AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012942

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012942.t1 MTR_3g102220 94.421 466 21 2 1 462 10 474 0.0 869
MS.gene012942.t1 MTR_1g018840 71.674 466 124 3 2 462 12 474 0.0 679
MS.gene012942.t1 MTR_1g018840 72.644 435 111 3 2 431 12 443 0.0 637
MS.gene012942.t1 MTR_1g107550 65.668 434 140 4 17 447 26 453 0.0 572
MS.gene012942.t1 MTR_4g094918 65.169 356 104 4 5 356 9 348 1.38e-169 481
MS.gene012942.t1 MTR_3g112430 51.861 403 176 6 39 435 31 421 8.45e-135 396
MS.gene012942.t1 MTR_3g116080 52.047 342 154 4 10 351 18 349 5.78e-122 360
MS.gene012942.t1 MTR_3g075390 50.409 367 153 9 3 358 6 354 2.99e-119 353
MS.gene012942.t1 MTR_4g104370 50.556 360 164 8 8 362 6 356 1.84e-115 343
MS.gene012942.t1 MTR_2g016180 43.021 437 229 8 30 453 37 466 2.67e-115 347
MS.gene012942.t1 MTR_2g016270 42.563 437 216 9 30 453 37 451 3.00e-112 339
MS.gene012942.t1 MTR_4g080730 54.808 312 129 8 40 348 36 338 5.36e-112 334
MS.gene012942.t1 MTR_5g022560 52.351 319 137 7 37 348 32 342 7.45e-112 333
MS.gene012942.t1 MTR_4g079470 52.025 321 138 8 35 348 32 343 1.83e-111 333
MS.gene012942.t1 MTR_4g080700 54.662 311 131 7 40 348 36 338 1.91e-111 332
MS.gene012942.t1 MTR_4g079770 52.025 321 138 8 35 348 32 343 2.68e-111 332
MS.gene012942.t1 MTR_4g079440 47.175 354 172 6 1 349 1 344 1.12e-110 330
MS.gene012942.t1 MTR_4g047610 52.532 316 132 8 43 348 34 341 1.97e-110 330
MS.gene012942.t1 MTR_4g079800 51.402 321 140 8 35 348 32 343 1.62e-109 328
MS.gene012942.t1 MTR_4g107930 51.562 320 137 8 39 348 32 343 9.08e-109 326
MS.gene012942.t1 MTR_2g090890 51.282 312 142 4 40 350 35 337 3.47e-108 324
MS.gene012942.t1 MTR_2g075830 49.284 349 152 10 6 348 10 339 1.57e-103 312
MS.gene012942.t1 MTR_4g080360 49.521 313 143 6 44 349 37 341 5.23e-100 303
MS.gene012942.t1 MTR_2g083930 49.682 314 145 7 44 349 35 343 8.62e-99 300
MS.gene012942.t1 MTR_7g078600 49.355 310 139 10 44 349 42 337 2.38e-88 273
MS.gene012942.t1 MTR_7g079230 47.727 308 146 8 44 348 39 334 3.13e-88 273
MS.gene012942.t1 MTR_2g015850 49.667 300 136 10 56 349 73 363 1.98e-85 266
MS.gene012942.t1 MTR_4g065077 42.424 363 177 13 2 350 13 357 1.43e-79 251
MS.gene012942.t1 MTR_2g015920 38.482 369 205 13 1 357 8 366 1.10e-71 231
MS.gene012942.t1 MTR_8g086470 39.444 360 176 12 2 350 29 357 7.08e-69 224
MS.gene012942.t1 MTR_2g015930 37.912 364 199 13 2 350 8 359 8.05e-65 213
MS.gene012942.t1 MTR_4g125300 42.517 294 150 10 63 349 67 348 4.86e-64 211
MS.gene012942.t1 MTR_2g067900 41.304 322 162 13 44 349 16 326 1.33e-63 209
MS.gene012942.t1 MTR_1g023210 39.796 294 155 7 53 329 57 345 1.59e-63 210
MS.gene012942.t1 MTR_4g071060 36.994 346 190 8 1 329 7 341 2.11e-63 209
MS.gene012942.t1 MTR_2g067560 41.143 350 173 15 41 370 13 349 3.05e-60 212
MS.gene012942.t1 MTR_2g064475 37.249 349 164 15 41 347 13 348 9.11e-57 192
MS.gene012942.t1 MTR_1g023810 45.740 223 107 8 131 347 47 261 1.03e-53 181
MS.gene012942.t1 MTR_2g066980 37.538 325 142 14 44 348 34 317 2.51e-53 182
MS.gene012942.t1 MTR_1g094135 32.749 342 197 9 37 357 46 375 4.69e-51 177
MS.gene012942.t1 MTR_7g078630 39.796 294 127 9 23 304 690 945 1.54e-50 184
MS.gene012942.t1 MTR_4g072790 34.234 333 176 10 5 329 8 305 1.81e-48 169
MS.gene012942.t1 MTR_4g073660 34.030 335 178 9 5 329 7 308 5.78e-48 168
MS.gene012942.t1 MTR_4g072340 34.897 341 170 11 1 329 9 309 7.00e-48 167
MS.gene012942.t1 MTR_2g016380 39.381 226 119 5 230 439 9 232 1.05e-43 154
MS.gene012942.t1 MTR_7g078580 55.238 105 44 3 251 354 4 106 1.52e-31 117
MS.gene012942.t1 MTR_1g025450 41.379 174 80 5 131 301 53 207 3.52e-31 126
MS.gene012942.t1 MTR_7g079130 56.989 93 38 2 258 349 6 97 4.03e-28 107
MS.gene012942.t1 MTR_2g015840 39.259 135 77 3 36 168 25 156 1.85e-26 108
MS.gene012942.t1 MTR_2g015840 44.954 109 57 3 241 348 164 270 2.24e-26 108
MS.gene012942.t1 MTR_7g111060 27.358 318 187 13 58 341 23 330 6.43e-24 102
MS.gene012942.t1 MTR_3g029560 34.112 214 108 9 144 349 49 237 1.52e-20 90.9
MS.gene012942.t1 MTR_7g078310 40.559 143 74 6 86 225 113 247 1.91e-20 91.3
MS.gene012942.t1 MTR_7g111050 29.210 291 159 15 94 351 63 339 8.42e-20 90.9
MS.gene012942.t1 MTR_2g047960 48.750 80 41 0 141 220 6 85 4.60e-19 82.4
MS.gene012942.t1 MTR_8g087510 36.923 130 76 5 174 301 23 148 9.54e-17 78.2
MS.gene012942.t1 MTR_7g111050 29.032 248 136 13 94 310 58 296 3.74e-13 70.5
MS.gene012942.t1 MTR_7g079260 46.575 73 21 2 275 346 7 62 5.06e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012942.t1 AT1G47128 65.577 459 151 5 5 462 10 462 0.0 605
MS.gene012942.t1 AT5G43060 66.297 451 145 5 4 451 9 455 0.0 602
MS.gene012942.t1 AT3G19390 55.111 450 180 10 1 447 8 438 4.55e-173 493
MS.gene012942.t1 AT4G36880 62.029 345 124 4 18 355 23 367 3.39e-153 440
MS.gene012942.t1 AT1G09850 50.743 404 185 6 39 440 27 418 1.74e-141 412
MS.gene012942.t1 AT4G23520 54.261 352 147 7 1 350 11 350 1.92e-134 392
MS.gene012942.t1 AT3G19400 62.112 322 109 9 35 350 35 349 3.24e-134 391
MS.gene012942.t1 AT4G11310 54.108 353 152 6 1 350 8 353 3.89e-130 381
MS.gene012942.t1 AT4G11320 54.190 358 151 7 1 350 8 360 1.18e-129 380
MS.gene012942.t1 AT4G11320 54.190 358 151 7 1 350 8 360 1.18e-129 380
MS.gene012942.t1 AT3G48340 50.413 363 164 5 1 358 1 352 2.83e-122 361
MS.gene012942.t1 AT4G35350 50.286 350 165 4 1 350 13 353 7.02e-118 349
MS.gene012942.t1 AT1G20850 57.439 289 121 2 63 350 67 354 8.09e-117 347
MS.gene012942.t1 AT5G50260 53.354 328 140 6 36 357 30 350 6.43e-114 340
MS.gene012942.t1 AT3G48350 47.790 362 167 7 1 355 3 349 6.22e-110 329
MS.gene012942.t1 AT1G06260 47.714 350 164 6 2 350 12 343 7.06e-108 323
MS.gene012942.t1 AT5G45890 50.000 310 143 6 46 348 40 344 2.91e-104 314
MS.gene012942.t1 AT3G43960 52.083 336 147 12 33 361 30 358 2.24e-101 308
MS.gene012942.t1 AT3G19400 61.569 255 86 8 35 283 35 283 1.62e-97 295
MS.gene012942.t1 AT2G27420 43.094 362 174 11 1 349 5 347 2.65e-93 286
MS.gene012942.t1 AT3G49340 47.619 315 147 9 44 349 35 340 7.43e-93 285
MS.gene012942.t1 AT2G34080 42.210 353 178 8 4 349 11 344 6.85e-92 283
MS.gene012942.t1 AT4G35350 48.561 278 135 3 1 278 13 282 1.27e-85 265
MS.gene012942.t1 AT1G29080 39.718 355 191 10 3 349 6 345 2.07e-84 263
MS.gene012942.t1 AT1G29090 40.782 358 187 11 1 349 13 354 3.34e-82 258
MS.gene012942.t1 AT3G48350 44.637 289 140 5 1 283 3 277 1.29e-73 234
MS.gene012942.t1 AT1G29110 36.441 354 196 9 4 349 1 333 4.26e-71 228
MS.gene012942.t1 AT4G39090 38.870 301 159 7 44 327 51 343 4.52e-64 211
MS.gene012942.t1 AT5G60360 42.230 296 148 11 63 349 75 356 1.22e-63 210
MS.gene012942.t1 AT3G45310 42.321 293 152 8 63 349 75 356 1.31e-63 210
MS.gene012942.t1 AT3G45310 42.321 293 151 9 63 349 75 355 2.82e-61 204
MS.gene012942.t1 AT5G60360 41.497 294 152 10 63 349 75 355 3.01e-61 204
MS.gene012942.t1 AT5G60360 42.857 273 142 9 63 328 75 340 1.46e-60 202
MS.gene012942.t1 AT2G21430 34.012 344 199 8 1 327 8 340 4.35e-60 201
MS.gene012942.t1 AT4G16190 34.375 352 202 8 1 329 5 350 2.93e-56 191
MS.gene012942.t1 AT3G54940 33.129 326 196 7 22 329 26 347 2.07e-52 181
MS.gene012942.t1 AT3G54940 34.498 229 139 4 46 265 50 276 8.69e-38 139
MS.gene012942.t1 AT1G02305 28.070 285 167 13 85 341 59 333 2.30e-21 95.5
MS.gene012942.t1 AT4G01610 27.152 302 179 15 69 341 41 330 1.95e-19 90.1
MS.gene012942.t1 AT4G01610 26.490 302 181 15 69 341 41 330 1.23e-17 84.7
MS.gene012942.t1 AT1G02300 27.358 212 125 8 152 341 146 350 1.54e-14 75.5

Find 130 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAAAATGGAGTTGATTAT+TGG 0.228751 3.1:-81150167 MS.gene012942:CDS
GGAGGAGATGGTCCAGGATT+TGG 0.245379 3.1:+81150006 None:intergenic
CTGGTCTTTGTCCTTCCAAT+TGG 0.250747 3.1:+81152854 None:intergenic
GAGGAGATGGTCCAGGATTT+GGG 0.260610 3.1:+81150007 None:intergenic
GCTAAACTGCACTGGCCTTT+TGG 0.274256 3.1:-81149508 MS.gene012942:CDS
ATTCCTCCAATCAACAGATT+TGG 0.278810 3.1:+81152793 None:intergenic
CCTTCCAATTGGGTCAATCT+TGG 0.302486 3.1:+81152865 None:intergenic
TTCCTCCAATCAACAGATTT+GGG 0.337168 3.1:+81152794 None:intergenic
GTTGGGTATGGAACAGAAAA+TGG 0.361875 3.1:-81150180 MS.gene012942:CDS
AGGTTGCGATGGCGGACTTA+TGG 0.367299 3.1:-81151097 MS.gene012942:CDS
TGGTCTTTGTCCTTCCAATT+GGG 0.369130 3.1:+81152855 None:intergenic
CAGAGTAAGGATAATCCATT+TGG 0.374697 3.1:-81149556 MS.gene012942:CDS
TGTTGTTGTGTACTTGGAAT+TGG 0.377882 3.1:-81149922 MS.gene012942:CDS
AATTGCCCAAATCTGTTGAT+TGG 0.381743 3.1:-81152799 MS.gene012942:CDS
GGTAAGAGCTGGGGAGAATC+AGG 0.382333 3.1:-81150126 MS.gene012942:CDS
TCCATTGCCTTCACTCCAAA+TGG 0.390378 3.1:+81149541 None:intergenic
TTTCAAGGCTAACTCATCAT+AGG 0.392211 3.1:+81150838 None:intergenic
ACTACTTGTTGTTGTGTACT+TGG 0.392980 3.1:-81149928 MS.gene012942:CDS
TGATGAGTTAGCCTTGAAAA+AGG 0.393101 3.1:-81150834 MS.gene012942:CDS
GACCATGGTGTTACCGTTGT+TGG 0.394945 3.1:-81150198 MS.gene012942:CDS
AACTTCCTTGGTCTTTGACT+TGG 0.395186 3.1:+81152756 None:intergenic
TTCACCAAGGGACCTGTCTC+AGG 0.395806 3.1:-81149801 MS.gene012942:CDS
AACTTACGGATACATATAAC+TGG 0.399300 3.1:+81150759 None:intergenic
GGCAATTTGTCACCAACGCT+TGG 0.406197 3.1:+81152815 None:intergenic
GGAACCAAGATTGACCCAAT+TGG 0.414918 3.1:-81152869 MS.gene012942:CDS
CCCACGCAGACAAATCTTCA+TGG 0.415518 3.1:-81153114 MS.gene012942:CDS
ATTGGATAGTAAGAAATTCA+TGG 0.423583 3.1:-81150149 MS.gene012942:CDS
ACCATGGTGTTACCGTTGTT+GGG 0.427319 3.1:-81150197 MS.gene012942:CDS
GCTTCAATGGCAACACTGAT+TGG 0.431683 3.1:+81150797 None:intergenic
AGAGTACCGGTCTAGGTACT+TGG 0.432842 3.1:-81152891 MS.gene012942:CDS
AAAGGCCAGTGCAGTTTAGC+TGG 0.435726 3.1:+81149511 None:intergenic
TCAACAATTGCTGCAGTTGA+AGG 0.436695 3.1:-81151198 MS.gene012942:CDS
CATACAAGGTTGGGTTGAAC+CGG 0.439030 3.1:-81152934 MS.gene012942:CDS
TTCACTATCAGAACAAGAAC+TGG 0.442677 3.1:-81151145 MS.gene012942:CDS
GGTGTTACCGTTGTTGGGTA+TGG 0.442710 3.1:-81150192 MS.gene012942:CDS
TTCAATTGATGACTACGAAC+AGG 0.444920 3.1:-81150867 MS.gene012942:CDS
CTTCTCAGCAACATTTGCCT+TGG 0.448842 3.1:-81153164 MS.gene012942:CDS
AGAACTTACCACAACTTCCT+TGG 0.449633 3.1:+81152744 None:intergenic
TTCCCTACCACCAGCTTCAA+TGG 0.456425 3.1:+81150784 None:intergenic
TCAACCCAACCTTGTATGTC+CGG 0.460815 3.1:+81152939 None:intergenic
TATCCCTATCGTGGCGTTGA+TGG 0.462446 3.1:-81151021 MS.gene012942:CDS
GAAATTCATGGGGTAAGAGC+TGG 0.471932 3.1:-81150137 MS.gene012942:CDS
CCCTTGGTGAATGTTGCAAA+TGG 0.474942 3.1:+81149813 None:intergenic
GTCTTCCTATCCCATAAAGA+AGG 0.476257 3.1:-81150040 MS.gene012942:CDS
TTGGATAGTAAGAAATTCAT+GGG 0.479691 3.1:-81150148 MS.gene012942:CDS
TTGGCCCTTCTTTATGGGAT+AGG 0.486791 3.1:+81150035 None:intergenic
CGTTGCTAAGATCAGCGAAC+CGG 0.498812 3.1:+81152915 None:intergenic
TTACCCTGAGACAGGTCCCT+TGG 0.501613 3.1:+81149797 None:intergenic
ATGGGGATGTTGTCCTCTTG+AGG 0.503559 3.1:-81149884 MS.gene012942:CDS
ATCAGTGTTGCCATTGAAGC+TGG 0.504683 3.1:-81150794 MS.gene012942:CDS
GGTATATTCACAGGAAAATG+TGG 0.504752 3.1:-81150231 MS.gene012942:CDS
ACGAGCACAATGCTGAAAAC+CGG 0.505973 3.1:-81152958 MS.gene012942:CDS
AAATTCATGGGGTAAGAGCT+GGG 0.510565 3.1:-81150136 MS.gene012942:CDS
AAAACCGGACATACAAGGTT+GGG 0.510867 3.1:-81152943 MS.gene012942:CDS
ATCTCATTCTGACCTCCAAA+AGG 0.511131 3.1:+81149493 None:intergenic
TGCCCAAATCTGTTGATTGG+AGG 0.511810 3.1:-81152796 MS.gene012942:CDS
CAACAATTGCTGCAGTTGAA+GGG 0.513307 3.1:-81151197 MS.gene012942:CDS
CAACATAGACGAAAGCGAAA+AGG 0.520153 3.1:-81153023 MS.gene012942:CDS
CAAACGGTTGGTGGGTTCAC+AGG 0.523709 3.1:+81149979 None:intergenic
AGAATCAGGCTATGTAAGAA+TGG 0.528174 3.1:-81150112 MS.gene012942:CDS
CCATGAAGATTTGTCTGCGT+GGG 0.529942 3.1:+81153114 None:intergenic
ACTGTGATATCTACTACAAA+CGG 0.532542 3.1:+81149963 None:intergenic
AATCTAGCAACAAGCGTGGC+AGG 0.532543 3.1:-81150084 MS.gene012942:CDS
TCTGTTGATTGGAGGAATCA+AGG 0.534268 3.1:-81152788 MS.gene012942:CDS
TATCTACTACAAACGGTTGG+TGG 0.536068 3.1:+81149970 None:intergenic
TTTACCATCAACGCCACGAT+AGG 0.536522 3.1:+81151017 None:intergenic
TGCTGAAAACCGGACATACA+AGG 0.539893 3.1:-81152948 MS.gene012942:CDS
TGTAGCATTTACCCTGAGAC+AGG 0.542876 3.1:+81149789 None:intergenic
GTTGGTGGGTTCACAGGTGA+AGG 0.544235 3.1:+81149985 None:intergenic
GGATAATCCATTTGGAGTGA+AGG 0.546745 3.1:-81149548 MS.gene012942:CDS
TTTAAATGAAGGGAGCTGCT+GGG 0.548412 3.1:-81151226 MS.gene012942:intron
TTTGAGTTCATTATAAACAA+TGG 0.552648 3.1:-81151066 MS.gene012942:CDS
ATCTTAGCAACGAAGAGTAC+CGG 0.553613 3.1:-81152904 MS.gene012942:CDS
TGGAGCATAACGATTGCTCC+TGG 0.557522 3.1:+81152835 None:intergenic
TGTGCTCGTCTATGAACTTG+AGG 0.559695 3.1:+81152972 None:intergenic
GCAACGAAGAGTACCGGTCT+AGG 0.560358 3.1:-81152898 MS.gene012942:CDS
GTCAAAGACCAAGGAAGTTG+TGG 0.561678 3.1:-81152752 MS.gene012942:intron
GGGATTAACAAGATAGTCAC+AGG 0.565200 3.1:-81151177 MS.gene012942:CDS
GAAAACCGGACATACAAGGT+TGG 0.567992 3.1:-81152944 MS.gene012942:CDS
GGTGAAGGAGGAGATGGTCC+AGG 0.571654 3.1:+81150000 None:intergenic
GTTAATGCAGGTTGCGATGG+CGG 0.572329 3.1:-81151105 MS.gene012942:CDS
CAGCAGACTGCAGCCTCAAG+AGG 0.572739 3.1:+81149871 None:intergenic
TTACCATCAACGCCACGATA+GGG 0.574845 3.1:+81151018 None:intergenic
AAGGCTAACTCATCATAGGC+AGG 0.575627 3.1:+81150842 None:intergenic
AATCGTTATGCTCCAAGCGT+TGG 0.579034 3.1:-81152827 MS.gene012942:CDS
ATCTACTACAAACGGTTGGT+GGG 0.580238 3.1:+81149971 None:intergenic
TGCCATTGAAGCTGGTGGTA+GGG 0.580570 3.1:-81150786 MS.gene012942:CDS
ACAGTTAATGCAGGTTGCGA+TGG 0.581307 3.1:-81151108 MS.gene012942:CDS
ACAAGCGTGGCAGGTAAATG+TGG 0.582854 3.1:-81150075 MS.gene012942:CDS
ATTGTTTATAATGAACTCAA+AGG 0.583026 3.1:+81151068 None:intergenic
GAACTCCAGCTAAACTGCAC+TGG 0.583723 3.1:-81149516 MS.gene012942:CDS
TTCACAGGTGAAGGAGGAGA+TGG 0.584350 3.1:+81149994 None:intergenic
CCTTGGTGAATGTTGCAAAT+GGG 0.585245 3.1:+81149814 None:intergenic
GAGGAGTGGCGCGTGAAGCA+CGG 0.586775 3.1:-81153061 MS.gene012942:CDS
GGTGGGTTCACAGGTGAAGG+AGG 0.590702 3.1:+81149988 None:intergenic
GAGTTCATTATAAACAATGG+TGG 0.593504 3.1:-81151063 MS.gene012942:CDS
TGGTAAATGTGATCAATACA+AGG 0.597847 3.1:-81151001 MS.gene012942:intron
TCCATTTGGAGTGAAGGCAA+TGG 0.600766 3.1:-81149542 MS.gene012942:CDS
AGGAGATGGTCCAGGATTTG+GGG 0.608482 3.1:+81150008 None:intergenic
GAGTACCGGTCTAGGTACTT+GGG 0.611103 3.1:-81152890 MS.gene012942:CDS
TTCTGTTCCATACCCAACAA+CGG 0.611724 3.1:+81150185 None:intergenic
CCATTTGCAACATTCACCAA+GGG 0.613478 3.1:-81149813 MS.gene012942:CDS
CCAAGATTGACCCAATTGGA+AGG 0.613736 3.1:-81152865 MS.gene012942:CDS
TGTGACAGAACAGTTAATGC+AGG 0.614081 3.1:-81151117 MS.gene012942:CDS
GCCATGAAGATTTGTCTGCG+TGG 0.614353 3.1:+81153113 None:intergenic
TTGCCATTGAAGCTGGTGGT+AGG 0.615387 3.1:-81150787 MS.gene012942:CDS
TGATATCTACTACAAACGGT+TGG 0.615529 3.1:+81149967 None:intergenic
CCCATTTGCAACATTCACCA+AGG 0.618869 3.1:-81149814 MS.gene012942:CDS
TGGTTCCCAAGTACCTAGAC+CGG 0.622633 3.1:+81152885 None:intergenic
GGAAATGATAGACATATCCA+AGG 0.622761 3.1:+81153147 None:intergenic
ACGATCAAGATAAATAACAT+TGG 0.630041 3.1:+81153196 None:intergenic
TCTTCCTATCCCATAAAGAA+GGG 0.630892 3.1:-81150039 MS.gene012942:CDS
TTCATGGCGTAGTGACAAAG+AGG 0.632333 3.1:-81153098 MS.gene012942:CDS
CAATTGGAAGGACAAAGACC+AGG 0.635684 3.1:-81152853 MS.gene012942:CDS
TCACCAAGGGACCTGTCTCA+GGG 0.637588 3.1:-81149800 MS.gene012942:intron
TAAAGACTATGTACGAGGAG+TGG 0.644931 3.1:-81153075 MS.gene012942:CDS
GTATATTCACAGGAAAATGT+GGG 0.649822 3.1:-81150230 MS.gene012942:CDS
TTGCAAATGGGATAGTCATG+AGG 0.655589 3.1:+81149826 None:intergenic
AATTCATGGGGTAAGAGCTG+GGG 0.658294 3.1:-81150135 MS.gene012942:CDS
ACCCAACAACGGTAACACCA+TGG 0.674055 3.1:+81150196 None:intergenic
GTGGTCCAAGTCAAAGACCA+AGG 0.674979 3.1:-81152761 MS.gene012942:CDS
TTGGAGGAATCAAGGTGCTG+TGG 0.684642 3.1:-81152780 MS.gene012942:CDS
TGGTGGTATTGACAGCGATG+AGG 0.693529 3.1:-81151046 MS.gene012942:CDS
GATGAGGATTATCCCTATCG+TGG 0.699238 3.1:-81151030 MS.gene012942:CDS
GGAGATGGTCCAGGATTTGG+GGG 0.699912 3.1:+81150009 None:intergenic
TGTGGGACAGCATTAGACCA+TGG 0.705102 3.1:-81150213 MS.gene012942:CDS
TGGATAGTAAGAAATTCATG+GGG 0.710961 3.1:-81150147 MS.gene012942:CDS
ACGTAATCTAGCAACAAGCG+TGG 0.711518 3.1:-81150088 MS.gene012942:CDS
AGTGTTGCCATTGAAGCTGG+TGG 0.717173 3.1:-81150791 MS.gene012942:CDS
AGAGGTAAAGACTATGTACG+AGG 0.762500 3.1:-81153080 MS.gene012942:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTATTTTTTTAACC+CGG + chr3.1:81151262-81151281 None:intergenic 10.0%
!!! TTTTTTCTTTTTTAAATGAA+GGG - chr3.1:81151437-81151456 MS.gene012942:intron 10.0%
!!! TTTTTTTCTTTTTTAAATGA+AGG - chr3.1:81151436-81151455 MS.gene012942:intron 10.0%
!! AAATTAAATGACTTATAATG+TGG + chr3.1:81150538-81150557 None:intergenic 15.0%
!! ATTATTAAACTTATAGTGTT+TGG + chr3.1:81150979-81150998 None:intergenic 15.0%
!! CCTTTCAAAAAAAAAAAAAA+CGG + chr3.1:81150930-81150949 None:intergenic 15.0%
!! TGAAAGATAATATAAAAAAG+TGG + chr3.1:81150841-81150860 None:intergenic 15.0%
!! TGAAAGATAATTTAAAAAAG+TGG + chr3.1:81150734-81150753 None:intergenic 15.0%
!!! AGTCAAATAATTTTTTTGAT+AGG + chr3.1:81152256-81152275 None:intergenic 15.0%
!!! TACTGATTAGATTAAATATA+AGG + chr3.1:81152060-81152079 None:intergenic 15.0%
!!! TACTTATTTGTTTTTAACAA+AGG - chr3.1:81151161-81151180 MS.gene012942:CDS 15.0%
!!! TATTTTTTACATTATGGTTA+AGG - chr3.1:81151063-81151082 MS.gene012942:CDS 15.0%
!! AAAAAAGAAAAAAAGAGTAG+AGG + chr3.1:81151430-81151449 None:intergenic 20.0%
!! AAGTTAGAATAAGAAACTTA+CGG + chr3.1:81151931-81151950 None:intergenic 20.0%
!! ACACGTAATAGATATAAATA+TGG + chr3.1:81150496-81150515 None:intergenic 20.0%
!! AGAAAACTATAAGCAATTAT+TGG + chr3.1:81151752-81151771 None:intergenic 20.0%
!! ATAGCTCATAAAATTAATGA+AGG - chr3.1:81151316-81151335 MS.gene012942:intron 20.0%
!! ATAGTGTTTGGAAAAATTAA+CGG + chr3.1:81150967-81150986 None:intergenic 20.0%
!! ATTGTTTATAATGAACTCAA+AGG + chr3.1:81151608-81151627 None:intergenic 20.0%
!! CCATAATGTAAAAAATACAA+CGG + chr3.1:81151060-81151079 None:intergenic 20.0%
!! TAAATATGCACTATTTGAAT+TGG - chr3.1:81153085-81153104 MS.gene012942:CDS 20.0%
!! TAGCTCATAAAATTAATGAA+GGG - chr3.1:81151317-81151336 MS.gene012942:intron 20.0%
!! TTCAATCAATCACATTAAAT+AGG - chr3.1:81150633-81150652 MS.gene012942:intron 20.0%
!!! AAATTTTGCAGCATATTATT+TGG - chr3.1:81152983-81153002 MS.gene012942:CDS 20.0%
!!! AATTTTGCAGCATATTATTT+GGG - chr3.1:81152984-81153003 MS.gene012942:CDS 20.0%
!!! AATTTTTGTAAATCACTCTA+TGG + chr3.1:81150701-81150720 None:intergenic 20.0%
!!! CCGTTTTTTTTTTTTTTGAA+AGG - chr3.1:81150927-81150946 MS.gene012942:intron 20.0%
!!! GATAATATTGTTTGCTAAAT+AGG - chr3.1:81150761-81150780 MS.gene012942:intron 20.0%
!!! GATAGAATTTTATGTTTTCA+AGG - chr3.1:81150401-81150420 MS.gene012942:intron 20.0%
!!! GGTATTTTTAGAGATATTTT+AGG - chr3.1:81150654-81150673 MS.gene012942:intron 20.0%
!!! TATTTGTTTTTAACAAAGGT+TGG - chr3.1:81151165-81151184 MS.gene012942:CDS 20.0%
!!! TTACTATTGATTATCCAAAA+TGG + chr3.1:81151229-81151248 None:intergenic 20.0%
!!! TTCTTCAATTTTTGATTGTA+GGG - chr3.1:81150120-81150139 MS.gene012942:CDS 20.0%
!!! TTTAGAATAGTATCAACAAT+TGG + chr3.1:81150064-81150083 None:intergenic 20.0%
!!! TTTGAGTTCATTATAAACAA+TGG - chr3.1:81151607-81151626 MS.gene012942:intron 20.0%
!!! TTTTTTTACAATTGATCTAG+TGG - chr3.1:81150154-81150173 MS.gene012942:CDS 20.0%
! AATCAGTACATATGTGAATT+TGG - chr3.1:81152072-81152091 MS.gene012942:intron 25.0%
! AATGAGAACAAGTAATTGTA+GGG - chr3.1:81150248-81150267 MS.gene012942:intron 25.0%
! ATTGGATAGTAAGAAATTCA+TGG - chr3.1:81152524-81152543 MS.gene012942:intron 25.0%
! GTACTGTATGAATTATGATT+AGG + chr3.1:81151983-81152002 None:intergenic 25.0%
! GTTATTCACAAGAATTTACA+AGG - chr3.1:81152311-81152330 MS.gene012942:intron 25.0%
! GTTTCTTATTCTAACTTACT+CGG - chr3.1:81151934-81151953 MS.gene012942:intron 25.0%
! TATTCACCGAAAAAAAATGA+AGG + chr3.1:81151379-81151398 None:intergenic 25.0%
! TGATTAACAGCATAAGTATT+TGG - chr3.1:81152160-81152179 MS.gene012942:intron 25.0%
! TTCTCATTGTTACAATCTAA+TGG + chr3.1:81150236-81150255 None:intergenic 25.0%
! TTGGATAGTAAGAAATTCAT+GGG - chr3.1:81152525-81152544 MS.gene012942:intron 25.0%
!! AATAGTAATTGACATTTTCC+GGG - chr3.1:81151241-81151260 MS.gene012942:intron 25.0%
!! ATTTTATAATGCCAATAACC+TGG - chr3.1:81151351-81151370 MS.gene012942:intron 25.0%
!! ATTTTGCAGCATATTATTTG+GGG - chr3.1:81152985-81153004 MS.gene012942:CDS 25.0%
!! CAATAGTAATTGACATTTTC+CGG - chr3.1:81151240-81151259 MS.gene012942:intron 25.0%
!! TACTTATCACAATTTTTGCA+GGG - chr3.1:81152421-81152440 MS.gene012942:intron 25.0%
!!! AATTTTTGTAAAGCACTCTA+TGG + chr3.1:81150808-81150827 None:intergenic 25.0%
!!! CCGTTGTATTTTTTACATTA+TGG - chr3.1:81151057-81151076 MS.gene012942:CDS 25.0%
!!! GCATTAAGTTGATTATATTG+AGG - chr3.1:81150280-81150299 MS.gene012942:intron 25.0%
!!! GTTCTTCAATTTTTGATTGT+AGG - chr3.1:81150119-81150138 MS.gene012942:CDS 25.0%
!!! TGACACTTTTGTTAGTTTAA+AGG + chr3.1:81152952-81152971 None:intergenic 25.0%
!!! TTTTTTTTTTTTTGTGCGTG+TGG - chr3.1:81149987-81150006 MS.gene012942:CDS 25.0%
AAAAATCTACCATCAAATGC+TGG + chr3.1:81150332-81150351 None:intergenic 30.0%
AACAGCATAAGTATTTGGTA+AGG - chr3.1:81152165-81152184 MS.gene012942:intron 30.0%
AACTTACGGATACATATAAC+TGG + chr3.1:81151917-81151936 None:intergenic 30.0%
AATGCTGGAATTATTCACTT+AGG + chr3.1:81150317-81150336 None:intergenic 30.0%
ACACATATATATGACACACT+TGG - chr3.1:81152910-81152929 MS.gene012942:CDS 30.0%
ACAGCATAAGTATTTGGTAA+GGG - chr3.1:81152166-81152185 MS.gene012942:intron 30.0%
ACTGTGATATCTACTACAAA+CGG + chr3.1:81152713-81152732 None:intergenic 30.0%
ACTTACAGTTGAAAGAGATT+AGG + chr3.1:81152098-81152117 None:intergenic 30.0%
ATGCAAAGTTAAGTCTTCAA+TGG + chr3.1:81153055-81153074 None:intergenic 30.0%
CAATGAGAACAAGTAATTGT+AGG - chr3.1:81150247-81150266 MS.gene012942:intron 30.0%
GAGTTCATTATAAACAATGG+TGG - chr3.1:81151610-81151629 MS.gene012942:intron 30.0%
GTATATTCACAGGAAAATGT+GGG - chr3.1:81152443-81152462 MS.gene012942:intron 30.0%
TACTCTAACAAAGTGAATGA+GGG + chr3.1:81150036-81150055 None:intergenic 30.0%
TCATTTCTGCACATTCTTAT+TGG - chr3.1:81152133-81152152 MS.gene012942:intron 30.0%
TGAATAATTCCAGCATTTGA+TGG - chr3.1:81150320-81150339 MS.gene012942:intron 30.0%
TGCAAAGTTAAGTCTTCAAT+GGG + chr3.1:81153054-81153073 None:intergenic 30.0%
TGGATAGTAAGAAATTCATG+GGG - chr3.1:81152526-81152545 MS.gene012942:intron 30.0%
TGGTAAATGTGATCAATACA+AGG - chr3.1:81151672-81151691 MS.gene012942:intron 30.0%
TTACTCTAACAAAGTGAATG+AGG + chr3.1:81150037-81150056 None:intergenic 30.0%
! CAGAAAATGGAGTTGATTAT+TGG - chr3.1:81152506-81152525 MS.gene012942:intron 30.0%
! CTACTTATCACAATTTTTGC+AGG - chr3.1:81152420-81152439 MS.gene012942:intron 30.0%
! CTATTGATTATCCAAAATGG+TGG + chr3.1:81151226-81151245 None:intergenic 30.0%
!! TGGAAAACTTTGCTTTTCAT+GGG - chr3.1:81152771-81152790 MS.gene012942:CDS 30.0%
!! TTGGAAAACTTTGCTTTTCA+TGG - chr3.1:81152770-81152789 MS.gene012942:CDS 30.0%
!!! CTGGTTCCTTCATTTTTTTT+CGG - chr3.1:81151370-81151389 MS.gene012942:intron 30.0%
!!! GAATTGCTTTCTTTGTTTTC+TGG - chr3.1:81152386-81152405 MS.gene012942:intron 30.0%
!!! TAGGAGCTTTTATTTTACTC+CGG + chr3.1:81151964-81151983 None:intergenic 30.0%
!!! TTTCTTTGTTTTCTGGCTTT+AGG - chr3.1:81152393-81152412 MS.gene012942:intron 30.0%
AAATGAAGGAACCAGGTTAT+TGG + chr3.1:81151365-81151384 None:intergenic 35.0%
AATTGCCCAAATCTGTTGAT+TGG - chr3.1:81149874-81149893 MS.gene012942:CDS 35.0%
AGAATCAGGCTATGTAAGAA+TGG - chr3.1:81152561-81152580 MS.gene012942:intron 35.0%
AGTAATTGTAGGGACTAAAG+TGG - chr3.1:81150258-81150277 MS.gene012942:intron 35.0%
ATATGACACACTTGGCATTA+TGG - chr3.1:81152918-81152937 MS.gene012942:CDS 35.0%
ATTCATACAGTACGAGTAGA+AGG - chr3.1:81151990-81152009 MS.gene012942:intron 35.0%
ATTCCTCCAATCAACAGATT+TGG + chr3.1:81149883-81149902 None:intergenic 35.0%
CAGAGTAAGGATAATCCATT+TGG - chr3.1:81153117-81153136 MS.gene012942:CDS 35.0%
CGAAAAAAAATGAAGGAACC+AGG + chr3.1:81151372-81151391 None:intergenic 35.0%
CTAGCAAATGTACTTTCAGA+AGG + chr3.1:81152337-81152356 None:intergenic 35.0%
GGAAATGATAGACATATCCA+AGG + chr3.1:81149529-81149548 None:intergenic 35.0%
GGTATATTCACAGGAAAATG+TGG - chr3.1:81152442-81152461 MS.gene012942:intron 35.0%
TCTTCCTATCCCATAAAGAA+GGG - chr3.1:81152634-81152653 MS.gene012942:intron 35.0%
TGAAATCTCGAATTCGAACT+CGG + chr3.1:81150190-81150209 None:intergenic 35.0%
TGACTTATAATGTGGCAATG+TGG + chr3.1:81150530-81150549 None:intergenic 35.0%
TGATATCTACTACAAACGGT+TGG + chr3.1:81152709-81152728 None:intergenic 35.0%
TGTTGTTGTGTACTTGGAAT+TGG - chr3.1:81152751-81152770 MS.gene012942:intron 35.0%
TTCACTATCAGAACAAGAAC+TGG - chr3.1:81151528-81151547 MS.gene012942:intron 35.0%
TTCATACAGTACGAGTAGAA+GGG - chr3.1:81151991-81152010 MS.gene012942:intron 35.0%
TTCCTCCAATCAACAGATTT+GGG + chr3.1:81149882-81149901 None:intergenic 35.0%
TTGGTTTATCTTGCAGAGTA+AGG - chr3.1:81153104-81153123 MS.gene012942:CDS 35.0%
TTTCAAGGCTAACTCATCAT+AGG + chr3.1:81151838-81151857 None:intergenic 35.0%
! TTAACAAAGGTTGGTTGAGT+TGG - chr3.1:81151174-81151193 MS.gene012942:CDS 35.0%
! TTCAATTGATGACTACGAAC+AGG - chr3.1:81151806-81151825 MS.gene012942:intron 35.0%
! TTTGATTGTAGGGACTCTTT+TGG - chr3.1:81150130-81150149 MS.gene012942:CDS 35.0%
! TTTTTGCAGGGTATATTCAC+AGG - chr3.1:81152433-81152452 MS.gene012942:intron 35.0%
!! ACTACTTGTTGTTGTGTACT+TGG - chr3.1:81152745-81152764 MS.gene012942:intron 35.0%
!! ATTAGCTACAGCCTTTTTCA+AGG + chr3.1:81151853-81151872 None:intergenic 35.0%
!! GGAAAACTTTGCTTTTCATG+GGG - chr3.1:81152772-81152791 MS.gene012942:CDS 35.0%
!! TGATGAGTTAGCCTTGAAAA+AGG - chr3.1:81151839-81151858 MS.gene012942:intron 35.0%
AAAACCGGACATACAAGGTT+GGG - chr3.1:81149730-81149749 MS.gene012942:intron 40.0%
AAATTCATGGGGTAAGAGCT+GGG - chr3.1:81152537-81152556 MS.gene012942:intron 40.0%
AGAACTTACCACAACTTCCT+TGG + chr3.1:81149932-81149951 None:intergenic 40.0%
AGAGGTAAAGACTATGTACG+AGG - chr3.1:81149593-81149612 MS.gene012942:intron 40.0%
ATCTACTACAAACGGTTGGT+GGG + chr3.1:81152705-81152724 None:intergenic 40.0%
ATCTCATTCTGACCTCCAAA+AGG + chr3.1:81153183-81153202 None:intergenic 40.0%
CAACAATTGCTGCAGTTGAA+GGG - chr3.1:81151476-81151495 MS.gene012942:intron 40.0%
CAACATAGACGAAAGCGAAA+AGG - chr3.1:81149650-81149669 MS.gene012942:intron 40.0%
CCATTTGCAACATTCACCAA+GGG - chr3.1:81152860-81152879 MS.gene012942:CDS 40.0%
CCTAAGAAGTTAGAAGTCAG+AGG - chr3.1:81152192-81152211 MS.gene012942:intron 40.0%
CCTCTGACTTCTAACTTCTT+AGG + chr3.1:81152195-81152214 None:intergenic 40.0%
CCTTGGTGAATGTTGCAAAT+GGG + chr3.1:81152862-81152881 None:intergenic 40.0%
CTTATAATGTGGCAATGTGG+TGG + chr3.1:81150527-81150546 None:intergenic 40.0%
GAAATCTCGAATTCGAACTC+GGG + chr3.1:81150189-81150208 None:intergenic 40.0%
GGCTAGAGTTAGTAAGTTAG+TGG + chr3.1:81152235-81152254 None:intergenic 40.0%
GGGATTAACAAGATAGTCAC+AGG - chr3.1:81151496-81151515 MS.gene012942:intron 40.0%
GTCTTCCTATCCCATAAAGA+AGG - chr3.1:81152633-81152652 MS.gene012942:intron 40.0%
GTTGGGTATGGAACAGAAAA+TGG - chr3.1:81152493-81152512 MS.gene012942:intron 40.0%
TAAAGACTATGTACGAGGAG+TGG - chr3.1:81149598-81149617 MS.gene012942:intron 40.0%
TATCTACTACAAACGGTTGG+TGG + chr3.1:81152706-81152725 None:intergenic 40.0%
TCAACAATTGCTGCAGTTGA+AGG - chr3.1:81151475-81151494 MS.gene012942:intron 40.0%
TGGTCTTTGTCCTTCCAATT+GGG + chr3.1:81149821-81149840 None:intergenic 40.0%
TGTGACAGAACAGTTAATGC+AGG - chr3.1:81151556-81151575 MS.gene012942:intron 40.0%
TTCTGTTCCATACCCAACAA+CGG + chr3.1:81152491-81152510 None:intergenic 40.0%
TTGCAAATGGGATAGTCATG+AGG + chr3.1:81152850-81152869 None:intergenic 40.0%
TTTAAATGAAGGGAGCTGCT+GGG - chr3.1:81151447-81151466 MS.gene012942:intron 40.0%
! AACTTCCTTGGTCTTTGACT+TGG + chr3.1:81149920-81149939 None:intergenic 40.0%
! ATCTTAGCAACGAAGAGTAC+CGG - chr3.1:81149769-81149788 MS.gene012942:intron 40.0%
! ATTTTCCCTGTCAGTTGAGT+TGG + chr3.1:81151135-81151154 None:intergenic 40.0%
! GGATAATCCATTTGGAGTGA+AGG - chr3.1:81153125-81153144 MS.gene012942:CDS 40.0%
! TCTGTTGATTGGAGGAATCA+AGG - chr3.1:81149885-81149904 MS.gene012942:CDS 40.0%
! TTTTAAATGAAGGGAGCTGC+TGG - chr3.1:81151446-81151465 MS.gene012942:intron 40.0%
!! GGATTTTGGCCCTTCTTTAT+GGG + chr3.1:81152646-81152665 None:intergenic 40.0%
AAGGCTAACTCATCATAGGC+AGG + chr3.1:81151834-81151853 None:intergenic 45.0%
AATCGTTATGCTCCAAGCGT+TGG - chr3.1:81149846-81149865 MS.gene012942:CDS 45.0%
AATTCATGGGGTAAGAGCTG+GGG - chr3.1:81152538-81152557 MS.gene012942:intron 45.0%
ACAGTTAATGCAGGTTGCGA+TGG - chr3.1:81151565-81151584 MS.gene012942:intron 45.0%
ACGAGCACAATGCTGAAAAC+CGG - chr3.1:81149715-81149734 MS.gene012942:intron 45.0%
ACGTAATCTAGCAACAAGCG+TGG - chr3.1:81152585-81152604 MS.gene012942:intron 45.0%
ATAATGTGGCAATGTGGTGG+AGG + chr3.1:81150524-81150543 None:intergenic 45.0%
ATCAGTGTTGCCATTGAAGC+TGG - chr3.1:81151879-81151898 MS.gene012942:intron 45.0%
CAATTGGAAGGACAAAGACC+AGG - chr3.1:81149820-81149839 MS.gene012942:CDS 45.0%
CCAAGATTGACCCAATTGGA+AGG - chr3.1:81149808-81149827 MS.gene012942:CDS 45.0%
CCATGAAGATTTGTCTGCGT+GGG + chr3.1:81149562-81149581 None:intergenic 45.0%
CCCATTTGCAACATTCACCA+AGG - chr3.1:81152859-81152878 MS.gene012942:CDS 45.0%
CCCTTGGTGAATGTTGCAAA+TGG + chr3.1:81152863-81152882 None:intergenic 45.0%
CCTTCCAATTGGGTCAATCT+TGG + chr3.1:81149811-81149830 None:intergenic 45.0%
CTTCTCAGCAACATTTGCCT+TGG - chr3.1:81149509-81149528 MS.gene012942:CDS 45.0%
GAAAACCGGACATACAAGGT+TGG - chr3.1:81149729-81149748 MS.gene012942:intron 45.0%
GAAATTCATGGGGTAAGAGC+TGG - chr3.1:81152536-81152555 MS.gene012942:intron 45.0%
GATGAGGATTATCCCTATCG+TGG - chr3.1:81151643-81151662 MS.gene012942:intron 45.0%
GCTTCAATGGCAACACTGAT+TGG + chr3.1:81151879-81151898 None:intergenic 45.0%
GGAACCAAGATTGACCCAAT+TGG - chr3.1:81149804-81149823 MS.gene012942:CDS 45.0%
GTCAAAGACCAAGGAAGTTG+TGG - chr3.1:81149921-81149940 MS.gene012942:CDS 45.0%
TACCACCAACTCAACTGACA+GGG - chr3.1:81151127-81151146 MS.gene012942:CDS 45.0%
TCAACCCAACCTTGTATGTC+CGG + chr3.1:81149737-81149756 None:intergenic 45.0%
TCCATTGCCTTCACTCCAAA+TGG + chr3.1:81153135-81153154 None:intergenic 45.0%
TGCTGAAAACCGGACATACA+AGG - chr3.1:81149725-81149744 MS.gene012942:intron 45.0%
TGTAGCATTTACCCTGAGAC+AGG + chr3.1:81152887-81152906 None:intergenic 45.0%
TGTGCTCGTCTATGAACTTG+AGG + chr3.1:81149704-81149723 None:intergenic 45.0%
TTACCATCAACGCCACGATA+GGG + chr3.1:81151658-81151677 None:intergenic 45.0%
TTAGTGGTTACGCTGAATCG+TGG + chr3.1:81152219-81152238 None:intergenic 45.0%
TTCATGGCGTAGTGACAAAG+AGG - chr3.1:81149575-81149594 MS.gene012942:intron 45.0%
TTGGCCCTTCTTTATGGGAT+AGG + chr3.1:81152641-81152660 None:intergenic 45.0%
TTTACCATCAACGCCACGAT+AGG + chr3.1:81151659-81151678 None:intergenic 45.0%
! CTGGTCTTTGTCCTTCCAAT+TGG + chr3.1:81149822-81149841 None:intergenic 45.0%
! TCCATTTGGAGTGAAGGCAA+TGG - chr3.1:81153131-81153150 MS.gene012942:CDS 45.0%
! TGCCCAAATCTGTTGATTGG+AGG - chr3.1:81149877-81149896 MS.gene012942:CDS 45.0%
!! AAAGGTTGGTTGAGTTGGTG+AGG - chr3.1:81151179-81151198 MS.gene012942:CDS 45.0%
!! ACCATGGTGTTACCGTTGTT+GGG - chr3.1:81152476-81152495 MS.gene012942:intron 45.0%
!! CATACAAGGTTGGGTTGAAC+CGG - chr3.1:81149739-81149758 MS.gene012942:intron 45.0%
!! GGGATTTTGGCCCTTCTTTA+TGG + chr3.1:81152647-81152666 None:intergenic 45.0%
!!! ACATTTTTAAAATTTTAAAT+AGG - chr3.1:81151281-81151300 MS.gene012942:intron 5.0%
AAAGGCCAGTGCAGTTTAGC+TGG + chr3.1:81153165-81153184 None:intergenic 50.0%
AATCTAGCAACAAGCGTGGC+AGG - chr3.1:81152589-81152608 MS.gene012942:intron 50.0%
ACAAGCGTGGCAGGTAAATG+TGG - chr3.1:81152598-81152617 MS.gene012942:intron 50.0%
ACCCAACAACGGTAACACCA+TGG + chr3.1:81152480-81152499 None:intergenic 50.0%
AGGAGATGGTCCAGGATTTG+GGG + chr3.1:81152668-81152687 None:intergenic 50.0%
AGTGTTGCCATTGAAGCTGG+TGG - chr3.1:81151882-81151901 MS.gene012942:intron 50.0%
ATGGGGATGTTGTCCTCTTG+AGG - chr3.1:81152789-81152808 MS.gene012942:CDS 50.0%
CAAAATGGTGGTCTTCTCGC+TGG + chr3.1:81151214-81151233 None:intergenic 50.0%
CCCACGCAGACAAATCTTCA+TGG - chr3.1:81149559-81149578 MS.gene012942:intron 50.0%
CGTTGCTAAGATCAGCGAAC+CGG + chr3.1:81149761-81149780 None:intergenic 50.0%
GAACTCCAGCTAAACTGCAC+TGG - chr3.1:81153157-81153176 MS.gene012942:CDS 50.0%
GAGGAGATGGTCCAGGATTT+GGG + chr3.1:81152669-81152688 None:intergenic 50.0%
GCCATGAAGATTTGTCTGCG+TGG + chr3.1:81149563-81149582 None:intergenic 50.0%
GGCAATTTGTCACCAACGCT+TGG + chr3.1:81149861-81149880 None:intergenic 50.0%
GTACCACCAACTCAACTGAC+AGG - chr3.1:81151126-81151145 MS.gene012942:CDS 50.0%
GTGGTCCAAGTCAAAGACCA+AGG - chr3.1:81149912-81149931 MS.gene012942:CDS 50.0%
GTTAATGCAGGTTGCGATGG+CGG - chr3.1:81151568-81151587 MS.gene012942:intron 50.0%
TGCCATTGAAGCTGGTGGTA+GGG - chr3.1:81151887-81151906 MS.gene012942:intron 50.0%
TGGAGCATAACGATTGCTCC+TGG + chr3.1:81149841-81149860 None:intergenic 50.0%
TGGTTCCCAAGTACCTAGAC+CGG + chr3.1:81149791-81149810 None:intergenic 50.0%
TGTGGGACAGCATTAGACCA+TGG - chr3.1:81152460-81152479 MS.gene012942:intron 50.0%
TTCACAGGTGAAGGAGGAGA+TGG + chr3.1:81152682-81152701 None:intergenic 50.0%
TTCCCTACCACCAGCTTCAA+TGG + chr3.1:81151892-81151911 None:intergenic 50.0%
TTGCCATTGAAGCTGGTGGT+AGG - chr3.1:81151886-81151905 MS.gene012942:intron 50.0%
! CAGCGAGAAGACCACCATTT+TGG - chr3.1:81151212-81151231 MS.gene012942:CDS 50.0%
! GGTGTTACCGTTGTTGGGTA+TGG - chr3.1:81152481-81152500 MS.gene012942:intron 50.0%
! GTCCAGGATTTGGGGGATTT+TGG + chr3.1:81152660-81152679 None:intergenic 50.0%
! TATCCCTATCGTGGCGTTGA+TGG - chr3.1:81151652-81151671 MS.gene012942:intron 50.0%
! TTCTAACTTACTCGGCTCGC+CGG - chr3.1:81151942-81151961 MS.gene012942:intron 50.0%
!! AGAGTACCGGTCTAGGTACT+TGG - chr3.1:81149782-81149801 MS.gene012942:intron 50.0%
!! GACCATGGTGTTACCGTTGT+TGG - chr3.1:81152475-81152494 MS.gene012942:intron 50.0%
!! GAGTACCGGTCTAGGTACTT+GGG - chr3.1:81149783-81149802 MS.gene012942:intron 50.0%
!! GTCTGCGTGGGTTTTGTCAT+AGG + chr3.1:81149550-81149569 None:intergenic 50.0%
!! TGGTGGTATTGACAGCGATG+AGG - chr3.1:81151627-81151646 MS.gene012942:intron 50.0%
!! TTCCCTGTCAGTTGAGTTGG+TGG + chr3.1:81151132-81151151 None:intergenic 50.0%
!! TTGGAGGAATCAAGGTGCTG+TGG - chr3.1:81149893-81149912 MS.gene012942:CDS 50.0%
!!! AAACTGCACTGGCCTTTTGG+AGG - chr3.1:81153168-81153187 MS.gene012942:CDS 50.0%
!!! GCTAAACTGCACTGGCCTTT+TGG - chr3.1:81153165-81153184 MS.gene012942:CDS 50.0%
GGAGATGGTCCAGGATTTGG+GGG + chr3.1:81152667-81152686 None:intergenic 55.0%
GGAGGAGATGGTCCAGGATT+TGG + chr3.1:81152670-81152689 None:intergenic 55.0%
GGCCAAAATCCCCCAAATCC+TGG - chr3.1:81152655-81152674 MS.gene012942:intron 55.0%
GGTAAGAGCTGGGGAGAATC+AGG - chr3.1:81152547-81152566 MS.gene012942:intron 55.0%
TCACCAAGGGACCTGTCTCA+GGG - chr3.1:81152873-81152892 MS.gene012942:CDS 55.0%
TTACCCTGAGACAGGTCCCT+TGG + chr3.1:81152879-81152898 None:intergenic 55.0%
TTCACCAAGGGACCTGTCTC+AGG - chr3.1:81152872-81152891 MS.gene012942:CDS 55.0%
!! AGGTTGCGATGGCGGACTTA+TGG - chr3.1:81151576-81151595 MS.gene012942:intron 55.0%
!! CAAACGGTTGGTGGGTTCAC+AGG + chr3.1:81152697-81152716 None:intergenic 55.0%
!! GCAACGAAGAGTACCGGTCT+AGG - chr3.1:81149775-81149794 MS.gene012942:intron 55.0%
!! GTTGGTGGGTTCACAGGTGA+AGG + chr3.1:81152691-81152710 None:intergenic 55.0%
CAGCAGACTGCAGCCTCAAG+AGG + chr3.1:81152805-81152824 None:intergenic 60.0%
GGTGAAGGAGGAGATGGTCC+AGG + chr3.1:81152676-81152695 None:intergenic 60.0%
! GGTGGGTTCACAGGTGAAGG+AGG + chr3.1:81152688-81152707 None:intergenic 60.0%
GAGGAGTGGCGCGTGAAGCA+CGG - chr3.1:81149612-81149631 MS.gene012942:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 81149480 81153215 81149480 ID=MS.gene012942
chr3.1 mRNA 81149480 81153215 81149480 ID=MS.gene012942.t1;Parent=MS.gene012942
chr3.1 exon 81152753 81153215 81152753 ID=MS.gene012942.t1.exon1;Parent=MS.gene012942.t1
chr3.1 CDS 81152753 81153215 81152753 ID=cds.MS.gene012942.t1;Parent=MS.gene012942.t1
chr3.1 exon 81151002 81151237 81151002 ID=MS.gene012942.t1.exon2;Parent=MS.gene012942.t1
chr3.1 CDS 81151002 81151237 81151002 ID=cds.MS.gene012942.t1;Parent=MS.gene012942.t1
chr3.1 exon 81150766 81150906 81150766 ID=MS.gene012942.t1.exon3;Parent=MS.gene012942.t1
chr3.1 CDS 81150766 81150906 81150766 ID=cds.MS.gene012942.t1;Parent=MS.gene012942.t1
chr3.1 exon 81149801 81150253 81149801 ID=MS.gene012942.t1.exon4;Parent=MS.gene012942.t1
chr3.1 CDS 81149801 81150253 81149801 ID=cds.MS.gene012942.t1;Parent=MS.gene012942.t1
chr3.1 exon 81149480 81149575 81149480 ID=MS.gene012942.t1.exon5;Parent=MS.gene012942.t1
chr3.1 CDS 81149480 81149575 81149480 ID=cds.MS.gene012942.t1;Parent=MS.gene012942.t1
Gene Sequence

>MS.gene012942

ATGTTATTTATCTTGATCGTGTTCACGCTCTTCTCAGCAACATTTGCCTTGGATATGTCTATCATTTCCTATGACAAAACCCACGCAGACAAATCTTCATGGCGTAGTGACAAAGAGGTAAAGACTATGTACGAGGAGTGGCGCGTGAAGCACGGAAAGCTCAATAACAACAACATAGACGAAAGCGAAAAGGATAAGAGATTTGAAATCTTCAAAGACAACCTCAAGTTCATAGACGAGCACAATGCTGAAAACCGGACATACAAGGTTGGGTTGAACCGGTTCGCTGATCTTAGCAACGAAGAGTACCGGTCTAGGTACTTGGGAACCAAGATTGACCCAATTGGAAGGACAAAGACCAGGAGCAATCGTTATGCTCCAAGCGTTGGTGACAAATTGCCCAAATCTGTTGATTGGAGGAATCAAGGTGCTGTGGTCCAAGTCAAAGACCAAGGAAGTTGTGGTAAGTTCTACTCGTTTAACTATTGTCTTTGTGTACTTTTTTTTTTTTTTTTTTTTTGTGCGTGTGGTTACATTGTCTTATTATGTTAGTCCCTCATTCACTTTGTTAGAGTAAATATCCAATTGTTGATACTATTCTAAAATGATTTTTGAAATTGTAAAATTTAAATTAAATTCGTTCTTCAATTTTTGATTGTAGGGACTCTTTTGGCTTTTTTTACAATTGATCTAGTGGCTAGTACATCCCGAGTTCGAATTCGAGATTTCACATAAAATATACTCGCAATTAAACCATTAGATTGTAACAATGAGAACAAGTAATTGTAGGGACTAAAGTGGCATTAAGTTGATTATATTGAGGATGAGTTCGAACCTAAGTGAATAATTCCAGCATTTGATGGTAGATTTTTAATATTTCATGAATCGTTTTAGAACATTGTTAATATGATTGCTTAGATTGATAGAATTTTATGTTTTCAAGGACTAAAACAATTATGTTTATATGTATAAATTTTTGTAAAAATATTAATTGATGTTCAAAAATATCAAGTCCATATTTATATCTATTACGTGTTACTTCCTCCACCACATTGCCACATTATAAGTCATTTAATTTTTTTTAATAAATTATAAATCATTTAAAATAGAGATTAATTTCTATACATCGACATAAAACTTTTTTGCATATGCATTCAATCAATCACATTAAATAGGTATTTTTAGAGATATTTTAGGAAATAAACTAAAAATGTAGCACCATAGAGTGATTTACAAAAATTATGAATTATTCCACTTTTTTAAATTATCTTTCATTAGATAGATAATATTGTTTGCTAAATAGGAAATAAACTAAAAATGTAGCACCATAGAGTGCTTTACAAAAATTATGAATTATTCCACTTTTTTATATTATCTTTCATTAGATAATATTGTTTGCTAAAATAATTTATATCACGAGACTTATAATATTTATCATTTAGTTTAACCGTTTTTTTTTTTTTTGAAAGGATCATTTAGTTTAACCGTTAATTTTTCCAAACACTATAAGTTTAATAATTAACTTATCTACTATTTGTTATCTGCTATAAGATAGCTTATTTTTTTATCAAACAGAGCCGTTGTATTTTTTACATTATGGTTAAGGTAGACACTAAACATGAAATGTTTCTAAATGAGTAAATTTTGTACCACCAACTCAACTGACAGGGAAAATGTCGATTACTTATTTGTTTTTAACAAAGGTTGGTTGAGTTGGTGAGGAGTTCAACTCCAGCGAGAAGACCACCATTTTGGATAATCAATAGTAATTGACATTTTCCGGGTTAAAAAAATAAAATTAACATTTTTAAAATTTTAAATAGGATAAATTTTAGAATAGCTCATAAAATTAATGAAGGGTGTGAATATATATTTTATAATGCCAATAACCTGGTTCCTTCATTTTTTTTCGGTGAATATAGATACTGTTCTGCAACTAACTGAGATCCTCTACTCTTTTTTTCTTTTTTAAATGAAGGGAGCTGCTGGGCTTTTTCAACAATTGCTGCAGTTGAAGGGATTAACAAGATAGTCACAGGAGAGCTTGTTTCACTATCAGAACAAGAACTGGTTGATTGTGACAGAACAGTTAATGCAGGTTGCGATGGCGGACTTATGGAATATGCCTTTGAGTTCATTATAAACAATGGTGGTATTGACAGCGATGAGGATTATCCCTATCGTGGCGTTGATGGTAAATGTGATCAATACAAGGTTAGTTAGTCTTCATTTTTTGATGAATTAACAACTGCTTGTAATATTTCTCACTCCAATAATTGCTTATAGTTTTCTCTGCTGTCTTGTCTCAGAAAAATGCTAGAGTTGTTTCAATTGATGACTACGAACAGGTTCCTGCCTATGATGAGTTAGCCTTGAAAAAGGCTGTAGCTAATCAGCCAATCAGTGTTGCCATTGAAGCTGGTGGTAGGGAATTCCAGTTATATGTATCCGTAAGTTTCTTATTCTAACTTACTCGGCTCGCCGGAGTAAAATAAAAGCTCCTAATCATAATTCATACAGTACGAGTAGAAGGGAAAGAGTCGAAACTAGTAATGCATGCTAAAGCATAAAATTGTGCCTTATATTTAATCTAATCAGTACATATGTGAATTTGGCCTAATCTCTTTCAACTGTAAGTTAGATTGATATGTAATCATTTCTGCACATTCTTATTGGACTATGATTAACAGCATAAGTATTTGGTAAGGGACACCTAAGAAGTTAGAAGTCAGAGGTCCACGATTCAGCGTAACCACTAACTTACTAACTCTAGCCTATCAAAAAAATTATTTGACTATGATACTTGAATTATTATTGTTGTTGTTATTTCTGTTATTCACAAGAATTTACAAGGCCTTCTGAAAGTACATTTGCTAGACTCTCTTGTACGTATAACGTATCTATGTGAATTGCTTTCTTTGTTTTCTGGCTTTAGGTATTCTACTTATCACAATTTTTGCAGGGTATATTCACAGGAAAATGTGGGACAGCATTAGACCATGGTGTTACCGTTGTTGGGTATGGAACAGAAAATGGAGTTGATTATTGGATAGTAAGAAATTCATGGGGTAAGAGCTGGGGAGAATCAGGCTATGTAAGAATGGAACGTAATCTAGCAACAAGCGTGGCAGGTAAATGTGGAATTGCAATGCAGTCTTCCTATCCCATAAAGAAGGGCCAAAATCCCCCAAATCCTGGACCATCTCCTCCTTCACCTGTGAACCCACCAACCGTTTGTAGTAGATATCACAGTTGTGCTTCAAGCACTACTTGTTGTTGTGTACTTGGAATTGGAAAACTTTGCTTTTCATGGGGATGTTGTCCTCTTGAGGCTGCAGTCTGCTGTAAAGATCACTCAAGTTGTTGTCCTCATGACTATCCCATTTGCAACATTCACCAAGGGACCTGTCTCAGGGTAAATGCTACACACACACATATATATGACACACTTGGCATTATGGCATATTTTCCTTTAAACTAACAAAAGTGTCAGAAAACGTGTTAAATTTTGCAGCATATTATTTGGGGAAGACTTTTTCTTAGAATAGATAGATAAATATATACTGAGTTTCCCATTGAAGACTTAACTTTGCATTATCACTAAATAAATATGCACTATTTGAATTGGTTTATCTTGCAGAGTAAGGATAATCCATTTGGAGTGAAGGCAATGGAGAGAACTCCAGCTAAACTGCACTGGCCTTTTGGAGGTCAGAATGAGATAAATATTGCTTAA
Protein sequence

>MS.gene012942.t1

MLFILIVFTLFSATFALDMSIISYDKTHADKSSWRSDKEVKTMYEEWRVKHGKLNNNNIDESEKDKRFEIFKDNLKFIDEHNAENRTYKVGLNRFADLSNEEYRSRYLGTKIDPIGRTKTRSNRYAPSVGDKLPKSVDWRNQGAVVQVKDQGSCGSCWAFSTIAAVEGINKIVTGELVSLSEQELVDCDRTVNAGCDGGLMEYAFEFIINNGGIDSDEDYPYRGVDGKCDQYKKNARVVSIDDYEQVPAYDELALKKAVANQPISVAIEAGGREFQLYVSGIFTGKCGTALDHGVTVVGYGTENGVDYWIVRNSWGKSWGESGYVRMERNLATSVAGKCGIAMQSSYPIKKGQNPPNPGPSPPSPVNPPTVCSRYHSCASSTTCCCVLGIGKLCFSWGCCPLEAAVCCKDHSSCCPHDYPICNIHQGTCLRSKDNPFGVKAMERTPAKLHWPFGGQNEINIA