AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48984

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48984.t1 MTR_4g024600 99.773 440 1 0 1 440 1 440 0.0 907
MS.gene48984.t1 MTR_0354s0040 82.629 426 66 3 15 440 4 421 0.0 728
MS.gene48984.t1 MTR_4g102270 69.431 422 125 3 11 432 7 424 0.0 603
MS.gene48984.t1 MTR_4g129820 67.431 436 132 4 8 440 6 434 0.0 593
MS.gene48984.t1 MTR_3g084090 66.047 430 134 3 13 432 3 430 0.0 585
MS.gene48984.t1 MTR_5g011940 76.294 367 85 1 69 433 56 422 0.0 581
MS.gene48984.t1 MTR_0034s0060 27.545 334 213 8 102 428 138 449 9.11e-22 98.6
MS.gene48984.t1 MTR_8g096880 28.614 332 199 12 103 412 8 323 1.45e-21 95.9
MS.gene48984.t1 MTR_8g096880 29.042 334 195 13 103 412 8 323 2.08e-21 94.7
MS.gene48984.t1 MTR_2g073240 26.822 343 208 8 102 430 20 333 2.62e-20 94.0
MS.gene48984.t1 MTR_2g032100 26.087 345 219 12 103 427 73 401 2.65e-20 92.8
MS.gene48984.t1 MTR_5g009170 26.039 361 230 12 91 433 38 379 3.38e-20 92.4
MS.gene48984.t1 MTR_8g099535 27.576 330 205 12 102 412 5 319 7.92e-20 90.9
MS.gene48984.t1 MTR_1g009600 25.519 337 220 10 102 430 6 319 3.17e-19 90.9
MS.gene48984.t1 MTR_4g120030 25.397 441 251 17 8 425 20 405 6.54e-19 89.0
MS.gene48984.t1 MTR_5g009160 26.923 338 203 11 99 412 2 319 1.07e-18 87.4
MS.gene48984.t1 MTR_1g088480 25.816 337 202 12 98 425 115 412 1.20e-18 88.2
MS.gene48984.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene48984.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene48984.t1 MTR_4g063600 24.471 331 212 11 98 421 29 328 2.91e-17 83.2
MS.gene48984.t1 MTR_8g096880 28.041 296 176 11 103 376 8 288 8.39e-17 81.3
MS.gene48984.t1 MTR_4g063600 24.773 331 208 12 98 421 29 325 2.90e-16 80.1
MS.gene48984.t1 MTR_7g115080 25.000 336 210 13 103 427 31 335 2.48e-15 77.8
MS.gene48984.t1 MTR_7g115080 25.000 336 210 13 103 427 65 369 2.64e-15 77.8
MS.gene48984.t1 MTR_7g115080 25.000 336 210 13 103 427 44 348 2.70e-15 77.8
MS.gene48984.t1 MTR_1g080950 23.810 399 247 16 41 427 18 371 4.46e-15 77.0
MS.gene48984.t1 MTR_2g032100 29.630 189 127 5 103 288 73 258 6.25e-15 75.5
MS.gene48984.t1 MTR_1g080950 24.189 339 214 12 101 427 28 335 8.07e-15 76.3
MS.gene48984.t1 MTR_2g096660 24.324 333 210 12 98 421 35 334 1.67e-14 75.1
MS.gene48984.t1 MTR_4g101940 86.667 30 4 0 173 202 42 71 2.71e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48984.t1 AT4G00110 86.667 435 51 4 4 438 1 428 0.0 770
MS.gene48984.t1 AT1G02000 84.828 435 59 5 7 439 1 430 0.0 751
MS.gene48984.t1 AT2G45310 78.851 435 88 3 7 438 1 434 0.0 693
MS.gene48984.t1 AT4G12250 73.059 438 105 4 7 437 1 432 0.0 621
MS.gene48984.t1 AT3G23820 77.557 352 79 0 81 432 92 443 0.0 582
MS.gene48984.t1 AT4G30440 67.907 430 126 4 11 438 6 425 0.0 564
MS.gene48984.t1 AT4G23920 27.350 351 215 12 102 430 4 336 4.19e-23 100
MS.gene48984.t1 AT1G12780 28.657 335 197 13 102 412 8 324 1.10e-22 99.0
MS.gene48984.t1 AT1G63180 28.443 334 199 12 102 412 8 324 5.08e-21 94.4
MS.gene48984.t1 AT1G78570 26.647 334 216 8 102 428 8 319 8.81e-21 95.5
MS.gene48984.t1 AT3G14790 26.647 334 216 8 102 428 11 322 2.27e-20 94.4
MS.gene48984.t1 AT3G14790 26.647 334 216 8 102 428 8 319 2.54e-20 94.4
MS.gene48984.t1 AT4G10960 26.891 357 209 13 102 430 5 337 7.75e-20 90.9
MS.gene48984.t1 AT1G30620 25.507 345 221 11 103 427 72 400 4.10e-19 89.4
MS.gene48984.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene48984.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene48984.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene48984.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene48984.t1 AT1G53500 26.393 341 208 8 102 428 10 321 9.08e-19 89.4
MS.gene48984.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene48984.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene48984.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene48984.t1 AT5G44480 24.709 344 225 10 103 427 97 425 2.18e-18 87.4
MS.gene48984.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene48984.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene48984.t1 AT3G53520 25.294 340 215 10 98 430 117 424 1.40e-17 85.1
MS.gene48984.t1 AT1G64440 25.281 356 220 12 102 434 4 336 1.42e-17 84.0
MS.gene48984.t1 AT2G47650 24.122 427 252 16 32 426 35 421 3.98e-17 83.6
MS.gene48984.t1 AT4G20460 25.212 353 227 12 96 427 64 400 4.53e-17 83.2
MS.gene48984.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene48984.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene48984.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene48984.t1 AT2G28760 25.225 333 207 13 98 421 59 358 8.34e-17 82.0
MS.gene48984.t1 AT1G63180 28.188 298 175 11 102 376 8 289 9.29e-17 81.3
MS.gene48984.t1 AT3G53520 25.000 340 214 11 98 430 117 422 1.72e-15 78.6
MS.gene48984.t1 AT2G47650 24.249 433 250 18 32 426 35 427 3.98e-15 77.4
MS.gene48984.t1 AT5G59290 24.085 328 207 12 103 421 47 341 7.57e-15 76.3
MS.gene48984.t1 AT5G59290 24.085 328 207 12 103 421 32 326 8.39e-15 75.9
MS.gene48984.t1 AT5G59290 24.085 328 207 12 103 421 3 297 9.21e-15 75.5
MS.gene48984.t1 AT5G59290 24.085 328 207 12 103 421 25 319 1.03e-14 75.5
MS.gene48984.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene48984.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene48984.t1 AT3G53520 27.083 240 153 6 98 331 117 340 2.60e-14 74.3
MS.gene48984.t1 AT3G53520 24.011 354 225 12 98 430 117 447 3.55e-14 74.7
MS.gene48984.t1 AT1G30620 27.128 188 133 3 103 288 73 258 1.37e-13 71.6

Find 117 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGTTCTAGTTCTGTTTA+TGG 0.169613 4.3:+74141877 MS.gene48984:CDS
GTTCTATCCCTTTCAGAAAA+AGG 0.194999 4.3:-74141916 None:intergenic
ATCTATGGTCTTTCTTTAAC+TGG 0.228130 4.3:+74142009 MS.gene48984:CDS
ATCCTCAACCTTCAATTGTT+TGG 0.242672 4.3:+74141854 MS.gene48984:CDS
CTCCGGCGAAGCTTTGTCTT+TGG 0.247408 4.3:+74141314 MS.gene48984:CDS
TATCTGAATTACTATTCTTC+TGG 0.256882 4.3:+74142486 MS.gene48984:CDS
TAGTGATCTTGTGGGTATTT+TGG 0.296077 4.3:+74142305 MS.gene48984:CDS
TTCTCCATCTTGAACTTTCC+CGG 0.301644 4.3:-74141256 None:intergenic
TCCTCAACCTTCAATTGTTT+GGG 0.302294 4.3:+74141855 MS.gene48984:CDS
TTTCACTGTTTATGGTCCTT+GGG 0.318056 4.3:+74142041 MS.gene48984:CDS
AAATGCATGACATGAGTAAA+AGG 0.326266 4.3:-74141727 None:intergenic
TCCTTGGGGTAGGCCTGATA+TGG 0.329537 4.3:+74142056 MS.gene48984:CDS
CAAACTGGGGTGGTCCCGTT+TGG 0.334864 4.3:+74141437 MS.gene48984:CDS
GCGTGCAAGACAAGCACTTT+TGG 0.348677 4.3:+74141630 MS.gene48984:CDS
GTCTTTCTTTAACTGGTTTG+AGG 0.353381 4.3:+74142016 MS.gene48984:CDS
TATGTTCATAGTAACATTGC+TGG 0.365279 4.3:+74141796 MS.gene48984:CDS
CATTGAGGAGTCCTTCAAAC+TGG 0.372195 4.3:+74141422 MS.gene48984:CDS
CATAATATTCCTCTTTGCCT+TGG 0.373669 4.3:-74142343 None:intergenic
CATACTTATAATCATATCTA+TGG 0.374841 4.3:+74141994 MS.gene48984:CDS
CTCTTCCGGCGGACCCTTCA+CGG 0.384471 4.3:+74141395 MS.gene48984:CDS
AAACTGGGGTGGTCCCGTTT+GGG 0.395396 4.3:+74141438 MS.gene48984:CDS
TTATGAAGTTGCCGAGGAAT+GGG 0.400306 4.3:+74142361 MS.gene48984:CDS
ATCTGAATTACTATTCTTCT+GGG 0.411188 4.3:+74142487 MS.gene48984:CDS
CTGTTTATGGTCCTTGGGGT+AGG 0.421551 4.3:+74142046 MS.gene48984:CDS
CTGGAAGTGGTGGGAAGAAG+AGG 0.431142 4.3:+74142229 MS.gene48984:CDS
TCAATGATGCTGCTTTGTTA+AGG 0.431669 4.3:+74141686 MS.gene48984:CDS
GTTCTTGTGACCGGAGCTGC+TGG 0.437389 4.3:+74141511 MS.gene48984:CDS
TCCGGCGACTTCTCCTCTTC+CGG 0.441112 4.3:+74141381 MS.gene48984:CDS
TGTACGGTGGCACGCTTCTC+CGG 0.452864 4.3:+74141297 MS.gene48984:CDS
GAACTAGCCCAAACAATTGA+AGG 0.455646 4.3:-74141862 None:intergenic
TGTCTTGCACGCTTCAAAGA+AGG 0.457699 4.3:-74141619 None:intergenic
ATACCCACAAGATCACTAAC+AGG 0.465851 4.3:-74142300 None:intergenic
AACAGTGCTCCGTCGACGCC+GGG 0.468408 4.3:+74141238 MS.gene48984:CDS
ATTATGAAGTTGCCGAGGAA+TGG 0.468878 4.3:+74142360 MS.gene48984:CDS
TGTTTATGGATTGAATACTA+AGG 0.472188 4.3:+74141891 MS.gene48984:CDS
ATGCATTTGGCTGCTCAAGC+TGG 0.474694 4.3:+74141742 MS.gene48984:CDS
ACAAGATCACTAACAGGAAC+AGG 0.475033 4.3:-74142294 None:intergenic
CGTGAAGGGTCCGCCGGAAG+AGG 0.475778 4.3:-74141394 None:intergenic
TCCGACGAAACCAGCAGCTC+CGG 0.477531 4.3:-74141521 None:intergenic
GCGCTTAAACGCCGCGGAGA+CGG 0.482259 4.3:+74141559 MS.gene48984:CDS
GTTCCTGTTAGTGATCTTGT+GGG 0.482574 4.3:+74142297 MS.gene48984:CDS
CGGAGAATCCGTTCCGTGAC+CGG 0.489325 4.3:-74141489 None:intergenic
TCCGTTCCTCCGCTAGAGTC+CGG 0.497044 4.3:+74141470 MS.gene48984:CDS
CTCCAAAGACAAAGCTTCGC+CGG 0.498173 4.3:-74141316 None:intergenic
AGTTATGCGCAGAGAGAGCT+TGG 0.500038 4.3:+74142411 MS.gene48984:CDS
CGGACACGTTTCTCCCAAAC+GGG 0.500537 4.3:-74141451 None:intergenic
GATACTGCTGAGAAGAGTAC+TGG 0.501421 4.3:+74142210 MS.gene48984:CDS
CTTCTTACTTCAACCGTGTA+CGG 0.502910 4.3:+74141281 MS.gene48984:CDS
ACGGGACCACCCCAGTTTGA+AGG 0.504384 4.3:-74141433 None:intergenic
GAGAGAGCTTGGTTATAAAC+CGG 0.506070 4.3:+74142422 MS.gene48984:CDS
GGATTCTCCGTTCTTGTGAC+CGG 0.510300 4.3:+74141502 MS.gene48984:CDS
TAAACCGGTGACGGATCTGC+AGG 0.513367 4.3:+74142437 MS.gene48984:CDS
GACCGCCGTGAAGGGTCCGC+CGG 0.515234 4.3:-74141400 None:intergenic
TGTTCCTGTTAGTGATCTTG+TGG 0.519953 4.3:+74142296 MS.gene48984:CDS
ACGGACACGTTTCTCCCAAA+CGG 0.521018 4.3:-74141452 None:intergenic
AGTATGCGCGATTTCTTCAC+CGG 0.527758 4.3:-74141977 None:intergenic
CTCCTCAATGACCGCCGTGA+AGG 0.532413 4.3:-74141409 None:intergenic
GCCCAAACAATTGAAGGTTG+AGG 0.535698 4.3:-74141856 None:intergenic
ACCGGAGCTGCTGGTTTCGT+CGG 0.536422 4.3:+74141520 MS.gene48984:CDS
TGGAAGTGGTGGGAAGAAGA+GGG 0.543352 4.3:+74142230 MS.gene48984:CDS
TTCACTGTTTATGGTCCTTG+GGG 0.546844 4.3:+74142042 MS.gene48984:CDS
ACCCTTCACGGCGGTCATTG+AGG 0.548511 4.3:+74141407 MS.gene48984:CDS
ATTGAGGAGTCCTTCAAACT+GGG 0.549385 4.3:+74141423 MS.gene48984:CDS
TTATATTGATGATATTGTGA+GGG 0.550359 4.3:+74142170 MS.gene48984:CDS
GAGGAGAAGTCGCCGGAGAA+CGG 0.554120 4.3:-74141375 None:intergenic
AACTGCACATCCCCATTCCT+CGG 0.554769 4.3:-74142372 None:intergenic
TCCTCAATGACCGCCGTGAA+GGG 0.559853 4.3:-74141408 None:intergenic
TAACAGTGCTCCGTCGACGC+CGG 0.562127 4.3:+74141237 MS.gene48984:CDS
GTAGCGGCATACAAACTAGC+AGG 0.564565 4.3:-74141946 None:intergenic
TTGAACTTTCCCGGCGTCGA+CGG 0.565515 4.3:-74141247 None:intergenic
TTCACCGGCTTTCTTAGTAG+CGG 0.567698 4.3:-74141962 None:intergenic
GAGAAGCGTGCCACCGTACA+CGG 0.567964 4.3:-74141294 None:intergenic
AGCAGCTCCGGTCACAAGAA+CGG 0.569637 4.3:-74141509 None:intergenic
TTGAAAACCCTCAACTGCGC+CGG 0.571733 4.3:-74142255 None:intergenic
CCGGTGACGGATCTGCAGGC+TGG 0.573224 4.3:+74142441 MS.gene48984:CDS
ACCGGACTCTAGCGGAGGAA+CGG 0.574251 4.3:-74141471 None:intergenic
CTTATATTGATGATATTGTG+AGG 0.578974 4.3:+74142169 MS.gene48984:CDS
GAAGTGGTGGGAAGAAGAGG+GGG 0.582983 4.3:+74142232 MS.gene48984:CDS
CGAATCATGGTACCGTTGCG+AGG 0.585042 4.3:+74142139 MS.gene48984:CDS
AGCTGGTGTGAGGTATGCTA+TGG 0.586552 4.3:+74141759 MS.gene48984:CDS
AGGTATGCTATGGAGAATCC+TGG 0.586560 4.3:+74141769 MS.gene48984:CDS
GGAGAGGCTGTTGAAGACCA+AGG 0.596523 4.3:+74142326 MS.gene48984:CDS
CCAGCCTGCAGATCCGTCAC+CGG 0.597830 4.3:-74142441 None:intergenic
GGCGACTTCTCCTCTTCCGG+CGG 0.605382 4.3:+74141384 MS.gene48984:CDS
GGAAGTGGTGGGAAGAAGAG+GGG 0.607007 4.3:+74142231 MS.gene48984:CDS
CCTCCGCTAGAGTCCGGTCA+CGG 0.608900 4.3:+74141476 MS.gene48984:CDS
TATGAAGTTGCCGAGGAATG+GGG 0.609964 4.3:+74142362 MS.gene48984:CDS
GCTAGAGTCCGGTCACGGAA+CGG 0.610673 4.3:+74141481 MS.gene48984:CDS
GAATCATGGTACCGTTGCGA+GGG 0.611200 4.3:+74142140 MS.gene48984:CDS
GCCGGAAGAGGAGAAGTCGC+CGG 0.613076 4.3:-74141382 None:intergenic
TATGCCGCTACTAAGAAAGC+CGG 0.617216 4.3:+74141958 MS.gene48984:CDS
GCTTGGTTATAAACCGGTGA+CGG 0.619019 4.3:+74142428 MS.gene48984:CDS
GAGAAGAGTACTGGAAGTGG+TGG 0.623154 4.3:+74142219 MS.gene48984:CDS
GTTTGAAGAAATTTGTGAGG+TGG 0.624134 4.3:+74142463 MS.gene48984:CDS
TGGCTGCTCAAGCTGGTGTG+AGG 0.625330 4.3:+74141749 MS.gene48984:CDS
GCCATATCAGGCCTACCCCA+AGG 0.630669 4.3:-74142057 None:intergenic
GCTGAGAAGAGTACTGGAAG+TGG 0.633054 4.3:+74142216 MS.gene48984:CDS
TGTTGAAGACCAAGGCAAAG+AGG 0.633114 4.3:+74142334 MS.gene48984:CDS
GACGCCGGGAAAGTTCAAGA+TGG 0.634115 4.3:+74141252 MS.gene48984:CDS
TCCGCGGCGCTTAAACGCCG+CGG 0.635349 4.3:+74141553 MS.gene48984:CDS
TCCGCGGCGTTTAAGCGCCG+CGG 0.637292 4.3:-74141554 None:intergenic
CTGGTTTGAAGAAATTTGTG+AGG 0.647230 4.3:+74142460 MS.gene48984:CDS
AGGAGTCCTTCAAACTGGGG+TGG 0.647390 4.3:+74141427 MS.gene48984:CDS
ATAAGTAAAATCCCTCGCAA+CGG 0.649166 4.3:-74142151 None:intergenic
TCCCTAAAACACCGTCTCCG+CGG 0.649971 4.3:-74141570 None:intergenic
AGAAGAGTACTGGAAGTGGT+GGG 0.649979 4.3:+74142220 MS.gene48984:CDS
GTTCCGTGACCGGACTCTAG+CGG 0.650330 4.3:-74141479 None:intergenic
GGAATATTATGAAGTTGCCG+AGG 0.656687 4.3:+74142355 MS.gene48984:CDS
CTTACTTCAACCGTGTACGG+TGG 0.659507 4.3:+74141284 MS.gene48984:CDS
CCGTGACCGGACTCTAGCGG+AGG 0.659987 4.3:-74141476 None:intergenic
TATATTGATGATATTGTGAG+GGG 0.666488 4.3:+74142171 MS.gene48984:CDS
ATCACTAACAGGAACAGGCG+AGG 0.666779 4.3:-74142289 None:intergenic
TTACTATGAACATAAGAACC+AGG 0.683714 4.3:-74141787 None:intergenic
TTGAGGAGTCCTTCAAACTG+GGG 0.690015 4.3:+74141424 MS.gene48984:CDS
CGTCGGAACACATGTCTCCG+CGG 0.690793 4.3:+74141537 MS.gene48984:CDS
TTCCGGCGGACCCTTCACGG+CGG 0.735183 4.3:+74141398 MS.gene48984:CDS
ATATTGATGATATTGTGAGG+GGG 0.741076 4.3:+74142172 MS.gene48984:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATACTTATAATCATATCTA+TGG + chr4.3:74141994-74142013 MS.gene48984:CDS 20.0%
!!! TTATATTGATGATATTGTGA+GGG + chr4.3:74142170-74142189 MS.gene48984:CDS 20.0%
! AAAAAGAAATAAGCCATATC+AGG - chr4.3:74142072-74142091 None:intergenic 25.0%
! ATCTGAATTACTATTCTTCT+GGG + chr4.3:74142487-74142506 MS.gene48984:CDS 25.0%
! TATCTGAATTACTATTCTTC+TGG + chr4.3:74142486-74142505 MS.gene48984:CDS 25.0%
!! CTTATATTGATGATATTGTG+AGG + chr4.3:74142169-74142188 MS.gene48984:CDS 25.0%
!! TATATTGATGATATTGTGAG+GGG + chr4.3:74142171-74142190 MS.gene48984:CDS 25.0%
!! TGTTTATGGATTGAATACTA+AGG + chr4.3:74141891-74141910 MS.gene48984:CDS 25.0%
!!! TTCACTAGAGATATTTTGAA+GGG + chr4.3:74142090-74142109 MS.gene48984:CDS 25.0%
!!! TTTCACTAGAGATATTTTGA+AGG + chr4.3:74142089-74142108 MS.gene48984:CDS 25.0%
AAATGCATGACATGAGTAAA+AGG - chr4.3:74141730-74141749 None:intergenic 30.0%
ATCTATGGTCTTTCTTTAAC+TGG + chr4.3:74142009-74142028 MS.gene48984:CDS 30.0%
TATGTTCATAGTAACATTGC+TGG + chr4.3:74141796-74141815 MS.gene48984:CDS 30.0%
TTACTATGAACATAAGAACC+AGG - chr4.3:74141790-74141809 None:intergenic 30.0%
! ATATTGATGATATTGTGAGG+GGG + chr4.3:74142172-74142191 MS.gene48984:CDS 30.0%
! TTGAGGTTTTTCACTGTTTA+TGG + chr4.3:74142033-74142052 MS.gene48984:CDS 30.0%
! TTTGTTAAGGAAGCTTTTTG+AGG + chr4.3:74141699-74141718 MS.gene48984:CDS 30.0%
! TTTTACTCATGTCATGCATT+TGG + chr4.3:74141729-74141748 MS.gene48984:CDS 30.0%
!! TAAGGTTCCTTTTTCTGAAA+GGG + chr4.3:74141909-74141928 MS.gene48984:CDS 30.0%
!! TCACTAGAGATATTTTGAAG+GGG + chr4.3:74142091-74142110 MS.gene48984:CDS 30.0%
!!! GAAGACTATTCCTATTTTTG+AGG + chr4.3:74142113-74142132 MS.gene48984:CDS 30.0%
!!! TGGTTTTGTTAATTTGCTTG+AGG + chr4.3:74141816-74141835 MS.gene48984:CDS 30.0%
ATAAGTAAAATCCCTCGCAA+CGG - chr4.3:74142154-74142173 None:intergenic 35.0%
ATCCTCAACCTTCAATTGTT+TGG + chr4.3:74141854-74141873 MS.gene48984:CDS 35.0%
CATAATATTCCTCTTTGCCT+TGG - chr4.3:74142346-74142365 None:intergenic 35.0%
GTTCTATCCCTTTCAGAAAA+AGG - chr4.3:74141919-74141938 None:intergenic 35.0%
GTTTGAAGAAATTTGTGAGG+TGG + chr4.3:74142463-74142482 MS.gene48984:CDS 35.0%
TCCTCAACCTTCAATTGTTT+GGG + chr4.3:74141855-74141874 MS.gene48984:CDS 35.0%
TTTCACTGTTTATGGTCCTT+GGG + chr4.3:74142041-74142060 MS.gene48984:CDS 35.0%
! ACTGGTGTTTTCATTGTTGA+AGG + chr4.3:74141658-74141677 MS.gene48984:CDS 35.0%
! AGTTGAGGGTTTTCAATTTG+GGG + chr4.3:74142262-74142281 MS.gene48984:CDS 35.0%
! CAGTTGAGGGTTTTCAATTT+GGG + chr4.3:74142261-74142280 MS.gene48984:CDS 35.0%
! CTAAGGTTCCTTTTTCTGAA+AGG + chr4.3:74141908-74141927 MS.gene48984:CDS 35.0%
! CTGGTTTGAAGAAATTTGTG+AGG + chr4.3:74142460-74142479 MS.gene48984:CDS 35.0%
! TAGTGATCTTGTGGGTATTT+TGG + chr4.3:74142305-74142324 MS.gene48984:CDS 35.0%
! TTTTCACTGTTTATGGTCCT+TGG + chr4.3:74142040-74142059 MS.gene48984:CDS 35.0%
!! GCTAGTTCTAGTTCTGTTTA+TGG + chr4.3:74141877-74141896 MS.gene48984:CDS 35.0%
!! GTCTTTCTTTAACTGGTTTG+AGG + chr4.3:74142016-74142035 MS.gene48984:CDS 35.0%
!! TCAATGATGCTGCTTTGTTA+AGG + chr4.3:74141686-74141705 MS.gene48984:CDS 35.0%
!!! GACTATTCCTATTTTTGAGG+CGG + chr4.3:74142116-74142135 MS.gene48984:CDS 35.0%
ACAAGATCACTAACAGGAAC+AGG - chr4.3:74142297-74142316 None:intergenic 40.0%
ATACCCACAAGATCACTAAC+AGG - chr4.3:74142303-74142322 None:intergenic 40.0%
ATTATGAAGTTGCCGAGGAA+TGG + chr4.3:74142360-74142379 MS.gene48984:CDS 40.0%
ATTGAGGAGTCCTTCAAACT+GGG + chr4.3:74141423-74141442 MS.gene48984:CDS 40.0%
CTTCTTACTTCAACCGTGTA+CGG + chr4.3:74141281-74141300 MS.gene48984:CDS 40.0%
GAACTAGCCCAAACAATTGA+AGG - chr4.3:74141865-74141884 None:intergenic 40.0%
GAGAGAGCTTGGTTATAAAC+CGG + chr4.3:74142422-74142441 MS.gene48984:CDS 40.0%
GGAATATTATGAAGTTGCCG+AGG + chr4.3:74142355-74142374 MS.gene48984:CDS 40.0%
GTTCCTGTTAGTGATCTTGT+GGG + chr4.3:74142297-74142316 MS.gene48984:CDS 40.0%
TGTTCCTGTTAGTGATCTTG+TGG + chr4.3:74142296-74142315 MS.gene48984:CDS 40.0%
TTATGAAGTTGCCGAGGAAT+GGG + chr4.3:74142361-74142380 MS.gene48984:CDS 40.0%
TTCACTGTTTATGGTCCTTG+GGG + chr4.3:74142042-74142061 MS.gene48984:CDS 40.0%
TTCTCCATCTTGAACTTTCC+CGG - chr4.3:74141259-74141278 None:intergenic 40.0%
! GATCTTCTTTTTCCGTTCTC+CGG + chr4.3:74141363-74141382 MS.gene48984:CDS 40.0%
! GCAGTTGAGGGTTTTCAATT+TGG + chr4.3:74142260-74142279 MS.gene48984:CDS 40.0%
!! ATCTTGTGGGTATTTTGGAG+AGG + chr4.3:74142310-74142329 MS.gene48984:CDS 40.0%
AGGTATGCTATGGAGAATCC+TGG + chr4.3:74141769-74141788 MS.gene48984:CDS 45.0%
AGTATGCGCGATTTCTTCAC+CGG - chr4.3:74141980-74141999 None:intergenic 45.0%
CATTGAGGAGTCCTTCAAAC+TGG + chr4.3:74141422-74141441 MS.gene48984:CDS 45.0%
GCCCAAACAATTGAAGGTTG+AGG - chr4.3:74141859-74141878 None:intergenic 45.0%
GCTTGGTTATAAACCGGTGA+CGG + chr4.3:74142428-74142447 MS.gene48984:CDS 45.0%
TATGAAGTTGCCGAGGAATG+GGG + chr4.3:74142362-74142381 MS.gene48984:CDS 45.0%
TATGCCGCTACTAAGAAAGC+CGG + chr4.3:74141958-74141977 MS.gene48984:CDS 45.0%
TGATTCGCCGCCTCAAAAAT+AGG - chr4.3:74142126-74142145 None:intergenic 45.0%
TGTCTTGCACGCTTCAAAGA+AGG - chr4.3:74141622-74141641 None:intergenic 45.0%
TGTTGAAGACCAAGGCAAAG+AGG + chr4.3:74142334-74142353 MS.gene48984:CDS 45.0%
TTGAGGAGTCCTTCAAACTG+GGG + chr4.3:74141424-74141443 MS.gene48984:CDS 45.0%
! GATACTGCTGAGAAGAGTAC+TGG + chr4.3:74142210-74142229 MS.gene48984:CDS 45.0%
!! AGAAGAGTACTGGAAGTGGT+GGG + chr4.3:74142220-74142239 MS.gene48984:CDS 45.0%
!! GATATTGTGAGGGGGTGTTT+AGG + chr4.3:74142180-74142199 MS.gene48984:CDS 45.0%
!! TTCACCGGCTTTCTTAGTAG+CGG - chr4.3:74141965-74141984 None:intergenic 45.0%
!!! ATTTTTGAGGCGGCGAATCA+TGG + chr4.3:74142126-74142145 MS.gene48984:CDS 45.0%
!!! CAAGCACTTTTGGAACGTAC+TGG + chr4.3:74141640-74141659 MS.gene48984:CDS 45.0%
AACTGCACATCCCCATTCCT+CGG - chr4.3:74142375-74142394 None:intergenic 50.0%
ACGGACACGTTTCTCCCAAA+CGG - chr4.3:74141455-74141474 None:intergenic 50.0%
AGCTGGTGTGAGGTATGCTA+TGG + chr4.3:74141759-74141778 MS.gene48984:CDS 50.0%
AGTTATGCGCAGAGAGAGCT+TGG + chr4.3:74142411-74142430 MS.gene48984:CDS 50.0%
ATCACTAACAGGAACAGGCG+AGG - chr4.3:74142292-74142311 None:intergenic 50.0%
ATGCATTTGGCTGCTCAAGC+TGG + chr4.3:74141742-74141761 MS.gene48984:CDS 50.0%
CTCCAAAGACAAAGCTTCGC+CGG - chr4.3:74141319-74141338 None:intergenic 50.0%
CTGTTTATGGTCCTTGGGGT+AGG + chr4.3:74142046-74142065 MS.gene48984:CDS 50.0%
CTTACTTCAACCGTGTACGG+TGG + chr4.3:74141284-74141303 MS.gene48984:CDS 50.0%
GAATCATGGTACCGTTGCGA+GGG + chr4.3:74142140-74142159 MS.gene48984:CDS 50.0%
GGATTCTCCGTTCTTGTGAC+CGG + chr4.3:74141502-74141521 MS.gene48984:CDS 50.0%
GTAGCGGCATACAAACTAGC+AGG - chr4.3:74141949-74141968 None:intergenic 50.0%
TTGAAAACCCTCAACTGCGC+CGG - chr4.3:74142258-74142277 None:intergenic 50.0%
! GCGTGCAAGACAAGCACTTT+TGG + chr4.3:74141630-74141649 MS.gene48984:CDS 50.0%
! TGGAAGTGGTGGGAAGAAGA+GGG + chr4.3:74142230-74142249 MS.gene48984:CDS 50.0%
!! GAGAAGAGTACTGGAAGTGG+TGG + chr4.3:74142219-74142238 MS.gene48984:CDS 50.0%
!! GCTGAGAAGAGTACTGGAAG+TGG + chr4.3:74142216-74142235 MS.gene48984:CDS 50.0%
AAACTGGGGTGGTCCCGTTT+GGG + chr4.3:74141438-74141457 MS.gene48984:CDS 55.0%
AGCAGCTCCGGTCACAAGAA+CGG - chr4.3:74141512-74141531 None:intergenic 55.0%
AGGAGTCCTTCAAACTGGGG+TGG + chr4.3:74141427-74141446 MS.gene48984:CDS 55.0%
CGAATCATGGTACCGTTGCG+AGG + chr4.3:74142139-74142158 MS.gene48984:CDS 55.0%
CGGACACGTTTCTCCCAAAC+GGG - chr4.3:74141454-74141473 None:intergenic 55.0%
GACGCCGGGAAAGTTCAAGA+TGG + chr4.3:74141252-74141271 MS.gene48984:CDS 55.0%
GGAGAGGCTGTTGAAGACCA+AGG + chr4.3:74142326-74142345 MS.gene48984:CDS 55.0%
TCCCTAAAACACCGTCTCCG+CGG - chr4.3:74141573-74141592 None:intergenic 55.0%
TCCTCAATGACCGCCGTGAA+GGG - chr4.3:74141411-74141430 None:intergenic 55.0%
TCCTTGGGGTAGGCCTGATA+TGG + chr4.3:74142056-74142075 MS.gene48984:CDS 55.0%
! CTCCGGCGAAGCTTTGTCTT+TGG + chr4.3:74141314-74141333 MS.gene48984:CDS 55.0%
! CTGGAAGTGGTGGGAAGAAG+AGG + chr4.3:74142229-74142248 MS.gene48984:CDS 55.0%
! GAAGTGGTGGGAAGAAGAGG+GGG + chr4.3:74142232-74142251 MS.gene48984:CDS 55.0%
! GGAAGTGGTGGGAAGAAGAG+GGG + chr4.3:74142231-74142250 MS.gene48984:CDS 55.0%
! TTGAACTTTCCCGGCGTCGA+CGG - chr4.3:74141250-74141269 None:intergenic 55.0%
!! GAGGGGGTGTTTAGGTGCTT+TGG + chr4.3:74142188-74142207 MS.gene48984:CDS 55.0%
!! TAAACCGGTGACGGATCTGC+AGG + chr4.3:74142437-74142456 MS.gene48984:CDS 55.0%
ACCCTTCACGGCGGTCATTG+AGG + chr4.3:74141407-74141426 MS.gene48984:CDS 60.0%
ACCGGACTCTAGCGGAGGAA+CGG - chr4.3:74141474-74141493 None:intergenic 60.0%
ACGGGACCACCCCAGTTTGA+AGG - chr4.3:74141436-74141455 None:intergenic 60.0%
CAAACTGGGGTGGTCCCGTT+TGG + chr4.3:74141437-74141456 MS.gene48984:CDS 60.0%
CGGAGAATCCGTTCCGTGAC+CGG - chr4.3:74141492-74141511 None:intergenic 60.0%
CGTCGGAACACATGTCTCCG+CGG + chr4.3:74141537-74141556 MS.gene48984:CDS 60.0%
CTCCTCAATGACCGCCGTGA+AGG - chr4.3:74141412-74141431 None:intergenic 60.0%
GAGGAGAAGTCGCCGGAGAA+CGG - chr4.3:74141378-74141397 None:intergenic 60.0%
GCCATATCAGGCCTACCCCA+AGG - chr4.3:74142060-74142079 None:intergenic 60.0%
GCTAGAGTCCGGTCACGGAA+CGG + chr4.3:74141481-74141500 MS.gene48984:CDS 60.0%
GTTCCGTGACCGGACTCTAG+CGG - chr4.3:74141482-74141501 None:intergenic 60.0%
GTTCTTGTGACCGGAGCTGC+TGG + chr4.3:74141511-74141530 MS.gene48984:CDS 60.0%
TAACAGTGCTCCGTCGACGC+CGG + chr4.3:74141237-74141256 MS.gene48984:CDS 60.0%
TCCGACGAAACCAGCAGCTC+CGG - chr4.3:74141524-74141543 None:intergenic 60.0%
TCCGGCGACTTCTCCTCTTC+CGG + chr4.3:74141381-74141400 MS.gene48984:CDS 60.0%
TCCGTTCCTCCGCTAGAGTC+CGG + chr4.3:74141470-74141489 MS.gene48984:CDS 60.0%
TGGCTGCTCAAGCTGGTGTG+AGG + chr4.3:74141749-74141768 MS.gene48984:CDS 60.0%
TGTACGGTGGCACGCTTCTC+CGG + chr4.3:74141297-74141316 MS.gene48984:CDS 60.0%
! GAGAAGCGTGCCACCGTACA+CGG - chr4.3:74141297-74141316 None:intergenic 60.0%
! GCCGCGGAGACGGTGTTTTA+GGG + chr4.3:74141569-74141588 MS.gene48984:CDS 60.0%
!! ACCGGAGCTGCTGGTTTCGT+CGG + chr4.3:74141520-74141539 MS.gene48984:CDS 60.0%
AACAGTGCTCCGTCGACGCC+GGG + chr4.3:74141238-74141257 MS.gene48984:CDS 65.0%
CCAGCCTGCAGATCCGTCAC+CGG - chr4.3:74142444-74142463 None:intergenic 65.0%
CCTCCGCTAGAGTCCGGTCA+CGG + chr4.3:74141476-74141495 MS.gene48984:CDS 65.0%
CTCTTCCGGCGGACCCTTCA+CGG + chr4.3:74141395-74141414 MS.gene48984:CDS 65.0%
GCCGGAAGAGGAGAAGTCGC+CGG - chr4.3:74141385-74141404 None:intergenic 65.0%
GCGCTTAAACGCCGCGGAGA+CGG + chr4.3:74141559-74141578 MS.gene48984:CDS 65.0%
GGCGACTTCTCCTCTTCCGG+CGG + chr4.3:74141384-74141403 MS.gene48984:CDS 65.0%
TGGTGGGAAGAAGAGGGGGC+CGG + chr4.3:74142236-74142255 MS.gene48984:CDS 65.0%
! CGCCGCGGAGACGGTGTTTT+AGG + chr4.3:74141568-74141587 MS.gene48984:CDS 65.0%
CCGTGACCGGACTCTAGCGG+AGG - chr4.3:74141479-74141498 None:intergenic 70.0%
CGTGAAGGGTCCGCCGGAAG+AGG - chr4.3:74141397-74141416 None:intergenic 70.0%
TCCGCGGCGCTTAAACGCCG+CGG + chr4.3:74141553-74141572 MS.gene48984:CDS 70.0%
TTCCGGCGGACCCTTCACGG+CGG + chr4.3:74141398-74141417 MS.gene48984:CDS 70.0%
! TCCGCGGCGTTTAAGCGCCG+CGG - chr4.3:74141557-74141576 None:intergenic 70.0%
!! CCGGTGACGGATCTGCAGGC+TGG + chr4.3:74142441-74142460 MS.gene48984:CDS 70.0%
AGAGGGGGCCGGCGCAGTTG+AGG + chr4.3:74142247-74142266 MS.gene48984:CDS 75.0%
GACCGCCGTGAAGGGTCCGC+CGG - chr4.3:74141403-74141422 None:intergenic 75.0%
GAGGGGGCCGGCGCAGTTGA+GGG + chr4.3:74142248-74142267 MS.gene48984:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 74141205 74142527 74141205 ID=MS.gene48984
chr4.3 mRNA 74141205 74142527 74141205 ID=MS.gene48984.t1;Parent=MS.gene48984
chr4.3 exon 74141205 74142527 74141205 ID=MS.gene48984.t1.exon1;Parent=MS.gene48984.t1
chr4.3 CDS 74141205 74142527 74141205 ID=cds.MS.gene48984.t1;Parent=MS.gene48984.t1
Gene Sequence

>MS.gene48984

ATGTCTCAGCTGAAGCAAATGTCGCACGCTGATAACAGTGCTCCGTCGACGCCGGGAAAGTTCAAGATGGAGAAAGCTTCTTACTTCAACCGTGTACGGTGGCACGCTTCTCCGGCGAAGCTTTGTCTTTGGAGTTTTGTTTTCTCAGCTGCGATCTTGATCTTCTTTTTCCGTTCTCCGGCGACTTCTCCTCTTCCGGCGGACCCTTCACGGCGGTCATTGAGGAGTCCTTCAAACTGGGGTGGTCCCGTTTGGGAGAAACGTGTCCGTTCCTCCGCTAGAGTCCGGTCACGGAACGGATTCTCCGTTCTTGTGACCGGAGCTGCTGGTTTCGTCGGAACACATGTCTCCGCGGCGCTTAAACGCCGCGGAGACGGTGTTTTAGGGATTGATAATTTCAATGACTATTATGATCCTTCTTTGAAGCGTGCAAGACAAGCACTTTTGGAACGTACTGGTGTTTTCATTGTTGAAGGTGATATCAATGATGCTGCTTTGTTAAGGAAGCTTTTTGAGGTTGTTCCTTTTACTCATGTCATGCATTTGGCTGCTCAAGCTGGTGTGAGGTATGCTATGGAGAATCCTGGTTCTTATGTTCATAGTAACATTGCTGGTTTTGTTAATTTGCTTGAGGTTTGTAAAAGTGTTAATCCTCAACCTTCAATTGTTTGGGCTAGTTCTAGTTCTGTTTATGGATTGAATACTAAGGTTCCTTTTTCTGAAAGGGATAGAACTGATCAACCTGCTAGTTTGTATGCCGCTACTAAGAAAGCCGGTGAAGAAATCGCGCATACTTATAATCATATCTATGGTCTTTCTTTAACTGGTTTGAGGTTTTTCACTGTTTATGGTCCTTGGGGTAGGCCTGATATGGCTTATTTCTTTTTCACTAGAGATATTTTGAAGGGGAAGACTATTCCTATTTTTGAGGCGGCGAATCATGGTACCGTTGCGAGGGATTTTACTTATATTGATGATATTGTGAGGGGGTGTTTAGGTGCTTTGGATACTGCTGAGAAGAGTACTGGAAGTGGTGGGAAGAAGAGGGGGCCGGCGCAGTTGAGGGTTTTCAATTTGGGGAATACCTCGCCTGTTCCTGTTAGTGATCTTGTGGGTATTTTGGAGAGGCTGTTGAAGACCAAGGCAAAGAGGAATATTATGAAGTTGCCGAGGAATGGGGATGTGCAGTTTACTCATGCGAATATTAGTTATGCGCAGAGAGAGCTTGGTTATAAACCGGTGACGGATCTGCAGGCTGGTTTGAAGAAATTTGTGAGGTGGTATCTGAATTACTATTCTTCTGGGAAGAAAGCTGTTGAGTGA
Protein sequence

>MS.gene48984.t1

MSQLKQMSHADNSAPSTPGKFKMEKASYFNRVRWHASPAKLCLWSFVFSAAILIFFFRSPATSPLPADPSRRSLRSPSNWGGPVWEKRVRSSARVRSRNGFSVLVTGAAGFVGTHVSAALKRRGDGVLGIDNFNDYYDPSLKRARQALLERTGVFIVEGDINDAALLRKLFEVVPFTHVMHLAAQAGVRYAMENPGSYVHSNIAGFVNLLEVCKSVNPQPSIVWASSSSVYGLNTKVPFSERDRTDQPASLYAATKKAGEEIAHTYNHIYGLSLTGLRFFTVYGPWGRPDMAYFFFTRDILKGKTIPIFEAANHGTVARDFTYIDDIVRGCLGALDTAEKSTGSGGKKRGPAQLRVFNLGNTSPVPVSDLVGILERLLKTKAKRNIMKLPRNGDVQFTHANISYAQRELGYKPVTDLQAGLKKFVRWYLNYYSSGKKAVE