AlfalfaGEDB | MS.gene01288

M. sativa cultivar XinJiangDaYe / MS.gene01288

KEGG: -
GO: GO:0003674 GO:0004857 GO:0008150 GO:0008428 GO:0019219 GO:0019222 GO:0030234 GO:0031323 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0098772
PFAM: PF03737.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01288.t1	XP_013462438.1	97	166	5	0	1	166	1	166	4.10E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01288.t1	Q9FH13	75.3	166	41	0	1	166	1	166	4.3e-70	265.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01288.t1	A0A072V558	97.0	166	5	0	1	166	1	166	3.0e-88	334.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene51161	MS.gene01288	PPI
MS.gene033474	MS.gene01288	PPI
MS.gene52579	MS.gene01288	PPI
MS.gene024038	MS.gene01288	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01288.t1	MTR_2g010815	96.988	166	5	1	166	1	166	2.20e-118	331
MS.gene01288.t1	MTR_2g010800	86.747	166	22	1	166	1	166	8.59e-105	297
MS.gene01288.t1	MTR_7g080040	65.060	166	58	1	166	1	166	1.59e-80	236

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01288.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene01288.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene01288.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene01288.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene01288.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene01288.t1	AT3G02770	65.060	166	58	1	166	1	166	9.27e-78	229
MS.gene01288.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228
MS.gene01288.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228

Find 38 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCTTGTTGGAGGGAATT+TGG	0.199853	2.2:+70742056	MS.gene01288:CDS
TGGCCAGTCTCGGGCATTCT+CGG	0.281731	2.2:+70741921	MS.gene01288:CDS
GCACAAAACATGGGGTGGTC+TGG	0.304278	2.2:+70742087	MS.gene01288:CDS
GGCCAGTCTCGGGCATTCTC+GGG	0.351904	2.2:+70741922	MS.gene01288:CDS
TGGTCTGGGATTGTTGTGAA+TGG	0.381293	2.2:+70742102	MS.gene01288:CDS
AAAAGGAGAAGGAAGAGTTT+TGG	0.387441	2.2:+70742005	MS.gene01288:CDS
CACAAAACATGGGGTGGTCT+GGG	0.391877	2.2:+70742088	MS.gene01288:CDS
CCCGAGACTGGCCATACATC+TGG	0.415145	2.2:-70741912	None:intergenic
GCCCCGAGAATGCCCGAGAC+TGG	0.420007	2.2:-70741924	None:intergenic
TCAAGTTGGTGTTAGAGCTT+TGG	0.422544	2.2:+70742158	MS.gene01288:CDS
AACGCAACACATTTAACAAA+TGG	0.449158	2.2:+70741862	MS.gene01288:CDS
TCCAGATGTATGGCCAGTCT+CGG	0.458278	2.2:+70741911	MS.gene01288:CDS
GGTGCAGTTGGCACAAAACA+TGG	0.464361	2.2:+70742077	MS.gene01288:CDS
CATCCAGTGTTCCAGATGTA+TGG	0.472302	2.2:+70741901	MS.gene01288:CDS
CAGTCTAGTACTATTTCAAA+TGG	0.479945	2.2:-70742319	None:intergenic
TCAAATGGACAACTCAAACT+TGG	0.482072	2.2:-70742304	None:intergenic
TGGAGGGAATTTGGTGCAGT+TGG	0.484334	2.2:+70742065	MS.gene01288:CDS
AGAGAACTTCTTGAAACAAA+AGG	0.491488	2.2:+70741988	MS.gene01288:CDS
GAGATTAATTTGTGTCAAGT+TGG	0.491931	2.2:+70742144	MS.gene01288:CDS
CATGTTGCTGTTTATGTTGG+AGG	0.502059	2.2:+70742225	MS.gene01288:CDS
AAACATGTTGCTGTTTATGT+TGG	0.508506	2.2:+70742222	MS.gene01288:CDS
TGGCCATACATCTGGAACAC+TGG	0.512769	2.2:-70741904	None:intergenic
CCAGATGTATGGCCAGTCTC+GGG	0.532810	2.2:+70741912	MS.gene01288:CDS
GCCAGTCTCGGGCATTCTCG+GGG	0.553955	2.2:+70741923	MS.gene01288:CDS
AGCATGAGGTGTGCTCTTGT+TGG	0.557997	2.2:+70742045	MS.gene01288:CDS
ATGAGGTGTGCTCTTGTTGG+AGG	0.569564	2.2:+70742048	MS.gene01288:CDS
GTGTCACATATCTCTGCCGT+TGG	0.569988	2.2:-70741838	None:intergenic
CTTCTTGAAACAAAAGGAGA+AGG	0.585218	2.2:+70741994	MS.gene01288:CDS
TGGCTCTATGCAGATAATGA+TGG	0.590987	2.2:+70742270	MS.gene01288:CDS
ATTTAACAAATGGTGACTTG+AGG	0.616446	2.2:+70741872	MS.gene01288:CDS
AGTGTTTGAAGACAATGTGT+TGG	0.617013	2.2:+70741963	MS.gene01288:CDS
TAATATGGATTCTCTACCAA+CGG	0.621580	2.2:+70741822	None:intergenic
GTGCAGTTGGCACAAAACAT+GGG	0.623381	2.2:+70742078	MS.gene01288:CDS
GAATGGTTGTATTAGAGATG+TGG	0.623790	2.2:+70742119	MS.gene01288:CDS
TGAGGTGTGCTCTTGTTGGA+GGG	0.651891	2.2:+70742049	MS.gene01288:CDS
AGTTGGCACAAAACATGGGG+TGG	0.672135	2.2:+70742082	MS.gene01288:CDS
TGCAGTTGGCACAAAACATG+GGG	0.672279	2.2:+70742079	MS.gene01288:CDS
TTGATGGAGGAGGAAGCATG+AGG	0.673507	2.2:+70742031	MS.gene01288:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CCACTTAAATCCAATAAAAA+CGG	+	chr2.2:70742189-70742208	MS.gene01288:CDS	25.0%
!!	ATTGTCTTCAAACACTTTTA+CGG	-	chr2.2:70741959-70741978	None:intergenic	25.0%
	AAATCCAATAAAAACGGTAC+TGG	+	chr2.2:70742195-70742214	MS.gene01288:CDS	30.0%
	AACGCAACACATTTAACAAA+TGG	+	chr2.2:70741862-70741881	MS.gene01288:CDS	30.0%
	AGAGAACTTCTTGAAACAAA+AGG	+	chr2.2:70741988-70742007	MS.gene01288:CDS	30.0%
	ATTTAACAAATGGTGACTTG+AGG	+	chr2.2:70741872-70741891	MS.gene01288:CDS	30.0%
!!	AAACATGTTGCTGTTTATGT+TGG	+	chr2.2:70742222-70742241	MS.gene01288:CDS	30.0%
!!	CCGTTTTTATTGGATTTAAG+TGG	-	chr2.2:70742192-70742211	None:intergenic	30.0%
!!	GAGATTAATTTGTGTCAAGT+TGG	+	chr2.2:70742144-70742163	MS.gene01288:CDS	30.0%
	AGTGTTTGAAGACAATGTGT+TGG	+	chr2.2:70741963-70741982	MS.gene01288:CDS	35.0%
	CTTCTTGAAACAAAAGGAGA+AGG	+	chr2.2:70741994-70742013	MS.gene01288:CDS	35.0%
	GAATGGTTGTATTAGAGATG+TGG	+	chr2.2:70742119-70742138	MS.gene01288:CDS	35.0%
	TCAAATGGACAACTCAAACT+TGG	-	chr2.2:70742307-70742326	None:intergenic	35.0%
!	AAAAGGAGAAGGAAGAGTTT+TGG	+	chr2.2:70742005-70742024	MS.gene01288:CDS	35.0%
!	AACACTTTTACGGTAACAAC+AGG	-	chr2.2:70741949-70741968	None:intergenic	35.0%
!!	CTTTTATTCGTGATGGAGAA+TGG	+	chr2.2:70742250-70742269	MS.gene01288:CDS	35.0%
!!	TTCACCAGTACCGTTTTTAT+TGG	-	chr2.2:70742202-70742221	None:intergenic	35.0%
!!!	AGAGTTTTGGTTATTGATGG+AGG	+	chr2.2:70742018-70742037	MS.gene01288:CDS	35.0%
!!!	GGAAGAGTTTTGGTTATTGA+TGG	+	chr2.2:70742015-70742034	MS.gene01288:CDS	35.0%
!	GGAGGAACTTTTATTCGTGA+TGG	+	chr2.2:70742243-70742262	MS.gene01288:CDS	40.0%
!	TGGCTCTATGCAGATAATGA+TGG	+	chr2.2:70742270-70742289	MS.gene01288:CDS	40.0%
!!	CATGTTGCTGTTTATGTTGG+AGG	+	chr2.2:70742225-70742244	MS.gene01288:CDS	40.0%
!!	TCAAGTTGGTGTTAGAGCTT+TGG	+	chr2.2:70742158-70742177	MS.gene01288:CDS	40.0%
!!!	GTTTTGGTTATTGATGGAGG+AGG	+	chr2.2:70742021-70742040	MS.gene01288:CDS	40.0%
	CATCCAGTGTTCCAGATGTA+TGG	+	chr2.2:70741901-70741920	MS.gene01288:CDS	45.0%
	GTGCAGTTGGCACAAAACAT+GGG	+	chr2.2:70742078-70742097	MS.gene01288:CDS	45.0%
	TGCAGTTGGCACAAAACATG+GGG	+	chr2.2:70742079-70742098	MS.gene01288:CDS	45.0%
	TGCTCTTGTTGGAGGGAATT+TGG	+	chr2.2:70742056-70742075	MS.gene01288:CDS	45.0%
!	TGGTCTGGGATTGTTGTGAA+TGG	+	chr2.2:70742102-70742121	MS.gene01288:CDS	45.0%
	AGCATGAGGTGTGCTCTTGT+TGG	+	chr2.2:70742045-70742064	MS.gene01288:CDS	50.0%
	AGTTGGCACAAAACATGGGG+TGG	+	chr2.2:70742082-70742101	MS.gene01288:CDS	50.0%
	ATGAGGTGTGCTCTTGTTGG+AGG	+	chr2.2:70742048-70742067	MS.gene01288:CDS	50.0%
	CACAAAACATGGGGTGGTCT+GGG	+	chr2.2:70742088-70742107	MS.gene01288:CDS	50.0%
	GGTGCAGTTGGCACAAAACA+TGG	+	chr2.2:70742077-70742096	MS.gene01288:CDS	50.0%
	GTGTCACATATCTCTGCCGT+TGG	-	chr2.2:70741841-70741860	None:intergenic	50.0%
	TCCAGATGTATGGCCAGTCT+CGG	+	chr2.2:70741911-70741930	MS.gene01288:CDS	50.0%
	TGAGGTGTGCTCTTGTTGGA+GGG	+	chr2.2:70742049-70742068	MS.gene01288:CDS	50.0%
!	TGGCCATACATCTGGAACAC+TGG	-	chr2.2:70741907-70741926	None:intergenic	50.0%
!	TTGATGGAGGAGGAAGCATG+AGG	+	chr2.2:70742031-70742050	MS.gene01288:CDS	50.0%
!!	TGGAGGGAATTTGGTGCAGT+TGG	+	chr2.2:70742065-70742084	MS.gene01288:CDS	50.0%
	CCAGATGTATGGCCAGTCTC+GGG	+	chr2.2:70741912-70741931	MS.gene01288:CDS	55.0%
	GCACAAAACATGGGGTGGTC+TGG	+	chr2.2:70742087-70742106	MS.gene01288:CDS	55.0%
	TGGCCAGTCTCGGGCATTCT+CGG	+	chr2.2:70741921-70741940	MS.gene01288:CDS	60.0%
!	CCCGAGACTGGCCATACATC+TGG	-	chr2.2:70741915-70741934	None:intergenic	60.0%
	GCCAGTCTCGGGCATTCTCG+GGG	+	chr2.2:70741923-70741942	MS.gene01288:CDS	65.0%
	GGCCAGTCTCGGGCATTCTC+GGG	+	chr2.2:70741922-70741941	MS.gene01288:CDS	65.0%
	GCCCCGAGAATGCCCGAGAC+TGG	-	chr2.2:70741927-70741946	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	70741826	70742326	70741826	ID=MS.gene01288
chr2.2	mRNA	70741826	70742326	70741826	ID=MS.gene01288.t1;Parent=MS.gene01288
chr2.2	exon	70741826	70742326	70741826	ID=MS.gene01288.t1.exon1;Parent=MS.gene01288.t1
chr2.2	CDS	70741826	70742326	70741826	ID=cds.MS.gene01288.t1;Parent=MS.gene01288.t1

Gene Sequence

>MS.gene01288

ATGGATTCTCTACCAACGGCAGAGATATGTGACACAAACGCAACACATTTAACAAATGGTGACTTGAGGATACTTCATCCAGTGTTCCAGATGTATGGCCAGTCTCGGGCATTCTCGGGGCCTGTTGTTACCGTAAAAGTGTTTGAAGACAATGTGTTGGTAAGAGAACTTCTTGAAACAAAAGGAGAAGGAAGAGTTTTGGTTATTGATGGAGGAGGAAGCATGAGGTGTGCTCTTGTTGGAGGGAATTTGGTGCAGTTGGCACAAAACATGGGGTGGTCTGGGATTGTTGTGAATGGTTGTATTAGAGATGTGGATGAGATTAATTTGTGTCAAGTTGGTGTTAGAGCTTTGGCTTCTCATCCACTTAAATCCAATAAAAACGGTACTGGTGAAAAACATGTTGCTGTTTATGTTGGAGGAACTTTTATTCGTGATGGAGAATGGCTCTATGCAGATAATGATGGTATCATTGTATCCAAGTTTGAGTTGTCCATTTGA

Protein sequence

>MS.gene01288.t1

MDSLPTAEICDTNATHLTNGDLRILHPVFQMYGQSRAFSGPVVTVKVFEDNVLVRELLETKGEGRVLVIDGGGSMRCALVGGNLVQLAQNMGWSGIVVNGCIRDVDEINLCQVGVRALASHPLKSNKNGTGEKHVAVYVGGTFIRDGEWLYADNDGIIVSKFELSI