AlfalfaGEDB | MS.gene064976

M. sativa cultivar XinJiangDaYe / MS.gene064976

KEGG: -
GO: -
PFAM: PF10604.12

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064976.t1	XP_013467174.1	93	171	8	2	1	167	1	171	2.10E-76	295

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064976.t1	P59082	36.6	153	94	2	4	156	16	165	3.4e-22	106.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064976.t1	I3SDX8	93.0	171	8	2	1	167	1	171	1.5e-76	295.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene72033	MS.gene064976	PPI
MS.gene40272	MS.gene064976	PPI
MS.gene40267	MS.gene064976	PPI
MS.gene014621	MS.gene064976	PPI
MS.gene40717	MS.gene064976	PPI
MS.gene40266	MS.gene064976	PPI
MS.gene014620	MS.gene064976	PPI
MS.gene72034	MS.gene064976	PPI
MS.gene72031	MS.gene064976	PPI
MS.gene40716	MS.gene064976	PPI
MS.gene40715	MS.gene064976	PPI
MS.gene014619	MS.gene064976	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064976.t1	MTR_1g046660	93.567	171	7	2	1	167	1	171	1.91e-104	296
MS.gene064976.t1	MTR_3g109880	37.791	172	94	4	1	161	1	170	3.77e-38	129
MS.gene064976.t1	MTR_6g088445	35.802	162	96	2	3	160	5	162	5.13e-36	123
MS.gene064976.t1	MTR_6g088475	36.420	162	95	2	3	160	5	162	5.64e-35	120
MS.gene064976.t1	MTR_5g081780	26.619	139	98	2	24	161	24	159	3.07e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064976.t1	AT2G25770	50.000	154	71	2	7	157	8	158	1.11e-51	163
MS.gene064976.t1	AT2G25770	50.000	154	71	2	7	157	8	158	1.11e-51	163
MS.gene064976.t1	AT4G32870	39.597	149	86	3	8	155	9	154	5.09e-31	110

Find 56 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGATGGGGATGGTGGGTG+TGG	0.223284	1.4:-31239512	MS.gene064976:CDS
TGTGGGGTTGAGTGGTTCTT+TGG	0.230418	1.4:-31239494	MS.gene064976:CDS
TCAAGTTATGGCTCAGAAAA+TGG	0.286347	1.4:-31239411	MS.gene064976:CDS
GAGATTGTTGAGTGTAATAT+TGG	0.313988	1.4:-31239578	MS.gene064976:CDS
CGTAACACGTGGCGAGGTTT+GGG	0.334834	1.4:+31239735	None:intergenic
GTGATGGGGATGGTGGGTGT+GGG	0.338627	1.4:-31239511	MS.gene064976:CDS
CCGTAACACGTGGCGAGGTT+TGG	0.344196	1.4:+31239734	None:intergenic
ATGGTGGGTGTGGGGTTGAG+TGG	0.347376	1.4:-31239502	MS.gene064976:CDS
TTTCAGTTTGGTTGAAACTT+TGG	0.358456	1.4:+31239820	None:intergenic
GGTGATGGTGATGGGGATGG+TGG	0.372095	1.4:-31239518	MS.gene064976:CDS
TGATGGGGATGGTGGGTGTG+GGG	0.372487	1.4:-31239510	MS.gene064976:CDS
TTGTTGATGGTGATGGTGAT+GGG	0.375171	1.4:-31239526	MS.gene064976:CDS
GTTGTTGATGGTGATGGTGA+TGG	0.385145	1.4:-31239527	MS.gene064976:CDS
CCAAACCTCGCCACGTGTTA+CGG	0.392203	1.4:-31239734	MS.gene064976:CDS
TCGTGTTGGGCTTCAAGTTA+TGG	0.394949	1.4:-31239423	MS.gene064976:CDS
TTTGGTGTTGATCCTGTTGA+AGG	0.395954	1.4:-31239476	MS.gene064976:CDS
TGTTGAAGGGTTGGAGTTTG+AGG	0.413260	1.4:-31239462	MS.gene064976:CDS
GAGTGGCCATGCTTGTTGTT+TGG	0.426216	1.4:+31239790	None:intergenic
CAGCTCACTTCTTGTGATGA+TGG	0.430118	1.4:+31239653	None:intergenic
GTGATGGTGATGGGGATGGT+GGG	0.432935	1.4:-31239517	MS.gene064976:CDS
GATGGTGATGGTGATGGGGA+TGG	0.434342	1.4:-31239521	MS.gene064976:CDS
TCAAACTCCAACCCTTCAAC+AGG	0.436235	1.4:+31239464	None:intergenic
AACAAAACATTATTCAAAGA+TGG	0.437627	1.4:-31239847	MS.gene064976:CDS
AGGGTTGGAGTTTGAGGAGT+TGG	0.460105	1.4:-31239456	MS.gene064976:CDS
TCTAGCTACATTTCATTGCT+AGG	0.467241	1.4:+31239365	None:intergenic
GAGCTGGTCAAAGGAGAGAT+TGG	0.472491	1.4:-31239636	MS.gene064976:CDS
TGTTGATGGTGATGGTGATG+GGG	0.477314	1.4:-31239525	MS.gene064976:CDS
AGCTCACTTCTTGTGATGAT+GGG	0.493172	1.4:+31239654	None:intergenic
TGTTGATCCTGTTGAAGGGT+TGG	0.507759	1.4:-31239471	MS.gene064976:CDS
TGGCCATGCTTGTTGTTTGG+TGG	0.508658	1.4:+31239793	None:intergenic
TGGTGAGTAAGTATCGTGTT+GGG	0.510095	1.4:-31239436	MS.gene064976:CDS
CACTTCTTGTGATGATGGGA+GGG	0.524394	1.4:+31239658	None:intergenic
AGATTGTTGAGTGTAATATT+GGG	0.525385	1.4:-31239577	MS.gene064976:CDS
TTGGTGTTGATCCTGTTGAA+GGG	0.529067	1.4:-31239475	MS.gene064976:CDS
ACAAAACATTATTCAAAGAT+GGG	0.531963	1.4:-31239846	MS.gene064976:CDS
TTGGTGAGTAAGTATCGTGT+TGG	0.536250	1.4:-31239437	MS.gene064976:CDS
ACACCACCAAACAACAAGCA+TGG	0.541886	1.4:-31239796	MS.gene064976:CDS
GGATGCATACGCTACTGTGC+AGG	0.554173	1.4:-31239686	MS.gene064976:CDS
TACGGTATACACGGATCCAA+CGG	0.568944	1.4:-31239716	MS.gene064976:CDS
ACACGGATCCAACGGTGAGG+TGG	0.572963	1.4:-31239708	MS.gene064976:CDS
CATCATCACAAGAAGTGAGC+TGG	0.573089	1.4:-31239652	MS.gene064976:CDS
TCAACTATGAGGGTTGTTGA+TGG	0.573221	1.4:-31239539	MS.gene064976:CDS
CACGGATCCAACGGTGAGGT+GGG	0.578903	1.4:-31239707	MS.gene064976:CDS
ATGAGGGTTGTTGATGGTGA+TGG	0.579949	1.4:-31239533	MS.gene064976:CDS
TCACTTCTTGTGATGATGGG+AGG	0.583329	1.4:+31239657	None:intergenic
TATGCATCCCACCTCACCGT+TGG	0.586048	1.4:+31239700	None:intergenic
ACAAGAAGTGAGCTGGTCAA+AGG	0.594280	1.4:-31239645	MS.gene064976:CDS
TTCTTGTGATGATGGGAGGG+AGG	0.627581	1.4:+31239661	None:intergenic
ACTCAAGTCGACTTCATCAA+CGG	0.634209	1.4:+31239610	None:intergenic
AGTGTTATGAGTCAACTATG+AGG	0.643248	1.4:-31239550	MS.gene064976:CDS
GTGTTATGAGTCAACTATGA+GGG	0.655476	1.4:-31239549	MS.gene064976:CDS
AGTTATGGCTCAGAAAATGG+AGG	0.665948	1.4:-31239408	MS.gene064976:CDS
GCCACGTGTTACGGTATACA+CGG	0.713719	1.4:-31239725	MS.gene064976:CDS
GTATACCGTAACACGTGGCG+AGG	0.729321	1.4:+31239729	None:intergenic
TCCGTGTATACCGTAACACG+TGG	0.730775	1.4:+31239724	None:intergenic
TATACACGGATCCAACGGTG+AGG	0.764879	1.4:-31239711	MS.gene064976:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACAAAACATTATTCAAAGA+TGG	-	chr1.4:31239374-31239393	MS.gene064976:CDS	20.0%
!!	ACAAAACATTATTCAAAGAT+GGG	-	chr1.4:31239375-31239394	MS.gene064976:CDS	20.0%
!	AGATTGTTGAGTGTAATATT+GGG	-	chr1.4:31239644-31239663	MS.gene064976:CDS	25.0%
	GAGATTGTTGAGTGTAATAT+TGG	-	chr1.4:31239643-31239662	MS.gene064976:CDS	30.0%
	TTGAAAAAGTCTTTGATGAG+TGG	+	chr1.4:31239451-31239470	None:intergenic	30.0%
	TTTCAGTTTGGTTGAAACTT+TGG	+	chr1.4:31239404-31239423	None:intergenic	30.0%
	AGTGTTATGAGTCAACTATG+AGG	-	chr1.4:31239671-31239690	MS.gene064976:CDS	35.0%
	GTGTTATGAGTCAACTATGA+GGG	-	chr1.4:31239672-31239691	MS.gene064976:CDS	35.0%
	TCAAGTTATGGCTCAGAAAA+TGG	-	chr1.4:31239810-31239829	MS.gene064976:CDS	35.0%
!!!	TTTGGTGGTGTTTTTCAGTT+TGG	+	chr1.4:31239416-31239435	None:intergenic	35.0%
	AGCTCACTTCTTGTGATGAT+GGG	+	chr1.4:31239570-31239589	None:intergenic	40.0%
	AGTTATGGCTCAGAAAATGG+AGG	-	chr1.4:31239813-31239832	MS.gene064976:CDS	40.0%
	TCAACTATGAGGGTTGTTGA+TGG	-	chr1.4:31239682-31239701	MS.gene064976:CDS	40.0%
!	ACTCAAGTCGACTTCATCAA+CGG	+	chr1.4:31239614-31239633	None:intergenic	40.0%
!	TGGTGAGTAAGTATCGTGTT+GGG	-	chr1.4:31239785-31239804	MS.gene064976:CDS	40.0%
!	TTGGTGAGTAAGTATCGTGT+TGG	-	chr1.4:31239784-31239803	MS.gene064976:CDS	40.0%
!!	TTGGTGTTGATCCTGTTGAA+GGG	-	chr1.4:31239746-31239765	MS.gene064976:CDS	40.0%
!!	TTGTTGATGGTGATGGTGAT+GGG	-	chr1.4:31239695-31239714	MS.gene064976:CDS	40.0%
!!	TTTGGTGTTGATCCTGTTGA+AGG	-	chr1.4:31239745-31239764	MS.gene064976:CDS	40.0%
	ACAAGAAGTGAGCTGGTCAA+AGG	-	chr1.4:31239576-31239595	MS.gene064976:CDS	45.0%
	ACACCACCAAACAACAAGCA+TGG	-	chr1.4:31239425-31239444	MS.gene064976:CDS	45.0%
	CAGCTCACTTCTTGTGATGA+TGG	+	chr1.4:31239571-31239590	None:intergenic	45.0%
	CATCATCACAAGAAGTGAGC+TGG	-	chr1.4:31239569-31239588	MS.gene064976:CDS	45.0%
	TACGGTATACACGGATCCAA+CGG	-	chr1.4:31239505-31239524	MS.gene064976:CDS	45.0%
	TCAAACTCCAACCCTTCAAC+AGG	+	chr1.4:31239760-31239779	None:intergenic	45.0%
	TGTTGAAGGGTTGGAGTTTG+AGG	-	chr1.4:31239759-31239778	MS.gene064976:CDS	45.0%
	TGTTGATCCTGTTGAAGGGT+TGG	-	chr1.4:31239750-31239769	MS.gene064976:CDS	45.0%
!	TCGTGTTGGGCTTCAAGTTA+TGG	-	chr1.4:31239798-31239817	MS.gene064976:CDS	45.0%
!!	ATGAGGGTTGTTGATGGTGA+TGG	-	chr1.4:31239688-31239707	MS.gene064976:CDS	45.0%
!!	CACTTCTTGTGATGATGGGA+GGG	+	chr1.4:31239566-31239585	None:intergenic	45.0%
!!	GTTGTTGATGGTGATGGTGA+TGG	-	chr1.4:31239694-31239713	MS.gene064976:CDS	45.0%
!!	TCACTTCTTGTGATGATGGG+AGG	+	chr1.4:31239567-31239586	None:intergenic	45.0%
!!	TGTTGATGGTGATGGTGATG+GGG	-	chr1.4:31239696-31239715	MS.gene064976:CDS	45.0%
	AGGGTTGGAGTTTGAGGAGT+TGG	-	chr1.4:31239765-31239784	MS.gene064976:CDS	50.0%
	GAGCTGGTCAAAGGAGAGAT+TGG	-	chr1.4:31239585-31239604	MS.gene064976:CDS	50.0%
	GCCACGTGTTACGGTATACA+CGG	-	chr1.4:31239496-31239515	MS.gene064976:CDS	50.0%
	TATACACGGATCCAACGGTG+AGG	-	chr1.4:31239510-31239529	MS.gene064976:CDS	50.0%
	TCCGTGTATACCGTAACACG+TGG	+	chr1.4:31239500-31239519	None:intergenic	50.0%
	TGTGGGGTTGAGTGGTTCTT+TGG	-	chr1.4:31239727-31239746	MS.gene064976:CDS	50.0%
!	CGAGGTTTGGGAAGCTTTTG+TGG	+	chr1.4:31239477-31239496	None:intergenic	50.0%
!!	GAGTGGCCATGCTTGTTGTT+TGG	+	chr1.4:31239434-31239453	None:intergenic	50.0%
!!	TGGCCATGCTTGTTGTTTGG+TGG	+	chr1.4:31239431-31239450	None:intergenic	50.0%
!!	TTCTTGTGATGATGGGAGGG+AGG	+	chr1.4:31239563-31239582	None:intergenic	50.0%
	CCAAACCTCGCCACGTGTTA+CGG	-	chr1.4:31239487-31239506	MS.gene064976:CDS	55.0%
	CGTAACACGTGGCGAGGTTT+GGG	+	chr1.4:31239489-31239508	None:intergenic	55.0%
	GGATGCATACGCTACTGTGC+AGG	-	chr1.4:31239535-31239554	MS.gene064976:CDS	55.0%
	GTATACCGTAACACGTGGCG+AGG	+	chr1.4:31239495-31239514	None:intergenic	55.0%
	TATGCATCCCACCTCACCGT+TGG	+	chr1.4:31239524-31239543	None:intergenic	55.0%
!!	GATGGTGATGGTGATGGGGA+TGG	-	chr1.4:31239700-31239719	MS.gene064976:CDS	55.0%
!!	GTGATGGTGATGGGGATGGT+GGG	-	chr1.4:31239704-31239723	MS.gene064976:CDS	55.0%
	ACACGGATCCAACGGTGAGG+TGG	-	chr1.4:31239513-31239532	MS.gene064976:CDS	60.0%
	CACGGATCCAACGGTGAGGT+GGG	-	chr1.4:31239514-31239533	MS.gene064976:CDS	60.0%
	CCGTAACACGTGGCGAGGTT+TGG	+	chr1.4:31239490-31239509	None:intergenic	60.0%
!!	ATGGTGGGTGTGGGGTTGAG+TGG	-	chr1.4:31239719-31239738	MS.gene064976:CDS	60.0%
!!	GGTGATGGTGATGGGGATGG+TGG	-	chr1.4:31239703-31239722	MS.gene064976:CDS	60.0%
!!	GTGATGGGGATGGTGGGTGT+GGG	-	chr1.4:31239710-31239729	MS.gene064976:CDS	60.0%
!!	TGATGGGGATGGTGGGTGTG+GGG	-	chr1.4:31239711-31239730	MS.gene064976:CDS	60.0%
!!	GGTGATGGGGATGGTGGGTG+TGG	-	chr1.4:31239709-31239728	MS.gene064976:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	31239370	31239873	31239370	ID=MS.gene064976
chr1.4	mRNA	31239370	31239873	31239370	ID=MS.gene064976.t1;Parent=MS.gene064976
chr1.4	exon	31239370	31239873	31239370	ID=MS.gene064976.t1.exon1;Parent=MS.gene064976.t1
chr1.4	CDS	31239370	31239873	31239370	ID=cds.MS.gene064976.t1;Parent=MS.gene064976.t1

Gene Sequence

>MS.gene064976

ATGGAACAAAACATTATTCAAAGATGGGAAGCCAAAGTTTCAACCAAACTGAAAAACACCACCAAACAACAAGCATGGCCACTCATCAAAGACTTTTTCAATCTCCACAAAAGCTTCCCAAACCTCGCCACGTGTTACGGTATACACGGATCCAACGGTGAGGTGGGATGCATACGCTACTGTGCAGGATCCTCCCTCCCATCATCACAAGAAGTGAGCTGGTCAAAGGAGAGATTGGTAGCCGTTGATGAAGTCGACTTGAGTATTAAGTATGAGATTGTTGAGTGTAATATTGGGTTTGAGTGTTATGAGTCAACTATGAGGGTTGTTGATGGTGATGGTGATGGGGATGGTGGGTGTGGGGTTGAGTGGTTCTTTGGTGTTGATCCTGTTGAAGGGTTGGAGTTTGAGGAGTTGGTGAGTAAGTATCGTGTTGGGCTTCAAGTTATGGCTCAGAAAATGGAGGAAGAGATTGCATCTTCACTTCCTAGCAATGAAATGTAG

Protein sequence

>MS.gene064976.t1

MEQNIIQRWEAKVSTKLKNTTKQQAWPLIKDFFNLHKSFPNLATCYGIHGSNGEVGCIRYCAGSSLPSSQEVSWSKERLVAVDEVDLSIKYEIVECNIGFECYESTMRVVDGDGDGDGGCGVEWFFGVDPVEGLEFEELVSKYRVGLQVMAQKMEEEIASSLPSNEM