Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014619.t1 | XP_003603638.1 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 2.80E-95 | 357.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014619.t1 | P59082 | 35.8 | 162 | 90 | 5 | 8 | 168 | 20 | 168 | 2.8e-19 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014619.t1 | G7J3I2 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 2.0e-95 | 357.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64289 | MS.gene014619 | PPI |
| MS.gene014619 | MS.gene94983 | PPI |
| MS.gene014619 | MS.gene53620 | PPI |
| MS.gene014619 | MS.gene064976 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014619.t1 | MTR_3g109880 | 96.532 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 8.25e-123 | 343 |
| MS.gene014619.t1 | MTR_6g088445 | 41.718 | 163 | 84 | 3 | 3 | 164 | 5 | 157 | 1.03e-39 | 132 |
| MS.gene014619.t1 | MTR_6g088475 | 38.650 | 163 | 89 | 3 | 3 | 164 | 5 | 157 | 7.09e-36 | 123 |
| MS.gene014619.t1 | MTR_1g046660 | 36.000 | 175 | 103 | 3 | 1 | 173 | 1 | 168 | 4.51e-34 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014619.t1 | AT4G32870 | 49.701 | 167 | 70 | 4 | 1 | 165 | 1 | 155 | 2.93e-55 | 172 |
| MS.gene014619.t1 | AT2G25770 | 39.157 | 166 | 89 | 4 | 1 | 164 | 1 | 156 | 1.69e-36 | 124 |
| MS.gene014619.t1 | AT2G25770 | 39.157 | 166 | 89 | 4 | 1 | 164 | 1 | 156 | 1.69e-36 | 124 |
Find 39 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGACTTTACCTTCCCATTT+TGG | 0.196562 | 3.1:+88250487 | None:intergenic |
| TATTGTGCTTCTAACATAAA+AGG | 0.298692 | 3.1:-88250331 | MS.gene014619:CDS |
| GAGAGGAATCTATACCAAAA+TGG | 0.300079 | 3.1:-88250501 | MS.gene014619:CDS |
| TGTAATGTCCACCCTTCTAT+TGG | 0.303893 | 3.1:+88250091 | None:intergenic |
| TGAGTACTGTCTTCAGTTCA+TGG | 0.311338 | 3.1:-88250050 | MS.gene014619:CDS |
| ACACCGTTGAAGAACAAGTA+TGG | 0.334958 | 3.1:-88250450 | MS.gene014619:CDS |
| TAGGACAAACAACGTTTGAT+TGG | 0.364301 | 3.1:+88250232 | None:intergenic |
| ACATGGTAACATGTGTCTAT+TGG | 0.380795 | 3.1:+88250388 | None:intergenic |
| GTTGTTCTCAACAATTTCAT+AGG | 0.401740 | 3.1:+88250213 | None:intergenic |
| TTTGTTTGTGATCCAATAGA+AGG | 0.405699 | 3.1:-88250103 | MS.gene014619:CDS |
| GCCCACTTGATCGTTGTCTC+AGG | 0.413163 | 3.1:+88250280 | None:intergenic |
| ATTTCTGCAACCTACACAAA+TGG | 0.433647 | 3.1:-88250414 | MS.gene014619:CDS |
| AGAGGAATCTATACCAAAAT+GGG | 0.442772 | 3.1:-88250500 | MS.gene014619:CDS |
| TACCATGTTGAAGGTGTTCA+AGG | 0.460746 | 3.1:-88250373 | MS.gene014619:CDS |
| GAAGGTGATGCTAAAAGTGC+AGG | 0.494052 | 3.1:-88250145 | MS.gene014619:CDS |
| GTTCATGGCTAAGAAGATTG+AGG | 0.515780 | 3.1:-88250035 | MS.gene014619:CDS |
| GACACATGTTACCATGTTGA+AGG | 0.516605 | 3.1:-88250382 | MS.gene014619:CDS |
| GTGCAGGGTGTGGGATTGAG+TGG | 0.523829 | 3.1:-88250129 | MS.gene014619:CDS |
| CTATTGGAATCCATTTGTGT+AGG | 0.531264 | 3.1:+88250404 | None:intergenic |
| TGCCCATACTTGTTCTTCAA+CGG | 0.556176 | 3.1:+88250447 | None:intergenic |
| GAAGGTGTTCAAGGCCAACC+TGG | 0.564455 | 3.1:-88250364 | MS.gene014619:CDS |
| CACCGTTGAAGAACAAGTAT+GGG | 0.572196 | 3.1:-88250449 | MS.gene014619:CDS |
| GCACAATAGCGTATGAGACC+AGG | 0.576223 | 3.1:+88250346 | None:intergenic |
| AGCCTGAGACAACGATCAAG+TGG | 0.582251 | 3.1:-88250282 | MS.gene014619:CDS |
| ATGCTAAAAGTGCAGGGTGT+GGG | 0.582963 | 3.1:-88250138 | MS.gene014619:CDS |
| TTGTTTGTGATCCAATAGAA+GGG | 0.583616 | 3.1:-88250102 | MS.gene014619:CDS |
| GGCCTTGAACACCTTCAACA+TGG | 0.588245 | 3.1:+88250371 | None:intergenic |
| TGCAGGGTGTGGGATTGAGT+GGG | 0.598045 | 3.1:-88250128 | MS.gene014619:CDS |
| GAAATTGTTGAGAACAACAT+GGG | 0.605096 | 3.1:-88250208 | MS.gene014619:CDS |
| TTTGTGATCCAATAGAAGGG+TGG | 0.611273 | 3.1:-88250099 | MS.gene014619:CDS |
| GATGCTAAAAGTGCAGGGTG+TGG | 0.614021 | 3.1:-88250139 | MS.gene014619:CDS |
| GCCTGAGACAACGATCAAGT+GGG | 0.623769 | 3.1:-88250281 | MS.gene014619:CDS |
| ATCGGCGAGAGAGATGGGAG+AGG | 0.639114 | 3.1:-88250518 | None:intergenic |
| GAATCTATACCAAAATGGGA+AGG | 0.656789 | 3.1:-88250496 | MS.gene014619:CDS |
| CTGAAAGTACTTTCAAACGA+AGG | 0.658207 | 3.1:-88250163 | MS.gene014619:CDS |
| AAGGTGATGCTAAAAGTGCA+GGG | 0.666240 | 3.1:-88250144 | MS.gene014619:CDS |
| GGAAGGTAAAGTCACTGTTG+AGG | 0.667190 | 3.1:-88250479 | MS.gene014619:CDS |
| TGAAATTGTTGAGAACAACA+TGG | 0.671799 | 3.1:-88250209 | MS.gene014619:CDS |
| AATAGCGTATGAGACCAGGT+TGG | 0.712406 | 3.1:+88250350 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTGTGCTTCTAACATAAA+AGG | - | chr3.1:88250180-88250199 | MS.gene014619:CDS | 25.0% |
| AGAGGAATCTATACCAAAAT+GGG | - | chr3.1:88250011-88250030 | MS.gene014619:CDS | 30.0% | |
| GAAATTGTTGAGAACAACAT+GGG | - | chr3.1:88250303-88250322 | MS.gene014619:CDS | 30.0% | |
| GTTGTTCTCAACAATTTCAT+AGG | + | chr3.1:88250301-88250320 | None:intergenic | 30.0% | |
| TGAAATTGTTGAGAACAACA+TGG | - | chr3.1:88250302-88250321 | MS.gene014619:CDS | 30.0% | |
| TTGTTTGTGATCCAATAGAA+GGG | - | chr3.1:88250409-88250428 | MS.gene014619:CDS | 30.0% | |
| TTTGTTTGTGATCCAATAGA+AGG | - | chr3.1:88250408-88250427 | MS.gene014619:CDS | 30.0% | |
| ACATGGTAACATGTGTCTAT+TGG | + | chr3.1:88250126-88250145 | None:intergenic | 35.0% | |
| ATTTCTGCAACCTACACAAA+TGG | - | chr3.1:88250097-88250116 | MS.gene014619:CDS | 35.0% | |
| CTATTGGAATCCATTTGTGT+AGG | + | chr3.1:88250110-88250129 | None:intergenic | 35.0% | |
| GAATCTATACCAAAATGGGA+AGG | - | chr3.1:88250015-88250034 | MS.gene014619:CDS | 35.0% | |
| GAGAGGAATCTATACCAAAA+TGG | - | chr3.1:88250010-88250029 | MS.gene014619:CDS | 35.0% | |
| TAGGACAAACAACGTTTGAT+TGG | + | chr3.1:88250282-88250301 | None:intergenic | 35.0% | |
| !! | CTGAAAGTACTTTCAAACGA+AGG | - | chr3.1:88250348-88250367 | MS.gene014619:CDS | 35.0% |
| ACACCGTTGAAGAACAAGTA+TGG | - | chr3.1:88250061-88250080 | MS.gene014619:CDS | 40.0% | |
| CACCGTTGAAGAACAAGTAT+GGG | - | chr3.1:88250062-88250081 | MS.gene014619:CDS | 40.0% | |
| GACACATGTTACCATGTTGA+AGG | - | chr3.1:88250129-88250148 | MS.gene014619:CDS | 40.0% | |
| GTTCATGGCTAAGAAGATTG+AGG | - | chr3.1:88250476-88250495 | MS.gene014619:CDS | 40.0% | |
| TGCCCATACTTGTTCTTCAA+CGG | + | chr3.1:88250067-88250086 | None:intergenic | 40.0% | |
| TGTAATGTCCACCCTTCTAT+TGG | + | chr3.1:88250423-88250442 | None:intergenic | 40.0% | |
| TTTGTGATCCAATAGAAGGG+TGG | - | chr3.1:88250412-88250431 | MS.gene014619:CDS | 40.0% | |
| ! | AAGGTGATGCTAAAAGTGCA+GGG | - | chr3.1:88250367-88250386 | MS.gene014619:CDS | 40.0% |
| ! | GTGACTTTACCTTCCCATTT+TGG | + | chr3.1:88250027-88250046 | None:intergenic | 40.0% |
| ! | TGAGTACTGTCTTCAGTTCA+TGG | - | chr3.1:88250461-88250480 | MS.gene014619:CDS | 40.0% |
| !! | TACCATGTTGAAGGTGTTCA+AGG | - | chr3.1:88250138-88250157 | MS.gene014619:CDS | 40.0% |
| AATAGCGTATGAGACCAGGT+TGG | + | chr3.1:88250164-88250183 | None:intergenic | 45.0% | |
| GGAAGGTAAAGTCACTGTTG+AGG | - | chr3.1:88250032-88250051 | MS.gene014619:CDS | 45.0% | |
| ! | ATGCTAAAAGTGCAGGGTGT+GGG | - | chr3.1:88250373-88250392 | MS.gene014619:CDS | 45.0% |
| ! | GAAGGTGATGCTAAAAGTGC+AGG | - | chr3.1:88250366-88250385 | MS.gene014619:CDS | 45.0% |
| AGCCTGAGACAACGATCAAG+TGG | - | chr3.1:88250229-88250248 | MS.gene014619:CDS | 50.0% | |
| GCACAATAGCGTATGAGACC+AGG | + | chr3.1:88250168-88250187 | None:intergenic | 50.0% | |
| GCCTGAGACAACGATCAAGT+GGG | - | chr3.1:88250230-88250249 | MS.gene014619:CDS | 50.0% | |
| GGCCTTGAACACCTTCAACA+TGG | + | chr3.1:88250143-88250162 | None:intergenic | 50.0% | |
| ! | GATGCTAAAAGTGCAGGGTG+TGG | - | chr3.1:88250372-88250391 | MS.gene014619:CDS | 50.0% |
| GCCCACTTGATCGTTGTCTC+AGG | + | chr3.1:88250234-88250253 | None:intergenic | 55.0% | |
| TGCAGGGTGTGGGATTGAGT+GGG | - | chr3.1:88250383-88250402 | MS.gene014619:CDS | 55.0% | |
| !! | GAAGGTGTTCAAGGCCAACC+TGG | - | chr3.1:88250147-88250166 | MS.gene014619:CDS | 55.0% |
| GTGCAGGGTGTGGGATTGAG+TGG | - | chr3.1:88250382-88250401 | MS.gene014619:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 88250006 | 88250527 | 88250006 | ID=MS.gene014619 |
| chr3.1 | mRNA | 88250006 | 88250527 | 88250006 | ID=MS.gene014619.t1;Parent=MS.gene014619 |
| chr3.1 | exon | 88250006 | 88250527 | 88250006 | ID=MS.gene014619.t1.exon1;Parent=MS.gene014619.t1 |
| chr3.1 | CDS | 88250006 | 88250527 | 88250006 | ID=cds.MS.gene014619.t1;Parent=MS.gene014619.t1 |
| Gene Sequence |
| Protein sequence |
