGG+AGG - chr1.2:4714536-4714555 MS.gene044085:CDS 40.0%
!! GTGGGTTTTGTCATGTCATT+TGG - chr1.2:4714505-4714524 MS.gene044085:intron 40.0%
!! TCGTTTGTTATTTCACCGAG+AGG + chr1.2:4713025-4713044 None:intergenic 40.0%
!! TGAACTTTGAGCTTTTGGGA+GGG - chr1.2:4714537-4714556 MS.gene044085:CDS 40.0%
!!! ACGTTTCGTTTTTTCTGCGT+TGG + chr1.2:4713079-4713098 None:intergenic 40.0%
!!! TGGATCTTGCTGATTTTGGA+TGG - chr1.2:4714005-4714024 MS.gene044085:intron 40.0%
ACACATTCCTTCAAGTCTGC+AGG + chr1.2:4715213-4715232 None:intergenic 45.0%
AGGATTGACAATAAGCGTGC+AGG + chr1.2:4715323-4715342 None:intergenic 45.0%
CTCCAGATTTGGCACACAAA+CGG + chr1.2:4714889-4714908 None:intergenic 45.0%
GAAGTTCTTGCCTTCTATGG+TGG - chr1.2:4715018-4715037 MS.gene044085:CDS 45.0%
GACTTGTACCTTGGTAGGAA+AGG - chr1.2:4715246-4715265 MS.gene044085:intron 45.0%
GAGATGGGAATTGAACCTTC+TGG - chr1.2:4714563-4714582 MS.gene044085:CDS 45.0%
GATGTTAACGCGAGTATGAG+GGG - chr1.2:4713377-4713396 MS.gene044085:CDS 45.0%
GGATGTTAACGCGAGTATGA+GGG - chr1.2:4713376-4713395 MS.gene044085:CDS 45.0%
TAAAAAGCTTGGGCCAAGGT+AGG + chr1.2:4715371-4715390 None:intergenic 45.0%
TGAAGAGATTAAGCCACCTG+AGG - chr1.2:4713881-4713900 MS.gene044085:intron 45.0%
TGTGCCAAATCTGGAGACTT+TGG - chr1.2:4714893-4714912 MS.gene044085:intron 45.0%
TTACCCAAGGATGAGTCTTC+GGG + chr1.2:4715081-4715100 None:intergenic 45.0%
TTCCGTTTGTGTGCCAAATC+TGG - chr1.2:4714884-4714903 MS.gene044085:intron 45.0%
TTCTCCAATTTCTCCGGTAC+CGG + chr1.2:4713130-4713149 None:intergenic 45.0%
TTGTACCTTGGTAGGAAAGG+CGG - chr1.2:4715249-4715268 MS.gene044085:intron 45.0%
AAGCCCGAAGACTCATCCTT+GGG - chr1.2:4715075-4715094 MS.gene044085:CDS 50.0%
ACTGATGCAGCCACCATAGA+AGG + chr1.2:4715031-4715050 None:intergenic 50.0%
CAAAAGTCCTCAACCTCAGG+TGG + chr1.2:4713897-4713916 None:intergenic 50.0%
CCAACAAACTGAGCTCAGCA+AGG + chr1.2:4714985-4715004 None:intergenic 50.0%
CCTTGCTGAGCTCAGTTTGT+TGG - chr1.2:4714982-4715001 MS.gene044085:CDS 50.0%
GAATACCTCTGCTGCATCTC+TGG - chr1.2:4714676-4714695 MS.gene044085:intron 50.0%
GATTAAGCCACCTGAGGTTG+AGG - chr1.2:4713887-4713906 MS.gene044085:intron 50.0%
GCTCAGCAAGGTAAGAGCAA+AGG + chr1.2:4714973-4714992 None:intergenic 50.0%
GGGATGTTAACGCGAGTATG+AGG - chr1.2:4713375-4713394 MS.gene044085:CDS 50.0%
TAAGCCCGAAGACTCATCCT+TGG - chr1.2:4715074-4715093 MS.gene044085:CDS 50.0%
TCTTTGCTTCACGTTGAGGG+CGG + chr1.2:4712952-4712971 None:intergenic 50.0%
! AACTCCGGTACCGGAGAAAT+TGG - chr1.2:4713123-4713142 MS.gene044085:intron 50.0%
!! ACTCGGTGTTGCTTCGATGT+TGG - chr1.2:4713529-4713548 MS.gene044085:intron 50.0%
!! CTTTTGGGAGGGTCTGAGAT+GGG - chr1.2:4714548-4714567 MS.gene044085:CDS 50.0%
!! GAGTATGAGGGGTGTTCTTG+TGG - chr1.2:4713388-4713407 MS.gene044085:CDS 50.0%
!! GGGTGTTCTTGTGGATTGGT+TGG - chr1.2:4713397-4713416 MS.gene044085:CDS 50.0%
!! TGAGGGGTGTTCTTGTGGAT+TGG - chr1.2:4713393-4713412 MS.gene044085:CDS 50.0%
AAGAGATATCTCCGGGGGAG+AGG + chr1.2:4715495-4715514 None:intergenic 55.0%
AAGAGCCGCCTTTCCTACCA+AGG + chr1.2:4715257-4715276 None:intergenic 55.0%
AGAGATATCTCCGGGGGAGA+GGG + chr1.2:4715494-4715513 None:intergenic 55.0%
CTCCGATGAAACTCCGGTAC+CGG - chr1.2:4713114-4713133 MS.gene044085:intron 55.0%
TACCTCTGCTGCATCTCTGG+TGG - chr1.2:4714679-4714698 MS.gene044085:intron 55.0%
! CTCCGGTACCGGAGTTTCAT+CGG + chr1.2:4713119-4713138 None:intergenic 55.0%
!! GCTTTTGGGAGGGTCTGAGA+TGG - chr1.2:4714547-4714566 MS.gene044085:CDS 55.0%
CGCGTGGGGTTAACGCTCTT+TGG + chr1.2:4712929-4712948 None:intergenic 60.0%
TGCCACCAGAGATGCAGCAG+AGG + chr1.2:4714684-4714703 None:intergenic 60.0%
GGCAACAACACCCTCTCCCC+CGG - chr1.2:4715481-4715500 MS.gene044085:CDS 65.0%
TCACGTTGAGGGCGGCGCGT+GGG + chr1.2:4712944-4712963 None:intergenic 70.0%
TTCACGTTGAGGGCGGCGCG+TGG + chr1.2:4712945-4712964 None:intergenic 70.0%
CACGTTGAGGGCGGCGCGTG+GGG + chr1.2:4712943-4712962 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 4712920 4715547 4712920 ID=MS.gene044085
chr1.2 mRNA 4712920 4715547 4712920 ID=MS.gene044085.t1;Parent=MS.gene044085
chr1.2 exon 4715251 4715547 4715251 ID=MS.gene044085.t1.exon1;Parent=MS.gene044085.t1
chr1.2 CDS 4715251 4715547 4715251 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4714909 4715141 4714909 ID=MS.gene044085.t1.exon2;Parent=MS.gene044085.t1
chr1.2 CDS 4714909 4715141 4714909 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4714517 4714592 4714517 ID=MS.gene044085.t1.exon3;Parent=MS.gene044085.t1
chr1.2 CDS 4714517 4714592 4714517 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4714334 4714410 4714334 ID=MS.gene044085.t1.exon4;Parent=MS.gene044085.t1
chr1.2 CDS 4714334 4714410 4714334 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4714202 4714238 4714202 ID=MS.gene044085.t1.exon5;Parent=MS.gene044085.t1
chr1.2 CDS 4714202 4714238 4714202 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4713371 4713520 4713371 ID=MS.gene044085.t1.exon6;Parent=MS.gene044085.t1
chr1.2 CDS 4713371 4713520 4713371 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4713156 4713290 4713156 ID=MS.gene044085.t1.exon7;Parent=MS.gene044085.t1
chr1.2 CDS 4713156 4713290 4713156 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
chr1.2 exon 4712920 4712997 4712920 ID=MS.gene044085.t1.exon8;Parent=MS.gene044085.t1
chr1.2 CDS 4712920 4712997 4712920 ID=cds.MS.gene044085.t1;Parent=MS.gene044085.t1
Gene Sequence

>MS.gene044085

ATGGAACCAAAGAGCGTTAACCCCACGCGCCGCCCTCAACGTGAAGCAAAGAAAAAAGCCGTAGCTGCACTATGTGAACAACAACGCAAACGAAAACGTGTTCCTCTCGGTGAAATAACAAACGACGTAATCACAAACGCAAACGATGTCGTTTTACCAACGCAGAAAAAACGAAACGTTGCTACTTCATCAAACTCCGATGAAACTCCGGTACCGGAGAAATTGGAGAAATTCGAAGATCCTCAGTTATGTGAAACTTATGTTTCTGATATTTACGATTATCTTCGTAACATGGAGGTAATTTGATTTACTTAATTTAACTTCAAATTTCAATTAGGGTTTTGGTTTTCCCTAATTTGGGGCTTTTTGCTTAATTTAGGGTTTTTTTGTTTTTGTTTGTTTTTAGGTTGATTCTAGTAAGAGGCCATTGTGTGATTATATACAGAAAGTTCAAAGGGATGTTAACGCGAGTATGAGGGGTGTTCTTGTGGATTGGTTGGTTGAAGTTGCTGAAGAATACAAGCTTGTTTCCGATACGCTTTATTTTTCGGTTTCTTATATTGATCGGTTTTTGTCGTTGAATGATTTAACTAGACAGAAGTTGCAATTACTCGGTGTTGCTTCGATGTTGGTTGCTTCGTAAGTTTGTTTTTGTTTGTTGTTTTGATTTGTACTTTTGTATTGATGAATAAATACCTAATTTGGTCTCCCAATTTGTTTGCACCCAAATTATAATAACACTCACATTGGTTTGTTGCATTCTATAATGGTGGTTAAATTAGTTCCTCTATTAATTTTGACCCATTTTAACAAAGAGATTACTCTTATGAAAATGTCAGGAATTAATCTGGTATATTGGACTATAAACAAATTGTGAGGAGCCAAGATGTGATTTTAGTCTATATTGTTTATTTATTATTTATGCTTATTGATTTTTTATTTGTTTTGGATGTAGAAAATATGAAGAGATTAAGCCACCTGAGGTTGAGGACTTTTGTTACATTACGGACAATACATACTCCAAGGAAGAGGTGAATCTTGTAGTTTTAGTTTTTTGTTTGTGTTGTTGAATGTTTGTTTGGTTTTGGATCTTGCTGATTTTGGATGGTATTGTTTTGGTATTTTGTTTGATTAAAGGTTTTGACCATGGAGGCTGATATTTTGAAGTCATTGAAGTTTGAATTGGGTGGTCCTACAATAAAGACATTCCTAAGGCATGTGTGTTTTATTGATTATGTTTCTTTGTATGTTGAATGGAATTGTTTTTGAATGTTAGCTCACTTTTCATTTTTTCTTTTTCTGTTTGCAGAAGATTCATCACTAAAGTTGGTCTAGAGGGTGTTGATGTAAGTTATCTCATTCTTCAACCCATCTCATAGTAGTTATAGGTTATGACATGGATTCGGTGATTATGTGTGCTTATGTTCAACTTTTAGTAGTGTGGGACTTTTGGTTACTTTTGCTATTGTGTGTGCTTATGTCTTTATGACATTGATTATTCTCTCTTATCAAATAGTATCATCTAAGATAGGGAGATTGTTTGTGAAAATAAGAGATGTTGTATATAAATAGAAAGAGAATTAGAGTGGGTTTTGTCATGTCATTTGGAGAAATAGTTGAACTTTGAGCTTTTGGGAGGGTCTGAGATGGGAATTGAACCTTCTGGATAGTTCATTCAATACTAATTCATGAAAATCAAATTCCTATCTTGTTCTGCAGACTAACAAAACCCATGCAACCTTACTAACTTTTTTAAGAATACCTCTGCTGCATCTCTGGTGGCAAAGAGATGTTTCAACCCTAAATTTTTTGTCAAATGTGGCCAATCTAGCTAGTAGCCACTTTCACAAAGAAAGTATCTTTGGTTTGTGCTATAGGAAGTTTCAAATCTAGTTATCCAGAAGTTTGAAATCAATTTTACTTGGATTATTATTTATTACTCAAAATGTTGCAGGCAAACCCATTTCCGTTTGTGTGCCAAATCTGGAGACTTTGGTTTGTTTAATACTTTTATCTCTTTTGTGCAGGCTTCTGAGTTGCAGTTTGAGTTCCTTTGCTCTTACCTTGCTGAGCTCAGTTTGTTGGATTATAATTGTGTGAAGTTCTTGCCTTCTATGGTGGCTGCATCAGTTGTATTTCTTGCTCGTTTTATGTTAAGCCCGAAGACTCATCCTTGGGTAATGTTTTAATTCGTGTACAATCTATATTTGGTATTTGACTTTGACACAGTTACTAAAATATTTCCATTTGATTACAGAATTCAGCCATCTATGAATTCACCAGATATAAACCTGCAGACTTGAAGGAATGTGTTCTAAATATACATGACTTGTACCTTGGTAGGAAAGGCGGCTCTTTGCAAGCTGTGAGAGATAAATACAAACAGCATAAGGTGATATTCCTGCACGCTTATTGTCAATCCTTTTTTACCAAACAAACTTAATTTGTCCTACCTTGGCCCAAGCTTTTTAAACAATTGAGTTATTTATTTCATTACACTTGTCACATCTGATGCATTTATGCTGATTCCATTTGTTGTTTTGGTCACAGTTCAAATGCATGGCAACAACACCCTCTCCCCCGGAGATATCTCTTTCTTTTTTTGAATTTAGAGGGGCTGATCCTTAG
Protein sequence

>MS.gene044085.t1

MEPKSVNPTRRPQREAKKKAVAALCEQQRKRKRVPLGEITNDVITNANDVVLPTQKKRNVATSSNSDETPVPEKLEKFEDPQLCETYVSDIYDYLRNMEVDSSKRPLCDYIQKVQRDVNASMRGVLVDWLVEVAEEYKLVSDTLYFSVSYIDRFLSLNDLTRQKLQLLGVASMLVASKYEEIKPPEVEDFCYITDNTYSKEEVLTMEADILKSLKFELGGPTIKTFLRRFITKVGLEGVDASELQFEFLCSYLAELSLLDYNCVKFLPSMVAASVVFLARFMLSPKTHPWNSAIYEFTRYKPADLKECVLNIHDLYLGRKGGSLQAVRDKYKQHKFKCMATTPSPPEISLSFFEFRGADP