AlfalfaGEDB | MS.gene014694

M. sativa cultivar XinJiangDaYe / MS.gene014694

KEGG: K03439
GO: GO:0003674 GO:0003824 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008176 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360
PFAM: PF02390.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014694.t1	XP_003603585.1	99.2	255	2	0	1	255	1	255	7.60E-142	513.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014694.t1	Q8GXB7	79.8	243	46	2	8	249	8	248	2.5e-109	396.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014694.t1	G7J2W7	99.2	255	2	0	1	255	1	255	5.5e-142	513.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50496	MS.gene014694	PPI
MS.gene038492	MS.gene014694	PPI
MS.gene71227	MS.gene014694	PPI
MS.gene048631	MS.gene014694	PPI
MS.gene018116	MS.gene014694	PPI
MS.gene014694	MS.gene40390	PPI
MS.gene014694	MS.gene61042	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014694.t1	MTR_3g109360	99.216	255	2	0	1	255	1	255	0.0	528

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014694.t1	AT5G24840	79.835	243	46	2	8	249	8	248	1.12e-146	410

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTAAGTTTGCCGATAT+TGG	0.221557	3.1:+87165422	MS.gene014694:CDS
TCCGCACCACAGAAACATTT+TGG	0.250656	3.1:-87165586	None:intergenic
ATTCCGATCATCAATGTTTC+TGG	0.252188	3.1:-87165488	None:intergenic
GCCGATATTGGCTGTGGTTT+TGG	0.274610	3.1:+87165434	MS.gene014694:CDS
CCTCGTTTCTAGCAACCTTT+TGG	0.326676	3.1:-87165916	None:intergenic
TAAAATGAGGATCGGGAAAC+AGG	0.368009	3.1:-87165670	None:intergenic
TTCTCTTTAAAATGAGGATC+GGG	0.398927	3.1:-87165677	None:intergenic
GCATACTCATCTAGTAAGAA+TGG	0.400409	3.1:-87165725	None:intergenic
AAACGAGGGACAAACTTTCC+AGG	0.404412	3.1:+87165931	MS.gene014694:CDS
TTGTGCTTTCTCAAAGTGAT+TGG	0.404689	3.1:-87165630	None:intergenic
GGACTGGATGAAATCTTGTT+TGG	0.409104	3.1:+87165805	MS.gene014694:CDS
TCCAAAACCACAGCCAATAT+CGG	0.417152	3.1:-87165435	None:intergenic
TTAAGCTCTGCCACTGAAGA+AGG	0.428496	3.1:+87165893	MS.gene014694:CDS
TGTGCTTTCTCAAAGTGATT+GGG	0.444654	3.1:-87165629	None:intergenic
TCAACATTCAGTAAGTCAAC+AGG	0.452366	3.1:+87165248	MS.gene014694:CDS
GTGATTGGGAATGTACTTCA+TGG	0.454613	3.1:-87165615	None:intergenic
GTTGAATTTCCACCAGTCTC+AGG	0.455044	3.1:-87165230	None:intergenic
TGGTTCTTCTCTTTAAAATG+AGG	0.455252	3.1:-87165683	None:intergenic
CAGTCAAAGGTTCAAACAAC+GGG	0.464877	3.1:-87165835	None:intergenic
TAAAGTGTCCGAGTATGTTA+AGG	0.471708	3.1:+87165523	MS.gene014694:CDS
TCAAGTTCTTTCTCAGTCAA+AGG	0.473439	3.1:-87165848	None:intergenic
ATAACAGATGTGGAAGAACT+TGG	0.476880	3.1:+87165782	MS.gene014694:CDS
GCTCGATAGAAGCGCTTTCG+AGG	0.477207	3.1:-87165275	None:intergenic
TCTTTAAGGGTAGCAAATCC+AGG	0.486768	3.1:+87165557	MS.gene014694:CDS
GAAAGAACTTGAAGCAGATC+CGG	0.487319	3.1:+87165859	MS.gene014694:CDS
TTCCGATCATCAATGTTTCT+GGG	0.488618	3.1:-87165487	None:intergenic
AGAGAAGAACCATCGCCGTC+GGG	0.492041	3.1:+87165694	MS.gene014694:CDS
GAGGGAATAGTCAACATGAC+TGG	0.493842	3.1:-87165351	None:intergenic
CTTCTCTTTAAAATGAGGAT+CGG	0.494124	3.1:-87165678	None:intergenic
CATACTCATCTAGTAAGAAT+GGG	0.503167	3.1:-87165724	None:intergenic
TGCCACTGAAGAAGGCCAAA+AGG	0.507812	3.1:+87165901	MS.gene014694:CDS
TCAACATGACTGGGTGATAA+CGG	0.513146	3.1:-87165341	None:intergenic
AGGGAAAGAACTGAGGGTAA+TGG	0.514591	3.1:-87165373	None:intergenic
AAGTTTGCCGATATTGGCTG+TGG	0.514722	3.1:+87165428	MS.gene014694:CDS
GAAAGAACTGAGGGTAATGG+AGG	0.517145	3.1:-87165370	None:intergenic
TAACAGATGTGGAAGAACTT+GGG	0.526686	3.1:+87165783	MS.gene014694:CDS
AAGAGAAGAACCATCGCCGT+CGG	0.534336	3.1:+87165693	MS.gene014694:CDS
CTATCAGCTTTCTCAGACGA+GGG	0.534942	3.1:-87165392	None:intergenic
CAATGCGTCTGTATACAGCC+TGG	0.550730	3.1:-87165949	None:intergenic
GGCTGATAACCCGACGGCGA+TGG	0.555787	3.1:-87165703	None:intergenic
TAAACAGACATGCCTGAGAC+TGG	0.565306	3.1:+87165218	None:intergenic
ACCAAAATGTTTCTGTGGTG+CGG	0.567556	3.1:+87165585	MS.gene014694:CDS
AATTCGTTCCTTAACATACT+CGG	0.570956	3.1:-87165531	None:intergenic
TTCCCAGAAACATTGATGAT+CGG	0.572410	3.1:+87165485	MS.gene014694:CDS
GAGCTTAAAAGCTTAACAGC+CGG	0.573038	3.1:-87165878	None:intergenic
ATGTGGAAGAACTTGGGGAC+TGG	0.574117	3.1:+87165789	MS.gene014694:CDS
TGACTGGGTGATAACGGCAC+AGG	0.576851	3.1:-87165335	None:intergenic
AGAAACATTGATGATCGGAA+TGG	0.582107	3.1:+87165490	MS.gene014694:CDS
AAAGAACTGAGGGTAATGGA+GGG	0.599329	3.1:-87165369	None:intergenic
TATATACACAATAACAGATG+TGG	0.600775	3.1:+87165772	MS.gene014694:CDS
AACAGATGTGGAAGAACTTG+GGG	0.605004	3.1:+87165784	MS.gene014694:CDS
GGAAATGAGAGTCACTGAGC+GGG	0.605858	3.1:-87165313	None:intergenic
GACTGGGTGATAACGGCACA+GGG	0.611699	3.1:-87165334	None:intergenic
TCAGTCAAAGGTTCAAACAA+CGG	0.617042	3.1:-87165836	None:intergenic
TCAATACCAAAATGTTTCTG+TGG	0.618646	3.1:+87165580	MS.gene014694:CDS
CCAAAAGGTTGCTAGAAACG+AGG	0.621682	3.1:+87165916	MS.gene014694:CDS
AGGGAATAGTCAACATGACT+GGG	0.631622	3.1:-87165350	None:intergenic
ACAGACATGCCTGAGACTGG+TGG	0.638512	3.1:+87165221	None:intergenic
GGGAAATGAGAGTCACTGAG+CGG	0.640601	3.1:-87165314	None:intergenic
CAAAAGGTTGCTAGAAACGA+GGG	0.641444	3.1:+87165917	MS.gene014694:CDS
ACTATCAGCTTTCTCAGACG+AGG	0.648094	3.1:-87165393	None:intergenic
CAATGTTTCTGGGAACAGAG+TGG	0.655902	3.1:-87165477	None:intergenic
CAGACGAGGGAAAGAACTGA+GGG	0.659441	3.1:-87165379	None:intergenic
TCAGACGAGGGAAAGAACTG+AGG	0.695327	3.1:-87165380	None:intergenic
AGAATGGGCTGATAACCCGA+CGG	0.724299	3.1:-87165709	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATATACACAATAACAGATG+TGG	+	chr3.1:87165772-87165791	MS.gene014694:CDS	25.0%
!!	AGGAACGAATTTTATCTTTA+AGG	+	chr3.1:87165543-87165562	MS.gene014694:CDS	25.0%
!!	GGAACGAATTTTATCTTTAA+GGG	+	chr3.1:87165544-87165563	MS.gene014694:CDS	25.0%
	AAGATTAAGTTTGCCGATAT+TGG	+	chr3.1:87165422-87165441	MS.gene014694:CDS	30.0%
	AATTCGTTCCTTAACATACT+CGG	-	chr3.1:87165534-87165553	None:intergenic	30.0%
	CATACTCATCTAGTAAGAAT+GGG	-	chr3.1:87165727-87165746	None:intergenic	30.0%
!	TATGCTTTTGTTCTTGAGAT+TGG	+	chr3.1:87165743-87165762	MS.gene014694:CDS	30.0%
!	TCAATACCAAAATGTTTCTG+TGG	+	chr3.1:87165580-87165599	MS.gene014694:CDS	30.0%
!!	CTTCTCTTTAAAATGAGGAT+CGG	-	chr3.1:87165681-87165700	None:intergenic	30.0%
!!	TGGTTCTTCTCTTTAAAATG+AGG	-	chr3.1:87165686-87165705	None:intergenic	30.0%
!!	TTCTCTTTAAAATGAGGATC+GGG	-	chr3.1:87165680-87165699	None:intergenic	30.0%
	ATAACAGATGTGGAAGAACT+TGG	+	chr3.1:87165782-87165801	MS.gene014694:CDS	35.0%
	ATTCCGATCATCAATGTTTC+TGG	-	chr3.1:87165491-87165510	None:intergenic	35.0%
	GCATACTCATCTAGTAAGAA+TGG	-	chr3.1:87165728-87165747	None:intergenic	35.0%
	TAACAGATGTGGAAGAACTT+GGG	+	chr3.1:87165783-87165802	MS.gene014694:CDS	35.0%
	TCAACATTCAGTAAGTCAAC+AGG	+	chr3.1:87165248-87165267	MS.gene014694:CDS	35.0%
	TCAAGTTCTTTCTCAGTCAA+AGG	-	chr3.1:87165851-87165870	None:intergenic	35.0%
	TCAGTCAAAGGTTCAAACAA+CGG	-	chr3.1:87165839-87165858	None:intergenic	35.0%
	TGTGCTTTCTCAAAGTGATT+GGG	-	chr3.1:87165632-87165651	None:intergenic	35.0%
	TTCCGATCATCAATGTTTCT+GGG	-	chr3.1:87165490-87165509	None:intergenic	35.0%
	TTGTGCTTTCTCAAAGTGAT+TGG	-	chr3.1:87165633-87165652	None:intergenic	35.0%
!	AGAAACATTGATGATCGGAA+TGG	+	chr3.1:87165490-87165509	MS.gene014694:CDS	35.0%
!	TAAAGTGTCCGAGTATGTTA+AGG	+	chr3.1:87165523-87165542	MS.gene014694:CDS	35.0%
!	TTCCCAGAAACATTGATGAT+CGG	+	chr3.1:87165485-87165504	MS.gene014694:CDS	35.0%
!!!	GAAACATTTTGGTATTGACC+TGG	-	chr3.1:87165578-87165597	None:intergenic	35.0%
	AACAGATGTGGAAGAACTTG+GGG	+	chr3.1:87165784-87165803	MS.gene014694:CDS	40.0%
	AGGGAATAGTCAACATGACT+GGG	-	chr3.1:87165353-87165372	None:intergenic	40.0%
	CAAAAGGTTGCTAGAAACGA+GGG	+	chr3.1:87165917-87165936	MS.gene014694:CDS	40.0%
	CAGTCAAAGGTTCAAACAAC+GGG	-	chr3.1:87165838-87165857	None:intergenic	40.0%
	GAAAGAACTTGAAGCAGATC+CGG	+	chr3.1:87165859-87165878	MS.gene014694:CDS	40.0%
	GAGCTTAAAAGCTTAACAGC+CGG	-	chr3.1:87165881-87165900	None:intergenic	40.0%
	GGACTGGATGAAATCTTGTT+TGG	+	chr3.1:87165805-87165824	MS.gene014694:CDS	40.0%
	GTGATTGGGAATGTACTTCA+TGG	-	chr3.1:87165618-87165637	None:intergenic	40.0%
	TAAAATGAGGATCGGGAAAC+AGG	-	chr3.1:87165673-87165692	None:intergenic	40.0%
	TCAACATGACTGGGTGATAA+CGG	-	chr3.1:87165344-87165363	None:intergenic	40.0%
	TCCAAAACCACAGCCAATAT+CGG	-	chr3.1:87165438-87165457	None:intergenic	40.0%
!	ACCAAAATGTTTCTGTGGTG+CGG	+	chr3.1:87165585-87165604	MS.gene014694:CDS	40.0%
!	GCTTTTGTTCTTGAGATTGG+TGG	+	chr3.1:87165746-87165765	MS.gene014694:CDS	40.0%
!	TCTTTAAGGGTAGCAAATCC+AGG	+	chr3.1:87165557-87165576	MS.gene014694:CDS	40.0%
!!	AAAGAACTGAGGGTAATGGA+GGG	-	chr3.1:87165372-87165391	None:intergenic	40.0%
!!!	ATATTGGCTGTGGTTTTGGA+GGG	+	chr3.1:87165438-87165457	MS.gene014694:CDS	40.0%
	AAACGAGGGACAAACTTTCC+AGG	+	chr3.1:87165931-87165950	MS.gene014694:CDS	45.0%
	ACTATCAGCTTTCTCAGACG+AGG	-	chr3.1:87165396-87165415	None:intergenic	45.0%
	AGGGAAAGAACTGAGGGTAA+TGG	-	chr3.1:87165376-87165395	None:intergenic	45.0%
	CCAAAAGGTTGCTAGAAACG+AGG	+	chr3.1:87165916-87165935	MS.gene014694:CDS	45.0%
	CTATCAGCTTTCTCAGACGA+GGG	-	chr3.1:87165395-87165414	None:intergenic	45.0%
	GAGGGAATAGTCAACATGAC+TGG	-	chr3.1:87165354-87165373	None:intergenic	45.0%
	GTTGAATTTCCACCAGTCTC+AGG	-	chr3.1:87165233-87165252	None:intergenic	45.0%
	TTAAGCTCTGCCACTGAAGA+AGG	+	chr3.1:87165893-87165912	MS.gene014694:CDS	45.0%
!	AAGTTTGCCGATATTGGCTG+TGG	+	chr3.1:87165428-87165447	MS.gene014694:CDS	45.0%
!	GAAAGAACTGAGGGTAATGG+AGG	-	chr3.1:87165373-87165392	None:intergenic	45.0%
!	TCCGCACCACAGAAACATTT+TGG	-	chr3.1:87165589-87165608	None:intergenic	45.0%
!!	AACCTTTTGGCCTTCTTCAG+TGG	-	chr3.1:87165906-87165925	None:intergenic	45.0%
!!	CAATGTTTCTGGGAACAGAG+TGG	-	chr3.1:87165480-87165499	None:intergenic	45.0%
!!!	CCTCGTTTCTAGCAACCTTT+TGG	-	chr3.1:87165919-87165938	None:intergenic	45.0%
!!!	GATATTGGCTGTGGTTTTGG+AGG	+	chr3.1:87165437-87165456	MS.gene014694:CDS	45.0%
	AAGAGAAGAACCATCGCCGT+CGG	+	chr3.1:87165693-87165712	MS.gene014694:CDS	50.0%
	ATGTGGAAGAACTTGGGGAC+TGG	+	chr3.1:87165789-87165808	MS.gene014694:CDS	50.0%
	CAATGCGTCTGTATACAGCC+TGG	-	chr3.1:87165952-87165971	None:intergenic	50.0%
	CAGACGAGGGAAAGAACTGA+GGG	-	chr3.1:87165382-87165401	None:intergenic	50.0%
	GGAAATGAGAGTCACTGAGC+GGG	-	chr3.1:87165316-87165335	None:intergenic	50.0%
	GGGAAATGAGAGTCACTGAG+CGG	-	chr3.1:87165317-87165336	None:intergenic	50.0%
	TCAGACGAGGGAAAGAACTG+AGG	-	chr3.1:87165383-87165402	None:intergenic	50.0%
	TGCCACTGAAGAAGGCCAAA+AGG	+	chr3.1:87165901-87165920	MS.gene014694:CDS	50.0%
!!	AGAATGGGCTGATAACCCGA+CGG	-	chr3.1:87165712-87165731	None:intergenic	50.0%
!!	GCCGATATTGGCTGTGGTTT+TGG	+	chr3.1:87165434-87165453	MS.gene014694:CDS	50.0%
	AGAGAAGAACCATCGCCGTC+GGG	+	chr3.1:87165694-87165713	MS.gene014694:CDS	55.0%
!!	GACTGGGTGATAACGGCACA+GGG	-	chr3.1:87165337-87165356	None:intergenic	55.0%
!!	GCTCGATAGAAGCGCTTTCG+AGG	-	chr3.1:87165278-87165297	None:intergenic	55.0%
!!	TGACTGGGTGATAACGGCAC+AGG	-	chr3.1:87165338-87165357	None:intergenic	55.0%
	GGCTGATAACCCGACGGCGA+TGG	-	chr3.1:87165706-87165725	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	87165227	87165994	87165227	ID=MS.gene014694
chr3.1	mRNA	87165227	87165994	87165227	ID=MS.gene014694.t1;Parent=MS.gene014694
chr3.1	exon	87165227	87165994	87165227	ID=MS.gene014694.t1.exon1;Parent=MS.gene014694.t1
chr3.1	CDS	87165227	87165994	87165227	ID=cds.MS.gene014694.t1;Parent=MS.gene014694.t1

Gene Sequence

>MS.gene014694

ATGCCTGAGACTGGTGGAAATTCAACATTCAGTAAGTCAACAGGATTGCCTCGAAAGCGCTTCTATCGAGCACGAGCACACAGCAACCCGCTCAGTGACTCTCATTTCCCTGTGCCGTTATCACCCAGTCATGTTGACTATTCCCTCCATTACCCTCAGTTCTTTCCCTCGTCTGAGAAAGCTGATAGTTGCAAAAAGATTAAGTTTGCCGATATTGGCTGTGGTTTTGGAGGGTTGCTCATCAGTCTTTCCACTCTGTTCCCAGAAACATTGATGATCGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTTAAGGAACGAATTTTATCTTTAAGGGTAGCAAATCCAGGTCAATACCAAAATGTTTCTGTGGTGCGGACTAACTCCATGAAGTACATTCCCAATCACTTTGAGAAAGCACAACTCTCAAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGCCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTTTGTTCTTGAGATTGGTGGAATTATATACACAATAACAGATGTGGAAGAACTTGGGGACTGGATGAAATCTTGTTTGGAGAATCACCCGTTGTTTGAACCTTTGACTGAGAAAGAACTTGAAGCAGATCCGGCTGTTAAGCTTTTAAGCTCTGCCACTGAAGAAGGCCAAAAGGTTGCTAGAAACGAGGGACAAACTTTCCAGGCTGTATACAGACGCATTGCAGCATCTGATCAAATATCCTAG

Protein sequence

>MS.gene014694.t1

MPETGGNSTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPLSPSHVDYSLHYPQFFPSSEKADSCKKIKFADIGCGFGGLLISLSTLFPETLMIGMELRDKVSEYVKERILSLRVANPGQYQNVSVVRTNSMKYIPNHFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAFVLEIGGIIYTITDVEELGDWMKSCLENHPLFEPLTEKELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIAASDQIS