AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90649


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90649.t1 MTR_2g084985 99.099 111 1 0 1 111 1 111 6.00e-75 217
MS.gene90649.t1 MTR_4g124920 41.584 101 55 2 8 105 4 103 1.40e-21 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90649.t1 AT5G22580 65.657 99 34 0 7 105 6 104 5.30e-42 134
MS.gene90649.t1 AT3G17210 42.000 100 54 2 8 104 9 107 3.39e-22 84.0
MS.gene90649.t1 AT3G17210 42.000 100 54 2 8 104 9 107 3.39e-22 84.0

Find 22 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATGCTGCTTCCTTGAC+TGG 0.289662 2.3:-12875089 None:intergenic
TACAAAAGGGATGGAGAAAT+TGG 0.322091 2.3:+12874200 MS.gene90649:CDS
CTTCCTTGACTGGCTTGACA+AGG 0.415501 2.3:-12875079 None:intergenic
ATCTTGTCAAATCCTTTGAA+TGG 0.422994 2.3:+12874235 MS.gene90649:CDS
ACTGGCTTGACAAGGGTTGA+AGG 0.449844 2.3:-12875071 None:intergenic
CTTGTTGAATGTCATCAAGA+AGG 0.457412 2.3:-12874952 None:intergenic
GTGATTGTTAAGTTCAAAGA+AGG 0.458164 2.3:+12874159 MS.gene90649:CDS
GTAGTGGAAGAGCTTACAAA+AGG 0.480357 2.3:+12874186 MS.gene90649:CDS
GTTCAAAGAAGGTGTAGTAG+TGG 0.481023 2.3:+12874170 MS.gene90649:CDS
TCAGAGAACTCAACATGACT+TGG 0.494285 2.3:-12875005 None:intergenic
AACCTTGTCTCAACACATCT+AGG 0.520013 2.3:-12874920 None:intergenic
CTGGCTTGACAAGGGTTGAA+GGG 0.536665 2.3:-12875070 None:intergenic
TTCCTTGACTGGCTTGACAA+GGG 0.547981 2.3:-12875078 None:intergenic
AGAACTCAACATGACTTGGA+TGG 0.554383 2.3:-12875001 None:intergenic
GTATGTCTTATACAGGGGAC+AGG 0.555403 2.3:+12874887 MS.gene90649:intron
TTCACAAAGAATGGTAGTCA+TGG 0.559784 2.3:+12874119 None:intergenic
GGATAAAACATACCATTCAA+AGG 0.573224 2.3:-12874247 None:intergenic
AGCCTAGATGTGTTGAGACA+AGG 0.579475 2.3:+12874918 MS.gene90649:CDS
AACCCTTGTCAAGCCAGTCA+AGG 0.583977 2.3:+12875076 MS.gene90649:CDS
TAGTGGAAGAGCTTACAAAA+GGG 0.588758 2.3:+12874187 MS.gene90649:CDS
GGAAGAGCTTACAAAAGGGA+TGG 0.589362 2.3:+12874191 MS.gene90649:CDS
ACAAAGAATGGTAGTCATGG+AGG 0.636423 2.3:+12874122 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTATCAGTATCTATA+AGG - chr2.3:12874365-12874384 None:intergenic 20.0%
!! AACAAAGACAGTAATTTAAA+GGG + chr2.3:12874448-12874467 MS.gene90649:intron 20.0%
!! ACTGAGATTTATAGTTAAAT+AGG + chr2.3:12874564-12874583 MS.gene90649:intron 20.0%
!! ATGTGATGATTATGATTATT+TGG + chr2.3:12874800-12874819 MS.gene90649:intron 20.0%
!! TGCATAATATAAAAGGTAAA+TGG - chr2.3:12874271-12874290 None:intergenic 20.0%
!!! ACATCAAAAACTTTGATTTT+GGG + chr2.3:12874618-12874637 MS.gene90649:intron 20.0%
! AACAGAAACAGAAATAACAA+GGG + chr2.3:12874415-12874434 MS.gene90649:intron 25.0%
! AATCAAGTATGTCTTATACA+GGG + chr2.3:12874881-12874900 MS.gene90649:intron 25.0%
! ATGATATGATAGTACTAAGT+AGG - chr2.3:12874852-12874871 None:intergenic 25.0%
! CTATAAGGTTAATAAACTGA+AGG - chr2.3:12874350-12874369 None:intergenic 25.0%
! GAACAAAGACAGTAATTTAA+AGG + chr2.3:12874447-12874466 MS.gene90649:intron 25.0%
! TAACAGAAACAGAAATAACA+AGG + chr2.3:12874414-12874433 MS.gene90649:intron 25.0%
! TAATCAAGTATGTCTTATAC+AGG + chr2.3:12874880-12874899 MS.gene90649:intron 25.0%
! TGATATGATAGTACTAAGTA+GGG - chr2.3:12874851-12874870 None:intergenic 25.0%
!! TACCTTTTATATTATGCAAG+AGG + chr2.3:12874273-12874292 MS.gene90649:intron 25.0%
!!! AATATCAAGGTTTTAGATTG+AGG + chr2.3:12874516-12874535 MS.gene90649:intron 25.0%
!!! GACATCAAAAACTTTGATTT+TGG + chr2.3:12874617-12874636 MS.gene90649:intron 25.0%
ATCAAGTATGTCTTATACAG+GGG + chr2.3:12874882-12874901 MS.gene90649:intron 30.0%
ATCTTGTCAAATCCTTTGAA+TGG + chr2.3:12874235-12874254 MS.gene90649:CDS 30.0%
GGATAAAACATACCATTCAA+AGG - chr2.3:12874250-12874269 None:intergenic 30.0%
GTGATTGTTAAGTTCAAAGA+AGG + chr2.3:12874159-12874178 MS.gene90649:CDS 30.0%
TCTCATGTGCTTAAATATCA+AGG + chr2.3:12874503-12874522 MS.gene90649:intron 30.0%
TCTGTTATCGAATATATCAG+TGG - chr2.3:12874401-12874420 None:intergenic 30.0%
TGCCTCTTGCATAATATAAA+AGG - chr2.3:12874278-12874297 None:intergenic 30.0%
CTTGTTGAATGTCATCAAGA+AGG - chr2.3:12874955-12874974 None:intergenic 35.0%
GAACTATCTTTGTCAAGTGT+TGG - chr2.3:12874485-12874504 None:intergenic 35.0%
TACAAAAGGGATGGAGAAAT+TGG + chr2.3:12874200-12874219 MS.gene90649:CDS 35.0%
TAGTGGAAGAGCTTACAAAA+GGG + chr2.3:12874187-12874206 MS.gene90649:CDS 35.0%
! GTATACGATTAGTTGTGACT+TGG - chr2.3:12874736-12874755 None:intergenic 35.0%
! TTGATTTTGGGCTTACATCA+CGG + chr2.3:12874630-12874649 MS.gene90649:intron 35.0%
!! ATCTTTGTCAAGTGTTGGTT+GGG - chr2.3:12874480-12874499 None:intergenic 35.0%
!! TATCTTTGTCAAGTGTTGGT+TGG - chr2.3:12874481-12874500 None:intergenic 35.0%
!!! AAGGTTTTAGATTGAGGTTG+CGG + chr2.3:12874522-12874541 MS.gene90649:intron 35.0%
AACCTTGTCTCAACACATCT+AGG - chr2.3:12874923-12874942 None:intergenic 40.0%
TCAGAGAACTCAACATGACT+TGG - chr2.3:12875008-12875027 None:intergenic 40.0%
! AGAACTCAACATGACTTGGA+TGG - chr2.3:12875004-12875023 None:intergenic 40.0%
! AGTTTTTGATGTCTTCGCGT+TGG - chr2.3:12874610-12874629 None:intergenic 40.0%
! GTAGTGGAAGAGCTTACAAA+AGG + chr2.3:12874186-12874205 MS.gene90649:CDS 40.0%
! GTTCAAAGAAGGTGTAGTAG+TGG + chr2.3:12874170-12874189 MS.gene90649:CDS 40.0%
!! GATTAGTTGTGACTTGGTGT+CGG - chr2.3:12874730-12874749 None:intergenic 40.0%
!! TCTTTGTCAAGTGTTGGTTG+GGG - chr2.3:12874479-12874498 None:intergenic 40.0%
AGCCTAGATGTGTTGAGACA+AGG + chr2.3:12874918-12874937 MS.gene90649:CDS 45.0%
CGGTCGTATACTCGTAGTTA+TGG + chr2.3:12874650-12874669 MS.gene90649:intron 45.0%
GGAAGAGCTTACAAAAGGGA+TGG + chr2.3:12874191-12874210 MS.gene90649:CDS 45.0%
GTATGTCTTATACAGGGGAC+AGG + chr2.3:12874887-12874906 MS.gene90649:intron 45.0%
!! ATGTCTTCGCGTTGGTAATG+TGG - chr2.3:12874602-12874621 None:intergenic 45.0%
!! TTCCTTGACTGGCTTGACAA+GGG - chr2.3:12875081-12875100 None:intergenic 45.0%
AACCCTTGTCAAGCCAGTCA+AGG + chr2.3:12875076-12875095 MS.gene90649:CDS 50.0%
TGAGGTTGCGGTCATGTTCA+CGG + chr2.3:12874534-12874553 MS.gene90649:intron 50.0%
! CTGGCTTGACAAGGGTTGAA+GGG - chr2.3:12875073-12875092 None:intergenic 50.0%
! TGGTAATGTGGCTGCAATCG+AGG - chr2.3:12874590-12874609 None:intergenic 50.0%
!! ACTGGCTTGACAAGGGTTGA+AGG - chr2.3:12875074-12875093 None:intergenic 50.0%
!! CTTCCTTGACTGGCTTGACA+AGG - chr2.3:12875082-12875101 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12874129 12875109 12874129 ID=MS.gene90649
chr2.3 mRNA 12874129 12875109 12874129 ID=MS.gene90649.t1;Parent=MS.gene90649
chr2.3 exon 12874129 12874256 12874129 ID=MS.gene90649.t1.exon1;Parent=MS.gene90649.t1
chr2.3 CDS 12874129 12874256 12874129 ID=cds.MS.gene90649.t1;Parent=MS.gene90649.t1
chr2.3 exon 12874902 12875109 12874902 ID=MS.gene90649.t1.exon2;Parent=MS.gene90649.t1
chr2.3 CDS 12874902 12875109 12874902 ID=cds.MS.gene90649.t1;Parent=MS.gene90649.t1
Gene Sequence

>MS.gene90649

ATGGTAGTCATGGAGGAATTCAAGCACTTTGTGATTGTTAAGTTCAAAGAAGGTGTAGTAGTGGAAGAGCTTACAAAAGGGATGGAGAAATTGGTCTCAGAGATTGATCTTGTCAAATCCTTTGAATGGTATGTTTTATCCATTTACCTTTTATATTATGCAAGAGGCAGTATTATACTTCAGTAAATATAAGAACAAGTAGTTAAACATTCTTAAGTCCTTCAGTTTATTAACCTTATAGATACTGATAGTTTTTTTATCATGTTACACCACTGATATATTCGATAACAGAAACAGAAATAACAAGGGTTCAAAGTAGAACAAAGACAGTAATTTAAAGGGAAATTCCCCAACCAACACTTGACAAAGATAGTTCTCATGTGCTTAAATATCAAGGTTTTAGATTGAGGTTGCGGTCATGTTCACGGTGATTTTACTGAGATTTATAGTTAAATAGGCCTCGATTGCAGCCACATTACCAACGCGAAGACATCAAAAACTTTGATTTTGGGCTTACATCACGGTCGTATACTCGTAGTTATGGATTTATTTTTCAAGAGTTTAAGTTTGATATATCGTCAATATAAATAATTGTATACCGACACCAAGTCACAACTAATCGTATACATATGACTTCAAAATTAGTAGTTATAAGTAATCAGTTTTTAACGATGTGATGATTATGATTATTTGGCATTGTAAAAAATCTTTGCACAAAACCCTACTTAGTACTATCATATCATAAGCTTTTTAATCAAGTATGTCTTATACAGGGGACAGGATATTGAAAGCCTAGATGTGTTGAGACAAGGTTTCACTCATGCCTTCTTGATGACATTCAACAAGAAAGAAGATTTTGCTGCATTTCAGAGCCATCCAAGTCATGTTGAGTTCTCTGAAAAGTTTTCAACAGCTATTGAAAAGATTGTGCTGCTTGATTTCCCTTCAACCCTTGTCAAGCCAGTCAAGGAAGCAGCATAA

Protein sequence

>MS.gene90649.t1

MVVMEEFKHFVIVKFKEGVVVEELTKGMEKLVSEIDLVKSFEWGQDIESLDVLRQGFTHAFLMTFNKKEDFAAFQSHPSHVEFSEKFSTAIEKIVLLDFPSTLVKPVKEAA