AlfalfaGEDB | MS.gene073085

M. sativa cultivar XinJiangDaYe / MS.gene073085

KEGG: -
GO: -
PFAM: PF07876.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073085.t1	XP_013464923.1	99.1	111	1	0	1	111	1	111	5.40E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073085.t1	Q9FK81	65.7	99	34	0	7	105	6	104	1.7e-30	133.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073085.t1	B7FMZ0	99.1	111	1	0	1	111	1	111	3.9e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049341	MS.gene073085	0.811116	8.22E-51	-1.69E-46
MS.gene049501	MS.gene073085	0.800861	1.18E-48	-1.69E-46
MS.gene049850	MS.gene073085	0.815427	9.30E-52	-1.69E-46
MS.gene049979	MS.gene073085	0.801233	9.92E-49	-1.69E-46
MS.gene050020	MS.gene073085	0.834557	2.80E-56	-1.69E-46
MS.gene05016	MS.gene073085	0.803962	2.71E-49	-1.69E-46
MS.gene05045	MS.gene073085	0.809471	1.86E-50	-1.69E-46
MS.gene050586	MS.gene073085	0.801107	1.05E-48	-1.69E-46
MS.gene050807	MS.gene073085	0.859722	3.40E-63	-1.69E-46
MS.gene050808	MS.gene073085	0.810034	1.41E-50	-1.69E-46
MS.gene050815	MS.gene073085	0.817715	2.86E-52	-1.69E-46
MS.gene050844	MS.gene073085	0.809264	2.06E-50	-1.69E-46
MS.gene051247	MS.gene073085	0.815321	9.81E-52	-1.69E-46
MS.gene051265	MS.gene073085	0.802937	4.42E-49	-1.69E-46
MS.gene051328	MS.gene073085	0.802104	6.57E-49	-1.69E-46
MS.gene05157	MS.gene073085	0.823847	1.11E-53	-1.69E-46
MS.gene051719	MS.gene073085	0.846776	1.76E-59	-1.69E-46
MS.gene051720	MS.gene073085	0.827853	1.24E-54	-1.69E-46
MS.gene051744	MS.gene073085	0.803288	3.74E-49	-1.69E-46
MS.gene051789	MS.gene073085	0.822089	2.85E-53	-1.69E-46
MS.gene052020	MS.gene073085	0.808109	3.64E-50	-1.69E-46
MS.gene052133	MS.gene073085	0.813257	2.81E-51	-1.69E-46
MS.gene052516	MS.gene073085	0.80232	5.93E-49	-1.69E-46
MS.gene052611	MS.gene073085	0.852098	5.77E-61	-1.69E-46
MS.gene052612	MS.gene073085	0.807369	5.23E-50	-1.69E-46
MS.gene052665	MS.gene073085	0.819126	1.37E-52	-1.69E-46
MS.gene052863	MS.gene073085	0.811283	7.57E-51	-1.69E-46
MS.gene052879	MS.gene073085	0.806532	7.86E-50	-1.69E-46
MS.gene052896	MS.gene073085	0.821857	3.23E-53	-1.69E-46
MS.gene053074	MS.gene073085	0.819402	1.18E-52	-1.69E-46
MS.gene053144	MS.gene073085	0.838331	3.06E-57	-1.69E-46
MS.gene053174	MS.gene073085	0.811213	7.83E-51	-1.69E-46
MS.gene053563	MS.gene073085	0.812261	4.63E-51	-1.69E-46
MS.gene053581	MS.gene073085	0.847143	1.39E-59	-1.69E-46
MS.gene054472	MS.gene073085	0.812816	3.50E-51	-1.69E-46
MS.gene054550	MS.gene073085	0.804944	1.69E-49	-1.69E-46
MS.gene05456	MS.gene073085	0.805293	1.43E-49	-1.69E-46
MS.gene054584	MS.gene073085	0.827367	1.63E-54	-1.69E-46
MS.gene055465	MS.gene073085	0.81013	1.34E-50	-1.69E-46
MS.gene055466	MS.gene073085	0.83806	3.60E-57	-1.69E-46
MS.gene055468	MS.gene073085	0.825324	4.99E-54	-1.69E-46
MS.gene055512	MS.gene073085	0.823346	1.46E-53	-1.69E-46
MS.gene055580	MS.gene073085	-0.814216	1.72E-51	-1.69E-46
MS.gene055818	MS.gene073085	0.834054	3.74E-56	-1.69E-46
MS.gene055890	MS.gene073085	0.822328	2.51E-53	-1.69E-46
MS.gene055891	MS.gene073085	0.813061	3.10E-51	-1.69E-46
MS.gene056005	MS.gene073085	0.831554	1.56E-55	-1.69E-46
MS.gene056069	MS.gene073085	-0.803238	3.84E-49	-1.69E-46
MS.gene056366	MS.gene073085	0.82018	7.86E-53	-1.69E-46
MS.gene056542	MS.gene073085	0.840347	9.19E-58	-1.69E-46
MS.gene056543	MS.gene073085	0.845054	5.16E-59	-1.69E-46
MS.gene056545	MS.gene073085	0.851215	1.03E-60	-1.69E-46
MS.gene056553	MS.gene073085	0.848585	5.58E-60	-1.69E-46
MS.gene056760	MS.gene073085	0.811594	6.47E-51	-1.69E-46
MS.gene058168	MS.gene073085	0.826602	2.48E-54	-1.69E-46
MS.gene058625	MS.gene073085	0.805306	1.42E-49	-1.69E-46
MS.gene058906	MS.gene073085	0.830654	2.60E-55	-1.69E-46
MS.gene059006	MS.gene073085	0.806269	8.93E-50	-1.69E-46
MS.gene059937	MS.gene073085	0.822107	2.83E-53	-1.69E-46
MS.gene060587	MS.gene073085	0.807785	4.27E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene073085	MS.gene24782	PPI
MS.gene25266	MS.gene073085	PPI
MS.gene073085	MS.gene012190	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073085.t1	MTR_2g084985	99.099	111	1	0	1	111	1	111	6.00e-75	217
MS.gene073085.t1	MTR_4g124920	41.584	101	55	2	8	105	4	103	1.40e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073085.t1	AT5G22580	65.657	99	34	0	7	105	6	104	5.30e-42	134
MS.gene073085.t1	AT3G17210	42.000	100	54	2	8	104	9	107	3.39e-22	84.0
MS.gene073085.t1	AT3G17210	42.000	100	54	2	8	104	9	107	3.39e-22	84.0

Find 23 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATTATGCTGCTTCCTTAAC+TGG	0.195704	2.2:-12608779	None:intergenic
TACAAAAGGGATGGAGAAAT+TGG	0.322091	2.2:+12607906	MS.gene073085:CDS
ATCTTGTCAAATCCTTTGAA+TGG	0.422994	2.2:+12607941	MS.gene073085:CDS
AACTGGCTTGACAAGGGTTG+AGG	0.423667	2.2:-12608762	None:intergenic
AACCCTTGTCAAGCCAGTTA+AGG	0.431460	2.2:+12608766	MS.gene073085:CDS
CTTCCTTAACTGGCTTGACA+AGG	0.454173	2.2:-12608769	None:intergenic
CTTGTTGAATGTCATCAAGA+AGG	0.457412	2.2:-12608642	None:intergenic
GTGATTGTTAAGTTCAAAGA+AGG	0.458164	2.2:+12607865	MS.gene073085:CDS
GTAGTGGAAGAGCTTACAAA+AGG	0.480357	2.2:+12607892	MS.gene073085:CDS
GTTCAAAGAAGGTGTAGTAG+TGG	0.481023	2.2:+12607876	MS.gene073085:CDS
TCAGAGAACTCAACATGACT+TGG	0.494285	2.2:-12608695	None:intergenic
ACTGGCTTGACAAGGGTTGA+GGG	0.505995	2.2:-12608761	None:intergenic
AACCTTGTCTCAACACATCT+AGG	0.520013	2.2:-12608610	None:intergenic
TTTCTAATCATACAGGGGAC+AGG	0.545053	2.2:+12608577	MS.gene073085:intron
AGAACTCAACATGACTTGGA+TGG	0.554383	2.2:-12608691	None:intergenic
TTCACAAAGAATGGTAGTCA+TGG	0.559784	2.2:+12607825	None:intergenic
TTCCTTAACTGGCTTGACAA+GGG	0.564730	2.2:-12608768	None:intergenic
AGCCTAGATGTGTTGAGACA+AGG	0.579475	2.2:+12608608	MS.gene073085:CDS
CTGGCTTGACAAGGGTTGAG+GGG	0.581401	2.2:-12608760	None:intergenic
TAGTGGAAGAGCTTACAAAA+GGG	0.588758	2.2:+12607893	MS.gene073085:CDS
GGAAGAGCTTACAAAAGGGA+TGG	0.589362	2.2:+12607897	MS.gene073085:CDS
GTATAAGACATACCATTCAA+AGG	0.611465	2.2:-12607953	None:intergenic
ACAAAGAATGGTAGTCATGG+AGG	0.636423	2.2:+12607828	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATACAATTATTTATATTGA+CGG	-	chr2.2:12608397-12608416	None:intergenic	10.0%
!!	AATTACTAGTTATAAGTAAT+CGG	+	chr2.2:12608459-12608478	MS.gene073085:intron	15.0%
!!!	ACATAAAAAAATTTGATTTC+AGG	+	chr2.2:12608306-12608325	MS.gene073085:intron	15.0%
!!	ACTGAGATTTATAGTTAAAT+AGG	+	chr2.2:12608252-12608271	MS.gene073085:intron	20.0%
!!	ATATGATAGTACTATAAAGA+GGG	-	chr2.2:12608549-12608568	None:intergenic	20.0%
!!	TATAAATCTCAGTAAAATCA+TGG	-	chr2.2:12608245-12608264	None:intergenic	20.0%
!	AAAAACACTATCTGTATCAA+AGG	-	chr2.2:12608068-12608087	None:intergenic	25.0%
!	AAACAGAAATACAAGAACAA+GGG	+	chr2.2:12608121-12608140	MS.gene073085:intron	25.0%
!	ATAAAGCTTTCTAATCATAC+AGG	+	chr2.2:12608570-12608589	MS.gene073085:intron	25.0%
!	ATCAAAGGTTAATAAACTGA+AGG	-	chr2.2:12608053-12608072	None:intergenic	25.0%
!	ATGTGACGATTATGATTATT+TGG	+	chr2.2:12608498-12608517	MS.gene073085:intron	25.0%
!	GATATGATAGTACTATAAAG+AGG	-	chr2.2:12608550-12608569	None:intergenic	25.0%
!	TAAAGCTTTCTAATCATACA+GGG	+	chr2.2:12608571-12608590	MS.gene073085:intron	25.0%
!	TCTCATGTACTTAAATATCA+AGG	+	chr2.2:12608176-12608195	MS.gene073085:intron	25.0%
!	TCTGTTATCTAATATATCAG+TGG	-	chr2.2:12608102-12608121	None:intergenic	25.0%
!!!	AATATCAAGGTTTTAGATTG+AGG	+	chr2.2:12608189-12608208	MS.gene073085:intron	25.0%
!!!	ATTTTTTTATGTTTTCGCGT+TGG	-	chr2.2:12608298-12608317	None:intergenic	25.0%
	AAAGCTTTCTAATCATACAG+GGG	+	chr2.2:12608572-12608591	MS.gene073085:intron	30.0%
	ATCTTGTCAAATCCTTTGAA+TGG	+	chr2.2:12607941-12607960	MS.gene073085:CDS	30.0%
	GAAACAGAAATACAAGAACA+AGG	+	chr2.2:12608120-12608139	MS.gene073085:intron	30.0%
	GTATAAGACATACCATTCAA+AGG	-	chr2.2:12607956-12607975	None:intergenic	30.0%
	GTGATTGTTAAGTTCAAAGA+AGG	+	chr2.2:12607865-12607884	MS.gene073085:CDS	30.0%
!	TACCTTTTATATTGTGCAAG+AGG	+	chr2.2:12607979-12607998	MS.gene073085:intron	30.0%
	AGCCTCTTGCACAATATAAA+AGG	-	chr2.2:12607984-12608003	None:intergenic	35.0%
	CTTGTTGAATGTCATCAAGA+AGG	-	chr2.2:12608645-12608664	None:intergenic	35.0%
	TACAAAAGGGATGGAGAAAT+TGG	+	chr2.2:12607906-12607925	MS.gene073085:CDS	35.0%
	TAGTGGAAGAGCTTACAAAA+GGG	+	chr2.2:12607893-12607912	MS.gene073085:CDS	35.0%
	TTGATTTCAGGCTTACATCA+CGG	+	chr2.2:12608318-12608337	MS.gene073085:intron	35.0%
	AACCTTGTCTCAACACATCT+AGG	-	chr2.2:12608613-12608632	None:intergenic	40.0%
	AGTTAAATAGGCCTCGATTG+TGG	+	chr2.2:12608264-12608283	MS.gene073085:intron	40.0%
	TCAGAGAACTCAACATGACT+TGG	-	chr2.2:12608698-12608717	None:intergenic	40.0%
	TTTCTAATCATACAGGGGAC+AGG	+	chr2.2:12608577-12608596	MS.gene073085:intron	40.0%
!	AGAACTCAACATGACTTGGA+TGG	-	chr2.2:12608694-12608713	None:intergenic	40.0%
!	GTAGTGGAAGAGCTTACAAA+AGG	+	chr2.2:12607892-12607911	MS.gene073085:CDS	40.0%
!	GTTCAAAGAAGGTGTAGTAG+TGG	+	chr2.2:12607876-12607895	MS.gene073085:CDS	40.0%
!!	TTCCTTAACTGGCTTGACAA+GGG	-	chr2.2:12608771-12608790	None:intergenic	40.0%
!!!	ATGTTTTCGCGTTGGTAATG+TGG	-	chr2.2:12608290-12608309	None:intergenic	40.0%
	AACCCTTGTCAAGCCAGTTA+AGG	+	chr2.2:12608766-12608785	MS.gene073085:CDS	45.0%
	AGCCTAGATGTGTTGAGACA+AGG	+	chr2.2:12608608-12608627	MS.gene073085:CDS	45.0%
	GGAAGAGCTTACAAAAGGGA+TGG	+	chr2.2:12607897-12607916	MS.gene073085:CDS	45.0%
!!	CTTCCTTAACTGGCTTGACA+AGG	-	chr2.2:12608772-12608791	None:intergenic	45.0%
!	TGGTAATGTGGCCACAATCG+AGG	-	chr2.2:12608278-12608297	None:intergenic	50.0%
!!	AACTGGCTTGACAAGGGTTG+AGG	-	chr2.2:12608765-12608784	None:intergenic	50.0%
!!	ACTGGCTTGACAAGGGTTGA+GGG	-	chr2.2:12608764-12608783	None:intergenic	50.0%
	CGGTCGTAGACTCGTAGTTG+TGG	+	chr2.2:12608338-12608357	MS.gene073085:intron	55.0%
!	CTGGCTTGACAAGGGTTGAG+GGG	-	chr2.2:12608763-12608782	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	12607835	12608799	12607835	ID=MS.gene073085
chr2.2	mRNA	12607835	12608799	12607835	ID=MS.gene073085.t1;Parent=MS.gene073085
chr2.2	exon	12607835	12607962	12607835	ID=MS.gene073085.t1.exon1;Parent=MS.gene073085.t1
chr2.2	CDS	12607835	12607962	12607835	ID=cds.MS.gene073085.t1;Parent=MS.gene073085.t1
chr2.2	exon	12608592	12608799	12608592	ID=MS.gene073085.t1.exon2;Parent=MS.gene073085.t1
chr2.2	CDS	12608592	12608799	12608592	ID=cds.MS.gene073085.t1;Parent=MS.gene073085.t1

Gene Sequence

>MS.gene073085

ATGGTAGTCATGGAGGAATTCAAGCACTTTGTGATTGTTAAGTTCAAAGAAGGTGTAGTAGTGGAAGAGCTTACAAAAGGGATGGAGAAATTGGTCTCAGAGATTGATCTTGTCAAATCCTTTGAATGGTATGTCTTATACATTTACCTTTTATATTGTGCAAGAGGCTTTATACTTCAGTAAACATAAGAGCAAGTAGTTAAACATTCTCAAGTCCTTCAGTTTATTAACCTTTGATACAGATAGTGTTTTTATTGAGTTACACCACTGATATATTAGATAACAGAAACAGAAATACAAGAACAAGGGTTCAAAGTAGAACAAAGACAATTAATCATAGTTCTCATGTACTTAAATATCAAGGTTTTAGATTGAGGTTGCTGTCGTGTTCACAGTTTTATTGCAATCCATGATTTTACTGAGATTTATAGTTAAATAGGCCTCGATTGTGGCCACATTACCAACGCGAAAACATAAAAAAATTTGATTTCAGGCTTACATCACGGTCGTAGACTCGTAGTTGTGGATATTTTTTTTTCTAGAGTTGAAGTTTGATATACCGTCAATATAAATAATTGTATACTGTCATCAAGTCACAACTAATCATATATAATATGACTTTAAAATTACTAGTTATAAGTAATCGGTTTTTAACGATGTGAGATGTGACGATTATGATTATTTGGCATTGTAAAAAATCTTTGCACAAAACCCTCTTTATAGTACTATCATATCATAAAGCTTTCTAATCATACAGGGGACAGGATATTGAAAGCCTAGATGTGTTGAGACAAGGTTTCACTCATGCCTTCTTGATGACATTCAACAAGAAAGAAGATTTTGCTGCATTTCAGAGCCATCCAAGTCATGTTGAGTTCTCTGAAAAGTTTTCAACAGCTATTGAAAAGATTGTGCTGCTTGATTTCCCCTCAACCCTTGTCAAGCCAGTTAAGGAAGCAGCATAA

Protein sequence

>MS.gene073085.t1

MVVMEEFKHFVIVKFKEGVVVEELTKGMEKLVSEIDLVKSFEWGQDIESLDVLRQGFTHAFLMTFNKKEDFAAFQSHPSHVEFSEKFSTAIEKIVLLDFPSTLVKPVKEAA