AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50266

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50266.t1 MTR_4g024600 99.773 440 1 0 1 440 1 440 0.0 907
MS.gene50266.t1 MTR_0354s0040 82.629 426 66 3 15 440 4 421 0.0 728
MS.gene50266.t1 MTR_4g102270 69.431 422 125 3 11 432 7 424 0.0 603
MS.gene50266.t1 MTR_4g129820 67.431 436 132 4 8 440 6 434 0.0 593
MS.gene50266.t1 MTR_3g084090 66.047 430 134 3 13 432 3 430 0.0 585
MS.gene50266.t1 MTR_5g011940 76.294 367 85 1 69 433 56 422 0.0 581
MS.gene50266.t1 MTR_0034s0060 27.545 334 213 8 102 428 138 449 9.11e-22 98.6
MS.gene50266.t1 MTR_8g096880 28.614 332 199 12 103 412 8 323 1.45e-21 95.9
MS.gene50266.t1 MTR_8g096880 29.042 334 195 13 103 412 8 323 2.08e-21 94.7
MS.gene50266.t1 MTR_2g073240 26.822 343 208 8 102 430 20 333 2.62e-20 94.0
MS.gene50266.t1 MTR_2g032100 26.087 345 219 12 103 427 73 401 2.65e-20 92.8
MS.gene50266.t1 MTR_5g009170 26.039 361 230 12 91 433 38 379 3.38e-20 92.4
MS.gene50266.t1 MTR_8g099535 27.576 330 205 12 102 412 5 319 7.92e-20 90.9
MS.gene50266.t1 MTR_1g009600 25.519 337 220 10 102 430 6 319 3.17e-19 90.9
MS.gene50266.t1 MTR_4g120030 25.397 441 251 17 8 425 20 405 6.54e-19 89.0
MS.gene50266.t1 MTR_5g009160 26.923 338 203 11 99 412 2 319 1.07e-18 87.4
MS.gene50266.t1 MTR_1g088480 25.816 337 202 12 98 425 115 412 1.20e-18 88.2
MS.gene50266.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene50266.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene50266.t1 MTR_4g063600 24.471 331 212 11 98 421 29 328 2.91e-17 83.2
MS.gene50266.t1 MTR_8g096880 28.041 296 176 11 103 376 8 288 8.39e-17 81.3
MS.gene50266.t1 MTR_4g063600 24.773 331 208 12 98 421 29 325 2.90e-16 80.1
MS.gene50266.t1 MTR_7g115080 25.000 336 210 13 103 427 31 335 2.48e-15 77.8
MS.gene50266.t1 MTR_7g115080 25.000 336 210 13 103 427 65 369 2.64e-15 77.8
MS.gene50266.t1 MTR_7g115080 25.000 336 210 13 103 427 44 348 2.70e-15 77.8
MS.gene50266.t1 MTR_1g080950 23.810 399 247 16 41 427 18 371 4.46e-15 77.0
MS.gene50266.t1 MTR_2g032100 29.630 189 127 5 103 288 73 258 6.25e-15 75.5
MS.gene50266.t1 MTR_1g080950 24.189 339 214 12 101 427 28 335 8.07e-15 76.3
MS.gene50266.t1 MTR_2g096660 24.324 333 210 12 98 421 35 334 1.67e-14 75.1
MS.gene50266.t1 MTR_4g101940 86.667 30 4 0 173 202 42 71 2.71e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50266.t1 AT4G00110 86.667 435 51 4 4 438 1 428 0.0 770
MS.gene50266.t1 AT1G02000 84.828 435 59 5 7 439 1 430 0.0 751
MS.gene50266.t1 AT2G45310 78.851 435 88 3 7 438 1 434 0.0 693
MS.gene50266.t1 AT4G12250 73.059 438 105 4 7 437 1 432 0.0 621
MS.gene50266.t1 AT3G23820 77.557 352 79 0 81 432 92 443 0.0 582
MS.gene50266.t1 AT4G30440 67.907 430 126 4 11 438 6 425 0.0 564
MS.gene50266.t1 AT4G23920 27.350 351 215 12 102 430 4 336 4.19e-23 100
MS.gene50266.t1 AT1G12780 28.657 335 197 13 102 412 8 324 1.10e-22 99.0
MS.gene50266.t1 AT1G63180 28.443 334 199 12 102 412 8 324 5.08e-21 94.4
MS.gene50266.t1 AT1G78570 26.647 334 216 8 102 428 8 319 8.81e-21 95.5
MS.gene50266.t1 AT3G14790 26.647 334 216 8 102 428 11 322 2.27e-20 94.4
MS.gene50266.t1 AT3G14790 26.647 334 216 8 102 428 8 319 2.54e-20 94.4
MS.gene50266.t1 AT4G10960 26.891 357 209 13 102 430 5 337 7.75e-20 90.9
MS.gene50266.t1 AT1G30620 25.507 345 221 11 103 427 72 400 4.10e-19 89.4
MS.gene50266.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene50266.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene50266.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene50266.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene50266.t1 AT1G53500 26.393 341 208 8 102 428 10 321 9.08e-19 89.4
MS.gene50266.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene50266.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene50266.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene50266.t1 AT5G44480 24.709 344 225 10 103 427 97 425 2.18e-18 87.4
MS.gene50266.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene50266.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene50266.t1 AT3G53520 25.294 340 215 10 98 430 117 424 1.40e-17 85.1
MS.gene50266.t1 AT1G64440 25.281 356 220 12 102 434 4 336 1.42e-17 84.0
MS.gene50266.t1 AT2G47650 24.122 427 252 16 32 426 35 421 3.98e-17 83.6
MS.gene50266.t1 AT4G20460 25.212 353 227 12 96 427 64 400 4.53e-17 83.2
MS.gene50266.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene50266.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene50266.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene50266.t1 AT2G28760 25.225 333 207 13 98 421 59 358 8.34e-17 82.0
MS.gene50266.t1 AT1G63180 28.188 298 175 11 102 376 8 289 9.29e-17 81.3
MS.gene50266.t1 AT3G53520 25.000 340 214 11 98 430 117 422 1.72e-15 78.6
MS.gene50266.t1 AT2G47650 24.249 433 250 18 32 426 35 427 3.98e-15 77.4
MS.gene50266.t1 AT5G59290 24.085 328 207 12 103 421 47 341 7.57e-15 76.3
MS.gene50266.t1 AT5G59290 24.085 328 207 12 103 421 32 326 8.39e-15 75.9
MS.gene50266.t1 AT5G59290 24.085 328 207 12 103 421 3 297 9.21e-15 75.5
MS.gene50266.t1 AT5G59290 24.085 328 207 12 103 421 25 319 1.03e-14 75.5
MS.gene50266.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene50266.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene50266.t1 AT3G53520 27.083 240 153 6 98 331 117 340 2.60e-14 74.3
MS.gene50266.t1 AT3G53520 24.011 354 225 12 98 430 117 447 3.55e-14 74.7
MS.gene50266.t1 AT1G30620 27.128 188 133 3 103 288 73 258 1.37e-13 71.6

Find 116 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGTTCTAGTTCTGTTTA+TGG 0.169613 4.4:+77086802 MS.gene50266:CDS
GTTCTATCCCTTTCAGAAAA+AGG 0.194999 4.4:-77086841 None:intergenic
ATCTATGGTCTTTCTTTAAC+TGG 0.228130 4.4:+77086934 MS.gene50266:CDS
ATCCTCAACCTTCAATTGTT+TGG 0.242672 4.4:+77086779 MS.gene50266:CDS
CTCCGGCGAAGCTTTGTCTT+TGG 0.247408 4.4:+77086239 MS.gene50266:CDS
TATCTGAATTACTATTCTTC+TGG 0.256882 4.4:+77087411 MS.gene50266:CDS
TAGTGATCTTGTGGGTATTT+TGG 0.296077 4.4:+77087230 MS.gene50266:CDS
TTCTCCATCTTGAACTTTCC+CGG 0.301644 4.4:-77086181 None:intergenic
TCCTCAACCTTCAATTGTTT+GGG 0.302294 4.4:+77086780 MS.gene50266:CDS
AAATGCATCACATGAGTAAA+AGG 0.305224 4.4:-77086652 None:intergenic
TTTCACTGTTTATGGTCCTT+GGG 0.318056 4.4:+77086966 MS.gene50266:CDS
TCCTTGGGGTAGGCCTGATA+TGG 0.329537 4.4:+77086981 MS.gene50266:CDS
CAAACTGGGGTGGTCCCGTT+TGG 0.334864 4.4:+77086362 MS.gene50266:CDS
GCGTGCAAGACAAGCACTTT+TGG 0.348677 4.4:+77086555 MS.gene50266:CDS
GTCTTTCTTTAACTGGTTTG+AGG 0.353381 4.4:+77086941 MS.gene50266:CDS
TATGTTCATAGTAACATTGC+TGG 0.365279 4.4:+77086721 MS.gene50266:CDS
CATTGAGGAGTCCTTCAAAC+TGG 0.372195 4.4:+77086347 MS.gene50266:CDS
CATACTTATAATCATATCTA+TGG 0.374841 4.4:+77086919 MS.gene50266:CDS
TAAACCGGTGACGGATCTTC+AGG 0.382536 4.4:+77087362 MS.gene50266:CDS
CTCTTCCGGCGGACCCTTCA+CGG 0.384471 4.4:+77086320 MS.gene50266:CDS
AAACTGGGGTGGTCCCGTTT+GGG 0.395396 4.4:+77086363 MS.gene50266:CDS
TTATGAAGTTGCCGAGGAAT+GGG 0.400306 4.4:+77087286 MS.gene50266:CDS
ATCTGAATTACTATTCTTCT+GGG 0.411188 4.4:+77087412 MS.gene50266:CDS
CTGTTTATGGTCCTTGGGGT+AGG 0.421551 4.4:+77086971 MS.gene50266:CDS
CTGGAAGTGGTGGGAAGAAG+AGG 0.431142 4.4:+77087154 MS.gene50266:CDS
TCAATGATGCTGCTTTGTTA+AGG 0.431669 4.4:+77086611 MS.gene50266:CDS
GTTCTTGTGACCGGAGCTGC+TGG 0.437389 4.4:+77086436 MS.gene50266:CDS
TCCGGCGACTTCTCCTCTTC+CGG 0.441112 4.4:+77086306 MS.gene50266:CDS
GGAGAGGCTGTTGAAGACTA+AGG 0.452325 4.4:+77087251 MS.gene50266:CDS
TGTACGGTGGCACGCTTCTC+CGG 0.452864 4.4:+77086222 MS.gene50266:CDS
GAACTAGCCCAAACAATTGA+AGG 0.455646 4.4:-77086787 None:intergenic
TGTCTTGCACGCTTCAAAGA+AGG 0.457699 4.4:-77086544 None:intergenic
ATACCCACAAGATCACTAAC+AGG 0.465851 4.4:-77087225 None:intergenic
AACAGTGCTCCGTCGACGCC+GGG 0.468408 4.4:+77086163 MS.gene50266:CDS
ATTATGAAGTTGCCGAGGAA+TGG 0.468878 4.4:+77087285 MS.gene50266:CDS
TGTTTATGGATTGAATACTA+AGG 0.472188 4.4:+77086816 MS.gene50266:CDS
ATGCATTTGGCTGCTCAAGC+TGG 0.474694 4.4:+77086667 MS.gene50266:CDS
ACAAGATCACTAACAGGAAC+AGG 0.475033 4.4:-77087219 None:intergenic
CGTGAAGGGTCCGCCGGAAG+AGG 0.475778 4.4:-77086319 None:intergenic
TCCGACGAAACCAGCAGCTC+CGG 0.477531 4.4:-77086446 None:intergenic
GCGCTTAAACGCCGCGGAGA+CGG 0.482259 4.4:+77086484 MS.gene50266:CDS
GTTCCTGTTAGTGATCTTGT+GGG 0.482574 4.4:+77087222 MS.gene50266:CDS
CGGAGAATCCGTTCCGTGAC+CGG 0.489325 4.4:-77086414 None:intergenic
TCCGTTCCTCCGCTAGAGTC+CGG 0.497044 4.4:+77086395 MS.gene50266:CDS
CTCCAAAGACAAAGCTTCGC+CGG 0.498173 4.4:-77086241 None:intergenic
AGTTATGCGCAGAGAGAGCT+TGG 0.500038 4.4:+77087336 MS.gene50266:CDS
CGGACACGTTTCTCCCAAAC+GGG 0.500537 4.4:-77086376 None:intergenic
GATACTGCTGAGAAGAGTAC+TGG 0.501421 4.4:+77087135 MS.gene50266:CDS
CTTCTTACTTCAACCGTGTA+CGG 0.502910 4.4:+77086206 MS.gene50266:CDS
ACGGGACCACCCCAGTTTGA+AGG 0.504384 4.4:-77086358 None:intergenic
GAGAGAGCTTGGTTATAAAC+CGG 0.506070 4.4:+77087347 MS.gene50266:CDS
GGATTCTCCGTTCTTGTGAC+CGG 0.510300 4.4:+77086427 MS.gene50266:CDS
GACCGCCGTGAAGGGTCCGC+CGG 0.515234 4.4:-77086325 None:intergenic
TGTTCCTGTTAGTGATCTTG+TGG 0.519953 4.4:+77087221 MS.gene50266:CDS
ACGGACACGTTTCTCCCAAA+CGG 0.521018 4.4:-77086377 None:intergenic
AGTATGCGCGATTTCTTCAC+CGG 0.527758 4.4:-77086902 None:intergenic
CTCCTCAATGACCGCCGTGA+AGG 0.532413 4.4:-77086334 None:intergenic
GCCCAAACAATTGAAGGTTG+AGG 0.535698 4.4:-77086781 None:intergenic
ACCGGAGCTGCTGGTTTCGT+CGG 0.536422 4.4:+77086445 MS.gene50266:CDS
TGGAAGTGGTGGGAAGAAGA+GGG 0.543352 4.4:+77087155 MS.gene50266:CDS
TTCACTGTTTATGGTCCTTG+GGG 0.546844 4.4:+77086967 MS.gene50266:CDS
TTGAAAACCCTCAACTGTGC+CGG 0.546878 4.4:-77087180 None:intergenic
ACCCTTCACGGCGGTCATTG+AGG 0.548511 4.4:+77086332 MS.gene50266:CDS
ATTGAGGAGTCCTTCAAACT+GGG 0.549385 4.4:+77086348 MS.gene50266:CDS
TTATATTGATGATATTGTGA+GGG 0.550359 4.4:+77087095 MS.gene50266:CDS
GAGGAGAAGTCGCCGGAGAA+CGG 0.554120 4.4:-77086300 None:intergenic
AACTGAACATCCCCATTCCT+CGG 0.554769 4.4:-77087297 None:intergenic
TCCTCAATGACCGCCGTGAA+GGG 0.559853 4.4:-77086333 None:intergenic
TAACAGTGCTCCGTCGACGC+CGG 0.562127 4.4:+77086162 MS.gene50266:CDS
GTAGCGGCATACAAACTAGC+AGG 0.564565 4.4:-77086871 None:intergenic
TTGAACTTTCCCGGCGTCGA+CGG 0.565515 4.4:-77086172 None:intergenic
CGAATCATGGTACGGTTGCG+AGG 0.566838 4.4:+77087064 MS.gene50266:CDS
TTCACCGGCTTTCTTAGTAG+CGG 0.567698 4.4:-77086887 None:intergenic
GAGAAGCGTGCCACCGTACA+CGG 0.567964 4.4:-77086219 None:intergenic
AGCAGCTCCGGTCACAAGAA+CGG 0.569637 4.4:-77086434 None:intergenic
ACCGGACTCTAGCGGAGGAA+CGG 0.574251 4.4:-77086396 None:intergenic
CTTATATTGATGATATTGTG+AGG 0.578974 4.4:+77087094 MS.gene50266:CDS
GAAGTGGTGGGAAGAAGAGG+GGG 0.582983 4.4:+77087157 MS.gene50266:CDS
TGAGGCGGCGAATCATGGTA+CGG 0.585506 4.4:+77087056 MS.gene50266:CDS
CCGGTGACGGATCTTCAGGC+TGG 0.586013 4.4:+77087366 MS.gene50266:CDS
AGCTGGTGTGAGGTATGCTA+TGG 0.586552 4.4:+77086684 MS.gene50266:CDS
AGGTATGCTATGGAGAATCC+TGG 0.586560 4.4:+77086694 MS.gene50266:CDS
CCAGCCTGAAGATCCGTCAC+CGG 0.600499 4.4:-77087366 None:intergenic
GGCGACTTCTCCTCTTCCGG+CGG 0.605382 4.4:+77086309 MS.gene50266:CDS
GGAAGTGGTGGGAAGAAGAG+GGG 0.607007 4.4:+77087156 MS.gene50266:CDS
CCTCCGCTAGAGTCCGGTCA+CGG 0.608900 4.4:+77086401 MS.gene50266:CDS
TATGAAGTTGCCGAGGAATG+GGG 0.609964 4.4:+77087287 MS.gene50266:CDS
GCTAGAGTCCGGTCACGGAA+CGG 0.610673 4.4:+77086406 MS.gene50266:CDS
GAATCATGGTACGGTTGCGA+GGG 0.611200 4.4:+77087065 MS.gene50266:CDS
GCCGGAAGAGGAGAAGTCGC+CGG 0.613076 4.4:-77086307 None:intergenic
TATGCCGCTACTAAGAAAGC+CGG 0.617216 4.4:+77086883 MS.gene50266:CDS
GCTTGGTTATAAACCGGTGA+CGG 0.619019 4.4:+77087353 MS.gene50266:CDS
GAGAAGAGTACTGGAAGTGG+TGG 0.623154 4.4:+77087144 MS.gene50266:CDS
GTTTGAAGAAATTTGTGAGG+TGG 0.624134 4.4:+77087388 MS.gene50266:CDS
TGGCTGCTCAAGCTGGTGTG+AGG 0.625330 4.4:+77086674 MS.gene50266:CDS
GCCATATCAGGCCTACCCCA+AGG 0.630669 4.4:-77086982 None:intergenic
GCTGAGAAGAGTACTGGAAG+TGG 0.633054 4.4:+77087141 MS.gene50266:CDS
GACGCCGGGAAAGTTCAAGA+TGG 0.634115 4.4:+77086177 MS.gene50266:CDS
TCCGCGGCGCTTAAACGCCG+CGG 0.635349 4.4:+77086478 MS.gene50266:CDS
TCCGCGGCGTTTAAGCGCCG+CGG 0.637292 4.4:-77086479 None:intergenic
CTGGTTTGAAGAAATTTGTG+AGG 0.647230 4.4:+77087385 MS.gene50266:CDS
AGGAGTCCTTCAAACTGGGG+TGG 0.647390 4.4:+77086352 MS.gene50266:CDS
TCCCTAAAACACCGTCTCCG+CGG 0.649971 4.4:-77086495 None:intergenic
AGAAGAGTACTGGAAGTGGT+GGG 0.649979 4.4:+77087145 MS.gene50266:CDS
GTTCCGTGACCGGACTCTAG+CGG 0.650330 4.4:-77086404 None:intergenic
GGAATATTATGAAGTTGCCG+AGG 0.656687 4.4:+77087280 MS.gene50266:CDS
CTTACTTCAACCGTGTACGG+TGG 0.659507 4.4:+77086209 MS.gene50266:CDS
CCGTGACCGGACTCTAGCGG+AGG 0.659987 4.4:-77086401 None:intergenic
TATATTGATGATATTGTGAG+GGG 0.666488 4.4:+77087096 MS.gene50266:CDS
ATCACTAACAGGAACAGGCG+AGG 0.666779 4.4:-77087214 None:intergenic
TGTTGAAGACTAAGGCGAAG+AGG 0.671437 4.4:+77087259 MS.gene50266:CDS
TTACTATGAACATAAGAACC+AGG 0.683714 4.4:-77086712 None:intergenic
TTGAGGAGTCCTTCAAACTG+GGG 0.690015 4.4:+77086349 MS.gene50266:CDS
CGTCGGAACACATGTCTCCG+CGG 0.690793 4.4:+77086462 MS.gene50266:CDS
TTCCGGCGGACCCTTCACGG+CGG 0.735183 4.4:+77086323 MS.gene50266:CDS
ATATTGATGATATTGTGAGG+GGG 0.741076 4.4:+77087097 MS.gene50266:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATACTTATAATCATATCTA+TGG + chr4.4:77086919-77086938 MS.gene50266:CDS 20.0%
!!! TTATATTGATGATATTGTGA+GGG + chr4.4:77087095-77087114 MS.gene50266:CDS 20.0%
! AAAAAGAAATAAGCCATATC+AGG - chr4.4:77086997-77087016 None:intergenic 25.0%
! ATCTGAATTACTATTCTTCT+GGG + chr4.4:77087412-77087431 MS.gene50266:CDS 25.0%
! TATCTGAATTACTATTCTTC+TGG + chr4.4:77087411-77087430 MS.gene50266:CDS 25.0%
!! CTTATATTGATGATATTGTG+AGG + chr4.4:77087094-77087113 MS.gene50266:CDS 25.0%
!! TATATTGATGATATTGTGAG+GGG + chr4.4:77087096-77087115 MS.gene50266:CDS 25.0%
!! TGTTTATGGATTGAATACTA+AGG + chr4.4:77086816-77086835 MS.gene50266:CDS 25.0%
!!! TTCACTAGAGATATTTTGAA+GGG + chr4.4:77087015-77087034 MS.gene50266:CDS 25.0%
!!! TTTCACTAGAGATATTTTGA+AGG + chr4.4:77087014-77087033 MS.gene50266:CDS 25.0%
AAATGCATCACATGAGTAAA+AGG - chr4.4:77086655-77086674 None:intergenic 30.0%
ATCTATGGTCTTTCTTTAAC+TGG + chr4.4:77086934-77086953 MS.gene50266:CDS 30.0%
TATGTTCATAGTAACATTGC+TGG + chr4.4:77086721-77086740 MS.gene50266:CDS 30.0%
TTACTATGAACATAAGAACC+AGG - chr4.4:77086715-77086734 None:intergenic 30.0%
! ATATTGATGATATTGTGAGG+GGG + chr4.4:77087097-77087116 MS.gene50266:CDS 30.0%
! TTGAGGTTTTTCACTGTTTA+TGG + chr4.4:77086958-77086977 MS.gene50266:CDS 30.0%
! TTTGTTAAGGAAGCTTTTTG+AGG + chr4.4:77086624-77086643 MS.gene50266:CDS 30.0%
! TTTTACTCATGTGATGCATT+TGG + chr4.4:77086654-77086673 MS.gene50266:CDS 30.0%
!! TAAGGTTCCTTTTTCTGAAA+GGG + chr4.4:77086834-77086853 MS.gene50266:CDS 30.0%
!! TCACTAGAGATATTTTGAAG+GGG + chr4.4:77087016-77087035 MS.gene50266:CDS 30.0%
!!! GAAGACTATTCCTATTTTTG+AGG + chr4.4:77087038-77087057 MS.gene50266:CDS 30.0%
!!! TGGTTTTGTTAATTTGCTTG+AGG + chr4.4:77086741-77086760 MS.gene50266:CDS 30.0%
ATCCTCAACCTTCAATTGTT+TGG + chr4.4:77086779-77086798 MS.gene50266:CDS 35.0%
GTTCTATCCCTTTCAGAAAA+AGG - chr4.4:77086844-77086863 None:intergenic 35.0%
GTTTGAAGAAATTTGTGAGG+TGG + chr4.4:77087388-77087407 MS.gene50266:CDS 35.0%
TCCTCAACCTTCAATTGTTT+GGG + chr4.4:77086780-77086799 MS.gene50266:CDS 35.0%
TTTCACTGTTTATGGTCCTT+GGG + chr4.4:77086966-77086985 MS.gene50266:CDS 35.0%
! ACAGTTGAGGGTTTTCAATT+TGG + chr4.4:77087185-77087204 MS.gene50266:CDS 35.0%
! ACTGGTGTTTTCATTGTTGA+AGG + chr4.4:77086583-77086602 MS.gene50266:CDS 35.0%
! AGTTGAGGGTTTTCAATTTG+GGG + chr4.4:77087187-77087206 MS.gene50266:CDS 35.0%
! CAGTTGAGGGTTTTCAATTT+GGG + chr4.4:77087186-77087205 MS.gene50266:CDS 35.0%
! CTAAGGTTCCTTTTTCTGAA+AGG + chr4.4:77086833-77086852 MS.gene50266:CDS 35.0%
! CTGGTTTGAAGAAATTTGTG+AGG + chr4.4:77087385-77087404 MS.gene50266:CDS 35.0%
! TAGTGATCTTGTGGGTATTT+TGG + chr4.4:77087230-77087249 MS.gene50266:CDS 35.0%
! TTTTCACTGTTTATGGTCCT+TGG + chr4.4:77086965-77086984 MS.gene50266:CDS 35.0%
!! GCTAGTTCTAGTTCTGTTTA+TGG + chr4.4:77086802-77086821 MS.gene50266:CDS 35.0%
!! GTCTTTCTTTAACTGGTTTG+AGG + chr4.4:77086941-77086960 MS.gene50266:CDS 35.0%
!! TCAATGATGCTGCTTTGTTA+AGG + chr4.4:77086611-77086630 MS.gene50266:CDS 35.0%
!!! GACTATTCCTATTTTTGAGG+CGG + chr4.4:77087041-77087060 MS.gene50266:CDS 35.0%
ACAAGATCACTAACAGGAAC+AGG - chr4.4:77087222-77087241 None:intergenic 40.0%
ATACCCACAAGATCACTAAC+AGG - chr4.4:77087228-77087247 None:intergenic 40.0%
ATTATGAAGTTGCCGAGGAA+TGG + chr4.4:77087285-77087304 MS.gene50266:CDS 40.0%
ATTGAGGAGTCCTTCAAACT+GGG + chr4.4:77086348-77086367 MS.gene50266:CDS 40.0%
CTTCTTACTTCAACCGTGTA+CGG + chr4.4:77086206-77086225 MS.gene50266:CDS 40.0%
GAACTAGCCCAAACAATTGA+AGG - chr4.4:77086790-77086809 None:intergenic 40.0%
GAGAGAGCTTGGTTATAAAC+CGG + chr4.4:77087347-77087366 MS.gene50266:CDS 40.0%
GGAATATTATGAAGTTGCCG+AGG + chr4.4:77087280-77087299 MS.gene50266:CDS 40.0%
GTTCCTGTTAGTGATCTTGT+GGG + chr4.4:77087222-77087241 MS.gene50266:CDS 40.0%
TGTTCCTGTTAGTGATCTTG+TGG + chr4.4:77087221-77087240 MS.gene50266:CDS 40.0%
TTATGAAGTTGCCGAGGAAT+GGG + chr4.4:77087286-77087305 MS.gene50266:CDS 40.0%
TTCACTGTTTATGGTCCTTG+GGG + chr4.4:77086967-77086986 MS.gene50266:CDS 40.0%
TTCTCCATCTTGAACTTTCC+CGG - chr4.4:77086184-77086203 None:intergenic 40.0%
! GATCTTCTTTTTCCGTTCTC+CGG + chr4.4:77086288-77086307 MS.gene50266:CDS 40.0%
!! ATCTTGTGGGTATTTTGGAG+AGG + chr4.4:77087235-77087254 MS.gene50266:CDS 40.0%
AACTGAACATCCCCATTCCT+CGG - chr4.4:77087300-77087319 None:intergenic 45.0%
AGGTATGCTATGGAGAATCC+TGG + chr4.4:77086694-77086713 MS.gene50266:CDS 45.0%
AGTATGCGCGATTTCTTCAC+CGG - chr4.4:77086905-77086924 None:intergenic 45.0%
CATTGAGGAGTCCTTCAAAC+TGG + chr4.4:77086347-77086366 MS.gene50266:CDS 45.0%
GCCCAAACAATTGAAGGTTG+AGG - chr4.4:77086784-77086803 None:intergenic 45.0%
GCTTGGTTATAAACCGGTGA+CGG + chr4.4:77087353-77087372 MS.gene50266:CDS 45.0%
TATGAAGTTGCCGAGGAATG+GGG + chr4.4:77087287-77087306 MS.gene50266:CDS 45.0%
TATGCCGCTACTAAGAAAGC+CGG + chr4.4:77086883-77086902 MS.gene50266:CDS 45.0%
TGATTCGCCGCCTCAAAAAT+AGG - chr4.4:77087051-77087070 None:intergenic 45.0%
TGTCTTGCACGCTTCAAAGA+AGG - chr4.4:77086547-77086566 None:intergenic 45.0%
TGTTGAAGACTAAGGCGAAG+AGG + chr4.4:77087259-77087278 MS.gene50266:CDS 45.0%
TTGAAAACCCTCAACTGTGC+CGG - chr4.4:77087183-77087202 None:intergenic 45.0%
TTGAGGAGTCCTTCAAACTG+GGG + chr4.4:77086349-77086368 MS.gene50266:CDS 45.0%
! GATACTGCTGAGAAGAGTAC+TGG + chr4.4:77087135-77087154 MS.gene50266:CDS 45.0%
!! AGAAGAGTACTGGAAGTGGT+GGG + chr4.4:77087145-77087164 MS.gene50266:CDS 45.0%
!! GATATTGTGAGGGGGTGTTT+AGG + chr4.4:77087105-77087124 MS.gene50266:CDS 45.0%
!! TTCACCGGCTTTCTTAGTAG+CGG - chr4.4:77086890-77086909 None:intergenic 45.0%
!!! ATTTTTGAGGCGGCGAATCA+TGG + chr4.4:77087051-77087070 MS.gene50266:CDS 45.0%
!!! CAAGCACTTTTGGAACGTAC+TGG + chr4.4:77086565-77086584 MS.gene50266:CDS 45.0%
ACGGACACGTTTCTCCCAAA+CGG - chr4.4:77086380-77086399 None:intergenic 50.0%
AGCTGGTGTGAGGTATGCTA+TGG + chr4.4:77086684-77086703 MS.gene50266:CDS 50.0%
AGTTATGCGCAGAGAGAGCT+TGG + chr4.4:77087336-77087355 MS.gene50266:CDS 50.0%
ATCACTAACAGGAACAGGCG+AGG - chr4.4:77087217-77087236 None:intergenic 50.0%
ATGCATTTGGCTGCTCAAGC+TGG + chr4.4:77086667-77086686 MS.gene50266:CDS 50.0%
CTCCAAAGACAAAGCTTCGC+CGG - chr4.4:77086244-77086263 None:intergenic 50.0%
CTGTTTATGGTCCTTGGGGT+AGG + chr4.4:77086971-77086990 MS.gene50266:CDS 50.0%
CTTACTTCAACCGTGTACGG+TGG + chr4.4:77086209-77086228 MS.gene50266:CDS 50.0%
GAATCATGGTACGGTTGCGA+GGG + chr4.4:77087065-77087084 MS.gene50266:CDS 50.0%
GGAGAGGCTGTTGAAGACTA+AGG + chr4.4:77087251-77087270 MS.gene50266:CDS 50.0%
GGATTCTCCGTTCTTGTGAC+CGG + chr4.4:77086427-77086446 MS.gene50266:CDS 50.0%
GTAGCGGCATACAAACTAGC+AGG - chr4.4:77086874-77086893 None:intergenic 50.0%
! GCGTGCAAGACAAGCACTTT+TGG + chr4.4:77086555-77086574 MS.gene50266:CDS 50.0%
! TGGAAGTGGTGGGAAGAAGA+GGG + chr4.4:77087155-77087174 MS.gene50266:CDS 50.0%
!! GAGAAGAGTACTGGAAGTGG+TGG + chr4.4:77087144-77087163 MS.gene50266:CDS 50.0%
!! GCTGAGAAGAGTACTGGAAG+TGG + chr4.4:77087141-77087160 MS.gene50266:CDS 50.0%
!! TAAACCGGTGACGGATCTTC+AGG + chr4.4:77087362-77087381 MS.gene50266:CDS 50.0%
AAACTGGGGTGGTCCCGTTT+GGG + chr4.4:77086363-77086382 MS.gene50266:CDS 55.0%
AGCAGCTCCGGTCACAAGAA+CGG - chr4.4:77086437-77086456 None:intergenic 55.0%
AGGAGTCCTTCAAACTGGGG+TGG + chr4.4:77086352-77086371 MS.gene50266:CDS 55.0%
CGAATCATGGTACGGTTGCG+AGG + chr4.4:77087064-77087083 MS.gene50266:CDS 55.0%
CGGACACGTTTCTCCCAAAC+GGG - chr4.4:77086379-77086398 None:intergenic 55.0%
GACGCCGGGAAAGTTCAAGA+TGG + chr4.4:77086177-77086196 MS.gene50266:CDS 55.0%
TCCCTAAAACACCGTCTCCG+CGG - chr4.4:77086498-77086517 None:intergenic 55.0%
TCCTCAATGACCGCCGTGAA+GGG - chr4.4:77086336-77086355 None:intergenic 55.0%
TCCTTGGGGTAGGCCTGATA+TGG + chr4.4:77086981-77087000 MS.gene50266:CDS 55.0%
TGAGGCGGCGAATCATGGTA+CGG + chr4.4:77087056-77087075 MS.gene50266:CDS 55.0%
! CTCCGGCGAAGCTTTGTCTT+TGG + chr4.4:77086239-77086258 MS.gene50266:CDS 55.0%
! CTGGAAGTGGTGGGAAGAAG+AGG + chr4.4:77087154-77087173 MS.gene50266:CDS 55.0%
! GAAGTGGTGGGAAGAAGAGG+GGG + chr4.4:77087157-77087176 MS.gene50266:CDS 55.0%
! GGAAGTGGTGGGAAGAAGAG+GGG + chr4.4:77087156-77087175 MS.gene50266:CDS 55.0%
! TTGAACTTTCCCGGCGTCGA+CGG - chr4.4:77086175-77086194 None:intergenic 55.0%
!! GAGGGGGTGTTTAGGTGCTT+TGG + chr4.4:77087113-77087132 MS.gene50266:CDS 55.0%
ACCCTTCACGGCGGTCATTG+AGG + chr4.4:77086332-77086351 MS.gene50266:CDS 60.0%
ACCGGACTCTAGCGGAGGAA+CGG - chr4.4:77086399-77086418 None:intergenic 60.0%
ACGGGACCACCCCAGTTTGA+AGG - chr4.4:77086361-77086380 None:intergenic 60.0%
CAAACTGGGGTGGTCCCGTT+TGG + chr4.4:77086362-77086381 MS.gene50266:CDS 60.0%
CCAGCCTGAAGATCCGTCAC+CGG - chr4.4:77087369-77087388 None:intergenic 60.0%
CGGAGAATCCGTTCCGTGAC+CGG - chr4.4:77086417-77086436 None:intergenic 60.0%
CGTCGGAACACATGTCTCCG+CGG + chr4.4:77086462-77086481 MS.gene50266:CDS 60.0%
CTCCTCAATGACCGCCGTGA+AGG - chr4.4:77086337-77086356 None:intergenic 60.0%
GAGGAGAAGTCGCCGGAGAA+CGG - chr4.4:77086303-77086322 None:intergenic 60.0%
GCCATATCAGGCCTACCCCA+AGG - chr4.4:77086985-77087004 None:intergenic 60.0%
GCTAGAGTCCGGTCACGGAA+CGG + chr4.4:77086406-77086425 MS.gene50266:CDS 60.0%
GTTCCGTGACCGGACTCTAG+CGG - chr4.4:77086407-77086426 None:intergenic 60.0%
GTTCTTGTGACCGGAGCTGC+TGG + chr4.4:77086436-77086455 MS.gene50266:CDS 60.0%
TAACAGTGCTCCGTCGACGC+CGG + chr4.4:77086162-77086181 MS.gene50266:CDS 60.0%
TCCGACGAAACCAGCAGCTC+CGG - chr4.4:77086449-77086468 None:intergenic 60.0%
TCCGGCGACTTCTCCTCTTC+CGG + chr4.4:77086306-77086325 MS.gene50266:CDS 60.0%
TCCGTTCCTCCGCTAGAGTC+CGG + chr4.4:77086395-77086414 MS.gene50266:CDS 60.0%
TGGCTGCTCAAGCTGGTGTG+AGG + chr4.4:77086674-77086693 MS.gene50266:CDS 60.0%
TGTACGGTGGCACGCTTCTC+CGG + chr4.4:77086222-77086241 MS.gene50266:CDS 60.0%
! GAGAAGCGTGCCACCGTACA+CGG - chr4.4:77086222-77086241 None:intergenic 60.0%
! GCCGCGGAGACGGTGTTTTA+GGG + chr4.4:77086494-77086513 MS.gene50266:CDS 60.0%
!! ACCGGAGCTGCTGGTTTCGT+CGG + chr4.4:77086445-77086464 MS.gene50266:CDS 60.0%
AACAGTGCTCCGTCGACGCC+GGG + chr4.4:77086163-77086182 MS.gene50266:CDS 65.0%
CCTCCGCTAGAGTCCGGTCA+CGG + chr4.4:77086401-77086420 MS.gene50266:CDS 65.0%
CTCTTCCGGCGGACCCTTCA+CGG + chr4.4:77086320-77086339 MS.gene50266:CDS 65.0%
GCCGGAAGAGGAGAAGTCGC+CGG - chr4.4:77086310-77086329 None:intergenic 65.0%
GCGCTTAAACGCCGCGGAGA+CGG + chr4.4:77086484-77086503 MS.gene50266:CDS 65.0%
GGCGACTTCTCCTCTTCCGG+CGG + chr4.4:77086309-77086328 MS.gene50266:CDS 65.0%
TGGTGGGAAGAAGAGGGGGC+CGG + chr4.4:77087161-77087180 MS.gene50266:CDS 65.0%
! CGCCGCGGAGACGGTGTTTT+AGG + chr4.4:77086493-77086512 MS.gene50266:CDS 65.0%
!! CCGGTGACGGATCTTCAGGC+TGG + chr4.4:77087366-77087385 MS.gene50266:CDS 65.0%
AGAGGGGGCCGGCACAGTTG+AGG + chr4.4:77087172-77087191 MS.gene50266:CDS 70.0%
CCGTGACCGGACTCTAGCGG+AGG - chr4.4:77086404-77086423 None:intergenic 70.0%
CGTGAAGGGTCCGCCGGAAG+AGG - chr4.4:77086322-77086341 None:intergenic 70.0%
GAGGGGGCCGGCACAGTTGA+GGG + chr4.4:77087173-77087192 MS.gene50266:CDS 70.0%
TCCGCGGCGCTTAAACGCCG+CGG + chr4.4:77086478-77086497 MS.gene50266:CDS 70.0%
TTCCGGCGGACCCTTCACGG+CGG + chr4.4:77086323-77086342 MS.gene50266:CDS 70.0%
! TCCGCGGCGTTTAAGCGCCG+CGG - chr4.4:77086482-77086501 None:intergenic 70.0%
GACCGCCGTGAAGGGTCCGC+CGG - chr4.4:77086328-77086347 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 77086130 77087452 77086130 ID=MS.gene50266
chr4.4 mRNA 77086130 77087452 77086130 ID=MS.gene50266.t1;Parent=MS.gene50266
chr4.4 exon 77086130 77087452 77086130 ID=MS.gene50266.t1.exon1;Parent=MS.gene50266.t1
chr4.4 CDS 77086130 77087452 77086130 ID=cds.MS.gene50266.t1;Parent=MS.gene50266.t1
Gene Sequence

>MS.gene50266

ATGTCTCAGCTGAAGCAAATGTCGCACGCTGATAACAGTGCTCCGTCGACGCCGGGAAAGTTCAAGATGGAGAAAGCTTCTTACTTCAACCGTGTACGGTGGCACGCTTCTCCGGCGAAGCTTTGTCTTTGGAGTTTTGTTTTCTCAGCTGCGATCTTGATCTTCTTTTTCCGTTCTCCGGCGACTTCTCCTCTTCCGGCGGACCCTTCACGGCGGTCATTGAGGAGTCCTTCAAACTGGGGTGGTCCCGTTTGGGAGAAACGTGTCCGTTCCTCCGCTAGAGTCCGGTCACGGAACGGATTCTCCGTTCTTGTGACCGGAGCTGCTGGTTTCGTCGGAACACATGTCTCCGCGGCGCTTAAACGCCGCGGAGACGGTGTTTTAGGGATTGATAATTTCAATGACTATTATGATCCTTCTTTGAAGCGTGCAAGACAAGCACTTTTGGAACGTACTGGTGTTTTCATTGTTGAAGGTGATATCAATGATGCTGCTTTGTTAAGGAAGCTTTTTGAGGTTGTTCCTTTTACTCATGTGATGCATTTGGCTGCTCAAGCTGGTGTGAGGTATGCTATGGAGAATCCTGGTTCTTATGTTCATAGTAACATTGCTGGTTTTGTTAATTTGCTTGAGGTTTGTAAAAGTGTTAATCCTCAACCTTCAATTGTTTGGGCTAGTTCTAGTTCTGTTTATGGATTGAATACTAAGGTTCCTTTTTCTGAAAGGGATAGAACTGATCAACCTGCTAGTTTGTATGCCGCTACTAAGAAAGCCGGTGAAGAAATCGCGCATACTTATAATCATATCTATGGTCTTTCTTTAACTGGTTTGAGGTTTTTCACTGTTTATGGTCCTTGGGGTAGGCCTGATATGGCTTATTTCTTTTTCACTAGAGATATTTTGAAGGGGAAGACTATTCCTATTTTTGAGGCGGCGAATCATGGTACGGTTGCGAGGGATTTTACTTATATTGATGATATTGTGAGGGGGTGTTTAGGTGCTTTGGATACTGCTGAGAAGAGTACTGGAAGTGGTGGGAAGAAGAGGGGGCCGGCACAGTTGAGGGTTTTCAATTTGGGGAATACCTCGCCTGTTCCTGTTAGTGATCTTGTGGGTATTTTGGAGAGGCTGTTGAAGACTAAGGCGAAGAGGAATATTATGAAGTTGCCGAGGAATGGGGATGTTCAGTTTACTCATGCGAATATTAGTTATGCGCAGAGAGAGCTTGGTTATAAACCGGTGACGGATCTTCAGGCTGGTTTGAAGAAATTTGTGAGGTGGTATCTGAATTACTATTCTTCTGGGAAGAAAGCTGTTGAGTGA
Protein sequence

>MS.gene50266.t1

MSQLKQMSHADNSAPSTPGKFKMEKASYFNRVRWHASPAKLCLWSFVFSAAILIFFFRSPATSPLPADPSRRSLRSPSNWGGPVWEKRVRSSARVRSRNGFSVLVTGAAGFVGTHVSAALKRRGDGVLGIDNFNDYYDPSLKRARQALLERTGVFIVEGDINDAALLRKLFEVVPFTHVMHLAAQAGVRYAMENPGSYVHSNIAGFVNLLEVCKSVNPQPSIVWASSSSVYGLNTKVPFSERDRTDQPASLYAATKKAGEEIAHTYNHIYGLSLTGLRFFTVYGPWGRPDMAYFFFTRDILKGKTIPIFEAANHGTVARDFTYIDDIVRGCLGALDTAEKSTGSGGKKRGPAQLRVFNLGNTSPVPVSDLVGILERLLKTKAKRNIMKLPRNGDVQFTHANISYAQRELGYKPVTDLQAGLKKFVRWYLNYYSSGKKAVE