AlfalfaGEDB | MS.gene01024

M. sativa cultivar XinJiangDaYe / MS.gene01024

KEGG: K14009
GO: -
PFAM: PF17675.4 PF18035.4

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01024.t1	XP_013462866.1	99	192	2	0	1	192	31	222	1.00E-50	209.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01024.t1	A0A072V5J3	99.0	192	2	0	1	192	31	222	7.4e-51	209.9

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
MS.gene01024	MS.gene027264	PPI
MS.gene022965	MS.gene01024	PPI
MS.gene051700	MS.gene01024	PPI
MS.gene23697	MS.gene01024	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01024.t1	MTR_2g023880	98.958	192	2	0	1	192	31	222	1.90e-136	380
MS.gene01024.t1	MTR_4g049540	60.733	191	74	1	1	191	31	220	3.76e-82	243
MS.gene01024.t1	MTR_2g088060	49.738	191	95	1	1	191	31	220	9.17e-55	173
MS.gene01024.t1	MTR_7g056133	43.094	181	95	2	2	175	33	212	6.66e-41	138
MS.gene01024.t1	MTR_5g093360	55.882	68	29	1	2	69	32	98	4.05e-22	86.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01024.t1	AT5G42570	62.011	179	67	1	1	179	31	208	1.21e-77	231
MS.gene01024.t1	AT1G11905	60.000	180	71	1	1	180	31	209	1.52e-72	219
MS.gene01024.t1	AT1G11905	55.556	180	78	2	1	180	31	208	9.55e-61	189
MS.gene01024.t1	AT5G48660	39.560	182	102	2	2	176	33	213	1.56e-34	122
MS.gene01024.t1	AT3G07190	39.011	182	103	2	2	176	33	213	4.93e-33	118
MS.gene01024.t1	AT3G20450	52.475	101	47	1	3	102	37	137	2.57e-28	103
MS.gene01024.t1	AT5G48660	37.226	137	79	1	47	176	1	137	9.83e-20	81.6

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Find 43 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGAACAGTACCAGCAA+CGG	0.621936	2.4:-63561617	None:intergenic
AAGCCAGTGTAAGTGCTCTA+AGG	0.421670	2.4:+63563949	MS.gene01024:CDS
AAGCTGTTAAGAAACAAATG+CGG	0.709469	2.4:+63563793	MS.gene01024:CDS
ACAGACTGCATCATTACATA+AGG	0.453424	2.4:+63563742	MS.gene01024:CDS
ACATAAGGGAACTCCGGATT+CGG	0.458900	2.4:+63563757	MS.gene01024:CDS
ACGAGGTCGTGGACCTGTTA+TGG	0.464283	2.4:+63561588	MS.gene01024:CDS
ACTTCAGTCAAAAGATAAAG+AGG	0.526615	2.4:+63563914	MS.gene01024:CDS
AGCTGTTAAGAAACAAATGC+GGG	0.550293	2.4:+63563794	MS.gene01024:CDS
ATCATTACATAAGGGAACTC+CGG	0.575747	2.4:+63563751	MS.gene01024:CDS
ATGATGAAGATTCAACAACG+TGG	0.740230	2.4:+63561664	MS.gene01024:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCTGTTAAGAAACAAATG+CGG	+	chr2.4:63563793-63563812	MS.gene01024:CDS	30.0%
AAGTTGAAAAATGTGAGCTT+TGG	+	chr2.4:63563287-63563306	MS.gene01024:intron	30.0%
ACAATGATTCAGCATTCATA+AGG	+	chr2.4:63562956-63562975	MS.gene01024:intron	30.0%
ACTTCAGTCAAAAGATAAAG+AGG	+	chr2.4:63563914-63563933	MS.gene01024:CDS	30.0%
AGATCTTCTCAATTTCCAAA+AGG	-	chr2.4:63562194-63562213	None:intergenic	30.0%
ATTCTTGGTCAATTATTTGC+AGG	+	chr2.4:63563690-63563709	MS.gene01024:intron	30.0%
CAGTATTGTTTATGTTGGAT+TGG	-	chr2.4:63563594-63563613	None:intergenic	30.0%
CTATCATAAGTGCAAGAAAA+AGG	-	chr2.4:63563722-63563741	None:intergenic	30.0%
CTCAACAGTATTGTTTATGT+TGG	-	chr2.4:63563599-63563618	None:intergenic	30.0%
GAATAACCCAACAAAGAATT+AGG	-	chr2.4:63563177-63563196	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	63561568	63564094	63561568	ID=MS.gene01024
chr2.4	mRNA	63561568	63564094	63561568	ID=MS.gene01024.t1;Parent=MS.gene01024
chr2.4	exon	63561568	63561763	63561568	ID=MS.gene01024.t1.exon1;Parent=MS.gene01024.t1
chr2.4	CDS	63561568	63561763	63561568	ID=cds.MS.gene01024.t1;Parent=MS.gene01024.t1
chr2.4	exon	63563712	63564094	63563712	ID=MS.gene01024.t1.exon2;Parent=MS.gene01024.t1
chr2.4	CDS	63563712	63564094	63563712	ID=cds.MS.gene01024.t1;Parent=MS.gene01024.t1

Gene Sequence

>MS.gene01024

ATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAACATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTATGTTTTTTTTAAATGGGTTGTTGAAAAGTTTTGATTTTTGTTTATTTGCTTGATGAATGAATGAATTTTGTATGAGGAATTGAATTTGATTTATGTTTATGGATTTGGATTTAATGTGATTGCTCTGTTATGTGTAATTGTTTGCATTATTAATTTATTGAGTTTTTAGCATTATTATTTTTGTAAATATGATCAAATAGGTACATTTTTGTAATGTGTATAATTTTGCTTATAATTAAGACTGGACGGAGTATCTGTCAAGCACCGGATCCGATACGGAAACTAGATATGACACTGACACAGACACGTTGGAACAAAGAACGTTTTGTATGAGTTGTGAGATACATTTGTAAGAGTGTTGTACGGATGTAATACACCGGTGTGTGTTGGTCACGGAGATATGAATATATGATCAATCACAAAACCTTTTGGAAATTGAGAAGATCTTCTCGAATTCATGAACCCATTTTCTGATCTTTCGGAGTCATGTTCCCTTTTCCTTTCATTAATGTAGTCAATCTTGCATTGTGATGAATATGTTGTCTCTGTTGTTGCTGATGCAATTGATGAGTGATGATCTCTAGTGATCTAAAAGATTTGTTTGTTTTGCTGCATCTGATGCAGCATGGCTGCCTTCAATTCTCGAATGGTTCTTGAGTTTACTGTGCCTCTGGCTCTTTCCCCAGTCCGAGAGGCTTTACCCTGTCAAACGGGCTCCTCCTGTCCTCTGTTGTCAAAAGCGGCCGCACCGGCCGCTATAGCGGAGCATAGCAGAAAAATCAGGACCACTGCGCCAGGCCGCGTCGGCGGGTATCAGAAGTGGAAAGCGGCAAAGCGGACCTATCAATAGTAAAACAATAACTTTTACTAGTTGTATATAATTGGTATTAGCGATTTTCAATTTTGCTATCCCTTTTATTTCATATTATGAATACTTCATGAAGTTTTGAGTAATGTTTAAGGTATTTTAGTATTTCTTTTGAATAGTATGGTATATAGTATTAAACTTATTAGTGGCTGCACCACCGAAATAGCGCCCGCTATGCGCCACACCGTATTTCTGCATTGACAACAGAGCTCCTGTCTAGGTTCAGCTTCTAGACCTCTCTCAGCTCCCCTGTTGGCACCTCTTCCAGTGCCTATACCTCGTGAACGTGTATTTCGAACCGTGTTTTCCTGAAAAAGAATCACAATGATTCAGCATTCATAAGGCATAAGGATCATATAAACCATATGCATATATCTTATGATTGATTTAGTTAAAGAGAAAGAGCCTTCACCCTTGGGTAAACCGGTCACTCTTGGTATTTTAGCCTCCTAATGGGCTCATATGATTCAGACGCTCAACACTCATATAACTGAAGCTTCTAACTTGTAACTTGTGAGTGAAGCCTCGTTGGTTGTTTGCCTAATTCTTTGTTGGGTTATTCTGTAAATATTTGTGTTTCTTTTTGCTACCGATTTTTTCAATGGTTTAGAATGAATGTTGAGGCAACTTACATGAGATATGTGATCTTCTAAAGTTGAAAAATGTGAGCTTTGGAGAATGTTCTACTTTCTTGTGGTATTGTTTATTGATGAGTTATTGGTGGCATTAGCAGGGAGATTGAAATCAGATGTACTATATGATTTGCAATTTTTAGGATTCTCTCTCTCACAACTAGAAGCCATCTAGTATGTTGTTAAACTTTACAACTCAGATTGTATGTAATTTCCTAAGTTCAACATATTTCCAAATTTTTCAAAACATTGCTGTGTTCTTTGATTTTTAATAAGACCGGTCACATCTTAAAACAACATAGCTGAAGAGGTAAAGTTACAATTCCAATCCAACATAAACAATACTGTTGAGTTTCTGAACTGATTGTTCCTTAATATTTAGACTTATTAAAAGATATCCTCTGTGGCTTGCGAGCATTATTCATTCTTGGTCAATTATTTGCAGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCTGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA

Protein sequence

>MS.gene01024.t1

MALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEHGASVNPTDQVLMAKHLLEATLMGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM