AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01364

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01364.t1 MTR_2g012510 98.241 398 7 0 1 398 69 466 0.0 812
MS.gene01364.t1 MTR_4g090525 70.927 399 102 3 1 398 65 450 0.0 561
MS.gene01364.t1 MTR_8g464260 67.725 378 119 1 21 398 4 378 0.0 530
MS.gene01364.t1 MTR_4g094672 40.070 287 135 6 79 356 1 259 3.34e-61 199
MS.gene01364.t1 MTR_2g011680 33.588 393 209 16 18 389 44 405 8.17e-41 149
MS.gene01364.t1 MTR_3g109970 76.667 60 13 1 75 133 6 65 2.80e-23 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01364.t1 AT5G54390 64.075 373 126 2 26 398 9 373 2.18e-168 476
MS.gene01364.t1 AT5G63980 44.507 355 169 6 23 377 3 329 1.27e-93 285
MS.gene01364.t1 AT5G09290 43.243 370 169 8 23 392 3 331 4.41e-88 270
MS.gene01364.t1 AT5G63990 39.437 355 184 9 23 377 3 326 7.22e-76 239
MS.gene01364.t1 AT5G64000 41.570 344 170 7 23 366 3 315 3.65e-74 235
MS.gene01364.t1 AT5G63990 37.072 321 172 8 23 343 3 293 9.79e-62 201
MS.gene01364.t1 AT5G64000 40.816 294 146 6 73 366 7 272 2.71e-60 197
MS.gene01364.t1 AT4G05090 30.730 397 201 14 22 392 45 393 4.44e-38 142
MS.gene01364.t1 AT4G05090 29.793 386 187 12 22 392 45 361 1.13e-34 132

Find 93 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCCGCATAGGGTAATT+TGG 0.135632 2.2:+69901060 None:intergenic
ACCATGCTGTATACTCTTAA+TGG 0.160691 2.2:+69899894 None:intergenic
GAATTTCCAAGGTCTGATTT+TGG 0.209004 2.2:+69901252 None:intergenic
TTGTGTGCTATATGCTAAAA+AGG 0.214002 2.2:-69900959 MS.gene01364:CDS
GAAGTAGTGCTTGGTGTTCT+TGG 0.249259 2.2:-69901087 MS.gene01364:CDS
ATCGTCTTCTTCTTCTGATT+TGG 0.252236 2.2:+69902597 None:intergenic
GTGGTTCCTCTGGAAGATTT+TGG 0.271726 2.2:-69901191 MS.gene01364:CDS
CGCGGTATAATTGCTTGTTC+TGG 0.292721 2.2:-69899684 MS.gene01364:CDS
TCAAAAGGATTATATTTAGA+AGG 0.294817 2.2:-69899714 MS.gene01364:CDS
CTGAACCTGATGATTGGTTT+TGG 0.301141 2.2:+69902486 None:intergenic
CTTGAATTGGCCTTCTCAAC+TGG 0.304582 2.2:+69900814 None:intergenic
AATTCTCCTGTTACTGTTGC+TGG 0.304788 2.2:-69902451 MS.gene01364:intron
GACTAACCAGCAACAGTAAC+AGG 0.314879 2.2:+69902445 None:intergenic
CAATCATCAGGTTCAGTCTA+AGG 0.320159 2.2:-69902479 MS.gene01364:CDS
TTGATGCTGTTGATGCTAGC+TGG 0.336054 2.2:-69899638 MS.gene01364:CDS
AAAATTTGTATTGCATGGTT+TGG 0.340323 2.2:+69900880 None:intergenic
TAGTTCAGATGCATTAGATT+TGG 0.341068 2.2:+69901341 None:intergenic
TGAAGAATATTCCAAGGAAT+TGG 0.352038 2.2:-69902575 MS.gene01364:CDS
GTTATCAACACCGGTATCAT+AGG 0.364679 2.2:-69901029 MS.gene01364:CDS
CAGGTTACAAGGAGAAAATA+TGG 0.379484 2.2:-69899815 MS.gene01364:CDS
TGGGGCTGTTCTAGCCTTTG+AGG 0.398273 2.2:-69899618 None:intergenic
AACTCAACCGGTGGTTCCTC+TGG 0.410607 2.2:-69901201 MS.gene01364:CDS
CTAGCTGAAGCGCCTCGATT+TGG 0.410609 2.2:-69901285 MS.gene01364:CDS
CTTAGACTGAACCTGATGAT+TGG 0.412831 2.2:+69902480 None:intergenic
GATTTCCAAAACCAATCATC+AGG 0.419970 2.2:-69902491 MS.gene01364:CDS
CTCCTCCAACTTCTGAAAGT+TGG 0.420854 2.2:-69900990 MS.gene01364:CDS
TGTTCTGGAGCCTCATTACA+TGG 0.432060 2.2:-69899669 MS.gene01364:CDS
AGTAAAAGAATGGCTTGAAT+TGG 0.437244 2.2:+69900801 None:intergenic
TGATGCTGTTGATGCTAGCT+GGG 0.442043 2.2:-69899637 MS.gene01364:CDS
TAACATTGACAATCCAGCTT+TGG 0.442559 2.2:-69900851 MS.gene01364:CDS
ATGGTAAAATATGCAGCAAT+AGG 0.443434 2.2:-69899876 MS.gene01364:CDS
TTGTTCCAACTTTCAGAAGT+TGG 0.452682 2.2:+69900985 None:intergenic
GACAGCAACATCCAATTCCT+TGG 0.453748 2.2:+69902564 None:intergenic
TTGCCCACAGTGTTGGTCTC+AGG 0.460819 2.2:-69900771 MS.gene01364:intron
TTAACCATTCCTTCCGCATA+GGG 0.463845 2.2:+69901052 None:intergenic
ATTTGCAAGGGCAGGTTACA+AGG 0.471911 2.2:-69899826 MS.gene01364:CDS
GTGTCCAAGCAGTTGTCAGC+TGG 0.480697 2.2:-69901431 MS.gene01364:CDS
ATAAGTCACTGCAACTCAAC+CGG 0.485262 2.2:-69901213 MS.gene01364:CDS
TTCATGAAATTTGCAAGGGC+AGG 0.487309 2.2:-69899834 MS.gene01364:CDS
GATCCTGTCGATGGTACGCT+CGG 0.488975 2.2:-69901162 MS.gene01364:CDS
GGTACGCTCGGGTTTGTTCG+TGG 0.514845 2.2:-69901150 MS.gene01364:CDS
ATCTTATTCATATGTAGCAA+TGG 0.523838 2.2:+69900913 None:intergenic
ATATCCAGCTGACAACTGCT+TGG 0.526105 2.2:+69901427 None:intergenic
AACCCGAGCGTACCATCGAC+AGG 0.527566 2.2:+69901159 None:intergenic
CTTAACCATTCCTTCCGCAT+AGG 0.528534 2.2:+69901051 None:intergenic
CAAGTCCTGCAGTAAAAGAA+TGG 0.531366 2.2:+69900791 None:intergenic
TTCACTTTGTCAGAGAGTGC+AGG 0.535716 2.2:-69902521 MS.gene01364:CDS
GCAGGACTTGCCCACAGTGT+TGG 0.536450 2.2:-69900778 MS.gene01364:CDS
GGTGGAGTGGTAACAGATGC+TGG 0.542538 2.2:-69899756 MS.gene01364:CDS
TTCACCTATCATGGAGGATG+AGG 0.542664 2.2:-69902644 None:intergenic
GTTATCATCATACAAGAAGC+TGG 0.542954 2.2:-69899777 MS.gene01364:CDS
TGCTGTCAGAGCAGTGCAAA+TGG 0.543521 2.2:-69902548 MS.gene01364:CDS
TGTCCCCTAGACTTCTCAAA+AGG 0.543780 2.2:-69899729 MS.gene01364:CDS
TCGATACAATCCTATGATAC+CGG 0.549696 2.2:+69901019 None:intergenic
CGAGAACTTCTGAATTTCCA+AGG 0.556247 2.2:+69901241 None:intergenic
AAAATATGGGATCATGCTGC+AGG 0.556967 2.2:-69899801 MS.gene01364:CDS
AGGTTACAAGGAGAAAATAT+GGG 0.562448 2.2:-69899814 MS.gene01364:CDS
CAAATCGAGGCGCTTCAGCT+AGG 0.564002 2.2:+69901286 None:intergenic
ATTACCCTATGCGGAAGGAA+TGG 0.571169 2.2:-69901056 MS.gene01364:CDS
AAAAGGGTACTGGAAAGGCT+TGG 0.572766 2.2:-69900942 MS.gene01364:CDS
AATGATCTTCCCATGTAATG+AGG 0.573603 2.2:+69899659 None:intergenic
TGGATATTATCTGAGTGTCT+AGG 0.576919 2.2:-69901411 MS.gene01364:CDS
AGACGATGAAGAATATTCCA+AGG 0.578169 2.2:-69902581 MS.gene01364:CDS
TGGGTACTGGATCCTGTCGA+TGG 0.582268 2.2:-69901171 MS.gene01364:CDS
GAGGATGGAGAAGTAGTGCT+TGG 0.585164 2.2:-69901096 MS.gene01364:CDS
AGGTATTCATGAAATTTGCA+AGG 0.586091 2.2:-69899839 MS.gene01364:CDS
TTCCAACTTTCAGAAGTTGG+AGG 0.587710 2.2:+69900988 None:intergenic
GCTCTAGCGCTCATAGAGGA+TGG 0.588697 2.2:-69901111 MS.gene01364:CDS
ACTTCTGAAAGTTGGAACAA+AGG 0.591206 2.2:-69900982 MS.gene01364:CDS
AAATATGCAGCAATAGGTCG+TGG 0.591339 2.2:-69899870 MS.gene01364:CDS
GGTATTCATGAAATTTGCAA+GGG 0.607340 2.2:-69899838 MS.gene01364:CDS
CAGAAGCCAAAATCAGACCT+TGG 0.608552 2.2:-69901258 MS.gene01364:CDS
GGCTTCTGAACACCAAATCG+AGG 0.616592 2.2:+69901273 None:intergenic
TCCAAATTACCCTATGCGGA+AGG 0.618727 2.2:-69901061 MS.gene01364:CDS
ATCCTGTCGATGGTACGCTC+GGG 0.623620 2.2:-69901161 MS.gene01364:CDS
GTTCTGGAGCCTCATTACAT+GGG 0.625091 2.2:-69899668 MS.gene01364:CDS
AATAGGTCGTGGTGATGCAG+AGG 0.627852 2.2:-69899859 MS.gene01364:CDS
AAATCTTCCAGAGGAACCAC+CGG 0.631669 2.2:+69901194 None:intergenic
CATACAAGAAGCTGGTGGAG+TGG 0.635866 2.2:-69899769 MS.gene01364:CDS
GATGCTGTTGATGCTAGCTG+GGG 0.654425 2.2:-69899636 MS.gene01364:CDS
AATGGTTAAGTTATCAACAC+CGG 0.658343 2.2:-69901038 MS.gene01364:CDS
GTACCTGAGACCAACACTGT+GGG 0.661258 2.2:+69900768 None:intergenic
ATCTGAACTATTAGACTCCG+TGG 0.662295 2.2:-69901328 MS.gene01364:CDS
TATTTAGAAGGCTTAGATCG+CGG 0.665083 2.2:-69899702 MS.gene01364:CDS
TGTAGCTCTAGCGCTCATAG+AGG 0.669638 2.2:-69901115 MS.gene01364:CDS
GCTGTCCAAATTACCCTATG+CGG 0.675859 2.2:-69901065 MS.gene01364:CDS
ATCATCATACAAGAAGCTGG+TGG 0.677049 2.2:-69899774 MS.gene01364:CDS
ACCATTAAGAGTATACAGCA+TGG 0.679685 2.2:-69899895 MS.gene01364:CDS
AGTCACTGCAACTCAACCGG+TGG 0.693702 2.2:-69901210 MS.gene01364:CDS
CAGTACCCAAAATCTTCCAG+AGG 0.703921 2.2:+69901185 None:intergenic
ATATGAATAAGATGTTTGTG+TGG 0.712325 2.2:-69900903 MS.gene01364:CDS
TGTACCTGAGACCAACACTG+TGG 0.715837 2.2:+69900767 None:intergenic
GGAGTGGTAACAGATGCTGG+AGG 0.815190 2.2:-69899753 MS.gene01364:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAATTCATGAATA+TGG - chr2.2:69901851-69901870 MS.gene01364:intron 10.0%
!! AAATATTAATGAAAAAGTTG+AGG - chr2.2:69900478-69900497 MS.gene01364:intron 15.0%
!!! CAATTTTTCAATCTTTTTTA+CGG + chr2.2:69902011-69902030 None:intergenic 15.0%
!! AGTTTACTGAAAATAATTAC+AGG - chr2.2:69901691-69901710 MS.gene01364:intron 20.0%
!! CAAAAGAATGATTATAAATG+AGG - chr2.2:69900287-69900306 MS.gene01364:intron 20.0%
!! GTTTACTGAAAATAATTACA+GGG - chr2.2:69901692-69901711 MS.gene01364:intron 20.0%
!! TATAAACCTATAATGTACTT+AGG + chr2.2:69902307-69902326 None:intergenic 20.0%
!! TCAAAAGGATTATATTTAGA+AGG - chr2.2:69902540-69902559 MS.gene01364:CDS 20.0%
!! TGTTAGTATATTAGTTGTTA+CGG - chr2.2:69899969-69899988 MS.gene01364:intron 20.0%
!!! TATGTTTCCATTTTTATATG+CGG - chr2.2:69900185-69900204 MS.gene01364:intron 20.0%
!!! TCTTTTTTACGGATATTAAT+AGG + chr2.2:69902000-69902019 None:intergenic 20.0%
! AAAATTGAACTCATTGGAAA+AGG - chr2.2:69902024-69902043 MS.gene01364:intron 25.0%
! AAAATTTGTATTGCATGGTT+TGG + chr2.2:69901377-69901396 None:intergenic 25.0%
! AAGAATGATTATAAATGAGG+AGG - chr2.2:69900290-69900309 MS.gene01364:intron 25.0%
! AAGTTAAAGTAACTTATACG+TGG + chr2.2:69900676-69900695 None:intergenic 25.0%
! AATTTGACCGCATATAAAAA+TGG + chr2.2:69900195-69900214 None:intergenic 25.0%
! AGACAAAAATTTGTATTGCA+TGG + chr2.2:69901382-69901401 None:intergenic 25.0%
! ATAGCGTAGAAATATAACAT+AGG + chr2.2:69900732-69900751 None:intergenic 25.0%
! ATATGAATAAGATGTTTGTG+TGG - chr2.2:69901351-69901370 MS.gene01364:CDS 25.0%
! ATCTTATTCATATGTAGCAA+TGG + chr2.2:69901344-69901363 None:intergenic 25.0%
! CTAGTGTAAACTAAAATCAT+TGG - chr2.2:69900219-69900238 MS.gene01364:intron 25.0%
! GAATAAGATGCAATCATAAT+TGG - chr2.2:69900579-69900598 MS.gene01364:intron 25.0%
! GATTGAAAAATTGAACTCAT+TGG - chr2.2:69902018-69902037 MS.gene01364:intron 25.0%
! TATCTTGTTGCAATTTATCT+GGG + chr2.2:69901824-69901843 None:intergenic 25.0%
! TGAAAATGTAAAATGAACAC+TGG + chr2.2:69900530-69900549 None:intergenic 25.0%
! TTTAGGCTATTATGTCTAAT+AGG + chr2.2:69900324-69900343 None:intergenic 25.0%
!! GCATGATATTCTTATTTACA+CGG - chr2.2:69901728-69901747 MS.gene01364:intron 25.0%
!! TATGTTATATTTCTACGCTA+TGG - chr2.2:69900731-69900750 MS.gene01364:intron 25.0%
!!! TCCACTAAACGATTTTTTAA+AGG + chr2.2:69900132-69900151 None:intergenic 25.0%
!!! TTCTTATTTTCACAGCAAAA+AGG + chr2.2:69900255-69900274 None:intergenic 25.0%
AACAGTTGACATATTAGCAT+TGG - chr2.2:69901766-69901785 MS.gene01364:intron 30.0%
AATGGTTAAGTTATCAACAC+CGG - chr2.2:69901216-69901235 MS.gene01364:CDS 30.0%
AGGTATTCATGAAATTTGCA+AGG - chr2.2:69902415-69902434 MS.gene01364:intron 30.0%
AGGTTACAAGGAGAAAATAT+GGG - chr2.2:69902440-69902459 MS.gene01364:intron 30.0%
AGTAAAAGAATGGCTTGAAT+TGG + chr2.2:69901456-69901475 None:intergenic 30.0%
ATGGTAAAATATGCAGCAAT+AGG - chr2.2:69902378-69902397 MS.gene01364:intron 30.0%
ATGTAAAATGAACACTGGAA+AGG + chr2.2:69900525-69900544 None:intergenic 30.0%
CTAAAAATTTACCTTGCAGT+AGG - chr2.2:69900339-69900358 MS.gene01364:intron 30.0%
CTATCTTGTTGCAATTTATC+TGG + chr2.2:69901825-69901844 None:intergenic 30.0%
GAAGAGGTTGTTGAATAAAA+TGG - chr2.2:69901908-69901927 MS.gene01364:intron 30.0%
GGTATTCATGAAATTTGCAA+GGG - chr2.2:69902416-69902435 MS.gene01364:intron 30.0%
TACAATGAGAAAGGAGATTA+TGG + chr2.2:69900637-69900656 None:intergenic 30.0%
TAGTTCAGATGCATTAGATT+TGG + chr2.2:69900916-69900935 None:intergenic 30.0%
TATAGCTGTTCTTAGGATTT+CGG - chr2.2:69900408-69900427 MS.gene01364:intron 30.0%
TCGTTTACGAGTTTATGTAT+AGG + chr2.2:69902250-69902269 None:intergenic 30.0%
TGAAGAATATTCCAAGGAAT+TGG - chr2.2:69899679-69899698 MS.gene01364:CDS 30.0%
TGATATGTCCATGATAGTTT+AGG - chr2.2:69902161-69902180 MS.gene01364:intron 30.0%
TGTGTGCTATATGCTAAAAA+GGG - chr2.2:69901296-69901315 MS.gene01364:CDS 30.0%
TTATTCATACAAATCCTCAG+TGG + chr2.2:69902072-69902091 None:intergenic 30.0%
TTGGAACTTTACAATGAGAA+AGG + chr2.2:69900646-69900665 None:intergenic 30.0%
TTGTGTGCTATATGCTAAAA+AGG - chr2.2:69901295-69901314 MS.gene01364:CDS 30.0%
! ATTCCAACGGATAATTTTTC+CGG - chr2.2:69900003-69900022 MS.gene01364:intron 30.0%
! CTACTGCAAGGTAAATTTTT+AGG + chr2.2:69900341-69900360 None:intergenic 30.0%
! GCCTTTAAAAAATCGTTTAG+TGG - chr2.2:69900128-69900147 MS.gene01364:intron 30.0%
!! AAGCATTTATGAGAGTGTTT+GGG - chr2.2:69902117-69902136 MS.gene01364:intron 30.0%
!! ATAATCCTTTTGAGAAGTCT+AGG + chr2.2:69902533-69902552 None:intergenic 30.0%
!! ATCATTGACAGTTTTAACCA+CGG + chr2.2:69900946-69900965 None:intergenic 30.0%
!! ATTGTGCTGTTTGAATAGAT+TGG - chr2.2:69900799-69900818 MS.gene01364:CDS 30.0%
!! GTTGTTTTCCTAAACTATCA+TGG + chr2.2:69902172-69902191 None:intergenic 30.0%
!! TAAGCATTTATGAGAGTGTT+TGG - chr2.2:69902116-69902135 MS.gene01364:intron 30.0%
!! TAATCCTTTTGAGAAGTCTA+GGG + chr2.2:69902532-69902551 None:intergenic 30.0%
!!! AGTTTTTTTATGGTTTGTGC+AGG - chr2.2:69902331-69902350 MS.gene01364:intron 30.0%
!!! TTTTTAAAGGCATCTTTGAC+TGG + chr2.2:69900119-69900138 None:intergenic 30.0%
AATCGAGCTGCTTATCTAAA+TGG - chr2.2:69902279-69902298 MS.gene01364:intron 35.0%
AATGATCTTCCCATGTAATG+AGG + chr2.2:69902598-69902617 None:intergenic 35.0%
ACAAAGATCAAGAGGTGAAA+AGG + chr2.2:69901562-69901581 None:intergenic 35.0%
ACCATGCTGTATACTCTTAA+TGG + chr2.2:69902363-69902382 None:intergenic 35.0%
ACTTATACGTGGTCATACAT+TGG + chr2.2:69900665-69900684 None:intergenic 35.0%
AGACGATGAAGAATATTCCA+AGG - chr2.2:69899673-69899692 MS.gene01364:CDS 35.0%
ATATTCCACATCTGAATGTC+TGG - chr2.2:69901958-69901977 MS.gene01364:intron 35.0%
ATCACACAACAAAGATCAAG+AGG + chr2.2:69901570-69901589 None:intergenic 35.0%
ATCGAGCTGCTTATCTAAAT+GGG - chr2.2:69902280-69902299 MS.gene01364:intron 35.0%
ATCGTCTTCTTCTTCTGATT+TGG + chr2.2:69899660-69899679 None:intergenic 35.0%
CAAAGATCAAGAGGTGAAAA+GGG + chr2.2:69901561-69901580 None:intergenic 35.0%
CAGGTTACAAGGAGAAAATA+TGG - chr2.2:69902439-69902458 MS.gene01364:intron 35.0%
CTCATGAGTGCTAATTTAAC+AGG + chr2.2:69901886-69901905 None:intergenic 35.0%
GAGAGAAAACAAGAGTCATA+TGG - chr2.2:69901604-69901623 MS.gene01364:intron 35.0%
GATTTCCAAAACCAATCATC+AGG - chr2.2:69899763-69899782 MS.gene01364:CDS 35.0%
GTTATCATCATACAAGAAGC+TGG - chr2.2:69902477-69902496 MS.gene01364:CDS 35.0%
GTTTATTTGGATGTGTGCAT+TGG - chr2.2:69899907-69899926 MS.gene01364:intron 35.0%
TAAAACCAGACATTCAGATG+TGG + chr2.2:69901966-69901985 None:intergenic 35.0%
TAACATTGACAATCCAGCTT+TGG - chr2.2:69901403-69901422 MS.gene01364:CDS 35.0%
TATTTAGAAGGCTTAGATCG+CGG - chr2.2:69902552-69902571 MS.gene01364:CDS 35.0%
TCGATACAATCCTATGATAC+CGG + chr2.2:69901238-69901257 None:intergenic 35.0%
TCTCATGTATAGCTGTTCTT+AGG - chr2.2:69900401-69900420 MS.gene01364:intron 35.0%
TGATTTCATTGTTGCCACAT+GGG - chr2.2:69899885-69899904 MS.gene01364:CDS 35.0%
TGGATATTATCTGAGTGTCT+AGG - chr2.2:69900843-69900862 MS.gene01364:CDS 35.0%
TTGTTCCAACTTTCAGAAGT+TGG + chr2.2:69901272-69901291 None:intergenic 35.0%
! AATCAAGCAAGGTGTTTACT+AGG + chr2.2:69900378-69900397 None:intergenic 35.0%
! ACCATTAAGAGTATACAGCA+TGG - chr2.2:69902359-69902378 MS.gene01364:intron 35.0%
! ACTTCTGAAAGTTGGAACAA+AGG - chr2.2:69901272-69901291 MS.gene01364:CDS 35.0%
! CTATATGCTAAAAAGGGTAC+TGG - chr2.2:69901302-69901321 MS.gene01364:CDS 35.0%
! GAATTTCCAAGGTCTGATTT+TGG + chr2.2:69901005-69901024 None:intergenic 35.0%
! TTAGTTGTTACGGCAGTTTT+CGG - chr2.2:69899979-69899998 MS.gene01364:intron 35.0%
! TTTTTGTGAACCAGTTGAGA+AGG - chr2.2:69901430-69901449 MS.gene01364:CDS 35.0%
!! AATCCTTTTGAGAAGTCTAG+GGG + chr2.2:69902531-69902550 None:intergenic 35.0%
!!! GTTAGTCTTTTTCCTGAACT+GGG - chr2.2:69899825-69899844 MS.gene01364:CDS 35.0%
AAAATATGGGATCATGCTGC+AGG - chr2.2:69902453-69902472 MS.gene01364:CDS 40.0%
AAATATGCAGCAATAGGTCG+TGG - chr2.2:69902384-69902403 MS.gene01364:intron 40.0%
AATTCTCCTGTTACTGTTGC+TGG - chr2.2:69899803-69899822 MS.gene01364:CDS 40.0%
ACACCGGAAAAATTATCCGT+TGG + chr2.2:69900009-69900028 None:intergenic 40.0%
ACATGAGATGCAATCAAGCA+AGG + chr2.2:69900389-69900408 None:intergenic 40.0%
ATAAGTCACTGCAACTCAAC+CGG - chr2.2:69901041-69901060 MS.gene01364:CDS 40.0%
ATCATCATACAAGAAGCTGG+TGG - chr2.2:69902480-69902499 MS.gene01364:CDS 40.0%
ATCTGAACTATTAGACTCCG+TGG - chr2.2:69900926-69900945 MS.gene01364:CDS 40.0%
ATGGGGCCTAAGTACATTAT+AGG - chr2.2:69902298-69902317 MS.gene01364:intron 40.0%
CAAGTCCTGCAGTAAAAGAA+TGG + chr2.2:69901466-69901485 None:intergenic 40.0%
CAATCATCAGGTTCAGTCTA+AGG - chr2.2:69899775-69899794 MS.gene01364:CDS 40.0%
CACATCCAAATAAACCCATG+TGG + chr2.2:69899902-69899921 None:intergenic 40.0%
CGAGAACTTCTGAATTTCCA+AGG + chr2.2:69901016-69901035 None:intergenic 40.0%
CTGATTTCATTGTTGCCACA+TGG - chr2.2:69899884-69899903 MS.gene01364:CDS 40.0%
CTTAGACTGAACCTGATGAT+TGG + chr2.2:69899777-69899796 None:intergenic 40.0%
GAAATGAAATGACCCAGTTC+AGG + chr2.2:69899840-69899859 None:intergenic 40.0%
GTTATCAACACCGGTATCAT+AGG - chr2.2:69901225-69901244 MS.gene01364:CDS 40.0%
TCGAGCTGCTTATCTAAATG+GGG - chr2.2:69902281-69902300 MS.gene01364:intron 40.0%
TCTACGCTATGGTTGTATGA+TGG - chr2.2:69900742-69900761 MS.gene01364:intron 40.0%
TGTGGCAACAATGAAATCAG+AGG + chr2.2:69899884-69899903 None:intergenic 40.0%
TGTTGCACAGTTGAAACACA+TGG + chr2.2:69901636-69901655 None:intergenic 40.0%
TGTTGCCACATGGGTTTATT+TGG - chr2.2:69899894-69899913 MS.gene01364:CDS 40.0%
TTAACCATTCCTTCCGCATA+GGG + chr2.2:69901205-69901224 None:intergenic 40.0%
TTAGCACTCATGAGATGAAG+AGG - chr2.2:69901892-69901911 MS.gene01364:intron 40.0%
TTCATGAAATTTGCAAGGGC+AGG - chr2.2:69902420-69902439 MS.gene01364:intron 40.0%
TTCCAACTTTCAGAAGTTGG+AGG + chr2.2:69901269-69901288 None:intergenic 40.0%
TTGGATGTGTGCATTGGAAT+TGG - chr2.2:69899913-69899932 MS.gene01364:intron 40.0%
! AGAGTGTTTGGGATAGCTTA+TGG - chr2.2:69902128-69902147 MS.gene01364:intron 40.0%
! CTCAACTGGTTCACAAAAAG+TGG + chr2.2:69901429-69901448 None:intergenic 40.0%
! GAGGATATTTTCCTACTGCA+AGG + chr2.2:69900353-69900372 None:intergenic 40.0%
! TCAAGCCATTCTTTTACTGC+AGG - chr2.2:69901458-69901477 MS.gene01364:intron 40.0%
!! TGCTAAAAAGGGTACTGGAA+AGG - chr2.2:69901307-69901326 MS.gene01364:CDS 40.0%
!! TGGTTCCTCTGGAAGATTTT+GGG - chr2.2:69901064-69901083 MS.gene01364:CDS 40.0%
!!! CTGAACCTGATGATTGGTTT+TGG + chr2.2:69899771-69899790 None:intergenic 40.0%
!!! GGTTAGTCTTTTTCCTGAAC+TGG - chr2.2:69899824-69899843 MS.gene01364:CDS 40.0%
AAATCTTCCAGAGGAACCAC+CGG + chr2.2:69901063-69901082 None:intergenic 45.0%
ATATCCAGCTGACAACTGCT+TGG + chr2.2:69900830-69900849 None:intergenic 45.0%
ATCAAGAGGTGAAAAGGGAG+AGG + chr2.2:69901556-69901575 None:intergenic 45.0%
ATTACCCTATGCGGAAGGAA+TGG - chr2.2:69901198-69901217 MS.gene01364:CDS 45.0%
ATTTGCAAGGGCAGGTTACA+AGG - chr2.2:69902428-69902447 MS.gene01364:intron 45.0%
CAGAAGCCAAAATCAGACCT+TGG - chr2.2:69900996-69901015 MS.gene01364:CDS 45.0%
CAGTACCCAAAATCTTCCAG+AGG + chr2.2:69901072-69901091 None:intergenic 45.0%
CGCGGTATAATTGCTTGTTC+TGG - chr2.2:69902570-69902589 MS.gene01364:CDS 45.0%
CTTAACCATTCCTTCCGCAT+AGG + chr2.2:69901206-69901225 None:intergenic 45.0%
GACAGCAACATCCAATTCCT+TGG + chr2.2:69899693-69899712 None:intergenic 45.0%
GACTAACCAGCAACAGTAAC+AGG + chr2.2:69899812-69899831 None:intergenic 45.0%
GCTGTCCAAATTACCCTATG+CGG - chr2.2:69901189-69901208 MS.gene01364:CDS 45.0%
GTGGCACAGTTATGCTATCT+TGG + chr2.2:69902053-69902072 None:intergenic 45.0%
TCACAAAAAGTGGCCAAAGC+TGG + chr2.2:69901419-69901438 None:intergenic 45.0%
TCCAAATTACCCTATGCGGA+AGG - chr2.2:69901193-69901212 MS.gene01364:CDS 45.0%
TCCTTCCGCATAGGGTAATT+TGG + chr2.2:69901197-69901216 None:intergenic 45.0%
TGATGCTGTTGATGCTAGCT+GGG - chr2.2:69902617-69902636 MS.gene01364:CDS 45.0%
TGTCCCCTAGACTTCTCAAA+AGG - chr2.2:69902525-69902544 MS.gene01364:CDS 45.0%
TTGATGCTGTTGATGCTAGC+TGG - chr2.2:69902616-69902635 MS.gene01364:CDS 45.0%
! ATAGCATAACTGTGCCACTG+AGG - chr2.2:69902055-69902074 MS.gene01364:intron 45.0%
! CTCCTCCAACTTCTGAAAGT+TGG - chr2.2:69901264-69901283 MS.gene01364:CDS 45.0%
! GACGCAGCTTTTTGTAGTAG+AGG - chr2.2:69900061-69900080 MS.gene01364:intron 45.0%
! GAGTAGACTCCACTAGTTTG+TGG + chr2.2:69900159-69900178 None:intergenic 45.0%
! GCAAGGTGTTTACTAGGAAG+AGG + chr2.2:69900372-69900391 None:intergenic 45.0%
! GGCAGTTTTCGGTATTCCAA+CGG - chr2.2:69899990-69900009 MS.gene01364:intron 45.0%
! TTCACTTTGTCAGAGAGTGC+AGG - chr2.2:69899733-69899752 MS.gene01364:CDS 45.0%
!! AAAAGGGTACTGGAAAGGCT+TGG - chr2.2:69901312-69901331 MS.gene01364:CDS 45.0%
!! CTCTGGAAGATTTTGGGTAC+TGG - chr2.2:69901070-69901089 MS.gene01364:CDS 45.0%
!! CTTGAATTGGCCTTCTCAAC+TGG + chr2.2:69901443-69901462 None:intergenic 45.0%
!! GAAGTAGTGCTTGGTGTTCT+TGG - chr2.2:69901167-69901186 MS.gene01364:CDS 45.0%
!! GTGGTTCCTCTGGAAGATTT+TGG - chr2.2:69901063-69901082 MS.gene01364:CDS 45.0%
!! GTTCTGGAGCCTCATTACAT+GGG - chr2.2:69902586-69902605 MS.gene01364:CDS 45.0%
!! TGTTCTGGAGCCTCATTACA+TGG - chr2.2:69902585-69902604 MS.gene01364:CDS 45.0%
!!! TTTATAATATAGTTTTTTTA+TGG - chr2.2:69902321-69902340 MS.gene01364:intron 5.0%
ACCAACCGAGCTGCTTACTT+GGG - chr2.2:69900039-69900058 MS.gene01364:intron 50.0%
CATACAAGAAGCTGGTGGAG+TGG - chr2.2:69902485-69902504 MS.gene01364:CDS 50.0%
GAGGATGGAGAAGTAGTGCT+TGG - chr2.2:69901158-69901177 MS.gene01364:CDS 50.0%
GATGCTGTTGATGCTAGCTG+GGG - chr2.2:69902618-69902637 MS.gene01364:CDS 50.0%
GGCTTCTGAACACCAAATCG+AGG + chr2.2:69900984-69901003 None:intergenic 50.0%
GTACCTGAGACCAACACTGT+GGG + chr2.2:69901489-69901508 None:intergenic 50.0%
GTGGAGACACCACAAACTAG+TGG - chr2.2:69900147-69900166 MS.gene01364:intron 50.0%
TACCAACCGAGCTGCTTACT+TGG - chr2.2:69900038-69900057 MS.gene01364:intron 50.0%
TGCTGTCAGAGCAGTGCAAA+TGG - chr2.2:69899706-69899725 MS.gene01364:CDS 50.0%
TGTACCTGAGACCAACACTG+TGG + chr2.2:69901490-69901509 None:intergenic 50.0%
! AATAGGTCGTGGTGATGCAG+AGG - chr2.2:69902395-69902414 MS.gene01364:intron 50.0%
! GTTGGTAAGCTAAGGGACAC+CGG + chr2.2:69900025-69900044 None:intergenic 50.0%
!! CAGCTCGGTTGGTAAGCTAA+GGG + chr2.2:69900032-69900051 None:intergenic 50.0%
!! TGTAGCTCTAGCGCTCATAG+AGG - chr2.2:69901139-69901158 MS.gene01364:CDS 50.0%
AACTCAACCGGTGGTTCCTC+TGG - chr2.2:69901053-69901072 MS.gene01364:CDS 55.0%
AGTCACTGCAACTCAACCGG+TGG - chr2.2:69901044-69901063 MS.gene01364:CDS 55.0%
ATCCTGTCGATGGTACGCTC+GGG - chr2.2:69901093-69901112 MS.gene01364:CDS 55.0%
GATCCTGTCGATGGTACGCT+CGG - chr2.2:69901092-69901111 MS.gene01364:CDS 55.0%
GCTCTAGCGCTCATAGAGGA+TGG - chr2.2:69901143-69901162 MS.gene01364:CDS 55.0%
GGAGTGGTAACAGATGCTGG+AGG - chr2.2:69902501-69902520 MS.gene01364:CDS 55.0%
GGTGGAGTGGTAACAGATGC+TGG - chr2.2:69902498-69902517 MS.gene01364:CDS 55.0%
GTGTCCAAGCAGTTGTCAGC+TGG - chr2.2:69900823-69900842 MS.gene01364:CDS 55.0%
TCCCAAGTAAGCAGCTCGGT+TGG + chr2.2:69900043-69900062 None:intergenic 55.0%
TGCGTCCCAAGTAAGCAGCT+CGG + chr2.2:69900047-69900066 None:intergenic 55.0%
! CAAATCGAGGCGCTTCAGCT+AGG + chr2.2:69900971-69900990 None:intergenic 55.0%
! CTAGCTGAAGCGCCTCGATT+TGG - chr2.2:69900969-69900988 MS.gene01364:CDS 55.0%
!! GCAGCTCGGTTGGTAAGCTA+AGG + chr2.2:69900033-69900052 None:intergenic 55.0%
!! TGGGTACTGGATCCTGTCGA+TGG - chr2.2:69901083-69901102 MS.gene01364:CDS 55.0%
!! TTGCCCACAGTGTTGGTCTC+AGG - chr2.2:69901483-69901502 MS.gene01364:intron 55.0%
AACCCGAGCGTACCATCGAC+AGG + chr2.2:69901098-69901117 None:intergenic 60.0%
GCAGGACTTGCCCACAGTGT+TGG - chr2.2:69901476-69901495 MS.gene01364:intron 60.0%
! GGTACGCTCGGGTTTGTTCG+TGG - chr2.2:69901104-69901123 MS.gene01364:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 69899620 69902656 69899620 ID=MS.gene01364
chr2.2 mRNA 69899620 69902656 69899620 ID=MS.gene01364.t1;Parent=MS.gene01364
chr2.2 exon 69902452 69902656 69902452 ID=MS.gene01364.t1.exon1;Parent=MS.gene01364.t1
chr2.2 CDS 69902452 69902656 69902452 ID=cds.MS.gene01364.t1;Parent=MS.gene01364.t1
chr2.2 exon 69900772 69901459 69900772 ID=MS.gene01364.t1.exon2;Parent=MS.gene01364.t1
chr2.2 CDS 69900772 69901459 69900772 ID=cds.MS.gene01364.t1;Parent=MS.gene01364.t1
chr2.2 exon 69899620 69899923 69899620 ID=MS.gene01364.t1.exon3;Parent=MS.gene01364.t1
chr2.2 CDS 69899620 69899923 69899620 ID=cds.MS.gene01364.t1;Parent=MS.gene01364.t1
Gene Sequence

>MS.gene01364

ATGGAGGATGAGGATCAGAAAAAGTTGAGTTTTTTCTCCAAATCAGAAGAAGAAGACGATGAAGAATATTCCAAGGAATTGGATGTTGCTGTCAGAGCAGTGCAAATGGCATGTTCACTTTGTCAGAGAGTGCAGGAAAGTTTGATTTCCAAAACCAATCATCAGGTTCAGTCTAAGGATGATAATTCTCCTGTTACTGTTGCTGGTTAGTCTTTTTCCTGAACTGGGTCATTTCATTTCATATACAAGTTCTTTATTTTTCCTCTGATTTCATTGTTGCCACATGGGTTTATTTGGATGTGTGCATTGGAATTGGATATTATATTGTCTTGCATCAAATTTCTAGAAATGTTAGTATATTAGTTGTTACGGCAGTTTTCGGTATTCCAACGGATAATTTTTCCGGTGTCCCTTAGCTTACCAACCGAGCTGCTTACTTGGGACGCAGCTTTTTGTAGTAGAGGTTTATTTAGTGAACTTAGCAATTATACAAACTCCAGTCAAAGATGCCTTTAAAAAATCGTTTAGTGGAGACACCACAAACTAGTGGAGTCTACTCTTTGTCTATGTTTCCATTTTTATATGCGGTCAAATTATGACTAGTGTAAACTAAAATCATTGGTGTGAAATATCCTTTTTGCTGTGAAAATAAGAAATTCTATTGAAACAAAAGAATGATTATAAATGAGGAGGATAAGTATCCTATTAGACATAATAGCCTAAAAATTTACCTTGCAGTAGGAAAATATCCTCTTCCTAGTAAACACCTTGCTTGATTGCATCTCATGTATAGCTGTTCTTAGGATTTCGGAAGCACATTTATGCTTTTGAAAGAATACAAGTTTTCAAAAGATTGAAAAATATTAATGAAAAAGTTGAGGTGATTTTTCAATTTATTTAGACCTTTCCAGTGTTCATTTTACATTTTCAACTTTACATGTTGCTATTAGCATTGAATTGAATAAGATGCAATCATAATTGGAATCATGATCTTCATAATTAATTTATTACATACCATAATCTCCTTTCTCATTGTAAAGTTCCAATGTATGACCACGTATAAGTTACTTTAACTTGAGCAAAGATCTATGATTAAATTTTTGCTGTGTCCTATGTTATATTTCTACGCTATGGTTGTATGATGGTTGAAGCTTTTCTGAGTTTTGTACATAAAATTTCATTGTGCTGTTTGAATAGATTGGAGTGTCCAAGCAGTTGTCAGCTGGATATTATCTGAGTGTCTAGGAAGCGAAAATATCTCAATTGTAGCTGAAGAAGATGTTCAAACTCTCTCCAAATCTAATGCATCTGAACTATTAGACTCCGTGGTTAAAACTGTCAATGATTGCCTAGCTGAAGCGCCTCGATTTGGTGTTCAGAAGCCAAAATCAGACCTTGGAAATTCAGAAGTTCTCGATATTATAAGTCACTGCAACTCAACCGGTGGTTCCTCTGGAAGATTTTGGGTACTGGATCCTGTCGATGGTACGCTCGGGTTTGTTCGTGGCGATCAATATGCTGTAGCTCTAGCGCTCATAGAGGATGGAGAAGTAGTGCTTGGTGTTCTTGGCTGTCCAAATTACCCTATGCGGAAGGAATGGTTAAGTTATCAACACCGGTATCATAGGATTGTATCGAAGTTGACTCCTCCAACTTCTGAAAGTTGGAACAAAGGTTGTGTGCTATATGCTAAAAAGGGTACTGGAAAGGCTTGGATGCAACCATTGCTACATATGAATAAGATGTTTGTGTGGCCAAACCATGCAATACAAATTTTTGTCTCTAACATTGACAATCCAGCTTTGGCCACTTTTTGTGAACCAGTTGAGAAGGCCAATTCAAGCCATTCTTTTACTGCAGGACTTGCCCACAGTGTTGGTCTCAGGTACATTTTTCTTTTTTTCAATTTCATTTTGATATCAATTACTCTCTGCCTCTCCCTTTTCACCTCTTGATCTTTGTTGTGTGATCATCGATGTTGAAAGAGAGAAAACAAGAGTCATATGGTGAATACCATGTGTTTCAACTGTGCAACAGAGATTCTTCATATAGATTTAAATTTGTTTGTGAAAGTTTACTGAAAATAATTACAGGGTATTCTACTCGTTGCATGATATTCTTATTTACACGGTCATATTTTATTTTCAACAGTTGACATATTAGCATTGGATTATTATGATGTTCTTGTTCGTTTTAAATATCCCAGATAAATTGCAACAAGATAGATTCTAAAATTTAAAATTCATGAATATGGTCGAATAATCCTGTTAAATTAGCACTCATGAGATGAAGAGGTTGTTGAATAAAATGGAAAGCAGAAACTGTGATGTGTAGTTTAATATTCCACATCTGAATGTCTGGTTTTACTAGTTAGATTCCTATTAATATCCGTAAAAAAGATTGAAAAATTGAACTCATTGGAAAAGGTCACCAAGATAGCATAACTGTGCCACTGAGGATTTGTATGAATAACTTATTTGTGTTTATTTACTAGAATAAGCATTTATGAGAGTGTTTGGGATAGCTTATGGAAATAACTTATGATATGTCCATGATAGTTTAGGAAAACAACTTATAGCTTATGTGAAAACAGATTGAATTTATTTTATAATTTGTTATAGAAATAGCCTATACATAAACTCGTAAACGATAAGCGCTTAATCGAGCTGCTTATCTAAATGGGGCCTAAGTACATTATAGGTTTATAATATAGTTTTTTTATGGTTTGTGCAGGAAACAACCATTAAGAGTATACAGCATGGTAAAATATGCAGCAATAGGTCGTGGTGATGCAGAGGTATTCATGAAATTTGCAAGGGCAGGTTACAAGGAGAAAATATGGGATCATGCTGCAGGTGTTATCATCATACAAGAAGCTGGTGGAGTGGTAACAGATGCTGGAGGATGTCCCCTAGACTTCTCAAAAGGATTATATTTAGAAGGCTTAGATCGCGGTATAATTGCTTGTTCTGGAGCCTCATTACATGGGAAGATCATTGATGCTGTTGATGCTAGCTGGGGCTGTTCTAGCCTTTGA
Protein sequence

>MS.gene01364.t1

MEDEDQKKLSFFSKSEEEDDEEYSKELDVAVRAVQMACSLCQRVQESLISKTNHQVQSKDDNSPVTVADWSVQAVVSWILSECLGSENISIVAEEDVQTLSKSNASELLDSVVKTVNDCLAEAPRFGVQKPKSDLGNSEVLDIISHCNSTGGSSGRFWVLDPVDGTLGFVRGDQYAVALALIEDGEVVLGVLGCPNYPMRKEWLSYQHRYHRIVSKLTPPTSESWNKGCVLYAKKGTGKAWMQPLLHMNKMFVWPNHAIQIFVSNIDNPALATFCEPVEKANSSHSFTAGLAHSVGLRKQPLRVYSMVKYAAIGRGDAEVFMKFARAGYKEKIWDHAAGVIIIQEAGGVVTDAGGCPLDFSKGLYLEGLDRGIIACSGASLHGKIIDAVDASWGCSSL