AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066580.t1 MTR_4g024600 99.773 440 1 0 1 440 1 440 0.0 907
MS.gene066580.t1 MTR_0354s0040 82.629 426 66 3 15 440 4 421 0.0 728
MS.gene066580.t1 MTR_4g102270 69.431 422 125 3 11 432 7 424 0.0 603
MS.gene066580.t1 MTR_4g129820 67.431 436 132 4 8 440 6 434 0.0 593
MS.gene066580.t1 MTR_3g084090 66.047 430 134 3 13 432 3 430 0.0 585
MS.gene066580.t1 MTR_5g011940 76.294 367 85 1 69 433 56 422 0.0 581
MS.gene066580.t1 MTR_0034s0060 27.545 334 213 8 102 428 138 449 9.11e-22 98.6
MS.gene066580.t1 MTR_8g096880 28.614 332 199 12 103 412 8 323 1.45e-21 95.9
MS.gene066580.t1 MTR_8g096880 29.042 334 195 13 103 412 8 323 2.08e-21 94.7
MS.gene066580.t1 MTR_2g073240 26.822 343 208 8 102 430 20 333 2.62e-20 94.0
MS.gene066580.t1 MTR_2g032100 26.087 345 219 12 103 427 73 401 2.65e-20 92.8
MS.gene066580.t1 MTR_5g009170 26.039 361 230 12 91 433 38 379 3.38e-20 92.4
MS.gene066580.t1 MTR_8g099535 27.576 330 205 12 102 412 5 319 7.92e-20 90.9
MS.gene066580.t1 MTR_1g009600 25.519 337 220 10 102 430 6 319 3.17e-19 90.9
MS.gene066580.t1 MTR_4g120030 25.397 441 251 17 8 425 20 405 6.54e-19 89.0
MS.gene066580.t1 MTR_5g009160 26.923 338 203 11 99 412 2 319 1.07e-18 87.4
MS.gene066580.t1 MTR_1g088480 25.816 337 202 12 98 425 115 412 1.20e-18 88.2
MS.gene066580.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene066580.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene066580.t1 MTR_4g063600 24.471 331 212 11 98 421 29 328 2.91e-17 83.2
MS.gene066580.t1 MTR_8g096880 28.041 296 176 11 103 376 8 288 8.39e-17 81.3
MS.gene066580.t1 MTR_4g063600 24.773 331 208 12 98 421 29 325 2.90e-16 80.1
MS.gene066580.t1 MTR_7g115080 25.000 336 210 13 103 427 31 335 2.48e-15 77.8
MS.gene066580.t1 MTR_7g115080 25.000 336 210 13 103 427 65 369 2.64e-15 77.8
MS.gene066580.t1 MTR_7g115080 25.000 336 210 13 103 427 44 348 2.70e-15 77.8
MS.gene066580.t1 MTR_1g080950 23.810 399 247 16 41 427 18 371 4.46e-15 77.0
MS.gene066580.t1 MTR_2g032100 29.630 189 127 5 103 288 73 258 6.25e-15 75.5
MS.gene066580.t1 MTR_1g080950 24.189 339 214 12 101 427 28 335 8.07e-15 76.3
MS.gene066580.t1 MTR_2g096660 24.324 333 210 12 98 421 35 334 1.67e-14 75.1
MS.gene066580.t1 MTR_4g101940 86.667 30 4 0 173 202 42 71 2.71e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066580.t1 AT4G00110 86.667 435 51 4 4 438 1 428 0.0 770
MS.gene066580.t1 AT1G02000 84.828 435 59 5 7 439 1 430 0.0 751
MS.gene066580.t1 AT2G45310 78.851 435 88 3 7 438 1 434 0.0 693
MS.gene066580.t1 AT4G12250 73.059 438 105 4 7 437 1 432 0.0 621
MS.gene066580.t1 AT3G23820 77.557 352 79 0 81 432 92 443 0.0 582
MS.gene066580.t1 AT4G30440 67.907 430 126 4 11 438 6 425 0.0 564
MS.gene066580.t1 AT4G23920 27.350 351 215 12 102 430 4 336 4.19e-23 100
MS.gene066580.t1 AT1G12780 28.657 335 197 13 102 412 8 324 1.10e-22 99.0
MS.gene066580.t1 AT1G63180 28.443 334 199 12 102 412 8 324 5.08e-21 94.4
MS.gene066580.t1 AT1G78570 26.647 334 216 8 102 428 8 319 8.81e-21 95.5
MS.gene066580.t1 AT3G14790 26.647 334 216 8 102 428 11 322 2.27e-20 94.4
MS.gene066580.t1 AT3G14790 26.647 334 216 8 102 428 8 319 2.54e-20 94.4
MS.gene066580.t1 AT4G10960 26.891 357 209 13 102 430 5 337 7.75e-20 90.9
MS.gene066580.t1 AT1G30620 25.507 345 221 11 103 427 72 400 4.10e-19 89.4
MS.gene066580.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene066580.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene066580.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene066580.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene066580.t1 AT1G53500 26.393 341 208 8 102 428 10 321 9.08e-19 89.4
MS.gene066580.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene066580.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene066580.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene066580.t1 AT5G44480 24.709 344 225 10 103 427 97 425 2.18e-18 87.4
MS.gene066580.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene066580.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene066580.t1 AT3G53520 25.294 340 215 10 98 430 117 424 1.40e-17 85.1
MS.gene066580.t1 AT1G64440 25.281 356 220 12 102 434 4 336 1.42e-17 84.0
MS.gene066580.t1 AT2G47650 24.122 427 252 16 32 426 35 421 3.98e-17 83.6
MS.gene066580.t1 AT4G20460 25.212 353 227 12 96 427 64 400 4.53e-17 83.2
MS.gene066580.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene066580.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene066580.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene066580.t1 AT2G28760 25.225 333 207 13 98 421 59 358 8.34e-17 82.0
MS.gene066580.t1 AT1G63180 28.188 298 175 11 102 376 8 289 9.29e-17 81.3
MS.gene066580.t1 AT3G53520 25.000 340 214 11 98 430 117 422 1.72e-15 78.6
MS.gene066580.t1 AT2G47650 24.249 433 250 18 32 426 35 427 3.98e-15 77.4
MS.gene066580.t1 AT5G59290 24.085 328 207 12 103 421 47 341 7.57e-15 76.3
MS.gene066580.t1 AT5G59290 24.085 328 207 12 103 421 32 326 8.39e-15 75.9
MS.gene066580.t1 AT5G59290 24.085 328 207 12 103 421 3 297 9.21e-15 75.5
MS.gene066580.t1 AT5G59290 24.085 328 207 12 103 421 25 319 1.03e-14 75.5
MS.gene066580.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene066580.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene066580.t1 AT3G53520 27.083 240 153 6 98 331 117 340 2.60e-14 74.3
MS.gene066580.t1 AT3G53520 24.011 354 225 12 98 430 117 447 3.55e-14 74.7
MS.gene066580.t1 AT1G30620 27.128 188 133 3 103 288 73 258 1.37e-13 71.6

Find 116 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGTTCTAGTTCTGTTTA+TGG 0.169613 4.2:+78687970 MS.gene066580:CDS
GTTCTATCCCTTTCAGAAAA+AGG 0.194999 4.2:-78688009 None:intergenic
ATCTATGGTCTTTCTTTAAC+TGG 0.228130 4.2:+78688102 MS.gene066580:CDS
ATCCTCAACCTTCAATTGTT+TGG 0.242672 4.2:+78687947 MS.gene066580:CDS
CTCCGGCGAAGCTTTGTCTT+TGG 0.247408 4.2:+78687407 MS.gene066580:CDS
TATCTGAATTACTATTCTTC+TGG 0.256882 4.2:+78688579 MS.gene066580:CDS
TAGTGATCTTGTGGGTATTT+TGG 0.296077 4.2:+78688398 MS.gene066580:CDS
TTCTCCATCTTGAACTTTCC+CGG 0.301644 4.2:-78687349 None:intergenic
TCCTCAACCTTCAATTGTTT+GGG 0.302294 4.2:+78687948 MS.gene066580:CDS
TTTCACTGTTTATGGTCCTT+GGG 0.318056 4.2:+78688134 MS.gene066580:CDS
AAATGCATGACATGAGTAAA+AGG 0.326266 4.2:-78687820 None:intergenic
TCCTTGGGGTAGGCCTGATA+TGG 0.329537 4.2:+78688149 MS.gene066580:CDS
CAAACTGGGGTGGTCCCGTT+TGG 0.334864 4.2:+78687530 MS.gene066580:CDS
GCGTGCAAGACAAGCACTTT+TGG 0.348677 4.2:+78687723 MS.gene066580:CDS
GTCTTTCTTTAACTGGTTTG+AGG 0.353381 4.2:+78688109 MS.gene066580:CDS
TATGTTCATAGTAACATTGC+TGG 0.365279 4.2:+78687889 MS.gene066580:CDS
CATTGAGGAGTCCTTCAAAC+TGG 0.372195 4.2:+78687515 MS.gene066580:CDS
CATACTTATAATCATATCTA+TGG 0.374841 4.2:+78688087 MS.gene066580:CDS
CTCTTCCGGCGGACCCTTCA+CGG 0.384471 4.2:+78687488 MS.gene066580:CDS
AAACTGGGGTGGTCCCGTTT+GGG 0.395396 4.2:+78687531 MS.gene066580:CDS
TTATGAAGTTGCCGAGGAAT+GGG 0.400306 4.2:+78688454 MS.gene066580:CDS
ATCTGAATTACTATTCTTCT+GGG 0.411188 4.2:+78688580 MS.gene066580:CDS
CTGTTTATGGTCCTTGGGGT+AGG 0.421551 4.2:+78688139 MS.gene066580:CDS
CTGGAAGTGGTGGGAAGAAG+AGG 0.431142 4.2:+78688322 MS.gene066580:CDS
TCAATGATGCTGCTTTGTTA+AGG 0.431669 4.2:+78687779 MS.gene066580:CDS
GTTCTTGTGACCGGAGCTGC+TGG 0.437389 4.2:+78687604 MS.gene066580:CDS
TCCGGCGACTTCTCCTCTTC+CGG 0.441112 4.2:+78687474 MS.gene066580:CDS
GGAGAGGCTGTTGAAGACTA+AGG 0.452325 4.2:+78688419 MS.gene066580:CDS
TGTACGGTGGCACGCTTCTC+CGG 0.452864 4.2:+78687390 MS.gene066580:CDS
GAACTAGCCCAAACAATTGA+AGG 0.455646 4.2:-78687955 None:intergenic
TGTCTTGCACGCTTCAAAGA+AGG 0.457699 4.2:-78687712 None:intergenic
ATACCCACAAGATCACTAAC+AGG 0.465851 4.2:-78688393 None:intergenic
AACAGTGCTCCGTCGACGCC+GGG 0.468408 4.2:+78687331 MS.gene066580:CDS
ATTATGAAGTTGCCGAGGAA+TGG 0.468878 4.2:+78688453 MS.gene066580:CDS
TGTTTATGGATTGAATACTA+AGG 0.472188 4.2:+78687984 MS.gene066580:CDS
ATGCATTTGGCTGCTCAAGC+TGG 0.474694 4.2:+78687835 MS.gene066580:CDS
ACAAGATCACTAACAGGAAC+AGG 0.475033 4.2:-78688387 None:intergenic
CGTGAAGGGTCCGCCGGAAG+AGG 0.475778 4.2:-78687487 None:intergenic
TCCGACGAAACCAGCAGCTC+CGG 0.477531 4.2:-78687614 None:intergenic
GCGCTTAAACGCCGCGGAGA+CGG 0.482259 4.2:+78687652 MS.gene066580:CDS
GTTCCTGTTAGTGATCTTGT+GGG 0.482574 4.2:+78688390 MS.gene066580:CDS
CGGAGAATCCGTTCCGTGAC+CGG 0.489325 4.2:-78687582 None:intergenic
TCCGTTCCTCCGCTAGAGTC+CGG 0.497044 4.2:+78687563 MS.gene066580:CDS
CTCCAAAGACAAAGCTTCGC+CGG 0.498173 4.2:-78687409 None:intergenic
AGTTATGCGCAGAGAGAGCT+TGG 0.500038 4.2:+78688504 MS.gene066580:CDS
CGGACACGTTTCTCCCAAAC+GGG 0.500537 4.2:-78687544 None:intergenic
GATACTGCTGAGAAGAGTAC+TGG 0.501421 4.2:+78688303 MS.gene066580:CDS
CTTCTTACTTCAACCGTGTA+CGG 0.502910 4.2:+78687374 MS.gene066580:CDS
ACGGGACCACCCCAGTTTGA+AGG 0.504384 4.2:-78687526 None:intergenic
GAGAGAGCTTGGTTATAAAC+CGG 0.506070 4.2:+78688515 MS.gene066580:CDS
GGATTCTCCGTTCTTGTGAC+CGG 0.510300 4.2:+78687595 MS.gene066580:CDS
TAAACCGGTGACGGATCTGC+AGG 0.513367 4.2:+78688530 MS.gene066580:CDS
GACCGCCGTGAAGGGTCCGC+CGG 0.515234 4.2:-78687493 None:intergenic
TGTTCCTGTTAGTGATCTTG+TGG 0.519953 4.2:+78688389 MS.gene066580:CDS
ACGGACACGTTTCTCCCAAA+CGG 0.521018 4.2:-78687545 None:intergenic
AGTATGCGCGATTTCTTCAC+CGG 0.527758 4.2:-78688070 None:intergenic
CTCCTCAATGACCGCCGTGA+AGG 0.532413 4.2:-78687502 None:intergenic
GCCCAAACAATTGAAGGTTG+AGG 0.535698 4.2:-78687949 None:intergenic
ACCGGAGCTGCTGGTTTCGT+CGG 0.536422 4.2:+78687613 MS.gene066580:CDS
TGGAAGTGGTGGGAAGAAGA+GGG 0.543352 4.2:+78688323 MS.gene066580:CDS
TTCACTGTTTATGGTCCTTG+GGG 0.546844 4.2:+78688135 MS.gene066580:CDS
ACCCTTCACGGCGGTCATTG+AGG 0.548511 4.2:+78687500 MS.gene066580:CDS
ATTGAGGAGTCCTTCAAACT+GGG 0.549385 4.2:+78687516 MS.gene066580:CDS
TTATATTGATGATATTGTGA+GGG 0.550359 4.2:+78688263 MS.gene066580:CDS
GAGGAGAAGTCGCCGGAGAA+CGG 0.554120 4.2:-78687468 None:intergenic
AACTGCACATCCCCATTCCT+CGG 0.554769 4.2:-78688465 None:intergenic
TCCTCAATGACCGCCGTGAA+GGG 0.559853 4.2:-78687501 None:intergenic
TAACAGTGCTCCGTCGACGC+CGG 0.562127 4.2:+78687330 MS.gene066580:CDS
GTAGCGGCATACAAACTAGC+AGG 0.564565 4.2:-78688039 None:intergenic
TTGAACTTTCCCGGCGTCGA+CGG 0.565515 4.2:-78687340 None:intergenic
TTCACCGGCTTTCTTAGTAG+CGG 0.567698 4.2:-78688055 None:intergenic
GAGAAGCGTGCCACCGTACA+CGG 0.567964 4.2:-78687387 None:intergenic
AGCAGCTCCGGTCACAAGAA+CGG 0.569637 4.2:-78687602 None:intergenic
TTGAAAACCCTCAACTGCGC+CGG 0.571733 4.2:-78688348 None:intergenic
CCGGTGACGGATCTGCAGGC+TGG 0.573224 4.2:+78688534 MS.gene066580:CDS
ACCGGACTCTAGCGGAGGAA+CGG 0.574251 4.2:-78687564 None:intergenic
CTTATATTGATGATATTGTG+AGG 0.578974 4.2:+78688262 MS.gene066580:CDS
GAAGTGGTGGGAAGAAGAGG+GGG 0.582983 4.2:+78688325 MS.gene066580:CDS
CGAATCATGGTACCGTTGCG+AGG 0.585042 4.2:+78688232 MS.gene066580:CDS
AGCTGGTGTGAGGTATGCTA+TGG 0.586552 4.2:+78687852 MS.gene066580:CDS
AGGTATGCTATGGAGAATCC+TGG 0.586560 4.2:+78687862 MS.gene066580:CDS
CCAGCCTGCAGATCCGTCAC+CGG 0.597830 4.2:-78688534 None:intergenic
GGCGACTTCTCCTCTTCCGG+CGG 0.605382 4.2:+78687477 MS.gene066580:CDS
GGAAGTGGTGGGAAGAAGAG+GGG 0.607007 4.2:+78688324 MS.gene066580:CDS
CCTCCGCTAGAGTCCGGTCA+CGG 0.608900 4.2:+78687569 MS.gene066580:CDS
TATGAAGTTGCCGAGGAATG+GGG 0.609964 4.2:+78688455 MS.gene066580:CDS
GCTAGAGTCCGGTCACGGAA+CGG 0.610673 4.2:+78687574 MS.gene066580:CDS
GAATCATGGTACCGTTGCGA+GGG 0.611200 4.2:+78688233 MS.gene066580:CDS
GCCGGAAGAGGAGAAGTCGC+CGG 0.613076 4.2:-78687475 None:intergenic
TATGCCGCTACTAAGAAAGC+CGG 0.617216 4.2:+78688051 MS.gene066580:CDS
GCTTGGTTATAAACCGGTGA+CGG 0.619019 4.2:+78688521 MS.gene066580:CDS
GAGAAGAGTACTGGAAGTGG+TGG 0.623154 4.2:+78688312 MS.gene066580:CDS
GTTTGAAGAAATTTGTGAGG+TGG 0.624134 4.2:+78688556 MS.gene066580:CDS
TGGCTGCTCAAGCTGGTGTG+AGG 0.625330 4.2:+78687842 MS.gene066580:CDS
GCCATATCAGGCCTACCCCA+AGG 0.630669 4.2:-78688150 None:intergenic
GCTGAGAAGAGTACTGGAAG+TGG 0.633054 4.2:+78688309 MS.gene066580:CDS
GACGCCGGGAAAGTTCAAGA+TGG 0.634115 4.2:+78687345 MS.gene066580:CDS
TCCGCGGCGCTTAAACGCCG+CGG 0.635349 4.2:+78687646 MS.gene066580:CDS
TCCGCGGCGTTTAAGCGCCG+CGG 0.637292 4.2:-78687647 None:intergenic
CTGGTTTGAAGAAATTTGTG+AGG 0.647230 4.2:+78688553 MS.gene066580:CDS
AGGAGTCCTTCAAACTGGGG+TGG 0.647390 4.2:+78687520 MS.gene066580:CDS
ATAAGTAAAATCCCTCGCAA+CGG 0.649166 4.2:-78688244 None:intergenic
TCCCTAAAACACCGTCTCCG+CGG 0.649971 4.2:-78687663 None:intergenic
AGAAGAGTACTGGAAGTGGT+GGG 0.649979 4.2:+78688313 MS.gene066580:CDS
GTTCCGTGACCGGACTCTAG+CGG 0.650330 4.2:-78687572 None:intergenic
GGAATATTATGAAGTTGCCG+AGG 0.656687 4.2:+78688448 MS.gene066580:CDS
CTTACTTCAACCGTGTACGG+TGG 0.659507 4.2:+78687377 MS.gene066580:CDS
CCGTGACCGGACTCTAGCGG+AGG 0.659987 4.2:-78687569 None:intergenic
TATATTGATGATATTGTGAG+GGG 0.666488 4.2:+78688264 MS.gene066580:CDS
ATCACTAACAGGAACAGGCG+AGG 0.666779 4.2:-78688382 None:intergenic
TGTTGAAGACTAAGGCGAAG+AGG 0.671437 4.2:+78688427 MS.gene066580:CDS
TTACTATGAACATAAGAACC+AGG 0.683714 4.2:-78687880 None:intergenic
TTGAGGAGTCCTTCAAACTG+GGG 0.690015 4.2:+78687517 MS.gene066580:CDS
CGTCGGAACACATGTCTCCG+CGG 0.690793 4.2:+78687630 MS.gene066580:CDS
TTCCGGCGGACCCTTCACGG+CGG 0.735183 4.2:+78687491 MS.gene066580:CDS
ATATTGATGATATTGTGAGG+GGG 0.741076 4.2:+78688265 MS.gene066580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATACTTATAATCATATCTA+TGG + chr4.2:78688087-78688106 MS.gene066580:CDS 20.0%
!!! TTATATTGATGATATTGTGA+GGG + chr4.2:78688263-78688282 MS.gene066580:CDS 20.0%
! AAAAAGAAATAAGCCATATC+AGG - chr4.2:78688165-78688184 None:intergenic 25.0%
! ATCTGAATTACTATTCTTCT+GGG + chr4.2:78688580-78688599 MS.gene066580:CDS 25.0%
! TATCTGAATTACTATTCTTC+TGG + chr4.2:78688579-78688598 MS.gene066580:CDS 25.0%
!! CTTATATTGATGATATTGTG+AGG + chr4.2:78688262-78688281 MS.gene066580:CDS 25.0%
!! TATATTGATGATATTGTGAG+GGG + chr4.2:78688264-78688283 MS.gene066580:CDS 25.0%
!! TGTTTATGGATTGAATACTA+AGG + chr4.2:78687984-78688003 MS.gene066580:CDS 25.0%
!!! TTCACTAGAGATATTTTGAA+GGG + chr4.2:78688183-78688202 MS.gene066580:CDS 25.0%
!!! TTTCACTAGAGATATTTTGA+AGG + chr4.2:78688182-78688201 MS.gene066580:CDS 25.0%
AAATGCATGACATGAGTAAA+AGG - chr4.2:78687823-78687842 None:intergenic 30.0%
ATCTATGGTCTTTCTTTAAC+TGG + chr4.2:78688102-78688121 MS.gene066580:CDS 30.0%
TATGTTCATAGTAACATTGC+TGG + chr4.2:78687889-78687908 MS.gene066580:CDS 30.0%
TTACTATGAACATAAGAACC+AGG - chr4.2:78687883-78687902 None:intergenic 30.0%
! ATATTGATGATATTGTGAGG+GGG + chr4.2:78688265-78688284 MS.gene066580:CDS 30.0%
! TTGAGGTTTTTCACTGTTTA+TGG + chr4.2:78688126-78688145 MS.gene066580:CDS 30.0%
! TTTGTTAAGGAAGCTTTTTG+AGG + chr4.2:78687792-78687811 MS.gene066580:CDS 30.0%
! TTTTACTCATGTCATGCATT+TGG + chr4.2:78687822-78687841 MS.gene066580:CDS 30.0%
!! TAAGGTTCCTTTTTCTGAAA+GGG + chr4.2:78688002-78688021 MS.gene066580:CDS 30.0%
!! TCACTAGAGATATTTTGAAG+GGG + chr4.2:78688184-78688203 MS.gene066580:CDS 30.0%
!!! GAAGACTATTCCTATTTTTG+AGG + chr4.2:78688206-78688225 MS.gene066580:CDS 30.0%
!!! TGGTTTTGTTAATTTGCTTG+AGG + chr4.2:78687909-78687928 MS.gene066580:CDS 30.0%
ATAAGTAAAATCCCTCGCAA+CGG - chr4.2:78688247-78688266 None:intergenic 35.0%
ATCCTCAACCTTCAATTGTT+TGG + chr4.2:78687947-78687966 MS.gene066580:CDS 35.0%
GTTCTATCCCTTTCAGAAAA+AGG - chr4.2:78688012-78688031 None:intergenic 35.0%
GTTTGAAGAAATTTGTGAGG+TGG + chr4.2:78688556-78688575 MS.gene066580:CDS 35.0%
TCCTCAACCTTCAATTGTTT+GGG + chr4.2:78687948-78687967 MS.gene066580:CDS 35.0%
TTTCACTGTTTATGGTCCTT+GGG + chr4.2:78688134-78688153 MS.gene066580:CDS 35.0%
! ACTGGTGTTTTCATTGTTGA+AGG + chr4.2:78687751-78687770 MS.gene066580:CDS 35.0%
! AGTTGAGGGTTTTCAATTTG+GGG + chr4.2:78688355-78688374 MS.gene066580:CDS 35.0%
! CAGTTGAGGGTTTTCAATTT+GGG + chr4.2:78688354-78688373 MS.gene066580:CDS 35.0%
! CTAAGGTTCCTTTTTCTGAA+AGG + chr4.2:78688001-78688020 MS.gene066580:CDS 35.0%
! CTGGTTTGAAGAAATTTGTG+AGG + chr4.2:78688553-78688572 MS.gene066580:CDS 35.0%
! TAGTGATCTTGTGGGTATTT+TGG + chr4.2:78688398-78688417 MS.gene066580:CDS 35.0%
! TTTTCACTGTTTATGGTCCT+TGG + chr4.2:78688133-78688152 MS.gene066580:CDS 35.0%
!! GCTAGTTCTAGTTCTGTTTA+TGG + chr4.2:78687970-78687989 MS.gene066580:CDS 35.0%
!! GTCTTTCTTTAACTGGTTTG+AGG + chr4.2:78688109-78688128 MS.gene066580:CDS 35.0%
!! TCAATGATGCTGCTTTGTTA+AGG + chr4.2:78687779-78687798 MS.gene066580:CDS 35.0%
!!! GACTATTCCTATTTTTGAGG+CGG + chr4.2:78688209-78688228 MS.gene066580:CDS 35.0%
ACAAGATCACTAACAGGAAC+AGG - chr4.2:78688390-78688409 None:intergenic 40.0%
ATACCCACAAGATCACTAAC+AGG - chr4.2:78688396-78688415 None:intergenic 40.0%
ATTATGAAGTTGCCGAGGAA+TGG + chr4.2:78688453-78688472 MS.gene066580:CDS 40.0%
ATTGAGGAGTCCTTCAAACT+GGG + chr4.2:78687516-78687535 MS.gene066580:CDS 40.0%
CTTCTTACTTCAACCGTGTA+CGG + chr4.2:78687374-78687393 MS.gene066580:CDS 40.0%
GAACTAGCCCAAACAATTGA+AGG - chr4.2:78687958-78687977 None:intergenic 40.0%
GAGAGAGCTTGGTTATAAAC+CGG + chr4.2:78688515-78688534 MS.gene066580:CDS 40.0%
GGAATATTATGAAGTTGCCG+AGG + chr4.2:78688448-78688467 MS.gene066580:CDS 40.0%
GTTCCTGTTAGTGATCTTGT+GGG + chr4.2:78688390-78688409 MS.gene066580:CDS 40.0%
TGTTCCTGTTAGTGATCTTG+TGG + chr4.2:78688389-78688408 MS.gene066580:CDS 40.0%
TTATGAAGTTGCCGAGGAAT+GGG + chr4.2:78688454-78688473 MS.gene066580:CDS 40.0%
TTCACTGTTTATGGTCCTTG+GGG + chr4.2:78688135-78688154 MS.gene066580:CDS 40.0%
TTCTCCATCTTGAACTTTCC+CGG - chr4.2:78687352-78687371 None:intergenic 40.0%
! GATCTTCTTTTTCCGTTCTC+CGG + chr4.2:78687456-78687475 MS.gene066580:CDS 40.0%
! GCAGTTGAGGGTTTTCAATT+TGG + chr4.2:78688353-78688372 MS.gene066580:CDS 40.0%
!! ATCTTGTGGGTATTTTGGAG+AGG + chr4.2:78688403-78688422 MS.gene066580:CDS 40.0%
AGGTATGCTATGGAGAATCC+TGG + chr4.2:78687862-78687881 MS.gene066580:CDS 45.0%
AGTATGCGCGATTTCTTCAC+CGG - chr4.2:78688073-78688092 None:intergenic 45.0%
CATTGAGGAGTCCTTCAAAC+TGG + chr4.2:78687515-78687534 MS.gene066580:CDS 45.0%
GCCCAAACAATTGAAGGTTG+AGG - chr4.2:78687952-78687971 None:intergenic 45.0%
GCTTGGTTATAAACCGGTGA+CGG + chr4.2:78688521-78688540 MS.gene066580:CDS 45.0%
TATGAAGTTGCCGAGGAATG+GGG + chr4.2:78688455-78688474 MS.gene066580:CDS 45.0%
TATGCCGCTACTAAGAAAGC+CGG + chr4.2:78688051-78688070 MS.gene066580:CDS 45.0%
TGATTCGCCGCCTCAAAAAT+AGG - chr4.2:78688219-78688238 None:intergenic 45.0%
TGTCTTGCACGCTTCAAAGA+AGG - chr4.2:78687715-78687734 None:intergenic 45.0%
TGTTGAAGACTAAGGCGAAG+AGG + chr4.2:78688427-78688446 MS.gene066580:CDS 45.0%
TTGAGGAGTCCTTCAAACTG+GGG + chr4.2:78687517-78687536 MS.gene066580:CDS 45.0%
! GATACTGCTGAGAAGAGTAC+TGG + chr4.2:78688303-78688322 MS.gene066580:CDS 45.0%
!! AGAAGAGTACTGGAAGTGGT+GGG + chr4.2:78688313-78688332 MS.gene066580:CDS 45.0%
!! GATATTGTGAGGGGGTGTTT+AGG + chr4.2:78688273-78688292 MS.gene066580:CDS 45.0%
!! TTCACCGGCTTTCTTAGTAG+CGG - chr4.2:78688058-78688077 None:intergenic 45.0%
!!! ATTTTTGAGGCGGCGAATCA+TGG + chr4.2:78688219-78688238 MS.gene066580:CDS 45.0%
!!! CAAGCACTTTTGGAACGTAC+TGG + chr4.2:78687733-78687752 MS.gene066580:CDS 45.0%
AACTGCACATCCCCATTCCT+CGG - chr4.2:78688468-78688487 None:intergenic 50.0%
ACGGACACGTTTCTCCCAAA+CGG - chr4.2:78687548-78687567 None:intergenic 50.0%
AGCTGGTGTGAGGTATGCTA+TGG + chr4.2:78687852-78687871 MS.gene066580:CDS 50.0%
AGTTATGCGCAGAGAGAGCT+TGG + chr4.2:78688504-78688523 MS.gene066580:CDS 50.0%
ATCACTAACAGGAACAGGCG+AGG - chr4.2:78688385-78688404 None:intergenic 50.0%
ATGCATTTGGCTGCTCAAGC+TGG + chr4.2:78687835-78687854 MS.gene066580:CDS 50.0%
CTCCAAAGACAAAGCTTCGC+CGG - chr4.2:78687412-78687431 None:intergenic 50.0%
CTGTTTATGGTCCTTGGGGT+AGG + chr4.2:78688139-78688158 MS.gene066580:CDS 50.0%
CTTACTTCAACCGTGTACGG+TGG + chr4.2:78687377-78687396 MS.gene066580:CDS 50.0%
GAATCATGGTACCGTTGCGA+GGG + chr4.2:78688233-78688252 MS.gene066580:CDS 50.0%
GGAGAGGCTGTTGAAGACTA+AGG + chr4.2:78688419-78688438 MS.gene066580:CDS 50.0%
GGATTCTCCGTTCTTGTGAC+CGG + chr4.2:78687595-78687614 MS.gene066580:CDS 50.0%
GTAGCGGCATACAAACTAGC+AGG - chr4.2:78688042-78688061 None:intergenic 50.0%
TTGAAAACCCTCAACTGCGC+CGG - chr4.2:78688351-78688370 None:intergenic 50.0%
! GCGTGCAAGACAAGCACTTT+TGG + chr4.2:78687723-78687742 MS.gene066580:CDS 50.0%
! TGGAAGTGGTGGGAAGAAGA+GGG + chr4.2:78688323-78688342 MS.gene066580:CDS 50.0%
!! GAGAAGAGTACTGGAAGTGG+TGG + chr4.2:78688312-78688331 MS.gene066580:CDS 50.0%
!! GCTGAGAAGAGTACTGGAAG+TGG + chr4.2:78688309-78688328 MS.gene066580:CDS 50.0%
AAACTGGGGTGGTCCCGTTT+GGG + chr4.2:78687531-78687550 MS.gene066580:CDS 55.0%
AGCAGCTCCGGTCACAAGAA+CGG - chr4.2:78687605-78687624 None:intergenic 55.0%
AGGAGTCCTTCAAACTGGGG+TGG + chr4.2:78687520-78687539 MS.gene066580:CDS 55.0%
CGAATCATGGTACCGTTGCG+AGG + chr4.2:78688232-78688251 MS.gene066580:CDS 55.0%
CGGACACGTTTCTCCCAAAC+GGG - chr4.2:78687547-78687566 None:intergenic 55.0%
GACGCCGGGAAAGTTCAAGA+TGG + chr4.2:78687345-78687364 MS.gene066580:CDS 55.0%
TCCCTAAAACACCGTCTCCG+CGG - chr4.2:78687666-78687685 None:intergenic 55.0%
TCCTCAATGACCGCCGTGAA+GGG - chr4.2:78687504-78687523 None:intergenic 55.0%
TCCTTGGGGTAGGCCTGATA+TGG + chr4.2:78688149-78688168 MS.gene066580:CDS 55.0%
! CTCCGGCGAAGCTTTGTCTT+TGG + chr4.2:78687407-78687426 MS.gene066580:CDS 55.0%
! CTGGAAGTGGTGGGAAGAAG+AGG + chr4.2:78688322-78688341 MS.gene066580:CDS 55.0%
! GAAGTGGTGGGAAGAAGAGG+GGG + chr4.2:78688325-78688344 MS.gene066580:CDS 55.0%
! GGAAGTGGTGGGAAGAAGAG+GGG + chr4.2:78688324-78688343 MS.gene066580:CDS 55.0%
! TTGAACTTTCCCGGCGTCGA+CGG - chr4.2:78687343-78687362 None:intergenic 55.0%
!! GAGGGGGTGTTTAGGTGCTT+TGG + chr4.2:78688281-78688300 MS.gene066580:CDS 55.0%
!! TAAACCGGTGACGGATCTGC+AGG + chr4.2:78688530-78688549 MS.gene066580:CDS 55.0%
ACCCTTCACGGCGGTCATTG+AGG + chr4.2:78687500-78687519 MS.gene066580:CDS 60.0%
ACCGGACTCTAGCGGAGGAA+CGG - chr4.2:78687567-78687586 None:intergenic 60.0%
ACGGGACCACCCCAGTTTGA+AGG - chr4.2:78687529-78687548 None:intergenic 60.0%
CAAACTGGGGTGGTCCCGTT+TGG + chr4.2:78687530-78687549 MS.gene066580:CDS 60.0%
CGGAGAATCCGTTCCGTGAC+CGG - chr4.2:78687585-78687604 None:intergenic 60.0%
CGTCGGAACACATGTCTCCG+CGG + chr4.2:78687630-78687649 MS.gene066580:CDS 60.0%
CTCCTCAATGACCGCCGTGA+AGG - chr4.2:78687505-78687524 None:intergenic 60.0%
GAGGAGAAGTCGCCGGAGAA+CGG - chr4.2:78687471-78687490 None:intergenic 60.0%
GCCATATCAGGCCTACCCCA+AGG - chr4.2:78688153-78688172 None:intergenic 60.0%
GCTAGAGTCCGGTCACGGAA+CGG + chr4.2:78687574-78687593 MS.gene066580:CDS 60.0%
GTTCCGTGACCGGACTCTAG+CGG - chr4.2:78687575-78687594 None:intergenic 60.0%
GTTCTTGTGACCGGAGCTGC+TGG + chr4.2:78687604-78687623 MS.gene066580:CDS 60.0%
TAACAGTGCTCCGTCGACGC+CGG + chr4.2:78687330-78687349 MS.gene066580:CDS 60.0%
TCCGACGAAACCAGCAGCTC+CGG - chr4.2:78687617-78687636 None:intergenic 60.0%
TCCGGCGACTTCTCCTCTTC+CGG + chr4.2:78687474-78687493 MS.gene066580:CDS 60.0%
TCCGTTCCTCCGCTAGAGTC+CGG + chr4.2:78687563-78687582 MS.gene066580:CDS 60.0%
TGGCTGCTCAAGCTGGTGTG+AGG + chr4.2:78687842-78687861 MS.gene066580:CDS 60.0%
TGTACGGTGGCACGCTTCTC+CGG + chr4.2:78687390-78687409 MS.gene066580:CDS 60.0%
! GAGAAGCGTGCCACCGTACA+CGG - chr4.2:78687390-78687409 None:intergenic 60.0%
! GCCGCGGAGACGGTGTTTTA+GGG + chr4.2:78687662-78687681 MS.gene066580:CDS 60.0%
!! ACCGGAGCTGCTGGTTTCGT+CGG + chr4.2:78687613-78687632 MS.gene066580:CDS 60.0%
AACAGTGCTCCGTCGACGCC+GGG + chr4.2:78687331-78687350 MS.gene066580:CDS 65.0%
CCAGCCTGCAGATCCGTCAC+CGG - chr4.2:78688537-78688556 None:intergenic 65.0%
CCTCCGCTAGAGTCCGGTCA+CGG + chr4.2:78687569-78687588 MS.gene066580:CDS 65.0%
CTCTTCCGGCGGACCCTTCA+CGG + chr4.2:78687488-78687507 MS.gene066580:CDS 65.0%
GCCGGAAGAGGAGAAGTCGC+CGG - chr4.2:78687478-78687497 None:intergenic 65.0%
GCGCTTAAACGCCGCGGAGA+CGG + chr4.2:78687652-78687671 MS.gene066580:CDS 65.0%
GGCGACTTCTCCTCTTCCGG+CGG + chr4.2:78687477-78687496 MS.gene066580:CDS 65.0%
TGGTGGGAAGAAGAGGGGGC+CGG + chr4.2:78688329-78688348 MS.gene066580:CDS 65.0%
! CGCCGCGGAGACGGTGTTTT+AGG + chr4.2:78687661-78687680 MS.gene066580:CDS 65.0%
CCGTGACCGGACTCTAGCGG+AGG - chr4.2:78687572-78687591 None:intergenic 70.0%
CGTGAAGGGTCCGCCGGAAG+AGG - chr4.2:78687490-78687509 None:intergenic 70.0%
TCCGCGGCGCTTAAACGCCG+CGG + chr4.2:78687646-78687665 MS.gene066580:CDS 70.0%
TTCCGGCGGACCCTTCACGG+CGG + chr4.2:78687491-78687510 MS.gene066580:CDS 70.0%
! TCCGCGGCGTTTAAGCGCCG+CGG - chr4.2:78687650-78687669 None:intergenic 70.0%
!! CCGGTGACGGATCTGCAGGC+TGG + chr4.2:78688534-78688553 MS.gene066580:CDS 70.0%
AGAGGGGGCCGGCGCAGTTG+AGG + chr4.2:78688340-78688359 MS.gene066580:CDS 75.0%
GACCGCCGTGAAGGGTCCGC+CGG - chr4.2:78687496-78687515 None:intergenic 75.0%
GAGGGGGCCGGCGCAGTTGA+GGG + chr4.2:78688341-78688360 MS.gene066580:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 78687298 78688620 78687298 ID=MS.gene066580
chr4.2 mRNA 78687298 78688620 78687298 ID=MS.gene066580.t1;Parent=MS.gene066580
chr4.2 exon 78687298 78688620 78687298 ID=MS.gene066580.t1.exon1;Parent=MS.gene066580.t1
chr4.2 CDS 78687298 78688620 78687298 ID=cds.MS.gene066580.t1;Parent=MS.gene066580.t1
Gene Sequence

>MS.gene066580

ATGTCTCAGCTGAAGCAAATGTCGCACGCTGATAACAGTGCTCCGTCGACGCCGGGAAAGTTCAAGATGGAGAAAGCTTCTTACTTCAACCGTGTACGGTGGCACGCTTCTCCGGCGAAGCTTTGTCTTTGGAGTTTTGTTTTCTCAGCTGCGATCTTGATCTTCTTTTTCCGTTCTCCGGCGACTTCTCCTCTTCCGGCGGACCCTTCACGGCGGTCATTGAGGAGTCCTTCAAACTGGGGTGGTCCCGTTTGGGAGAAACGTGTCCGTTCCTCCGCTAGAGTCCGGTCACGGAACGGATTCTCCGTTCTTGTGACCGGAGCTGCTGGTTTCGTCGGAACACATGTCTCCGCGGCGCTTAAACGCCGCGGAGACGGTGTTTTAGGGATTGATAATTTCAATGACTATTATGATCCTTCTTTGAAGCGTGCAAGACAAGCACTTTTGGAACGTACTGGTGTTTTCATTGTTGAAGGTGATATCAATGATGCTGCTTTGTTAAGGAAGCTTTTTGAGGTTGTTCCTTTTACTCATGTCATGCATTTGGCTGCTCAAGCTGGTGTGAGGTATGCTATGGAGAATCCTGGTTCTTATGTTCATAGTAACATTGCTGGTTTTGTTAATTTGCTTGAGGTTTGTAAAAGTGTTAATCCTCAACCTTCAATTGTTTGGGCTAGTTCTAGTTCTGTTTATGGATTGAATACTAAGGTTCCTTTTTCTGAAAGGGATAGAACTGATCAACCTGCTAGTTTGTATGCCGCTACTAAGAAAGCCGGTGAAGAAATCGCGCATACTTATAATCATATCTATGGTCTTTCTTTAACTGGTTTGAGGTTTTTCACTGTTTATGGTCCTTGGGGTAGGCCTGATATGGCTTATTTCTTTTTCACTAGAGATATTTTGAAGGGGAAGACTATTCCTATTTTTGAGGCGGCGAATCATGGTACCGTTGCGAGGGATTTTACTTATATTGATGATATTGTGAGGGGGTGTTTAGGTGCTTTGGATACTGCTGAGAAGAGTACTGGAAGTGGTGGGAAGAAGAGGGGGCCGGCGCAGTTGAGGGTTTTCAATTTGGGGAATACCTCGCCTGTTCCTGTTAGTGATCTTGTGGGTATTTTGGAGAGGCTGTTGAAGACTAAGGCGAAGAGGAATATTATGAAGTTGCCGAGGAATGGGGATGTGCAGTTTACTCATGCGAATATTAGTTATGCGCAGAGAGAGCTTGGTTATAAACCGGTGACGGATCTGCAGGCTGGTTTGAAGAAATTTGTGAGGTGGTATCTGAATTACTATTCTTCTGGGAAGAAAGCTGTTGAGTGA
Protein sequence

>MS.gene066580.t1

MSQLKQMSHADNSAPSTPGKFKMEKASYFNRVRWHASPAKLCLWSFVFSAAILIFFFRSPATSPLPADPSRRSLRSPSNWGGPVWEKRVRSSARVRSRNGFSVLVTGAAGFVGTHVSAALKRRGDGVLGIDNFNDYYDPSLKRARQALLERTGVFIVEGDINDAALLRKLFEVVPFTHVMHLAAQAGVRYAMENPGSYVHSNIAGFVNLLEVCKSVNPQPSIVWASSSSVYGLNTKVPFSERDRTDQPASLYAATKKAGEEIAHTYNHIYGLSLTGLRFFTVYGPWGRPDMAYFFFTRDILKGKTIPIFEAANHGTVARDFTYIDDIVRGCLGALDTAEKSTGSGGKKRGPAQLRVFNLGNTSPVPVSDLVGILERLLKTKAKRNIMKLPRNGDVQFTHANISYAQRELGYKPVTDLQAGLKKFVRWYLNYYSSGKKAVE