Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35373.t1 | XP_013464923.1 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 5.40E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35373.t1 | Q9FK81 | 65.7 | 99 | 34 | 0 | 7 | 105 | 6 | 104 | 1.7e-30 | 133.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35373.t1 | B7FMZ0 | 99.1 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 3.9e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene35373 | 0.80445 | 2.15E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene35373 | 0.842322 | 2.78E-58 | -1.69E-46 |
| MS.gene050780 | MS.gene35373 | 0.814078 | 1.85E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene35373 | 0.852679 | 3.94E-61 | -1.69E-46 |
| MS.gene050808 | MS.gene35373 | 0.830426 | 2.96E-55 | -1.69E-46 |
| MS.gene051247 | MS.gene35373 | 0.824216 | 9.10E-54 | -1.69E-46 |
| MS.gene051265 | MS.gene35373 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene35373 | 0.801595 | 8.36E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene35373 | 0.82731 | 1.68E-54 | -1.69E-46 |
| MS.gene052020 | MS.gene35373 | 0.810732 | 9.96E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene35373 | 0.817083 | 3.96E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene35373 | 0.800489 | 1.41E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene35373 | 0.804933 | 1.70E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene35373 | 0.807756 | 4.33E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene35373 | 0.817309 | 3.53E-52 | -1.69E-46 |
| MS.gene053383 | MS.gene35373 | 0.802186 | 6.32E-49 | -1.69E-46 |
| MS.gene053563 | MS.gene35373 | 0.822787 | 1.96E-53 | -1.69E-46 |
| MS.gene053630 | MS.gene35373 | 0.815993 | 6.95E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene35373 | 0.8124 | 4.32E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene35373 | 0.815705 | 8.06E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene35373 | 0.809982 | 1.45E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene35373 | 0.821026 | 5.02E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene35373 | 0.80444 | 2.16E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene35373 | 0.820641 | 6.16E-53 | -1.69E-46 |
| MS.gene056005 | MS.gene35373 | 0.810631 | 1.05E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene35373 | -0.816804 | 4.58E-52 | -1.69E-46 |
| MS.gene057047 | MS.gene35373 | 0.819965 | 8.80E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene35373 | 0.833502 | 5.14E-56 | -1.69E-46 |
| MS.gene058168 | MS.gene35373 | 0.812212 | 4.75E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene35373 | 0.811649 | 6.30E-51 | -1.69E-46 |
| MS.gene059725 | MS.gene35373 | 0.802138 | 6.47E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene35373 | 0.804811 | 1.81E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene35373 | 0.802171 | 6.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35373 | MS.gene25266 | PPI |
| MS.gene35373 | MS.gene012190 | PPI |
| MS.gene35373 | MS.gene24782 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35373.t1 | MTR_2g084985 | 99.099 | 111 | 1 | 0 | 1 | 111 | 1 | 111 | 6.00e-75 | 217 |
| MS.gene35373.t1 | MTR_4g124920 | 41.584 | 101 | 55 | 2 | 8 | 105 | 4 | 103 | 1.40e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35373.t1 | AT5G22580 | 65.657 | 99 | 34 | 0 | 7 | 105 | 6 | 104 | 5.30e-42 | 134 |
| MS.gene35373.t1 | AT3G17210 | 42.000 | 100 | 54 | 2 | 8 | 104 | 9 | 107 | 3.39e-22 | 84.0 |
| MS.gene35373.t1 | AT3G17210 | 42.000 | 100 | 54 | 2 | 8 | 104 | 9 | 107 | 3.39e-22 | 84.0 |
Find 23 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATGCTGCTTCCTTGAC+TGG | 0.289662 | 2.1:-16443505 | None:intergenic |
| TACAAAAGGGATGGAGAAAT+TGG | 0.322091 | 2.1:+16442666 | MS.gene35373:CDS |
| ACTGGCTTGACAAGGGTTGA+TGG | 0.410675 | 2.1:-16443487 | None:intergenic |
| CTTCCTTGACTGGCTTGACA+AGG | 0.415501 | 2.1:-16443495 | None:intergenic |
| ATCTTGTCAAATCCTTTGAA+TGG | 0.422994 | 2.1:+16442701 | MS.gene35373:CDS |
| CTTGTTGAATGTCATCAAGA+AGG | 0.457412 | 2.1:-16443368 | None:intergenic |
| GTGATTGTTAAGTTCAAAGA+AGG | 0.458164 | 2.1:+16442625 | MS.gene35373:CDS |
| GTAGTGGAAGAGCTTACAAA+AGG | 0.480357 | 2.1:+16442652 | MS.gene35373:CDS |
| GTTCAAAGAAGGTGTAGTAG+TGG | 0.481023 | 2.1:+16442636 | MS.gene35373:CDS |
| GGGACAGGATATTGAAAGCC+TGG | 0.493701 | 2.1:+16443318 | MS.gene35373:CDS |
| TCAGAGAACTCAACATGACT+TGG | 0.494285 | 2.1:-16443421 | None:intergenic |
| AACCTTGTCTCAACACATCC+AGG | 0.501843 | 2.1:-16443336 | None:intergenic |
| CTGGCTTGACAAGGGTTGAT+GGG | 0.538605 | 2.1:-16443486 | None:intergenic |
| TTCCTTGACTGGCTTGACAA+GGG | 0.547981 | 2.1:-16443494 | None:intergenic |
| AGAACTCAACATGACTTGGA+TGG | 0.554383 | 2.1:-16443417 | None:intergenic |
| GTATGTCTTATACAGGGGAC+AGG | 0.555403 | 2.1:+16443303 | MS.gene35373:intron |
| TTCACAAAGAATGGTAGTCA+TGG | 0.559784 | 2.1:+16442585 | None:intergenic |
| GTATAAAACATACCATTCAA+AGG | 0.584418 | 2.1:-16442713 | None:intergenic |
| AACCCTTGTCAAGCCAGTCA+AGG | 0.586460 | 2.1:+16443492 | MS.gene35373:CDS |
| TAGTGGAAGAGCTTACAAAA+GGG | 0.588758 | 2.1:+16442653 | MS.gene35373:CDS |
| GGAAGAGCTTACAAAAGGGA+TGG | 0.589362 | 2.1:+16442657 | MS.gene35373:CDS |
| AGCCTGGATGTGTTGAGACA+AGG | 0.635747 | 2.1:+16443334 | MS.gene35373:CDS |
| ACAAAGAATGGTAGTCATGG+AGG | 0.636423 | 2.1:+16442588 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTATCAGTATCTATA+AGG | - | chr2.1:16442831-16442850 | None:intergenic | 20.0% |
| !! | AACAAAGACAGTAATTTAAA+GGG | + | chr2.1:16442914-16442933 | MS.gene35373:intron | 20.0% |
| !! | ATGTGATGATTATGATTATT+TGG | + | chr2.1:16443216-16443235 | MS.gene35373:intron | 20.0% |
| !!! | ACATCAAAAGATTTGATTTT+GGG | + | chr2.1:16443034-16443053 | MS.gene35373:intron | 20.0% |
| ! | AACAGAAACAGAAATAACAA+GGG | + | chr2.1:16442881-16442900 | MS.gene35373:intron | 25.0% |
| ! | AATCAAGTATGTCTTATACA+GGG | + | chr2.1:16443297-16443316 | MS.gene35373:intron | 25.0% |
| ! | ACTGAGATTTATAGCTAAAT+AGG | + | chr2.1:16442980-16442999 | MS.gene35373:intron | 25.0% |
| ! | ATGATATGATAGTACTAAGT+AGG | - | chr2.1:16443268-16443287 | None:intergenic | 25.0% |
| ! | CTATAAGGTTAATAAACTGA+AGG | - | chr2.1:16442816-16442835 | None:intergenic | 25.0% |
| ! | GAACAAAGACAGTAATTTAA+AGG | + | chr2.1:16442913-16442932 | MS.gene35373:intron | 25.0% |
| ! | GTATAAAACATACCATTCAA+AGG | - | chr2.1:16442716-16442735 | None:intergenic | 25.0% |
| ! | TAACAGAAACAGAAATAACA+AGG | + | chr2.1:16442880-16442899 | MS.gene35373:intron | 25.0% |
| ! | TAATCAAGTATGTCTTATAC+AGG | + | chr2.1:16443296-16443315 | MS.gene35373:intron | 25.0% |
| ! | TCTGTTATCAAATATATCAG+TGG | - | chr2.1:16442867-16442886 | None:intergenic | 25.0% |
| ! | TGATATGATAGTACTAAGTA+GGG | - | chr2.1:16443267-16443286 | None:intergenic | 25.0% |
| !! | TACCTTTTATATTATGCAAG+AGG | + | chr2.1:16442739-16442758 | MS.gene35373:intron | 25.0% |
| !!! | GACATCAAAAGATTTGATTT+TGG | + | chr2.1:16443033-16443052 | MS.gene35373:intron | 25.0% |
| ATCAAGTATGTCTTATACAG+GGG | + | chr2.1:16443298-16443317 | MS.gene35373:intron | 30.0% | |
| ATCTTGTCAAATCCTTTGAA+TGG | + | chr2.1:16442701-16442720 | MS.gene35373:CDS | 30.0% | |
| GTGATTGTTAAGTTCAAAGA+AGG | + | chr2.1:16442625-16442644 | MS.gene35373:CDS | 30.0% | |
| TGCCTCTTGCATAATATAAA+AGG | - | chr2.1:16442744-16442763 | None:intergenic | 30.0% | |
| CTTGTTGAATGTCATCAAGA+AGG | - | chr2.1:16443371-16443390 | None:intergenic | 35.0% | |
| TACAAAAGGGATGGAGAAAT+TGG | + | chr2.1:16442666-16442685 | MS.gene35373:CDS | 35.0% | |
| TAGTGGAAGAGCTTACAAAA+GGG | + | chr2.1:16442653-16442672 | MS.gene35373:CDS | 35.0% | |
| ! | GTATACGATTAGTTGTGACT+TGG | - | chr2.1:16443152-16443171 | None:intergenic | 35.0% |
| ! | TTGATTTTGGGCTTACATCA+CGG | + | chr2.1:16443046-16443065 | MS.gene35373:intron | 35.0% |
| TCAGAGAACTCAACATGACT+TGG | - | chr2.1:16443424-16443443 | None:intergenic | 40.0% | |
| ! | AGAACTCAACATGACTTGGA+TGG | - | chr2.1:16443420-16443439 | None:intergenic | 40.0% |
| ! | ATCTTTTGATGTCTTCGCGT+TGG | - | chr2.1:16443026-16443045 | None:intergenic | 40.0% |
| ! | GTAGTGGAAGAGCTTACAAA+AGG | + | chr2.1:16442652-16442671 | MS.gene35373:CDS | 40.0% |
| ! | GTTCAAAGAAGGTGTAGTAG+TGG | + | chr2.1:16442636-16442655 | MS.gene35373:CDS | 40.0% |
| !! | GATTAGTTGTGACTTGGTGT+CGG | - | chr2.1:16443146-16443165 | None:intergenic | 40.0% |
| AACCTTGTCTCAACACATCC+AGG | - | chr2.1:16443339-16443358 | None:intergenic | 45.0% | |
| AGCTAAATAGGCCTCGATTG+CGG | + | chr2.1:16442992-16443011 | MS.gene35373:intron | 45.0% | |
| CGGTTGTAGACTCGTAGTTA+TGG | + | chr2.1:16443066-16443085 | MS.gene35373:intron | 45.0% | |
| GGAAGAGCTTACAAAAGGGA+TGG | + | chr2.1:16442657-16442676 | MS.gene35373:CDS | 45.0% | |
| GTATGTCTTATACAGGGGAC+AGG | + | chr2.1:16443303-16443322 | MS.gene35373:intron | 45.0% | |
| !! | ATGTCTTCGCGTTGGTAATG+TGG | - | chr2.1:16443018-16443037 | None:intergenic | 45.0% |
| !! | TTCCTTGACTGGCTTGACAA+GGG | - | chr2.1:16443497-16443516 | None:intergenic | 45.0% |
| AACCCTTGTCAAGCCAGTCA+AGG | + | chr2.1:16443492-16443511 | MS.gene35373:CDS | 50.0% | |
| AAGGGAAATTCCCCAACCAG+AGG | + | chr2.1:16442932-16442951 | MS.gene35373:intron | 50.0% | |
| AATTCCCCAACCAGAGGTTG+CGG | + | chr2.1:16442938-16442957 | MS.gene35373:intron | 50.0% | |
| AGAGGTTGCGGTCATGTTCA+CGG | + | chr2.1:16442950-16442969 | MS.gene35373:intron | 50.0% | |
| AGCCTGGATGTGTTGAGACA+AGG | + | chr2.1:16443334-16443353 | MS.gene35373:CDS | 50.0% | |
| GGGACAGGATATTGAAAGCC+TGG | + | chr2.1:16443318-16443337 | MS.gene35373:CDS | 50.0% | |
| ! | CTGGCTTGACAAGGGTTGAT+GGG | - | chr2.1:16443489-16443508 | None:intergenic | 50.0% |
| !! | ACTGGCTTGACAAGGGTTGA+TGG | - | chr2.1:16443490-16443509 | None:intergenic | 50.0% |
| !! | CTTCCTTGACTGGCTTGACA+AGG | - | chr2.1:16443498-16443517 | None:intergenic | 50.0% |
| ACATGACCGCAACCTCTGGT+TGG | - | chr2.1:16442947-16442966 | None:intergenic | 55.0% | |
| ATGACCGCAACCTCTGGTTG+GGG | - | chr2.1:16442945-16442964 | None:intergenic | 55.0% | |
| CATGACCGCAACCTCTGGTT+GGG | - | chr2.1:16442946-16442965 | None:intergenic | 55.0% | |
| GTGAACATGACCGCAACCTC+TGG | - | chr2.1:16442951-16442970 | None:intergenic | 55.0% | |
| ! | TGGTAATGTGGCCGCAATCG+AGG | - | chr2.1:16443006-16443025 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 16442595 | 16443525 | 16442595 | ID=MS.gene35373 |
| chr2.1 | mRNA | 16442595 | 16443525 | 16442595 | ID=MS.gene35373.t1;Parent=MS.gene35373 |
| chr2.1 | exon | 16442595 | 16442722 | 16442595 | ID=MS.gene35373.t1.exon1;Parent=MS.gene35373.t1 |
| chr2.1 | CDS | 16442595 | 16442722 | 16442595 | ID=cds.MS.gene35373.t1;Parent=MS.gene35373.t1 |
| chr2.1 | exon | 16443318 | 16443525 | 16443318 | ID=MS.gene35373.t1.exon2;Parent=MS.gene35373.t1 |
| chr2.1 | CDS | 16443318 | 16443525 | 16443318 | ID=cds.MS.gene35373.t1;Parent=MS.gene35373.t1 |
| Gene Sequence |
| Protein sequence |
