Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23697.t1 | XP_013448596.1 | 99.1 | 218 | 2 | 0 | 1 | 218 | 1 | 218 | 5.80E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23697.t1 | A0A072U0E6 | 99.1 | 218 | 2 | 0 | 1 | 218 | 1 | 218 | 4.2e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene23697 | 0.855208 | 7.36E-62 | -1.69E-46 |
| MS.gene049178 | MS.gene23697 | 0.8329 | 7.26E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene23697 | 0.811254 | 7.68E-51 | -1.69E-46 |
| MS.gene049271 | MS.gene23697 | 0.80547 | 1.31E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene23697 | 0.840926 | 6.48E-58 | -1.69E-46 |
| MS.gene049368 | MS.gene23697 | 0.803909 | 2.78E-49 | -1.69E-46 |
| MS.gene049441 | MS.gene23697 | 0.804335 | 2.27E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene23697 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene049647 | MS.gene23697 | 0.811916 | 5.51E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene23697 | 0.823056 | 1.70E-53 | -1.69E-46 |
| MS.gene049807 | MS.gene23697 | 0.801492 | 8.78E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene23697 | 0.831577 | 1.54E-55 | -1.69E-46 |
| MS.gene049824 | MS.gene23697 | 0.85412 | 1.52E-61 | -1.69E-46 |
| MS.gene049825 | MS.gene23697 | 0.853443 | 2.38E-61 | -1.69E-46 |
| MS.gene049850 | MS.gene23697 | 0.829612 | 4.67E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene23697 | 0.82703 | 1.96E-54 | -1.69E-46 |
| MS.gene049887 | MS.gene23697 | 0.818744 | 1.67E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene23697 | 0.81631 | 5.91E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene23697 | 0.805898 | 1.07E-49 | -1.69E-46 |
| MS.gene050439 | MS.gene23697 | 0.802305 | 5.98E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene23697 | 0.82044 | 6.85E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene23697 | 0.805493 | 1.30E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene23697 | 0.801652 | 8.14E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene23697 | 0.865225 | 6.89E-65 | -1.69E-46 |
| MS.gene051279 | MS.gene23697 | 0.812415 | 4.29E-51 | -1.69E-46 |
| MS.gene051388 | MS.gene23697 | 0.816555 | 5.21E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene23697 | 0.805194 | 1.50E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene23697 | 0.944686 | 1.23E-103 | -1.69E-46 |
| MS.gene051705 | MS.gene23697 | 0.826425 | 2.73E-54 | -1.69E-46 |
| MS.gene051719 | MS.gene23697 | 0.852788 | 3.67E-61 | -1.69E-46 |
| MS.gene051720 | MS.gene23697 | 0.869643 | 2.65E-66 | -1.69E-46 |
| MS.gene051744 | MS.gene23697 | 0.828109 | 1.08E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene23697 | 0.836607 | 8.48E-57 | -1.69E-46 |
| MS.gene051975 | MS.gene23697 | 0.805343 | 1.40E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene23697 | 0.852727 | 3.82E-61 | -1.69E-46 |
| MS.gene052463 | MS.gene23697 | 0.806325 | 8.69E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene23697 | 0.824431 | 8.10E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene23697 | 0.823441 | 1.38E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene23697 | 0.846095 | 2.69E-59 | -1.69E-46 |
| MS.gene052584 | MS.gene23697 | 0.855456 | 6.23E-62 | -1.69E-46 |
| MS.gene052665 | MS.gene23697 | 0.815139 | 1.08E-51 | -1.69E-46 |
| MS.gene052673 | MS.gene23697 | -0.80983 | 1.56E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene23697 | 0.813574 | 2.39E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene23697 | 0.805884 | 1.08E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene23697 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene053079 | MS.gene23697 | 0.804673 | 1.93E-49 | -1.69E-46 |
| MS.gene053122 | MS.gene23697 | 0.860722 | 1.69E-63 | -1.69E-46 |
| MS.gene053144 | MS.gene23697 | 0.827816 | 1.27E-54 | -1.69E-46 |
| MS.gene053221 | MS.gene23697 | 0.805534 | 1.27E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene23697 | 0.877509 | 5.93E-69 | -1.69E-46 |
| MS.gene053581 | MS.gene23697 | 0.818275 | 2.13E-52 | -1.69E-46 |
| MS.gene053599 | MS.gene23697 | 0.838106 | 3.50E-57 | -1.69E-46 |
| MS.gene05364 | MS.gene23697 | 0.829618 | 4.65E-55 | -1.69E-46 |
| MS.gene05365 | MS.gene23697 | 0.812826 | 3.49E-51 | -1.69E-46 |
| MS.gene054139 | MS.gene23697 | 0.825141 | 5.51E-54 | -1.69E-46 |
| MS.gene054576 | MS.gene23697 | 0.812309 | 4.52E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene23697 | 0.83234 | 1.00E-55 | -1.69E-46 |
| MS.gene05458 | MS.gene23697 | 0.809382 | 1.95E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene23697 | 0.811764 | 5.95E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene23697 | 0.821511 | 3.88E-53 | -1.69E-46 |
| MS.gene054967 | MS.gene23697 | 0.82897 | 6.69E-55 | -1.69E-46 |
| MS.gene054991 | MS.gene23697 | 0.821309 | 4.32E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene23697 | 0.812526 | 4.06E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene23697 | 0.857665 | 1.40E-62 | -1.69E-46 |
| MS.gene055466 | MS.gene23697 | 0.859981 | 2.84E-63 | -1.69E-46 |
| MS.gene055468 | MS.gene23697 | 0.867897 | 9.75E-66 | -1.69E-46 |
| MS.gene055469 | MS.gene23697 | 0.84953 | 3.05E-60 | -1.69E-46 |
| MS.gene055512 | MS.gene23697 | 0.817301 | 3.54E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene23697 | 0.840493 | 8.41E-58 | -1.69E-46 |
| MS.gene055537 | MS.gene23697 | 0.832225 | 1.07E-55 | -1.69E-46 |
| MS.gene055555 | MS.gene23697 | 0.80173 | 7.84E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene23697 | -0.864938 | 8.48E-65 | -1.69E-46 |
| MS.gene055778 | MS.gene23697 | 0.833029 | 6.74E-56 | -1.69E-46 |
| MS.gene055808 | MS.gene23697 | 0.814591 | 1.42E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene23697 | 0.810609 | 1.06E-50 | -1.69E-46 |
| MS.gene056005 | MS.gene23697 | 0.811884 | 5.60E-51 | -1.69E-46 |
| MS.gene056043 | MS.gene23697 | -0.858052 | 1.07E-62 | -1.69E-46 |
| MS.gene05604 | MS.gene23697 | 0.806076 | 9.81E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene23697 | 0.817967 | 2.51E-52 | -1.69E-46 |
| MS.gene056091 | MS.gene23697 | 0.829996 | 3.76E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene23697 | 0.856256 | 3.64E-62 | -1.69E-46 |
| MS.gene056308 | MS.gene23697 | 0.849956 | 2.32E-60 | -1.69E-46 |
| MS.gene056366 | MS.gene23697 | 0.819415 | 1.18E-52 | -1.69E-46 |
| MS.gene056390 | MS.gene23697 | 0.802492 | 5.47E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene23697 | 0.832991 | 6.89E-56 | -1.69E-46 |
| MS.gene056595 | MS.gene23697 | 0.820356 | 7.16E-53 | -1.69E-46 |
| MS.gene05659 | MS.gene23697 | 0.80253 | 5.37E-49 | -1.69E-46 |
| MS.gene056605 | MS.gene23697 | 0.814544 | 1.46E-51 | -1.69E-46 |
| MS.gene05689 | MS.gene23697 | 0.811465 | 6.91E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene23697 | 0.813217 | 2.86E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene23697 | 0.805782 | 1.13E-49 | -1.69E-46 |
| MS.gene057360 | MS.gene23697 | 0.827513 | 1.50E-54 | -1.69E-46 |
| MS.gene057693 | MS.gene23697 | 0.800683 | 1.28E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene23697 | 0.831648 | 1.48E-55 | -1.69E-46 |
| MS.gene058330 | MS.gene23697 | 0.801348 | 9.39E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene23697 | 0.822185 | 2.71E-53 | -1.69E-46 |
| MS.gene058636 | MS.gene23697 | 0.822907 | 1.84E-53 | -1.69E-46 |
| MS.gene058668 | MS.gene23697 | 0.864633 | 1.06E-64 | -1.69E-46 |
| MS.gene058852 | MS.gene23697 | 0.843427 | 1.41E-58 | -1.69E-46 |
| MS.gene059084 | MS.gene23697 | 0.848708 | 5.16E-60 | -1.69E-46 |
| MS.gene059170 | MS.gene23697 | 0.806157 | 9.43E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene23697 | 0.81176 | 5.96E-51 | -1.69E-46 |
| MS.gene059375 | MS.gene23697 | 0.808894 | 2.47E-50 | -1.69E-46 |
| MS.gene05950 | MS.gene23697 | 0.830541 | 2.77E-55 | -1.69E-46 |
| MS.gene05953 | MS.gene23697 | 0.808523 | 2.97E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene23697 | 0.838232 | 3.25E-57 | -1.69E-46 |
| MS.gene059570 | MS.gene23697 | 0.80616 | 9.41E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene23697 | 0.839643 | 1.40E-57 | -1.69E-46 |
| MS.gene059725 | MS.gene23697 | 0.823023 | 1.73E-53 | -1.69E-46 |
| MS.gene059842 | MS.gene23697 | 0.808944 | 2.42E-50 | -1.69E-46 |
| MS.gene059935 | MS.gene23697 | 0.810468 | 1.14E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene23697 | 0.829012 | 6.53E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene23697 | 0.843802 | 1.12E-58 | -1.69E-46 |
| MS.gene060429 | MS.gene23697 | 0.865772 | 4.63E-65 | -1.69E-46 |
| MS.gene060587 | MS.gene23697 | 0.847915 | 8.55E-60 | -1.69E-46 |
| MS.gene060637 | MS.gene23697 | 0.820903 | 5.36E-53 | -1.69E-46 |
| MS.gene060763 | MS.gene23697 | 0.80461 | 1.99E-49 | -1.69E-46 |
| MS.gene060907 | MS.gene23697 | 0.814363 | 1.60E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23697 | MS.gene00790 | PPI |
| MS.gene27209 | MS.gene23697 | PPI |
| MS.gene00793 | MS.gene23697 | PPI |
| MS.gene02358 | MS.gene23697 | PPI |
| MS.gene00792 | MS.gene23697 | PPI |
| MS.gene23697 | MS.gene01024 | PPI |
| MS.gene23697 | MS.gene002988 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23697.t1 | MTR_7g056133 | 99.541 | 218 | 1 | 0 | 1 | 218 | 1 | 218 | 5.23e-157 | 433 |
| MS.gene23697.t1 | MTR_4g049540 | 41.981 | 212 | 115 | 2 | 1 | 212 | 1 | 204 | 3.16e-52 | 168 |
| MS.gene23697.t1 | MTR_2g023880 | 44.131 | 213 | 110 | 3 | 1 | 212 | 1 | 205 | 1.73e-51 | 166 |
| MS.gene23697.t1 | MTR_2g088060 | 39.336 | 211 | 119 | 3 | 1 | 210 | 1 | 203 | 1.46e-35 | 125 |
| MS.gene23697.t1 | MTR_5g093360 | 38.542 | 96 | 58 | 1 | 1 | 96 | 1 | 95 | 1.93e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23697.t1 | AT5G48660 | 75.799 | 219 | 52 | 1 | 1 | 218 | 1 | 219 | 1.86e-94 | 275 |
| MS.gene23697.t1 | AT3G07190 | 74.057 | 212 | 55 | 0 | 1 | 212 | 1 | 212 | 1.22e-92 | 271 |
| MS.gene23697.t1 | AT5G42570 | 44.340 | 212 | 110 | 2 | 1 | 212 | 1 | 204 | 7.30e-55 | 174 |
| MS.gene23697.t1 | AT5G48660 | 74.126 | 143 | 36 | 1 | 77 | 218 | 1 | 143 | 9.08e-47 | 151 |
| MS.gene23697.t1 | AT1G11905 | 36.321 | 212 | 127 | 2 | 1 | 212 | 1 | 204 | 4.23e-40 | 137 |
| MS.gene23697.t1 | AT1G11905 | 33.019 | 212 | 133 | 3 | 1 | 212 | 1 | 203 | 8.02e-30 | 110 |
| MS.gene23697.t1 | AT3G20450 | 39.516 | 124 | 71 | 3 | 1 | 122 | 5 | 126 | 3.52e-21 | 85.9 |
Find 35 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAAAAGCTTATTAATTTA+AGG | 0.061758 | 7.1:+45912876 | MS.gene23697:CDS |
| CTGCTTGAGCCTGAAGATTT+TGG | 0.137576 | 7.1:-45913159 | None:intergenic |
| TCCTTAAATTAATAAGCTTT+TGG | 0.250509 | 7.1:-45912877 | None:intergenic |
| CAAGCTTAGCACCTTTGTTC+TGG | 0.274823 | 7.1:-45901352 | None:intergenic |
| TTCATCACAAGATCCCTTAA+TGG | 0.289583 | 7.1:-45901224 | None:intergenic |
| TGGTAAAGATAGGTCCATTA+AGG | 0.319406 | 7.1:+45901210 | MS.gene23697:CDS |
| AACTCTCTTGTCTTTCTCTT+CGG | 0.340250 | 7.1:-45913053 | None:intergenic |
| TCTAACAGAAGGTTTGAAAA+AGG | 0.345623 | 7.1:+45913018 | MS.gene23697:CDS |
| GGATCTTGTGATGAAGAGTT+TGG | 0.371144 | 7.1:+45901232 | MS.gene23697:CDS |
| GATTATTGCTGAAGGTGTTA+TGG | 0.376307 | 7.1:+45901175 | MS.gene23697:CDS |
| TTGCTTGAAGCTTATCTCCT+TGG | 0.381860 | 7.1:+45901416 | MS.gene23697:CDS |
| GGAAGACAACCAAAATCTTC+AGG | 0.401724 | 7.1:+45913150 | MS.gene23697:CDS |
| TTTCTTATGATGGTAAAGAT+AGG | 0.402027 | 7.1:+45901200 | MS.gene23697:CDS |
| CACCTATGGATCAAGTTCTC+TGG | 0.452114 | 7.1:+45901384 | MS.gene23697:CDS |
| CAAAATCTTCAGGCTCAAGC+AGG | 0.486517 | 7.1:+45913160 | MS.gene23697:CDS |
| GCTTGGTACTATGTCACCTA+TGG | 0.487529 | 7.1:+45901370 | MS.gene23697:CDS |
| AAATGTCGAGCTCAAAGAAA+AGG | 0.491750 | 7.1:+45912946 | MS.gene23697:CDS |
| GCTTGAATATGACAGACTCT+TGG | 0.508233 | 7.1:+45913129 | MS.gene23697:CDS |
| GGTAAAGATAGGTCCATTAA+GGG | 0.516157 | 7.1:+45901211 | MS.gene23697:CDS |
| GAGTTTGGATCAACTCAAGA+TGG | 0.522875 | 7.1:+45901247 | MS.gene23697:CDS |
| GAACTTTCAACTCTAACAGA+AGG | 0.533214 | 7.1:+45913007 | MS.gene23697:CDS |
| CAGAACAAAGGTGCTAAGCT+TGG | 0.534297 | 7.1:+45901353 | MS.gene23697:CDS |
| AAAGGAGGAGAAAGCTTCCA+AGG | 0.549039 | 7.1:+45912964 | MS.gene23697:CDS |
| GGGAGCTTCAAAAGAAGAAG+TGG | 0.560241 | 7.1:+45912907 | MS.gene23697:CDS |
| TATTAATTTAAGGAGTAACA+TGG | 0.564092 | 7.1:+45912886 | MS.gene23697:CDS |
| CTCCAGAGAACTTGATCCAT+AGG | 0.565099 | 7.1:-45901386 | None:intergenic |
| GAAAGACAAGAGAGTTGAGA+CGG | 0.586053 | 7.1:+45913060 | MS.gene23697:CDS |
| ATTAATTTAAGGAGTAACAT+GGG | 0.596773 | 7.1:+45912887 | MS.gene23697:CDS |
| GCAGGGAGGCATAAGAGTTG+AGG | 0.597943 | 7.1:+45913178 | MS.gene23697:CDS |
| AGTTTGGATCAACTCAAGAT+GGG | 0.605908 | 7.1:+45901248 | MS.gene23697:CDS |
| AAAATCTTCAGGCTCAAGCA+GGG | 0.625078 | 7.1:+45913161 | MS.gene23697:CDS |
| TGTCGAGCTCAAAGAAAAGG+AGG | 0.645126 | 7.1:+45912949 | MS.gene23697:CDS |
| CTTGTTAAGATCCAGAACAA+AGG | 0.651048 | 7.1:+45901341 | MS.gene23697:CDS |
| TTCGATCAATTAGAAACCCG+AGG | 0.671263 | 7.1:-45912841 | None:intergenic |
| ATCTTCAGGCTCAAGCAGGG+AGG | 0.695143 | 7.1:+45913164 | MS.gene23697:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 45901152 | 45913198 | 45901152 | ID=MS.gene23697 |
| chr7.1 | mRNA | 45901152 | 45913198 | 45901152 | ID=MS.gene23697.t1;Parent=MS.gene23697 |
| chr7.1 | exon | 45901152 | 45901437 | 45901152 | ID=MS.gene23697.t1.exon1;Parent=MS.gene23697.t1 |
| chr7.1 | CDS | 45901152 | 45901437 | 45901152 | ID=cds.MS.gene23697.t1;Parent=MS.gene23697.t1 |
| chr7.1 | exon | 45912828 | 45913198 | 45912828 | ID=MS.gene23697.t1.exon2;Parent=MS.gene23697.t1 |
| chr7.1 | CDS | 45912828 | 45913198 | 45912828 | ID=cds.MS.gene23697.t1;Parent=MS.gene23697.t1 |
| Gene Sequence |
| Protein sequence |
