AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012685


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012685.t1 MTR_7g088160 43.769 329 146 3 34 337 75 389 4.36e-85 262
MS.gene012685.t1 MTR_4g063670 43.750 320 149 9 31 336 17 319 2.60e-63 204
MS.gene012685.t1 MTR_0867s0010 43.750 320 149 8 31 336 17 319 1.19e-59 194
MS.gene012685.t1 MTR_4g083510 39.763 337 169 10 16 336 2 320 1.49e-51 173
MS.gene012685.t1 MTR_7g107340 36.513 304 145 4 32 334 76 332 3.07e-50 171
MS.gene012685.t1 MTR_1g051185 47.682 151 69 4 93 241 45 187 5.94e-36 131
MS.gene012685.t1 MTR_3g069460 42.169 166 85 3 31 194 17 173 1.79e-35 127
MS.gene012685.t1 MTR_7g114170 33.993 303 124 8 32 334 61 287 2.41e-31 119
MS.gene012685.t1 MTR_6g081150 36.413 184 71 5 93 276 40 177 8.39e-25 99.4
MS.gene012685.t1 MTR_5g066280 24.503 302 200 5 36 335 55 330 3.05e-22 95.9
MS.gene012685.t1 MTR_3g067785 24.503 302 200 5 36 335 55 330 7.59e-22 94.7
MS.gene012685.t1 MTR_4g026120 24.503 302 200 5 36 335 55 330 8.22e-22 94.7
MS.gene012685.t1 MTR_4g036435 24.503 302 200 5 36 335 55 330 8.22e-22 94.7
MS.gene012685.t1 MTR_4g013220 24.503 302 200 5 36 335 55 330 8.30e-22 94.7
MS.gene012685.t1 MTR_2g022120 24.503 302 200 5 36 335 119 394 3.66e-21 93.6
MS.gene012685.t1 MTR_1g106885 24.503 302 200 5 36 335 116 391 4.33e-21 93.6
MS.gene012685.t1 MTR_5g077245 24.172 302 201 5 36 335 108 383 2.94e-20 90.9
MS.gene012685.t1 MTR_2g072860 28.659 164 112 2 34 197 29 187 6.37e-19 86.3
MS.gene012685.t1 MTR_7g461350 27.551 196 132 4 1 196 69 254 8.32e-19 86.7
MS.gene012685.t1 MTR_6g016670 27.551 196 132 4 1 196 69 254 8.32e-19 86.7
MS.gene012685.t1 MTR_3g464310 27.551 196 132 4 1 196 69 254 8.32e-19 86.7
MS.gene012685.t1 MTR_6g092533 27.329 161 114 1 36 196 119 276 1.00e-18 86.7
MS.gene012685.t1 MTR_3g464170 28.834 163 111 2 34 196 97 254 1.69e-18 85.9
MS.gene012685.t1 MTR_1g100375 28.834 163 111 2 34 196 97 254 1.69e-18 85.9
MS.gene012685.t1 MTR_6g051405 28.834 163 111 2 34 196 97 254 1.81e-18 85.9
MS.gene012685.t1 MTR_3g450670 28.221 163 112 2 34 196 93 250 4.19e-18 84.7
MS.gene012685.t1 MTR_1g471070 28.221 163 112 2 34 196 93 250 4.19e-18 84.7
MS.gene012685.t1 MTR_7g062380 23.977 171 112 1 75 245 26 178 3.99e-15 74.7
MS.gene012685.t1 MTR_6g453050 24.262 305 188 9 9 304 35 305 5.32e-14 72.0
MS.gene012685.t1 MTR_8g015740 37.931 116 56 3 2 105 19 130 3.09e-13 66.2
MS.gene012685.t1 MTR_4g046090 26.316 152 94 1 95 246 71 204 5.82e-13 68.6
MS.gene012685.t1 MTR_8g015750 33.645 214 94 8 141 336 3 186 6.79e-13 66.6
MS.gene012685.t1 MTR_7g083520 23.125 160 120 1 38 197 31 187 9.33e-13 68.2
MS.gene012685.t1 MTR_1g086750 27.966 118 85 0 75 192 26 143 1.49e-12 66.2
MS.gene012685.t1 MTR_4g046740 35.659 129 64 5 212 336 23 136 2.29e-12 63.9
MS.gene012685.t1 MTR_2g023560 63.636 44 16 0 74 117 62 105 3.93e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012685.t1 AT5G41220 38.889 198 108 4 66 254 214 407 1.17e-33 130
MS.gene012685.t1 AT5G41220 38.889 198 108 4 66 254 206 399 1.30e-33 130
MS.gene012685.t1 AT5G41220 38.889 198 108 4 66 254 264 457 1.55e-33 130
MS.gene012685.t1 AT5G41240 37.255 204 114 4 66 259 264 463 1.69e-32 127
MS.gene012685.t1 AT3G47680 32.558 215 117 8 38 243 14 209 7.56e-24 99.8
MS.gene012685.t1 AT1G66235 25.510 196 119 5 75 252 7 193 9.10e-13 68.2

Find 59 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGATTCTTCAGTAAAT+TGG 0.187266 8.1:+1385664 None:intergenic
GTAAATTGGCATGACCATTT+AGG 0.268868 8.1:+1385678 None:intergenic
TAGAAATATTTAACCATGAT+TGG 0.280172 8.1:+1385997 None:intergenic
ATCATGGTTAAATATTTCTA+AGG 0.308295 8.1:-1385994 MS.gene012685:CDS
TACTAAAAGATGAACCTAAA+TGG 0.349471 8.1:-1385692 MS.gene012685:CDS
TTCACTTGAGTTGTATTGTC+CGG 0.371973 8.1:+1385569 None:intergenic
AGAACAAAGAATTCTGCTTC+AGG 0.392497 8.1:-1385639 MS.gene012685:CDS
AGAATTCTTTGTTCTCTTGT+TGG 0.394331 8.1:+1385647 None:intergenic
GTTAGATCCACATGAACAAT+AGG 0.411169 8.1:+1385477 None:intergenic
TGGTCAAATGTGGGGATAGA+AGG 0.412319 8.1:+1386233 None:intergenic
TGAGCTTCACTATCTCTTGA+TGG 0.426003 8.1:-1385340 MS.gene012685:CDS
TCACTTGAGTTGTATTGTCC+GGG 0.435029 8.1:+1385570 None:intergenic
TCACCGCTTGTAGGCGTGTC+CGG 0.437280 8.1:+1385594 None:intergenic
AGTAGAGTGGTTCGTCCGAT+TGG 0.444657 8.1:-1385546 MS.gene012685:CDS
TGTCCGGGGTCACCGCTTGT+AGG 0.454198 8.1:+1385585 None:intergenic
CAATACTCATTGAAGTTCCT+TGG 0.455671 8.1:+1386213 None:intergenic
ATCTCTTGATGGATACATCT+AGG 0.458045 8.1:-1385329 MS.gene012685:CDS
ATACACTTCTTATCCAATCA+TGG 0.460631 8.1:-1386010 MS.gene012685:CDS
AGGAGAAAATATATAGAGAA+AGG 0.462201 8.1:-1386049 MS.gene012685:CDS
TTTGAAGAAATCTGAGCTGT+TGG 0.469253 8.1:+1386086 None:intergenic
ATGGTCGAAGGTGAACTTGA+TGG 0.477920 8.1:-1385856 MS.gene012685:CDS
CTACACATCCTCTTCTAATC+CGG 0.479246 8.1:-1385613 MS.gene012685:CDS
ATTTCTAAGGATCCAATTGT+GGG 0.492615 8.1:-1385981 MS.gene012685:CDS
GTAGAGTGGTTCGTCCGATT+GGG 0.497308 8.1:-1385545 MS.gene012685:CDS
GAAGGTGAGGATTGAAACAT+AGG 0.501482 8.1:+1386251 None:intergenic
TGAAGTTCCTTGGTCAAATG+TGG 0.502589 8.1:+1386223 None:intergenic
TTGTTCATGTGGATCTAACT+AGG 0.507003 8.1:-1385473 MS.gene012685:CDS
GACAAAAGCAGCTAAAAGAA+AGG 0.508350 8.1:-1385523 MS.gene012685:CDS
TATTTCTAAGGATCCAATTG+TGG 0.512026 8.1:-1385982 MS.gene012685:CDS
CCAACTTGAGAGGAAAATGA+TGG 0.520919 8.1:+1386143 None:intergenic
GCATGAAGGTAACAAGTTGA+AGG 0.526954 8.1:-1385394 MS.gene012685:CDS
CGACATTATGCAAGATGCAT+TGG 0.532468 8.1:-1385769 MS.gene012685:CDS
ACAAAAGCAGCTAAAAGAAA+GGG 0.543467 8.1:-1385522 MS.gene012685:CDS
GAAGTTCCTTGGTCAAATGT+GGG 0.548198 8.1:+1386224 None:intergenic
GCCTATTCAAGAAAGAAAAG+TGG 0.554764 8.1:-1385804 MS.gene012685:CDS
AGTTGGGTTAGATAATATTG+AGG 0.557841 8.1:-1386126 MS.gene012685:CDS
TGAATCAAATTAAATGTCGA+TGG 0.569722 8.1:-1385875 MS.gene012685:CDS
ACGCCTACAAGCGGTGACCC+CGG 0.571208 8.1:-1385588 MS.gene012685:CDS
TCATGTGGATCTAACTAGGA+TGG 0.578161 8.1:-1385469 MS.gene012685:CDS
TGAAACACCTATTGTTCATG+TGG 0.578577 8.1:-1385484 MS.gene012685:CDS
AATTAAATGTCGATGGTCGA+AGG 0.587022 8.1:-1385868 MS.gene012685:CDS
CGGATTAGAAGAGGATGTGT+AGG 0.590924 8.1:+1385614 None:intergenic
ATTATCTAACCCAACTTGAG+AGG 0.596041 8.1:+1386133 None:intergenic
TTCTAAGGATCCAATTGTGG+GGG 0.599880 8.1:-1385979 MS.gene012685:CDS
AAATGTGGGGATAGAAGGTG+AGG 0.601662 8.1:+1386238 None:intergenic
CACTTGAGTTGTATTGTCCG+GGG 0.607801 8.1:+1385571 None:intergenic
ATTGCACGTGGTGTGCATGA+AGG 0.607967 8.1:-1385408 MS.gene012685:CDS
GATAATTATAATCAATATCG+TGG 0.614936 8.1:-1385918 MS.gene012685:CDS
AAGTTCCTTGGTCAAATGTG+GGG 0.615231 8.1:+1386225 None:intergenic
AGGCGTGTCCGGATTAGAAG+AGG 0.638071 8.1:+1385605 None:intergenic
TTTCTAAGGATCCAATTGTG+GGG 0.639337 8.1:-1385980 MS.gene012685:CDS
CAACTCAAGTGAAAGTAGAG+TGG 0.640911 8.1:-1385559 MS.gene012685:CDS
AATCCGGACACGCCTACAAG+CGG 0.654384 8.1:-1385597 MS.gene012685:CDS
TCTATCCCCACATTTGACCA+AGG 0.657869 8.1:-1386230 MS.gene012685:CDS
ATATAGAGAAAGGTTTACCA+CGG 0.696539 8.1:-1386039 MS.gene012685:CDS
GCTTTAGAAGATATTGCACG+TGG 0.699659 8.1:-1385420 MS.gene012685:CDS
GGATACATCTAGGATGACAG+AGG 0.702928 8.1:-1385319 MS.gene012685:CDS
AAGAAGTGTATCTTCCTCCG+TGG 0.715732 8.1:+1386022 None:intergenic
TAGAGAAAGGTTTACCACGG+AGG 0.724762 8.1:-1386036 MS.gene012685:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTATAATCAATATCG+TGG - chr8.1:1385587-1385606 MS.gene012685:CDS 20.0%
!! TAGAAATATTTAACCATGAT+TGG + chr8.1:1385511-1385530 None:intergenic 20.0%
!! TTCAAAAGAAACAAAAACAA+AGG - chr8.1:1385436-1385455 MS.gene012685:CDS 20.0%
!!! ATCATGGTTAAATATTTCTA+AGG - chr8.1:1385511-1385530 MS.gene012685:CDS 20.0%
! AGGAGAAAATATATAGAGAA+AGG - chr8.1:1385456-1385475 MS.gene012685:CDS 25.0%
! GGAAAAAGAATTGCAAATTA+AGG - chr8.1:1386132-1386151 MS.gene012685:CDS 25.0%
! TACTAAAAGATGAACCTAAA+TGG - chr8.1:1385813-1385832 MS.gene012685:CDS 25.0%
! TGAATCAAATTAAATGTCGA+TGG - chr8.1:1385630-1385649 MS.gene012685:CDS 25.0%
ACAAAAGCAGCTAAAAGAAA+GGG - chr8.1:1385983-1386002 MS.gene012685:CDS 30.0%
AGAATTCTTTGTTCTCTTGT+TGG + chr8.1:1385861-1385880 None:intergenic 30.0%
ATACACTTCTTATCCAATCA+TGG - chr8.1:1385495-1385514 MS.gene012685:CDS 30.0%
ATATAGAGAAAGGTTTACCA+CGG - chr8.1:1385466-1385485 MS.gene012685:CDS 30.0%
TGTTGGATTCTTCAGTAAAT+TGG + chr8.1:1385844-1385863 None:intergenic 30.0%
! AGTTGGGTTAGATAATATTG+AGG - chr8.1:1385379-1385398 MS.gene012685:CDS 30.0%
! ATTTCTAAGGATCCAATTGT+GGG - chr8.1:1385524-1385543 MS.gene012685:CDS 30.0%
! TATTTCTAAGGATCCAATTG+TGG - chr8.1:1385523-1385542 MS.gene012685:CDS 30.0%
! TCTTTTAGTAATTTCCATGC+TGG + chr8.1:1385803-1385822 None:intergenic 30.0%
!! ACCACTTTTCTTTCTTGAAT+AGG + chr8.1:1385705-1385724 None:intergenic 30.0%
AATTAAATGTCGATGGTCGA+AGG - chr8.1:1385637-1385656 MS.gene012685:CDS 35.0%
AGAACAAAGAATTCTGCTTC+AGG - chr8.1:1385866-1385885 MS.gene012685:CDS 35.0%
ATTATCTAACCCAACTTGAG+AGG + chr8.1:1385375-1385394 None:intergenic 35.0%
CAATACTCATTGAAGTTCCT+TGG + chr8.1:1385295-1385314 None:intergenic 35.0%
GACAAAAGCAGCTAAAAGAA+AGG - chr8.1:1385982-1386001 MS.gene012685:CDS 35.0%
GCCTATTCAAGAAAGAAAAG+TGG - chr8.1:1385701-1385720 MS.gene012685:CDS 35.0%
GTAAATTGGCATGACCATTT+AGG + chr8.1:1385830-1385849 None:intergenic 35.0%
GTTAGATCCACATGAACAAT+AGG + chr8.1:1386031-1386050 None:intergenic 35.0%
TGAAACACCTATTGTTCATG+TGG - chr8.1:1386021-1386040 MS.gene012685:CDS 35.0%
TTGTTCATGTGGATCTAACT+AGG - chr8.1:1386032-1386051 MS.gene012685:CDS 35.0%
TTTGAAGAAATCTGAGCTGT+TGG + chr8.1:1385422-1385441 None:intergenic 35.0%
! ACCAAAGAGCAAGTTCTTTT+TGG - chr8.1:1385552-1385571 MS.gene012685:CDS 35.0%
! ATCTCTTGATGGATACATCT+AGG - chr8.1:1386176-1386195 MS.gene012685:CDS 35.0%
! CATCATTTTCCTCTCAAGTT+GGG - chr8.1:1385363-1385382 MS.gene012685:CDS 35.0%
! TCCAAAAAGAACTTGCTCTT+TGG + chr8.1:1385556-1385575 None:intergenic 35.0%
! TTCACTTGAGTTGTATTGTC+CGG + chr8.1:1385939-1385958 None:intergenic 35.0%
! TTTCTAAGGATCCAATTGTG+GGG - chr8.1:1385525-1385544 MS.gene012685:CDS 35.0%
AAGTTCCTTGGTCAAATGTG+GGG + chr8.1:1385283-1385302 None:intergenic 40.0%
CAACTCAAGTGAAAGTAGAG+TGG - chr8.1:1385946-1385965 MS.gene012685:CDS 40.0%
CCAACTTGAGAGGAAAATGA+TGG + chr8.1:1385365-1385384 None:intergenic 40.0%
CGACATTATGCAAGATGCAT+TGG - chr8.1:1385736-1385755 MS.gene012685:CDS 40.0%
CTACACATCCTCTTCTAATC+CGG - chr8.1:1385892-1385911 MS.gene012685:CDS 40.0%
GAAGTTCCTTGGTCAAATGT+GGG + chr8.1:1385284-1385303 None:intergenic 40.0%
TCATGTGGATCTAACTAGGA+TGG - chr8.1:1386036-1386055 MS.gene012685:CDS 40.0%
TGAAGTTCCTTGGTCAAATG+TGG + chr8.1:1385285-1385304 None:intergenic 40.0%
TGAGCTTCACTATCTCTTGA+TGG - chr8.1:1386165-1386184 MS.gene012685:CDS 40.0%
! CCATCATTTTCCTCTCAAGT+TGG - chr8.1:1385362-1385381 MS.gene012685:CDS 40.0%
! GCATGAAGGTAACAAGTTGA+AGG - chr8.1:1386111-1386130 MS.gene012685:CDS 40.0%
! GTTTTATGTTAGTGCCAGCA+TGG - chr8.1:1385786-1385805 MS.gene012685:CDS 40.0%
! TCACTTGAGTTGTATTGTCC+GGG + chr8.1:1385938-1385957 None:intergenic 40.0%
! TTCTAAGGATCCAATTGTGG+GGG - chr8.1:1385526-1385545 MS.gene012685:CDS 40.0%
!! GCTTTAGAAGATATTGCACG+TGG - chr8.1:1386085-1386104 MS.gene012685:CDS 40.0%
!!! TTAGCTGCTTTTGTCCCAAT+CGG + chr8.1:1385977-1385996 None:intergenic 40.0%
AAATGTGGGGATAGAAGGTG+AGG + chr8.1:1385270-1385289 None:intergenic 45.0%
ATGGTCGAAGGTGAACTTGA+TGG - chr8.1:1385649-1385668 MS.gene012685:CDS 45.0%
TAGAGAAAGGTTTACCACGG+AGG - chr8.1:1385469-1385488 MS.gene012685:CDS 45.0%
TCTATCCCCACATTTGACCA+AGG - chr8.1:1385275-1385294 MS.gene012685:CDS 45.0%
TGGTCAAATGTGGGGATAGA+AGG + chr8.1:1385275-1385294 None:intergenic 45.0%
! AAGAAGTGTATCTTCCTCCG+TGG + chr8.1:1385486-1385505 None:intergenic 45.0%
! CACTTGAGTTGTATTGTCCG+GGG + chr8.1:1385937-1385956 None:intergenic 45.0%
! CGGATTAGAAGAGGATGTGT+AGG + chr8.1:1385894-1385913 None:intergenic 45.0%
! GGATACATCTAGGATGACAG+AGG - chr8.1:1386186-1386205 MS.gene012685:CDS 45.0%
AGTAGAGTGGTTCGTCCGAT+TGG - chr8.1:1385959-1385978 MS.gene012685:CDS 50.0%
ATTGCACGTGGTGTGCATGA+AGG - chr8.1:1386097-1386116 MS.gene012685:CDS 50.0%
GTAGAGTGGTTCGTCCGATT+GGG - chr8.1:1385960-1385979 MS.gene012685:CDS 50.0%
!! CTTTGGTTTGCCCCCACAAT+TGG + chr8.1:1385539-1385558 None:intergenic 50.0%
AATCCGGACACGCCTACAAG+CGG - chr8.1:1385908-1385927 MS.gene012685:CDS 55.0%
! AGGCGTGTCCGGATTAGAAG+AGG + chr8.1:1385903-1385922 None:intergenic 55.0%
TCACCGCTTGTAGGCGTGTC+CGG + chr8.1:1385914-1385933 None:intergenic 60.0%
ACGCCTACAAGCGGTGACCC+CGG - chr8.1:1385917-1385936 MS.gene012685:CDS 65.0%
TGTCCGGGGTCACCGCTTGT+AGG + chr8.1:1385923-1385942 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 1385257 1386270 1385257 ID=MS.gene012685
chr8.1 mRNA 1385257 1386270 1385257 ID=MS.gene012685.t1;Parent=MS.gene012685
chr8.1 exon 1385257 1386270 1385257 ID=MS.gene012685.t1.exon1;Parent=MS.gene012685.t1
chr8.1 CDS 1385257 1386270 1385257 ID=cds.MS.gene012685.t1;Parent=MS.gene012685.t1
Gene Sequence

>MS.gene012685

ATGTTTCAATCCTCACCTTCTATCCCCACATTTGACCAAGGAACTTCAATGAGTATTGCTACAGAACAAGAAACAACATCTCAAGCGTCTCAACAAATTCAAAATCCATCATTTTCCTCTCAAGTTGGGTTAGATAATATTGAGGTTAATGATGAAGAAGAACCAACAGCTCAGATTTCTTCAAAAGAAACAAAAACAAAGGAGAAAATATATAGAGAAAGGTTTACCACGGAGGAAGATACACTTCTTATCCAATCATGGTTAAATATTTCTAAGGATCCAATTGTGGGGGCAAACCAAAGAGCAAGTTCTTTTTGGAAGAGAATTACAGATAATTATAATCAATATCGTGGTCATTTGAAAGAGAGAGAGTTGAATCAAATTAAATGTCGATGGTCGAAGGTGAACTTGATGGTTCATAAATTTGCAACTTGCTACAAACAAGCCTATTCAAGAAAGAAAAGTGGTAGCTCTGAAGACGACATTATGCAAGATGCATTGGCAATTTATGCTCATGATAAGCGTTCACGTTTTATGTTAGTGCCAGCATGGAAATTACTAAAAGATGAACCTAAATGGTCATGCCAATTTACTGAAGAATCCAACAAGAGAACAAAGAATTCTGCTTCAGGAGCCTACACATCCTCTTCTAATCCGGACACGCCTACAAGCGGTGACCCCGGACAATACAACTCAAGTGAAAGTAGAGTGGTTCGTCCGATTGGGACAAAAGCAGCTAAAAGAAAGGGAAAATCAAAAGCAAATGAAACACCTATTGTTCATGTGGATCTAACTAGGATGGAAGATGCACAACAATCAAAAGCTCTAGCTTTAGAAGATATTGCACGTGGTGTGCATGAAGGTAACAAGTTGAAGGAAAAAGAATTGCAAATTAAGGAAAGAAATGATGAGCTTCACTATCTCTTGATGGATACATCTAGGATGACAGAGGAACAAGTGCAAGCTCATAAAGTGTTGTGTAAAATTATTAGAGAAAAATATGAACTTGTGTAA

Protein sequence

>MS.gene012685.t1

MFQSSPSIPTFDQGTSMSIATEQETTSQASQQIQNPSFSSQVGLDNIEVNDEEEPTAQISSKETKTKEKIYRERFTTEEDTLLIQSWLNISKDPIVGANQRASSFWKRITDNYNQYRGHLKERELNQIKCRWSKVNLMVHKFATCYKQAYSRKKSGSSEDDIMQDALAIYAHDKRSRFMLVPAWKLLKDEPKWSCQFTEESNKRTKNSASGAYTSSSNPDTPTSGDPGQYNSSESRVVRPIGTKAAKRKGKSKANETPIVHVDLTRMEDAQQSKALALEDIARGVHEGNKLKEKELQIKERNDELHYLLMDTSRMTEEQVQAHKVLCKIIREKYELV