Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014621.t1 | GAU14248.1 | 80 | 170 | 28 | 2 | 1 | 166 | 1 | 168 | 4.00E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014621.t1 | A0A392QMR0 | 79.7 | 172 | 27 | 3 | 1 | 166 | 1 | 170 | 2.5e-74 | 287.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64289 | MS.gene014621 | PPI |
| MS.gene014621 | MS.gene94983 | PPI |
| MS.gene014621 | MS.gene064976 | PPI |
| MS.gene53620 | MS.gene014621 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014621.t1 | MTR_3g109880 | 59.770 | 174 | 57 | 3 | 3 | 167 | 2 | 171 | 8.71e-67 | 201 |
| MS.gene014621.t1 | MTR_6g088445 | 45.122 | 164 | 76 | 3 | 8 | 166 | 9 | 163 | 4.02e-44 | 144 |
| MS.gene014621.t1 | MTR_6g088475 | 43.125 | 160 | 77 | 3 | 8 | 162 | 9 | 159 | 4.76e-40 | 133 |
| MS.gene014621.t1 | MTR_1g046660 | 35.802 | 162 | 94 | 4 | 4 | 160 | 3 | 159 | 4.75e-25 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014621.t1 | AT4G32870 | 45.062 | 162 | 81 | 3 | 1 | 161 | 1 | 155 | 4.55e-45 | 145 |
| MS.gene014621.t1 | AT2G25770 | 36.145 | 166 | 90 | 6 | 1 | 160 | 1 | 156 | 1.81e-29 | 106 |
| MS.gene014621.t1 | AT2G25770 | 36.145 | 166 | 90 | 6 | 1 | 160 | 1 | 156 | 1.81e-29 | 106 |
Find 38 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATACTTGTTATAAATTTGA+AGG | 0.346428 | 3.1:-88240212 | MS.gene014621:CDS |
| AATTTATAACAAGTATCAAT+TGG | 0.372630 | 3.1:+88240218 | None:intergenic |
| GAAAGTACTGCCACTGCAAA+TGG | 0.400092 | 3.1:-88240006 | MS.gene014621:CDS |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.1:+88240058 | None:intergenic |
| ATTGAGAAAGTTTCCAATGT+AGG | 0.404010 | 3.1:+88239902 | None:intergenic |
| TATAAATTTGAAGGAATTGA+AGG | 0.410358 | 3.1:-88240203 | MS.gene014621:CDS |
| TTGCCACACTATTTCCTCTT+CGG | 0.429431 | 3.1:+88240277 | None:intergenic |
| ATAGGACAAACAACGTTGTA+CGG | 0.455654 | 3.1:+88240076 | None:intergenic |
| CAAAATTTGAAATCCTACAT+TGG | 0.474889 | 3.1:-88239915 | MS.gene014621:CDS |
| ATTTATAACAAGTATCAATT+GGG | 0.476403 | 3.1:+88240219 | None:intergenic |
| TTCGGTTCCTCTTACCTCAA+CGG | 0.512935 | 3.1:+88240295 | None:intergenic |
| AAAGCCACCGTTGAGGTAAG+AGG | 0.518386 | 3.1:-88240302 | MS.gene014621:CDS |
| CACTCGGCAAGAATAATGGC+AGG | 0.521022 | 3.1:-88240353 | None:intergenic |
| GTGTAGGTTGTATGATCGAA+TGG | 0.523159 | 3.1:-88239962 | MS.gene014621:CDS |
| GAAGGAATTGAAGGCCAACC+TGG | 0.529028 | 3.1:-88240194 | MS.gene014621:CDS |
| CAATTGGGAGCCATTTGTGT+AGG | 0.537544 | 3.1:+88240234 | None:intergenic |
| TAGGACAAACAACGTTGTAC+GGG | 0.547037 | 3.1:+88240077 | None:intergenic |
| TGGAAACTTTCTCAATTGCA+TGG | 0.549994 | 3.1:-88239895 | MS.gene014621:CDS |
| TGTAGGTTGTATGATCGAAT+GGG | 0.567289 | 3.1:-88239961 | MS.gene014621:CDS |
| GAAATTGTTGATAACAACAT+GGG | 0.602430 | 3.1:-88240053 | MS.gene014621:CDS |
| AATGGGAGTCTGTGGAAGGG+TGG | 0.606114 | 3.1:-88239944 | MS.gene014621:CDS |
| CTCGGCAAGAATAATGGCAG+GGG | 0.612851 | 3.1:-88240351 | None:intergenic |
| ATCGAATGGGAGTCTGTGGA+AGG | 0.618429 | 3.1:-88239948 | MS.gene014621:CDS |
| TGAAATTGTTGATAACAACA+TGG | 0.627477 | 3.1:-88240054 | MS.gene014621:CDS |
| GGTGATGATGCTAAACGTGT+AGG | 0.644167 | 3.1:-88239978 | MS.gene014621:CDS |
| GTACTGCCACTGCAAATGGA+CGG | 0.644735 | 3.1:-88240002 | MS.gene014621:CDS |
| TATGATCGAATGGGAGTCTG+TGG | 0.645602 | 3.1:-88239952 | MS.gene014621:CDS |
| GCACAATAACGAATGAGACC+AGG | 0.650328 | 3.1:+88240176 | None:intergenic |
| CATGGGATTTAAGAATTACG+TGG | 0.650792 | 3.1:-88240036 | MS.gene014621:CDS |
| GGTTCCTCTTACCTCAACGG+TGG | 0.651251 | 3.1:+88240298 | None:intergenic |
| TCACCACCGTCCATTTGCAG+TGG | 0.660619 | 3.1:+88239996 | None:intergenic |
| ACTCGGCAAGAATAATGGCA+GGG | 0.664895 | 3.1:-88240352 | None:intergenic |
| TGAGGTAAGAGGAACCGAAG+AGG | 0.684493 | 3.1:-88240291 | MS.gene014621:CDS |
| TCGAATGGGAGTCTGTGGAA+GGG | 0.695670 | 3.1:-88239947 | MS.gene014621:CDS |
| GGAAGGTAAAGCCACCGTTG+AGG | 0.711159 | 3.1:-88240309 | MS.gene014621:CDS |
| AATAACGAATGAGACCAGGT+TGG | 0.715813 | 3.1:+88240180 | None:intergenic |
| GAACCGAAGAGGAAATAGTG+TGG | 0.729750 | 3.1:-88240280 | MS.gene014621:CDS |
| CTGCCACTGCAAATGGACGG+TGG | 0.775966 | 3.1:-88239999 | MS.gene014621:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATAACAAGTATCAAT+TGG | + | chr3.1:88239977-88239996 | None:intergenic | 15.0% |
| !! | ATTTATAACAAGTATCAATT+GGG | + | chr3.1:88239976-88239995 | None:intergenic | 15.0% |
| !! | TATAAATTTGAAGGAATTGA+AGG | - | chr3.1:88239989-88240008 | MS.gene014621:CDS | 20.0% |
| !!! | GATACTTGTTATAAATTTGA+AGG | - | chr3.1:88239980-88239999 | MS.gene014621:CDS | 20.0% |
| ! | GGAAAATAAGCTAAAAAGAT+GGG | - | chr3.1:88239862-88239881 | MS.gene014621:CDS | 25.0% |
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.1:88240137-88240156 | None:intergenic | 25.0% |
| !! | ATTTTTGTAACCTACACAAA+TGG | - | chr3.1:88239948-88239967 | MS.gene014621:CDS | 25.0% |
| !! | CAAAATTTGAAATCCTACAT+TGG | - | chr3.1:88240277-88240296 | MS.gene014621:CDS | 25.0% |
| !! | TCTTTGAAAAACTTAACAAC+TGG | + | chr3.1:88240076-88240095 | None:intergenic | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.1:88240139-88240158 | MS.gene014621:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.1:88240138-88240157 | MS.gene014621:CDS | 25.0% |
| !!! | TTCATCAACACTTTTTTTTG+TGG | + | chr3.1:88240044-88240063 | None:intergenic | 25.0% |
| AATAAGCTAAAAAGATGGGA+AGG | - | chr3.1:88239866-88239885 | MS.gene014621:CDS | 30.0% | |
| ATTGAGAAAGTTTCCAATGT+AGG | + | chr3.1:88240293-88240312 | None:intergenic | 30.0% | |
| GGGAAAATAAGCTAAAAAGA+TGG | - | chr3.1:88239861-88239880 | MS.gene014621:CDS | 30.0% | |
| ! | ATATGCAAGCTCAATGTTTT+TGG | + | chr3.1:88240323-88240342 | None:intergenic | 30.0% |
| ATAGGACAAACAACGTTGTA+CGG | + | chr3.1:88240119-88240138 | None:intergenic | 35.0% | |
| CATGGGATTTAAGAATTACG+TGG | - | chr3.1:88240156-88240175 | MS.gene014621:CDS | 35.0% | |
| TGGAAACTTTCTCAATTGCA+TGG | - | chr3.1:88240297-88240316 | MS.gene014621:CDS | 35.0% | |
| TGTAGGTTGTATGATCGAAT+GGG | - | chr3.1:88240231-88240250 | MS.gene014621:CDS | 35.0% | |
| AATAACGAATGAGACCAGGT+TGG | + | chr3.1:88240015-88240034 | None:intergenic | 40.0% | |
| GTGTAGGTTGTATGATCGAA+TGG | - | chr3.1:88240230-88240249 | MS.gene014621:CDS | 40.0% | |
| TAGGACAAACAACGTTGTAC+GGG | + | chr3.1:88240118-88240137 | None:intergenic | 40.0% | |
| TTGCCACACTATTTCCTCTT+CGG | + | chr3.1:88239918-88239937 | None:intergenic | 40.0% | |
| CAATTGGGAGCCATTTGTGT+AGG | + | chr3.1:88239961-88239980 | None:intergenic | 45.0% | |
| GAACCGAAGAGGAAATAGTG+TGG | - | chr3.1:88239912-88239931 | MS.gene014621:CDS | 45.0% | |
| GCACAATAACGAATGAGACC+AGG | + | chr3.1:88240019-88240038 | None:intergenic | 45.0% | |
| TATGATCGAATGGGAGTCTG+TGG | - | chr3.1:88240240-88240259 | MS.gene014621:CDS | 45.0% | |
| TTCGGTTCCTCTTACCTCAA+CGG | + | chr3.1:88239900-88239919 | None:intergenic | 45.0% | |
| ! | GGTGATGATGCTAAACGTGT+AGG | - | chr3.1:88240214-88240233 | MS.gene014621:CDS | 45.0% |
| !! | GAAAGTACTGCCACTGCAAA+TGG | - | chr3.1:88240186-88240205 | MS.gene014621:CDS | 45.0% |
| AAAGCCACCGTTGAGGTAAG+AGG | - | chr3.1:88239890-88239909 | MS.gene014621:CDS | 50.0% | |
| ATCGAATGGGAGTCTGTGGA+AGG | - | chr3.1:88240244-88240263 | MS.gene014621:CDS | 50.0% | |
| GAAGGAATTGAAGGCCAACC+TGG | - | chr3.1:88239998-88240017 | MS.gene014621:CDS | 50.0% | |
| GTACTGCCACTGCAAATGGA+CGG | - | chr3.1:88240190-88240209 | MS.gene014621:CDS | 50.0% | |
| TCGAATGGGAGTCTGTGGAA+GGG | - | chr3.1:88240245-88240264 | MS.gene014621:CDS | 50.0% | |
| TGAGGTAAGAGGAACCGAAG+AGG | - | chr3.1:88239901-88239920 | MS.gene014621:CDS | 50.0% | |
| AATGGGAGTCTGTGGAAGGG+TGG | - | chr3.1:88240248-88240267 | MS.gene014621:CDS | 55.0% | |
| GGAAGGTAAAGCCACCGTTG+AGG | - | chr3.1:88239883-88239902 | MS.gene014621:CDS | 55.0% | |
| GGTTCCTCTTACCTCAACGG+TGG | + | chr3.1:88239897-88239916 | None:intergenic | 55.0% | |
| TCACCACCGTCCATTTGCAG+TGG | + | chr3.1:88240199-88240218 | None:intergenic | 55.0% | |
| CTGCCACTGCAAATGGACGG+TGG | - | chr3.1:88240193-88240212 | MS.gene014621:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 88239854 | 88240360 | 88239854 | ID=MS.gene014621 |
| chr3.1 | mRNA | 88239854 | 88240360 | 88239854 | ID=MS.gene014621.t1;Parent=MS.gene014621 |
| chr3.1 | exon | 88239854 | 88240360 | 88239854 | ID=MS.gene014621.t1.exon1;Parent=MS.gene014621.t1 |
| chr3.1 | CDS | 88239854 | 88240360 | 88239854 | ID=cds.MS.gene014621.t1;Parent=MS.gene014621.t1 |
| Gene Sequence |
| Protein sequence |
