AlfalfaGEDB | MS.gene64289

M. sativa cultivar XinJiangDaYe / MS.gene64289

KEGG: -
GO: -
PFAM: PF10604.12

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64289.t1	XP_013467174.1	93	171	8	2	1	167	1	171	2.10E-76	295

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64289.t1	P59082	36.6	153	94	2	4	156	16	165	3.4e-22	106.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64289.t1	I3SDX8	93.0	171	8	2	1	167	1	171	1.5e-76	295.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene64289	MS.gene014619	PPI
MS.gene64289	MS.gene40272	PPI
MS.gene64289	MS.gene014621	PPI
MS.gene64289	MS.gene40715	PPI
MS.gene64289	MS.gene40266	PPI
MS.gene64289	MS.gene72031	PPI
MS.gene64289	MS.gene72034	PPI
MS.gene64289	MS.gene40267	PPI
MS.gene64289	MS.gene40716	PPI
MS.gene64289	MS.gene014620	PPI
MS.gene64289	MS.gene72033	PPI
MS.gene64289	MS.gene40717	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64289.t1	MTR_1g046660	93.567	171	7	2	1	167	1	171	1.91e-104	296
MS.gene64289.t1	MTR_3g109880	37.791	172	94	4	1	161	1	170	3.77e-38	129
MS.gene64289.t1	MTR_6g088445	35.802	162	96	2	3	160	5	162	5.13e-36	123
MS.gene64289.t1	MTR_6g088475	36.420	162	95	2	3	160	5	162	5.64e-35	120
MS.gene64289.t1	MTR_5g081780	26.619	139	98	2	24	161	24	159	3.07e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64289.t1	AT2G25770	50.000	154	71	2	7	157	8	158	1.11e-51	163
MS.gene64289.t1	AT2G25770	50.000	154	71	2	7	157	8	158	1.11e-51	163
MS.gene64289.t1	AT4G32870	39.597	149	86	3	8	155	9	154	5.09e-31	110

Find 56 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGATGGGGATGGTGGGTG+TGG	0.223284	1.3:-28323733	MS.gene64289:CDS
TGTGGGGTTGAGTGGTTCTT+TGG	0.230418	1.3:-28323715	MS.gene64289:CDS
TCAAGTTATGGCTCAGAAAA+TGG	0.286347	1.3:-28323632	MS.gene64289:CDS
GAGATTGTTGAGTGTAATAT+TGG	0.313988	1.3:-28323799	MS.gene64289:CDS
CGTAACACGTGGCGAGGTTT+GGG	0.334834	1.3:+28323956	None:intergenic
GTGATGGGGATGGTGGGTGT+GGG	0.338627	1.3:-28323732	MS.gene64289:CDS
CCGTAACACGTGGCGAGGTT+TGG	0.344859	1.3:+28323955	None:intergenic
ATGGTGGGTGTGGGGTTGAG+TGG	0.347376	1.3:-28323723	MS.gene64289:CDS
GGTGATGGTGATGGGGATGG+TGG	0.372095	1.3:-28323739	MS.gene64289:CDS
TGATGGGGATGGTGGGTGTG+GGG	0.372487	1.3:-28323731	MS.gene64289:CDS
TTGTTGATGGTGATGGTGAT+GGG	0.375171	1.3:-28323747	MS.gene64289:CDS
GTTGTTGATGGTGATGGTGA+TGG	0.385145	1.3:-28323748	MS.gene64289:CDS
CCAAACCTCGCCACGTGTTA+CGG	0.392203	1.3:-28323955	MS.gene64289:CDS
TCGTGTTGGGCTTCAAGTTA+TGG	0.394949	1.3:-28323644	MS.gene64289:CDS
TTTGGTGTTGATCCTGTTGA+AGG	0.395954	1.3:-28323697	MS.gene64289:CDS
TTTCAGTTTGGTTGAAACCT+TGG	0.405600	1.3:+28324041	None:intergenic
TGTTGAAGGGTTGGAGTTTG+AGG	0.413260	1.3:-28323683	MS.gene64289:CDS
CAGCTCACTTCTTGTGATGA+TGG	0.430118	1.3:+28323874	None:intergenic
GTGATGGTGATGGGGATGGT+GGG	0.432935	1.3:-28323738	MS.gene64289:CDS
GATGGTGATGGTGATGGGGA+TGG	0.434342	1.3:-28323742	MS.gene64289:CDS
TCAAACTCCAACCCTTCAAC+AGG	0.436235	1.3:+28323685	None:intergenic
AACAAAACATTATTCAAAGA+TGG	0.437627	1.3:-28324068	MS.gene64289:CDS
AGGGTTGGAGTTTGAGGAGT+TGG	0.460105	1.3:-28323677	MS.gene64289:CDS
TCTAGCTACATTTCATTGCT+AGG	0.467241	1.3:+28323586	None:intergenic
GAGCTGGTCAAAGGAGAGAT+TGG	0.472491	1.3:-28323857	MS.gene64289:CDS
TGTTGATGGTGATGGTGATG+GGG	0.477314	1.3:-28323746	MS.gene64289:CDS
AGCTCACTTCTTGTGATGAT+GGG	0.493172	1.3:+28323875	None:intergenic
TGTTGATCCTGTTGAAGGGT+TGG	0.507759	1.3:-28323692	MS.gene64289:CDS
TGGTGAGTAAGTATCGTGTT+GGG	0.510095	1.3:-28323657	MS.gene64289:CDS
CACTTCTTGTGATGATGGGA+GGG	0.524394	1.3:+28323879	None:intergenic
AGATTGTTGAGTGTAATATT+GGG	0.525385	1.3:-28323798	MS.gene64289:CDS
TTGGTGTTGATCCTGTTGAA+GGG	0.529067	1.3:-28323696	MS.gene64289:CDS
ACAAAACATTATTCAAAGAT+GGG	0.531963	1.3:-28324067	MS.gene64289:CDS
TTGGTGAGTAAGTATCGTGT+TGG	0.536250	1.3:-28323658	MS.gene64289:CDS
ACACAACAAAACAACAAGCA+TGG	0.539590	1.3:-28324017	MS.gene64289:CDS
CCACCTCACCGTTGGATCCG+TGG	0.545923	1.3:+28323929	None:intergenic
TACGGTATCCACGGATCCAA+CGG	0.553320	1.3:-28323937	MS.gene64289:CDS
CCACGGATCCAACGGTGAGG+TGG	0.559455	1.3:-28323929	MS.gene64289:CDS
TATTCAAAGATGGGAAGCCA+AGG	0.563664	1.3:-28324058	MS.gene64289:CDS
CATCATCACAAGAAGTGAGC+TGG	0.573089	1.3:-28323873	MS.gene64289:CDS
TCAACTATGAGGGTTGTTGA+TGG	0.573221	1.3:-28323760	MS.gene64289:CDS
CACGGATCCAACGGTGAGGT+GGG	0.577602	1.3:-28323928	MS.gene64289:CDS
ATGAGGGTTGTTGATGGTGA+TGG	0.579949	1.3:-28323754	MS.gene64289:CDS
TCACTTCTTGTGATGATGGG+AGG	0.583329	1.3:+28323878	None:intergenic
TATGCATCCCACCTCACCGT+TGG	0.583601	1.3:+28323921	None:intergenic
GGATGCATACGTTACTGTGC+TGG	0.591609	1.3:-28323907	MS.gene64289:CDS
ACAAGAAGTGAGCTGGTCAA+AGG	0.594280	1.3:-28323866	MS.gene64289:CDS
TTCTTGTGATGATGGGAGGG+AGG	0.627581	1.3:+28323882	None:intergenic
ACTCAAGTCGACTTCATCAA+CGG	0.634209	1.3:+28323831	None:intergenic
GCCACGTGTTACGGTATCCA+CGG	0.641652	1.3:-28323946	MS.gene64289:CDS
AGTGTTATGAGTCAACTATG+AGG	0.643248	1.3:-28323771	MS.gene64289:CDS
GTGTTATGAGTCAACTATGA+GGG	0.655476	1.3:-28323770	MS.gene64289:CDS
GGATACCGTAACACGTGGCG+AGG	0.661937	1.3:+28323950	None:intergenic
AGTTATGGCTCAGAAAATGG+AGG	0.665948	1.3:-28323629	MS.gene64289:CDS
TCCGTGGATACCGTAACACG+TGG	0.704216	1.3:+28323945	None:intergenic
TATCCACGGATCCAACGGTG+AGG	0.759312	1.3:-28323932	MS.gene64289:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACAAAACATTATTCAAAGA+TGG	-	chr1.3:28323595-28323614	MS.gene64289:CDS	20.0%
!!	ACAAAACATTATTCAAAGAT+GGG	-	chr1.3:28323596-28323615	MS.gene64289:CDS	20.0%
!	AGATTGTTGAGTGTAATATT+GGG	-	chr1.3:28323865-28323884	MS.gene64289:CDS	25.0%
!!!	TTTTGTTGTGTTTTTCAGTT+TGG	+	chr1.3:28323637-28323656	None:intergenic	25.0%
	GAGATTGTTGAGTGTAATAT+TGG	-	chr1.3:28323864-28323883	MS.gene64289:CDS	30.0%
	TTGAAAAAGTCTTTGATGAG+TGG	+	chr1.3:28323672-28323691	None:intergenic	30.0%
	ACACAACAAAACAACAAGCA+TGG	-	chr1.3:28323646-28323665	MS.gene64289:CDS	35.0%
	AGTGTTATGAGTCAACTATG+AGG	-	chr1.3:28323892-28323911	MS.gene64289:CDS	35.0%
	GTGTTATGAGTCAACTATGA+GGG	-	chr1.3:28323893-28323912	MS.gene64289:CDS	35.0%
	TCAAGTTATGGCTCAGAAAA+TGG	-	chr1.3:28324031-28324050	MS.gene64289:CDS	35.0%
	TTTCAGTTTGGTTGAAACCT+TGG	+	chr1.3:28323625-28323644	None:intergenic	35.0%
	AGCTCACTTCTTGTGATGAT+GGG	+	chr1.3:28323791-28323810	None:intergenic	40.0%
	AGTTATGGCTCAGAAAATGG+AGG	-	chr1.3:28324034-28324053	MS.gene64289:CDS	40.0%
	TATTCAAAGATGGGAAGCCA+AGG	-	chr1.3:28323605-28323624	MS.gene64289:CDS	40.0%
	TCAACTATGAGGGTTGTTGA+TGG	-	chr1.3:28323903-28323922	MS.gene64289:CDS	40.0%
!	ACTCAAGTCGACTTCATCAA+CGG	+	chr1.3:28323835-28323854	None:intergenic	40.0%
!	TGGTGAGTAAGTATCGTGTT+GGG	-	chr1.3:28324006-28324025	MS.gene64289:CDS	40.0%
!	TTGGTGAGTAAGTATCGTGT+TGG	-	chr1.3:28324005-28324024	MS.gene64289:CDS	40.0%
!!	TTGGTGTTGATCCTGTTGAA+GGG	-	chr1.3:28323967-28323986	MS.gene64289:CDS	40.0%
!!	TTGTTGATGGTGATGGTGAT+GGG	-	chr1.3:28323916-28323935	MS.gene64289:CDS	40.0%
!!	TTTGGTGTTGATCCTGTTGA+AGG	-	chr1.3:28323966-28323985	MS.gene64289:CDS	40.0%
	ACAAGAAGTGAGCTGGTCAA+AGG	-	chr1.3:28323797-28323816	MS.gene64289:CDS	45.0%
	CAGCTCACTTCTTGTGATGA+TGG	+	chr1.3:28323792-28323811	None:intergenic	45.0%
	CATCATCACAAGAAGTGAGC+TGG	-	chr1.3:28323790-28323809	MS.gene64289:CDS	45.0%
	TCAAACTCCAACCCTTCAAC+AGG	+	chr1.3:28323981-28324000	None:intergenic	45.0%
	TGTTGAAGGGTTGGAGTTTG+AGG	-	chr1.3:28323980-28323999	MS.gene64289:CDS	45.0%
	TGTTGATCCTGTTGAAGGGT+TGG	-	chr1.3:28323971-28323990	MS.gene64289:CDS	45.0%
!	TCGTGTTGGGCTTCAAGTTA+TGG	-	chr1.3:28324019-28324038	MS.gene64289:CDS	45.0%
!!	ATGAGGGTTGTTGATGGTGA+TGG	-	chr1.3:28323909-28323928	MS.gene64289:CDS	45.0%
!!	CACTTCTTGTGATGATGGGA+GGG	+	chr1.3:28323787-28323806	None:intergenic	45.0%
!!	GTTGTTGATGGTGATGGTGA+TGG	-	chr1.3:28323915-28323934	MS.gene64289:CDS	45.0%
!!	TCACTTCTTGTGATGATGGG+AGG	+	chr1.3:28323788-28323807	None:intergenic	45.0%
!!	TGTTGATGGTGATGGTGATG+GGG	-	chr1.3:28323917-28323936	MS.gene64289:CDS	45.0%
	AGGGTTGGAGTTTGAGGAGT+TGG	-	chr1.3:28323986-28324005	MS.gene64289:CDS	50.0%
	GAGCTGGTCAAAGGAGAGAT+TGG	-	chr1.3:28323806-28323825	MS.gene64289:CDS	50.0%
	GGATGCATACGTTACTGTGC+TGG	-	chr1.3:28323756-28323775	MS.gene64289:CDS	50.0%
	TACGGTATCCACGGATCCAA+CGG	-	chr1.3:28323726-28323745	MS.gene64289:CDS	50.0%
	TGTGGGGTTGAGTGGTTCTT+TGG	-	chr1.3:28323948-28323967	MS.gene64289:CDS	50.0%
!	CGAGGTTTGGGAAGCTTTTG+TGG	+	chr1.3:28323698-28323717	None:intergenic	50.0%
!!	TTCTTGTGATGATGGGAGGG+AGG	+	chr1.3:28323784-28323803	None:intergenic	50.0%
	CCAAACCTCGCCACGTGTTA+CGG	-	chr1.3:28323708-28323727	MS.gene64289:CDS	55.0%
	CGTAACACGTGGCGAGGTTT+GGG	+	chr1.3:28323710-28323729	None:intergenic	55.0%
	GCCACGTGTTACGGTATCCA+CGG	-	chr1.3:28323717-28323736	MS.gene64289:CDS	55.0%
	TATCCACGGATCCAACGGTG+AGG	-	chr1.3:28323731-28323750	MS.gene64289:CDS	55.0%
	TATGCATCCCACCTCACCGT+TGG	+	chr1.3:28323745-28323764	None:intergenic	55.0%
	TCCGTGGATACCGTAACACG+TGG	+	chr1.3:28323721-28323740	None:intergenic	55.0%
!!	GATGGTGATGGTGATGGGGA+TGG	-	chr1.3:28323921-28323940	MS.gene64289:CDS	55.0%
!!	GTGATGGTGATGGGGATGGT+GGG	-	chr1.3:28323925-28323944	MS.gene64289:CDS	55.0%
	CACGGATCCAACGGTGAGGT+GGG	-	chr1.3:28323735-28323754	MS.gene64289:CDS	60.0%
	CCGTAACACGTGGCGAGGTT+TGG	+	chr1.3:28323711-28323730	None:intergenic	60.0%
	GGATACCGTAACACGTGGCG+AGG	+	chr1.3:28323716-28323735	None:intergenic	60.0%
!!	ATGGTGGGTGTGGGGTTGAG+TGG	-	chr1.3:28323940-28323959	MS.gene64289:CDS	60.0%
!!	GGTGATGGTGATGGGGATGG+TGG	-	chr1.3:28323924-28323943	MS.gene64289:CDS	60.0%
!!	GTGATGGGGATGGTGGGTGT+GGG	-	chr1.3:28323931-28323950	MS.gene64289:CDS	60.0%
!!	TGATGGGGATGGTGGGTGTG+GGG	-	chr1.3:28323932-28323951	MS.gene64289:CDS	60.0%
	CCACCTCACCGTTGGATCCG+TGG	+	chr1.3:28323737-28323756	None:intergenic	65.0%
	CCACGGATCCAACGGTGAGG+TGG	-	chr1.3:28323734-28323753	MS.gene64289:CDS	65.0%
!!	GGTGATGGGGATGGTGGGTG+TGG	-	chr1.3:28323930-28323949	MS.gene64289:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	28323591	28324094	28323591	ID=MS.gene64289
chr1.3	mRNA	28323591	28324094	28323591	ID=MS.gene64289.t1;Parent=MS.gene64289
chr1.3	exon	28323591	28324094	28323591	ID=MS.gene64289.t1.exon1;Parent=MS.gene64289.t1
chr1.3	CDS	28323591	28324094	28323591	ID=cds.MS.gene64289.t1;Parent=MS.gene64289.t1

Gene Sequence

>MS.gene64289

ATGGAACAAAACATTATTCAAAGATGGGAAGCCAAGGTTTCAACCAAACTGAAAAACACAACAAAACAACAAGCATGGCCACTCATCAAAGACTTTTTCAATCTCCACAAAAGCTTCCCAAACCTCGCCACGTGTTACGGTATCCACGGATCCAACGGTGAGGTGGGATGCATACGTTACTGTGCTGGATCCTCCCTCCCATCATCACAAGAAGTGAGCTGGTCAAAGGAGAGATTGGTAGCCGTTGATGAAGTCGACTTGAGTATTAAGTATGAGATTGTTGAGTGTAATATTGGGTTTGAGTGTTATGAGTCAACTATGAGGGTTGTTGATGGTGATGGTGATGGGGATGGTGGGTGTGGGGTTGAGTGGTTCTTTGGTGTTGATCCTGTTGAAGGGTTGGAGTTTGAGGAGTTGGTGAGTAAGTATCGTGTTGGGCTTCAAGTTATGGCTCAGAAAATGGAGGAAGAGATTGCATCTTCACTTCCTAGCAATGAAATGTAG

Protein sequence

>MS.gene64289.t1

MEQNIIQRWEAKVSTKLKNTTKQQAWPLIKDFFNLHKSFPNLATCYGIHGSNGEVGCIRYCAGSSLPSSQEVSWSKERLVAVDEVDLSIKYEIVECNIGFECYESTMRVVDGDGDGDGGCGVEWFFGVDPVEGLEFEELVSKYRVGLQVMAQKMEEEIASSLPSNEM