Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014620.t1 | GAU14248.1 | 75.4 | 167 | 37 | 3 | 1 | 164 | 1 | 166 | 4.50E-69 | 270.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014620.t1 | A0A2K3NXU4 | 73.7 | 167 | 41 | 2 | 1 | 164 | 1 | 167 | 2.7e-68 | 267.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53620 | MS.gene014620 | PPI |
| MS.gene64289 | MS.gene014620 | PPI |
| MS.gene014620 | MS.gene064976 | PPI |
| MS.gene94983 | MS.gene014620 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014620.t1 | MTR_3g109880 | 60.335 | 179 | 55 | 5 | 1 | 169 | 1 | 173 | 5.68e-69 | 207 |
| MS.gene014620.t1 | MTR_6g088445 | 42.262 | 168 | 81 | 3 | 9 | 170 | 10 | 167 | 2.66e-38 | 129 |
| MS.gene014620.t1 | MTR_6g088475 | 43.038 | 158 | 74 | 3 | 9 | 160 | 10 | 157 | 4.41e-36 | 123 |
| MS.gene014620.t1 | MTR_1g046660 | 33.908 | 174 | 105 | 4 | 3 | 170 | 2 | 171 | 2.03e-24 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014620.t1 | AT4G32870 | 47.239 | 163 | 76 | 4 | 1 | 161 | 1 | 155 | 9.19e-49 | 155 |
| MS.gene014620.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 7.08e-31 | 110 |
| MS.gene014620.t1 | AT2G25770 | 36.242 | 149 | 81 | 4 | 1 | 144 | 1 | 140 | 7.08e-31 | 110 |
Find 54 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTGGAAACGTTATTA+AGG | 0.203448 | 3.1:-88246844 | MS.gene014620:CDS |
| TGAAATTGTTGATAACAGTA+TGG | 0.310256 | 3.1:-88247012 | MS.gene014620:CDS |
| AGGAAAATAAGCCAACAAAA+TGG | 0.322964 | 3.1:-88247274 | MS.gene014620:CDS |
| TTCATTGGAAACGTTATTAA+GGG | 0.346776 | 3.1:-88246843 | MS.gene014620:CDS |
| CAAGATTTGAATTCTTTCAT+TGG | 0.349097 | 3.1:-88246858 | MS.gene014620:CDS |
| TATAGTATGGCAAGTGTTAG+AGG | 0.403359 | 3.1:-88247210 | MS.gene014620:CDS |
| GGAAAATAAGCCAACAAAAT+GGG | 0.403599 | 3.1:-88247273 | MS.gene014620:CDS |
| TCTTTAACCCACTCAGAAGT+TGG | 0.415580 | 3.1:+88247077 | None:intergenic |
| ATGGATTGTTATAAGCTTGA+AGG | 0.449145 | 3.1:-88247158 | MS.gene014620:CDS |
| TCATCATCAACGCCGCTGAT+TGG | 0.462447 | 3.1:+88246948 | None:intergenic |
| TCAAGCTTATAACAATCCAT+TGG | 0.469517 | 3.1:+88247161 | None:intergenic |
| GGTGTTTGTGATCCGGTGGA+AGG | 0.474182 | 3.1:-88246891 | MS.gene014620:CDS |
| ACTGTTATCAACAATTTCAT+AGG | 0.476144 | 3.1:+88247016 | None:intergenic |
| TATAAGCTTGAAGGGATTGA+AGG | 0.477132 | 3.1:-88247149 | MS.gene014620:CDS |
| CAAGCTTATAACAATCCATT+GGG | 0.479296 | 3.1:+88247162 | None:intergenic |
| GAAATTGTTGATAACAGTAT+GGG | 0.485941 | 3.1:-88247011 | MS.gene014620:CDS |
| ATGGGAGGGTGTTTGTGATC+CGG | 0.489610 | 3.1:-88246898 | MS.gene014620:CDS |
| GAACTGAGGAGGATATAGTA+TGG | 0.492546 | 3.1:-88247223 | MS.gene014620:CDS |
| AACCCACTCAGAAGTTGGAT+TGG | 0.500294 | 3.1:+88247082 | None:intergenic |
| AAAGCCACCGCTGAGGTAAG+AGG | 0.507470 | 3.1:-88247245 | MS.gene014620:CDS |
| TGGATTGTTATAAGCTTGAA+GGG | 0.510009 | 3.1:-88247157 | MS.gene014620:CDS |
| ATAGGTCAAACAACGTTGGA+TGG | 0.513283 | 3.1:+88247034 | None:intergenic |
| TAGCTAGTCAAAGTTTGTGT+GGG | 0.515225 | 3.1:-88246788 | MS.gene014620:CDS |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.1:-88246917 | MS.gene014620:CDS |
| GTGTTTGTGATCCGGTGGAA+GGG | 0.541416 | 3.1:-88246890 | MS.gene014620:CDS |
| TGGAAACGTTATTAAGGGCA+TGG | 0.549534 | 3.1:-88246838 | MS.gene014620:CDS |
| TTTGTGATCCGGTGGAAGGG+TGG | 0.560676 | 3.1:-88246887 | MS.gene014620:CDS |
| TGAACTGTCCACCCTTCCAC+CGG | 0.560925 | 3.1:+88246879 | None:intergenic |
| GAAGGGATTGAAGGTCAACC+TGG | 0.564797 | 3.1:-88247140 | MS.gene014620:CDS |
| TCAACGCCGCTGATTGGCAA+TGG | 0.566877 | 3.1:+88246954 | None:intergenic |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.1:-88246916 | MS.gene014620:CDS |
| CCATTGGGAGCCACTTGTGT+AGG | 0.567437 | 3.1:+88247177 | None:intergenic |
| TTAGCTAGTCAAAGTTTGTG+TGG | 0.568989 | 3.1:-88246789 | MS.gene014620:CDS |
| AGTGAGATCAATAATGGGAG+AGG | 0.570998 | 3.1:-88247294 | None:intergenic |
| TAGGTCAAACAACGTTGGAT+GGG | 0.573010 | 3.1:+88247035 | None:intergenic |
| GCACAATAACGAACGAGACC+AGG | 0.573329 | 3.1:+88247122 | None:intergenic |
| AAATTGTTGATAACAGTATG+GGG | 0.598578 | 3.1:-88247010 | MS.gene014620:CDS |
| TTTCATAGGTCAAACAACGT+TGG | 0.600620 | 3.1:+88247030 | None:intergenic |
| TGCCAATCCAACTTCTGAGT+GGG | 0.606140 | 3.1:-88247084 | MS.gene014620:CDS |
| AGTTCCTCTTACCTCAGCGG+TGG | 0.616369 | 3.1:+88247241 | None:intergenic |
| CCTACACAAGTGGCTCCCAA+TGG | 0.622824 | 3.1:-88247177 | MS.gene014620:CDS |
| AATAAGCCAACAAAATGGGA+AGG | 0.626979 | 3.1:-88247269 | MS.gene014620:CDS |
| GGAGGGTGTTTGTGATCCGG+TGG | 0.630744 | 3.1:-88246895 | MS.gene014620:CDS |
| CGCTGAGGTAAGAGGAACTG+AGG | 0.632981 | 3.1:-88247237 | MS.gene014620:CDS |
| CTCAGTTCCTCTTACCTCAG+CGG | 0.641075 | 3.1:+88247238 | None:intergenic |
| AGGTTGTATGATCGAATGGG+AGG | 0.648114 | 3.1:-88246913 | MS.gene014620:CDS |
| GATGATGATGCTAAACGTGT+AGG | 0.650720 | 3.1:-88246933 | MS.gene014620:CDS |
| ATTTCTGTAACCTACACAAG+TGG | 0.658716 | 3.1:-88247187 | MS.gene014620:CDS |
| TGAGGTAAGAGGAACTGAGG+AGG | 0.667990 | 3.1:-88247234 | MS.gene014620:CDS |
| GGTTGTATGATCGAATGGGA+GGG | 0.676731 | 3.1:-88246912 | MS.gene014620:CDS |
| GTGCCAATCCAACTTCTGAG+TGG | 0.684174 | 3.1:-88247085 | MS.gene014620:CDS |
| GTATTGCCATTGCCAATCAG+CGG | 0.692711 | 3.1:-88246960 | MS.gene014620:CDS |
| TATGGGGTTTAAGAATTACG+TGG | 0.696481 | 3.1:-88246994 | MS.gene014620:CDS |
| GGAAGGTAAAGCCACCGCTG+AGG | 0.701441 | 3.1:-88247252 | MS.gene014620:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTGTTATCAACAATTTCAT+AGG | + | chr3.1:88247047-88247066 | None:intergenic | 25.0% |
| ! | CAAGATTTGAATTCTTTCAT+TGG | - | chr3.1:88247202-88247221 | MS.gene014620:CDS | 25.0% |
| ! | TATTGTGCTTTCACAAAAAA+GGG | - | chr3.1:88246953-88246972 | MS.gene014620:CDS | 25.0% |
| ! | TTATTGTGCTTTCACAAAAA+AGG | - | chr3.1:88246952-88246971 | MS.gene014620:CDS | 25.0% |
| ! | TTCATTGGAAACGTTATTAA+GGG | - | chr3.1:88247217-88247236 | MS.gene014620:CDS | 25.0% |
| ! | TTTCATTGGAAACGTTATTA+AGG | - | chr3.1:88247216-88247235 | MS.gene014620:CDS | 25.0% |
| !! | ATATGTAAGCTCAATTTTGT+TGG | + | chr3.1:88247248-88247267 | None:intergenic | 25.0% |
| !!! | AAATTGTTGATAACAGTATG+GGG | - | chr3.1:88247050-88247069 | MS.gene014620:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAGTAT+GGG | - | chr3.1:88247049-88247068 | MS.gene014620:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAGTA+TGG | - | chr3.1:88247048-88247067 | MS.gene014620:CDS | 25.0% |
| AGGAAAATAAGCCAACAAAA+TGG | - | chr3.1:88246786-88246805 | MS.gene014620:CDS | 30.0% | |
| CAAGCTTATAACAATCCATT+GGG | + | chr3.1:88246901-88246920 | None:intergenic | 30.0% | |
| GGAAAATAAGCCAACAAAAT+GGG | - | chr3.1:88246787-88246806 | MS.gene014620:CDS | 30.0% | |
| TCAAGCTTATAACAATCCAT+TGG | + | chr3.1:88246902-88246921 | None:intergenic | 30.0% | |
| TGGATTGTTATAAGCTTGAA+GGG | - | chr3.1:88246903-88246922 | MS.gene014620:CDS | 30.0% | |
| ! | ATGGATTGTTATAAGCTTGA+AGG | - | chr3.1:88246902-88246921 | MS.gene014620:CDS | 30.0% |
| AATAAGCCAACAAAATGGGA+AGG | - | chr3.1:88246791-88246810 | MS.gene014620:CDS | 35.0% | |
| ATTTCTGTAACCTACACAAG+TGG | - | chr3.1:88246873-88246892 | MS.gene014620:CDS | 35.0% | |
| TAGCTAGTCAAAGTTTGTGT+GGG | - | chr3.1:88247272-88247291 | MS.gene014620:CDS | 35.0% | |
| TATAAGCTTGAAGGGATTGA+AGG | - | chr3.1:88246911-88246930 | MS.gene014620:CDS | 35.0% | |
| TATAGTATGGCAAGTGTTAG+AGG | - | chr3.1:88246850-88246869 | MS.gene014620:CDS | 35.0% | |
| TATGGGGTTTAAGAATTACG+TGG | - | chr3.1:88247066-88247085 | MS.gene014620:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.1:88247144-88247163 | MS.gene014620:CDS | 35.0% | |
| TTAGCTAGTCAAAGTTTGTG+TGG | - | chr3.1:88247271-88247290 | MS.gene014620:CDS | 35.0% | |
| TTTCATAGGTCAAACAACGT+TGG | + | chr3.1:88247033-88247052 | None:intergenic | 35.0% | |
| ATAGGTCAAACAACGTTGGA+TGG | + | chr3.1:88247029-88247048 | None:intergenic | 40.0% | |
| GAACTGAGGAGGATATAGTA+TGG | - | chr3.1:88246837-88246856 | MS.gene014620:CDS | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.1:88247143-88247162 | MS.gene014620:CDS | 40.0% | |
| TAGGTCAAACAACGTTGGAT+GGG | + | chr3.1:88247028-88247047 | None:intergenic | 40.0% | |
| TCTTTAACCCACTCAGAAGT+TGG | + | chr3.1:88246986-88247005 | None:intergenic | 40.0% | |
| TGGAAACGTTATTAAGGGCA+TGG | - | chr3.1:88247222-88247241 | MS.gene014620:CDS | 40.0% | |
| ! | GATGATGATGCTAAACGTGT+AGG | - | chr3.1:88247127-88247146 | MS.gene014620:CDS | 40.0% |
| ! | GCTTTACCTTCCCATTTTGT+TGG | + | chr3.1:88246800-88246819 | None:intergenic | 40.0% |
| AACCCACTCAGAAGTTGGAT+TGG | + | chr3.1:88246981-88247000 | None:intergenic | 45.0% | |
| AGGTTGTATGATCGAATGGG+AGG | - | chr3.1:88247147-88247166 | MS.gene014620:CDS | 45.0% | |
| GGTTGTATGATCGAATGGGA+GGG | - | chr3.1:88247148-88247167 | MS.gene014620:CDS | 45.0% | |
| GTATTGCCATTGCCAATCAG+CGG | - | chr3.1:88247100-88247119 | MS.gene014620:CDS | 45.0% | |
| TGCCAATCCAACTTCTGAGT+GGG | - | chr3.1:88246976-88246995 | MS.gene014620:CDS | 45.0% | |
| CTCAGTTCCTCTTACCTCAG+CGG | + | chr3.1:88246825-88246844 | None:intergenic | 50.0% | |
| GAAGGGATTGAAGGTCAACC+TGG | - | chr3.1:88246920-88246939 | MS.gene014620:CDS | 50.0% | |
| GCACAATAACGAACGAGACC+AGG | + | chr3.1:88246941-88246960 | None:intergenic | 50.0% | |
| GTGCCAATCCAACTTCTGAG+TGG | - | chr3.1:88246975-88246994 | MS.gene014620:CDS | 50.0% | |
| GTGTTTGTGATCCGGTGGAA+GGG | - | chr3.1:88247170-88247189 | MS.gene014620:CDS | 50.0% | |
| TCATCATCAACGCCGCTGAT+TGG | + | chr3.1:88247115-88247134 | None:intergenic | 50.0% | |
| TGAGGTAAGAGGAACTGAGG+AGG | - | chr3.1:88246826-88246845 | MS.gene014620:CDS | 50.0% | |
| !! | ATGGGAGGGTGTTTGTGATC+CGG | - | chr3.1:88247162-88247181 | MS.gene014620:CDS | 50.0% |
| AAAGCCACCGCTGAGGTAAG+AGG | - | chr3.1:88246815-88246834 | MS.gene014620:CDS | 55.0% | |
| AGTTCCTCTTACCTCAGCGG+TGG | + | chr3.1:88246822-88246841 | None:intergenic | 55.0% | |
| CCATTGGGAGCCACTTGTGT+AGG | + | chr3.1:88246886-88246905 | None:intergenic | 55.0% | |
| CCTACACAAGTGGCTCCCAA+TGG | - | chr3.1:88246883-88246902 | MS.gene014620:CDS | 55.0% | |
| CGCTGAGGTAAGAGGAACTG+AGG | - | chr3.1:88246823-88246842 | MS.gene014620:CDS | 55.0% | |
| GGTGTTTGTGATCCGGTGGA+AGG | - | chr3.1:88247169-88247188 | MS.gene014620:CDS | 55.0% | |
| TGAACTGTCCACCCTTCCAC+CGG | + | chr3.1:88247184-88247203 | None:intergenic | 55.0% | |
| TTTGTGATCCGGTGGAAGGG+TGG | - | chr3.1:88247173-88247192 | MS.gene014620:CDS | 55.0% | |
| ! | TCAACGCCGCTGATTGGCAA+TGG | + | chr3.1:88247109-88247128 | None:intergenic | 55.0% |
| GGAAGGTAAAGCCACCGCTG+AGG | - | chr3.1:88246808-88246827 | MS.gene014620:CDS | 60.0% | |
| !! | GGAGGGTGTTTGTGATCCGG+TGG | - | chr3.1:88247165-88247184 | MS.gene014620:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 88246779 | 88247303 | 88246779 | ID=MS.gene014620 |
| chr3.1 | mRNA | 88246779 | 88247303 | 88246779 | ID=MS.gene014620.t1;Parent=MS.gene014620 |
| chr3.1 | exon | 88246779 | 88247303 | 88246779 | ID=MS.gene014620.t1.exon1;Parent=MS.gene014620.t1 |
| chr3.1 | CDS | 88246779 | 88247303 | 88246779 | ID=cds.MS.gene014620.t1;Parent=MS.gene014620.t1 |
| Gene Sequence |
| Protein sequence |
