AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94983


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94983.t1 MTR_1g046660 83.544 158 19 3 1 152 1 157 3.50e-83 243
MS.gene94983.t1 MTR_6g088475 40.141 142 76 3 4 138 2 141 7.05e-33 115
MS.gene94983.t1 MTR_6g088445 36.765 136 80 2 7 138 8 141 1.62e-32 114
MS.gene94983.t1 MTR_3g109880 37.662 154 75 5 9 149 8 153 2.38e-31 111
MS.gene94983.t1 MTR_5g081780 26.446 121 87 2 25 144 24 143 6.96e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94983.t1 AT2G25770 50.685 146 68 2 1 143 1 145 4.02e-48 154
MS.gene94983.t1 AT2G25770 50.685 146 68 2 1 143 1 145 4.02e-48 154
MS.gene94983.t1 AT4G32870 39.286 140 81 3 1 138 1 138 1.66e-28 103

Find 53 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATTCAGCAGCTTGATAA+AGG 0.284231 1.1:-29546367 MS.gene94983:CDS
TGTGTGGTTGAGTGGTTCTT+TGG 0.310083 1.1:-29547312 MS.gene94983:CDS
GAGATTGTTGAGTGTAATAT+TGG 0.313988 1.1:-29547390 MS.gene94983:CDS
TTTGGTGTTGATCCTGTTGA+TGG 0.333130 1.1:-29547294 MS.gene94983:CDS
TGGGTTGGAGTTTGAGGAAT+TGG 0.359808 1.1:-29547274 MS.gene94983:intron
CGTAACACGTGGCGAGGTTT+GGG 0.377149 1.1:+29547547 None:intergenic
CCAAACCTCGCCACGTGTTA+CGG 0.382744 1.1:-29547546 MS.gene94983:CDS
GTTGATGGTGATGGGGATGG+TGG 0.384072 1.1:-29547336 MS.gene94983:CDS
CCGTAACACGTGGCGAGGTT+TGG 0.387173 1.1:+29547546 None:intergenic
CGAGGTTTGGGAAACGTTTG+TGG 0.407906 1.1:+29547559 None:intergenic
TTGATGGTGATGGGGATGGT+GGG 0.417523 1.1:-29547335 MS.gene94983:CDS
GTTGTTGATGGTGATGGGGA+TGG 0.424168 1.1:-29547339 MS.gene94983:CDS
CAGCTCACTTCTTGTGATGA+TGG 0.430118 1.1:+29547465 None:intergenic
AAAACATTATTCAACAAAGA+TGG 0.432233 1.1:-29547659 MS.gene94983:CDS
TTGATAAAGGGTTTGAAGCC+TGG 0.440985 1.1:-29546354 MS.gene94983:CDS
TGAGGGTTGTTGATGGTGAT+GGG 0.445607 1.1:-29547344 MS.gene94983:CDS
TCAAACTCCAACCCATCAAC+AGG 0.450047 1.1:+29547282 None:intergenic
GAATTCAGCAGCTTGATAAA+GGG 0.451709 1.1:-29546366 MS.gene94983:CDS
TGTTGATGGGTTGGAGTTTG+AGG 0.456481 1.1:-29547280 MS.gene94983:CDS
ATGAGGGTTGTTGATGGTGA+TGG 0.472202 1.1:-29547345 MS.gene94983:CDS
GAGCTGGTCAAAGGAGAGAT+TGG 0.472491 1.1:-29547448 MS.gene94983:CDS
TGTTGATCCTGTTGATGGGT+TGG 0.478872 1.1:-29547289 MS.gene94983:CDS
ATGGTGGGTGTGTGGTTGAG+TGG 0.487454 1.1:-29547320 MS.gene94983:CDS
GCCAGTGATGTAGTGACTCC+AGG 0.491135 1.1:+29546336 None:intergenic
AGCTCACTTCTTGTGATGAT+GGG 0.493172 1.1:+29547466 None:intergenic
TGATGGGGATGGTGGGTGTG+TGG 0.509639 1.1:-29547328 MS.gene94983:CDS
TTGGTGTTGATCCTGTTGAT+GGG 0.518074 1.1:-29547293 MS.gene94983:CDS
CACTTCTTGTGATGATGGGA+GGG 0.524394 1.1:+29547470 None:intergenic
AGATTGTTGAGTGTAATATT+GGG 0.525385 1.1:-29547389 MS.gene94983:CDS
AAACATTATTCAACAAAGAT+GGG 0.536088 1.1:-29547658 MS.gene94983:CDS
ACACCACAAAACAACAAGCA+TGG 0.539590 1.1:-29547608 MS.gene94983:CDS
GCCTGGAGTCACTACATCAC+TGG 0.545431 1.1:-29546337 MS.gene94983:CDS
GAGGGTTGTTGATGGTGATG+GGG 0.548055 1.1:-29547343 MS.gene94983:CDS
GGATGCATACGCTACTGTGC+AGG 0.554173 1.1:-29547498 MS.gene94983:CDS
TCAACAAAGATGGGAAGCCA+AGG 0.555814 1.1:-29547649 MS.gene94983:CDS
CCACCTCACCGTTGGATCCG+TGG 0.560691 1.1:+29547520 None:intergenic
CCACGGATCCAACGGTGAGG+TGG 0.561622 1.1:-29547520 MS.gene94983:CDS
CATCATCACAAGAAGTGAGC+TGG 0.573089 1.1:-29547464 MS.gene94983:CDS
TCAACTATGAGGGTTGTTGA+TGG 0.573221 1.1:-29547351 MS.gene94983:CDS
CACGGATCCAACGGTGAGGT+GGG 0.580655 1.1:-29547519 MS.gene94983:CDS
TCACTTCTTGTGATGATGGG+AGG 0.583329 1.1:+29547469 None:intergenic
TATGCATCCCACCTCACCGT+TGG 0.586048 1.1:+29547512 None:intergenic
TACGGTGTCCACGGATCCAA+CGG 0.591176 1.1:-29547528 MS.gene94983:CDS
GCCACGTGTTACGGTGTCCA+CGG 0.592143 1.1:-29547537 MS.gene94983:CDS
ACAAGAAGTGAGCTGGTCAA+AGG 0.594280 1.1:-29547457 MS.gene94983:CDS
TTGAAGAAGTCTTTGATGAG+TGG 0.598134 1.1:+29547585 None:intergenic
ACTCAAGTCAATATCATCAA+CGG 0.624395 1.1:+29547422 None:intergenic
TTCTTGTGATGATGGGAGGG+AGG 0.627581 1.1:+29547473 None:intergenic
TCCGTGGACACCGTAACACG+TGG 0.643143 1.1:+29547536 None:intergenic
AGTGTTATGAGTCAACTATG+AGG 0.643248 1.1:-29547362 MS.gene94983:CDS
GGACACCGTAACACGTGGCG+AGG 0.651096 1.1:+29547541 None:intergenic
GTGTTATGAGTCAACTATGA+GGG 0.655476 1.1:-29547361 MS.gene94983:CDS
TGTCCACGGATCCAACGGTG+AGG 0.739687 1.1:-29547523 MS.gene94983:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATATAATTTATTCATT+TGG + chr1.1:29547098-29547117 None:intergenic 10.0%
!! ATTACAATATGTAAAAAGAT+CGG + chr1.1:29546874-29546893 None:intergenic 15.0%
!! TAAATTGCAACAAAAATTAA+GGG + chr1.1:29547045-29547064 None:intergenic 15.0%
!!! AAATTTCATATTGATTAACT+TGG + chr1.1:29547190-29547209 None:intergenic 15.0%
!! AAAACATTATTCAACAAAGA+TGG - chr1.1:29546330-29546349 MS.gene94983:CDS 20.0%
!! AAACATTATTCAACAAAGAT+GGG - chr1.1:29546331-29546350 MS.gene94983:CDS 20.0%
!! CAATAGTCAAATAAAACAAA+TGG - chr1.1:29546971-29546990 MS.gene94983:intron 20.0%
!! CATATAATTTATTCATTTGG+AGG + chr1.1:29547095-29547114 None:intergenic 20.0%
!! CTAAATTGCAACAAAAATTA+AGG + chr1.1:29547046-29547065 None:intergenic 20.0%
!! TTACAATATGTAAAAAGATC+GGG + chr1.1:29546873-29546892 None:intergenic 20.0%
!!! TCTGTTTTTAAGCTAATTTT+TGG - chr1.1:29547068-29547087 MS.gene94983:intron 20.0%
! AGATTGTTGAGTGTAATATT+GGG - chr1.1:29546600-29546619 MS.gene94983:intron 25.0%
! ATTAGAAGTTCATGAATAAG+TGG - chr1.1:29546830-29546849 MS.gene94983:intron 25.0%
! CATAGGGAAATAAATAAACT+AGG + chr1.1:29546937-29546956 None:intergenic 25.0%
! TTCATGAATAAGTGGAAATT+CGG - chr1.1:29546838-29546857 MS.gene94983:intron 25.0%
! TTTATTTATTTCCCTATGTG+TGG - chr1.1:29546939-29546958 MS.gene94983:intron 25.0%
!!! TTAGTCTTACATTTGCTTTT+AGG + chr1.1:29547241-29547260 None:intergenic 25.0%
AAAGCAAATGTAAGACTAAC+AGG - chr1.1:29547242-29547261 MS.gene94983:intron 30.0%
AACAATCAACAAAATGCTTC+AGG - chr1.1:29547503-29547522 MS.gene94983:CDS 30.0%
ACTCAAGTCAATATCATCAA+CGG + chr1.1:29546570-29546589 None:intergenic 30.0%
CACAAATCTAATTTACACGT+AGG - chr1.1:29547327-29547346 MS.gene94983:CDS 30.0%
GAGATTGTTGAGTGTAATAT+TGG - chr1.1:29546599-29546618 MS.gene94983:intron 30.0%
GTATCTATTTCAACTTGAGT+TGG + chr1.1:29547376-29547395 None:intergenic 30.0%
GTTAATTGAGTTATGTTCGT+GGG - chr1.1:29546899-29546918 MS.gene94983:intron 30.0%
TCTAGTTGCATTTCATTCTT+AGG + chr1.1:29546809-29546828 None:intergenic 30.0%
TGATCTATTAACCACACATA+GGG + chr1.1:29546953-29546972 None:intergenic 30.0%
TGTTAATTGAGTTATGTTCG+TGG - chr1.1:29546898-29546917 MS.gene94983:intron 30.0%
TTGATCTATTAACCACACAT+AGG + chr1.1:29546954-29546973 None:intergenic 30.0%
TTTGACAAAAACACAACACA+TGG + chr1.1:29547122-29547141 None:intergenic 30.0%
! ATGATCCTTAATTTTCCTGA+AGG + chr1.1:29547577-29547596 None:intergenic 30.0%
! TCATTTTACACAACTTTCTC+AGG - chr1.1:29547538-29547557 MS.gene94983:CDS 30.0%
!!! TTTGAGTTTTGTTGAAACCT+TGG + chr1.1:29546360-29546379 None:intergenic 30.0%
AGAATTCAGCAGCTTGATAA+AGG - chr1.1:29547622-29547641 MS.gene94983:CDS 35.0%
AGTGTTATGAGTCAACTATG+AGG - chr1.1:29546627-29546646 MS.gene94983:intron 35.0%
GAATTCAGCAGCTTGATAAA+GGG - chr1.1:29547623-29547642 MS.gene94983:CDS 35.0%
GAGATCCTTCAGGAAAATTA+AGG - chr1.1:29547569-29547588 MS.gene94983:CDS 35.0%
GTGTTATGAGTCAACTATGA+GGG - chr1.1:29546628-29546647 MS.gene94983:intron 35.0%
TCAAGTTATGGCTCAGAAAA+TGG - chr1.1:29546760-29546779 MS.gene94983:intron 35.0%
TTGAAGAAGTCTTTGATGAG+TGG + chr1.1:29546407-29546426 None:intergenic 35.0%
! TTCATAGATGTTACTTGTGC+AGG + chr1.1:29547449-29547468 None:intergenic 35.0%
ACACCACAAAACAACAAGCA+TGG - chr1.1:29546381-29546400 MS.gene94983:CDS 40.0%
AGCTCACTTCTTGTGATGAT+GGG + chr1.1:29546526-29546545 None:intergenic 40.0%
AGTTATGGCTCAGAAAATGG+AGG - chr1.1:29546763-29546782 MS.gene94983:intron 40.0%
TCAACTATGAGGGTTGTTGA+TGG - chr1.1:29546638-29546657 MS.gene94983:intron 40.0%
TGTTGTTAAGAGTCCCACAT+CGG + chr1.1:29547487-29547506 None:intergenic 40.0%
! ACAACTTTCTCAGGCTTTTG+GGG - chr1.1:29547547-29547566 MS.gene94983:CDS 40.0%
! ACACAACTTTCTCAGGCTTT+TGG - chr1.1:29547545-29547564 MS.gene94983:CDS 40.0%
! CACAACTTTCTCAGGCTTTT+GGG - chr1.1:29547546-29547565 MS.gene94983:CDS 40.0%
! TGGTGAGTAAGTATCGTGTT+GGG - chr1.1:29546735-29546754 MS.gene94983:intron 40.0%
! TTGATAAAGGGTTTGAAGCC+TGG - chr1.1:29547635-29547654 MS.gene94983:CDS 40.0%
! TTGGTGAGTAAGTATCGTGT+TGG - chr1.1:29546734-29546753 MS.gene94983:intron 40.0%
!! TTGGTGTTGATCCTGTTGAT+GGG - chr1.1:29546696-29546715 MS.gene94983:intron 40.0%
!! TTTGGTGTTGATCCTGTTGA+TGG - chr1.1:29546695-29546714 MS.gene94983:intron 40.0%
ACAAGAAGTGAGCTGGTCAA+AGG - chr1.1:29546532-29546551 MS.gene94983:intron 45.0%
CACTTACTCCTATCCGATGT+GGG - chr1.1:29547471-29547490 MS.gene94983:CDS 45.0%
CAGCTCACTTCTTGTGATGA+TGG + chr1.1:29546527-29546546 None:intergenic 45.0%
CATCATCACAAGAAGTGAGC+TGG - chr1.1:29546525-29546544 MS.gene94983:intron 45.0%
TCAAACTCCAACCCATCAAC+AGG + chr1.1:29546710-29546729 None:intergenic 45.0%
TCAACAAAGATGGGAAGCCA+AGG - chr1.1:29546340-29546359 MS.gene94983:CDS 45.0%
TCACTTACTCCTATCCGATG+TGG - chr1.1:29547470-29547489 MS.gene94983:CDS 45.0%
TGTGTGGTTGAGTGGTTCTT+TGG - chr1.1:29546677-29546696 MS.gene94983:intron 45.0%
TTAAGAGTCCCACATCGGAT+AGG + chr1.1:29547482-29547501 None:intergenic 45.0%
! TCGTGTTGGGCTTCAAGTTA+TGG - chr1.1:29546748-29546767 MS.gene94983:intron 45.0%
! TGGGTTGGAGTTTGAGGAAT+TGG - chr1.1:29546715-29546734 MS.gene94983:intron 45.0%
! TGTTGATCCTGTTGATGGGT+TGG - chr1.1:29546700-29546719 MS.gene94983:intron 45.0%
!! ATGAGGGTTGTTGATGGTGA+TGG - chr1.1:29546644-29546663 MS.gene94983:intron 45.0%
!! CACTTCTTGTGATGATGGGA+GGG + chr1.1:29546522-29546541 None:intergenic 45.0%
!! TCACTTCTTGTGATGATGGG+AGG + chr1.1:29546523-29546542 None:intergenic 45.0%
!! TGAGGGTTGTTGATGGTGAT+GGG - chr1.1:29546645-29546664 MS.gene94983:intron 45.0%
!! TGTTGATGGGTTGGAGTTTG+AGG - chr1.1:29546709-29546728 MS.gene94983:intron 45.0%
!!! TGGCCATGCTTGTTGTTTTG+TGG + chr1.1:29546387-29546406 None:intergenic 45.0%
CGAGGTTTGGGAAACGTTTG+TGG + chr1.1:29546433-29546452 None:intergenic 50.0%
GAGCTGGTCAAAGGAGAGAT+TGG - chr1.1:29546541-29546560 MS.gene94983:intron 50.0%
!! GAGGGTTGTTGATGGTGATG+GGG - chr1.1:29546646-29546665 MS.gene94983:intron 50.0%
!! GTTGTTGATGGTGATGGGGA+TGG - chr1.1:29546650-29546669 MS.gene94983:intron 50.0%
!! TTCTTGTGATGATGGGAGGG+AGG + chr1.1:29546519-29546538 None:intergenic 50.0%
!! TTGATGGTGATGGGGATGGT+GGG - chr1.1:29546654-29546673 MS.gene94983:intron 50.0%
CCAAACCTCGCCACGTGTTA+CGG - chr1.1:29546443-29546462 MS.gene94983:intron 55.0%
CGTAACACGTGGCGAGGTTT+GGG + chr1.1:29546445-29546464 None:intergenic 55.0%
GCCAGTGATGTAGTGACTCC+AGG + chr1.1:29547656-29547675 None:intergenic 55.0%
GCCTGGAGTCACTACATCAC+TGG - chr1.1:29547652-29547671 MS.gene94983:CDS 55.0%
GGATGCATACGCTACTGTGC+AGG - chr1.1:29546491-29546510 MS.gene94983:intron 55.0%
TACGGTGTCCACGGATCCAA+CGG - chr1.1:29546461-29546480 MS.gene94983:intron 55.0%
TATGCATCCCACCTCACCGT+TGG + chr1.1:29546480-29546499 None:intergenic 55.0%
!! ATGGTGGGTGTGTGGTTGAG+TGG - chr1.1:29546669-29546688 MS.gene94983:intron 55.0%
!! GGCTTTTGGGGAGATCCTTC+AGG - chr1.1:29547559-29547578 MS.gene94983:CDS 55.0%
!! GTTGATGGTGATGGGGATGG+TGG - chr1.1:29546653-29546672 MS.gene94983:intron 55.0%
CACGGATCCAACGGTGAGGT+GGG - chr1.1:29546470-29546489 MS.gene94983:intron 60.0%
CCGTAACACGTGGCGAGGTT+TGG + chr1.1:29546446-29546465 None:intergenic 60.0%
GCCACGTGTTACGGTGTCCA+CGG - chr1.1:29546452-29546471 MS.gene94983:intron 60.0%
TCCGTGGACACCGTAACACG+TGG + chr1.1:29546456-29546475 None:intergenic 60.0%
TGTCCACGGATCCAACGGTG+AGG - chr1.1:29546466-29546485 MS.gene94983:intron 60.0%
!! TGATGGGGATGGTGGGTGTG+TGG - chr1.1:29546661-29546680 MS.gene94983:intron 60.0%
CCACCTCACCGTTGGATCCG+TGG + chr1.1:29546472-29546491 None:intergenic 65.0%
CCACGGATCCAACGGTGAGG+TGG - chr1.1:29546469-29546488 MS.gene94983:intron 65.0%
GGACACCGTAACACGTGGCG+AGG + chr1.1:29546451-29546470 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 29546323 29547688 29546323 ID=MS.gene94983
chr1.1 mRNA 29546323 29547688 29546323 ID=MS.gene94983.t1;Parent=MS.gene94983
chr1.1 exon 29547275 29547688 29547275 ID=MS.gene94983.t1.exon1;Parent=MS.gene94983.t1
chr1.1 CDS 29547275 29547688 29547275 ID=cds.MS.gene94983.t1;Parent=MS.gene94983.t1
chr1.1 exon 29546323 29546400 29546323 ID=MS.gene94983.t1.exon2;Parent=MS.gene94983.t1
chr1.1 CDS 29546323 29546400 29546323 ID=cds.MS.gene94983.t1;Parent=MS.gene94983.t1
Gene Sequence

>MS.gene94983

ATGGAACAAAACATTATTCAACAAAGATGGGAAGCCAAGGTTTCAACAAAACTCAAAAACACCACAAAACAACAAGCATGGCCACTCATCAAAGACTTCTTCAATATCCACAAACGTTTCCCAAACCTCGCCACGTGTTACGGTGTCCACGGATCCAACGGTGAGGTGGGATGCATACGCTACTGTGCAGGATCCTCCCTCCCATCATCACAAGAAGTGAGCTGGTCAAAGGAGAGATTGGTAGCCGTTGATGATATTGACTTGAGTATTAAGTATGAGATTGTTGAGTGTAATATTGGGTTTGAGTGTTATGAGTCAACTATGAGGGTTGTTGATGGTGATGGGGATGGTGGGTGTGTGGTTGAGTGGTTCTTTGGTGTTGATCCTGTTGATGGGTTGGAGTTTGAGGAATTGGTGAGTAAGTATCGTGTTGGGCTTCAAGTTATGGCTCAGAAAATGGAGGAAGAGATTGCATCTTCACTTCCTAAGAATGAAATGCAACTAGAAATTAGAAGTTCATGAATAAGTGGAAATTCGGAGTTTCAATCCCGATCTTTTTACATATTGTAATTTAATGTTAATTGAGTTATGTTCGTGGGATTCATCGTAATCCTAGTTTATTTATTTCCCTATGTGTGGTTAATAGATCAATAGTCAAATAAAACAAATGGTTAAATAAGTTTTTTTGTCTTCGTAATGTCTCAAATTTTGTTTTTAGTCCCTTAATTTTTGTTGCAATTTAGTCTCTGTTTTTAAGCTAATTTTTGGTCCTCCAAATGAATAAATTATATGATTTCCATGTGTTGTGTTTTTGTCAAATACTAAAAACAATTTAACAGATTCGCATATCAACATTTAGACATGCCAAGTTAATCAATATGAAATTTATTTCGAATTGTGTTTTTATCATGTCAACCTAAAAGCAAATGTAAGACTAACAGGTTAAAAATGTGTAAAAATAGAATATGAAGTTCTATTGATGTAAGCAATAGTATTGATCAAATCACAAATCTAATTTACACGTAGGTTAAAAATTGTGTTTTACTCTAACCAACTCAAGTTGAAATAGATACTAACTGTAGCTAGAAAATTGTTAACAAATTGTTAGATAGTTGTCTAACTTCCTGCACAAGTAACATCTATGAACTCACTTACTCCTATCCGATGTGGGACTCTTAACAACAATCAACAAAATGCTTCAGGAAAATTAAGTCTTCATTTTACACAACTTTCTCAGGCTTTTGGGGAGATCCTTCAGGAAAATTAAGGATCATTAAAATATTTTAAGTGTGAGAAACAAGAATTCAGCAGCTTGATAAAGGGTTTGAAGCCTGGAGTCACTACATCACTGGCTCTTTTCAATTAA

Protein sequence

>MS.gene94983.t1

MEQNIIQQRWEAKVSTKLKNTTKQQAWPLIKDFFNIHKRFPNLATCYGVHGSNGEVGCIRYCAGSSLPSSQEVSWSKERLVAVDDIDLSIKYEIVECNIGFECYESTMRVVDGDGDGGCVVEWFFGVDPVDGLEFEELCEKQEFSSLIKGLKPGVTTSLALFN