AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24450.t1 MTR_1g018680 94.262 366 12 3 1 360 1 363 0.0 674
MS.gene24450.t1 MTR_3g102520 55.325 338 135 5 30 354 23 357 2.07e-125 365
MS.gene24450.t1 MTR_3g102530 54.755 347 145 4 16 353 6 349 3.31e-123 359
MS.gene24450.t1 MTR_3g102510 54.294 326 138 5 30 354 23 338 7.38e-117 342
MS.gene24450.t1 MTR_3g102560 53.824 340 137 5 14 353 5 324 4.01e-115 337
MS.gene24450.t1 MTR_3g088415 53.597 278 124 3 78 354 231 504 2.44e-104 316
MS.gene24450.t1 MTR_3g102540 55.039 258 109 2 91 348 13 263 7.04e-97 289
MS.gene24450.t1 MTR_3g088405 49.281 278 136 3 79 354 235 509 3.37e-94 290
MS.gene24450.t1 MTR_3g107970 51.273 275 131 2 76 350 202 473 1.33e-92 285
MS.gene24450.t1 MTR_2g102520 51.111 270 127 3 78 345 206 472 3.70e-92 284
MS.gene24450.t1 MTR_1g011470 50.368 272 131 3 77 347 151 419 3.87e-89 274
MS.gene24450.t1 MTR_2g102550 47.273 275 142 2 71 345 151 422 4.05e-87 270
MS.gene24450.t1 MTR_3g088415 57.944 214 85 3 78 290 231 440 3.45e-84 262
MS.gene24450.t1 MTR_3g088415 59.064 171 66 2 78 248 231 397 5.88e-67 216
MS.gene24450.t1 MTR_7g089080 39.689 257 150 4 87 343 199 450 7.77e-54 191
MS.gene24450.t1 MTR_5g088980 38.211 246 141 5 87 331 165 400 1.44e-49 172
MS.gene24450.t1 MTR_8g074000 35.827 254 153 4 87 340 176 419 5.25e-49 171
MS.gene24450.t1 MTR_5g023790 36.015 261 157 4 80 340 165 415 8.98e-48 167
MS.gene24450.t1 MTR_5g023790 39.801 201 112 3 80 280 165 356 1.11e-43 155
MS.gene24450.t1 MTR_5g023790 42.105 152 85 2 80 231 165 313 2.97e-35 132
MS.gene24450.t1 MTR_3g060410 33.333 231 141 6 118 340 131 356 2.76e-25 105
MS.gene24450.t1 MTR_3g060770 33.937 221 129 6 118 333 56 264 8.47e-23 97.1
MS.gene24450.t1 MTR_3g060820 35.714 196 116 5 118 308 46 236 5.22e-22 94.7
MS.gene24450.t1 MTR_3g061680 36.224 196 115 5 118 308 46 236 6.30e-22 94.4
MS.gene24450.t1 MTR_3g060400 32.035 231 144 6 118 340 52 277 1.01e-21 94.0
MS.gene24450.t1 MTR_3g460990 32.035 231 144 6 118 340 52 277 1.01e-21 94.0
MS.gene24450.t1 MTR_5g044040 46.667 75 40 0 144 218 7 81 8.18e-19 82.0
MS.gene24450.t1 MTR_1g032850 30.366 191 120 6 123 309 372 553 4.36e-15 76.6
MS.gene24450.t1 MTR_1g107535 29.167 216 107 9 73 277 83 263 6.76e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24450.t1 AT5G43080 60.000 350 133 4 7 354 7 351 1.25e-146 419
MS.gene24450.t1 AT1G47230 57.895 361 134 5 7 354 6 361 2.93e-144 413
MS.gene24450.t1 AT5G43080 59.143 350 131 5 7 354 7 346 9.32e-143 409
MS.gene24450.t1 AT1G47230 57.735 362 134 6 7 354 6 362 2.39e-142 408
MS.gene24450.t1 AT1G47210 56.056 355 139 4 16 354 15 368 3.16e-137 395
MS.gene24450.t1 AT1G47220 56.711 298 120 2 57 354 34 322 9.19e-117 342
MS.gene24450.t1 AT1G44110 54.545 275 120 3 80 353 187 457 2.61e-102 309
MS.gene24450.t1 AT5G25380 50.719 278 126 3 79 352 165 435 7.21e-97 295
MS.gene24450.t1 AT5G25380 50.719 278 126 3 79 352 165 435 7.21e-97 295
MS.gene24450.t1 AT5G25380 50.719 278 126 3 79 352 171 441 1.72e-96 294
MS.gene24450.t1 AT5G11300 44.602 352 181 6 10 352 88 434 3.47e-95 290
MS.gene24450.t1 AT1G80370 53.053 262 120 2 79 340 189 447 1.43e-92 285
MS.gene24450.t1 AT1G15570 52.060 267 125 2 79 345 180 443 5.93e-92 283
MS.gene24450.t1 AT5G25380 50.958 261 117 3 79 335 165 418 1.81e-91 280
MS.gene24450.t1 AT1G77390 48.175 274 139 2 80 353 171 441 2.53e-88 273
MS.gene24450.t1 AT5G11300 44.407 295 150 6 10 295 88 377 1.43e-76 242
MS.gene24450.t1 AT3G11520 36.145 332 192 7 17 340 82 401 2.46e-51 176
MS.gene24450.t1 AT3G11520 38.047 297 164 7 17 305 82 366 8.83e-50 171
MS.gene24450.t1 AT5G06150 37.970 266 151 6 87 351 182 434 3.45e-49 171
MS.gene24450.t1 AT2G26760 37.970 266 154 5 87 351 130 385 4.49e-46 162
MS.gene24450.t1 AT1G20610 34.211 266 164 5 80 345 170 424 1.19e-45 162
MS.gene24450.t1 AT1G47210 48.876 178 75 3 16 177 15 192 3.25e-45 154
MS.gene24450.t1 AT4G37490 37.262 263 148 8 87 343 165 416 1.40e-44 159
MS.gene24450.t1 AT1G76310 34.902 255 155 5 87 340 177 421 2.05e-43 155
MS.gene24450.t1 AT2G17620 37.354 257 145 5 87 340 171 414 3.02e-42 152
MS.gene24450.t1 AT1G16330 33.457 269 166 5 66 331 371 629 1.21e-41 154
MS.gene24450.t1 AT4G35620 38.039 255 148 5 87 341 172 416 1.23e-41 151
MS.gene24450.t1 AT1G76310 37.879 198 113 4 87 283 177 365 1.22e-39 144
MS.gene24450.t1 AT1G76310 31.802 283 154 6 87 340 177 449 9.80e-38 141
MS.gene24450.t1 AT4G35620 40.580 207 113 5 87 293 172 368 1.04e-36 137
MS.gene24450.t1 AT5G06150 43.448 145 78 1 87 231 182 322 1.04e-35 133
MS.gene24450.t1 AT1G76310 41.781 146 81 3 87 231 177 319 1.58e-32 124
MS.gene24450.t1 AT1G20590 34.463 177 105 3 151 323 66 235 8.48e-25 102
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 1.99e-17 84.0
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.30e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 269 389 2.30e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 269 389 2.30e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.37e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.37e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.37e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 272 392 2.37e-17 83.6
MS.gene24450.t1 AT1G34460 33.103 145 73 2 87 231 258 378 2.45e-17 83.6
MS.gene24450.t1 AT1G14750 31.343 201 126 7 122 319 218 409 1.24e-14 75.1
MS.gene24450.t1 AT1G14750 27.969 261 169 8 65 319 163 410 1.31e-14 75.1
MS.gene24450.t1 AT1G14750 27.969 261 169 8 65 319 331 578 1.48e-14 75.5
MS.gene24450.t1 AT1G14750 30.366 191 123 5 122 309 386 569 7.02e-14 73.2
MS.gene24450.t1 AT1G70210 30.726 179 111 5 106 276 64 237 8.03e-11 63.2

Find 67 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGAAGTTCTTGCCTTCTA+TGG 0.257368 1.1:-4304073 MS.gene24450:CDS
CGCGTGGGGTTAACGCTCTT+TGG 0.262421 1.1:+4306218 None:intergenic
CTTAGGAATGTCTTTATTGT+AGG 0.290255 1.1:+4305034 None:intergenic
CCTTGCTGAGCTCAGTTTGT+TGG 0.292382 1.1:-4304106 MS.gene24450:CDS
CAAGTCTGCAGGTTTATATC+TGG 0.333404 1.1:+4303889 None:intergenic
CTCCGATGAAACTCCGTTAC+CGG 0.335792 1.1:-4306030 MS.gene24450:CDS
GAAGTCATTGAAGTTTGAAT+TGG 0.360491 1.1:-4305062 MS.gene24450:CDS
TGAGGGGTGTTCTTGTTGAT+TGG 0.376649 1.1:-4305754 MS.gene24450:CDS
ATATAAACCTGCAGACTTGA+AGG 0.399847 1.1:-4303885 MS.gene24450:CDS
ACTGATGCAGCCACCATAGA+AGG 0.402548 1.1:+4304060 None:intergenic
ATATCTGGTGAATTCATAGA+TGG 0.420121 1.1:+4303904 None:intergenic
GGGATGTTAATGCGAGTATG+AGG 0.420433 1.1:-4305772 MS.gene24450:CDS
GTTATGTGAAACATATGTTT+CGG 0.423499 1.1:-4305979 MS.gene24450:CDS
TTACCCAAGGATGAGTCTTC+GGG 0.425884 1.1:+4304010 None:intergenic
ATTACCCAAGGATGAGTCTT+CGG 0.430153 1.1:+4304009 None:intergenic
TTTAGGTTGATTCTAGTAAG+AGG 0.436695 1.1:-4305826 MS.gene24450:intron
TCACGTTGAGGGCGGCGCGT+GGG 0.441478 1.1:+4306203 None:intergenic
TACAAGATTCACCTCTTCCT+TGG 0.443619 1.1:+4305205 None:intergenic
AGAAGATTCATCACTAAAGT+TGG 0.459150 1.1:-4304918 MS.gene24450:CDS
ACACATTCCTTCAAGTCTGC+AGG 0.460260 1.1:+4303878 None:intergenic
TTCCGGTAACGGAGTTTCAT+CGG 0.464668 1.1:+4306028 None:intergenic
AAGTCATTGAAGTTTGAATT+GGG 0.468127 1.1:-4305061 MS.gene24450:CDS
GGGTGTTCTTGTTGATTGGT+TGG 0.469360 1.1:-4305750 MS.gene24450:CDS
CACGTTGAGGGCGGCGCGTG+GGG 0.493799 1.1:+4306204 None:intergenic
GATTAAGCCACCTGAGGTTG+AGG 0.495688 1.1:-4305258 MS.gene24450:CDS
AGATAAATACAAACAGCATA+AGG 0.498310 1.1:-4303798 MS.gene24450:intron
AAACGAAAACGCGTTCCTCT+TGG 0.503110 1.1:-4306137 MS.gene24450:CDS
AATATACATGACTTGTACCT+TGG 0.507114 1.1:-4303851 MS.gene24450:CDS
TTGTTCACATAGTGCAGCTA+CGG 0.511501 1.1:+4306166 None:intergenic
TTCACGTTGAGGGCGGCGCG+TGG 0.513771 1.1:+4306202 None:intergenic
GGCAACAACACCCTCTCCCC+CGG 0.520065 1.1:-4303607 MS.gene24450:CDS
CTTCAAAATATCAGCCTCCA+TGG 0.525850 1.1:+4305081 None:intergenic
TTTCGGTTTCTTATATCGAT+CGG 0.529661 1.1:-4305682 MS.gene24450:CDS
ATCACTAAAGTTGGTCTAGA+GGG 0.532834 1.1:-4304909 MS.gene24450:CDS
AAGCCCGAAGACTCATCCTT+GGG 0.535304 1.1:-4304013 MS.gene24450:intron
TAACAAAAGTCCTCAACCTC+AGG 0.540151 1.1:+4305248 None:intergenic
GGATGTTAATGCGAGTATGA+GGG 0.542531 1.1:-4305771 MS.gene24450:CDS
GAAGTTCTTGCCTTCTATGG+TGG 0.548750 1.1:-4304070 MS.gene24450:CDS
TAAGCCCGAAGACTCATCCT+TGG 0.550073 1.1:-4304014 MS.gene24450:CDS
ACTCGGTGTTGCTTCGATGT+TGG 0.553532 1.1:-4305618 MS.gene24450:CDS
TAAAACGACGTCGTTTGCGT+TGG 0.560022 1.1:+4306091 None:intergenic
GACTTGTACCTTGGTAGGAA+AGG 0.582896 1.1:-4303842 MS.gene24450:CDS
AGACAGAAGTTGCAATTACT+CGG 0.583486 1.1:-4305635 MS.gene24450:CDS
CAATACATACTCCAAGGAAG+AGG 0.589667 1.1:-4305216 MS.gene24450:intron
CATCACTAAAGTTGGTCTAG+AGG 0.599941 1.1:-4304910 MS.gene24450:CDS
TCGTTTGTTATTTCACCAAG+AGG 0.602061 1.1:+4306122 None:intergenic
GCTCAGCAAGGTAAGAGCAA+AGG 0.603798 1.1:+4304118 None:intergenic
TTGGTCACAGTTCAAATGCG+TGG 0.611335 1.1:-4303628 MS.gene24450:intron
TACATGACTTGTACCTTGGT+AGG 0.612523 1.1:-4303847 MS.gene24450:CDS
CTACAATAAAGACATTCCTA+AGG 0.613211 1.1:-4305033 MS.gene24450:intron
CAAAAGTCCTCAACCTCAGG+TGG 0.618973 1.1:+4305251 None:intergenic
GATGTTAATGCGAGTATGAG+GGG 0.627184 1.1:-4305770 MS.gene24450:CDS
TCATTGAAGTTTGAATTGGG+TGG 0.631240 1.1:-4305058 MS.gene24450:CDS
TCTTTGCTTCACGTTGAGGG+CGG 0.632157 1.1:+4306195 None:intergenic
TACGGACAATACATACTCCA+AGG 0.633308 1.1:-4305222 MS.gene24450:CDS
TTGTACCTTGGTAGGAAAGG+CGG 0.635313 1.1:-4303839 MS.gene24450:CDS
ATTATATACAGAAAGTTCAG+AGG 0.639886 1.1:-4305793 MS.gene24450:CDS
ATACGATTATCTTCGTAACA+TGG 0.640847 1.1:-4305952 MS.gene24450:CDS
AAGAGCCGCCTTTCCTACCA+AGG 0.647189 1.1:+4303834 None:intergenic
TTATATACAGAAAGTTCAGA+GGG 0.657090 1.1:-4305792 MS.gene24450:CDS
CCAACAAACTGAGCTCAGCA+AGG 0.668066 1.1:+4304106 None:intergenic
TTCTGTATATAATCACACAA+TGG 0.674639 1.1:+4305803 None:intergenic
AAAGAAAGAGATATCTCCGG+GGG 0.686776 1.1:+4303591 None:intergenic
TGAAGAGATTAAGCCACCTG+AGG 0.697941 1.1:-4305264 MS.gene24450:CDS
ACATATGTTTCACATAACTG+AGG 0.706101 1.1:+4305984 None:intergenic
CGATTATCTTCGTAACATGG+AGG 0.706739 1.1:-4305949 MS.gene24450:intron
AAAAGAAAGAGATATCTCCG+GGG 0.745816 1.1:+4303590 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGATTTTTTATTTGTTT+TGG - chr1.1:4304488-4304507 MS.gene24450:intron 10.0%
!!! TTTTTTTTTTCTTTGTTTTT+AGG - chr1.1:4303944-4303963 MS.gene24450:intron 10.0%
!! TTAACTTCAAATTTCAATTA+GGG - chr1.1:4303879-4303898 MS.gene24450:CDS 15.0%
!!! TTTAACTTCAAATTTCAATT+AGG - chr1.1:4303878-4303897 MS.gene24450:CDS 15.0%
!!! TTTCTTTTTTTGAATTTAGA+GGG - chr1.1:4306212-4306231 MS.gene24450:CDS 15.0%
!! AATTAAAATAACACTCACAT+TGG - chr1.1:4304289-4304308 MS.gene24450:intron 20.0%
!! AGTAATCTCTTTGTTAAAAT+GGG + chr1.1:4304365-4304384 None:intergenic 20.0%
!! ATTCATGTACAATCTATATT+TGG - chr1.1:4305807-4305826 MS.gene24450:CDS 20.0%
!! CACATTTGACAAAAAATTTA+GGG + chr1.1:4305425-4305444 None:intergenic 20.0%
!! TGATGAATAAATACCTAATT+TGG - chr1.1:4304242-4304261 MS.gene24450:intron 20.0%
!!! AAATAGTATCATCTAAGATA+GGG - chr1.1:4305072-4305091 MS.gene24450:CDS 20.0%
!!! AGTTTGAAATCAATTTTACT+TGG - chr1.1:4305519-4305538 MS.gene24450:intron 20.0%
!!! ATGTGAGTGTTATTTTAATT+TGG + chr1.1:4304289-4304308 None:intergenic 20.0%
!!! CTTTCTTTTTTTGAATTTAG+AGG - chr1.1:4306211-4306230 MS.gene24450:CDS 20.0%
!!! GTTTTGATTTTCCATAATTT+GGG - chr1.1:4303901-4303920 MS.gene24450:CDS 20.0%
!!! TAAAATTGATTTCAAACTTC+TGG + chr1.1:4305517-4305536 None:intergenic 20.0%
!!! TGTGAGTGTTATTTTAATTT+GGG + chr1.1:4304288-4304307 None:intergenic 20.0%
!!! TTAAGTTTGTTTGGTAAAAA+AGG + chr1.1:4306042-4306061 None:intergenic 20.0%
!!! TTCAGATACACTTTATTTTT+CGG - chr1.1:4304088-4304107 MS.gene24450:CDS 20.0%
!!! TTCTTTTTTTGAATTTAGAG+GGG - chr1.1:4306213-4306232 MS.gene24450:CDS 20.0%
!!! TTGGTATTTTGTTTGATTAA+AGG - chr1.1:4304677-4304696 MS.gene24450:intron 20.0%
!!! TTTTGATTTTCCATAATTTG+GGG - chr1.1:4303902-4303921 MS.gene24450:CDS 20.0%
! AAAAAAGAAAGAGATATCTC+CGG + chr1.1:4306202-4306221 None:intergenic 25.0%
! AAATAGAAAGAGAATTAGAG+TGG - chr1.1:4305190-4305209 MS.gene24450:intron 25.0%
! AAATGGGTCAAAATTAATAG+AGG + chr1.1:4304349-4304368 None:intergenic 25.0%
! AAGTCATTGAAGTTTGAATT+GGG - chr1.1:4304726-4304745 MS.gene24450:intron 25.0%
! AATAGAAAGAGAATTAGAGT+GGG - chr1.1:4305191-4305210 MS.gene24450:intron 25.0%
! ACTCAATTGTTTAAAAAGCT+TGG + chr1.1:4306081-4306100 None:intergenic 25.0%
! AGATAAATACAAACAGCATA+AGG - chr1.1:4305989-4306008 MS.gene24450:CDS 25.0%
! ATGAATTAAAACATTACCCA+AGG + chr1.1:4305793-4305812 None:intergenic 25.0%
! ATGTTTCTTTGTATGTTGAA+TGG - chr1.1:4304796-4304815 MS.gene24450:intron 25.0%
! ATTACTCTTATGAAAATGTC+AGG - chr1.1:4304379-4304398 MS.gene24450:intron 25.0%
! ATTATATACAGAAAGTTCAG+AGG - chr1.1:4303994-4304013 MS.gene24450:intron 25.0%
! CATTCTTAAAAAAGTTAGCA+AGG + chr1.1:4305365-4305384 None:intergenic 25.0%
! CCACATTTGACAAAAAATTT+AGG + chr1.1:4305426-4305445 None:intergenic 25.0%
! CTCAATTGTTTAAAAAGCTT+GGG + chr1.1:4306080-4306099 None:intergenic 25.0%
! GAAAATGTCAGGAATTAATT+TGG - chr1.1:4304390-4304409 MS.gene24450:intron 25.0%
! GAGTAATCTCTTTGTTAAAA+TGG + chr1.1:4304366-4304385 None:intergenic 25.0%
! GTTATGTGAAACATATGTTT+CGG - chr1.1:4303808-4303827 MS.gene24450:CDS 25.0%
! TTATATACAGAAAGTTCAGA+GGG - chr1.1:4303995-4304014 MS.gene24450:intron 25.0%
! TTCTGTATATAATCACACAA+TGG + chr1.1:4303987-4304006 None:intergenic 25.0%
! TTTATTACTCAAAATGTTGC+AGG - chr1.1:4305549-4305568 MS.gene24450:intron 25.0%
!! AAATTGTTGCATCTAAGATA+TGG - chr1.1:4305134-4305153 MS.gene24450:intron 25.0%
!! CAAATAGTATCATCTAAGAT+AGG - chr1.1:4305071-4305090 MS.gene24450:CDS 25.0%
!! CAGGAATTAATTTGGTATAT+TGG - chr1.1:4304398-4304417 MS.gene24450:intron 25.0%
!! TAGGACAAATTAAGTTTGTT+TGG + chr1.1:4306051-4306070 None:intergenic 25.0%
!!! CCTAAATTTTTTGTCAAATG+TGG - chr1.1:4305423-4305442 MS.gene24450:intron 25.0%
!!! GGTTTTGATTTTCCATAATT+TGG - chr1.1:4303900-4303919 MS.gene24450:CDS 25.0%
!!! TTTCACAAAGAAAGTATCTT+TGG - chr1.1:4305459-4305478 MS.gene24450:intron 25.0%
AAAAAGAAAGAGATATCTCC+GGG + chr1.1:4306201-4306220 None:intergenic 30.0%
AACCTATAACTACTATGAGA+TGG + chr1.1:4304933-4304952 None:intergenic 30.0%
AATTTGGGTGCAAACAAATT+GGG + chr1.1:4304273-4304292 None:intergenic 30.0%
ACATATGTTTCACATAACTG+AGG + chr1.1:4303806-4303825 None:intergenic 30.0%
ACCTATAACTACTATGAGAT+GGG + chr1.1:4304932-4304951 None:intergenic 30.0%
AGAAGATTCATCACTAAAGT+TGG - chr1.1:4304869-4304888 MS.gene24450:intron 30.0%
AGATACTTTCTTTGTGAAAG+TGG + chr1.1:4305458-4305477 None:intergenic 30.0%
AGTAGTTATAGGTTATGACA+TGG - chr1.1:4304939-4304958 MS.gene24450:intron 30.0%
ATACGATTATCTTCGTAACA+TGG - chr1.1:4303835-4303854 MS.gene24450:CDS 30.0%
ATATCTGGTGAATTCATAGA+TGG + chr1.1:4305886-4305905 None:intergenic 30.0%
CTACAATAAAGACATTCCTA+AGG - chr1.1:4304754-4304773 MS.gene24450:intron 30.0%
CTTAGGAATGTCTTTATTGT+AGG + chr1.1:4304756-4304775 None:intergenic 30.0%
GAAGTCATTGAAGTTTGAAT+TGG - chr1.1:4304725-4304744 MS.gene24450:intron 30.0%
TAATTTGGGTGCAAACAAAT+TGG + chr1.1:4304274-4304293 None:intergenic 30.0%
TACAGACTAAAATCACATCT+TGG + chr1.1:4304446-4304465 None:intergenic 30.0%
TATAGGTTATGACATGGATT+CGG - chr1.1:4304945-4304964 MS.gene24450:intron 30.0%
TATTGAATGAACTATCCAGA+AGG + chr1.1:4305285-4305304 None:intergenic 30.0%
TGGACTATAAACAAATTGTG+AGG - chr1.1:4304418-4304437 MS.gene24450:intron 30.0%
TTTCGGTTTCTTATATCGAT+CGG - chr1.1:4304105-4304124 MS.gene24450:CDS 30.0%
TTTGTTGCATTCTATAATGG+TGG - chr1.1:4304312-4304331 MS.gene24450:intron 30.0%
! AATATACATGACTTGTACCT+TGG - chr1.1:4305936-4305955 MS.gene24450:intron 30.0%
! ATAGTTGAACTTTGAGCTTT+TGG - chr1.1:4305236-4305255 MS.gene24450:CDS 30.0%
! ATGTTCAACTTTTAGTAGTG+TGG - chr1.1:4304983-4305002 MS.gene24450:intron 30.0%
! GATTTTGGATGGTATTGTTT+TGG - chr1.1:4304658-4304677 MS.gene24450:intron 30.0%
! TACTTTTATCTCTTTTGTGC+AGG - chr1.1:4305624-4305643 MS.gene24450:CDS 30.0%
! TAGTTGAACTTTGAGCTTTT+GGG - chr1.1:4305237-4305256 MS.gene24450:CDS 30.0%
! TCGAATTTCTGTAACTTTTC+CGG + chr1.1:4303779-4303798 None:intergenic 30.0%
! TGAGGACTTTTGTTACATTA+CGG - chr1.1:4304547-4304566 MS.gene24450:intron 30.0%
! TGTGTTGTTGAATGTTTGTT+TGG - chr1.1:4304621-4304640 MS.gene24450:intron 30.0%
! TGTTCAACTTTTAGTAGTGT+GGG - chr1.1:4304984-4305003 MS.gene24450:intron 30.0%
!! AAACTTAATTTGTCCTACCT+TGG - chr1.1:4306054-4306073 MS.gene24450:CDS 30.0%
!! GTTGAATGTTTGTTTGGTTT+TGG - chr1.1:4304627-4304646 MS.gene24450:intron 30.0%
!! TGGTTTGTTGCATTCTATAA+TGG - chr1.1:4304309-4304328 MS.gene24450:intron 30.0%
!! TTGATTAAAGGTTTTGACCA+TGG - chr1.1:4304689-4304708 MS.gene24450:intron 30.0%
!! TTTAGGTTGATTCTAGTAAG+AGG - chr1.1:4303961-4303980 MS.gene24450:intron 30.0%
AAAAAGTTAGCAAGGTTGCA+TGG + chr1.1:4305357-4305376 None:intergenic 35.0%
AAAAGAAAGAGATATCTCCG+GGG + chr1.1:4306200-4306219 None:intergenic 35.0%
AAAAGTTAGCAAGGTTGCAT+GGG + chr1.1:4305356-4305375 None:intergenic 35.0%
AAGCAAAAAGCCCCAAATTA+TGG + chr1.1:4303915-4303934 None:intergenic 35.0%
ACCCATCTCATAGTAGTTAT+AGG - chr1.1:4304928-4304947 MS.gene24450:intron 35.0%
ACTGTGACCAAAACAACAAA+TGG + chr1.1:4306150-4306169 None:intergenic 35.0%
AGACAGAAGTTGCAATTACT+CGG - chr1.1:4304152-4304171 MS.gene24450:intron 35.0%
ATATAAACCTGCAGACTTGA+AGG - chr1.1:4305902-4305921 MS.gene24450:intron 35.0%
CAATAAAACACACATGCCTT+AGG + chr1.1:4304773-4304792 None:intergenic 35.0%
GGCTGAATTCTGTAATCAAA+TGG + chr1.1:4305865-4305884 None:intergenic 35.0%
TCATTGAAGTTTGAATTGGG+TGG - chr1.1:4304729-4304748 MS.gene24450:intron 35.0%
! ATCACTAAAGTTGGTCTAGA+GGG - chr1.1:4304878-4304897 MS.gene24450:intron 35.0%
! GGGGCTTTTTGCTTAATTTA+GGG - chr1.1:4303921-4303940 MS.gene24450:intron 35.0%
! TGGGGCTTTTTGCTTAATTT+AGG - chr1.1:4303920-4303939 MS.gene24450:intron 35.0%
! TTCTGTAACTTTTCCGGTAA+CGG + chr1.1:4303773-4303792 None:intergenic 35.0%
! TTTTAGTAGTGTGGGACTTT+TGG - chr1.1:4304992-4305011 MS.gene24450:intron 35.0%
! TTTTCTTTGCTTCACGTTGA+GGG + chr1.1:4303598-4303617 None:intergenic 35.0%
! TTTTTCTTTGCTTCACGTTG+AGG + chr1.1:4303599-4303618 None:intergenic 35.0%
!! ATTAAAGGTTTTGACCATGG+AGG - chr1.1:4304692-4304711 MS.gene24450:intron 35.0%
!! GTATCTTTGGTTTGTGCTAT+AGG - chr1.1:4305472-4305491 MS.gene24450:intron 35.0%
!! TCGTTTGTTATTTCACCAAG+AGG + chr1.1:4303668-4303687 None:intergenic 35.0%
!!! GCTGATTCCATTTGTTGTTT+TGG - chr1.1:4306140-4306159 MS.gene24450:CDS 35.0%
!!! GTTACTTTTGCTATTGTGTG+TGG - chr1.1:4305014-4305033 MS.gene24450:intron 35.0%
!!! GTTTTGGATCTTGCTGATTT+TGG - chr1.1:4304643-4304662 MS.gene24450:intron 35.0%
AAAGAAAGAGATATCTCCGG+GGG + chr1.1:4306199-4306218 None:intergenic 40.0%
AAATTGGGAGGGACCAAATT+AGG + chr1.1:4304258-4304277 None:intergenic 40.0%
ATTACCCAAGGATGAGTCTT+CGG + chr1.1:4305781-4305800 None:intergenic 40.0%
CAAACCAAAGTCTCCAGATT+TGG + chr1.1:4305599-4305618 None:intergenic 40.0%
CAAGTCTGCAGGTTTATATC+TGG + chr1.1:4305901-4305920 None:intergenic 40.0%
CAATACATACTCCAAGGAAG+AGG - chr1.1:4304571-4304590 MS.gene24450:intron 40.0%
CCAGATTTGGCACACATAAA+CGG + chr1.1:4305586-4305605 None:intergenic 40.0%
CGATTATCTTCGTAACATGG+AGG - chr1.1:4303838-4303857 MS.gene24450:CDS 40.0%
CTTCAAAATATCAGCCTCCA+TGG + chr1.1:4304709-4304728 None:intergenic 40.0%
CTTTGTGAAAGTGGCTAGAT+TGG + chr1.1:4305449-4305468 None:intergenic 40.0%
GATGTTAATGCGAGTATGAG+GGG - chr1.1:4304017-4304036 MS.gene24450:CDS 40.0%
GGATGTTAATGCGAGTATGA+GGG - chr1.1:4304016-4304035 MS.gene24450:CDS 40.0%
TAACAAAAGTCCTCAACCTC+AGG + chr1.1:4304542-4304561 None:intergenic 40.0%
TACAAGATTCACCTCTTCCT+TGG + chr1.1:4304585-4304604 None:intergenic 40.0%
TACGGACAATACATACTCCA+AGG - chr1.1:4304565-4304584 MS.gene24450:intron 40.0%
TGGCACACATAAACGGAAAT+GGG + chr1.1:4305579-4305598 None:intergenic 40.0%
TGTGAAGTTCTTGCCTTCTA+TGG - chr1.1:4305714-4305733 MS.gene24450:CDS 40.0%
TGTTTAAAAAGCTTGGGCCA+AGG + chr1.1:4306074-4306093 None:intergenic 40.0%
TTGGCACACATAAACGGAAA+TGG + chr1.1:4305580-4305599 None:intergenic 40.0%
TTGTTCACATAGTGCAGCTA+CGG + chr1.1:4303624-4303643 None:intergenic 40.0%
! CATCACTAAAGTTGGTCTAG+AGG - chr1.1:4304877-4304896 MS.gene24450:intron 40.0%
! CATCTAAGATAGGGAGATTG+TGG - chr1.1:4305081-4305100 MS.gene24450:CDS 40.0%
! GTTAGTCTGCAGAACAAGAT+AGG + chr1.1:4305329-4305348 None:intergenic 40.0%
! TACATGACTTGTACCTTGGT+AGG - chr1.1:4305940-4305959 MS.gene24450:intron 40.0%
! TTGAACTTTGAGCTTTTGGG+AGG - chr1.1:4305240-4305259 MS.gene24450:CDS 40.0%
!! GTGGGTTTTGTCATGTCATT+TGG - chr1.1:4305209-4305228 MS.gene24450:intron 40.0%
!! TGAACTTTGAGCTTTTGGGA+GGG - chr1.1:4305241-4305260 MS.gene24450:CDS 40.0%
!!! ACGTTTCGTTTTTTCTGCGT+TGG + chr1.1:4303722-4303741 None:intergenic 40.0%
!!! TGGATCTTGCTGATTTTGGA+TGG - chr1.1:4304647-4304666 MS.gene24450:intron 40.0%
AAACGAAAACGCGTTCCTCT+TGG - chr1.1:4303650-4303669 MS.gene24450:intron 45.0%
ACACATTCCTTCAAGTCTGC+AGG + chr1.1:4305912-4305931 None:intergenic 45.0%
AGGATTGACAATAAGCGTGC+AGG + chr1.1:4306022-4306041 None:intergenic 45.0%
CCGTTTATGTGTGCCAAATC+TGG - chr1.1:4305583-4305602 MS.gene24450:intron 45.0%
GAAGTTCTTGCCTTCTATGG+TGG - chr1.1:4305717-4305736 MS.gene24450:CDS 45.0%
GACTTGTACCTTGGTAGGAA+AGG - chr1.1:4305945-4305964 MS.gene24450:intron 45.0%
GAGATGGGAATTGAACCTTC+TGG - chr1.1:4305267-4305286 MS.gene24450:CDS 45.0%
GGGATGTTAATGCGAGTATG+AGG - chr1.1:4304015-4304034 MS.gene24450:CDS 45.0%
TAAAAAGCTTGGGCCAAGGT+AGG + chr1.1:4306070-4306089 None:intergenic 45.0%
TAAAACGACGTCGTTTGCGT+TGG + chr1.1:4303699-4303718 None:intergenic 45.0%
TGAAGAGATTAAGCCACCTG+AGG - chr1.1:4304523-4304542 MS.gene24450:intron 45.0%
TGGGTGCAAACAAATTGGGA+GGG + chr1.1:4304269-4304288 None:intergenic 45.0%
TGTGCCAAATCTGGAGACTT+TGG - chr1.1:4305592-4305611 MS.gene24450:intron 45.0%
TTACCCAAGGATGAGTCTTC+GGG + chr1.1:4305780-4305799 None:intergenic 45.0%
TTGGGTGCAAACAAATTGGG+AGG + chr1.1:4304270-4304289 None:intergenic 45.0%
TTGGTCACAGTTCAAATGCG+TGG - chr1.1:4306159-4306178 MS.gene24450:CDS 45.0%
TTGTACCTTGGTAGGAAAGG+CGG - chr1.1:4305948-4305967 MS.gene24450:intron 45.0%
! GGGTGTTCTTGTTGATTGGT+TGG - chr1.1:4304037-4304056 MS.gene24450:CDS 45.0%
!! TGAGGGGTGTTCTTGTTGAT+TGG - chr1.1:4304033-4304052 MS.gene24450:CDS 45.0%
!! TTCCGGTAACGGAGTTTCAT+CGG + chr1.1:4303762-4303781 None:intergenic 45.0%
AAGCCCGAAGACTCATCCTT+GGG - chr1.1:4305774-4305793 MS.gene24450:CDS 50.0%
ACTGATGCAGCCACCATAGA+AGG + chr1.1:4305730-4305749 None:intergenic 50.0%
CAAAAGTCCTCAACCTCAGG+TGG + chr1.1:4304539-4304558 None:intergenic 50.0%
CCAACAAACTGAGCTCAGCA+AGG + chr1.1:4305684-4305703 None:intergenic 50.0%
CCTTGCTGAGCTCAGTTTGT+TGG - chr1.1:4305681-4305700 MS.gene24450:CDS 50.0%
CTCCGATGAAACTCCGTTAC+CGG - chr1.1:4303757-4303776 MS.gene24450:intron 50.0%
GATTAAGCCACCTGAGGTTG+AGG - chr1.1:4304529-4304548 MS.gene24450:intron 50.0%
GCTCAGCAAGGTAAGAGCAA+AGG + chr1.1:4305672-4305691 None:intergenic 50.0%
TAAGCCCGAAGACTCATCCT+TGG - chr1.1:4305773-4305792 MS.gene24450:CDS 50.0%
TCTTTGCTTCACGTTGAGGG+CGG + chr1.1:4303595-4303614 None:intergenic 50.0%
!! ACTCGGTGTTGCTTCGATGT+TGG - chr1.1:4304169-4304188 MS.gene24450:intron 50.0%
!! CTTTTGGGAGGGTCTGAGAT+GGG - chr1.1:4305252-4305271 MS.gene24450:CDS 50.0%
AAGAGATATCTCCGGGGGAG+AGG + chr1.1:4306194-4306213 None:intergenic 55.0%
AAGAGCCGCCTTTCCTACCA+AGG + chr1.1:4305956-4305975 None:intergenic 55.0%
AGAGATATCTCCGGGGGAGA+GGG + chr1.1:4306193-4306212 None:intergenic 55.0%
GAATGCCTCTGCTGCATCTC+TGG - chr1.1:4305380-4305399 MS.gene24450:intron 55.0%
!! GCTTTTGGGAGGGTCTGAGA+TGG - chr1.1:4305251-4305270 MS.gene24450:CDS 55.0%
CGCGTGGGGTTAACGCTCTT+TGG + chr1.1:4303572-4303591 None:intergenic 60.0%
TGCCACCAGAGATGCAGCAG+AGG + chr1.1:4305388-4305407 None:intergenic 60.0%
TGCCTCTGCTGCATCTCTGG+TGG - chr1.1:4305383-4305402 MS.gene24450:intron 60.0%
GGCAACAACACCCTCTCCCC+CGG - chr1.1:4306180-4306199 MS.gene24450:CDS 65.0%
TCACGTTGAGGGCGGCGCGT+GGG + chr1.1:4303587-4303606 None:intergenic 70.0%
TTCACGTTGAGGGCGGCGCG+TGG + chr1.1:4303588-4303607 None:intergenic 70.0%
CACGTTGAGGGCGGCGCGTG+GGG + chr1.1:4303586-4303605 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 4303563 4306246 4303563 ID=MS.gene24450
chr1.1 mRNA 4303563 4306246 4303563 ID=MS.gene24450.t1;Parent=MS.gene24450
chr1.1 exon 4305950 4306246 4305950 ID=MS.gene24450.t1.exon1;Parent=MS.gene24450.t1
chr1.1 CDS 4305950 4306246 4305950 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4305611 4305843 4305611 ID=MS.gene24450.t1.exon2;Parent=MS.gene24450.t1
chr1.1 CDS 4305611 4305843 4305611 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4305217 4305292 4305217 ID=MS.gene24450.t1.exon3;Parent=MS.gene24450.t1
chr1.1 CDS 4305217 4305292 4305217 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4305034 4305110 4305034 ID=MS.gene24450.t1.exon4;Parent=MS.gene24450.t1
chr1.1 CDS 4305034 4305110 4305034 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4304902 4304938 4304902 ID=MS.gene24450.t1.exon5;Parent=MS.gene24450.t1
chr1.1 CDS 4304902 4304938 4304902 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4304014 4304163 4304014 ID=MS.gene24450.t1.exon6;Parent=MS.gene24450.t1
chr1.1 CDS 4304014 4304163 4304014 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4303799 4303933 4303799 ID=MS.gene24450.t1.exon7;Parent=MS.gene24450.t1
chr1.1 CDS 4303799 4303933 4303799 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
chr1.1 exon 4303563 4303640 4303563 ID=MS.gene24450.t1.exon8;Parent=MS.gene24450.t1
chr1.1 CDS 4303563 4303640 4303563 ID=cds.MS.gene24450.t1;Parent=MS.gene24450.t1
Gene Sequence

>MS.gene24450

ATGGAACCAAAGAGCGTTAACCCCACGCGCCGCCCTCAACGTGAAGCAAAGAAAAAAGCCGTAGCTGCACTATGTGAACAACAACGCAAACGAAAACGCGTTCCTCTTGGTGAAATAACAAACGACGTAATCACCAACGCAAACGACGTCGTTTTACCAACGCAGAAAAAACGAAACGTTTCTACTTCATCAAACTCCGATGAAACTCCGTTACCGGAAAAGTTACAGAAATTCGAAGATCCTCAGTTATGTGAAACATATGTTTCGGATATATACGATTATCTTCGTAACATGGAGGTAATTTGATTTACTTAATTTAACTTCAAATTTCAATTAGGGTTTTGATTTTCCATAATTTGGGGCTTTTTGCTTAATTTAGGGTTTTTTTTTTCTTTGTTTTTAGGTTGATTCTAGTAAGAGGCCATTGTGTGATTATATACAGAAAGTTCAGAGGGATGTTAATGCGAGTATGAGGGGTGTTCTTGTTGATTGGTTGGTTGAAGTTGCTGAAGAATACAAGCTTGTTTCAGATACACTTTATTTTTCGGTTTCTTATATCGATCGGTTTTTGTCGTTGAATGATTTAACTAGACAGAAGTTGCAATTACTCGGTGTTGCTTCGATGTTGGTTGCTTCGTAAGTTTGTTTTTGTTTGTTGTTTTGATTTGTACTTTTGTATTGATGAATAAATACCTAATTTGGTCCCTCCCAATTTGTTTGCACCCAAATTAAAATAACACTCACATTGGTTTGTTGCATTCTATAATGGTGGTTAAATTAGTTCCTCTATTAATTTTGACCCATTTTAACAAAGAGATTACTCTTATGAAAATGTCAGGAATTAATTTGGTATATTGGACTATAAACAAATTGTGAGGAGCCAAGATGTGATTTTAGTCTGTATTGTTTATTTATTATTTATGCTTATTGATTTTTTATTTGTTTTGGATGTAGAAAATATGAAGAGATTAAGCCACCTGAGGTTGAGGACTTTTGTTACATTACGGACAATACATACTCCAAGGAAGAGGTGAATCTTGTAGTTTAAGTTTTTTGTTTGTGTTGTTGAATGTTTGTTTGGTTTTGGATCTTGCTGATTTTGGATGGTATTGTTTTGGTATTTTGTTTGATTAAAGGTTTTGACCATGGAGGCTGATATTTTGAAGTCATTGAAGTTTGAATTGGGTGGTCCTACAATAAAGACATTCCTAAGGCATGTGTGTTTTATTGATTATGTTTCTTTGTATGTTGAATGGAATTGTTTTTGAATGTTAGCTCACTTTTCATTTTTTCTTTTTCTGTTTGCAGAAGATTCATCACTAAAGTTGGTCTAGAGGGTGTTGATGTAAGTTATCTCATTCTTCAACCCATCTCATAGTAGTTATAGGTTATGACATGGATTCGGTGATTATGTGTGCTTATGTTCAACTTTTAGTAGTGTGGGACTTTTGGTTACTTTTGCTATTGTGTGTGGTTATGTCTTTATGACATTGATTATTCTCTCTTATCAAATAGTATCATCTAAGATAGGGAGATTGTGGAAATAAGAGATGTTGTATATAAATATTATCAAATTGTTGCATCTAAGATATGGAGATTGTTTGTGAAAATAAGAGATGTTGTATATAAATAGAAAGAGAATTAGAGTGGGTTTTGTCATGTCATTTGGAGAAATAGTTGAACTTTGAGCTTTTGGGAGGGTCTGAGATGGGAATTGAACCTTCTGGATAGTTCATTCAATACTAATTCATGAAAATCAAATTCCTATCTTGTTCTGCAGACTAACAAAACCCATGCAACCTTGCTAACTTTTTTAAGAATGCCTCTGCTGCATCTCTGGTGGCAAAGAGATGTTTCAACCCTAAATTTTTTGTCAAATGTGGCCAATCTAGCCACTTTCACAAAGAAAGTATCTTTGGTTTGTGCTATAGGAAGTTTCAAATCTAGTTATCCAGAAGTTTGAAATCAATTTTACTTGGATTATTATTTATTACTCAAAATGTTGCAGGCAAACCCATTTCCGTTTATGTGTGCCAAATCTGGAGACTTTGGTTTGTTTAATACTTTTATCTCTTTTGTGCAGGCTTCTGAGTTGCAGTTTGAGTTCCTTTGCTCTTACCTTGCTGAGCTCAGTTTGTTGGATTATAATTGTGTGAAGTTCTTGCCTTCTATGGTGGCTGCATCAGTTGTATTTCTTGCACGTTTTATGTTAAGCCCGAAGACTCATCCTTGGGTAATGTTTTAATTCATGTACAATCTATATTTGGTATTTGACTTTGACACAGTTACTAAAATATTTCCATTTGATTACAGAATTCAGCCATCTATGAATTCACCAGATATAAACCTGCAGACTTGAAGGAATGTGTTCTAAATATACATGACTTGTACCTTGGTAGGAAAGGCGGCTCTTTGCAAGCTGTGAGAGATAAATACAAACAGCATAAGGTGATATTCCTGCACGCTTATTGTCAATCCTTTTTTACCAAACAAACTTAATTTGTCCTACCTTGGCCCAAGCTTTTTAAACAATTGAGTTATTTATTTCATTACACTTGTCACATCTGATGCATTTATGCTGATTCCATTTGTTGTTTTGGTCACAGTTCAAATGCGTGGCAACAACACCCTCTCCCCCGGAGATATCTCTTTCTTTTTTTGAATTTAGAGGGGCTGATCCTTAG
Protein sequence

>MS.gene24450.t1

MEPKSVNPTRRPQREAKKKAVAALCEQQRKRKRVPLGEITNDVITNANDVVLPTQKKRNVSTSSNSDETPLPEKLQKFEDPQLCETYVSDIYDYLRNMEVDSSKRPLCDYIQKVQRDVNASMRGVLVDWLVEVAEEYKLVSDTLYFSVSYIDRFLSLNDLTRQKLQLLGVASMLVASKYEEIKPPEVEDFCYITDNTYSKEEVLTMEADILKSLKFELGGPTIKTFLRRFITKVGLEGVDASELQFEFLCSYLAELSLLDYNCVKFLPSMVAASVVFLARFMLSPKTHPWNSAIYEFTRYKPADLKECVLNIHDLYLGRKGGSLQAVRDKYKQHKFKCVATTPSPPEISLSFFEFRGADP