AlfalfaGEDB | MS.gene53620

M. sativa cultivar XinJiangDaYe / MS.gene53620

KEGG: -
GO: -
PFAM: PF10604.12

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53620.t1	XP_013467174.1	87.8	172	18	2	1	170	1	171	1.10E-77	299.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53620.t1	P59082	36.2	152	92	2	8	159	19	165	2.9e-21	103.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53620.t1	I3SDX8	87.8	172	18	2	1	170	1	171	8.3e-78	299.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene53620	MS.gene40267	PPI
MS.gene53620	MS.gene014620	PPI
MS.gene53620	MS.gene72033	PPI
MS.gene53620	MS.gene40717	PPI
MS.gene53620	MS.gene40716	PPI
MS.gene53620	MS.gene40272	PPI
MS.gene53620	MS.gene014621	PPI
MS.gene53620	MS.gene40715	PPI
MS.gene40266	MS.gene53620	PPI
MS.gene72034	MS.gene53620	PPI
MS.gene014619	MS.gene53620	PPI
MS.gene72031	MS.gene53620	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53620.t1	MTR_1g046660	92.442	172	10	2	1	170	1	171	9.07e-107	302
MS.gene53620.t1	MTR_6g088445	37.107	159	94	2	7	163	8	162	8.07e-39	130
MS.gene53620.t1	MTR_6g088475	38.788	165	92	3	4	163	2	162	5.00e-38	128
MS.gene53620.t1	MTR_3g109880	35.976	164	96	3	9	164	8	170	5.77e-36	123
MS.gene53620.t1	MTR_5g081780	27.660	141	96	3	25	164	24	159	1.54e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53620.t1	AT2G25770	50.311	161	76	2	1	160	1	158	4.13e-55	171
MS.gene53620.t1	AT2G25770	50.311	161	76	2	1	160	1	158	4.13e-55	171
MS.gene53620.t1	AT4G32870	37.342	158	95	2	1	158	1	154	2.20e-32	113

Find 57 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGGTGGGGATGGTGGGTG+TGG	0.212530	1.2:-29423667	MS.gene53620:CDS
TCTAGCTACATTTCATTCTT+AGG	0.220042	1.2:+29423520	None:intergenic
TGTGGGGTTGAGTGGTTCTT+TGG	0.230418	1.2:-29423649	MS.gene53620:CDS
TCAAGTTATGGCTCAGAAAA+TGG	0.286347	1.2:-29423566	MS.gene53620:CDS
CCGTAACACGTGGCGAGGTT+AGG	0.314638	1.2:+29423895	None:intergenic
GTGGTGGGGATGGTGGGTGT+GGG	0.316554	1.2:-29423666	MS.gene53620:CDS
GAGATCGTGGAGTGTAATAT+TGG	0.324295	1.2:-29423733	MS.gene53620:CDS
TTTGGTGTTGATCCTGTTGA+TGG	0.333130	1.2:-29423631	MS.gene53620:CDS
AGTGATGGTGGTGGGGATGG+TGG	0.341362	1.2:-29423673	MS.gene53620:CDS
ATGGTGGGTGTGGGGTTGAG+TGG	0.347376	1.2:-29423657	MS.gene53620:CDS
TGGTGGGGATGGTGGGTGTG+GGG	0.350414	1.2:-29423665	MS.gene53620:CDS
CCTAACCTCGCCACGTGTTA+CGG	0.370350	1.2:-29423895	MS.gene53620:CDS
TCATGTTGGGCTTCAAGTTA+TGG	0.380134	1.2:-29423578	MS.gene53620:CDS
GTGATGGTGGTGGGGATGGT+GGG	0.389066	1.2:-29423672	MS.gene53620:CDS
CGTAACACGTGGCGAGGTTA+GGG	0.390712	1.2:+29423896	None:intergenic
CGAGGTTAGGGAAGCGTTTG+TGG	0.395604	1.2:+29423908	None:intergenic
GATAGTGATGGTGGTGGGGA+TGG	0.398633	1.2:-29423676	MS.gene53620:CDS
TGTTGATGGGTTGGAGTTTG+AGG	0.421293	1.2:-29423617	MS.gene53620:CDS
TGGGTTGGAGTTTGAGGAGT+TGG	0.424917	1.2:-29423611	MS.gene53620:CDS
TTGTTGATAGTGATGGTGGT+GGG	0.428464	1.2:-29423681	MS.gene53620:CDS
AAAACATTATTCAACAAAGA+TGG	0.432233	1.2:-29424008	MS.gene53620:CDS
TCAAACTCCAACCCATCAAC+AGG	0.436235	1.2:+29423619	None:intergenic
AGATCGTGGAGTGTAATATT+GGG	0.439079	1.2:-29423732	MS.gene53620:CDS
TGTTGATAGTGATGGTGGTG+GGG	0.451030	1.2:-29423680	MS.gene53620:CDS
ACTTCTTGTGATCCGTCTGA+TGG	0.451134	1.2:+29423814	None:intergenic
GAGCTGGTCAAAGGAGAGAT+TGG	0.472491	1.2:-29423791	MS.gene53620:CDS
TGTTGATCCTGTTGATGGGT+TGG	0.478872	1.2:-29423626	MS.gene53620:CDS
CTTCTTGTGATCCGTCTGAT+GGG	0.517003	1.2:+29423815	None:intergenic
TTGGTGTTGATCCTGTTGAT+GGG	0.518074	1.2:-29423630	MS.gene53620:CDS
TGGTGAATAAGTATCATGTT+GGG	0.521516	1.2:-29423591	MS.gene53620:CDS
GGATGCATACGTTACTGTGC+TGG	0.522809	1.2:-29423847	MS.gene53620:CDS
GTTGTTGATAGTGATGGTGG+TGG	0.524801	1.2:-29423682	MS.gene53620:CDS
AAACATTATTCAACAAAGAT+GGG	0.536088	1.2:-29424007	MS.gene53620:CDS
TTGTGATCCGTCTGATGGGA+GGG	0.539259	1.2:+29423819	None:intergenic
ACACCACAAAACAACAAGCA+TGG	0.539590	1.2:-29423957	MS.gene53620:CDS
CTACCTCACCGTTGGATCCG+TGG	0.543440	1.2:+29423869	None:intergenic
TTGGTGAATAAGTATCATGT+TGG	0.546383	1.2:-29423592	MS.gene53620:CDS
TACGGTATCCACGGATCCAA+CGG	0.553320	1.2:-29423877	MS.gene53620:CDS
TCAACAAAGATGGGAAGCCA+AGG	0.555814	1.2:-29423998	MS.gene53620:CDS
GGATTCTCCCTCCCATCAGA+CGG	0.569411	1.2:-29423826	MS.gene53620:CDS
AGGGTTGTTGATAGTGATGG+TGG	0.577449	1.2:-29423685	MS.gene53620:CDS
ACGGATCACAAGAAGTGAGC+TGG	0.577889	1.2:-29423807	MS.gene53620:CDS
CTTGTGATCCGTCTGATGGG+AGG	0.584237	1.2:+29423818	None:intergenic
TATGCATCCTACCTCACCGT+TGG	0.586722	1.2:+29423861	None:intergenic
ATGAGGGTTGTTGATAGTGA+TGG	0.587707	1.2:-29423688	MS.gene53620:CDS
TTGAAGAAGTCTTTGATGAG+TGG	0.592101	1.2:+29423934	None:intergenic
ACAAGAAGTGAGCTGGTCAA+AGG	0.594280	1.2:-29423800	MS.gene53620:CDS
ACTCAAGTCAACTTCATCAA+CGG	0.613369	1.2:+29423765	None:intergenic
CACGGATCCAACGGTGAGGT+AGG	0.622083	1.2:-29423868	MS.gene53620:CDS
GCCACGTGTTACGGTATCCA+CGG	0.641652	1.2:-29423886	MS.gene53620:CDS
AGTGTTATGAGTCAACTATG+AGG	0.643248	1.2:-29423705	MS.gene53620:CDS
GTGTTATGAGTCAACTATGA+GGG	0.655476	1.2:-29423704	MS.gene53620:CDS
GAGTATTAAGTATGAGATCG+TGG	0.664792	1.2:-29423746	MS.gene53620:CDS
AGTTATGGCTCAGAAAATGG+AGG	0.665948	1.2:-29423563	MS.gene53620:CDS
GGATACCGTAACACGTGGCG+AGG	0.690516	1.2:+29423890	None:intergenic
TCCGTGGATACCGTAACACG+TGG	0.704216	1.2:+29423885	None:intergenic
TATCCACGGATCCAACGGTG+AGG	0.759312	1.2:-29423872	MS.gene53620:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACATTATTCAACAAAGA+TGG	-	chr1.2:29423532-29423551	MS.gene53620:CDS	20.0%
!!	AAACATTATTCAACAAAGAT+GGG	-	chr1.2:29423533-29423552	MS.gene53620:CDS	20.0%
	TGGTGAATAAGTATCATGTT+GGG	-	chr1.2:29423949-29423968	MS.gene53620:CDS	30.0%
	TTGGTGAATAAGTATCATGT+TGG	-	chr1.2:29423948-29423967	MS.gene53620:CDS	30.0%
!!!	TTTGAGTTTTGTTGAAACCT+TGG	+	chr1.2:29423562-29423581	None:intergenic	30.0%
	ACTCAAGTCAACTTCATCAA+CGG	+	chr1.2:29423778-29423797	None:intergenic	35.0%
	AGATCGTGGAGTGTAATATT+GGG	-	chr1.2:29423808-29423827	MS.gene53620:CDS	35.0%
	AGTGTTATGAGTCAACTATG+AGG	-	chr1.2:29423835-29423854	MS.gene53620:CDS	35.0%
	GAGTATTAAGTATGAGATCG+TGG	-	chr1.2:29423794-29423813	MS.gene53620:CDS	35.0%
	GTGTTATGAGTCAACTATGA+GGG	-	chr1.2:29423836-29423855	MS.gene53620:CDS	35.0%
	TCAAGTTATGGCTCAGAAAA+TGG	-	chr1.2:29423974-29423993	MS.gene53620:CDS	35.0%
	TTGAAGAAGTCTTTGATGAG+TGG	+	chr1.2:29423609-29423628	None:intergenic	35.0%
	ACACCACAAAACAACAAGCA+TGG	-	chr1.2:29423583-29423602	MS.gene53620:CDS	40.0%
	AGTTATGGCTCAGAAAATGG+AGG	-	chr1.2:29423977-29423996	MS.gene53620:CDS	40.0%
	GAGATCGTGGAGTGTAATAT+TGG	-	chr1.2:29423807-29423826	MS.gene53620:CDS	40.0%
!	TCATGTTGGGCTTCAAGTTA+TGG	-	chr1.2:29423962-29423981	MS.gene53620:CDS	40.0%
!!	ATGAGGGTTGTTGATAGTGA+TGG	-	chr1.2:29423852-29423871	MS.gene53620:CDS	40.0%
!!	TTGGTGTTGATCCTGTTGAT+GGG	-	chr1.2:29423910-29423929	MS.gene53620:CDS	40.0%
!!	TTGTTGATAGTGATGGTGGT+GGG	-	chr1.2:29423859-29423878	MS.gene53620:CDS	40.0%
!!	TTTGGTGTTGATCCTGTTGA+TGG	-	chr1.2:29423909-29423928	MS.gene53620:CDS	40.0%
	ACAAGAAGTGAGCTGGTCAA+AGG	-	chr1.2:29423740-29423759	MS.gene53620:CDS	45.0%
	ACTTCTTGTGATCCGTCTGA+TGG	+	chr1.2:29423729-29423748	None:intergenic	45.0%
	CTTCTTGTGATCCGTCTGAT+GGG	+	chr1.2:29423728-29423747	None:intergenic	45.0%
	TCAAACTCCAACCCATCAAC+AGG	+	chr1.2:29423924-29423943	None:intergenic	45.0%
	TCAACAAAGATGGGAAGCCA+AGG	-	chr1.2:29423542-29423561	MS.gene53620:CDS	45.0%
!	TGTTGATCCTGTTGATGGGT+TGG	-	chr1.2:29423914-29423933	MS.gene53620:CDS	45.0%
!!	AGGGTTGTTGATAGTGATGG+TGG	-	chr1.2:29423855-29423874	MS.gene53620:CDS	45.0%
!!	GTTGTTGATAGTGATGGTGG+TGG	-	chr1.2:29423858-29423877	MS.gene53620:CDS	45.0%
!!	TGTTGATAGTGATGGTGGTG+GGG	-	chr1.2:29423860-29423879	MS.gene53620:CDS	45.0%
!!	TGTTGATGGGTTGGAGTTTG+AGG	-	chr1.2:29423923-29423942	MS.gene53620:CDS	45.0%
!!!	TGGCCATGCTTGTTGTTTTG+TGG	+	chr1.2:29423589-29423608	None:intergenic	45.0%
	ACGGATCACAAGAAGTGAGC+TGG	-	chr1.2:29423733-29423752	MS.gene53620:CDS	50.0%
	GAGCTGGTCAAAGGAGAGAT+TGG	-	chr1.2:29423749-29423768	MS.gene53620:CDS	50.0%
	GGATGCATACGTTACTGTGC+TGG	-	chr1.2:29423693-29423712	MS.gene53620:CDS	50.0%
	TACGGTATCCACGGATCCAA+CGG	-	chr1.2:29423663-29423682	MS.gene53620:CDS	50.0%
	TATGCATCCTACCTCACCGT+TGG	+	chr1.2:29423682-29423701	None:intergenic	50.0%
	TGTGGGGTTGAGTGGTTCTT+TGG	-	chr1.2:29423891-29423910	MS.gene53620:CDS	50.0%
	TTGTGATCCGTCTGATGGGA+GGG	+	chr1.2:29423724-29423743	None:intergenic	50.0%
!	TGGGTTGGAGTTTGAGGAGT+TGG	-	chr1.2:29423929-29423948	MS.gene53620:CDS	50.0%
	CCTAACCTCGCCACGTGTTA+CGG	-	chr1.2:29423645-29423664	MS.gene53620:CDS	55.0%
	CGTAACACGTGGCGAGGTTA+GGG	+	chr1.2:29423647-29423666	None:intergenic	55.0%
	CTTGTGATCCGTCTGATGGG+AGG	+	chr1.2:29423725-29423744	None:intergenic	55.0%
	GCCACGTGTTACGGTATCCA+CGG	-	chr1.2:29423654-29423673	MS.gene53620:CDS	55.0%
	GGATTCTCCCTCCCATCAGA+CGG	-	chr1.2:29423714-29423733	MS.gene53620:CDS	55.0%
	TATCCACGGATCCAACGGTG+AGG	-	chr1.2:29423668-29423687	MS.gene53620:CDS	55.0%
	TCCGTGGATACCGTAACACG+TGG	+	chr1.2:29423658-29423677	None:intergenic	55.0%
!!	CGAGGTTAGGGAAGCGTTTG+TGG	+	chr1.2:29423635-29423654	None:intergenic	55.0%
!!	GATAGTGATGGTGGTGGGGA+TGG	-	chr1.2:29423864-29423883	MS.gene53620:CDS	55.0%
	CACGGATCCAACGGTGAGGT+AGG	-	chr1.2:29423672-29423691	MS.gene53620:CDS	60.0%
	CCGTAACACGTGGCGAGGTT+AGG	+	chr1.2:29423648-29423667	None:intergenic	60.0%
	CTACCTCACCGTTGGATCCG+TGG	+	chr1.2:29423674-29423693	None:intergenic	60.0%
	GGATACCGTAACACGTGGCG+AGG	+	chr1.2:29423653-29423672	None:intergenic	60.0%
!!	AGTGATGGTGGTGGGGATGG+TGG	-	chr1.2:29423867-29423886	MS.gene53620:CDS	60.0%
!!	ATGGTGGGTGTGGGGTTGAG+TGG	-	chr1.2:29423883-29423902	MS.gene53620:CDS	60.0%
!!	GTGATGGTGGTGGGGATGGT+GGG	-	chr1.2:29423868-29423887	MS.gene53620:CDS	60.0%
!!	GTGGTGGGGATGGTGGGTGT+GGG	-	chr1.2:29423874-29423893	MS.gene53620:CDS	65.0%
!!	TGGTGGGGATGGTGGGTGTG+GGG	-	chr1.2:29423875-29423894	MS.gene53620:CDS	65.0%
!!	GGTGGTGGGGATGGTGGGTG+TGG	-	chr1.2:29423873-29423892	MS.gene53620:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	29423525	29424037	29423525	ID=MS.gene53620
chr1.2	mRNA	29423525	29424037	29423525	ID=MS.gene53620.t1;Parent=MS.gene53620
chr1.2	exon	29423525	29424037	29423525	ID=MS.gene53620.t1.exon1;Parent=MS.gene53620.t1
chr1.2	CDS	29423525	29424037	29423525	ID=cds.MS.gene53620.t1;Parent=MS.gene53620.t1

Gene Sequence

>MS.gene53620

ATGGAACAAAACATTATTCAACAAAGATGGGAAGCCAAGGTTTCAACAAAACTCAAAAACACCACAAAACAACAAGCATGGCCACTCATCAAAGACTTCTTCAATCTCCACAAACGCTTCCCTAACCTCGCCACGTGTTACGGTATCCACGGATCCAACGGTGAGGTAGGATGCATACGTTACTGTGCTGGATTCTCCCTCCCATCAGACGGATCACAAGAAGTGAGCTGGTCAAAGGAGAGATTGGTAGCCGTTGATGAAGTTGACTTGAGTATTAAGTATGAGATCGTGGAGTGTAATATTGGGTTTGAGTGTTATGAGTCAACTATGAGGGTTGTTGATAGTGATGGTGGTGGGGATGGTGGGTGTGGGGTTGAGTGGTTCTTTGGTGTTGATCCTGTTGATGGGTTGGAGTTTGAGGAGTTGGTGAATAAGTATCATGTTGGGCTTCAAGTTATGGCTCAGAAAATGGAGGAAGAGATTGCATCTTCACTTCCTAAGAATGAAATGTAG

Protein sequence

>MS.gene53620.t1

MEQNIIQQRWEAKVSTKLKNTTKQQAWPLIKDFFNLHKRFPNLATCYGIHGSNGEVGCIRYCAGFSLPSDGSQEVSWSKERLVAVDEVDLSIKYEIVECNIGFECYESTMRVVDSDGGGDGGCGVEWFFGVDPVDGLEFEELVNKYHVGLQVMAQKMEEEIASSLPKNEM