AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99045

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99045.t1 MTR_4g024600 99.773 440 1 0 1 440 1 440 0.0 907
MS.gene99045.t1 MTR_0354s0040 82.629 426 66 3 15 440 4 421 0.0 728
MS.gene99045.t1 MTR_4g102270 69.431 422 125 3 11 432 7 424 0.0 603
MS.gene99045.t1 MTR_4g129820 67.431 436 132 4 8 440 6 434 0.0 593
MS.gene99045.t1 MTR_3g084090 66.047 430 134 3 13 432 3 430 0.0 585
MS.gene99045.t1 MTR_5g011940 76.294 367 85 1 69 433 56 422 0.0 581
MS.gene99045.t1 MTR_0034s0060 27.545 334 213 8 102 428 138 449 9.11e-22 98.6
MS.gene99045.t1 MTR_8g096880 28.614 332 199 12 103 412 8 323 1.45e-21 95.9
MS.gene99045.t1 MTR_8g096880 29.042 334 195 13 103 412 8 323 2.08e-21 94.7
MS.gene99045.t1 MTR_2g073240 26.822 343 208 8 102 430 20 333 2.62e-20 94.0
MS.gene99045.t1 MTR_2g032100 26.087 345 219 12 103 427 73 401 2.65e-20 92.8
MS.gene99045.t1 MTR_5g009170 26.039 361 230 12 91 433 38 379 3.38e-20 92.4
MS.gene99045.t1 MTR_8g099535 27.576 330 205 12 102 412 5 319 7.92e-20 90.9
MS.gene99045.t1 MTR_1g009600 25.519 337 220 10 102 430 6 319 3.17e-19 90.9
MS.gene99045.t1 MTR_4g120030 25.397 441 251 17 8 425 20 405 6.54e-19 89.0
MS.gene99045.t1 MTR_5g009160 26.923 338 203 11 99 412 2 319 1.07e-18 87.4
MS.gene99045.t1 MTR_1g088480 25.816 337 202 12 98 425 115 412 1.20e-18 88.2
MS.gene99045.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene99045.t1 MTR_8g089180 25.143 350 226 11 103 432 73 406 1.37e-17 84.7
MS.gene99045.t1 MTR_4g063600 24.471 331 212 11 98 421 29 328 2.91e-17 83.2
MS.gene99045.t1 MTR_8g096880 28.041 296 176 11 103 376 8 288 8.39e-17 81.3
MS.gene99045.t1 MTR_4g063600 24.773 331 208 12 98 421 29 325 2.90e-16 80.1
MS.gene99045.t1 MTR_7g115080 25.000 336 210 13 103 427 31 335 2.48e-15 77.8
MS.gene99045.t1 MTR_7g115080 25.000 336 210 13 103 427 65 369 2.64e-15 77.8
MS.gene99045.t1 MTR_7g115080 25.000 336 210 13 103 427 44 348 2.70e-15 77.8
MS.gene99045.t1 MTR_1g080950 23.810 399 247 16 41 427 18 371 4.46e-15 77.0
MS.gene99045.t1 MTR_2g032100 29.630 189 127 5 103 288 73 258 6.25e-15 75.5
MS.gene99045.t1 MTR_1g080950 24.189 339 214 12 101 427 28 335 8.07e-15 76.3
MS.gene99045.t1 MTR_2g096660 24.324 333 210 12 98 421 35 334 1.67e-14 75.1
MS.gene99045.t1 MTR_4g101940 86.667 30 4 0 173 202 42 71 2.71e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99045.t1 AT4G00110 86.667 435 51 4 4 438 1 428 0.0 770
MS.gene99045.t1 AT1G02000 84.828 435 59 5 7 439 1 430 0.0 751
MS.gene99045.t1 AT2G45310 78.851 435 88 3 7 438 1 434 0.0 693
MS.gene99045.t1 AT4G12250 73.059 438 105 4 7 437 1 432 0.0 621
MS.gene99045.t1 AT3G23820 77.557 352 79 0 81 432 92 443 0.0 582
MS.gene99045.t1 AT4G30440 67.907 430 126 4 11 438 6 425 0.0 564
MS.gene99045.t1 AT4G23920 27.350 351 215 12 102 430 4 336 4.19e-23 100
MS.gene99045.t1 AT1G12780 28.657 335 197 13 102 412 8 324 1.10e-22 99.0
MS.gene99045.t1 AT1G63180 28.443 334 199 12 102 412 8 324 5.08e-21 94.4
MS.gene99045.t1 AT1G78570 26.647 334 216 8 102 428 8 319 8.81e-21 95.5
MS.gene99045.t1 AT3G14790 26.647 334 216 8 102 428 11 322 2.27e-20 94.4
MS.gene99045.t1 AT3G14790 26.647 334 216 8 102 428 8 319 2.54e-20 94.4
MS.gene99045.t1 AT4G10960 26.891 357 209 13 102 430 5 337 7.75e-20 90.9
MS.gene99045.t1 AT1G30620 25.507 345 221 11 103 427 72 400 4.10e-19 89.4
MS.gene99045.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene99045.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene99045.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene99045.t1 AT1G30620 25.507 345 221 11 103 427 73 401 4.49e-19 89.4
MS.gene99045.t1 AT1G53500 26.393 341 208 8 102 428 10 321 9.08e-19 89.4
MS.gene99045.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene99045.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene99045.t1 AT5G44480 24.709 344 225 10 103 427 73 401 1.79e-18 87.4
MS.gene99045.t1 AT5G44480 24.709 344 225 10 103 427 97 425 2.18e-18 87.4
MS.gene99045.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene99045.t1 AT3G62830 25.507 345 200 12 98 426 116 419 8.22e-18 85.9
MS.gene99045.t1 AT3G53520 25.294 340 215 10 98 430 117 424 1.40e-17 85.1
MS.gene99045.t1 AT1G64440 25.281 356 220 12 102 434 4 336 1.42e-17 84.0
MS.gene99045.t1 AT2G47650 24.122 427 252 16 32 426 35 421 3.98e-17 83.6
MS.gene99045.t1 AT4G20460 25.212 353 227 12 96 427 64 400 4.53e-17 83.2
MS.gene99045.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene99045.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene99045.t1 AT2G28760 25.225 333 207 13 98 421 28 327 6.37e-17 82.0
MS.gene99045.t1 AT2G28760 25.225 333 207 13 98 421 59 358 8.34e-17 82.0
MS.gene99045.t1 AT1G63180 28.188 298 175 11 102 376 8 289 9.29e-17 81.3
MS.gene99045.t1 AT3G53520 25.000 340 214 11 98 430 117 422 1.72e-15 78.6
MS.gene99045.t1 AT2G47650 24.249 433 250 18 32 426 35 427 3.98e-15 77.4
MS.gene99045.t1 AT5G59290 24.085 328 207 12 103 421 47 341 7.57e-15 76.3
MS.gene99045.t1 AT5G59290 24.085 328 207 12 103 421 32 326 8.39e-15 75.9
MS.gene99045.t1 AT5G59290 24.085 328 207 12 103 421 3 297 9.21e-15 75.5
MS.gene99045.t1 AT5G59290 24.085 328 207 12 103 421 25 319 1.03e-14 75.5
MS.gene99045.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene99045.t1 AT3G46440 23.353 334 214 12 97 421 25 325 1.25e-14 75.1
MS.gene99045.t1 AT3G53520 27.083 240 153 6 98 331 117 340 2.60e-14 74.3
MS.gene99045.t1 AT3G53520 24.011 354 225 12 98 430 117 447 3.55e-14 74.7
MS.gene99045.t1 AT1G30620 27.128 188 133 3 103 288 73 258 1.37e-13 71.6

Find 116 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAGTTCTAGTTCTGTTTA+TGG 0.169613 4.1:+74204605 MS.gene99045:CDS
GTTCTATCCCTTTCAGAAAA+AGG 0.194999 4.1:-74204644 None:intergenic
ATCTATGGTCTTTCTTTAAC+TGG 0.228130 4.1:+74204737 MS.gene99045:CDS
ATCCTCAACCTTCAATTGTT+TGG 0.242672 4.1:+74204582 MS.gene99045:CDS
CTCCGGCGAAGCTTTGTCTT+TGG 0.247408 4.1:+74204042 MS.gene99045:CDS
TATCTGAATTACTATTCTTC+TGG 0.256882 4.1:+74205214 MS.gene99045:CDS
TAGTGATCTTGTGGGTATTT+TGG 0.296077 4.1:+74205033 MS.gene99045:CDS
TTCTCCATCTTGAACTTTCC+CGG 0.301644 4.1:-74203984 None:intergenic
TCCTCAACCTTCAATTGTTT+GGG 0.302294 4.1:+74204583 MS.gene99045:CDS
TTTCACTGTTTATGGTCCTT+GGG 0.318056 4.1:+74204769 MS.gene99045:CDS
AAATGCATGACATGAGTAAA+AGG 0.326266 4.1:-74204455 None:intergenic
TCCTTGGGGTAGGCCTGATA+TGG 0.329537 4.1:+74204784 MS.gene99045:CDS
CAAACTGGGGTGGTCCCGTT+TGG 0.334864 4.1:+74204165 MS.gene99045:CDS
GCGTGCAAGACAAGCACTTT+TGG 0.348677 4.1:+74204358 MS.gene99045:CDS
GTCTTTCTTTAACTGGTTTG+AGG 0.353381 4.1:+74204744 MS.gene99045:CDS
TATGTTCATAGTAACATTGC+TGG 0.365279 4.1:+74204524 MS.gene99045:CDS
CATTGAGGAGTCCTTCAAAC+TGG 0.372195 4.1:+74204150 MS.gene99045:CDS
CATACTTATAATCATATCTA+TGG 0.374841 4.1:+74204722 MS.gene99045:CDS
CTCTTCCGGCGGACCCTTCA+CGG 0.384471 4.1:+74204123 MS.gene99045:CDS
AAACTGGGGTGGTCCCGTTT+GGG 0.395396 4.1:+74204166 MS.gene99045:CDS
TTATGAAGTTGCCGAGGAAT+GGG 0.400306 4.1:+74205089 MS.gene99045:CDS
ATCTGAATTACTATTCTTCT+GGG 0.411188 4.1:+74205215 MS.gene99045:CDS
CTGTTTATGGTCCTTGGGGT+AGG 0.421551 4.1:+74204774 MS.gene99045:CDS
CTGGAAGTGGTGGGAAGAAG+AGG 0.431142 4.1:+74204957 MS.gene99045:CDS
TCAATGATGCTGCTTTGTTA+AGG 0.431669 4.1:+74204414 MS.gene99045:CDS
GTTCTTGTGACCGGAGCTGC+TGG 0.437389 4.1:+74204239 MS.gene99045:CDS
TGTCTTGCACGCTTCAAAGA+AGG 0.438967 4.1:-74204347 None:intergenic
TCCGGCGACTTCTCCTCTTC+CGG 0.441112 4.1:+74204109 MS.gene99045:CDS
GGAGAGGCTGTTGAAGACTA+AGG 0.452325 4.1:+74205054 MS.gene99045:CDS
TGTACGGTGGCACGCTTCTC+CGG 0.452864 4.1:+74204025 MS.gene99045:CDS
GAACTAGCCCAAACAATTGA+AGG 0.455646 4.1:-74204590 None:intergenic
ATACCCACAAGATCACTAAC+AGG 0.465851 4.1:-74205028 None:intergenic
AACAGTGCTCCGTCGACGCC+GGG 0.468408 4.1:+74203966 MS.gene99045:CDS
ATTATGAAGTTGCCGAGGAA+TGG 0.468878 4.1:+74205088 MS.gene99045:CDS
ATGCATTTGGCTGCTCAAGC+TGG 0.474694 4.1:+74204470 MS.gene99045:CDS
ACAAGATCACTAACAGGAAC+AGG 0.475033 4.1:-74205022 None:intergenic
CGTGAAGGGTCCGCCGGAAG+AGG 0.475778 4.1:-74204122 None:intergenic
TCCGACGAAACCAGCAGCTC+CGG 0.477531 4.1:-74204249 None:intergenic
AGTTATGCACAGAGAGAGCT+TGG 0.481744 4.1:+74205139 MS.gene99045:CDS
GTTCCTGTTAGTGATCTTGT+GGG 0.482574 4.1:+74205025 MS.gene99045:CDS
TGTTTATGGACTGAATACTA+AGG 0.487187 4.1:+74204619 MS.gene99045:CDS
CGGAGAATCCGTTCCGTGAC+CGG 0.489325 4.1:-74204217 None:intergenic
TCCGTTCCTCCGCTAGAGTC+CGG 0.497044 4.1:+74204198 MS.gene99045:CDS
CTCCAAAGACAAAGCTTCGC+CGG 0.498173 4.1:-74204044 None:intergenic
CGGACACGTTTCTCCCAAAC+GGG 0.500537 4.1:-74204179 None:intergenic
GATACTGCTGAGAAGAGTAC+TGG 0.501421 4.1:+74204938 MS.gene99045:CDS
CTTCTTACTTCAACCGTGTA+CGG 0.502910 4.1:+74204009 MS.gene99045:CDS
ACGGGACCACCCCAGTTTGA+AGG 0.504384 4.1:-74204161 None:intergenic
GGATTCTCCGTTCTTGTGAC+CGG 0.510300 4.1:+74204230 MS.gene99045:CDS
GAGAGAGCTTGGTTATAAAC+CGG 0.513102 4.1:+74205150 MS.gene99045:CDS
TAAACCGGTGACGGATCTGC+AGG 0.513367 4.1:+74205165 MS.gene99045:CDS
GACCGCCGTGAAGGGTCCGC+CGG 0.515234 4.1:-74204128 None:intergenic
GCGCTTAAACGCCGCGGAGA+CGG 0.515369 4.1:+74204287 MS.gene99045:CDS
TGTTCCTGTTAGTGATCTTG+TGG 0.519953 4.1:+74205024 MS.gene99045:CDS
ACGGACACGTTTCTCCCAAA+CGG 0.521018 4.1:-74204180 None:intergenic
GTCTTGCACGCTTCAAAGAA+GGG 0.524206 4.1:-74204346 None:intergenic
AGTATGCGCGATTTCTTCAC+CGG 0.527758 4.1:-74204705 None:intergenic
CTCCTCAATGACCGCCGTGA+AGG 0.532413 4.1:-74204137 None:intergenic
GCCCAAACAATTGAAGGTTG+AGG 0.535698 4.1:-74204584 None:intergenic
ACCGGAGCTGCTGGTTTCGT+CGG 0.536422 4.1:+74204248 MS.gene99045:CDS
TGGAAGTGGTGGGAAGAAGA+GGG 0.543352 4.1:+74204958 MS.gene99045:CDS
TTCACTGTTTATGGTCCTTG+GGG 0.546844 4.1:+74204770 MS.gene99045:CDS
ACCCTTCACGGCGGTCATTG+AGG 0.548511 4.1:+74204135 MS.gene99045:CDS
ATTGAGGAGTCCTTCAAACT+GGG 0.549385 4.1:+74204151 MS.gene99045:CDS
TTATATTGATGATATTGTGA+GGG 0.550359 4.1:+74204898 MS.gene99045:CDS
GAGGAGAAGTCGCCGGAGAA+CGG 0.554120 4.1:-74204103 None:intergenic
AACTGCACATCCCCATTCCT+CGG 0.554769 4.1:-74205100 None:intergenic
TCCTCAATGACCGCCGTGAA+GGG 0.559853 4.1:-74204136 None:intergenic
TAACAGTGCTCCGTCGACGC+CGG 0.562127 4.1:+74203965 MS.gene99045:CDS
GTAGCGGCATACAAACTAGC+AGG 0.564565 4.1:-74204674 None:intergenic
TTGAACTTTCCCGGCGTCGA+CGG 0.565515 4.1:-74203975 None:intergenic
TTCACCGGCTTTCTTAGTAG+CGG 0.567698 4.1:-74204690 None:intergenic
GAGAAGCGTGCCACCGTACA+CGG 0.567964 4.1:-74204022 None:intergenic
AGCAGCTCCGGTCACAAGAA+CGG 0.569637 4.1:-74204237 None:intergenic
TTGAAAACCCTCAACTGCGC+CGG 0.571733 4.1:-74204983 None:intergenic
CCGGTGACGGATCTGCAGGC+TGG 0.573224 4.1:+74205169 MS.gene99045:CDS
ACCGGACTCTAGCGGAGGAA+CGG 0.574251 4.1:-74204199 None:intergenic
CTTATATTGATGATATTGTG+AGG 0.578974 4.1:+74204897 MS.gene99045:CDS
GAAGTGGTGGGAAGAAGAGG+GGG 0.582983 4.1:+74204960 MS.gene99045:CDS
CGAATCATGGTACCGTTGCG+AGG 0.585042 4.1:+74204867 MS.gene99045:CDS
AGCTGGTGTGAGGTATGCTA+TGG 0.586552 4.1:+74204487 MS.gene99045:CDS
AGGTATGCTATGGAGAATCC+TGG 0.586560 4.1:+74204497 MS.gene99045:CDS
CCAGCCTGCAGATCCGTCAC+CGG 0.597830 4.1:-74205169 None:intergenic
GGCGACTTCTCCTCTTCCGG+CGG 0.605382 4.1:+74204112 MS.gene99045:CDS
GGAAGTGGTGGGAAGAAGAG+GGG 0.607007 4.1:+74204959 MS.gene99045:CDS
CCTCCGCTAGAGTCCGGTCA+CGG 0.608900 4.1:+74204204 MS.gene99045:CDS
TATGAAGTTGCCGAGGAATG+GGG 0.609964 4.1:+74205090 MS.gene99045:CDS
GCTAGAGTCCGGTCACGGAA+CGG 0.610673 4.1:+74204209 MS.gene99045:CDS
GAATCATGGTACCGTTGCGA+GGG 0.611200 4.1:+74204868 MS.gene99045:CDS
GCCGGAAGAGGAGAAGTCGC+CGG 0.613076 4.1:-74204110 None:intergenic
TATGCCGCTACTAAGAAAGC+CGG 0.617216 4.1:+74204686 MS.gene99045:CDS
GCTTGGTTATAAACCGGTGA+CGG 0.619019 4.1:+74205156 MS.gene99045:CDS
GAGAAGAGTACTGGAAGTGG+TGG 0.623154 4.1:+74204947 MS.gene99045:CDS
GTTTGAAGAAATTTGTGAGG+TGG 0.624134 4.1:+74205191 MS.gene99045:CDS
TGGCTGCTCAAGCTGGTGTG+AGG 0.625330 4.1:+74204477 MS.gene99045:CDS
GCCATATCAGGCCTACCCCA+AGG 0.630669 4.1:-74204785 None:intergenic
GCTGAGAAGAGTACTGGAAG+TGG 0.633054 4.1:+74204944 MS.gene99045:CDS
TCCGCGGCGTTTAAGCGCTG+CGG 0.633403 4.1:-74204282 None:intergenic
GACGCCGGGAAAGTTCAAGA+TGG 0.634115 4.1:+74203980 MS.gene99045:CDS
CTGGTTTGAAGAAATTTGTG+AGG 0.647230 4.1:+74205188 MS.gene99045:CDS
AGGAGTCCTTCAAACTGGGG+TGG 0.647390 4.1:+74204155 MS.gene99045:CDS
ATAAGTAAAATCCCTCGCAA+CGG 0.649166 4.1:-74204879 None:intergenic
TCCCTAAAACACCGTCTCCG+CGG 0.649971 4.1:-74204298 None:intergenic
AGAAGAGTACTGGAAGTGGT+GGG 0.649979 4.1:+74204948 MS.gene99045:CDS
GTTCCGTGACCGGACTCTAG+CGG 0.650330 4.1:-74204207 None:intergenic
GGAATATTATGAAGTTGCCG+AGG 0.656687 4.1:+74205083 MS.gene99045:CDS
CTTACTTCAACCGTGTACGG+TGG 0.659507 4.1:+74204012 MS.gene99045:CDS
CCGTGACCGGACTCTAGCGG+AGG 0.659987 4.1:-74204204 None:intergenic
TATATTGATGATATTGTGAG+GGG 0.666488 4.1:+74204899 MS.gene99045:CDS
ATCACTAACAGGAACAGGCG+AGG 0.666779 4.1:-74205017 None:intergenic
TCCGCAGCGCTTAAACGCCG+CGG 0.666946 4.1:+74204281 MS.gene99045:CDS
TGTTGAAGACTAAGGCGAAG+AGG 0.671437 4.1:+74205062 MS.gene99045:CDS
TTACTATGAACATAAGAACC+AGG 0.683714 4.1:-74204515 None:intergenic
TTGAGGAGTCCTTCAAACTG+GGG 0.690015 4.1:+74204152 MS.gene99045:CDS
TTCCGGCGGACCCTTCACGG+CGG 0.735183 4.1:+74204126 MS.gene99045:CDS
ATATTGATGATATTGTGAGG+GGG 0.741076 4.1:+74204900 MS.gene99045:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATACTTATAATCATATCTA+TGG + chr4.1:74204722-74204741 MS.gene99045:CDS 20.0%
!!! TTATATTGATGATATTGTGA+GGG + chr4.1:74204898-74204917 MS.gene99045:CDS 20.0%
! AAAAAGAAATAAGCCATATC+AGG - chr4.1:74204800-74204819 None:intergenic 25.0%
! ATCTGAATTACTATTCTTCT+GGG + chr4.1:74205215-74205234 MS.gene99045:CDS 25.0%
! TATCTGAATTACTATTCTTC+TGG + chr4.1:74205214-74205233 MS.gene99045:CDS 25.0%
!! CTTATATTGATGATATTGTG+AGG + chr4.1:74204897-74204916 MS.gene99045:CDS 25.0%
!! TATATTGATGATATTGTGAG+GGG + chr4.1:74204899-74204918 MS.gene99045:CDS 25.0%
!!! TTCACTAGAGATATTTTGAA+GGG + chr4.1:74204818-74204837 MS.gene99045:CDS 25.0%
!!! TTTCACTAGAGATATTTTGA+AGG + chr4.1:74204817-74204836 MS.gene99045:CDS 25.0%
AAATGCATGACATGAGTAAA+AGG - chr4.1:74204458-74204477 None:intergenic 30.0%
ATCTATGGTCTTTCTTTAAC+TGG + chr4.1:74204737-74204756 MS.gene99045:CDS 30.0%
TATGTTCATAGTAACATTGC+TGG + chr4.1:74204524-74204543 MS.gene99045:CDS 30.0%
TTACTATGAACATAAGAACC+AGG - chr4.1:74204518-74204537 None:intergenic 30.0%
! ATATTGATGATATTGTGAGG+GGG + chr4.1:74204900-74204919 MS.gene99045:CDS 30.0%
! TGTTTATGGACTGAATACTA+AGG + chr4.1:74204619-74204638 MS.gene99045:CDS 30.0%
! TTGAGGTTTTTCACTGTTTA+TGG + chr4.1:74204761-74204780 MS.gene99045:CDS 30.0%
! TTTGTTAAGGAAGCTTTTTG+AGG + chr4.1:74204427-74204446 MS.gene99045:CDS 30.0%
! TTTTACTCATGTCATGCATT+TGG + chr4.1:74204457-74204476 MS.gene99045:CDS 30.0%
!! TAAGGTTCCTTTTTCTGAAA+GGG + chr4.1:74204637-74204656 MS.gene99045:CDS 30.0%
!! TCACTAGAGATATTTTGAAG+GGG + chr4.1:74204819-74204838 MS.gene99045:CDS 30.0%
!!! GAAGACTATTCCTATTTTTG+AGG + chr4.1:74204841-74204860 MS.gene99045:CDS 30.0%
!!! TGGTTTTGTTAATTTGCTTG+AGG + chr4.1:74204544-74204563 MS.gene99045:CDS 30.0%
ATAAGTAAAATCCCTCGCAA+CGG - chr4.1:74204882-74204901 None:intergenic 35.0%
ATCCTCAACCTTCAATTGTT+TGG + chr4.1:74204582-74204601 MS.gene99045:CDS 35.0%
GTTCTATCCCTTTCAGAAAA+AGG - chr4.1:74204647-74204666 None:intergenic 35.0%
GTTTGAAGAAATTTGTGAGG+TGG + chr4.1:74205191-74205210 MS.gene99045:CDS 35.0%
TCCTCAACCTTCAATTGTTT+GGG + chr4.1:74204583-74204602 MS.gene99045:CDS 35.0%
TTTCACTGTTTATGGTCCTT+GGG + chr4.1:74204769-74204788 MS.gene99045:CDS 35.0%
! ACTGGTGTTTTCATTGTTGA+AGG + chr4.1:74204386-74204405 MS.gene99045:CDS 35.0%
! AGTTGAGGGTTTTCAATTTG+GGG + chr4.1:74204990-74205009 MS.gene99045:CDS 35.0%
! CAGTTGAGGGTTTTCAATTT+GGG + chr4.1:74204989-74205008 MS.gene99045:CDS 35.0%
! CTAAGGTTCCTTTTTCTGAA+AGG + chr4.1:74204636-74204655 MS.gene99045:CDS 35.0%
! CTGGTTTGAAGAAATTTGTG+AGG + chr4.1:74205188-74205207 MS.gene99045:CDS 35.0%
! TAGTGATCTTGTGGGTATTT+TGG + chr4.1:74205033-74205052 MS.gene99045:CDS 35.0%
! TTTTCACTGTTTATGGTCCT+TGG + chr4.1:74204768-74204787 MS.gene99045:CDS 35.0%
!! GCTAGTTCTAGTTCTGTTTA+TGG + chr4.1:74204605-74204624 MS.gene99045:CDS 35.0%
!! GTCTTTCTTTAACTGGTTTG+AGG + chr4.1:74204744-74204763 MS.gene99045:CDS 35.0%
!! TCAATGATGCTGCTTTGTTA+AGG + chr4.1:74204414-74204433 MS.gene99045:CDS 35.0%
!!! GACTATTCCTATTTTTGAGG+CGG + chr4.1:74204844-74204863 MS.gene99045:CDS 35.0%
ACAAGATCACTAACAGGAAC+AGG - chr4.1:74205025-74205044 None:intergenic 40.0%
ATACCCACAAGATCACTAAC+AGG - chr4.1:74205031-74205050 None:intergenic 40.0%
ATTATGAAGTTGCCGAGGAA+TGG + chr4.1:74205088-74205107 MS.gene99045:CDS 40.0%
ATTGAGGAGTCCTTCAAACT+GGG + chr4.1:74204151-74204170 MS.gene99045:CDS 40.0%
CTTCTTACTTCAACCGTGTA+CGG + chr4.1:74204009-74204028 MS.gene99045:CDS 40.0%
GAACTAGCCCAAACAATTGA+AGG - chr4.1:74204593-74204612 None:intergenic 40.0%
GAGAGAGCTTGGTTATAAAC+CGG + chr4.1:74205150-74205169 MS.gene99045:CDS 40.0%
GGAATATTATGAAGTTGCCG+AGG + chr4.1:74205083-74205102 MS.gene99045:CDS 40.0%
GTTCCTGTTAGTGATCTTGT+GGG + chr4.1:74205025-74205044 MS.gene99045:CDS 40.0%
TGTTCCTGTTAGTGATCTTG+TGG + chr4.1:74205024-74205043 MS.gene99045:CDS 40.0%
TTATGAAGTTGCCGAGGAAT+GGG + chr4.1:74205089-74205108 MS.gene99045:CDS 40.0%
TTCACTGTTTATGGTCCTTG+GGG + chr4.1:74204770-74204789 MS.gene99045:CDS 40.0%
TTCTCCATCTTGAACTTTCC+CGG - chr4.1:74203987-74204006 None:intergenic 40.0%
! GATCTTCTTTTTCCGTTCTC+CGG + chr4.1:74204091-74204110 MS.gene99045:CDS 40.0%
! GCAGTTGAGGGTTTTCAATT+TGG + chr4.1:74204988-74205007 MS.gene99045:CDS 40.0%
!! ATCTTGTGGGTATTTTGGAG+AGG + chr4.1:74205038-74205057 MS.gene99045:CDS 40.0%
AGGTATGCTATGGAGAATCC+TGG + chr4.1:74204497-74204516 MS.gene99045:CDS 45.0%
AGTATGCGCGATTTCTTCAC+CGG - chr4.1:74204708-74204727 None:intergenic 45.0%
AGTTATGCACAGAGAGAGCT+TGG + chr4.1:74205139-74205158 MS.gene99045:CDS 45.0%
CATTGAGGAGTCCTTCAAAC+TGG + chr4.1:74204150-74204169 MS.gene99045:CDS 45.0%
GCCCAAACAATTGAAGGTTG+AGG - chr4.1:74204587-74204606 None:intergenic 45.0%
GCTTGGTTATAAACCGGTGA+CGG + chr4.1:74205156-74205175 MS.gene99045:CDS 45.0%
GTCTTGCACGCTTCAAAGAA+GGG - chr4.1:74204349-74204368 None:intergenic 45.0%
TATGAAGTTGCCGAGGAATG+GGG + chr4.1:74205090-74205109 MS.gene99045:CDS 45.0%
TATGCCGCTACTAAGAAAGC+CGG + chr4.1:74204686-74204705 MS.gene99045:CDS 45.0%
TGATTCGCCGCCTCAAAAAT+AGG - chr4.1:74204854-74204873 None:intergenic 45.0%
TGTCTTGCACGCTTCAAAGA+AGG - chr4.1:74204350-74204369 None:intergenic 45.0%
TGTTGAAGACTAAGGCGAAG+AGG + chr4.1:74205062-74205081 MS.gene99045:CDS 45.0%
TTGAGGAGTCCTTCAAACTG+GGG + chr4.1:74204152-74204171 MS.gene99045:CDS 45.0%
! GATACTGCTGAGAAGAGTAC+TGG + chr4.1:74204938-74204957 MS.gene99045:CDS 45.0%
!! AGAAGAGTACTGGAAGTGGT+GGG + chr4.1:74204948-74204967 MS.gene99045:CDS 45.0%
!! GATATTGTGAGGGGGTGTTT+AGG + chr4.1:74204908-74204927 MS.gene99045:CDS 45.0%
!! TTCACCGGCTTTCTTAGTAG+CGG - chr4.1:74204693-74204712 None:intergenic 45.0%
!!! ATTTTTGAGGCGGCGAATCA+TGG + chr4.1:74204854-74204873 MS.gene99045:CDS 45.0%
!!! CAAGCACTTTTGGAACGTAC+TGG + chr4.1:74204368-74204387 MS.gene99045:CDS 45.0%
AACTGCACATCCCCATTCCT+CGG - chr4.1:74205103-74205122 None:intergenic 50.0%
ACGGACACGTTTCTCCCAAA+CGG - chr4.1:74204183-74204202 None:intergenic 50.0%
AGCTGGTGTGAGGTATGCTA+TGG + chr4.1:74204487-74204506 MS.gene99045:CDS 50.0%
ATCACTAACAGGAACAGGCG+AGG - chr4.1:74205020-74205039 None:intergenic 50.0%
ATGCATTTGGCTGCTCAAGC+TGG + chr4.1:74204470-74204489 MS.gene99045:CDS 50.0%
CTCCAAAGACAAAGCTTCGC+CGG - chr4.1:74204047-74204066 None:intergenic 50.0%
CTGTTTATGGTCCTTGGGGT+AGG + chr4.1:74204774-74204793 MS.gene99045:CDS 50.0%
CTTACTTCAACCGTGTACGG+TGG + chr4.1:74204012-74204031 MS.gene99045:CDS 50.0%
GAATCATGGTACCGTTGCGA+GGG + chr4.1:74204868-74204887 MS.gene99045:CDS 50.0%
GGAGAGGCTGTTGAAGACTA+AGG + chr4.1:74205054-74205073 MS.gene99045:CDS 50.0%
GGATTCTCCGTTCTTGTGAC+CGG + chr4.1:74204230-74204249 MS.gene99045:CDS 50.0%
GTAGCGGCATACAAACTAGC+AGG - chr4.1:74204677-74204696 None:intergenic 50.0%
TTGAAAACCCTCAACTGCGC+CGG - chr4.1:74204986-74205005 None:intergenic 50.0%
! GCGTGCAAGACAAGCACTTT+TGG + chr4.1:74204358-74204377 MS.gene99045:CDS 50.0%
! TGGAAGTGGTGGGAAGAAGA+GGG + chr4.1:74204958-74204977 MS.gene99045:CDS 50.0%
!! GAGAAGAGTACTGGAAGTGG+TGG + chr4.1:74204947-74204966 MS.gene99045:CDS 50.0%
!! GCTGAGAAGAGTACTGGAAG+TGG + chr4.1:74204944-74204963 MS.gene99045:CDS 50.0%
AAACTGGGGTGGTCCCGTTT+GGG + chr4.1:74204166-74204185 MS.gene99045:CDS 55.0%
AGCAGCTCCGGTCACAAGAA+CGG - chr4.1:74204240-74204259 None:intergenic 55.0%
AGGAGTCCTTCAAACTGGGG+TGG + chr4.1:74204155-74204174 MS.gene99045:CDS 55.0%
CGAATCATGGTACCGTTGCG+AGG + chr4.1:74204867-74204886 MS.gene99045:CDS 55.0%
CGGACACGTTTCTCCCAAAC+GGG - chr4.1:74204182-74204201 None:intergenic 55.0%
GACGCCGGGAAAGTTCAAGA+TGG + chr4.1:74203980-74203999 MS.gene99045:CDS 55.0%
TCCCTAAAACACCGTCTCCG+CGG - chr4.1:74204301-74204320 None:intergenic 55.0%
TCCTCAATGACCGCCGTGAA+GGG - chr4.1:74204139-74204158 None:intergenic 55.0%
TCCTTGGGGTAGGCCTGATA+TGG + chr4.1:74204784-74204803 MS.gene99045:CDS 55.0%
! CTCCGGCGAAGCTTTGTCTT+TGG + chr4.1:74204042-74204061 MS.gene99045:CDS 55.0%
! CTGGAAGTGGTGGGAAGAAG+AGG + chr4.1:74204957-74204976 MS.gene99045:CDS 55.0%
! GAAGTGGTGGGAAGAAGAGG+GGG + chr4.1:74204960-74204979 MS.gene99045:CDS 55.0%
! GGAAGTGGTGGGAAGAAGAG+GGG + chr4.1:74204959-74204978 MS.gene99045:CDS 55.0%
! TTGAACTTTCCCGGCGTCGA+CGG - chr4.1:74203978-74203997 None:intergenic 55.0%
!! GAGGGGGTGTTTAGGTGCTT+TGG + chr4.1:74204916-74204935 MS.gene99045:CDS 55.0%
!! TAAACCGGTGACGGATCTGC+AGG + chr4.1:74205165-74205184 MS.gene99045:CDS 55.0%
ACCCTTCACGGCGGTCATTG+AGG + chr4.1:74204135-74204154 MS.gene99045:CDS 60.0%
ACCGGACTCTAGCGGAGGAA+CGG - chr4.1:74204202-74204221 None:intergenic 60.0%
ACGGGACCACCCCAGTTTGA+AGG - chr4.1:74204164-74204183 None:intergenic 60.0%
CAAACTGGGGTGGTCCCGTT+TGG + chr4.1:74204165-74204184 MS.gene99045:CDS 60.0%
CGGAGAATCCGTTCCGTGAC+CGG - chr4.1:74204220-74204239 None:intergenic 60.0%
CTCCTCAATGACCGCCGTGA+AGG - chr4.1:74204140-74204159 None:intergenic 60.0%
GAGGAGAAGTCGCCGGAGAA+CGG - chr4.1:74204106-74204125 None:intergenic 60.0%
GCCATATCAGGCCTACCCCA+AGG - chr4.1:74204788-74204807 None:intergenic 60.0%
GCTAGAGTCCGGTCACGGAA+CGG + chr4.1:74204209-74204228 MS.gene99045:CDS 60.0%
GTTCCGTGACCGGACTCTAG+CGG - chr4.1:74204210-74204229 None:intergenic 60.0%
GTTCTTGTGACCGGAGCTGC+TGG + chr4.1:74204239-74204258 MS.gene99045:CDS 60.0%
TAACAGTGCTCCGTCGACGC+CGG + chr4.1:74203965-74203984 MS.gene99045:CDS 60.0%
TCCGACGAAACCAGCAGCTC+CGG - chr4.1:74204252-74204271 None:intergenic 60.0%
TCCGGCGACTTCTCCTCTTC+CGG + chr4.1:74204109-74204128 MS.gene99045:CDS 60.0%
TCCGTTCCTCCGCTAGAGTC+CGG + chr4.1:74204198-74204217 MS.gene99045:CDS 60.0%
TGGCTGCTCAAGCTGGTGTG+AGG + chr4.1:74204477-74204496 MS.gene99045:CDS 60.0%
TGTACGGTGGCACGCTTCTC+CGG + chr4.1:74204025-74204044 MS.gene99045:CDS 60.0%
! GAGAAGCGTGCCACCGTACA+CGG - chr4.1:74204025-74204044 None:intergenic 60.0%
! GCCGCGGAGACGGTGTTTTA+GGG + chr4.1:74204297-74204316 MS.gene99045:CDS 60.0%
!! ACCGGAGCTGCTGGTTTCGT+CGG + chr4.1:74204248-74204267 MS.gene99045:CDS 60.0%
AACAGTGCTCCGTCGACGCC+GGG + chr4.1:74203966-74203985 MS.gene99045:CDS 65.0%
CCAGCCTGCAGATCCGTCAC+CGG - chr4.1:74205172-74205191 None:intergenic 65.0%
CCTCCGCTAGAGTCCGGTCA+CGG + chr4.1:74204204-74204223 MS.gene99045:CDS 65.0%
CTCTTCCGGCGGACCCTTCA+CGG + chr4.1:74204123-74204142 MS.gene99045:CDS 65.0%
GCCGGAAGAGGAGAAGTCGC+CGG - chr4.1:74204113-74204132 None:intergenic 65.0%
GCGCTTAAACGCCGCGGAGA+CGG + chr4.1:74204287-74204306 MS.gene99045:CDS 65.0%
GGCGACTTCTCCTCTTCCGG+CGG + chr4.1:74204112-74204131 MS.gene99045:CDS 65.0%
TCCGCAGCGCTTAAACGCCG+CGG + chr4.1:74204281-74204300 MS.gene99045:CDS 65.0%
TGGTGGGAAGAAGAGGGGGC+CGG + chr4.1:74204964-74204983 MS.gene99045:CDS 65.0%
! CGCCGCGGAGACGGTGTTTT+AGG + chr4.1:74204296-74204315 MS.gene99045:CDS 65.0%
! TCCGCGGCGTTTAAGCGCTG+CGG - chr4.1:74204285-74204304 None:intergenic 65.0%
CCGTGACCGGACTCTAGCGG+AGG - chr4.1:74204207-74204226 None:intergenic 70.0%
CGTGAAGGGTCCGCCGGAAG+AGG - chr4.1:74204125-74204144 None:intergenic 70.0%
TTCCGGCGGACCCTTCACGG+CGG + chr4.1:74204126-74204145 MS.gene99045:CDS 70.0%
!! CCGGTGACGGATCTGCAGGC+TGG + chr4.1:74205169-74205188 MS.gene99045:CDS 70.0%
AGAGGGGGCCGGCGCAGTTG+AGG + chr4.1:74204975-74204994 MS.gene99045:CDS 75.0%
GACCGCCGTGAAGGGTCCGC+CGG - chr4.1:74204131-74204150 None:intergenic 75.0%
GAGGGGGCCGGCGCAGTTGA+GGG + chr4.1:74204976-74204995 MS.gene99045:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 74203933 74205255 74203933 ID=MS.gene99045
chr4.1 mRNA 74203933 74205255 74203933 ID=MS.gene99045.t1;Parent=MS.gene99045
chr4.1 exon 74203933 74205255 74203933 ID=MS.gene99045.t1.exon1;Parent=MS.gene99045.t1
chr4.1 CDS 74203933 74205255 74203933 ID=cds.MS.gene99045.t1;Parent=MS.gene99045.t1
Gene Sequence

>MS.gene99045

ATGTCTCAGCTGAAGCAAATGTCGCACGCTGATAACAGTGCTCCGTCGACGCCGGGAAAGTTCAAGATGGAGAAAGCTTCTTACTTCAACCGTGTACGGTGGCACGCTTCTCCGGCGAAGCTTTGTCTTTGGAGTTTTGTTTTCTCAGCTGCGATCTTGATCTTCTTTTTCCGTTCTCCGGCGACTTCTCCTCTTCCGGCGGACCCTTCACGGCGGTCATTGAGGAGTCCTTCAAACTGGGGTGGTCCCGTTTGGGAGAAACGTGTCCGTTCCTCCGCTAGAGTCCGGTCACGGAACGGATTCTCCGTTCTTGTGACCGGAGCTGCTGGTTTCGTCGGAACACATGTCTCCGCAGCGCTTAAACGCCGCGGAGACGGTGTTTTAGGGATTGATAATTTCAATGACTATTATGACCCTTCTTTGAAGCGTGCAAGACAAGCACTTTTGGAACGTACTGGTGTTTTCATTGTTGAAGGTGATATCAATGATGCTGCTTTGTTAAGGAAGCTTTTTGAGGTTGTTCCTTTTACTCATGTCATGCATTTGGCTGCTCAAGCTGGTGTGAGGTATGCTATGGAGAATCCTGGTTCTTATGTTCATAGTAACATTGCTGGTTTTGTTAATTTGCTTGAGGTTTGTAAAAGTGTTAATCCTCAACCTTCAATTGTTTGGGCTAGTTCTAGTTCTGTTTATGGACTGAATACTAAGGTTCCTTTTTCTGAAAGGGATAGAACTGATCAACCTGCTAGTTTGTATGCCGCTACTAAGAAAGCCGGTGAAGAAATCGCGCATACTTATAATCATATCTATGGTCTTTCTTTAACTGGTTTGAGGTTTTTCACTGTTTATGGTCCTTGGGGTAGGCCTGATATGGCTTATTTCTTTTTCACTAGAGATATTTTGAAGGGGAAGACTATTCCTATTTTTGAGGCGGCGAATCATGGTACCGTTGCGAGGGATTTTACTTATATTGATGATATTGTGAGGGGGTGTTTAGGTGCTTTGGATACTGCTGAGAAGAGTACTGGAAGTGGTGGGAAGAAGAGGGGGCCGGCGCAGTTGAGGGTTTTCAATTTGGGGAATACCTCGCCTGTTCCTGTTAGTGATCTTGTGGGTATTTTGGAGAGGCTGTTGAAGACTAAGGCGAAGAGGAATATTATGAAGTTGCCGAGGAATGGGGATGTGCAGTTTACTCATGCGAATATTAGTTATGCACAGAGAGAGCTTGGTTATAAACCGGTGACGGATCTGCAGGCTGGTTTGAAGAAATTTGTGAGGTGGTATCTGAATTACTATTCTTCTGGGAAGAAAGCTGTTGAGTGA
Protein sequence

>MS.gene99045.t1

MSQLKQMSHADNSAPSTPGKFKMEKASYFNRVRWHASPAKLCLWSFVFSAAILIFFFRSPATSPLPADPSRRSLRSPSNWGGPVWEKRVRSSARVRSRNGFSVLVTGAAGFVGTHVSAALKRRGDGVLGIDNFNDYYDPSLKRARQALLERTGVFIVEGDINDAALLRKLFEVVPFTHVMHLAAQAGVRYAMENPGSYVHSNIAGFVNLLEVCKSVNPQPSIVWASSSSVYGLNTKVPFSERDRTDQPASLYAATKKAGEEIAHTYNHIYGLSLTGLRFFTVYGPWGRPDMAYFFFTRDILKGKTIPIFEAANHGTVARDFTYIDDIVRGCLGALDTAEKSTGSGGKKRGPAQLRVFNLGNTSPVPVSDLVGILERLLKTKAKRNIMKLPRNGDVQFTHANISYAQRELGYKPVTDLQAGLKKFVRWYLNYYSSGKKAVE