Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012473.t1 | XP_013458563.1 | 99.8 | 404 | 1 | 0 | 1 | 404 | 1 | 404 | 1.10E-230 | 808.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012473.t1 | Q9M0B6 | 85.5 | 399 | 54 | 1 | 6 | 404 | 3 | 397 | 1.8e-202 | 706.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012473.t1 | A0A072US06 | 99.8 | 404 | 1 | 0 | 1 | 404 | 1 | 404 | 7.7e-231 | 808.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012473 | MS.gene99045 | PPI |
| MS.gene066580 | MS.gene012473 | PPI |
| MS.gene48984 | MS.gene012473 | PPI |
| MS.gene012473 | MS.gene50266 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012473.t1 | MTR_4g129820 | 99.752 | 404 | 1 | 0 | 1 | 404 | 1 | 404 | 0.0 | 834 |
| MS.gene012473.t1 | MTR_4g024600 | 67.241 | 406 | 123 | 4 | 6 | 404 | 8 | 410 | 0.0 | 548 |
| MS.gene012473.t1 | MTR_0354s0040 | 67.254 | 397 | 116 | 4 | 13 | 404 | 4 | 391 | 0.0 | 543 |
| MS.gene012473.t1 | MTR_4g102270 | 61.671 | 407 | 144 | 3 | 4 | 404 | 2 | 402 | 0.0 | 513 |
| MS.gene012473.t1 | MTR_3g084090 | 64.444 | 405 | 130 | 5 | 13 | 404 | 5 | 408 | 0.0 | 513 |
| MS.gene012473.t1 | MTR_5g011940 | 70.710 | 338 | 97 | 1 | 73 | 408 | 66 | 403 | 5.62e-175 | 495 |
| MS.gene012473.t1 | MTR_2g032100 | 26.727 | 333 | 211 | 10 | 89 | 403 | 65 | 382 | 7.57e-22 | 97.1 |
| MS.gene012473.t1 | MTR_2g073240 | 30.962 | 239 | 143 | 5 | 96 | 324 | 20 | 246 | 2.83e-21 | 96.7 |
| MS.gene012473.t1 | MTR_0034s0060 | 31.330 | 233 | 150 | 5 | 96 | 324 | 138 | 364 | 3.41e-21 | 96.7 |
| MS.gene012473.t1 | MTR_2g032100 | 30.612 | 196 | 132 | 3 | 89 | 282 | 65 | 258 | 2.57e-20 | 90.9 |
| MS.gene012473.t1 | MTR_8g099535 | 28.358 | 335 | 190 | 14 | 96 | 403 | 5 | 316 | 4.56e-20 | 90.9 |
| MS.gene012473.t1 | MTR_5g009170 | 27.523 | 327 | 203 | 12 | 96 | 403 | 48 | 359 | 6.79e-19 | 88.2 |
| MS.gene012473.t1 | MTR_1g009600 | 26.351 | 296 | 181 | 7 | 96 | 380 | 6 | 275 | 6.82e-19 | 89.4 |
| MS.gene012473.t1 | MTR_8g089180 | 25.749 | 334 | 211 | 11 | 90 | 403 | 66 | 382 | 1.80e-17 | 84.0 |
| MS.gene012473.t1 | MTR_8g089180 | 25.749 | 334 | 211 | 11 | 90 | 403 | 66 | 382 | 1.80e-17 | 84.0 |
| MS.gene012473.t1 | MTR_4g063600 | 26.846 | 298 | 181 | 11 | 92 | 382 | 29 | 296 | 8.17e-17 | 81.6 |
| MS.gene012473.t1 | MTR_5g009160 | 26.586 | 331 | 201 | 13 | 96 | 403 | 5 | 316 | 9.66e-17 | 81.3 |
| MS.gene012473.t1 | MTR_1g088480 | 26.923 | 338 | 192 | 15 | 84 | 404 | 98 | 397 | 2.22e-16 | 80.9 |
| MS.gene012473.t1 | MTR_8g096880 | 34.356 | 163 | 101 | 4 | 97 | 256 | 8 | 167 | 8.89e-16 | 77.8 |
| MS.gene012473.t1 | MTR_8g096880 | 26.586 | 331 | 203 | 12 | 97 | 404 | 8 | 321 | 1.10e-15 | 77.8 |
| MS.gene012473.t1 | MTR_8g096880 | 26.586 | 331 | 203 | 12 | 97 | 404 | 8 | 321 | 1.26e-15 | 78.2 |
| MS.gene012473.t1 | MTR_4g063600 | 27.181 | 298 | 177 | 12 | 92 | 382 | 29 | 293 | 2.65e-15 | 77.0 |
| MS.gene012473.t1 | MTR_4g120030 | 24.474 | 380 | 224 | 15 | 27 | 384 | 37 | 375 | 7.69e-15 | 76.3 |
| MS.gene012473.t1 | MTR_4g134490 | 25.000 | 352 | 216 | 12 | 79 | 404 | 11 | 340 | 9.73e-15 | 75.5 |
| MS.gene012473.t1 | MTR_4g134490 | 25.000 | 352 | 216 | 12 | 79 | 404 | 11 | 340 | 1.14e-14 | 75.5 |
| MS.gene012473.t1 | MTR_7g115080 | 25.926 | 243 | 154 | 8 | 95 | 326 | 29 | 256 | 1.96e-11 | 65.5 |
| MS.gene012473.t1 | MTR_7g115080 | 25.926 | 243 | 154 | 8 | 95 | 326 | 63 | 290 | 2.56e-11 | 65.5 |
| MS.gene012473.t1 | MTR_7g115080 | 25.926 | 243 | 154 | 8 | 95 | 326 | 42 | 269 | 2.80e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012473.t1 | AT4G30440 | 85.464 | 399 | 54 | 1 | 6 | 404 | 3 | 397 | 0.0 | 701 |
| MS.gene012473.t1 | AT1G02000 | 68.812 | 404 | 118 | 5 | 5 | 404 | 2 | 401 | 0.0 | 557 |
| MS.gene012473.t1 | AT4G00110 | 68.127 | 411 | 113 | 5 | 1 | 404 | 1 | 400 | 0.0 | 554 |
| MS.gene012473.t1 | AT2G45310 | 65.777 | 412 | 122 | 5 | 5 | 404 | 2 | 406 | 0.0 | 530 |
| MS.gene012473.t1 | AT3G23820 | 72.424 | 330 | 91 | 0 | 75 | 404 | 92 | 421 | 1.35e-179 | 508 |
| MS.gene012473.t1 | AT4G12250 | 65.281 | 409 | 128 | 4 | 5 | 404 | 2 | 405 | 7.61e-178 | 503 |
| MS.gene012473.t1 | AT4G23920 | 29.851 | 335 | 185 | 13 | 96 | 403 | 4 | 315 | 1.24e-22 | 98.6 |
| MS.gene012473.t1 | AT3G14790 | 31.330 | 233 | 150 | 5 | 96 | 324 | 8 | 234 | 3.99e-21 | 96.3 |
| MS.gene012473.t1 | AT3G14790 | 31.330 | 233 | 150 | 5 | 96 | 324 | 11 | 237 | 4.00e-21 | 96.3 |
| MS.gene012473.t1 | AT1G53500 | 30.901 | 233 | 151 | 5 | 96 | 324 | 10 | 236 | 1.50e-20 | 94.4 |
| MS.gene012473.t1 | AT1G78570 | 30.472 | 233 | 152 | 5 | 96 | 324 | 8 | 234 | 3.89e-20 | 93.2 |
| MS.gene012473.t1 | AT1G30620 | 25.602 | 332 | 214 | 10 | 90 | 403 | 65 | 381 | 2.59e-19 | 89.7 |
| MS.gene012473.t1 | AT1G30620 | 25.602 | 332 | 214 | 10 | 90 | 403 | 66 | 382 | 2.61e-19 | 89.7 |
| MS.gene012473.t1 | AT1G30620 | 25.602 | 332 | 214 | 10 | 90 | 403 | 66 | 382 | 2.61e-19 | 89.7 |
| MS.gene012473.t1 | AT1G30620 | 25.602 | 332 | 214 | 10 | 90 | 403 | 66 | 382 | 2.61e-19 | 89.7 |
| MS.gene012473.t1 | AT1G30620 | 25.602 | 332 | 214 | 10 | 90 | 403 | 66 | 382 | 2.61e-19 | 89.7 |
| MS.gene012473.t1 | AT4G10960 | 29.290 | 338 | 183 | 16 | 96 | 403 | 5 | 316 | 4.46e-19 | 88.2 |
| MS.gene012473.t1 | AT1G30620 | 29.744 | 195 | 133 | 3 | 90 | 282 | 66 | 258 | 1.42e-18 | 85.9 |
| MS.gene012473.t1 | AT5G44480 | 27.692 | 260 | 167 | 7 | 91 | 332 | 67 | 323 | 1.80e-18 | 87.0 |
| MS.gene012473.t1 | AT5G44480 | 27.692 | 260 | 167 | 7 | 91 | 332 | 67 | 323 | 1.80e-18 | 87.0 |
| MS.gene012473.t1 | AT5G44480 | 27.692 | 260 | 167 | 7 | 91 | 332 | 67 | 323 | 1.80e-18 | 87.0 |
| MS.gene012473.t1 | AT5G44480 | 27.692 | 260 | 167 | 7 | 91 | 332 | 91 | 347 | 2.09e-18 | 87.0 |
| MS.gene012473.t1 | AT1G12780 | 27.327 | 333 | 200 | 13 | 96 | 404 | 8 | 322 | 7.81e-18 | 84.7 |
| MS.gene012473.t1 | AT4G20460 | 27.969 | 261 | 165 | 8 | 91 | 332 | 66 | 322 | 1.53e-17 | 84.3 |
| MS.gene012473.t1 | AT2G28760 | 27.632 | 304 | 179 | 12 | 92 | 384 | 28 | 301 | 1.73e-17 | 83.6 |
| MS.gene012473.t1 | AT2G28760 | 27.632 | 304 | 179 | 12 | 92 | 384 | 28 | 301 | 1.73e-17 | 83.6 |
| MS.gene012473.t1 | AT2G28760 | 27.632 | 304 | 179 | 12 | 92 | 384 | 28 | 301 | 1.73e-17 | 83.6 |
| MS.gene012473.t1 | AT2G28760 | 27.632 | 304 | 179 | 12 | 92 | 384 | 59 | 332 | 2.28e-17 | 83.6 |
| MS.gene012473.t1 | AT3G62830 | 26.708 | 322 | 195 | 12 | 91 | 404 | 115 | 403 | 2.57e-17 | 84.0 |
| MS.gene012473.t1 | AT3G62830 | 26.708 | 322 | 195 | 12 | 91 | 404 | 115 | 403 | 2.57e-17 | 84.0 |
| MS.gene012473.t1 | AT1G63180 | 27.879 | 330 | 202 | 13 | 96 | 404 | 8 | 322 | 6.57e-17 | 82.0 |
| MS.gene012473.t1 | AT1G64440 | 26.426 | 333 | 199 | 11 | 96 | 403 | 4 | 315 | 7.86e-17 | 81.6 |
| MS.gene012473.t1 | AT2G47650 | 26.171 | 363 | 204 | 15 | 65 | 404 | 84 | 405 | 9.62e-17 | 82.0 |
| MS.gene012473.t1 | AT3G53520 | 26.246 | 301 | 180 | 11 | 95 | 384 | 120 | 389 | 2.25e-16 | 80.9 |
| MS.gene012473.t1 | AT1G63180 | 28.231 | 294 | 180 | 11 | 96 | 370 | 8 | 289 | 3.60e-16 | 79.0 |
| MS.gene012473.t1 | AT3G53520 | 27.731 | 238 | 148 | 8 | 95 | 325 | 120 | 340 | 5.42e-15 | 76.3 |
| MS.gene012473.t1 | AT2G47650 | 25.994 | 327 | 189 | 14 | 95 | 404 | 121 | 411 | 7.46e-15 | 76.3 |
| MS.gene012473.t1 | AT3G53520 | 25.914 | 301 | 179 | 12 | 95 | 384 | 120 | 387 | 4.27e-14 | 73.9 |
| MS.gene012473.t1 | AT3G53520 | 27.572 | 243 | 150 | 9 | 95 | 330 | 120 | 343 | 2.40e-13 | 72.0 |
Find 108 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTTCAAGTTCGGTTTA+TGG | 0.210656 | 8.3:-1817922 | MS.gene012473:CDS |
| ACCGGCGCAGCTGGTTTCAT+TGG | 0.250792 | 8.3:-1818279 | MS.gene012473:CDS |
| ATCCTCAACCGTCGATTGTT+TGG | 0.264214 | 8.3:-1817945 | MS.gene012473:CDS |
| CACACTTATAATCACATTTA+CGG | 0.269492 | 8.3:-1817805 | MS.gene012473:CDS |
| TCCTCAACCGTCGATTGTTT+GGG | 0.276207 | 8.3:-1817944 | MS.gene012473:CDS |
| GCCGGTATCTCTCCGCTTCA+TGG | 0.283731 | 8.3:-1818377 | MS.gene012473:CDS |
| CGTCAGGTTCACCGTTGTTT+CGG | 0.306919 | 8.3:-1818489 | MS.gene012473:CDS |
| CGGTTTATCGGGGCAAGAAT+CGG | 0.310974 | 8.3:-1817675 | MS.gene012473:CDS |
| TTTCACTAGAAATATGCTTC+AGG | 0.320164 | 8.3:-1817710 | MS.gene012473:CDS |
| AATGCCATCATCATCATCTT+TGG | 0.333111 | 8.3:-1818577 | MS.gene012473:CDS |
| TTTCAAGGCGTCGTTGATTC+CGG | 0.335632 | 8.3:-1818402 | MS.gene012473:CDS |
| TCTTCGAGCCGAAGTGATAT+TGG | 0.337799 | 8.3:+1817396 | None:intergenic |
| GTCTTTCAATCACTGGTTTA+CGG | 0.353235 | 8.3:-1817783 | MS.gene012473:CDS |
| ACCGTATCGGATCTTTAATC+TGG | 0.354061 | 8.3:-1817539 | MS.gene012473:CDS |
| ACTTCGGCTCGAAGAGAATT+CGG | 0.356084 | 8.3:-1817388 | MS.gene012473:CDS |
| TTCAAGCACCATGTTCTTGT+GGG | 0.364536 | 8.3:-1818463 | MS.gene012473:CDS |
| GGTTTATCGGGGCAAGAATC+GGG | 0.379457 | 8.3:-1817674 | MS.gene012473:CDS |
| TTGCCCCGATAAACCGTAAT+CGG | 0.384988 | 8.3:+1817682 | None:intergenic |
| CCGGCGCAGCTGGTTTCATT+GGG | 0.399259 | 8.3:-1818278 | MS.gene012473:CDS |
| GTTGGATCATTGGATACTTC+GGG | 0.400108 | 8.3:-1817601 | MS.gene012473:CDS |
| GCTTCAGGGAAAACCGATTA+CGG | 0.407580 | 8.3:-1817695 | MS.gene012473:CDS |
| TGTTTGGGCTTCTTCAAGTT+CGG | 0.422515 | 8.3:-1817929 | MS.gene012473:CDS |
| GAAAACCGATTACGGTTTAT+CGG | 0.422806 | 8.3:-1817687 | MS.gene012473:CDS |
| CCGGTATCTCTCCGCTTCAT+GGG | 0.427134 | 8.3:-1818376 | MS.gene012473:CDS |
| CTTCAAGCACCATGTTCTTG+TGG | 0.437354 | 8.3:-1818464 | MS.gene012473:CDS |
| TTCACTAGAAATATGCTTCA+GGG | 0.439425 | 8.3:-1817709 | MS.gene012473:CDS |
| GAAGGGATGTGTTGGATCAT+TGG | 0.440266 | 8.3:-1817611 | MS.gene012473:CDS |
| GTACTTGTCACCGGCGCAGC+TGG | 0.442621 | 8.3:-1818288 | MS.gene012473:CDS |
| AAAACCGATTACGGTTTATC+GGG | 0.446835 | 8.3:-1817686 | MS.gene012473:CDS |
| CTTCGGCTCGAAGAGAATTC+GGG | 0.447409 | 8.3:-1817387 | MS.gene012473:intron |
| CTTCATGGGGTGGAATTCAA+TGG | 0.449305 | 8.3:-1818362 | MS.gene012473:CDS |
| ATCGTAGATATGCCTGGAAA+TGG | 0.451456 | 8.3:-1817439 | MS.gene012473:CDS |
| CCCATGAAGCGGAGAGATAC+CGG | 0.457700 | 8.3:+1818376 | None:intergenic |
| ATCGGATCGGACCGATCAAC+CGG | 0.460933 | 8.3:-1817872 | MS.gene012473:CDS |
| CCCAATGAAACCAGCTGCGC+CGG | 0.461392 | 8.3:+1818278 | None:intergenic |
| TGTTGGATCATTGGATACTT+CGG | 0.470042 | 8.3:-1817602 | MS.gene012473:CDS |
| GTACTGACATGCCGCCTTGT+CGG | 0.473888 | 8.3:+1818307 | None:intergenic |
| CAGTTGATGCAATAAGCAAA+AGG | 0.478113 | 8.3:+1818439 | None:intergenic |
| GTCCGATCCGATTCAGAAAA+CGG | 0.482746 | 8.3:+1817883 | None:intergenic |
| ATTTACGGTCTTTCAATCAC+TGG | 0.485594 | 8.3:-1817790 | MS.gene012473:CDS |
| GCCGGTGACGGTGCCGACAC+TGG | 0.488782 | 8.3:-1817506 | MS.gene012473:CDS |
| GATACTTCGGGTAAGAGTAC+TGG | 0.490010 | 8.3:-1817589 | MS.gene012473:CDS |
| AAGAATATCGTAGATATGCC+TGG | 0.494476 | 8.3:-1817445 | MS.gene012473:CDS |
| AAGGGAACATCACCATTTCC+AGG | 0.495063 | 8.3:+1817427 | None:intergenic |
| ACCAGTGTCGGCACCGTCAC+CGG | 0.499934 | 8.3:+1817505 | None:intergenic |
| TTTCACTGTTTATGGACCAT+GGG | 0.501706 | 8.3:-1817758 | MS.gene012473:CDS |
| ATATAAACTCGCCGGTTGAT+CGG | 0.504741 | 8.3:+1817861 | None:intergenic |
| TTCATGGGGTGGAATTCAAT+GGG | 0.507931 | 8.3:-1818361 | MS.gene012473:CDS |
| TCTCTTGCTTTGAAAAGAAG+AGG | 0.508211 | 8.3:-1818246 | MS.gene012473:CDS |
| CCGTATCGGATCTTTAATCT+GGG | 0.514097 | 8.3:-1817538 | MS.gene012473:CDS |
| AACGTGGAGCAGCACCGTAT+CGG | 0.516784 | 8.3:-1817552 | MS.gene012473:CDS |
| AGCGGAGAGATACCGGCTAC+CGG | 0.518013 | 8.3:+1818383 | None:intergenic |
| GGGGAATACTTCGCCGGTGA+CGG | 0.520167 | 8.3:-1817518 | MS.gene012473:CDS |
| CTTCACTATATCGTCAATGT+AGG | 0.520270 | 8.3:+1817630 | None:intergenic |
| AGAAGAGGTGATGGTGTCGT+TGG | 0.521236 | 8.3:-1818231 | MS.gene012473:CDS |
| CTGGAGTGGATGGGTAGAGT+TGG | 0.525095 | 8.3:+1818550 | None:intergenic |
| TAATCTGGGGAATACTTCGC+CGG | 0.527767 | 8.3:-1817524 | MS.gene012473:CDS |
| TCTTCTTGAAGCTTGCAAAA+CGG | 0.530117 | 8.3:-1817971 | MS.gene012473:CDS |
| GTGATATTGGCATGGGTGAA+GGG | 0.530157 | 8.3:+1817409 | None:intergenic |
| AGCTGGTGTCAGATACGCTA+TGG | 0.534008 | 8.3:-1818040 | MS.gene012473:CDS |
| TTGCTGTTAACATATGACAT+AGG | 0.539948 | 8.3:+1818012 | None:intergenic |
| CAACTCTACCCATCCACTCC+AGG | 0.542396 | 8.3:-1818549 | MS.gene012473:CDS |
| TTCAAGAAGAGTGACTAAAC+CGG | 0.543596 | 8.3:+1817984 | None:intergenic |
| TATCTCTCCGCTTCATGGGG+TGG | 0.546092 | 8.3:-1818372 | MS.gene012473:CDS |
| GCCCAAACAATCGACGGTTG+AGG | 0.547804 | 8.3:+1817943 | None:intergenic |
| AGTGATATTGGCATGGGTGA+AGG | 0.548496 | 8.3:+1817408 | None:intergenic |
| GCGTCGTTGATTCCGGTAGC+CGG | 0.549486 | 8.3:-1818395 | MS.gene012473:CDS |
| GATATAGTGAAGGGATGTGT+TGG | 0.556067 | 8.3:-1817619 | MS.gene012473:CDS |
| TTTCAAAGCAAGAGAAACAT+GGG | 0.556424 | 8.3:+1818254 | None:intergenic |
| CCACGGTCTAATGAACCGTC+AGG | 0.559363 | 8.3:-1818505 | MS.gene012473:CDS |
| GCCATGTCTGGTCTTCCCCA+TGG | 0.560372 | 8.3:+1817742 | None:intergenic |
| TCGGGTAAGAGTACTGGATC+GGG | 0.560620 | 8.3:-1817583 | MS.gene012473:CDS |
| AGTTGATGCAATAAGCAAAA+GGG | 0.561287 | 8.3:+1818440 | None:intergenic |
| TTCGGGTAAGAGTACTGGAT+CGG | 0.563210 | 8.3:-1817584 | MS.gene012473:CDS |
| GGTTTATGGTTTAAACGAGA+AGG | 0.563313 | 8.3:-1817908 | MS.gene012473:CDS |
| ATGCATCTCGCAGCTCAAGC+TGG | 0.567280 | 8.3:-1818057 | MS.gene012473:CDS |
| TATGCAGCGACGAAGAAAGC+AGG | 0.574218 | 8.3:-1817841 | MS.gene012473:CDS |
| CAAAAGGGCCCACAAGAACA+TGG | 0.581018 | 8.3:+1818455 | None:intergenic |
| GAGCCGAAGTGATATTGGCA+TGG | 0.582998 | 8.3:+1817401 | None:intergenic |
| GTCGCTGCATATAAACTCGC+CGG | 0.586523 | 8.3:+1817853 | None:intergenic |
| TTGTCGGTGGATTTGAGCGG+AGG | 0.587662 | 8.3:+1818323 | None:intergenic |
| CACCCATGCCAATATCACTT+CGG | 0.588902 | 8.3:-1817404 | MS.gene012473:CDS |
| AGCCGAAGTGATATTGGCAT+GGG | 0.591042 | 8.3:+1817402 | None:intergenic |
| CTGACATGCCGCCTTGTCGG+TGG | 0.593746 | 8.3:+1818310 | None:intergenic |
| CCCAGATTAAAGATCCGATA+CGG | 0.595228 | 8.3:+1817538 | None:intergenic |
| CGAAACAACGGTGAACCTGA+CGG | 0.595789 | 8.3:+1818490 | None:intergenic |
| TATGTTAACAGCAACATCGC+CGG | 0.597339 | 8.3:-1818003 | MS.gene012473:CDS |
| CGGTATCTCTCCGCTTCATG+GGG | 0.598560 | 8.3:-1818375 | MS.gene012473:CDS |
| TTGAATTCCACCCCATGAAG+CGG | 0.604390 | 8.3:+1818365 | None:intergenic |
| CTACATTGACGATATAGTGA+AGG | 0.605438 | 8.3:-1817629 | MS.gene012473:CDS |
| TCCGCTCAAATCCACCGACA+AGG | 0.624212 | 8.3:-1818321 | MS.gene012473:CDS |
| GGCATGTCAGTACTTGTCAC+CGG | 0.625199 | 8.3:-1818297 | MS.gene012473:CDS |
| TACATTGACGATATAGTGAA+GGG | 0.627843 | 8.3:-1817628 | MS.gene012473:CDS |
| GGTAAGAGTACTGGATCGGG+TGG | 0.630428 | 8.3:-1817580 | MS.gene012473:CDS |
| GCCTTGTCGGTGGATTTGAG+CGG | 0.637649 | 8.3:+1818320 | None:intergenic |
| GCTTTGAAAAGAAGAGGTGA+TGG | 0.645226 | 8.3:-1818240 | MS.gene012473:CDS |
| TTCAAGATCGAAAGAAACCA+CGG | 0.649323 | 8.3:-1818522 | MS.gene012473:CDS |
| ACCATGGGGAAGACCAGACA+TGG | 0.649667 | 8.3:-1817743 | MS.gene012473:CDS |
| TTCACTGTTTATGGACCATG+GGG | 0.649938 | 8.3:-1817757 | MS.gene012473:CDS |
| TCTTCCAAAGATGATGATGA+TGG | 0.654769 | 8.3:+1818573 | None:intergenic |
| TGCTTGAAGAACCGAAACAA+CGG | 0.657956 | 8.3:+1818478 | None:intergenic |
| TCAAGTATACTCACCAGTGT+CGG | 0.663697 | 8.3:+1817493 | None:intergenic |
| AAACCGATTACGGTTTATCG+GGG | 0.665354 | 8.3:-1817685 | MS.gene012473:CDS |
| GAAGAAGCCCAAACAATCGA+CGG | 0.665669 | 8.3:+1817937 | None:intergenic |
| CGTATCGGATCTTTAATCTG+GGG | 0.681906 | 8.3:-1817537 | MS.gene012473:CDS |
| GGATCGGGTGGAAAGAAACG+TGG | 0.697082 | 8.3:-1817568 | MS.gene012473:CDS |
| CCTGACGGTTCATTAGACCG+TGG | 0.701311 | 8.3:+1818505 | None:intergenic |
| GCTCAAATCCACCGACAAGG+CGG | 0.711632 | 8.3:-1818318 | MS.gene012473:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTCTTTCTTAGATTTT+AGG | - | chr8.3:1818357-1818376 | MS.gene012473:CDS | 15.0% |
| !! | TATCTTTCTTATTATGGTTA+TGG | - | chr8.3:1818305-1818324 | MS.gene012473:CDS | 20.0% |
| !!! | ATATATTCTTTCCTTCTTTT+TGG | + | chr8.3:1818438-1818457 | None:intergenic | 20.0% |
| !!! | GATTTTAATTTACAGTGTTA+TGG | - | chr8.3:1818379-1818398 | MS.gene012473:CDS | 20.0% |
| ! | AAGTGGTATCTTTCTTATTA+TGG | - | chr8.3:1818299-1818318 | MS.gene012473:CDS | 25.0% |
| ! | ATTATTCTTTACTTGAGAAG+AGG | - | chr8.3:1818466-1818485 | MS.gene012473:CDS | 25.0% |
| ! | CACACTTATAATCACATTTA+CGG | - | chr8.3:1817813-1817832 | MS.gene012473:CDS | 25.0% |
| ! | GAAATACTAAACCAGAAAAA+AGG | - | chr8.3:1818404-1818423 | MS.gene012473:CDS | 25.0% |
| !! | TTTAATTTACAGTGTTATGG+AGG | - | chr8.3:1818382-1818401 | MS.gene012473:CDS | 25.0% |
| AAAGTAAAAATAGCCATGTC+TGG | + | chr8.3:1817891-1817910 | None:intergenic | 30.0% | |
| AGTAGAAAAGAAATCTGTCA+AGG | + | chr8.3:1818520-1818539 | None:intergenic | 30.0% | |
| TACATTGACGATATAGTGAA+GGG | - | chr8.3:1817990-1818009 | MS.gene012473:CDS | 30.0% | |
| TTCACTAGAAATATGCTTCA+GGG | - | chr8.3:1817909-1817928 | MS.gene012473:CDS | 30.0% | |
| TTTCAAAGCAAGAGAAACAT+GGG | + | chr8.3:1817367-1817386 | None:intergenic | 30.0% | |
| TTTCACTAGAAATATGCTTC+AGG | - | chr8.3:1817908-1817927 | MS.gene012473:CDS | 30.0% | |
| ! | AGTTGATGCAATAAGCAAAA+GGG | + | chr8.3:1817181-1817200 | None:intergenic | 30.0% |
| ! | TTACGGTTTTTCACTGTTTA+TGG | - | chr8.3:1817852-1817871 | MS.gene012473:CDS | 30.0% |
| ! | TTGCTGTTAACATATGACAT+AGG | + | chr8.3:1817609-1817628 | None:intergenic | 30.0% |
| ! | TTTTCAAAGCAAGAGAAACA+TGG | + | chr8.3:1817368-1817387 | None:intergenic | 30.0% |
| !!! | GTTTCTTATGTCACTTTTCA+AGG | - | chr8.3:1817201-1817220 | MS.gene012473:intron | 30.0% |
| !!! | TTTCGAGCTTTTTTCAAAGA+AGG | + | chr8.3:1817441-1817460 | None:intergenic | 30.0% |
| AAAACCGATTACGGTTTATC+GGG | - | chr8.3:1817932-1817951 | MS.gene012473:CDS | 35.0% | |
| AAGAATATCGTAGATATGCC+TGG | - | chr8.3:1818173-1818192 | MS.gene012473:CDS | 35.0% | |
| AGGAAAGAAGTCCAAAAAGA+AGG | - | chr8.3:1818424-1818443 | MS.gene012473:CDS | 35.0% | |
| ATTTACGGTCTTTCAATCAC+TGG | - | chr8.3:1817828-1817847 | MS.gene012473:CDS | 35.0% | |
| CTACATTGACGATATAGTGA+AGG | - | chr8.3:1817989-1818008 | MS.gene012473:CDS | 35.0% | |
| CTTCACTATATCGTCAATGT+AGG | + | chr8.3:1817991-1818010 | None:intergenic | 35.0% | |
| GAAAACCGATTACGGTTTAT+CGG | - | chr8.3:1817931-1817950 | MS.gene012473:CDS | 35.0% | |
| GGTTGAAAAAATTCGTCAAG+TGG | - | chr8.3:1818282-1818301 | MS.gene012473:CDS | 35.0% | |
| GGTTTATGGTTTAAACGAGA+AGG | - | chr8.3:1817710-1817729 | MS.gene012473:CDS | 35.0% | |
| TCTTCTTGAAGCTTGCAAAA+CGG | - | chr8.3:1817647-1817666 | MS.gene012473:CDS | 35.0% | |
| TGTTGGATCATTGGATACTT+CGG | - | chr8.3:1818016-1818035 | MS.gene012473:CDS | 35.0% | |
| TTCAAGAAGAGTGACTAAAC+CGG | + | chr8.3:1817637-1817656 | None:intergenic | 35.0% | |
| TTCAAGATCGAAAGAAACCA+CGG | - | chr8.3:1817096-1817115 | MS.gene012473:intron | 35.0% | |
| TTTCACTGTTTATGGACCAT+GGG | - | chr8.3:1817860-1817879 | MS.gene012473:CDS | 35.0% | |
| ! | CAGTTGATGCAATAAGCAAA+AGG | + | chr8.3:1817182-1817201 | None:intergenic | 35.0% |
| ! | CTTTCGATCTTGAATTTTCC+TGG | + | chr8.3:1817090-1817109 | None:intergenic | 35.0% |
| ! | TATACTTGAGCGACTTTTGA+AGG | - | chr8.3:1818139-1818158 | MS.gene012473:CDS | 35.0% |
| ! | TCTTCCAAAGATGATGATGA+TGG | + | chr8.3:1817048-1817067 | None:intergenic | 35.0% |
| ! | TTTTCACTGTTTATGGACCA+TGG | - | chr8.3:1817859-1817878 | MS.gene012473:CDS | 35.0% |
| !! | GTCTTTCAATCACTGGTTTA+CGG | - | chr8.3:1817835-1817854 | MS.gene012473:CDS | 35.0% |
| !! | TCTCTTGCTTTGAAAAGAAG+AGG | - | chr8.3:1817372-1817391 | MS.gene012473:intron | 35.0% |
| !!! | CTTGACGAATTTTTTCAACC+CGG | + | chr8.3:1818282-1818301 | None:intergenic | 35.0% |
| !!! | TGGACTTCTTTCCTTTTTTC+TGG | + | chr8.3:1818418-1818437 | None:intergenic | 35.0% |
| AAACCGATTACGGTTTATCG+GGG | - | chr8.3:1817933-1817952 | MS.gene012473:CDS | 40.0% | |
| ACCGTATCGGATCTTTAATC+TGG | - | chr8.3:1818079-1818098 | MS.gene012473:CDS | 40.0% | |
| ATCGTAGATATGCCTGGAAA+TGG | - | chr8.3:1818179-1818198 | MS.gene012473:CDS | 40.0% | |
| CCACTACTGATATTCAAACC+GGG | - | chr8.3:1818261-1818280 | MS.gene012473:CDS | 40.0% | |
| CCCACTACTGATATTCAAAC+CGG | - | chr8.3:1818260-1818279 | MS.gene012473:CDS | 40.0% | |
| CCCAGATTAAAGATCCGATA+CGG | + | chr8.3:1818083-1818102 | None:intergenic | 40.0% | |
| CCGTATCGGATCTTTAATCT+GGG | - | chr8.3:1818080-1818099 | MS.gene012473:CDS | 40.0% | |
| CGTATCGGATCTTTAATCTG+GGG | - | chr8.3:1818081-1818100 | MS.gene012473:CDS | 40.0% | |
| GATATAGTGAAGGGATGTGT+TGG | - | chr8.3:1817999-1818018 | MS.gene012473:CDS | 40.0% | |
| GTTGGATCATTGGATACTTC+GGG | - | chr8.3:1818017-1818036 | MS.gene012473:CDS | 40.0% | |
| TATGTTAACAGCAACATCGC+CGG | - | chr8.3:1817615-1817634 | MS.gene012473:CDS | 40.0% | |
| TCAAGTATACTCACCAGTGT+CGG | + | chr8.3:1818128-1818147 | None:intergenic | 40.0% | |
| TGCTTGAAGAACCGAAACAA+CGG | + | chr8.3:1817143-1817162 | None:intergenic | 40.0% | |
| TTCAAGCACCATGTTCTTGT+GGG | - | chr8.3:1817155-1817174 | MS.gene012473:intron | 40.0% | |
| TTCACTGTTTATGGACCATG+GGG | - | chr8.3:1817861-1817880 | MS.gene012473:CDS | 40.0% | |
| TTCATGGGGTGGAATTCAAT+GGG | - | chr8.3:1817257-1817276 | MS.gene012473:intron | 40.0% | |
| ! | AGCAAAGCTTTTCGATGTTG+TGG | - | chr8.3:1817524-1817543 | MS.gene012473:CDS | 40.0% |
| ! | ATATAAACTCGCCGGTTGAT+CGG | + | chr8.3:1817760-1817779 | None:intergenic | 40.0% |
| ! | CCGGTTTGAATATCAGTAGT+GGG | + | chr8.3:1818263-1818282 | None:intergenic | 40.0% |
| ! | GAAGGTTCCGTTTTCTGAAT+CGG | - | chr8.3:1817728-1817747 | MS.gene012473:CDS | 40.0% |
| ! | GATCTTGAATTTTCCTGGAG+TGG | + | chr8.3:1817085-1817104 | None:intergenic | 40.0% |
| ! | GCTTCTTCAAGTTCGGTTTA+TGG | - | chr8.3:1817696-1817715 | MS.gene012473:CDS | 40.0% |
| ! | TGAATTTTCCTGGAGTGGAT+GGG | + | chr8.3:1817080-1817099 | None:intergenic | 40.0% |
| ! | TGTTTGGGCTTCTTCAAGTT+CGG | - | chr8.3:1817689-1817708 | MS.gene012473:CDS | 40.0% |
| ! | TTCCGTTTTCTGAATCGGAT+CGG | - | chr8.3:1817733-1817752 | MS.gene012473:CDS | 40.0% |
| ! | TTGAATTTTCCTGGAGTGGA+TGG | + | chr8.3:1817081-1817100 | None:intergenic | 40.0% |
| !! | AAAGCGCTTTTATCATCTCG+CGG | - | chr8.3:1817459-1817478 | MS.gene012473:CDS | 40.0% |
| !! | GCTTTGAAAAGAAGAGGTGA+TGG | - | chr8.3:1817378-1817397 | MS.gene012473:intron | 40.0% |
| AAGGGAACATCACCATTTCC+AGG | + | chr8.3:1818194-1818213 | None:intergenic | 45.0% | |
| AGCCGAAGTGATATTGGCAT+GGG | + | chr8.3:1818219-1818238 | None:intergenic | 45.0% | |
| AGTGATATTGGCATGGGTGA+AGG | + | chr8.3:1818213-1818232 | None:intergenic | 45.0% | |
| CACCCATGCCAATATCACTT+CGG | - | chr8.3:1818214-1818233 | MS.gene012473:CDS | 45.0% | |
| CTTCAAGCACCATGTTCTTG+TGG | - | chr8.3:1817154-1817173 | MS.gene012473:intron | 45.0% | |
| CTTCATGGGGTGGAATTCAA+TGG | - | chr8.3:1817256-1817275 | MS.gene012473:intron | 45.0% | |
| GAAGAAGCCCAAACAATCGA+CGG | + | chr8.3:1817684-1817703 | None:intergenic | 45.0% | |
| GAAGGGATGTGTTGGATCAT+TGG | - | chr8.3:1818007-1818026 | MS.gene012473:CDS | 45.0% | |
| GCTTCAGGGAAAACCGATTA+CGG | - | chr8.3:1817923-1817942 | MS.gene012473:CDS | 45.0% | |
| GTCCGATCCGATTCAGAAAA+CGG | + | chr8.3:1817738-1817757 | None:intergenic | 45.0% | |
| GTGATATTGGCATGGGTGAA+GGG | + | chr8.3:1818212-1818231 | None:intergenic | 45.0% | |
| TAATCTGGGGAATACTTCGC+CGG | - | chr8.3:1818094-1818113 | MS.gene012473:CDS | 45.0% | |
| TCTTCGAGCCGAAGTGATAT+TGG | + | chr8.3:1818225-1818244 | None:intergenic | 45.0% | |
| TTGAATTCCACCCCATGAAG+CGG | + | chr8.3:1817256-1817275 | None:intergenic | 45.0% | |
| TTGCCCCGATAAACCGTAAT+CGG | + | chr8.3:1817939-1817958 | None:intergenic | 45.0% | |
| ! | ACTTCGGCTCGAAGAGAATT+CGG | - | chr8.3:1818230-1818249 | MS.gene012473:CDS | 45.0% |
| ! | ATCCTCAACCGTCGATTGTT+TGG | - | chr8.3:1817673-1817692 | MS.gene012473:CDS | 45.0% |
| ! | CCCGGTTTGAATATCAGTAG+TGG | + | chr8.3:1818264-1818283 | None:intergenic | 45.0% |
| ! | GATACTTCGGGTAAGAGTAC+TGG | - | chr8.3:1818029-1818048 | MS.gene012473:CDS | 45.0% |
| ! | TCCTCAACCGTCGATTGTTT+GGG | - | chr8.3:1817674-1817693 | MS.gene012473:CDS | 45.0% |
| !! | TGAGCGACTTTTGAAGGTGA+AGG | - | chr8.3:1818145-1818164 | MS.gene012473:CDS | 45.0% |
| !! | TTCGGGTAAGAGTACTGGAT+CGG | - | chr8.3:1818034-1818053 | MS.gene012473:CDS | 45.0% |
| !! | TTTCAAGGCGTCGTTGATTC+CGG | - | chr8.3:1817216-1817235 | MS.gene012473:intron | 45.0% |
| CAAAAGGGCCCACAAGAACA+TGG | + | chr8.3:1817166-1817185 | None:intergenic | 50.0% | |
| CGAAACAACGGTGAACCTGA+CGG | + | chr8.3:1817131-1817150 | None:intergenic | 50.0% | |
| CGGTTTATCGGGGCAAGAAT+CGG | - | chr8.3:1817943-1817962 | MS.gene012473:CDS | 50.0% | |
| CGTCAGGTTCACCGTTGTTT+CGG | - | chr8.3:1817129-1817148 | MS.gene012473:intron | 50.0% | |
| GAGCCGAAGTGATATTGGCA+TGG | + | chr8.3:1818220-1818239 | None:intergenic | 50.0% | |
| GGCATGTCAGTACTTGTCAC+CGG | - | chr8.3:1817321-1817340 | MS.gene012473:intron | 50.0% | |
| GGTTTATCGGGGCAAGAATC+GGG | - | chr8.3:1817944-1817963 | MS.gene012473:CDS | 50.0% | |
| GTCGCTGCATATAAACTCGC+CGG | + | chr8.3:1817768-1817787 | None:intergenic | 50.0% | |
| TATGCAGCGACGAAGAAAGC+AGG | - | chr8.3:1817777-1817796 | MS.gene012473:CDS | 50.0% | |
| ! | AGCTGGTGTCAGATACGCTA+TGG | - | chr8.3:1817578-1817597 | MS.gene012473:CDS | 50.0% |
| ! | CTTCGGCTCGAAGAGAATTC+GGG | - | chr8.3:1818231-1818250 | MS.gene012473:CDS | 50.0% |
| !! | AGAAGAGGTGATGGTGTCGT+TGG | - | chr8.3:1817387-1817406 | MS.gene012473:intron | 50.0% |
| !! | TCGGGTAAGAGTACTGGATC+GGG | - | chr8.3:1818035-1818054 | MS.gene012473:CDS | 50.0% |
| AACGTGGAGCAGCACCGTAT+CGG | - | chr8.3:1818066-1818085 | MS.gene012473:CDS | 55.0% | |
| ACCATGGGGAAGACCAGACA+TGG | - | chr8.3:1817875-1817894 | MS.gene012473:CDS | 55.0% | |
| ATCGGATCGGACCGATCAAC+CGG | - | chr8.3:1817746-1817765 | MS.gene012473:CDS | 55.0% | |
| ATGCATCTCGCAGCTCAAGC+TGG | - | chr8.3:1817561-1817580 | MS.gene012473:CDS | 55.0% | |
| CAACTCTACCCATCCACTCC+AGG | - | chr8.3:1817069-1817088 | MS.gene012473:intron | 55.0% | |
| CCACGGTCTAATGAACCGTC+AGG | - | chr8.3:1817113-1817132 | MS.gene012473:intron | 55.0% | |
| CCCATGAAGCGGAGAGATAC+CGG | + | chr8.3:1817245-1817264 | None:intergenic | 55.0% | |
| CCGGTATCTCTCCGCTTCAT+GGG | - | chr8.3:1817242-1817261 | MS.gene012473:intron | 55.0% | |
| CCTGACGGTTCATTAGACCG+TGG | + | chr8.3:1817116-1817135 | None:intergenic | 55.0% | |
| CGGTATCTCTCCGCTTCATG+GGG | - | chr8.3:1817243-1817262 | MS.gene012473:intron | 55.0% | |
| CTGGAGTGGATGGGTAGAGT+TGG | + | chr8.3:1817071-1817090 | None:intergenic | 55.0% | |
| GCCCAAACAATCGACGGTTG+AGG | + | chr8.3:1817678-1817697 | None:intergenic | 55.0% | |
| GCTCAAATCCACCGACAAGG+CGG | - | chr8.3:1817300-1817319 | MS.gene012473:intron | 55.0% | |
| GGATCGGGTGGAAAGAAACG+TGG | - | chr8.3:1818050-1818069 | MS.gene012473:CDS | 55.0% | |
| TATCTCTCCGCTTCATGGGG+TGG | - | chr8.3:1817246-1817265 | MS.gene012473:intron | 55.0% | |
| TCCGCTCAAATCCACCGACA+AGG | - | chr8.3:1817297-1817316 | MS.gene012473:intron | 55.0% | |
| ! | GCCTTGTCGGTGGATTTGAG+CGG | + | chr8.3:1817301-1817320 | None:intergenic | 55.0% |
| ! | GTACTGACATGCCGCCTTGT+CGG | + | chr8.3:1817314-1817333 | None:intergenic | 55.0% |
| ! | TTGTCGGTGGATTTGAGCGG+AGG | + | chr8.3:1817298-1817317 | None:intergenic | 55.0% |
| !! | GGTAAGAGTACTGGATCGGG+TGG | - | chr8.3:1818038-1818057 | MS.gene012473:CDS | 55.0% |
| CCCAATGAAACCAGCTGCGC+CGG | + | chr8.3:1817343-1817362 | None:intergenic | 60.0% | |
| GCCGGTATCTCTCCGCTTCA+TGG | - | chr8.3:1817241-1817260 | MS.gene012473:intron | 60.0% | |
| GGGGAATACTTCGCCGGTGA+CGG | - | chr8.3:1818100-1818119 | MS.gene012473:CDS | 60.0% | |
| ! | ACCGGCGCAGCTGGTTTCAT+TGG | - | chr8.3:1817339-1817358 | MS.gene012473:intron | 60.0% |
| ! | AGCGGAGAGATACCGGCTAC+CGG | + | chr8.3:1817238-1817257 | None:intergenic | 60.0% |
| ! | CCGGCGCAGCTGGTTTCATT+GGG | - | chr8.3:1817340-1817359 | MS.gene012473:intron | 60.0% |
| ! | CGCGGCGTTTTCATCGTGCA+TGG | - | chr8.3:1817477-1817496 | MS.gene012473:CDS | 60.0% |
| ! | GCCATGTCTGGTCTTCCCCA+TGG | + | chr8.3:1817879-1817898 | None:intergenic | 60.0% |
| !! | GCGTCGTTGATTCCGGTAGC+CGG | - | chr8.3:1817223-1817242 | MS.gene012473:intron | 60.0% |
| CTGACATGCCGCCTTGTCGG+TGG | + | chr8.3:1817311-1817330 | None:intergenic | 65.0% | |
| GTACTTGTCACCGGCGCAGC+TGG | - | chr8.3:1817330-1817349 | MS.gene012473:intron | 65.0% | |
| !! | ACCAGTGTCGGCACCGTCAC+CGG | + | chr8.3:1818116-1818135 | None:intergenic | 65.0% |
| !! | GCCGGTGACGGTGCCGACAC+TGG | - | chr8.3:1818112-1818131 | MS.gene012473:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 1817042 | 1818598 | 1817042 | ID=MS.gene012473 |
| chr8.3 | mRNA | 1817042 | 1818598 | 1817042 | ID=MS.gene012473.t1;Parent=MS.gene012473 |
| chr8.3 | exon | 1817388 | 1818598 | 1817388 | ID=MS.gene012473.t1.exon1;Parent=MS.gene012473.t1 |
| chr8.3 | CDS | 1817388 | 1818598 | 1817388 | ID=cds.MS.gene012473.t1;Parent=MS.gene012473.t1 |
| chr8.3 | exon | 1817042 | 1817057 | 1817042 | ID=MS.gene012473.t1.exon2;Parent=MS.gene012473.t1 |
| chr8.3 | CDS | 1817042 | 1817057 | 1817042 | ID=cds.MS.gene012473.t1;Parent=MS.gene012473.t1 |
| Gene Sequence |
| Protein sequence |
