AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01357

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01357.t1 MTR_4g050760 96.605 324 11 0 1 324 1 324 0.0 635
MS.gene01357.t1 MTR_2g012380 96.605 324 11 0 1 324 1 324 0.0 635
MS.gene01357.t1 MTR_2g012420 92.593 324 24 0 1 324 1 324 0.0 608
MS.gene01357.t1 MTR_2g012380 96.538 260 9 0 1 260 1 260 0.0 510
MS.gene01357.t1 MTR_4g050710 93.852 244 15 0 81 324 4 247 3.12e-167 465
MS.gene01357.t1 MTR_2g012420 93.852 244 15 0 81 324 10 253 4.33e-167 465
MS.gene01357.t1 MTR_2g012420 95.312 192 9 0 133 324 1 192 1.75e-132 375
MS.gene01357.t1 MTR_4g029500 32.515 326 214 4 1 324 52 373 4.97e-41 147
MS.gene01357.t1 MTR_2g012390 87.302 63 8 0 82 144 5 67 6.41e-30 109
MS.gene01357.t1 MTR_8g064610 29.586 338 190 13 1 323 76 380 1.08e-25 105
MS.gene01357.t1 MTR_4g086400 31.818 220 140 5 26 239 175 390 2.24e-23 100
MS.gene01357.t1 MTR_3g093620 29.866 298 205 2 30 324 40 336 2.77e-23 99.0
MS.gene01357.t1 MTR_4g086400 31.818 220 140 5 26 239 315 530 3.29e-23 100
MS.gene01357.t1 MTR_4g086410 29.825 228 134 6 26 239 315 530 3.68e-21 94.7
MS.gene01357.t1 MTR_4g032995 27.628 333 201 12 10 323 16 327 6.12e-21 92.0
MS.gene01357.t1 MTR_4g036310 33.188 229 137 9 10 225 16 241 1.46e-20 90.9
MS.gene01357.t1 MTR_4g033205 27.327 333 202 12 10 323 16 327 1.25e-19 88.2
MS.gene01357.t1 MTR_7g055743 30.396 227 142 7 14 227 60 283 6.13e-19 86.7
MS.gene01357.t1 MTR_6g012170 28.182 330 181 13 13 323 24 316 1.15e-18 85.5
MS.gene01357.t1 MTR_8g035880 25.740 338 204 9 2 325 5 309 3.08e-17 81.6
MS.gene01357.t1 MTR_2g078520 25.797 345 220 9 2 323 27 358 3.34e-17 81.6
MS.gene01357.t1 MTR_8g035630 32.609 230 130 7 2 217 5 223 4.43e-17 81.3
MS.gene01357.t1 MTR_7g034395 29.129 333 174 13 13 323 21 313 1.20e-16 79.7
MS.gene01357.t1 MTR_6g012160 30.579 242 137 9 13 237 24 251 1.79e-14 73.2
MS.gene01357.t1 MTR_8g095220 23.739 337 193 10 1 278 54 385 4.65e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01357.t1 AT4G21580 73.375 323 86 0 1 323 1 323 1.31e-175 489
MS.gene01357.t1 AT4G21580 72.136 323 84 1 1 323 1 317 4.61e-170 475
MS.gene01357.t1 AT4G21580 71.379 290 83 0 34 323 1 290 5.46e-153 431
MS.gene01357.t1 AT5G61510 30.581 327 219 4 1 324 84 405 5.48e-37 137
MS.gene01357.t1 AT5G61510 29.936 314 212 4 14 324 40 348 2.23e-33 126
MS.gene01357.t1 AT1G23740 29.882 338 189 12 1 323 78 382 8.17e-27 109
MS.gene01357.t1 AT3G56460 27.545 334 217 7 7 325 15 338 2.13e-24 102
MS.gene01357.t1 AT1G49670 30.233 215 142 5 28 239 317 526 1.97e-20 92.4
MS.gene01357.t1 AT1G49670 29.237 236 140 7 28 239 317 549 8.05e-19 87.8
MS.gene01357.t1 AT3G15090 27.089 347 213 12 2 323 33 364 1.11e-18 86.3
MS.gene01357.t1 AT4G13010 27.914 326 209 9 10 323 16 327 1.12e-18 85.5
MS.gene01357.t1 AT5G63620 25.664 339 184 12 1 278 56 387 2.09e-15 77.0
MS.gene01357.t1 AT5G63620 25.369 339 185 12 1 278 56 387 3.96e-15 76.3

Find 89 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGATCTACGTGCTTTATT+TGG 0.204564 2.2:+69958703 MS.gene01357:CDS
ATGAGGACTTCATTGTCTTT+AGG 0.260405 2.2:-69957001 None:intergenic
CATGTTTCCAAGTGGAAAAT+TGG 0.267254 2.2:+69957151 MS.gene01357:CDS
TATGACGAGCCGGCTATCTA+AGG 0.294837 2.2:+69957878 MS.gene01357:CDS
ACCTTTCCTTCTGCAATTGC+AGG 0.305990 2.2:-69959739 None:intergenic
TTCGTTAATTCTCAGGTTCA+TGG 0.306876 2.2:+69958107 MS.gene01357:intron
GAACGGTATCGGCACTGTTA+AGG 0.335381 2.2:-69957042 None:intergenic
AATCATACGACTTACCAATA+AGG 0.346709 2.2:-69957907 None:intergenic
TGATGTTATTCTTGATTGTA+TGG 0.350274 2.2:+69958591 MS.gene01357:CDS
TCTTGAAGTTGTAGAACTTC+AGG 0.359966 2.2:-69956962 None:intergenic
AACGGTATCGGCACTGTTAA+GGG 0.374677 2.2:-69957041 None:intergenic
TATCCAGGTCTTGAATGTTC+TGG 0.375437 2.2:+69957106 MS.gene01357:CDS
AGAGAAATCCCGGTTGGAAC+TGG 0.378480 2.2:-69957801 None:intergenic
ATCAAGATTTCTTTGATAGT+AGG 0.380590 2.2:-69958620 None:intergenic
TGTGGAGGCTCACCGGCTTA+TGG 0.384620 2.2:+69959801 MS.gene01357:CDS
CAAGTGGAAAATTGGTGATC+AGG 0.404498 2.2:+69957159 MS.gene01357:CDS
CTTCATTGTCTTTAGGTTGA+GGG 0.404911 2.2:-69956994 None:intergenic
ACTTCATTGTCTTTAGGTTG+AGG 0.418079 2.2:-69956995 None:intergenic
GATAGGGGCTTGCACCTTGT+GGG 0.418781 2.2:-69957087 None:intergenic
CAGGTATGTGCTCTTCTAGC+TGG 0.420815 2.2:+69957732 MS.gene01357:intron
CAAGTTCTTCCAGTTCCAAC+CGG 0.428875 2.2:+69957792 MS.gene01357:CDS
GGGCTTGCACCTTGTGGGAT+AGG 0.434128 2.2:-69957082 None:intergenic
GCCTTTCCTTTGAACGGTAT+CGG 0.446179 2.2:-69957053 None:intergenic
TCAAAAGTATTTGTTACTGC+AGG 0.463368 2.2:+69958182 MS.gene01357:CDS
CTGAAGTGGAGAAGAATGTT+TGG 0.467969 2.2:+69959716 MS.gene01357:CDS
ATGGAAAGCAGTCAGCATAT+TGG 0.471076 2.2:+69959820 MS.gene01357:CDS
TTACAAAACAGAGGACTTTG+TGG 0.473910 2.2:+69958395 MS.gene01357:CDS
GGATAGGGGCTTGCACCTTG+TGG 0.476718 2.2:-69957088 None:intergenic
AGAACATTCAAGACCTGGAT+AGG 0.477853 2.2:-69957104 None:intergenic
GGCTTGCACCTTGTGGGATA+GGG 0.480797 2.2:-69957081 None:intergenic
GCAAGGGTAAAGGAAGAAAC+TGG 0.482737 2.2:+69958417 MS.gene01357:CDS
GGACTTTGTGGCAAGGGTAA+AGG 0.486871 2.2:+69958407 MS.gene01357:CDS
CCCTCTTTCCGAAGCTGTGG+AGG 0.490937 2.2:+69959786 MS.gene01357:CDS
CATGGAGGTTCAAGTGGAAT+CGG 0.496039 2.2:+69958125 MS.gene01357:CDS
ATCGGCACTGTTAAGGGCAG+TGG 0.498747 2.2:-69957035 None:intergenic
CCGGTGAGCCTCCACAGCTT+CGG 0.503841 2.2:-69959794 None:intergenic
GCCTCCACAGCTTCGGAAAG+AGG 0.508380 2.2:-69959787 None:intergenic
GTAAAAGCCTTTCCTTTGAA+CGG 0.516093 2.2:-69957059 None:intergenic
ATGACGAGCCGGCTATCTAA+GGG 0.518331 2.2:+69957879 MS.gene01357:CDS
GCCTGCAATTGCAGAAGGAA+AGG 0.519570 2.2:+69959738 MS.gene01357:CDS
GAACATTCAAGACCTGGATA+GGG 0.521615 2.2:-69957103 None:intergenic
CACTGTTAAGGGCAGTGGCA+TGG 0.523420 2.2:-69957030 None:intergenic
GGCAAGCGTCTCACTGTACA+AGG 0.526294 2.2:+69958724 MS.gene01357:CDS
TGAAGTTCTACAACTTCAAG+AGG 0.532066 2.2:+69956964 MS.gene01357:CDS
GATGTTATTCTTGATTGTAT+GGG 0.533185 2.2:+69958592 MS.gene01357:CDS
CCTCCACAGCTTCGGAAAGA+GGG 0.533582 2.2:-69959786 None:intergenic
CAGGTTCATGGAGGTTCAAG+TGG 0.534759 2.2:+69958119 MS.gene01357:intron
ACTGGAAGAACTTGTCCCTC+AGG 0.537643 2.2:-69957783 None:intergenic
CAAGGTGCAAGCCCCTATCC+AGG 0.537944 2.2:+69957091 MS.gene01357:CDS
TGCATCCTTCAGAGAAATCC+CGG 0.538209 2.2:-69957811 None:intergenic
TCCAACCGGGATTTCTCTGA+AGG 0.542035 2.2:+69957806 MS.gene01357:CDS
CCGAAGCTGTGGAGGCTCAC+CGG 0.543906 2.2:+69959794 MS.gene01357:CDS
GGGAATGATTTGTAGACCAC+AGG 0.553036 2.2:-69959766 None:intergenic
TCTGGAACTATTGAATCCGT+TGG 0.554523 2.2:+69957124 MS.gene01357:CDS
GCTGACTGCTTTCCATAAGC+CGG 0.555663 2.2:-69959813 None:intergenic
TGTGCTCTTCTAGCTGGAGG+TGG 0.560275 2.2:+69957738 MS.gene01357:CDS
CTTGGAAACATGTTTACCAA+CGG 0.565531 2.2:-69957140 None:intergenic
AAGGAAGAAACTGGCGGACA+AGG 0.566694 2.2:+69958426 MS.gene01357:CDS
GTTTGGCCTGCAATTGCAGA+AGG 0.566773 2.2:+69959733 MS.gene01357:CDS
GCCGATACCGTTCAAAGGAA+AGG 0.568367 2.2:+69957052 MS.gene01357:CDS
ATTCCCTCTTTCCGAAGCTG+TGG 0.571187 2.2:+69959783 MS.gene01357:CDS
AACAGAGGACTTTGTGGCAA+GGG 0.571801 2.2:+69958401 MS.gene01357:CDS
AAGTTCTTCCAGTTCCAACC+GGG 0.575692 2.2:+69957793 MS.gene01357:CDS
TCCTTCAGAGAAATCCCGGT+TGG 0.578830 2.2:-69957807 None:intergenic
TGGAAAGATATTGCTTGTTG+TGG 0.579900 2.2:+69959840 MS.gene01357:CDS
AGCTGTGATCGTTGCTGAAG+TGG 0.584468 2.2:+69959702 MS.gene01357:CDS
GTATGTGCTCTTCTAGCTGG+AGG 0.586099 2.2:+69957735 MS.gene01357:CDS
TGTAGAACTTCAGGTCCACC+TGG 0.592318 2.2:-69956953 None:intergenic
AACATTCAAGACCTGGATAG+GGG 0.603099 2.2:-69957102 None:intergenic
AAGAGTATTGGAGCTGATGT+TGG 0.603468 2.2:+69958366 MS.gene01357:CDS
GTTCCAGAACATTCAAGACC+TGG 0.611446 2.2:-69957109 None:intergenic
CAGTGGCATGGACTCTGATG+AGG 0.615749 2.2:-69957018 None:intergenic
ACAGTGCCGATACCGTTCAA+AGG 0.616788 2.2:+69957047 MS.gene01357:CDS
ATATTTCTTAGCAGGTAGTG+AGG 0.629845 2.2:+69958326 MS.gene01357:intron
AGAAGGAAAGGTTACGCCTG+TGG 0.632772 2.2:+69959750 MS.gene01357:CDS
TTTATTATCGGCTTTCAAGG+GGG 0.643203 2.2:+69958667 MS.gene01357:CDS
TTGGTAAACATGTTTCCAAG+TGG 0.643296 2.2:+69957143 MS.gene01357:CDS
AAACAGAGGACTTTGTGGCA+AGG 0.650379 2.2:+69958400 MS.gene01357:CDS
GACGAGCCGGCTATCTAAGG+GGG 0.651572 2.2:+69957881 MS.gene01357:CDS
TGAGAAAGTAGCAGTTCCTG+AGG 0.656971 2.2:+69957767 MS.gene01357:CDS
GTTAATTCTCAGGTTCATGG+AGG 0.666886 2.2:+69958110 MS.gene01357:intron
GAGAAAGTAGCAGTTCCTGA+GGG 0.670291 2.2:+69957768 MS.gene01357:CDS
GCTATTGTAATAACAACACC+AGG 0.682237 2.2:+69956935 MS.gene01357:CDS
TGACGAGCCGGCTATCTAAG+GGG 0.683970 2.2:+69957880 MS.gene01357:CDS
CTGAGGTTGCATGTACTGTG+TGG 0.713501 2.2:+69957844 MS.gene01357:CDS
TGGTATCAATTACAAAACAG+AGG 0.714141 2.2:+69958386 MS.gene01357:CDS
ATTGTAATAACAACACCAGG+TGG 0.715936 2.2:+69956938 MS.gene01357:CDS
ATTCAGATAGCTAAGTACCG+AGG 0.723680 2.2:+69958158 MS.gene01357:CDS
AGGGTAAAGGAAGAAACTGG+CGG 0.742355 2.2:+69958420 MS.gene01357:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAACAAGATATTAAT+TGG - chr2.2:69958517-69958536 None:intergenic 10.0%
!!! TTAATATCTTGTTTTTTAAT+TGG + chr2.2:69958518-69958537 MS.gene01357:intron 10.0%
!! TATATCAATAATTGAATTGT+GGG + chr2.2:69957226-69957245 MS.gene01357:intron 15.0%
!! TATGAAAAAGAAAGTAAAAA+CGG + chr2.2:69957550-69957569 MS.gene01357:intron 15.0%
!! TTATATCAATAATTGAATTG+TGG + chr2.2:69957225-69957244 MS.gene01357:intron 15.0%
!! ATATAGATCATATAATGTTG+AGG - chr2.2:69958490-69958509 None:intergenic 20.0%
!! ATCGAATATTAAAGAATGAT+AGG - chr2.2:69959632-69959651 None:intergenic 20.0%
!! ATTCTTTAATATTCGATTGT+GGG + chr2.2:69959635-69959654 MS.gene01357:intron 20.0%
!! TACTTAATACATCAACTATT+AGG - chr2.2:69958082-69958101 None:intergenic 20.0%
!! TGAATGGATATTATAATATG+AGG + chr2.2:69957697-69957716 MS.gene01357:intron 20.0%
!! TGAGTATCTAATATACAAAA+GGG - chr2.2:69958770-69958789 None:intergenic 20.0%
!!! AGTTCTAGTTATTATATAAC+TGG - chr2.2:69957275-69957294 None:intergenic 20.0%
!!! ATTCATATTTTTCTCCAATT+GGG + chr2.2:69957196-69957215 MS.gene01357:intron 20.0%
!!! TTTTGAATTTCATCTTTATG+AGG + chr2.2:69959535-69959554 MS.gene01357:intron 20.0%
! ACCAAATAATAAAATAGACC+AGG - chr2.2:69958048-69958067 None:intergenic 25.0%
! AGGTAATATTATCATTGATG+TGG + chr2.2:69958558-69958577 MS.gene01357:intron 25.0%
! AGTTTATGAAATTTCACCTA+AGG + chr2.2:69958939-69958958 MS.gene01357:intron 25.0%
! ATATTATAATATGAGGTGTG+TGG + chr2.2:69957704-69957723 MS.gene01357:intron 25.0%
! ATGTTGCAAAATATCTACTA+AGG - chr2.2:69959196-69959215 None:intergenic 25.0%
! ATTCACATTATGCTTCATAT+AGG - chr2.2:69958457-69958476 None:intergenic 25.0%
! ATTGTGAGATTCATTATTGT+AGG - chr2.2:69959125-69959144 None:intergenic 25.0%
! CAGTCTTTATTACTAGAATA+AGG + chr2.2:69957424-69957443 MS.gene01357:intron 25.0%
! CATTCTTTAATATTCGATTG+TGG + chr2.2:69959634-69959653 MS.gene01357:intron 25.0%
! CTAATATGACCAACTTTAAT+CGG - chr2.2:69959300-69959319 None:intergenic 25.0%
! GATGTTATTCTTGATTGTAT+GGG + chr2.2:69958592-69958611 MS.gene01357:CDS 25.0%
! GTGAGTATCTAATATACAAA+AGG - chr2.2:69958771-69958790 None:intergenic 25.0%
! TAATATTATCATTGATGTGG+TGG + chr2.2:69958561-69958580 MS.gene01357:intron 25.0%
! TATTAACTTCGTTAATTCTC+AGG + chr2.2:69958100-69958119 MS.gene01357:intron 25.0%
! TGATATAAAGAAAACCCAAT+TGG - chr2.2:69957213-69957232 None:intergenic 25.0%
! TGATGTTATTCTTGATTGTA+TGG + chr2.2:69958591-69958610 MS.gene01357:CDS 25.0%
!! ACTGTTTTCATAATCTATCT+TGG + chr2.2:69957521-69957540 MS.gene01357:intron 25.0%
!! ATCAAGATTTCTTTGATAGT+AGG - chr2.2:69958623-69958642 None:intergenic 25.0%
!! CATTCATATTTTTCTCCAAT+TGG + chr2.2:69957195-69957214 MS.gene01357:intron 25.0%
!! TAACAAATACTTTTGATCCT+CGG - chr2.2:69958178-69958197 None:intergenic 25.0%
!! TAGTAGATATTTTGCAACAT+TGG + chr2.2:69959196-69959215 MS.gene01357:intron 25.0%
!! TCCTGGTCTATTTTATTATT+TGG + chr2.2:69958044-69958063 MS.gene01357:intron 25.0%
!! TCTTTTTATTAGTGTGGTTA+TGG + chr2.2:69959600-69959619 MS.gene01357:intron 25.0%
!!! ATAGCTTAAATTTTGATGGA+AGG + chr2.2:69958641-69958660 MS.gene01357:CDS 25.0%
!!! ATTGTAGGCTTTTTTTTTCA+TGG - chr2.2:69959110-69959129 None:intergenic 25.0%
!!! CTTGATAGCTTAAATTTTGA+TGG + chr2.2:69958637-69958656 MS.gene01357:CDS 25.0%
!!! GTATTGATGATGACTATATA+AGG + chr2.2:69959145-69959164 MS.gene01357:intron 25.0%
!!! TTTTTATTATCGGCTTTCAA+GGG + chr2.2:69958665-69958684 MS.gene01357:CDS 25.0%
AAAAATGATGTGTCTACAGA+AGG - chr2.2:69959505-69959524 None:intergenic 30.0%
AAATATCTGCAAACATCACT+TGG - chr2.2:69958298-69958317 None:intergenic 30.0%
AAATTGCTAATGTAAGTGAC+TGG - chr2.2:69958905-69958924 None:intergenic 30.0%
AACGTGATTGGTATGTATAA+AGG - chr2.2:69959475-69959494 None:intergenic 30.0%
AATCATACGACTTACCAATA+AGG - chr2.2:69957910-69957929 None:intergenic 30.0%
AATTGAGTCAAATCAGTTAG+AGG + chr2.2:69959432-69959451 MS.gene01357:intron 30.0%
ACATCTAAACATACGAAAAC+TGG - chr2.2:69958236-69958255 None:intergenic 30.0%
ATATAATGTTGAGGAATGCT+TGG - chr2.2:69958481-69958500 None:intergenic 30.0%
ATGAAATTTCACCTAAGGTT+GGG + chr2.2:69958944-69958963 MS.gene01357:intron 30.0%
ATTGAGTCAAATCAGTTAGA+GGG + chr2.2:69959433-69959452 MS.gene01357:intron 30.0%
CATGAAAGTTGTCATTCTAT+AGG - chr2.2:69959372-69959391 None:intergenic 30.0%
CTTTCATGAACTGTTATACT+CGG + chr2.2:69959384-69959403 MS.gene01357:intron 30.0%
GAGTAGCTAATTCAAATCAA+AGG - chr2.2:69957360-69957379 None:intergenic 30.0%
TACTAGAATAAGGTTCTGTT+TGG + chr2.2:69957434-69957453 MS.gene01357:intron 30.0%
TATGAAATTTCACCTAAGGT+TGG + chr2.2:69958943-69958962 MS.gene01357:intron 30.0%
TATTCCAGATATTTCTTAGC+AGG + chr2.2:69958318-69958337 MS.gene01357:intron 30.0%
TGCTTGTACAAGTAAAAACA+CGG + chr2.2:69958834-69958853 MS.gene01357:intron 30.0%
TGGTATCAATTACAAAACAG+AGG + chr2.2:69958386-69958405 MS.gene01357:CDS 30.0%
TTAAGTGATGATAAGTGCTT+TGG + chr2.2:69957470-69957489 MS.gene01357:intron 30.0%
TTGCAAAATATCTACTAAGG+CGG - chr2.2:69959193-69959212 None:intergenic 30.0%
! AGCTTTTCCACAATTGAAAT+GGG + chr2.2:69959573-69959592 MS.gene01357:intron 30.0%
! CATTAACTACCGATTAAAGT+TGG + chr2.2:69959288-69959307 MS.gene01357:intron 30.0%
! GATGGAAGGCTTTTTATTAT+CGG + chr2.2:69958655-69958674 MS.gene01357:CDS 30.0%
! GGAAGTTCTTTTTATTAGTG+TGG + chr2.2:69959594-69959613 MS.gene01357:intron 30.0%
! TAATCAACTGACAACTTTTC+TGG + chr2.2:69957931-69957950 MS.gene01357:intron 30.0%
!! TCAAAAGTATTTGTTACTGC+AGG + chr2.2:69958182-69958201 MS.gene01357:CDS 30.0%
!! TCTGTAGACACATCATTTTT+AGG + chr2.2:69959505-69959524 MS.gene01357:intron 30.0%
!!! AACGATCACAGCTTTATTTT+CGG - chr2.2:69959695-69959714 None:intergenic 30.0%
!!! CTTTTTATTATCGGCTTTCA+AGG + chr2.2:69958664-69958683 MS.gene01357:CDS 30.0%
!!! TTTTATTATCGGCTTTCAAG+GGG + chr2.2:69958666-69958685 MS.gene01357:CDS 30.0%
ACTACCTGCTAAGAAATATC+TGG - chr2.2:69958325-69958344 None:intergenic 35.0%
AGAACTTCCCATTTCAATTG+TGG - chr2.2:69959583-69959602 None:intergenic 35.0%
ATACATACCAATCACGTTTG+TGG + chr2.2:69959477-69959496 MS.gene01357:intron 35.0%
ATATTTCTTAGCAGGTAGTG+AGG + chr2.2:69958326-69958345 MS.gene01357:intron 35.0%
ATGAGGACTTCATTGTCTTT+AGG - chr2.2:69957004-69957023 None:intergenic 35.0%
ATTGTAATAACAACACCAGG+TGG + chr2.2:69956938-69956957 MS.gene01357:CDS 35.0%
CATGTTTCCAAGTGGAAAAT+TGG + chr2.2:69957151-69957170 MS.gene01357:CDS 35.0%
CATTTCAATTGTGGAAAAGC+TGG - chr2.2:69959574-69959593 None:intergenic 35.0%
CTTGGAAACATGTTTACCAA+CGG - chr2.2:69957143-69957162 None:intergenic 35.0%
GCAGAAAACTAAACTTGAAG+AGG - chr2.2:69958803-69958822 None:intergenic 35.0%
GCTATTGTAATAACAACACC+AGG + chr2.2:69956935-69956954 MS.gene01357:CDS 35.0%
GGCTTCAATAACAAGAAAAG+CGG - chr2.2:69958215-69958234 None:intergenic 35.0%
GTAAAAGCCTTTCCTTTGAA+CGG - chr2.2:69957062-69957081 None:intergenic 35.0%
TCACATAGGGTTGTTTAGAT+TGG - chr2.2:69957658-69957677 None:intergenic 35.0%
TCACTGTATAACTATCTTCC+TGG + chr2.2:69958027-69958046 MS.gene01357:intron 35.0%
TCTTGAAGTTGTAGAACTTC+AGG - chr2.2:69956965-69956984 None:intergenic 35.0%
TCTTTGCAAGCATGGTTATT+GGG + chr2.2:69957970-69957989 MS.gene01357:intron 35.0%
TGAAGTTCTACAACTTCAAG+AGG + chr2.2:69956964-69956983 MS.gene01357:CDS 35.0%
TGTACAAGGTAATGCAGATT+CGG + chr2.2:69958738-69958757 MS.gene01357:intron 35.0%
TGTTGCGTTGTGATTATGAA+TGG + chr2.2:69957681-69957700 MS.gene01357:intron 35.0%
TTACAAAACAGAGGACTTTG+TGG + chr2.2:69958395-69958414 MS.gene01357:CDS 35.0%
TTCGTTAATTCTCAGGTTCA+TGG + chr2.2:69958107-69958126 MS.gene01357:intron 35.0%
TTGGTAAACATGTTTCCAAG+TGG + chr2.2:69957143-69957162 MS.gene01357:CDS 35.0%
! CAGCTTTTCCACAATTGAAA+TGG + chr2.2:69959572-69959591 MS.gene01357:intron 35.0%
! GTAGATCTACGTGCTTTATT+TGG + chr2.2:69958703-69958722 MS.gene01357:CDS 35.0%
! TGGAAAGATATTGCTTGTTG+TGG + chr2.2:69959840-69959859 MS.gene01357:CDS 35.0%
! TTTTCATAAGCTCTTCCGAA+CGG + chr2.2:69957595-69957614 MS.gene01357:intron 35.0%
!! ACTTCATTGTCTTTAGGTTG+AGG - chr2.2:69956998-69957017 None:intergenic 35.0%
!! CTAGCTTTTTGCAAGAGTAT+TGG + chr2.2:69958354-69958373 MS.gene01357:CDS 35.0%
!! CTTCATTGTCTTTAGGTTGA+GGG - chr2.2:69956997-69957016 None:intergenic 35.0%
!! TTTATTATCGGCTTTCAAGG+GGG + chr2.2:69958667-69958686 MS.gene01357:CDS 35.0%
!!! ACGATCACAGCTTTATTTTC+GGG - chr2.2:69959694-69959713 None:intergenic 35.0%
AACATTCAAGACCTGGATAG+GGG - chr2.2:69957105-69957124 None:intergenic 40.0%
AGAACATTCAAGACCTGGAT+AGG - chr2.2:69957107-69957126 None:intergenic 40.0%
ATCTAAGGGGGAAACCTTAT+TGG + chr2.2:69957893-69957912 MS.gene01357:CDS 40.0%
ATGGAAAGCAGTCAGCATAT+TGG + chr2.2:69959820-69959839 MS.gene01357:CDS 40.0%
ATTATCATTGATGTGGTGGC+AGG + chr2.2:69958565-69958584 MS.gene01357:intron 40.0%
CTCTTTGCAAGCATGGTTAT+TGG + chr2.2:69957969-69957988 MS.gene01357:intron 40.0%
CTGAAGTGGAGAAGAATGTT+TGG + chr2.2:69959716-69959735 MS.gene01357:CDS 40.0%
GAACATTCAAGACCTGGATA+GGG - chr2.2:69957106-69957125 None:intergenic 40.0%
GAAGACGCTTCAATGCAAAA+TGG - chr2.2:69959051-69959070 None:intergenic 40.0%
GTTAATTCTCAGGTTCATGG+AGG + chr2.2:69958110-69958129 MS.gene01357:intron 40.0%
TAGCATACTCTTTGCAAGCA+TGG + chr2.2:69957962-69957981 MS.gene01357:intron 40.0%
TATCCAGGTCTTGAATGTTC+TGG + chr2.2:69957106-69957125 MS.gene01357:CDS 40.0%
TCTACTAAGGCGGACAAAAA+TGG - chr2.2:69959183-69959202 None:intergenic 40.0%
TGGTTGCTTAGAGTTGTGTT+AGG + chr2.2:69958538-69958557 MS.gene01357:intron 40.0%
TTCATTTGACAGCACACTAC+AGG - chr2.2:69959261-69959280 None:intergenic 40.0%
! ATTCAGATAGCTAAGTACCG+AGG + chr2.2:69958158-69958177 MS.gene01357:CDS 40.0%
! CAAGTGGAAAATTGGTGATC+AGG + chr2.2:69957159-69957178 MS.gene01357:CDS 40.0%
! CTGATCACCAATTTTCCACT+TGG - chr2.2:69957161-69957180 None:intergenic 40.0%
! TCTGGAACTATTGAATCCGT+TGG + chr2.2:69957124-69957143 MS.gene01357:CDS 40.0%
!! AAGAGTATTGGAGCTGATGT+TGG + chr2.2:69958366-69958385 MS.gene01357:CDS 40.0%
!! TTTTGCATTGAAGCGTCTTC+TGG + chr2.2:69959051-69959070 MS.gene01357:intron 40.0%
!!! CGACTATTTTTATGACGAGC+CGG + chr2.2:69957868-69957887 MS.gene01357:CDS 40.0%
AAACAGAGGACTTTGTGGCA+AGG + chr2.2:69958400-69958419 MS.gene01357:CDS 45.0%
AACAGAGGACTTTGTGGCAA+GGG + chr2.2:69958401-69958420 MS.gene01357:CDS 45.0%
AAGTTCTTCCAGTTCCAACC+GGG + chr2.2:69957793-69957812 MS.gene01357:CDS 45.0%
ACAACGCAACACACTCACAT+AGG - chr2.2:69957672-69957691 None:intergenic 45.0%
ACCTTTCCTTCTGCAATTGC+AGG - chr2.2:69959742-69959761 None:intergenic 45.0%
AGGGTAAAGGAAGAAACTGG+CGG + chr2.2:69958420-69958439 MS.gene01357:CDS 45.0%
CAACGCAACACACTCACATA+GGG - chr2.2:69957671-69957690 None:intergenic 45.0%
CAAGTAAAAACACGGCTCGA+CGG + chr2.2:69958842-69958861 MS.gene01357:intron 45.0%
CAAGTTCTTCCAGTTCCAAC+CGG + chr2.2:69957792-69957811 MS.gene01357:CDS 45.0%
CATGGAGGTTCAAGTGGAAT+CGG + chr2.2:69958125-69958144 MS.gene01357:CDS 45.0%
GAGAAAGTAGCAGTTCCTGA+GGG + chr2.2:69957768-69957787 MS.gene01357:CDS 45.0%
GCAAGGGTAAAGGAAGAAAC+TGG + chr2.2:69958417-69958436 MS.gene01357:CDS 45.0%
GCCTTTCCTTTGAACGGTAT+CGG - chr2.2:69957056-69957075 None:intergenic 45.0%
GTCCACAATAGCTTACTGTG+TGG + chr2.2:69959344-69959363 MS.gene01357:intron 45.0%
GTTCCAGAACATTCAAGACC+TGG - chr2.2:69957112-69957131 None:intergenic 45.0%
TGAGAAAGTAGCAGTTCCTG+AGG + chr2.2:69957767-69957786 MS.gene01357:CDS 45.0%
TGCATCCTTCAGAGAAATCC+CGG - chr2.2:69957814-69957833 None:intergenic 45.0%
TGCCACACAGTAAGCTATTG+TGG - chr2.2:69959349-69959368 None:intergenic 45.0%
! CGATTGTGGGTTATGTTCAG+CGG + chr2.2:69959648-69959667 MS.gene01357:intron 45.0%
! GGGAATGATTTGTAGACCAC+AGG - chr2.2:69959769-69959788 None:intergenic 45.0%
!! AACGGTATCGGCACTGTTAA+GGG - chr2.2:69957044-69957063 None:intergenic 45.0%
!! TAAGCACTTCTGAGACCGTT+CGG - chr2.2:69957613-69957632 None:intergenic 45.0%
!!! GGATGCAGCTAGTTTTACTG+AGG + chr2.2:69957827-69957846 MS.gene01357:CDS 45.0%
ACTGGAAGAACTTGTCCCTC+AGG - chr2.2:69957786-69957805 None:intergenic 50.0%
AGAAGGAAAGGTTACGCCTG+TGG + chr2.2:69959750-69959769 MS.gene01357:CDS 50.0%
AGAGAAATCCCGGTTGGAAC+TGG - chr2.2:69957804-69957823 None:intergenic 50.0%
AGCTGTGATCGTTGCTGAAG+TGG + chr2.2:69959702-69959721 MS.gene01357:CDS 50.0%
AGGTTTCCCCCTTAGATAGC+CGG - chr2.2:69957890-69957909 None:intergenic 50.0%
ATGACGAGCCGGCTATCTAA+GGG + chr2.2:69957879-69957898 MS.gene01357:CDS 50.0%
ATTCCCTCTTTCCGAAGCTG+TGG + chr2.2:69959783-69959802 MS.gene01357:CDS 50.0%
ATTTCACCTAAGGTTGGGCG+CGG + chr2.2:69958949-69958968 MS.gene01357:intron 50.0%
CAGGTATGTGCTCTTCTAGC+TGG + chr2.2:69957732-69957751 MS.gene01357:intron 50.0%
CAGGTTCATGGAGGTTCAAG+TGG + chr2.2:69958119-69958138 MS.gene01357:intron 50.0%
CTGAGGTTGCATGTACTGTG+TGG + chr2.2:69957844-69957863 MS.gene01357:CDS 50.0%
GACAGCACACTACAGGAACA+AGG - chr2.2:69959254-69959273 None:intergenic 50.0%
GCCGATACCGTTCAAAGGAA+AGG + chr2.2:69957052-69957071 MS.gene01357:CDS 50.0%
GCCTGCAATTGCAGAAGGAA+AGG + chr2.2:69959738-69959757 MS.gene01357:CDS 50.0%
GCTGACTGCTTTCCATAAGC+CGG - chr2.2:69959816-69959835 None:intergenic 50.0%
GGACTTTGTGGCAAGGGTAA+AGG + chr2.2:69958407-69958426 MS.gene01357:CDS 50.0%
GTTTGGCCTGCAATTGCAGA+AGG + chr2.2:69959733-69959752 MS.gene01357:CDS 50.0%
TATGACGAGCCGGCTATCTA+AGG + chr2.2:69957878-69957897 MS.gene01357:CDS 50.0%
TATGAGGTGTGTGGCTTCAC+AGG + chr2.2:69957713-69957732 MS.gene01357:intron 50.0%
TCCAACCGGGATTTCTCTGA+AGG + chr2.2:69957806-69957825 MS.gene01357:CDS 50.0%
TCCTTCAGAGAAATCCCGGT+TGG - chr2.2:69957810-69957829 None:intergenic 50.0%
TGTAGAACTTCAGGTCCACC+TGG - chr2.2:69956956-69956975 None:intergenic 50.0%
! AAGGAAGAAACTGGCGGACA+AGG + chr2.2:69958426-69958445 MS.gene01357:CDS 50.0%
! GGCTTTTACCCTATCCCACA+AGG + chr2.2:69957073-69957092 MS.gene01357:CDS 50.0%
! GTATGTGCTCTTCTAGCTGG+AGG + chr2.2:69957735-69957754 MS.gene01357:CDS 50.0%
!! ACAGTGCCGATACCGTTCAA+AGG + chr2.2:69957047-69957066 MS.gene01357:CDS 50.0%
!! GAACGGTATCGGCACTGTTA+AGG - chr2.2:69957045-69957064 None:intergenic 50.0%
!! GAAGGTGCCACAAACGTGAT+TGG - chr2.2:69959487-69959506 None:intergenic 50.0%
ATCGGCACTGTTAAGGGCAG+TGG - chr2.2:69957038-69957057 None:intergenic 55.0%
CACTGTTAAGGGCAGTGGCA+TGG - chr2.2:69957033-69957052 None:intergenic 55.0%
CAGTGGCATGGACTCTGATG+AGG - chr2.2:69957021-69957040 None:intergenic 55.0%
CCTCCACAGCTTCGGAAAGA+GGG - chr2.2:69959789-69959808 None:intergenic 55.0%
GATAGGGGCTTGCACCTTGT+GGG - chr2.2:69957090-69957109 None:intergenic 55.0%
GGCAAGCGTCTCACTGTACA+AGG + chr2.2:69958724-69958743 MS.gene01357:CDS 55.0%
GGCTTGCACCTTGTGGGATA+GGG - chr2.2:69957084-69957103 None:intergenic 55.0%
TGACGAGCCGGCTATCTAAG+GGG + chr2.2:69957880-69957899 MS.gene01357:CDS 55.0%
! TGTGCTCTTCTAGCTGGAGG+TGG + chr2.2:69957738-69957757 MS.gene01357:CDS 55.0%
! TGTGGGTTATGTTCAGCGGC+TGG + chr2.2:69959652-69959671 MS.gene01357:intron 55.0%
CAAGGTGCAAGCCCCTATCC+AGG + chr2.2:69957091-69957110 MS.gene01357:CDS 60.0%
CCCTCTTTCCGAAGCTGTGG+AGG + chr2.2:69959786-69959805 MS.gene01357:CDS 60.0%
GACGAGCCGGCTATCTAAGG+GGG + chr2.2:69957881-69957900 MS.gene01357:CDS 60.0%
GCCTCCACAGCTTCGGAAAG+AGG - chr2.2:69959790-69959809 None:intergenic 60.0%
GGATAGGGGCTTGCACCTTG+TGG - chr2.2:69957091-69957110 None:intergenic 60.0%
GGGCTTGCACCTTGTGGGAT+AGG - chr2.2:69957085-69957104 None:intergenic 60.0%
!! TGTGGAGGCTCACCGGCTTA+TGG + chr2.2:69959801-69959820 MS.gene01357:CDS 60.0%
ACCTAAGGTTGGGCGCGGAG+AGG + chr2.2:69958954-69958973 MS.gene01357:intron 65.0%
CCGAAGCTGTGGAGGCTCAC+CGG + chr2.2:69959794-69959813 MS.gene01357:CDS 65.0%
CCGGTGAGCCTCCACAGCTT+CGG - chr2.2:69959797-69959816 None:intergenic 65.0%
TCCTCTCCGCGCCCAACCTT+AGG - chr2.2:69958958-69958977 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 69956929 69959867 69956929 ID=MS.gene01357
chr2.2 mRNA 69956929 69959867 69956929 ID=MS.gene01357.t1;Parent=MS.gene01357
chr2.2 exon 69956929 69957180 69956929 ID=MS.gene01357.t1.exon1;Parent=MS.gene01357.t1
chr2.2 CDS 69956929 69957180 69956929 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
chr2.2 exon 69957735 69957914 69957735 ID=MS.gene01357.t1.exon2;Parent=MS.gene01357.t1
chr2.2 CDS 69957735 69957914 69957735 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
chr2.2 exon 69958122 69958203 69958122 ID=MS.gene01357.t1.exon3;Parent=MS.gene01357.t1
chr2.2 CDS 69958122 69958203 69958122 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
chr2.2 exon 69958340 69958447 69958340 ID=MS.gene01357.t1.exon4;Parent=MS.gene01357.t1
chr2.2 CDS 69958340 69958447 69958340 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
chr2.2 exon 69958587 69958745 69958587 ID=MS.gene01357.t1.exon5;Parent=MS.gene01357.t1
chr2.2 CDS 69958587 69958745 69958587 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
chr2.2 exon 69959668 69959867 69959668 ID=MS.gene01357.t1.exon6;Parent=MS.gene01357.t1
chr2.2 CDS 69959668 69959867 69959668 ID=cds.MS.gene01357.t1;Parent=MS.gene01357.t1
Gene Sequence

>MS.gene01357

ATGAAGGCTATTGTAATAACAACACCAGGTGGACCTGAAGTTCTACAACTTCAAGAGGTTCAAGACCCTCAACCTAAAGACAATGAAGTCCTCATCAGAGTCCATGCCACTGCCCTTAACAGTGCCGATACCGTTCAAAGGAAAGGCTTTTACCCTATCCCACAAGGTGCAAGCCCCTATCCAGGTCTTGAATGTTCTGGAACTATTGAATCCGTTGGTAAACATGTTTCCAAGTGGAAAATTGGTGATCAGGTATACAGCTTTTTCATTCATATTTTTCTCCAATTGGGTTTTCTTTATATCAATAATTGAATTGTGGGTCTATAAAAAGTTTTGAGCTTTTCCAGTTATATAATAACTAGAACTTCATATATATATATATATATATATATATATATATATATATATATATATATATATAGCATATACCTTTGATTTGAATTAGCTACTCAACTTGATTATTAAACATTAGAACAATTACAATCTCTAAAATTTCAGTCTTTATTACTAGAATAAGGTTCTGTTTGGATAAACAACTTGATTAAGTGATGATAAGTGCTTTGGTATGAGCTATTTTTATAAAATACATTAAACTGTTTTCATAATCTATCTTGGAGAGCTTATGAAAAAGAAAGTAAAAACGGCTTCTTGACATATCATAAGCTGTTTTCATAAGCTCTTCCGAACGGTCTCAGAAGTGCTTATGTCAGTAGACAACTTAAAAAGCCAATCTAAACAACCCTATGTGAGTGTGTTGCGTTGTGATTATGAATGGATATTATAATATGAGGTGTGTGGCTTCACAGGTATGTGCTCTTCTAGCTGGAGGTGGTTATGCTGAGAAAGTAGCAGTTCCTGAGGGACAAGTTCTTCCAGTTCCAACCGGGATTTCTCTGAAGGATGCAGCTAGTTTTACTGAGGTTGCATGTACTGTGTGGTCGACTATTTTTATGACGAGCCGGCTATCTAAGGGGGAAACCTTATTGGTAAGTCGTATGATTGTAATCAACTGACAACTTTTCTGGAAAATACATAGCATACTCTTTGCAAGCATGGTTATTGGGCATTTTGTCACTGTTTCTTGACATGCTTTTGATCTCACTGTATAACTATCTTCCTGGTCTATTTTATTATTTGGTAGTAAGTGTAGCCTAATAGTTGATGTATTAAGTATTAACTTCGTTAATTCTCAGGTTCATGGAGGTTCAAGTGGAATCGGAACATTTGCAATTCAGATAGCTAAGTACCGAGGATCAAAAGTATTTGTTACTGCAGGTTCAGTGCCGCTTTTCTTGTTATTGAAGCCAGTTTTCGTATGTTTAGATGTGAAACTGATGCTATTTCAAGTTTCTCGCATCTTGAAATTCCAAGTGATGTTTGCAGATATTTTATTCCAGATATTTCTTAGCAGGTAGTGAGGCGAAGCTAGCTTTTTGCAAGAGTATTGGAGCTGATGTTGGTATCAATTACAAAACAGAGGACTTTGTGGCAAGGGTAAAGGAAGAAACTGGCGGACAAGGTATTACCTATATGAAGCATAATGTGAATTCCAAGCATTCCTCAACATTATATGATCTATATTGTACCAATTAATATCTTGTTTTTTAATTGGTTGCTTAGAGTTGTGTTAGGTAATATTATCATTGATGTGGTGGCAGGTGTTGATGTTATTCTTGATTGTATGGGAGCATCCTACTATCAAAGAAATCTTGATAGCTTAAATTTTGATGGAAGGCTTTTTATTATCGGCTTTCAAGGGGGTGTTTCTACAGATGTAGATCTACGTGCTTTATTTGGCAAGCGTCTCACTGTACAAGGTAATGCAGATTCGGATTATACCCTTTTGTATATTAGATACTCACTCAGTCACTCCTCTTCAAGTTTAGTTTTCTGCATGTAAGTATTTGCTTGTACAAGTAAAAACACGGCTCGACGGTCTATATATTAGAAGATGAAGAAATAAAAGTAAATAGCCAGTCACTTACATTAGCAATTTAAACAATACAACATAGTTTATGAAATTTCACCTAAGGTTGGGCGCGGAGAGGAATGAAACTGCTTAAAATAGTCGACTGACTTCTTACAAATTTTGTTTTGTATACTCGTGATATATCTTTCTCCATTTTGCATTGAAGCGTCTTCTGGTTTTTTGTGCACATTTTAATATCACTTATAGCTCCATGAAAAAAAAAGCCTACAATAATGAATCTCACAATGTATTGATGATGACTATATAAGGCAGCTTGAGTAACCATTTTTGTCCGCCTTAGTAGATATTTTGCAACATTGGCTGCTACGCATAAATAAAATTCTTGTAGTAAGCCTTGTTCCTGTAGTGTGCTGTCAAATGAAAACAATACATTAACTACCGATTAAAGTTGGTCATATTAGAATATAGCGTATAAGCTCACTACAGTCCACAATAGCTTACTGTGTGGCACCTATAGAATGACAACTTTCATGAACTGTTATACTCGGTGTAACTAACTAGAGATCTCTAACTAATTGAGTCAAATCAGTTAGAGGGATCAAGAATTCTTTGACCTTTATACATACCAATCACGTTTGTGGCACCTTCTGTAGACACATCATTTTTAGGCATCTGCTTTTGAATTTCATCTTTATGAGGATACATTAGAGAACCAGCTTTTCCACAATTGAAATGGGAAGTTCTTTTTATTAGTGTGGTTATGGTATGTACCTATCATTCTTTAATATTCGATTGTGGGTTATGTTCAGCGGCTGGCTTGCGAAGTAGAAGTCCCGAAAATAAAGCTGTGATCGTTGCTGAAGTGGAGAAGAATGTTTGGCCTGCAATTGCAGAAGGAAAGGTTACGCCTGTGGTCTACAAATCATTCCCTCTTTCCGAAGCTGTGGAGGCTCACCGGCTTATGGAAAGCAGTCAGCATATTGGAAAGATATTGCTTGTTGTGGCATGA
Protein sequence

>MS.gene01357.t1

MKAIVITTPGGPEVLQLQEVQDPQPKDNEVLIRVHATALNSADTVQRKGFYPIPQGASPYPGLECSGTIESVGKHVSKWKIGDQVCALLAGGGYAEKVAVPEGQVLPVPTGISLKDAASFTEVACTVWSTIFMTSRLSKGETLLVHGGSSGIGTFAIQIAKYRGSKVFVTAGSEAKLAFCKSIGADVGINYKTEDFVARVKEETGGQGVDVILDCMGASYYQRNLDSLNFDGRLFIIGFQGGVSTDVDLRALFGKRLTVQAAGLRSRSPENKAVIVAEVEKNVWPAIAEGKVTPVVYKSFPLSEAVEAHRLMESSQHIGKILLVVA